Nama : Winada Reyhan

NIM : 215130101111037
Kelas : 2021C
No Absen : 23
RESUME KULIAH 3IN1 TRIB
ASSITED REPRODUCTIVE TECHNOLOGIES IN CATTLE AND PIGS

Natural Mating In Cattle

No breeding season
Polyestrus
Pregnancy of 9 month
Oestrus of 18 hours
Ovulation of mature oocytes 12 hours after the end of estrus
Mono-ovulatory species
Malting takes a second or 2
One calf per year

AI in cattle : semen
Mostly frozen-thawed

Benefits of artificial insemination

Increases number of offspring from top sires
Faster genetic progress
Shipping of frozen semen from top sires throughout the world
Control of venereal diseases

Superovulation and embryo transfer

Selection of the donor cow
Superovulation of donor cow
Insemination of donor cow
Flushing the embryos
Evaluating the embryos
Selection and preparation of recipient females
Transfer of the embryos

Superovulation
Release of multiple eggs at single heat
85% of donors will produce on average 5 embryos
Prostaglandin injection given causes the donor to come into estrus within 48-60 h
Staging and grading of embryos
Stage 1 (1 cell)
Stage 2 (2 cell)
Stage 2 (4 to 8 cell)
Stage 2 (16 cell)
Stage 3 (early morula)
Stage 4 (Compact morula)

Selection of recipient and transfer

Young dairy cows
Healthy
Calving ease

Benefits of embryo transfer

Increases number of offspring from valuable females
Faster genetic progress
Increase frequency of desired combinations

Advantages of ovum pick-up

Minimally invasive
Highly repeatable

Collection of ovaries in abattoir

In thermocontainer
No addition of extra fluid
No temperature control
Ovaries are tepid upon arrival
Removal of connective tissue

Preparation of semen for IVF

Selection of good quality sperm
Determine concentration
Capacitation conditions

Oocyte quality
Cumulus cells
Ooplasm
High quality cocs
Oocyte maturation

IVF (In Vitro Fertilization)

Coincubation of motile sperm and COCs
Denudation of presumed zygotes next day by vortexing

IVC (In Vitro Culture)

45-50% blastocysts at day 8 in group culture
30-35% blastocyst at day 8 in individual culture

Quality control of embryos

Invasive : staining the cell number
Non invasive : time lapse video for cleavage

Surgical embryo flushing

Surgery in sow
80-90% of embryos can be flushed

Take home messages

Caveat frozen semen : less piglets
No for sexed semen : only feasible with “deep AI”, low numbers