DEPARTMENT OF BIOTECHNOLOGY

Batch Number
STUDENTS PROJECT PROPOSAL 2022-23

Name of the student 1.AJITHKUMAR. S

Registration number, 510419214001

Phone no 6383591076

Mail ID kumarajith6890@gmail.com

Isolation of Lipase producing gene and their application

Title of the project
in textile, medical and pharma industry

Place of work Avigen Biotech Private Limited

Internal Guide (Compulsory) Dr. R. Selvaraj

Mosa
Name of the External guide

Institute address Avigen Biotech Private Limited

Phone 8946098013
Mail
avigenbiotech@gmail.com

Duration of the project 2 Months

Attachments

1. One page write up about the project

2. Work plan
 DEPARTMENT OF BIOTECHNOLOGY

3. List of recurring and non-recurring consumables required(For internal project

only)

Signature of the student(s) Signature of the guide(s) with seal

1. Internal

2. External
 DEPARTMENT OF BIOTECHNOLOGY

ISOLATION OF LIPASE PRODUCING BACTERIAL GENE AND THEIR APPLICATION

IN TEXTILE, MEDICAL AND PHARMA INDUSTRY

AIM:

Isolation of lipase producing bacterial gene and analyse the activity of lipase
enzyme on the industrial products.

INTRODUCTION :

Lipases are industrial biocatalysts, which are involved in several novel

reactions, occurring in aqueous medium as well as non-aqueous medium. Furthermore,
they are well-known for their remarkable ability to carry out a wide variety of chemo-,
regio- and enantio-selective transformations. Lipases have been gained attention
worldwide by organic chemists due to their general ease of handling, broad substrate
tolerance, high stability towards temperatures and solvents and convenient commercial
availability.

Most of the synthetic reactions on industrial scale are carried out in organic
solvents because of the easy solubility of non-polar compounds. The effect of organic
system on their stability and activity may determine the biocatalysis pace. Because of
worldwide use of lipases, there is a need to understand the mechanisms behind the
lipase-catalyzed reactions in organic solvents.

Lipases are versatile enzymes that can be used for various kinds of
biocatalyzed reactions. Owing to their selectivity and their mild reaction conditions, they
can be often considered as more interesting than classical chemical catalysts.This
project is focused on the isolation of lipase producing genes from the microorganisms
and analysis it's applications in the textile and pharma industries.

OBJECTIVE :

Isolation of lipase producing bacteria from soil samples, production of lipase

enzyme from the bacterial species, screening and optimization of lipase enzyme activity
on fatty acids and lipid derivatives.
 DEPARTMENT OF BIOTECHNOLOGY

WORK PLAN

ACTIVITY TIMELINE (Week)

1 2 3 4 5 6

Collection of soil
samples, isolation and
identification of
bacterial species

Production of Lipase
enzyme

Partial purification of
Lipase enzyme

Screening of Lipase
enzyme

Plate assay of a Lipase

enzyme

Optimization of Lipase
enzyme at different pH,
time, temperature.
Analysis of Lipase
enzyme in industrial
applications