01/21/2004 09:48 8153511974 PRIMUS PAGE 01

2
System Keys

Unshifted System Keys

[Disp ) [Timetable

, (System on
[Load

( Status

(Pump on
) (Method
I (Sequ

) [Lomp on
I
I
)

Shifted System Keys

I
[Print I (Alph. I
[Store I ( Delete I
if,
( Ctrl ) ( In.ert I
(System off [Pump off I (Lomp off I
it

• (Shift I The (Shift I key is the blue-labeled key located on the

•"' lower-left side of the keyboard. This key accesses the

shifted functions which are also labeled blue_
·,
• When you press (Shift ), the lamp to the left of the key
comes on. You can now select a shifted function. If you
want to select a further shifted function, you must press
,

·•
(Shift! again.

••
~
, System Keys 2-1
 PRIMUS PAGE 02
01/21/2004 09:48

(System on] on the HP 1090. The HP I 1090 does a. self-test and

TIlXllS
configures the modules which aJe installed, see "Start
Up" in Chapter 1.

(Shift] (System off) Turns off the HP 1090. The cur~ent

method and any
stored methods are retained in rhemory. The meulOry is
powered by a battery.

(Pump on ) Turns on solvent delivery systerrl (SDS): lIP 1090

delivers solvent according to 'loB, %C, 'loD and FLOW. The
following is displayed:
(Pump on)
pump is On

Note ,1 l3efore you turn on .the SDS, a.lwja~s make sure,', tha,t there
1

"
ISdega.ssed solvent In each reser Olf of configllfed solvent
dannels. If the SDS is being tll ned on fOf the fi rst time
or it has been ofT for along time you must prime the
SDS:
1. Disconnect the column.
2. Fill solvent reservoirs with 2- ropanol (isopropa,nol).
:J. Degas the 2-propanol.
4. Flush each channel separately with maximum now
rate.
.5. Flush all channels si multane01 sly with maximum now
rate. 1_· _

2-2 System Keys

 01/21/2004 09:48 8153511974 PRIMUS PAGE 03

(Shift) (Pump off) Turns off SDS and displays:

Shl(t (Pump off)

(Lamp on ) Turns on detector lamp and or calibrates detector.

(Lamp on) with FPO If your HP 1090 has a built- n filter photometric
detector, (Lamp on I turns on the detector lamp and
cali brates tho:o: detector.
[Lamp on)
~ ,,'\::;;,,:: :::·,::,~::':::::':':·:i:':',:,>,,::~;;:~;:::':f"::,::':" ~:::;::, :,:::':::':':",::"':: :':::,:! ::<,::' ':,.'::':', ':':':',"::'::'::',," ,': ':,:::,:::':" ':' :" ': ::::' :', ::' ::'

··.i···~alIIP·,J:gIl.tt:.~9~.·in;.P!£g~~S§·.,·
If lamp is already on, the detector is calibrato:o:d, iio:o:e
"Calibrating the FPD."
(Lamp on I
·•.·•· .· · · · •· • ···.·····g~tO!c.t·<>F··:2iiIbfj.tfri i ; .
Calibrating the FPD

C"tb,atioll wmp,'''' cluk .",,=t meMucement. ond a

bala.nce. For dark current mleasurement, the fiHe'r who:o:o:o:l
is moved so that it blocks litht path a.nd the amount of
stray light still reaching pholtodiodes is mo:o:asll red and
subtracted from all subsequJnt measurements. vVhen a

•-
.-. balance is made, I. he eluent kbsorbance ill the flow cell is
mea.sured. During an analyJis, output iiignal is difference
between the current abwrblnce and the absorba.nee
measured at the ba.la.nee tinie.
If your HP 1090 has an autLnjector, the FPD is
- calibrated automatically berNe ani njection cycle is
iita.rted. If your HP 1090 ha~ a manual injection-valve,

System Keys 2·3

01/21/2004 09:48 8153511 974 PRIMUS PAGE 04

the FPD must be calibrated manua.lly by pressing

(Lomp on ).

(Lomp on) with DAD If your HP 1090 has a built-in or stand-alone (HP 1040)
diode-array detector connected through the Hp·IB .
communication interface, (Lomp;;n1 t\lrnS on the detector
lamp a.nd calibrates the detector.
(Lomp on)
::::,:::.:,:,:>:~,:,:,:,:,;:;,;:.:,:, . :.:",,::,/:.::,::.:':::,:":':·:·:':':'::,;,:,,;:'::"'''::::'::.:":P::;::~~:~:·:::':"'.':'~':;"".'>::::!':~!~':P~::<fo/::>i:~<:::;~<:;~ :::":i:./:':':':":':':":':;::":":i:":':i:':')::;:'>?::':: ·-t'·-:':':'!::
."/F>,,,,::/:/l'amp fgri i"tion::/J:n.. :'n :tog"re 8\:;:,:,:/::.

.--
';::.;.:':'.'::~~:'.:~~~::,:,:~::,:,:",:::::,,,:,:,:.:: "::.:,;'.:::',:,:,::.':,, ,:::, '::~::" ::, ::;':::,;::,::.... :w;;w:..~~:;;":', ,.",:,::,.r::,:::::~:,::, ,:,:'"" ,': :~,,:.':;'::::::::':':,:: ::':"::::::': ::: ::;

If lamp is already on, the detector is caJibrated, see

"Ca.Jibra,ting the DAD."
• (Lomp on I, ,.. , .
,.. ,·.···,··.·····.'i·,'·"det'ectbf ·.·2.ili~~~t·:~~g··'··········,· ...
Calibrating the DAD

Calibration comprises dark current measurement and

a balance. For dark current measurement, the shutter
is moved to block the light path and the am01.1Ut of
stra.y light still rea,ching photodiodes is measured and
subtracted from all subsequent measurements. When a
bala,nce is made, the eluent absorbance in the flow cell is
tlIc;)'sured. During an an;l,lysis, output signa,] is difference
between the current absorbance and the absorbance
measured at the ba.lance time.
If yo 1.1. ha,ve an HP 1040, pressing the (Oper"te) key on the
front panel has the same function as the (Lomp on I key on
the HP 1090 keyboard.
If your HP 1090 has an a,uto-injector, the DAD is
calibrated automa,tically before an injection cycle is
started. If your UP 1090 has a manual injection-va.lve,
the DAD must be calibrated manua.lly by pressing
(Lamp on) Or (Operate! (on the HP 1040 front panel).

2-4 System Keys

 01/21/2004 09:48 8153511974 PRIMUS PAGE 05

(Shift) (Lamp off) Turns off detector lamp and displays:

This is the display key and it can be used with the

(Method I, (X SiBn.l) and (Max Press) keys.

It is used with the (Method I key to displa.y tbe stored

methods and with the (A SiBnal) key to display tIle
.
wa,velengths of the FPD's filters .
lDliPJCi£etho<!J @)(Enter I
I-IQ:;t~tJ¢R:AtI CS 'tA,NP,J\RD FROM BP"'"
SDS-CONFIG A=1, B=1 , C=1
FLOW = 3
'I. B=20 ,C= 0

Each line of method 0 is displayed for about 2 seconds.

You can display any previously-stored method (0 to 8).
Press a,ny key to stop the listing, then move up and
down the list using the II) and 0
keys. You ca,nnot edit
any of the displayed lines.
If you try to display a method that has not been stored:
i ~ (Method L0(Enter)
-- '. , METliop:dri.$ne£iS£iint
Used with the fA Signal} key, the ~ key lists the filter
positions of the FPD.

System Keys 2·5