EXERCISE NO.

14-B
MICROBIOLOGY LABORATORY EXERCISES
ENTEROBACTERIACEAE

NAME _____________________________________________ SEC./CLASS NO. _____ RATING _____

Genus and species: Escherichia coli

SAMPLE CASE:
A 16 y/o male was brought at the ER due to crampy abdominal pain & watery diarrhea for 5x. The
patient had history of travel from the province a day prior to consult.

PE: Moderately dehydrated (sunken eyeball, poor skin turgor and dry lips & tongue)

1. What is your impression?

2. What is the pathogenesis of this infection?

3. Characterize the causative organism of this case by underlining your answer.

Morphology: Coccus Bacillus Spiral

Staining Reaction: Gram-positive Gram-negative
Motility: Motile Non-motile
Capsule: Present Absent
Spore: Present Absent
Lactose Fermentation: Yes No

4. Describe the appearance of the colonies on:

a. Eosin Methylene Blue (EMB) Agar b. McConkey Agar

5. Biochemical Characteristics: Indicate whether (+) or (-).

224-C | L A B O R A T O R Y

Answer the following question briefly.

6. Give the five groups of E. coli and their mechanisms of disease production.

GROUP MECHANISM OF DISEASE PRODUCTION

Corrected by: ____________________________________ Date: _____________