LAMPIRAN

Lampiran 1. Estimasi Volume Pengencer

Pengencer A1
V.Pengencer A1 = Volume Semen
= 0,5 ml

Pengencer A2
Jika Estimasi konsentrasi 1500 x 106 , maka :

V.Total = V.Semen segar x konsentrasi x 0,25

25 x 106

= 0,5 x 1500 x 106 x 0,25 = 7,5 ml

25 x 106

V.Pengencer A2 = V.Total – V.A1 – V.Semen

= 7,5 – 0,5 – 0,5
= 6,5 ml

44
 Lampiran 2. Data Presentase Motilitas Spermatozoa (%)
Hari ke-1
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 45 45 45 40 40 45 40 45 40 50 43,50 3,37
P1 45 40 45 40 40 45 40 45 40 50 43,00 3,50
P2 45 40 45 40 40 45 40 45 40 50 43,00 3,50

Hari ke-2
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 35 45 45 40 35 40 35 40 35 40 39,00 3,94
P1 25 35 35 35 30 35 35 35 35 40 34,00 3,94
P2 30 40 35 40 35 35 35 35 35 40 36,00 3,16

Hari ke-3
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 25 40 40 35 30 25 35 35 30 30 32,50 5,40
P1 10 35 30 30 25 25 35 25 30 30 27,50 7,17
P2 15 40 35 35 30 25 35 30 30 30 30,50 6,85

Hari ke-4
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 20 35 40 25 20 25 25 25 20 25 26,00 6,58
P1 10 30 30 20 20 20 20 25 20 25 22,00 5,87
P2 15 30 30 25 20 25 25 25 20 25 24,00 4,59

Hari ke-5
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 15 25 15 20 15 15 15 20 15 20 17,50 3,54
P1 10 10 10 20 15 15 15 15 10 15 13,50 3,37

45
P2 15 15 10 20 15 15 15 20 15 15 15,50 2,84

46
 Hari ke-6
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 10 15 15 20 15 15 15 15 15 20 15,50 2,84
P1 5 10 10 20 10 15 10 10 5 15 11,00 4,59
P2 10 15 10 20 15 15 10 15 10 15 13,50 3,37

47
 Lampiran 3. Data Presentase Viabilitas Spermatozoa (%)
Hari ke-1
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 82 84 82 74 77 77 80 73 73 74 77,20 4,39
P1 79 83 79 75 73 73 73 71 71 76 75,20 4,02
P2 75 75 82 75 72 72 75 73 72 75 74,50 3,06

Hari ke-2
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 68 69 70 67 61 65 57 64 61 61 64,30 4,24
P1 52 66 51 65 53 67 57 72 65 67 61,50 7,52
P2 55 70 65 67 53 58 56 67 65 72 62,80 6,73

Hari ke-3
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 58 66 67 68 51 59 52 58 62 63 60,40 5,91
P1 43 63 46 64 49 58 48 69 55 64 55,90 9,02
P2 53 70 67 64 47 66 48 65 60 64 60,40 8,18

Hari ke-4
U1 U2 U3 U4 U U6 U7 U8 U9 U1 Rataan SD
5 0
P 52 68 65 67 51 56 51 53 53 51 56,70 7,07
0
P 39 64 41 76 48 47 48 48 53 40 50,40 11,56
1
P 40 68 65 68 43 53 47 51 58 43 53,60 10,67
2

Hari ke-5
U1 U2 U3 U4 U U6 U7 U8 U9 U1 Rataan SD

48
 5 0
P 55 64 59 66 40 47 47 45 45 47 51,50 8,90
0
P 35 47 37 74 32 42 43 44 37 43 43,40 11,71
1
P 34 67 58 68 45 42 42 47 45 44 49,20 11,30
2

49
 Hari ke-6
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 35 42 43 49 36 32 59 37 48 47 42,80 8,19
P1 29 39 30 38 49 39 32 20 27 41 34,40 8,36
P2 26 55 36 42 37 37 31 30 33 52 37,90 9,36

50
 Lampiran 4. Data Presentase Abnormalitas Spermatozoa
(%)
Hari ke-1
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 8 8 7 7 8 7 8 7 8 7 7,50 0,53
P1 10 9 9 9 9 8 9 8 8 8 8,70 0,67
P2 8 8 8 9 8 9 8 8 8 8 8,20 0,42

Hari ke-2
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 10 8 12 8 5 6 6 9 7 8 7,90 2,08
P1 9 6 9 4 6 6 7 6 8 7 6,80 1,55
P2 10 9 8 7 6 8 6 7 8 7 7,60 1,26

Hari ke-3
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 8 8 8 7 7 7 7 7 6 7 7,20 0,63
P1 8 8 8 8 7 7 7 8 9 7 7,70 0,67
P2 9 8 7 6 7 9 7 7 8 7 7,50 0,97

Hari ke-4
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 8 9 10 8 8 9 8 8 9 8 8,50 0,71
P1 10 10 8 9 8 7 7 8 9 10 8,60 1,17
P2 9 9 9 9 8 8 9 9 9 8 8,70 0,48

Hari ke-5
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 9 7 10 8 8 7 8 8 8 9 8,20 0,92

51
P1 9 10 8 9 8 8 7 7 9 7 8,20 1,03
P2 9 9 9 9 9 9 9 9 9 8 8,90 0,32

52
 Hari ke-6
U U2 U3 U U5 U6 U U8 U U10 Rataan SD
1 4 7 9
P0 8 8 8 9 8 8 9 9 9 8 8,40 0,52
P1 10 9 9 9 9 8 9 11 9 8 9,10 0,88
P2 9 8 9 9 8 8 9 10 8 8 8,60 0,70

53
Lampiran 5. Analisis Statistik Motilitas
Analisis statistik motilitas semen hari ke-1

Perlakuan
Ulangan Total
P0 P1 P2
1 45 45 45 135
2 45 40 40 125
3 45 45 45 135
4 40 40 40 120
5 40 40 40 120
6 45 45 45 135
7 40 40 40 120
8 45 45 45 135
9 40 40 40 120
10 50 50 50 150
Total 435 430 430 1295
Rataan 43,50 43,00 43,00
SD 3,37 3,50 3,50  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (1295)2 / (3 x 10)
= 55900,83
JK Total = Σti =1Σrj =1Υіј2 – FK
= 452 + 452 + ...,, + 502 – 55900,83
= 324,17

54
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 1352 + 1252 + ...,, + 1502 / 3 – 55900,83
= 307,50
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 4352 + 4302 + 4302 / 10 – 55900,83
= 1,67
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 15,00

55
Tabel anova motilitas semen hari ke-1
F
Fhitun F
SK db JK KT 0,0
g 0,01
5
Kelompo 2,4
9 307,50 34,17 41,00 3,60
k 6
3,5
Perlakuan 2 1,67 0,83 1,00 6,01
5
Galat 18 15,00 0,83
1416,6
Total 29        
7

56
Lampiran 5. Analisis Statistik Motilitas
Analisis statistik motilitas semen hari ke-2

Perlakuan
Ulangan Total
P0 P1 P2
1 35 25 30 90
2 45 35 40 120
3 45 35 35 115
4 40 35 40 115
5 35 30 35 100
6 40 35 35 110
7 35 35 35 105
8 40 35 35 110
9 35 35 35 105
10 40 40 40 120
Total 390 340 360 1090
Rataan 39,00 34,00 36,00
SD 3,94 3,94 3,16  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (1090)2 / (3 x 10)
= 39603,33
JK Total = Σti =1Σrj =1Υіј2 – FK
= 352 + 252 + ...,, + 402 – 39603,33
= 496,67

57
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 902 + 1202 + ...,, + 1202 / 3 – 39603,33
= 263,33
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 3902 + 3402 + 3602 / 10 – 39603,33
= 126,67
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 106,67

58
Tabel anova motilitas semen hari ke-2
F F
SK db JK KT Fhitung
0,05 0,01
Kelompo 263,3
9 29,26 4,94 2,46 3,60
k 3
126,6
Perlakuan 2 63,33 10,69 3,55 6,01
7
106,6
Galat 18 5,93
7
496,6
Total 29        
7

Uji Jarak Berganda Duncan

SE = √(KT Galat / r)
= √(5,93 / 10)
= 0,77
Tabel Kritis UJBD

  2 3
SE 0,77 0,77
Nilai di Tabel Duncan 4,071 4,246
Hasil Hitung 3,13 3,27

Tabel Notasi Perlakuan

Perlakuan Rataan     Notasi

P1 34,00 a a

59
 P2 36,00 a b ab
P0 39,00   b b

Lampiran 5. Analisis Statistik Motilitas

Analisis statistik motilitas semen hari ke-3

Perlakuan
Ulangan Total
P0 P1 P2
1 25 10 15 50
2 40 35 40 115
3 40 30 35 105
4 35 30 35 100
5 30 25 30 85
6 25 25 25 75
7 35 35 35 105
8 35 25 30 90
9 30 30 30 90
10 30 30 30 90
Total 325 275 305 905
Rataan 32,50 27,50 30,50
SD 5,40 7,17 6,85  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (905)2 / (3 x 10)
= 27300,83
JK Total = Σti =1Σrj =1Υіј2 – FK

60
 = 252 + 102 + ...,, + 302 – 27300,83
= 1274,17
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 502 + 1152 + ...,, + 902 / 3 – 27300,83
= 1007,50
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 3252 + 2752 + 3052 / 10 – 27300,83
= 126,67
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 140,00

61
Tabel anova motilitas semen hari ke-3
F F
SK db JK KT Fhitung
0,05 0,01
Kelompo 1007,5
9 111,94 14,39 2,46 3,60
k 0
Perlakuan 2 126,67 63,33 8,14 3,55 6,01
Galat 18 140,00 7,78
1274,1
Total 29        
7

Uji Jarak Berganda Duncan

SE = √(KT Galat / r)
= √(7,78 / 10)
= 0,88
Tabel Kritis UJBD

  2 3
SE 0,88 0,88
Nilai di Tabel Duncan 4,071 4,246
Hasil Hitung 3,59 3,74

Tabel Notasi Perlakuan

Perlakuan Rataan     Notasi

P1 27,50 a a
P2 30,50 a b ab
P0 32,50   b b

62
Lampiran 5. Analisis Statistik Motilitas
Analisis statistik motilitas semen hari ke-4

Perlakuan
Ulangan Total
P0 P1 P2
1 20 10 15 45
2 35 30 30 95
3 40 30 30 100
4 25 20 25 70
5 20 20 20 60
6 25 20 25 70
7 25 20 25 70
8 25 25 25 75
9 20 20 20 60
10 25 25 25 75
Total 260 220 240 720
Rataan 26,00 22,00 24,00
SD 6,58 5,87 4,59  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (720)2 / (3 x 10)
= 17280,00
JK Total = Σti =1Σrj =1Υіј2 – FK
= 202 + 102 + ...,, + 252 – 17280,00

63
 = 970,00
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 452 + 952 + ...,, + 752 / 3 – 17280,00
= 786,67
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 2602 + 2202 + 2402 / 10 – 17280,00
= 80,00
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 103,33

64
Tabel anova motilitas semen hari ke-4
Fhitun F F
SK db JK KT
g 0,05 0,01
Kelompok 9 1007,50 111,94 15,23 2,46 3,60
Perlakuan 2 126,67 63,33 6,97 3,55 6,01
Galat 18 140,00 7,78
Total 29 1274,17        

Uji Jarak Berganda Duncan

SE = √(KT Galat / r)
= √(7,78 / 10)
= 0,76
Tabel Kritis UJBD

  2 3
SE 0,76 0,76
Nilai di Tabel Duncan 4,071 4,246
Hasil Hitung 3,08 3,22

Tabel Notasi Perlakuan

Perlakuan Rataan     Notasi

P1 22,00 a a
P2 24,00 a b ab
P0 26,00   b b

65
Lampiran 5. Analisis Statistik Motilitas
Analisis statistik motilitas semen hari ke-5

Perlakuan
Ulangan Total
P0 P1 P2
1 15 10 15 40
2 25 10 15 50
3 15 10 10 35
4 20 20 20 60
5 15 15 15 45
6 15 15 15 45
7 15 15 15 45
8 20 15 20 55
9 15 10 15 40
10 20 15 15 50
Total 175 135 155 465
Rataan 17,50 13,50 15,50
SD 3,54 3,37 2,84  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (465)2 / (3 x 10)
= 7207,50
JK Total = Σti =1Σrj =1Υіј2 – FK
= 152 + 102 + ...,, + 152 – 7207,50
= 367,50

66
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 402 + 502 + ...,, + 502 / 3 – 7207,50
= 167,50
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 1752 + 1352 + 1552 / 10 – 7207,50
= 80,00
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 120,00

67
Tabel anova motilitas semen hari ke-5
Fhitun F F
SK db JK KT
g 0,05 0,01
Kelompo
9 167,50 18,61 2,79 2,46 3,60
k
Perlakuan 2 80,00 40,00 6,00 3,55 6,01
Galat 18 120,00 6,67
Total 29 367,50        

Uji Jarak Berganda Duncan

SE = √(KT Galat / r)
= √(6,67 / 10)
= 0,82
Tabel Kritis UJBD

  2 3
SE 0,82 0,82
Nilai di Tabel Duncan 2,971 3,117
Hasil Hitung 2,43 2,55

Tabel Notasi Perlakuan

Perlakuan Rataan     Notasi

P1 13,50 a a
P2 15,50 a b ab
P0 17,50   b b

68
Lampiran 5. Analisis Statistik Motilitas
Analisis statistik motilitas semen hari ke-6

Perlakuan
Ulangan Total
P0 P1 P2
1 10 5 10 25
2 15 10 15 40
3 15 10 10 35
4 20 20 20 60
5 15 10 15 40
6 15 15 15 45
7 15 10 10 35
8 15 10 15 40
9 15 5 10 30
10 20 15 15 50
Total 155 110 135 400
Rataan 15,50 11,00 13,50
SD 2,84 4,59 3,37  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (400)2 / (3 x 10)
= 5333,33
JK Total = Σti =1Σrj =1Υіј2 – FK
= 102 + 52 + ...,, + 152 – 5333,33
= 466,67

69
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 252 + 402 + ...,, + 502 / 3 – 5333,33
= 300,00
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 1552 + 1102 + 1352 / 10 – 5333,33
= 101,67
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 65,00

70
Tabel anova motilitas semen hari ke-6
F
Fhitun F
SK db JK KT 0,0
g 0,01
5
Kelompo 300,0 33,3 2,4
9 9,23 3,60
k 0 3 6
101,6 50,8 3,5
Perlakuan 2 14,08 6,01
7 3 5
Galat 18 65,00 3,61
466,6
Total 29        
7

Uji Jarak Berganda Duncan

SE = √(KT Galat / r)
= √(3,61 / 10)
= 0,60
Tabel Kritis UJBD

  2 3
SE 0,60 0,60
Nilai di Tabel Duncan 4,071 4,246
Hasil Hitung 2,45 2,55

Tabel Notasi Perlakuan

Perlakuan Rataan     Notasi

P1 11,00 a a

71
 P2 13,50 b b
P0 15,50   b b

Lampiran 6. Analisis Statistik Viabilitas

Analisis statistik viabilitas semen hari ke-1

Perlakuan
Ulangan Total
P0 P1 P2
1 82 79 75 236
2 84 83 75 242
3 82 79 82 243
4 74 75 75 224
5 77 73 72 222
6 73 72 71 216
7 80 73 75 228
8 73 71 73 217
9 73 71 72 216
10 74 76 75 225
Total 772 752 745 2269
Rataan 77,20 75,20 74,50
SD 4,39 4,02 3,06  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (2269)2 / (3 x 10)
= 171612,03
JK Total = Σti =1Σrj =1Υіј2 – FK

72
 = 822 + 792 + ...,, + 752 – 171612,03
= 442,97
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 2362 + 2422 + ...,, + 2252 / 3 – 171612,03
= 314,30
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 7722 + 7522 + 7452 / 10 – 171612,03
= 39,27
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 89,40

73
Tabel anova viabilitas semen hari ke-1
F F
d Fhitun
SK JK KT 0,0 0,0
b g
5 1
314,3 34,9 2,4 3,6
Kelompok 9 7,03
0 2 6 0
19,6 3,5 6,0
Perlakuan 2 39,27 3,95
3 5 1
1
Galat 89,40 4,97
8
2 442,9
Total        
9 7

Uji Jarak Berganda Duncan

SE = √(KT Galat / r)
= √(4,97 / 10)
= 0,70
Tabel Kritis UJBD

  2 3
SE 0,70 0,70
Nilai di Tabel Duncan 2,971 3,117
Hasil Hitung 2,09 2,20

Tabel Notasi Perlakuan

Perlakua
Rataan     Notasi
n

74
 P2 74,50 a a
P1 75,20 a b ab
P0 77,20   b b

Lampiran 6. Analisis Statistik Viabilitas

Analisis statistik viabilitas semen hari ke-2

Perlakuan
Ulangan Total
P0 P1 P2
1 68 52 55 175
2 69 66 70 205
3 70 51 65 186
4 67 65 67 199
5 61 53 53 167
6 65 67 58 190
7 57 57 56 170
8 64 72 67 203
9 61 65 65 191
10 61 67 72 200
Total 643 615 628 1886
Rataan 64,30 61,50 62,80
SD 4,24 7,52 6,73  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (1886)2 / (3 x 10)
= 118566,53

75
JK Total = Σti =1Σrj =1Υіј2 – FK
= 682 + 522 + ...,, + 722 – 118566,53
= 1117,47
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 1752 + 2052 + ...,, + 2002 / 3 – 118566,53
= 575,47
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 6432 + 6152 + 6282 / 10 – 118566,53
= 39,27
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 502,73

76
Tabel anova viabilitas semen hari ke-2
Fhitun F F
SK db JK KT
g 0,05 0,01
Kelompok 9 575,47 63,94 2,29 2,46 3,60
Perlakuan 2 39,27 19,63 0,70 3,55 6,01
Galat 18 502,73 27,93
Total 29 1117,47        

77
Lampiran 6. Analisis Statistik Viabilitas
Analisis statistik viabilitas semen hari ke-3

Perlakuan
Ulangan Total
P0 P1 P2
1 58 43 53 154
2 66 63 70 199
3 67 46 67 180
4 68 64 64 196
5 51 49 47 147
6 59 58 66 183
7 52 48 48 148
8 58 69 65 192
9 62 55 60 177
10 63 64 64 191
Total 604 559 604 1767
Rataan 60,40 55,90 60,40
SD 5,91 9,02 8,18  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (1767)2 / (3 x 10)
= 104076,30
JK Total = Σti =1Σrj =1Υіј2 – FK
= 582 + 432 + ...,, + 642 – 104076,30
= 1784,70

78
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 1542 + 1992 + ...,, + 1912 / 3 – 104076,30
= 1193,37
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 6042 + 5592 + 6042 / 10 – 104076,30
= 135,00
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 456,33

79
Tabel anova viabilitas semen hari ke-3
Fhitun F F
SK db JK KT
g 0,05 0,01
Kelompok 9 1193,37 132,60 5,23 2,46 3,60
Perlakuan 2 135,00 67,50 2,66 3,55 6,01
Galat 18 456,33 25,35
Total 29 1784,70        

80
Lampiran 6. Analisis Statistik Viabilitas
Analisis statistik viabilitas semen hari ke-4

Perlakuan
Ulangan Total
P0 P1 P2
1 52 39 40 131
2 68 64 68 200
3 65 41 65 171
4 67 76 68 211
5 51 48 43 142
6 56 47 53 156
7 51 48 47 146
8 53 48 51 152
9 53 53 58 164
10 51 40 43 134
Total 567 504 536 1607
Rataan 56,70 50,40 53,60
SD 7,07 11,56 10,67  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (1607)2 / (3 x 10)
= 86081,63
JK Total = Σti =1Σrj =1Υіј2 – FK
= 522 + 392 + ...,, + 432 – 86081,63
= 2875,37

81
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 1312 + 2002 + ...,, + 1342 / 3 – 86081,63
= 2150,03
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 5672 + 5042 + 5362 / 10 – 86081,63
= 198,47
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 526,87

82
Tabel anova viabilitas semen hari ke-4
F F
Fhitun
SK db JK KT 0,0 0,0
g
5 1
Kelompo 2150,0 238,8 2,4 3,6
9 8,16
k 3 9 6 0
3,5 6,0
Perlakuan 2 198,47 99,23 3,39
5 1
Galat 18 526,87 29,27
2875,3
Total 29        
7

83
Lampiran 6. Analisis Statistik Viabilitas
Analisis statistik viabilitas semen hari ke-5

Perlakuan
Ulangan Total
P0 P1 P2
1 55 35 34 124
2 64 47 67 178
3 59 37 58 154
4 66 74 68 208
5 40 32 45 117
6 47 42 42 131
7 47 43 42 132
8 45 44 47 136
9 45 37 45 127
10 47 43 44 134
Total 515 434 492 1441
Rataan 51,50 43,40 49,20
SD 8,90 11,71 11,30  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (1441)2 / (3 x 10)
= 69216,03
JK Total = Σti =1Σrj =1Υіј2 – FK
= 552 + 352 + ...,, + 452 – 69216,03
= 3444,97

84
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 1242 + 1782 + ...,, + 1342 / 3 – 69216,03
= 2415,63
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 5152 + 4342 + 4922 / 10 – 69216,03
= 348,47
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 680,87

85
Tabel anova viabilitas semen hari ke-5
F F
d Fhitun
SK JK KT 0,0 0,0
b g
5 1
Kelompo 2415,6 268,4 2,4 3,6
9 7,10
k 3 0 6 0
174,2 3,5 6,0
Perlakuan 2 348,47 4,61
3 5 1
1
Galat 680,87 37,83
8
2 3444,9
Total        
9 7

Uji Jarak Berganda Duncan

SE = √(KT Galat / r)
= √(37,87 / 10)
= 1,94
Tabel Kritis UJBD

  2 3
SE 1,94 1,94
Nilai di Tabel Duncan 2,971 3,117
Hasil Hitung 5,78 6,06

Tabel Notasi Perlakuan

Perlakuan Rataan   Notasi

86
 P1 43,40 a a
P2 49,20 a b ab
P0 51,50 b b

Lampiran 6. Analisis Statistik Viabilitas

Analisis statistik viabilitas semen hari ke-6

Perlakuan
Ulangan Total
P0 P1 P2
1 35 29 26 90
2 42 39 55 136
3 43 30 36 109
4 49 38 42 129
5 36 49 37 122
6 32 39 37 108
7 59 32 31 122
8 37 20 30 87
9 48 27 33 108
10 47 41 52 140
Total 428 344 379 1151
34,4
Rataan 42,80 37,90
0
SD 8,19 8,36 9,36  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (1151)2 / (3 x 10)
= 44160,03

87
JK Total = Σti =1Σrj =1Υіј2 – FK
= 352 + 292 + ...,, + 522 – 44160,03
= 2376,97
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 902 + 1362 + ...,, + 1402 / 3 – 44160,03
= 967,63
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 4282 + 3442 + 3792 / 10 – 44160,03
= 356,07
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 1053,27

88
Tabel anova viabilitas semen hari ke-6
F F
Fhitun
SK db JK KT 0,0 0,0
g
5 1
Kelompo 107,5 2,4 3,6
9 967,63 1,84
k 1 6 0
Perlakua 178,0 3,5 6,0
2 356,07 3,04
n 3 5 1
1053,2
Galat 18 58,51
7
2376,9
Total 29        
7

89
Lampiran 7. Analisis Statistik Abnormalitas
Analisis statistik abnormalitas semen hari ke-1

Perlakuan
Ulangan Total
P0 P1 P2
1 8 10 8 26
2 8 9 8 25
3 7 9 8 24
4 7 9 9 25
5 8 9 8 25
6 7 8 9 24
7 8 9 8 25
8 7 8 8 23
9 8 8 8 24
10 7 8 8 23
Total 75 87 82 244
Rataan 7,50 8,70 8,20
SD 0,53 0,67 0,42  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (244)2 / (3 x 10)
= 1984,53
JK Total = Σti =1Σrj =1Υіј2 – FK
= 82 + 102 + ...,, + 82 – 1984,53
= 15,47

90
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 262 + 252 + ...,, + 232 / 3 – 1984,53
= 2,80
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 752 + 872 + 822 / 10 – 1984,53
= 7,27
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 5,40

91
Tabel anova abnormalitas semen hari ke-1
Fhitun F F
SK db JK KT
g 0,05 0,01
Kelompo
9 2,80 0,31 1,04 2,46 3,60
k
Perlakuan 2 7,27 3,63 12,11 3,55 6,01
Galat 18 5,40 0,30
Total 29 15,47        

Uji Jarak Berganda Duncan

SE = √(KT Galat / r)
= √(0,30/ 10)
= 0,17
Tabel Kritis UJBD

  2 3
SE 0,17 0,17
Nilai di Tabel Duncan 4,071 4,246
Hasil Hitung 0,71 0,74

Tabel Notasi Perlakuan

Perlakuan Rataan     Notasi

P0 7,50 a a
P2 8,20 a b ab
P1 8,70   b b

92
Lampiran 7. Analisis Statistik Abnormalitas
Analisis statistik abnormalitas semen hari ke-2

Perlakuan
Ulangan Total
P0 P1 P2
1 10 9 10 29
2 8 6 9 23
3 12 9 8 29
4 8 4 7 19
5 5 6 6 17
6 6 6 8 20
7 6 7 6 19
8 9 6 7 22
9 7 8 8 23
10 8 7 7 22
Total 79 68 76 223
6,8
Rataan 7,90 7,60
0
1,5
SD 2,08 1,26  
5

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (223)2 / (3 x 10)
= 1657,63
JK Total = Σti =1Σrj =1Υіј2 – FK
= 102 + 92 + ...,, + 72 – 1657,63

93
 = 81,37
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 292 + 232 + ...,, + 222 / 3 – 1657,63
= 48,70
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 792 + 682 + 762 / 10 – 1657,63
= 6,47
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 26,20

94
Tabel anova abnormalitas semen hari ke-2
Fhitun F F
SK db JK KT
g 0,05 0,01
Kelompo 48,7
9 5,41 3,72 2,46 3,60
k 0
Perlakuan 2 6,47 3,23 2,22 3,55 6,01
26,2
Galat 18 1,46
0
81,3
Total 29        
7

95
Lampiran 7. Analisis Statistik Abnormalitas
Analisis statistik abnormalitas semen hari ke-3

Perlakuan
Ulangan Total
P0 P1 P2
1 8 8 9 25
2 8 8 8 24
3 8 8 7 23
4 7 8 6 21
5 7 7 7 21
6 7 7 9 23
7 7 7 7 21
8 7 8 7 22
9 6 9 8 23
10 7 7 7 21
Total 72 77 75 224
Rataan 7,20 7,70 7,50
SD 0,63 0,67 0,97  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (224)2 / (3 x 10)
= 1672,53
JK Total = Σti =1Σrj =1Υіј2 – FK
= 82 + 82 + ...,, + 72 – 1672,53
= 17,47

96
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 252 + 242 + ...,, + 212 / 3 – 1672,53
= 6,13
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 722 + 772 + 752 / 10 – 1672,53
= 1,27
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 10,07

97
Tabel anova abnormalitas semen hari ke-3
F
Fhitun F
SK db JK KT 0,0
g 0,01
5
2,4
Kelompok 9 6,13 0,68 1,22 3,60
6
3,5
Perlakuan 2 1,27 0,63 1,13 6,01
5
10,0
Galat 18 0,56
7
17,4
Total 29        
7

98
Lampiran 7. Analisis Statistik Abnormalitas
Analisis statistik abnormalitas semen hari ke-4

Perlakuan
Ulangan Total
P0 P1 P2
1 8 10 9 27
2 9 10 9 28
3 10 8 9 27
4 8 9 9 26
5 8 8 8 24
6 9 7 8 24
7 8 7 9 24
8 8 8 9 25
9 9 9 9 27
10 8 10 8 26
Total 85 86 87 258
Rataan 8,50 8,60 8,70
SD 0,71 1,17 0,48  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (258)2 / (3 x 10)
= 2218,80
JK Total = Σti =1Σrj =1Υіј2 – FK
= 82 + 102 + ...,, + 82 – 2218,80
= 19,20

99
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 272 + 282 + ...,, + 262 / 3 – 2218,80
= 6,53
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 852 + 862 + 872 / 10 – 2218,80
= 0,20
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 12,47

100
Tabel anova abnormalitas semen hari ke-4
F F
SK db JK KT Fhitung
0,05 0,01
Kelompo
9 6,53 0,73 0,70 2,46 3,60
k
Perlakuan 2 0,20 0,10 2,19 3,55 6,01
Galat 18 12,47 0,69
Total 29 19,20        

101
Lampiran 7. Analisis Statistik Abnormalitas
Analisis statistik abnormalitas semen hari ke-5

Perlakuan
Ulangan Total
P0 P1 P2
1 9 9 9 27
2 7 10 9 26
3 10 8 9 27
4 8 9 9 26
5 8 8 9 25
6 7 8 9 24
7 8 7 9 24
8 8 7 9 24
9 8 9 9 26
10 9 7 8 24
Total 82 82 89 253
8,2
Rataan 8,20 8,90
0
1,0
SD 0,92 0,32  
3

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (253)2 / (3 x 10)
= 2133,63
JK Total = Σti =1Σrj =1Υіј2 – FK
= 92 + 92 + ...,, + 82 – 2133,63

102
 = 21,37
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 272 + 262 + ...,, + 242 / 3 – 2133,63
= 4,70
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 822 + 822 + 892 / 10 – 2133,63
= 3,27
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 13,40

103
Tabel anova abnormalitas semen hari ke-5
d Fhitun F F
SK JK KT
b g 0,05 0,01
0,5
Kelompok 9 4,70 0,70 2,46 3,60
2
1,6
Perlakuan 2 3,27 2,19 3,55 6,01
3
1 0,7
Galat 13,40
8 4
2
Total 21,37        
9

104
Lampiran 7. Analisis Statistik Abnormalitas
Analisis statistik abnormalitas semen hari ke-6

Perlakuan
Ulangan Total
P0 P1 P2
1 8 10 9 27
2 8 9 8 25
3 8 9 9 26
4 9 9 9 27
5 8 9 8 25
6 8 8 8 24
7 9 9 9 27
8 9 11 10 30
9 9 9 8 26
10 8 8 8 24
Total 84 91 86 261
Rataan 8,40 9,10 8,60
SD 0,52 0,88 0,70  

FK = (Σti =1Σrj =1Υіј)2 /(t x r)

= (261)2 / (3 x 10)
= 2270,70
JK Total = Σti =1Σrj =1Υіј2 – FK
= 82 + 102 + ...,, + 82 – 2270,70
= 16,30

105
JK Kelompok = Σti =1(Σrj =1Υіј2) / t – FK
= 272 + 252 + ...,, + 242 / 3 – 2270,70
= 9,63
JK Perlakuan = Σti =1(Σrj =1Υіј2) / r – FK
= 842 + 912 + 862 / 10 – 2270,70
= 2,60
JK Galat = JK Total – JK Kelompok – JK
Perlakuan
= 4,07

106
Tabel anova abnormalitas semen hari ke-6
Fhitun F F
SK db JK KT
g 0,05 0,01
Kelompo
9 9,63 1,07 4,74 2,46 3,60
k
Perlakuan 2 2,60 1,30 5,75 3,55 6,01
Galat 18 4,07 0,23
Total 29 16,30        

Uji Jarak Berganda Duncan

SE = √(KT Galat / r)
= √(0,23/ 10)
= 0,15
Tabel Kritis UJBD

  2 3
SE 0,15 0,15
Nilai di Tabel Duncan 2,971 3,117
Hasil Hitung 0,45 0,47

Tabel Notasi Perlakuan

Perlakuan Rataan     Notasi

P0 8,40 a a
P2 8,60 a a
P1 9,10   b b

107
Lampiran 8. Dokumentasi

Kelapa Merah Sapi Limousin Antibiotik

Telur Buffer Inaktifasi

Homogenisasi Pengecekan pH Sentrifuge

108