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Presented By:-
1. DEFINITION
2. METHODS
CHEMICAL METHODS
INHALANT
NON-INHALANT
PHYSICAL METHODS
Definition:-
1. CARBON DIOXIDE:-
● An effective and widely used agent to euthanize rodents.
● It is dangerous to use, so precaution should be taken while
using it.
2. Nitrous oxide (N2O):-
● Not preferred due to lack in fast onset.
● May produce hypoxemia and cardiac or respiratory arrest.
3. Ether:-
● It was formerly used extensively, but is now only acceptable
conditionally.
● Reason is being irritant to mucous membranes and risk of fire and
explosion.
NON-INHALATIONAL
1. Barbiturates:-
● Sodium pentobarbital is the most rapid and reliable method of
euthanasia for most experimental animals
● In non-rodent species, given intravenously to be most effective.
Intraperitoneal injection is acceptable for euthanasia in small
mammals.
2. Decapitation:-
● Used to euthanize rodents and small rabbits.
● Guillotine is generally used.
Guillotine
3. Microwave irradiation:-
● Used when an experiment requires fixation of mouse or rat brain
metabolites in vivo without losing anatomic integrity of the brain.