Installation Instruction

April 2008

Installation Flexor Junior series software V 4.1.0

Introduction 1

Needed parts 2

Determine the computer type 3

Backup 4

EPROM exchange on System Board 5

Change of filters 7

Installation 11

Useful information 13

Release notes 15

Host protocol 18

Document number: 3084-101-V05

 Installation Flexor Junior Series software V 4.1.0

Introduction

Dear distributor,

This kit is designed to upgrade Vitalab Junior series instruments.

This new software gives you a wide range of new features which are described in brief in the last
chapter of this instruction.

Please read the user manual to get a full understanding of this new software before the instrument
is put in to use and inform the user regarding the new possibilities of this new software release.
The software is compatible with Windows 2000® and Windows XP® operating systems.

A new host protocol based on the LIS-2A standard is introduced, the former host protocol is still
available and compatible with the former V 1.x.x software.

The following languages are supported in this software release:

Chinese
English
French
German
Italian
Portuguese
Russian
Spanish

Results of measurements performed in the former software version can not be

accessed in the new platform. If you need to access the results for regulatory
affairs you have to keep a copy of the former software on your system (described
in this upgrade instruction).

This upgrade is not possible on 3410 computers.

Migration from a former software version is not possible, reprogramming of the

application is necessary.

We always try to be as complete as possible but we can imagine that questions remain after
reading this service information. Please feel free to contact us in case of any questions.

Customer Care Department Vital Scientific B.V.

The Netherlands

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 Installation Flexor Junior Series software V 4.1.0

Needed parts

Update set 6003-107 containing:

1 x 6003-108 : Junior series software version V 4.1.0 on USB stick

1 x 6003-113 : Master board EPROM Junior V 0.2.1

1 x 3084-099 : This installation instruction

1 x 6002-900-410 : User manual English

1 x 6002-900-610 : Junior series software version V 4.1.0 on CD

User manual on CD multi-language and MSDS sheets on CD

Or update set 6003-111 containing:

1 x 3067-660 : 660 nm filter

1 x 3067-700 : 700 nm filter

1 x 6003-107 : above mentioned update kit

1 x 3820-590 : sticker (for filter positions)

And a basic tooling set to open the instrument and exchange an EPROM.

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 Installation Flexor Junior Series software V 4.1.0

Determine the computer type

The Junior has been delivered with two different computers types, the ECM-3610 and the ECM
3412.

The computer type is indicated by the third line on the monitor at the moment the computer is
started up.

Award modular BIOS V4.51PG An Stat ally

Copyright © 1981-2001, Award Software, INC

ECM-3412 BIOS Rev 2.00 (07/05/2002)

Phoenix – AwardBIOS v6.00PG. An Energy Star Ally

Copyright © 1984-2005, Phoenix Technologies, LTD

ECM-3610 Eden BIOS Rev.2.00 (3/23/2005)

A software upgrade on a 3412 computer is not possible, the reaction time of this computer is not
acceptable.

This software is intended for the ECM-3610 computer, do not install this software
on an ECM-3412 computer.

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 Installation Flexor Junior Series software V 4.1.0

Backup

Results backup
The results stored to disk in the preceding software versions are not accessible in the Error! Not a
valid link. software.
When you need to access the measurement results of the former software version, for regulatory
affairs for instance, you have to print historical results on paper or retrieve them from your LIS.

Results that are still available in the Evaluate Samples menu [F7] should also be printed.

Safety backup
To make it possible to return to the original (foregoing) software platform make a copy of the entire
Junior folder.

Select the “Junior” folder, located in the “C:\Program Files” folder, by clicking it one time
with the left mouse button.

Press the [Ctrl] + [c] key at the same time to make a copy of the “XL Analyser” folder.

Press the [Crtl] + [v] key to create a copy of the” XL Analyzer” folder (called
“Copy of Junior” folder).

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 Installation Flexor Junior Series software V 4.1.0

EPROM exchange on System Board

In case you are upgrading form software level 1.xx the Eprom of the system board has to be
upgraded to version V 0.2.1.
When the former software level is 4.xx the correct Eprom V 0.2.1 is already installed in the
instrument.

Hint: In the installation [F2] release menu you can check the version of the masterprom, If the
version is NPJ V0.2.1 you do not need to upgrade the Eprom of the system board.

Exchange of the Eprom:

Shut down the instrument and unplug the power cable.

Remove the back panel (6) by loosening the two indicated Allen head screws, to get
excess to the electronic boards.

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Installation Flexor Junior Series software V 4.1.0

Take the appropriate ESD precautions (wrist strap), and remove the EPROM
6002 l13 V x.x.x located on the on the system board (position A5)
and replace it by the EPROM 6002-113 Version V 0.2.1

Be careful that the contacts do not bend, and be sure the small indicator is facing the same
direction as the indicator on the IC holder.

Assemble the instrument and connect the power cable again.

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 Installation Flexor Junior Series software V 4.1.0

Change of filters

This chapter is only applicable for update set update set 6003-111 in this set two filters for Elitech ®
reagents (660 and 700nm) are included.

To replace the filters you have to open the instrument.

Switch off the instrument and remove the power cable from the power inlet.

Remove the front cover by lifting it and pulling it towards your self.

Remove the black front plate by removing the (4) indicated Allen head screws.

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Installation Flexor Junior Series software V 4.1.0

Open the left front door and turn the blockade 90o clock wise.

Take the left front door out by lifting it.

Now you can remove the indicated Allen head key of the left side panel and right side
panel. Take both side panels off.

Remove the sample reagent/arm from the shaft, the white ring from the wash station
and unscrew the tube bushing from the working table.

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Installation Flexor Junior Series software V 4.1.0

Now you can lift the working table, to access the optical block.

Remove the filter wheel from the optical block by loosening the screw that fixes the
Circlip on the filter wheel axis and pulling out the axis.

Remove the fixing ring from the filter wheel (eventually use a set of nose pliers to turn
the ring and pull at the same time, if you can not remove the fixing ring by hand).

Place the filter in the correct position according the following table.

Filter position Original filter wheel Updated filter wheel

(nm) (nm)
1 340 340
2 376 405
3 405 505
4 436 546
5 505 578
6 546 620
7 578 660
8 620 700

Assemble the instrument.

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Installation Flexor Junior Series software V 4.1.0

Place the new filterwheel position sticker on top of the original filterwheel sticker.
The sticker is located in the right door that covers the pipettor or at the black plate
behind the front cover.

Connect the power cable and switch the instrument on.

In the welcome screen of the new software you have the opportunity to define the
filter positions, choose the correct option.
Eventually you can alter the filter configuration in the application at a later moment
via [F5] (Special functions)  [F4] (Service)  System configuration.

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Installation Flexor Junior Series software V 4.1.0

Installation

The installation of this new software is self guiding, please notice that an upgrade from
former software versions is not possible, this is a clean install.
Take care that you have all necessary test parameters (insert sheets) available before you
start the installation of this new software.

To install the software:

Put the USB stick with software version V 4.1.0 6003-108 in a free USB port.

Click [Yes] when the above screen appears, now the installation is self guiding.
In case the above screen does not appear, open the windows explorer and start the file

“setup.exe” on the USB stick.

Click [Next >].

Click [Finish].

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 Installation Flexor Junior Series software V 4.1.0

Now the installation of the program has been finished, the application will start automatically and
the following screen will appear.

You have the opportunity to select the correct filterwheel configuration for your instrument.

You can find the correct filter settings for your instrument on the sticker located at the black plate
behind the front cover or at the sticker that is located in the right door that is covering the pipettor.
You can remove the front cover by lifting it up and pulling it towards yourself.

You have the possibility to choose between the following settings:

Make your choice and click [Ok].

If you choose custom the following screen will appear.

Enter the correct wavelength.

Click [OK].

Now the Junior application will start automatically.

Program the application with the help of the user manual, inserts sheets etc. Also perform the
necessary actions like blanking, calibrations, ISE installation etc.).
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 Installation Flexor Junior Series software V 4.1.0

Useful information

The dealer password for this software version is the same as in the former version, for more
information contact the service department.

Although we have tested this software intensively it is possible that there is a small software
imperfection that we did not notice in our investigations.

For eventual debugging we might ask you to send the Error report. (see table underneath).

In some specific situations we might ask you to send the analyzer communication host
communication or action logging.

Name Location File name

Error report C:\Documents and Settings\ Error_report_YYYYMMDD
Administrator\My Documents\ HHMMSS.zip
Analyser Error Reports\
Analyser C:\program files\Junior\debug\ Comm._log_YYYYMMDD.commlog
communication
Host communication C:\program files\Junior\debug\ Host_com_log_YYYYMMDD.txt
Action log C:\program files\Junior\debug\ Action_log_YYYYMMDD.actlog
Analyser dat file C:\Program Files\Junior\ Analprog.dat
Back_up C:\program files\Junior\debug\ Backup analyzer YYYY-MM-DD
HHMMSS.zip
Exported results C:\program files\Junior\export ExportResultsYYYYMMDDHHMMSS.t
results\ xt
Restore point C:\program RestorePointYYYYMMDD.dat
files\Junior\RestorePoints\

Y = year
M = month
D = day
H =Hour
M = minute
S = Second

To access the windows explorer when the Junior application is running, you first have to go to the
Junior Shell. You can do this by pressing the [Alt] & [Tab] at the same time.

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 Installation Flexor Junior Series software V 4.1.0

Than select the shell sign by pressing the [Tab] Key several times. When you release the
key’s you will enter the Junior shell and you can perform the needed actions.

To access the windows explorer or the Control Panel click the Explorer button.

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 Installation Flexor Junior Series software V 4.1.0

Release notes

The following list is a summarized list of changes / new features. Please refer to the user manual
for a complete overview and a description of the new possibilities.

Printouts
Presentation on printouts has been improved.
Default printouts of result will print as many patient reports as will fit on one page.
Extended report setup possibilities.
Printouts will be generated as soon as measuring on tests has been completed,
printouts may contain *INFO* messages indicating that result is not yet finalized.
Daily maintenance report printout can be reprinted at any time.

Passwords
Important parameters can be protected with a password. This password limits
access to test parameters, control parameters, calibration parameters, service
menu, system configuration, etc.
The number of available positions for test programming can be limited by use of
special password.

Calculated Results
Calculated results can be defined freely as a formula.
A calculated result can be requested for a sample, the software will automatically
request the tests needed to calculate the result.
A calculated result will be printed on default printouts.

Request samples
Order of entry fields on screen changed.
Sample types for regular samples renamed to reflect priority: Stat, Asap, and
Routine (instead of sample types: Stat, pediatric, normal, which isn’t translatable to
non-clinical use).
Order of tests can be defined by the user.
Different method for requesting controls, single screen to define control request
including possibility to define repeats.
Possibility to configure for batch oriented test requesting.

Host
New additional host protocol: CLSI LIS2-A (formerly NCCLS LIS2-A which was
formerly ASTM E1394-97). Reagent position independent, Instrument independent.
Host query support in LIS2-A host protocol.
Results can be transmitted to host as soon as they are ready. Previously results
where not transmitted until all results for a sample where ready.
Host can be connected by TCP/IP as well as Serial port.

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Installation Flexor Junior Series software V 4.1.0

Test parameters
It is possible to view/access test parameters while the instrument is active.
Layout of test parameter programming has been improved.
Additional units can be defined: Abs, dAbs, dAbs/m.
It is possible to set a repeat value 1 – 3 for a test. The instrument will use the
average absorbance measurement to calculate the concentration.
It is possible to set a duplicate difference limit on repeats. This means that repeats
must lie within a certain range of each other or the result will be flagged.
Number of decimals can be set to 4.
Concentration limits define the valid area for results. Results outside the
concentration limits will be presented as:
< x.xxx mmol/l BELOW ANALYTICAL SENSITIVITY
> y.yyy mmol/l ABOVE ASSAY RANGE
(x.xxx and y.yyy are the lower and upper concentration limit, mmol/l the selected
unit)
Predilution allows for any dilution between 1:5 and 1:100 (also 1:97, 1:60, etc.)
Sample blank can be set to ‘on request’ meaning that at time of request a sample
blank can be requested (or not) depending on the quality of the sample.
When programming reagent volumes and measuring times, a graphical feedback is
provided showing the execution of the test.
When no reagent addition is needed at R2 timing, the R3 reagent will be referred to
as the second reagent.
Results will be corrected for predilution.
Reagent volumes for R2 and R3 can be set to max. 280 ≥ µl.
For two-point measurements it is now possible to select the point before sample
addition (previously indicated by -4 for one reagent tests) in case of two and three
reagent tests. (necessary for HbA1c tests).

Calibrations
Additional curve fit routines:
Standard cubic spline – the textbook cubic spline function with draws a curve
through all the calibration points.
Smoothed cubic spline – the cubic spline function from Junior v1.2. It tries to draw
as smooth a cubic spline function as possible and may deviate from calibration
points if it results in a smoother function.
Point-to-point – A linear line is drawn from each calibration point to the next
Linear Regression – A linear line is drawn trying to stay as close to the calibration
points as possible.
4-point Logit-log, 5-point Logit-Log, 5-point exponential Logit-log – Logit-log
functions.
Possibility to define duplicate difference limits on individual calibration points.
Possibility to require operator evaluation of calibration curve.
For prediluted calibrators it is possible to use a diluent-only as 0-standard.
Factors used for calibration curve can be inspected.
Printout contains all calibration information on a single page.
Check for absorbance values outside calibration range. Values of +- 1% of lowest
and highest calibrator get flagged with outside calibration range flag.
Possible to select which standards from a multi-standard calibrator are to be used.

Controls
When programming a control with Westgard checks, only a Standard deviation
needs to be provided.

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Installation Flexor Junior Series software V 4.1.0

Profiles
Profiles can be selected from the same screen as normal tests.

Reagent positioning
User interface improvement on reagent positioning.
Free reagent positioning (A diluent may also be used for a sample blank reagent,
reagents can be freely shared between tests).

Test execution
Load/Unload request.
Batch oriented measuring.
Possibilities to configure automatic handling of accept/reject and rerun of results in
case of error flags.
Results are presented in tree-structure.
Results are presented in order defined by user.
Results can be exported to a text file in three different formats. The results can be
imported again in (for instance) Microsoft excel.
Measured results are not overwritten in case of re-measure or rerun.
Results are automatically stored to an archive file if number of results in buffer
exceeds a certain number.
Blank/Calibrator information screen shows more relevant information and allows
viewing the calibration details.

Archived results
Results are archived to archive files, including all test parameters etc. that lead to
the result.
Search function to search for results in the archive files.
Archived results can be printed on default report as well as user defined custom
report layout.

DryISE
DryISE parameters are synchronized at start up of application. Buttons ‘Sync Set
1..4’ have been removed since changes are automatically synchronized.

Handheld bar-coding
Dedicated to reading of Elitech reagent barcodes.
The expiry date is read from the reagent barcode immediately into the application
and is set to the last day of the month.
Samples can be requested and selected by scanning the barcode.

System
User interface improvements in system menu.
System configuration items moved to separate menu in service functions.
Changes log keeps track of changes to test parameters and system settings.
Restore points allow to save settings and restore to that point later.
Import/Export functions.
Extended error reporting. In case of a critical software error, all necessary files are
stored to a single error report which is saved to My Documents\Analyzer
Error Reports.
System boots immediately to Junior software.
Configurability to non-clinical use.
Quick menu is optional.
Cuvettes can be marked as ‘stained’ to extend life of the cuvette rotor. Stained
cuvettes will not be used for measurements.

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 Installation Flexor Junior Series software V 4.1.0

Host protocol

With the introduction of this new software there is a possibility to choose between two host
communication protocols.
The Vital proprietary protocol and the standardized CLSI LIS2-A protocol.

The Vital proprietary protocol is 100 % with the protocol in the former software version.

A description of both protocols is available on the documentation CD delivered with this update set.

Disclaimer: this Installation Instruction has been completed with the greatest possible care. However we cannot accept
any claim or liability based on the information in this Vital Note or information we refer to

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