ISO 21867:2004(E) Annex C (normative) Description of Shigella colony morphology and colour on selective agars, for both identification and quality control purposes All Enterobacteriaceae on all these media have round colonies with a smooth surface and entire edge. The size of well-isolated colonies is generally > 2 mm. See Table C.1 for details, Table C.1 — Description of Shigella colony morphology and colour on selective agars Bacterial species MacConkey agar ‘Selective agars XLD agar Hektoen agar Shigetta sonnei ® [Colourtess to pate pink translucent, lactose negative Translucent with redicerse| centre, same colour as the| agar [Green and moist raisea| colonies. Shigeta, other species (Colouness,transtucent, lactose negative, Translucent with redleerse| centre, same colour as the| agar. [Green and moist Escherichia col Red with turbid precpitate in ager. Yellow, opaque, surrounded by yellow precipitate in agar. Redlsaimon with zone off precipitate in ager. agar yellow around colony. Enterobacter Red with turbid precipitate in| Yellow, opaque, surrounded [Redisalmon with zone of cloacae ager. by yellow precipitate in agar |preciptation agar. Kiebsiela Red with turbid precipitate in| Yellow, opaque and mucoid, [Redisalmon with zone of pneumoniae ager. surrounded by yellow Precipitation agar precipitate, Salmonella [Colourtess and translucent [Red with Black centre, Blue green, with or without black cent. Proteus mirabilis (Colourless and translucent ager yellow around colony, Yellow, black centre, yelow agar wth precptate, Bluelgreen, with or without black cent Enterococcus faccalis Red, small round colonies. ‘None or poor grow, colonies yellowish None or poor growth, colonies yellowish. ‘Shige sana na foment ose ater > 49 ncn, resuing in a weak reaction sir to Each ray aueeglnate upon suspen marie wit) ass of @ 120 moadaton pasa and aloe a ater tsi, Sti 26 18180 2004 ~ Alright reserved