CONTENT

1
Introduction to
2
Detection Limit
3
Factors Affecting
4
Quantitation Limit
5
Factors Affecting
detection limit and quantitation limit (DL) (DL) (QL) (QL)

6
DL vs. QL
7
Conclusion
INTRODUCTION
Detection limit and quantitation limit are important
concepts in analytical chemistry.

They help determine the lowest concentration of

an analyte that can be reliably detected and
quantified in a sample.
 DETECTION LIMIT
(DL)
01
The detection limit is the lowest
concentration of an analyte that can be
detected with a given level of confidence.

02
It is usually defined as the concentration that
produces a signal-to-noise ratio (S/N) of 3:1.

03
This means that the signal from the analyte
must be at least three times greater than the
noise level.
FACTORS AFFECTING
DL
The detection limit is influenced by several factors
such as the instrument sensitivity, sample matrix,
and the analytical method used

The sensitivity of the instrument is a critical factor

in determining the detection limit.

The matrix effect refers to the interference caused

by other components in the sample that can
affect the analyte's signal-to-noise ratio.
QUANTITATION LIMIT
(QL)
The quantitation limit is the lowest

01 concentration of an analyte that can be

reliably quantified with a given level of
confidence.

02
It is usually defined as the concentration that
produces a signal-to-noise ratio (S/N) of 10:1.

03
This means that the signal from the analyte
must be at least ten times greater than the
noise level.
FACTORS AFFECTING
DL
Instrument sensitivity

Sample matrix

Analytical method

Quality of the standards

 DL VS. QL

01
The detection limit is the lowest concentration at which an analyte
can be detected, while the quantitation limit is the lowest
concentration at which it can be quantified.

02
The quantitation limit is usually used in regulatory guidelines to ensure
that the analytical method is sensitive enough to detect and quantify
analytes at concentrations that are relevant to the sample.

03 The quantitation limit is always higher than the detection limit.

CONCLUSION
Detection limit and quantitation limit are important concepts in
analytical chemistry that help determine the lowest concentration of
an analyte that can be reliably detected and quantified in a sample.

The detection limit is the lowest concentration at which an analyte

can be detected, while the quantitation limit is the lowest
concentration at which it can be quantified.

The quantitation limit is always higher than the detection limit and is
usually used in regulatory guidelines to ensure the analytical
method's sensitivity.