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ae | U R P ll t=] ed on ieee — _— J el — oe THE EFFECTIVE USE AND PROPER CARE OF THE MICROTOME by Oscar W. Richards, Pd. D. Chief Biologist American Optical Company SPENCER COPYRIGHT 1942 COPYRIGHT 1949 COPYRIGHT 1959 American @ Optical Instrument Division Buffalo 15, New York One Dollar Table of Contents I, Introduction . . . Gow a ye ws 8 ew Il. Correcting Difficulties Hacounteredt in Sectioning A. Difficulties Common to All Methods. . . 1. « B. Difficulties of Special Methods. . . . 2... Paraffin Embedded Material. . . . . . Frozen Section Technic. . . . . 2 1 se Celloidin Embedded Material . . . . . III. Directions for the Use of the AO Spencer No. 820 Rotary Mawes 6 5 0 oo eo 6 8 le as A. Use. B. The Knife Holder. C. Thin Section Adapter. D. Maintenance. IV. Directions for the Use of the AO Boenert No. 815 Holey, Microtome .... V. Directions for the Use of the AO Spencer No. 360 Siding Microtome : VI. The Aieovome Knife Dlistarigal ees 1 ae ee ae 2 Experimental. 2 2 2 0 ee 8 HandSharpening . .. . . 1... s VII. Methods 1. Theory of Cutting . . . ae Positioning the Knife in the Microtome : Table Microtome , Freezing Technic .

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