Tittle Author Year Method

Regenerasi Kalus Sambung Sitti Fatimah Syahid, Lusia 2021 Media dasar yang digunakan adalah
Nyawa {Gynura Seti Murashige dan Skoog (MS) yang
procumbens (Lour.) Merr.} diperkaya vitamin dari grup B.
In Vitro Sebagai sumber energi ditambahkan
Callus Regeneration of sucrosa sebanyak 30 g/l dan media
Gynura procumbens (Lour.)
dibuat padat dengan penambahan
Merr. In Vitro
bakto agar sebanyak 8 g/l. pH media
diatur sampai 5,8 dengan
penambahan NaOH atau HCl. The
best media treatment for callus
induction was 0.5 mg L -1 2,4-D +
0.5 mg L -1 BA. This treatment
produced friable callus structure, no
roots and yellowish white.
Effect of Plant Growth Amin Nurokhman1, Hanik 2019 Leaf explant was cut ± 2 cm2 while
Regulator and Explant Faizah, Sugiharto, Edy Setiti internode, stem nodes and petiole
Types on in vitro Callus Wida Utami1 and Yosephine explants were cut 0.5-1 cm and
Induction of Gynura Sri Wulan Manuhara inoculated in MS medium14
procumbens supplemented with 30 g/L sucrose, 8
(Lour.) Merr g/L agar and the results of this study
indicated that the treatment of 0.5
mg/L NAA and 0.5 mg/L BAP on
the petiole explants was the best
combination of plant growth
regulators to produce the highest
callus fresh and dry weights (1478.1
mg and 40.0 mg respectively).
Callus derived from leaf, petiole and
internode explants was friable and
compact in texture while node
explantderived callus was compact
in texture. Cultures were incubated
at 25±3°C under light of 320 lux for
28 days. Fresh weight, dry weight
and morphology of callus were
observed at the end of cultivation
High efficiency Li-Sha Liu, Ren Li, Yongqin 2011 The leaves were cut into 1 cm2
regeneration and genetic Zhao, Chang-Long Wen, pieces and were used as explants.
stability analysis of Shuxin Ren and Yang-Dong Ten explants were inoculated in a 90
somatic clones of Gynura Guo mm Petri dishes containing 25 cm3
bicolor DC. solidified medium. Leaf explants
were placed with the adaxial
surface towards the medium and
then incubated in a growth chamber
at 26 ± 2°C with a photoperiod of 14
h and light intensity of 40 mol m-2
s-1 provided by fluorescent tubes.
The best callus induction of plant
regeneration was obtained in
combination of 2,4-D at 2.0
mg/l and BA at 0.5 mg/l, and the
frequency of regenerating explants
was 78.3%. After four-week callus
induction, the calli were transferred
to shoot induction medium
containing TDZ at 0.5 mg/l and
AgNO3 at 4 mg/l. The calli were
subcultured every four weeks. The
number of explants with shoot buds
was scored after 15 weeks culture
and the adventitious shoots formed
per explant were counted.