SHRI RAMSWAROOP MEMORIAL UNIVERSITY

Internal Test - III

SOLUTION AND HINTS
Session : 2022-23 (Odd Sem) Semester : I
Course : M.Sc (H) Subject Code : PBS1005
Branch : BT Subject Name : Analytical Techniques of
Biotechnology
Group : 11 Names of Faculty Member : Er. Ayushi Verma
Date 01/02/2023 Duration 1Hr 15 Min Max. Marks 30 SET-I

Ans. 1 Very short type questions. [5X1=5]

a) A pH meter is a scientific instrument that measures the hydrogen-ion activity in water-based
solutions, indicating its acidity or alkalinity expressed as pH
b) What is RF Value? The Rf (retardation factor) value is the ratio of the solute's distance travelled to the
solvent's distance travelled.
c) Analyte, Mobile phase and Stationary phase
d) Blocking is a very important step of western blotting, as it prevents antibodies from binding to the
membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk diluted in
TBST to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely
available.
e) High-performance liquid chromatography
Sodium dodecyl-sulfate polyacrylamide gel electrophoresis

Ans. 2 Short Answer types Question [4X2.5=10]

a) Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used by scientists to produce a DNA
fingerprint for a bacterial isolate.
b) Ion exchange, gel permeation, affinity, HPLC
c) The key difference between 1D and 2D gel electrophoresis is that 1D gel electrophoresis separates
proteins based only on the molecular weight while 2D gel electrophoresis separates proteins based on
both iso-electric point and molecular weight
d) Ion exchangers are quickly polluted, which considerably reduces the exchange capacity. Examples of
this include pollution by micro-biology (e.g. film-forming bacteria) and pollution by suspended
matter. Another disadvantage is the relatively high operational costs for, among other things, the
regeneration fluid.
Ans. 3 Long Answer types Question [2X7.5=15]

a) Southern blot is a method used in molecular biology for the detection and quantification of a specific
DNA sequence in DNA samples.
b) as chromatography (GC) is an analytical technique used to separate and detect the chemical components
of a sample mixture to determine their presence or absence and/or quantities. These chemical components
are usually organic molecules or gases.