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oeTHE EFFECTIVE USE
AND PROPER CARE OF
THE MICROTOME
by
Oscar W. Richards, Pd. D.
Chief Biologist
American Optical Company
SPENCER
COPYRIGHT 1942
COPYRIGHT 1949
COPYRIGHT 1959
American @ Optical
Instrument Division
Buffalo 15, New York
One DollarTable of Contents
I, Introduction . . . Gow a ye ws 8 ew
Il. Correcting Difficulties Hacounteredt in Sectioning
A. Difficulties Common to All Methods. . . 1. «
B. Difficulties of Special Methods. . . . 2...
Paraffin Embedded Material. . . . . .
Frozen Section Technic. . . . . 2 1 se
Celloidin Embedded Material . . . . .
III. Directions for the Use of the AO Spencer No. 820 Rotary
Mawes 6 5 0 oo eo 6 8 le as
A. Use. B. The Knife Holder.
C. Thin Section Adapter. D. Maintenance.
IV. Directions for the Use of the AO Boenert No. 815 Holey,
Microtome ....
V. Directions for the Use of the AO Spencer No. 360 Siding
Microtome :
VI. The Aieovome Knife
Dlistarigal ees 1 ae ee ae
2 Experimental. 2 2 2 0 ee
8 HandSharpening . .. . . 1... s
VII. Methods
1. Theory of Cutting . . . ae
Positioning the Knife in the Microtome :
Table Microtome ,
Freezing Technic .