RS-232 SPECIFICATION for Congulation System ‘el 300/ 3000 Family Instrumentation Laboratory Software Department Document: RS232_5DOC ‘Author Date Revision Comments L. Peckelsen 10-44 Original document and updates S. Vaghi-E. Lattuada 20 Dec 94 5.0 Update for software revision 09 11 Jan 95 sa ‘Modifications on tests code 3 Jan 96 52ACL, Step III, Phase B: RS-232 Specification Table of contents IMUEOGUCELONs sees ees eese eee eeeeeeeeeeu ete ese een etenseneeea eee eed ‘Transmission types. a4 2.1 Interface configurations settee eet e eee e eee PROG DATA TRANSMISSION... ssssesseesseveeseseuesee eee eeees® 2.3. PROG INTERFACE STATUS. 8 :4 Instrument configurations. . 26 Data transmission procedures. ...scseeeeeeeeeeeeeeeeeceneee eee eeT 3.1 Communication procedure for the IL protocol. 212 3.2 Communication sequence for the IL protocol. 214 3.3. Interconnect cablesssssesssesseseeeaesesceseeeesen esse ese 016 Formal description. 17 4.1 ‘Transmission types r19 4.2 Reception typessesseseesecseceeeseeeeeeeteeeeeeeneen eer 4.3 protocol description. 0 4.4 Message description. tieteteeeceeseessees 5.1 description....sscssicssssssseesereeeee2@ 5.2 <¥,x> description. 231 5.3 description. 232 5.4 description. . 233 5.5 description. ......seccecceeeeeccescu eee ecen scenes edd 5.6 descriptions. s.ssveeeeseeeeeeeeeceneteeeeeeee teense e135 5.7 description. 237 Instrumentation Laboratory, October 1999ack, Step III, Phase 2: AS~: specification Revisions Phase A 19 Mar. 91 SW Release rev. 00 - 15 Lug. 91 ~ Modificato sostituendo i parametri relativi all‘elaborazione dati con spare. = sostituita 1'indicazione 'no def. plasma’ con 'no reagent 18". 26 Ago. 91 - Uniformato il formato per i risultati nei messaggi che ora non difterenzia piu’ tra valori integer e real. 6 sett. 91 - Bliminata 1'informazione FIB ON/OFF (equivalente della selezione nel PROG PRINTOUT FORMAT) nel campo che non viene gestito dal swe che non veniva mai transmesso. SW Release rev. 01 ~ Phase 8 27 February, 1992 - aggiunti i parametri per i cicli FIBRINOGEN-c, PROCHROM, PROTEIN-S nei seguenti capitolo: capitolo 4.4: nota a, nota a capitolo struttura della trasmissione capitolo struttura risultatl <¥,x> capitolo struttuta capitol struttura capitolo struttuta capitolo struttura ~ Sastituzione del nome Procomplex con Pro-IL-Complex. 6 gune 94 - Tutti i documenti di specifica sono "congelati" con 1a label revisione 4.4, associata al rilascio della Rev.sW 08 20 December 94 - New transmission codes and data transmission format introduced for PX, PCx and ATIII. - Transmission codes for P-C and P-S, the new cycles of rev. 09, were already available. 12 January 95 - Transmission code for ATITI, HPX and PCX modified 3 ganuary 9 - Message contents: messages sequence modified for AT-III, PRO-IL~ COMPLEX and HEPATOCOMPLEX Analysis - message description: code 5p and 60 removed because not meaningful Instrumentation Laboratory, October 2: 199s 3ACL, Step III, Phase B: RS-232 Specification RS-232 Specification 4. Introduction This document describes the serial interface characteristics of the ACL coagulation system. The purpose of this document is to supply, in sufficient detail, the information required to facilitate the development of computer programs that will receive data from an ACL, Furthermore, the transmission procedures and the formats are explained to interface an external computer witn the ACL. Chapter 2 gives an overview of the different kinds of data transmissions and their availability on the various models of the ACL line. chapter 3 lays out how data are formatted, transferred and re-transmitted if errors occur. 2. Transmission types A transmission is a communicating entity that includes all the acquired raw data ox the analytical results involved in a cycle. The ACL-line has two types of data transmission: 1.) transmission of analytical results, 2.) transmission of raw data. Transmission of analytical results in unformatted form is available on all models and provides the instruments with the possibility of transmitting tne analytical results (e. g. samples, pools and 1Ds) to an external computer for further processing, like Quality Control or Data Management systems. wot! rhe transmission of analytical results is a superset of the first ACL generation one. This means, all software written for the ald ACL will also run with the new line with the exception of newly introduced features. only the ACL-300/R and ACL-3000+ are equipped with a software package that allows raw data transmission for all analytical cycles. The acquired non- elaborated data can be sent to a PC compatible where they are memorised and processed with a dedicated software package, WINDOWS-RESEARCH. The transmission is not intended for general public use and the transmission format is not published. WINDOWS-RESZARCH software is exclusively offered by Instrumentation Laboratory. 4 Instrumentation Laboratory, October 1999ACL, Step III, Phase RS-232 Specification 2.1 Interface configurations ‘The models of the ACL line are equipped with woth interfaces can be independently enabled various requirements. Interface 1/2 corresponds to the lower/upper panel. 2.2 PROG DATA TRANSMISSION in the on the ‘The transmission of analytical results activated in different ways, dependent “DATA TRANSMISSION": oFF ON WITH AUTOMATIC TRANSMISSION ON WITHOUT AUTOMATIC TRANSMISSION 2.3 PROG INTERFACE STATUS The ACL uses asynchronous one-direction tran; characteristics are configurable in the PROG PROTOCOL BAUD RATE CHARACTER LENGTH PARITY STOP BITS Interface 2 is dedicated to special applicat: Instrumentation Laboratory, Octobe: 1 or 2 serial RS-232¢ interraces. and configured to satisfy the connector located on the ACL rear @ RESULT phase of a cycle can be @ operator selection in the PROG no transmission at all automatic transmission as soon as all results are available transmission on demand pressing the "S"-key in the RESULT phase mission. The following "INTERFACE STATU: Tbe 110, 1200, 7 Bits, NONE, ODD, a, 1.8, 2 IBM, XON/XOF®, DIR 150, 300, 600, 2400, 4800, 9600 = BITS EVEN dons like RESEARCH. 22, 1999ACL, Step III, Phase B: RS-232 Specification 2.4 Instrument configurations ACL-300/R, 3000+ The top model has two interfaces of type DTZ, standard 25 pin female connectors. the analytical results can be transmitted via one or both interfaces. Both interfaces can independently be enabled and configured hy the operator. The raw data in a RESEARCH cycle are always transmitted on interface 2 to an external PC. In this case, the interface set-up is predefined. the selections in the PROG INTERFACE STATUS 2" should bet IL - 9600 - 7 BITS - ODD - 1 BIT. Ach. the base model has two interfaces of type DIE, standard 25 pin female connectors. the analytical results can be transmitted via one or both interfaces. Both interfaces can independently be enabled and configured by the operator. ACL=200, 100, 2000, 1000 hese models have one interface of type DTZ, standard 25 pin female connector. the analytical results are transmitted via interface 1. The interface can be enabled and configured by the operator. provision has been made for an optional cycle selectable curve that transmits aw data to a PC, equipped with a special software package. The raw data are transmitted on interface 2 for the ACL-300 and 3000, All other models use interface 1. For additional information refer to the operator's manual section Interface Status, Data Transmission. 6 Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ specification 3. Data transmission procedures ‘The following chapters mainly explain the transmission of analytical results, that requires detalled knowledge for Interfacing the ACL with an external computer. Application, Presentation Layer The analytical results of 4 run with up to 19 samples plus POOL are transmitted in messages called message Header and Message Contents. Each transmission starts with a Message Header followed by a sequence of messages called Message Contents (see chapter 4.1). ‘The Message Header contains information of a general nature valid for the whole transmission (test identifier, date, time, warnings, batch no.) and two important data: 1.) number of messages of the type Message Contents to follow, 2.) parameter to distinguish analytical result transmission from other transmission types. ‘The Message Header has a fixed length of 52 characters. Its format is explained in chapter 4.4. ‘The Message Contents contains the data that can be results, identifiers or calibration data, The last two characters in the Message contents are used to identify the data type. The data in a message are sent in a compressed form without data separators and without units. If no numerical result is available, the Ist position contains an error code. ‘The Message Contents has a fixed length of 29 characters. Ite format for the different analytical cycles is explained in chapter 5. ‘The transmission of the messages is sequential and can only be interrupted by operator intervention, like STOP-key. In this case, an abort code (ASCII control char. Eor= 04) is sent over the serial interface (see chapter 4.1) and a pata Transmission Error appears (see PROG "WARNING"). This warning is also displayed, if the software detects an error during transmission. In the last case, the transmission can be repeated by pressing the "9"-key during the RESULT phase. A successful transmission cancels the warning and the possibility of ve- transmitting the results. one special transmission is performed in the PROG "DELETE SAMPLE ID/BATCH NO.", where one message called delete SAMPLE ID or delete BATCH NO. is sent to the external computer. The length of this message is 16 characters (see chapters 4.1 and 4.4). All data in the messages are printable ASCIT characters (32..127). Instrumentation Laboratory, October 22, 1999 1ACL, Step III, Phase B: RS-232 Specification session Layer The ACL systems are provided with a suite of protocols to synchronize and organize the dialogue and to manage the data exchange: IL handshake protocol, TBM handshake protocol, X-off/X-on flow control protocol, DIR flow control protocol The messages are surrounded by package identification and error checking information, called protocol header and protocol tail (see chapters 4.2 and 4.3). Protocol header, message and protocol tail form an entire data package. The IL protocol is the standard protocol for interconnecting an ACL with an external computer (e. g. PC). In this protocol the ACL sends additional information to the recipient in order to identify the instrument originating the tvansmission (instrument ID), to keep track on the data packages sent/received (sequence no.) and to ensure data integrity (checksum). The protocol uses asynchronous communication: waiting for a response (acknowledge from the external computer) before sending the next data package. During a transmission, each data package must be validated by the external computer. The ACL waits for an acknowledge (ACK) or negative acknowledge (NACK) before sending the next data package. If an ACK is received by the ACL, the ACL continues transmission with a sequence no. incremented by one. If the ACL receives a NACK or no reply within 30 sec, it attempts to re-transmit the data package with the same sequence no, If the second attempt fails, the transmission is terminated and the sequence number is incremented. A Data Transmission Error is displayed in the PROG "WARNINGS" An external computer can break the transmission by sending two NACK messages to the ACL. A Data Transmission Error is displayed. In case of a Data Transmission Error, the transmission can be repeated manually, ©. g. by pressing the "9"-key during RESULT phase. A successful transmission cancels the warning. 8 Instrumentation Laboratory, October 1999ACL, Step III, Phase B: RS~ specification ‘The IgM protocol is implemented for compatibility with old instruments. It is not recommended for future developments. ‘The protocol uses asynchronous communication: waiting for a start sequence from the external computer before sending a data package. During a transmission, each data package must be requested from the external computer through the start sequence . After having received the start sequence, the ACL transmits one data package composed of is composed of the following fields: estart> + + + + + (see chapter 4.3). - A can be of different types: | | | | | (see chapters 4.4 and 5). = The contains the (see chapter 4.3). Bach data package starts with the start-char. (ASCTI :) of the fixed length protocol header (17 chars, start included). The protocol header contains a field specifying the actual length of the succeeding messages . The data package is terminated by a fixed length protocol tail (2 char-s). the also identifies the type of the message: length 52: , length 28: , length 16: or message. The is set to zero at power-on and wraps around to zero when it exceeds its max. value 65536. The checksum is the two's complement of the sum of all char.s in the data package excluding the start char. and the checksum itself. The checksum is calculated modulo 256 and presented as 2 HEX-ASCII char.s, that means the decimal range 0 to 255 is transformed into the hexadecimal range 00 to FF. Note: Sequence #, message length and checksum are presented as HEX-ASCIT characters, ©. g., a decimal number of 9326 is converted to hexadecimal number of 14cz, that is transmitted as an ASCII string of 4 char.s "14ce". 10 Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ specification physical Layer 1.) Interface type: DTZ, 25 pin female connector pin description BIA _ccrrt z Gd Protective Gad ~ AB LOL 2 TxD Transmitted Data output BA 103 3 RxD Received Data input BB 104 4 RIS Request to Send output ca 105 5 crs clear to send input cB 106 é DSR «Data Set Ready input co 107 7 cnd signal Gnd - AB 102 20 DIR Data Terminal Ready output ep 108.2 ) Physical signal characteristics Protective Ground Tied to ACL chassis ground. Transmitted Data output; data from acL to external device. Received pata input; data from external device to ACL. Request to Send outputs set true by ACL software when ready to receive data (e. g. ACK). clear to send input; True indicates to act that a char. may be sent to the external device. False indicates to ACL that no new transmission may begin. acu will complete transmission of the current character and, if present, any char. in the USART's (8251) char. buffer. That means that a maximum of two char.s may be sent from the ACL to the external device after Clear to Send goes false. Line is pulled-up with a resistor of 10 Kohm. Data set Ready Input; not used by ACL. signal Ground The signal reference level. Data Terminal Ready Outputs True indicates to the external device that the acL is ready to receive a character. The ACL holds this pin true permanently from the beginning of the first transmission. ‘True/False corresponds to a range from -5V to -15V/+5V to +15V. Instrumentation Laboratory, October 22, 1999 anACL, Step III, Phase B: RS-232 Specification 3.1 Communication procedure for the IL protocol The ACL starts transmission whenever the CTS line is round true (nigh level). 1, step The external computer (receiving unit) should synchronize itself to the character. Once recognized, the receiving unit should read the whole protocol header (17 char.s) and then fetch the from it. This parameter plus the fixed protocol tail length (2 char.s) establish the total number of char.s to be received for this data package. The ACL stops sending char.s and expects a response (ACK, NACK) within a fixed timeout pericd of 30 sec. (see chapter 4.3). 2. step First, the receiving unit has to verify the correctness of the data inside the data package. This is performed by calculating the checksum and comparing it with the transmitted one. If the values are not equal, the data package is rejected as erroneous and a NACK is returned to the ACL. If the two values correspond, the received data are correct. Second, the should be verified to determine the type of the message. Values other than 52 (34H), 28 (ICH) or 16 (10H) are invalid ana cause the transmission of a NACK to the ACL. The receiver returns to step 1, waiting for the next data package. If the received message is a delete SAMPLE ID or delete BATCH NO. (len.- 16), the receiving unit returns an ACK. The transmission from the ACL is terminated. If the received message is a Message Header (len.- 52), the receiving unit should memorize the determines the number of messages that follow to complete the transmission. The receiving unit returns an ACK to the ACL and waite for the next data package (see chapter 4.3). Tf the received message is a Message Contents (len.= 28), the receiving unit should control the sequence # to synchronize itself to the transmission. In normal continuous transmission, the sequence f will increment by one. An increment by zero means re-transmission of a data package (erroneous or redundant package). Both cases generate an ACK message. If the increment is greater than 1, then data have been lost and a NACK is sent to the ACL. Transmission is completed, if the actual sequence # has reached its rinal calculated value (initial sequence # plus f messages). wot! with 9600 baud the ACK/NACK sequence should be sent with a minimum delay of 10 ms between each char.! ACL can buffer up to 16 bytes before running in overflow. Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ specification 3. step After having received the ACK/NACK sequence, the ACL resumes operation after 100 millisec. In case of NACK, the data package is re-transmitted with the same sequence number. otherwise, the sequence numper is incremented. ‘The previous steps are repeated until all data packages are transmitted correctly over. Instrumentation Laboratory, October 22, 1999 13ACL, Step III, Phase B: RS-232 Specification 3.2 Communication sequence for the IL protocol ACL-SIDE EXT, COMPUTER | Protocol Header | Message Header | Protocol Tail | --> | len.= 17 I len.= 52 | len.= 2 bs 0017830000034, 1p048 6. MAY. 86 14: 01 0 1 | ACK within 305 | | len.- 5 1 10017 | Protocol Header | | Protocol Tail | I len.= 17 I len.= 28 | len.= 2 1 100188300000016%),, 13.7 200 *24.3 23 ~ | ACK within 30s | ro018 | Protocol Header | | protocol Tail | 00198300000016%)%, 123456 50,0, e.g. data lost 29 ~ | NACK within 305 | | protocol Header | | Protocol Tail | > (ze-transmission) 00198300000016,%, > 09, 123456 502p%, 23 10019 | protocol Header | | Protocol Tail | --> :00Las 30000001692, 14.2 89 1.04 199 019%, 713 | ACK within 30s | toola 1a Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ Specification | Protocol Header | | Protocol Tail | --> 1001883000000100)% 24.6 0199, 55 <-- | ACK within 30s | 1001p Communication sequence, interpretation pata are received from an ACL. instrument ID>= 63 Transmission starts with sequence # 0017. sequence #>= 0017 Transmission terminates after sequence # 001R. initial seq.# + <# messages> Results are from the mixed cycle PT-FIB/APTT, performed with standard acq. time = 1B, = 4 units of FIB is mg/dl, ratio is expressed as INR with an Ist of 1.012 = &, = 1.012 cycle was performed on May 8, 1996 at 14:01 , No warnings are indicated during the cycle. swarnings>= 00, = 0 All results are members of the batch no. 12345678901 ‘The NP yields a PP of 13.7, a FIR of 200mg/dl with message id= €0 and an APTT of 24.35 that is out of range. ‘The NP identifier is 123456. with message id= 50 ‘The first sample has the following results: <> with message id= 01 Pr 14.28, 89%, 1.047NR FIB199mg/d1. APTT 24.65, ratio not available Note: The presented checksums are not real ones and only serve for demonstration purposes. Instrumentation Laboratory, October 22, 1999 asACL, Step III, Phase B: RS-232 Specification 3.3 Interconnect cables 1. ACL to nost interconnection: AcL-side Host-side Interface type: DTE Interface type: DIE 25 pin connector 25 pin connector pin__description cable pin description 1 protective Gad — 2 Protective Gnd (optional) 2 TaD 3 RxD 3 RxD 2 TxD 4 RTS 5 crs 5 crs 20 DIR 6 DSR i, signal cnd 1 signal cnd 20 DIR 6 DSR The cable in the configuration male - male is offered by Instrumentation Laboratory. ACL to PC-AT interconnection: AcL-side pc-side Interface type: DIE Interface type: DIE 25 pin connector 9 pin connector pin__description cable pin description 1 protective Gad — 2 Protective Gnd (optional) 2 TxD 2 RxD 3 RxD 3 TxD 4 RTS 8 crs 5 crs 4 DIR 6 DSR i, signal cnd 5 signal cnd 20 DIR é DSR The 9 pin connector (male) is used in pc-aT models. he physical signals are explained in chapter 3, part physical Layer. ie Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ specification 4. Formal description ‘The ACL communicates with an external computer in an artificial language. For describing the syntax of tnis artificial language, the Backus-Naur Form (BNF) is a useful means. This document applies an BNF-like specification to give a complete and succinct description. legend: rr= may be composed of <> syntactic category (composed) text syntactic element (not composed) + and 1 or (xp... the expression (x) may be repeated n(x} the expression (x) may be repeated a max. number of times (a= 0 to max.) (+12) optional data Hexadecimal numbers are used in this document with the suffix #, e.g. 12H. For ASCIT, the characters are enclosed in apostrophes ("). HEX-ASCIZ char.s are the ASCII representation of hexadecimal numbers (0..9, A.+F) HEX-ASCII char. 1 1 30H ven 3en 31a vor= 30H 328 A= 41H 338 van 42H 24H wens 43H 358 D"= 44H ne" 36H En 45H "ym 378 "Ete 46H HEX-ASCIZ string is composed of a number of HEX-ASCII char.s, e.g. "19AS" means 31H, 39H, 41H, 46H. The string is transmitted from left to right, e. g. "19": first 31H then 39H. Instrumentation Laboratory, October 22, 1999 vvACL, Step III, Phase B: RS-232 Specification 4.1 Transmission types | «trans. delete SAMPLE ID> | | | | sabort> = + + + 38{ + + } protocol header> + + «protocol tail> protocol header> + + + + protocol tail> + + + + 20( + + } «trans. delete BATCH No.> not for public use not for public use + + . ACL RS232 CHECK" + TRANSMISSION COMPLETED CORRECTLY" + + message service> 2 ASCII char.s: ODH, OAH 1 ASCII char.: 04H 1s. Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ 4.2 Reception types <1BG-cR> ‘ Note: The OR char. (ODA) Instrumentation Laboratory, specification | | | | | | fe. get “IASE*= 1 5 HEX-ASCII char <1BG-CR> 2 ASCII char. 2 ASCII char. 2 ASCII char.s: 2 ASCII char. 1 ASCIT char: 1 L ASCII char. 1 empty in the IBM protocol ij october 22, 5 HEX-ASCII char. 4 HEX-ASCII char. 1999 8: "1" + AQPH= 6815 sen "12772" ica, opH 134, 0DE 11a, opH 17a, oDH 38 an 5 optional. ag.ACL, Step III, Phase B: RS-232 Specification 4.3 Protocol description «protocol header> | | protocol header XON/XOFE> | + + + + + length: 17 bytes 1 ASCIT char.: ":" (3AH) 2 ASCII char.s: "93" (for AcL-1000 and AcL-100 family) «filler p> fie 4 RSCIT char.s: "0000" (reserved) 4 HEX-ASCII char.s e.g. "0034", 52 message header 28 message contents 1€ delete BATCH NO. 16 delete SAMPLE ID 2 ASCII char.s: ODH, OAH ‘<18G-CR> 2 ASCIT char.s: 1cH, ODH empty «protocol header prR> empty protocol tail> protocol tail 1b | protocol tail IBM> | | «protocol tail IL> length: 2 bytes 2 HEX-ASCIT char.s (1 byte binary value) see below eprotocol tail IEM> <2IN-cR> 2 ASCII char.s: 17H, ODH 2 ASCIT char.s: ODH, OAH = 2 ASCII char.s: ODH, OAH Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ specification The checksum is two ASCII char.s representing a hex number calculated as follows: sum of all characters in the and the excluding the char., sum modulo 256 plus carry, take the two's complement of the result. Checksum calculation example cheeksum carry = 0) poi=170m Joop on all char.s/ checksum = checksum + chararray(i) + carry; /char-s sum/ carry = 07 IF (checksum > 255) THEN DO; carry = 17 checksum = checksum MopuLE 256; END: END? checksum = ((NOP checksum) + 1) MODULE 256; jtwo's complement/ oF Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS-232 Specification 4.4 Message description stest ID> Note: a) by ° «message header> | «message contents> | | | | + <# messages> + + + + + + + + + + length: 52 bytes 2 HEX-ASCII char.s: test identifier "90" see below "01" undefined test (for internal use) "02" see below 03" pr-FIB "oe" ApTT vos" Tr "OF" EXTRINSIC PATHWAY "15" INTRINSIC PATHWAY "1p" PY-PIB/APTT TT/apTT "21" SINGLE FACTOR - EXTRINSIC, see note a) "24" SINGLE FACTOR - INTRINSIC, see note a) "27" DOUBLE PT-FIB "2a" DOUBLE APTT "2D" DOUBLE TT 26" ARS.: ANTITAROMBIN IIT-ANALYSIS, see note d) "30" ABS.: ANTITAROMBIN III (in-run calibration; used up to rev. 08), see note d) "a1" ARS.t FIBRINOGEN-C "32" ABS.: PROCHROM. "33" ABS.! HEPARIN "aa" aRS.: for future use (3) "35" ABS.: for future use (4) "36" ABS.: ANTIPLASMIN "39" ARS.: PLASMINOGEN "43" SPECIAL: PRO-IL-COMPLEX-ANALYSIS, see note e) "4c" SPECIAL! HEPATOCOMPLEX-ANALYSIS, see note f) "51" SPECIAL: PROCLOT "@3" DOUBLE PY-FIB/APTT "6c" SPECIAL! PROTEIN-S "ep" SPECIAL: for future use (2) "ee" SPECIAL: for future use (3) cycles PY-F1B, PT-FIB/APTT, DOUBLE PT-FIB, DOUBLE PI-FIB/APTT, FIBRINOGEN-c use the field to specify the units for Fib. (mg/dl or g/1). cycles SINGLE FACTOR use the field to specify the type of factor in use and the format of the results (nigh, Low). Additional information about the test performed are specified in . Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ a) e) £) Instrumentation Laboratory, October 22, 1999 specification Starting from Rev. 09 the ATII cycle has a stored calibration with a dedicated session. The new cycle has consequently a new transmission code: “2E". For the old cycle, with in run calibration, the old transmission code “30” is mantained and will be used up to rev. 08. Starting from Rev. 09 the PCx cycle has a stored calibration with dedicated session. The new cycle has consequently 4 new transmission code: “43”. For the old cycle, with in run calibration, the old transmission code “45"is mantained and will be used up to rev. 08. Starting from Rev. 09 the HPX cycle has a stored calibration with dedicated session. The new cycle has consequently a new transmission codes: : “4c”. For the old cycle, with in run calibration, the old transmission code “ae” is mantained and will be used up to rev. 08.ACL, Step III, Phase B: RS-232 Specification «massage header> continue <# messages> 2 HEX-ASCIZ char.s: number of messages to follow 3g m2 12H= 18 in case of a PT-FIB cycle with 8 sample and SAMPLE ID option (2+8+8) a) >) ) a) e) 1 HEX-ASCIZ char. PI-FIB cycles "o" ip units= mg/dl, me off vay FIB units= g/l , INR off ve" FIB units= mg/dl, INR on wc" FIB units- g/l , INK on SINGLE FACTOR cycle "0" I1 EXTRINSIC high curve my non x "3" vir "4" IIT INTRINSIC high curve no" IX me" xT "7" IT "e" I] EXTRINSIC low curve non y van x tBr VIE "c" VIII INTRINSIC low curve "Dn Ix TE" XL "EY xIT HEPARIN cycle "oO" high curve "8" low curve PRO-IL-COMPLEX, HEPATOCOMPLEX cycle vo" INR off me" INR on FIBRINOGEN-C cycle no" IB units= mg/dl vay PIB unites g/l Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ specification continue Note: The field has a cycle dependent meaning: al b) ’ a 8) Instrumentation Laboratory, October 22, 1999 in cycles PT-FIB, PY-FIB/APTT, DOUBLE PT-FIB, DOUBLE PI-FIB/APTT, FIBRINOGEN-C the field contains the selections of the PROGS "UNITS", "CALCULATIONS". rhe units influence the format of the results . The selection INR on expresses the ratio subfield in a5 INR with an ISI value specified in the field. in cycles SINGLE FACTOR the field contains the type of factor in use and the type of its cal. curve (high/low). The selection high/low curve influences the format of the results . in HEPARIN cycle the field contains the type of its cal. curve (high/low). in cycles PRO-IL-COMPLEX and HEPATOCOMPLEX the field indicates whether the ratio subfield in is expressed as R or INR. With INR on the ISI value is specified in the field. all other cycles leave the field at zero "0". 14 aScII char.s: date of analysis " dd. mmm. yy * 9 ASCII char.s: time of analysis " bh: mmACL, Step III, Phase Br RS-232 Specification massage header> continue Notet field contain the instrument warnings occured during the cycle. In case of liquid sensor fail, the field specifies the type of error (see below) « 5 ascIr char.s: this field contains the 1si-value if ratio is expressed as INR (see rield) ratio INR Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ continue mom me specification 1 ASCII char. transmission of analytical results transmission of raw data (only for internal use) 1 HEX-ASCII char.: test characteristics "0" PT-FIB, APTT, TT in standard, PATHWAY "1" PP-FIB, APPT, TT in extended "2" DOUBLE: PT-FIB, APTT, TT, PT-FIB/APTT in stand. "3" DOUBLE: PT-FIB, APTT, TT, PT-FIB/APTT in ext. "4a" PP-FIB/APT?, TT/APTT in standard "6" DOUBLE: PT-FIB/APTT, TT/APTT in standard “B" SINGLE, ABS., SPECIAL with on-plate cal. Note: The field contains additional information about the test performed. standard/zxtended correspondes ta the selection in the PROG “ACQUISITION TIME" 1 HEX-ASCII char.: liquid sensor status "0" liquid sensor ok, reagents present "1" liquid sensor off "6" electronic fail. "7" flush out of range "eg" air out of range "9" leakage between sensors "Bn no reagent 1 "co" na reagent 2 "D" no reagent 3 "E" no reagents Notet selected. Instrumentation Laboratory, ASCII char.s: 2 ASCIT char.at The field vemaine blank if SAMPLE ID option is Opa, OAH october 22, 1999ACL, Step III, Phase B: RS-232 Specification «massage contents> + length: 28 bytes <> | | | | | see chapter 5 SY) > coordinates of calibration point my, gy £2: coefficients of calibration line (normal pool value]. (normal pool ID}... ews (test result]. sample identifier 2 ASCII char.s: ODH, DAK Note: For move details see chapter 5 test ID> + + length: 16 bytes ctest ID 2 ASCIE char.s: "00" batch no.> 12 ASCIE char.s: "KXNKXXRKERKR" 2 ASCII char.s: ODH, OAH + + length: 16 bytes 2 ASCIE char.s: "02" 12 ASCIT char.a: "KRuxHuKKERKK" 2 ASCII chars: ODH, DAK ris not for public use «mess. contents raw data> not for public use Instrumentation Laboratory, October 2: 1999ACL, Step III, Phase B: RS-232 Specification 5.1 description ‘The can contain different data types specified by tne message identifier in position 25/26: 01..18 message witn results of sample 1..18 21..38 message with SAMPLE ID of sample 1..18 50. massage with NP identifier 60. message with results of NP 70 message with coefficients of cal. line Note: the message identifier is not available on the Ist generation of the Ach. Instrumentation Laboratory, October 1999 2ACL, Step III, Phase B: RS-232 Specification The sequence of the messages is cycle dependent and is established as follows: Pr-FIB aprT 1r DOUBLE Pr-FrE DOUBLE APTT DOUBLE TT. PT-FIB/APTT ‘Tr-aprr DOUBLE Pr-FIB/APTT PATHARY - EXTR. PATHWAY - INTR. SINGLE FACTOR Ag-11T AT-III-ANALYSIS HEPARIN ANTIPLASMIN PLASMINOGEN FIBRINOGEN-C PROCHROM. PRO-IL-COMPLEX + + 18() + (18( + + 18{} + (18{ + + 18(] + (18{ + = 9( + ) + (S( + + 9( + } + (9( + + 9( + } + (3( + + 8( + ) + (8( + + 8( + ) + (2( + + 4( + “Waprr 1 + * + + 3[ + + + ) + (3( + + 3 + + + ) + (3() + + 14 (} + + 15(} + (15( + 17() + (17{} + + 15(em>) + (15(] + + 15() + (15(] + empq,r2> 4 15(ewy + (15( + 15 (} + + + + 4(<>} + (14( + 17(] + (17() + + + + A(} + (14( + 17{] + (17{) + + + + LA(} + (14{) + + + + Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ Specification L6{} + (16( message is optional and only transmitted if the SAMPLE ID option is selected (see PROG "DATA TRANSMISSION") . ‘The contents and format of the messages are explained in chapters 5.2 to 5.6. Result fields are specified with their max. length and can assume integer or floating point values. the are always transmitted without their units. Result fields with attributes (to indicate an in range/out of range) are expressed as normal letters with surrounding attributes in capital letter: cccec means the result "ccc" is associated with an attribute and will be transmitted as " 200 " in case of IN RANGE, will be transmitted as "*200*" in case of OUT OF RANGE, will be transmitted as "*---*" in case of UNDERFLOW OF DISPLAYABLE RANGE, will be transmitted as "#**+#" in case of OVERFLOW OF DISFLAYARLE RANGE. All unspecified positions in a block, actually indicated as blank, are to be ignored. Instrumentation Laboratory reserves the right to use these positions Tor internal use or future developments Instrumentation Laboratory, October 22, 1999 BLACL, Step III, Phase B: RS-232 Specification 5.2 description <¥,X> i1= coordinates of calipration point This message contains the results for one calibration point. The message igentitler speciries wnich point of the calipration line. This means, a message in a SINGLE "100_18-1 a1" has to be interpreted as: Ist cal. point with an assigned activity of 100% and a measured tine of 18.15. Values substituted by *** or --- indicate over- or underflow. A blank message (all spaces) means no data available. block | function | position of | test | units of i |_value a,b... | values 1 5 10 i Leseeeeee dee <8) > yyy xxx id SINGLE hi. bs YY Xxxx id SINGLE low as YY xxxxx id Ar-IIT 4,delta oD yyy xxaxx id ANTIPLASMIN — %,delta OD YYY ¥xxxx id PLASMINOGEN delta op YYYY 2xxx id FIBRINOGEN-C mg/dl or g/1,8 yyy xxxxx id PROCHRON delta oD YYY soe id HEPARIN U/ml, delta op yyyy xxxx id PRO-IL-COMPLEX 3,5 YYYY xxx id HEPATOCOMPLEX %, 5 YYYY xxxx id PROCLOT ays YYyy xxx id PROTEIN-S ts Notes nia" Message identifier in pos. 25, "1" Ist cal. point "22" ond cal. point "83" 3rd cal. point For 2/3 point cal. indication see . In case of 2 point cal. the third block remains blank. 32 Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ specification 5.3 description coefficients of calibration line and are signed numbers in floating point format (e. g. field is replaced by an error cade. This means, pos. 15 distinguishes 2 casest 21.778). In a 9.998 " x2 in range ») "*9,790*" 22 out range ce) 12222? " error code for 2 point cal. values substituted by *** or --- indicate over- or underflow. block | function | position | test 1 5 10 18 20 sleeeeel SINGLE PRO-IL-COMPLEX HEPATOCOMPLEX PROCLOT PROTEIN-S AT-III HEPARIN ANTIPLASNIN PLASMINOGEN FIBRINOGEN-C PROCHROM mmmmmm = gqqqqaRrrrrrR 70 (3 point cal.) mmmmmn qqqqqqi???2?7?«70~=s (2: point cal.) Note: pos. 15: "E" 2 point cal. "27222" datum for internal use. Instrumentation Laboratory, October 22, 1999 33ACL, Step III, Phase B: RS-232 Specification 5.4 description (normal pool value}... This message can contain up to 4 NP values. the message "_45.3_"60.6"f___c___60" in an intrinsic PATHWAY means: NP of factor VIII gives a time of 45.35, the time of factor Ix 60.és is out of range, factor XI yields an error, factor XII is not calibrated. The message "11.7210 26.2227 0" in an PT-FIB/APTT means: NP of PT gives a time of 11.7, the PIB has @ concentration of 210, and the NP of APTT yields a time of 36.35. Values substituted by *** or spaces) means no data available. indicate over- or underflow. A blank value (all block | function | position of | test | units of i |_value a,b... 1 | values 1 5 10 1 AaaaaABbbbB 222° 60. PT-FIB symg/dl AaaaaABDbbbe 222 60. PT-FIB sra/L aaaaal 60 TT aaaaaa, 60 APTI s AaaaaABbbbBcececC?2?—««0-«éPT-FIB/APIT. ~—s, mg/dl, = AaaaaABbbbbBCecccC??? 0 -PT-FIB/APTT. — 5, g/1,3 Aaaaaagbbbbe 60° TT-APTT 3,8 AaaaaABbbbbaccecccDddddné0 PATHWAYS 5657848 5

aAaaaaaa 60 PRO-IL-COMPLEX 5 60 HEPATOCOMPLEX = 60 PROCLOT s 60 PROTEIN-S s In case of an error the normal pool value is replaced by an error code: pos 7, 42, 13, 19 no NE all tests "A" coag. error all tests "N" not coag. Pr-FIB oT ABTT "Ee" calc. error all tests o Fn -0- PATHWAYS PRO-IL-COMPLEX "s" no sample all tests vo" not cal. PATHWAYS cw" NP out Of range PRO-IL-COMPLEX Notet ‘The

block actually remain blank for HEPATOCOMPLEX, PROCLOT, PROTEIN-S. 22222" datum for internal use with variable contents. 34 Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ specification 5.5 description normal pool ID This message contains the NP identifier, a string of 6 char.s (digits 0..9 or space), @. g. = "_51158___ __50" L 1 5 i015 20 26 Ie ole 1 | RRRERE 50. Pr-FIB rT APTT PI-FIB/APTT PT-APTT PATHWAYS . — xxRERR 50 PRO-TL-COMPLEX HEPATOCOMPLEX PROCLOT PROTEIN-S Note: The Dlock actually remains blank for PRO-IL-COMPLEX, HEPATOCOMPLEX, PROCLOT, PROTEIN-S. Instrumentation Laboratory, October 22, 1999 35ACL, Step III, Phase B: RS-232 Specification 5.6 description <> tis {test resulth... This message contains the results of one sample. the message "31.1_15_3.20*319*___04" in an PT-FIB with = "8" means: sample 4 gives a PP time of 31.38, an activity of 15%, a ratio of 3.20 INR and for Fib. a concentration of 319mg/dl with the indication out of range. values substituted by *** or spaces) means no data available. indicate over- or underflow. A blank value (all block | function | position of I test | units of L |_value a/b. 1 |_values 7 5 10 2026 leveeeeee der 1 1.17 <> Aaaaabbbbb id AT-ITI-ANALYSIS %,delta oD Aaaaaaabbbbocee id PRO-IL-COMPLEX- %,R-INR, 5 ANALYSIS Aaaaaaabbbbecec id HEPATOCOMPLEX $,R-INR,s ANALYSIS aaaagbbpaccecpadan id Pr-FIB 5,8) R-INR, mg/dl aaaaBbbbBccceDaddaD = id PT-FIB 8, 8,R-INR, g/L aaaabbbb id TT 5/R aaaabbbb id APTT rR 3.14 Aaaaabbbb id PATHWAYS as Bele AaaaA???2bbbb id SINGLE high 3,5 aAaaaaa7??7DbbD id SINGLE low as Bes <>, AaaaAbbbbb id AP-1IT delta oD Aaasabbpbb id ANTIPLASMIN —%,delta op Aaaaabbbbb id PLASMINOGEN delta op AaaaA bbb id FIBRINOGEN-C mg/dl, s Aaaaaabbbb id FIBRINOGEN-C — g/1,s Aaaaabbbbb id PROCEROM 4,delta op Aaaaaabbbbb id HEPARIN U/m,delta oD 74.20 Aaaaaaabbbbecec id PRO-TL-COMPLEX %,R-INR,s Aaaaaaabbbbccee id HEPATOCOMPLEX %,R-INR,s Aaaaaaapbbpcece id PROCLOT aR s AaaaaaA = ccce id PROTEIN-S as 36 Instrumentation Laboratory, October 22, 1999ACL, Step III, Phase B: RS~ specification im case of an error the test result is replaced by an error code: pos. 1: "A" coag. error all tests "W" not coag PI-FIB TT APTT PROTEIN-S FIBRINOGEN-C calc. error all tests -0- PATEWAYS SINGLE PRO-IL-COMPLEX HEPATOCOMPLEX PROCLOT no sample all tests pos. 25,26: "id" "ol" 1st sample "02" 2nd sample "1g" Leth sample Note: "27222" datum for internal use. Instrumentation Laboratory, October 2: , 1999 37ACL, Step III, Phase B: RS-232 Specification 5.7 description i= sample identirier This message contains the sample identifier Tor each sample. (12 digits long, block | function I position | test 1 5 do 2026 aaaaaaaaaaaa id all tests pos. 25,26: "id® ist sample 2nd sample "39" leth sample 38 Instrumentation Laboratory, october 22, right justified) 1999