Page 1 of 12 Pharmacognosy Department-College of Pharmacy ~“TANTA UNIVERSITY ac : E> FACULTY OF PHARMACY. . DEPARTMENT OF PHARMACOGNOSY aod TERM: | SE HOURS = 205 marks, 95 minutes), ‘omplete (45 marks, 25 minutes). Answers outside specified places will be neglected. Use blue pen, Answers using pencils will not be accepted. Answer sheet albtela iS 42 43 44 tt 45 46 47 48 49 50 (ele) sJalalalelel=[yPage 2 of 12 Pharmacognosy Department-College of Pharmacy Part 205 marks, 95 minutes: You are provided with 82 MCQs. Select only one answer and mark your choice in answer sheet in page 1 1- From Q. No. 1-14.,.... Carbohydrates (35 marks, 15 minutes) 2- From Q. No. Glycosides (67.5 marks, 30 minutes) 3- From Q. No. Bitter principles, tannins and terpenoids (67.5 marks, 35 minutes) 4- From Q. No. 69-82... Vitamins, hormones, tissue culture and microbial transformation (35 marks, 15 minutes) 1- Carbohydrates... . (35 marks, 15 minutes) 1- Which of the following is composed of galactose, glucose, and fructose? a- Cellobiose b- Stachyose ¢- Trehalose d- Lactose e- Raffinose 2 Hydrolysis of and D-xylose. a- Gum tragacanth b- Gum Arabic c- Alginic acid d+ Quinovose e- Laminarin yields D-galacturonic acid, D-galactose, L-fucose, L-arabinose . is used for the formulation of eye drops indicated for the symptomatic treatment of lachrymal insufficiency cleroglucan b- Dextrin| c- Dextran entinan e- Agarose 4. Match each sentence (Q 4-6) with only one suitable structure in List 1, --(4)....is one of the sugars specific to cardiac glycosides, ~s-+(8)...is believed to contribute to the pain relief of cartilage and structure rebuilding of cartilage in the joint. +--1(6)....i8 produced by reduction of mannose. seed Fo CHOH | G08 CHa rea | HO-C-H H=C-OH HO-C-H | H-C-OH H-C-OH H-C-OH | w-¢-on H-¢—on HoH HC-0H | cy CH,OH G40H be a e is a heavily sulfated polymer of D-galactose (a1->3, and B14). a- Lentinan b-Carrageenan ——c- Laminarin ¢- Laminarin e- Dextran is used to measure intestinal absorption (by measuring urinary excretion). a- Fructose b- Xylose c- Arabinose d- Mannitol e- GlucosePage 3 of 12 a- Lactulose d- Sucralfate Pharmacognosy Department-College of Pharmacy b- Trehalose e- Cellobiose c- Sucrose is used to treat and prevent stomach and duodenal ulcers, Match each sentence (Q 10-12) with only one suitable structure in Li -...(10).... is an antitumor polymer isolated from the fungus, Lentinus edodes. ~..(11)...is prepared from seaweeds, from Laminaria spp --(12)...is important as a support in affinity chromatography. List2 5. 5 ,= —— % Ie atest ters G | 3 b- © cise AM * "i O- | HO\H 7 ke k i i oy : -,can modify solubility and dissolution rate of pharmaceutical preparations. a- Dextrin b- Stachyose ¢- Dextran d- Cyclodextrins e- Inulin 14- Which of the folloy is incorporated into many antacid preparations to protect against gastro-oesophageal reflex? a- Mucilage d- Gum tragacanth 2- Glycosides... b- Gum Arabic e- Chitosan c- Alginic acid (67.5 marks, 30 minutes) 15- Compound No. (1): a- Has weak cardiotonic activity b- Give —ve Kedde test c+ Relatively absorbed from GIT i d- Biosymthesized from squalene e+ Proscillaridin A LOteandros 16- Compound No. (2): a- Gives +ve Keller-Kiliani reaction: b- Secondary glycoside c- Occurs in D. purpurea d- Weakly absorbed from GIT €- Soluble in chloroform a 0 fo Bras @ sorPage 4 of 12 Pharmacognosy Department-College of Pharmacy 17- Hypokalemia increases the toxicity of cardiac glycosides because: : a- Enhances digitalis-induced increases in intracellular calcium b- Increases the negative chronotropic effect ¢- Sensitizes the heart to digitalis-induced toxicity d- Has -ve effect on cardiac output, and venous return, e+ Increased digoxin binding to the Na'/K*-ATPase cf 18- Compound No. (3): a+ Has weak cardiotonic activity 2 é b- Has moderate cardiotonic activity c- Has strong cardiotonic activity oS d- Has no cardiotonic activity ee e- Secondary glycoside 19- Compound No. (4): a- Occurs in Purple foxglove b- Occurs in Squill c- Gives +ve Keller-Kiliani reaction d- Gives -ve Baljet reaction ¢- Proscillaridin A pone 20- Aquaretic herbs are used to: a- Avoid the increase in toxicity of cardiac glycosides due to the lack of potassium b+ Increase positive inotropic effect of cardiac glycosides c- Replace K sparing diuretics d- Both a and ¢ e- Increase digoxin binding to the Na‘/K”-ATPase ar 21- Compound No. (5): Ho. a- A Flavone b- Belongs to aurones CO I c- Has phytoestrogenic activity © d- Used in ovarian cancer therapy ug e- Both cand d 22- Which of the following compound has the least antioxidant activity? cH " oe oo "O AR a “ iy HOA no KA Ana Oty 1 OCH, OHO i OH 0 ® ay oH ‘ A>? ] ‘oH Ho’ © ‘0-Glu-Rham. bo 23- Compound No. (6): ¢- Isovitexin d- Neuroprotective e- Botha and dPage 5 of 12 Pharmacognosy Department-College of Pharmacy 24- Compound No. (7): a- Pigment of red fruits b- Effective in hyperpigmentation disorders oH c- Not strictly flavonoid d- Has phytoestrogenic activity in vitro, , Ho Ao. ¢- Abundant in Citrus fruit OO. o bn 25- Tamarixetin is: a Quercetin methylated at position O-4 b- P-glycoprotein inhibitor c- Both a and b d- Polymeric eatechin e- Inhibit cyclo-oxygenase oH AYO 26- Compound No. (8): a+ Strengthen blood vessels, - Flavanol ‘OH c- Quercetin d- Flavone e- Isoflavone ‘O-Rutinose ¢@) OH O 27- This biosynthetic step represent a part HQ cH Fi of biosynthesis of: a- Flavonoids [SH.COOR =>; sl be ) - Saponins HOCH, COOH. © c- Cardiac glycosides d- Thioglycosides e- Anthraquinones 28- Compound No. (9) is considered: - as tp a An oleanane-type triterpene saponin = one b- An ursanes-type triterpene saponin c- Lanostanes-type triterpene saponin co) d+ Lupanes-type triterpene saponin e- a-amyrin derivatives soa 29- Compound No. (10): ‘a+ Gives blue-green with Liebermann reaction b- Gives red color with Liebermann reaction c- Has antifungal effect d- Has spermicidal activity ¢- Antitussive and expectorant 30- Compound No. (11): a- Water-soluble pentacyclic steroidal saponin b- Has sweetening power ¢ Doesn't use in patient with hypertension. ¢- Anti-inflammatory and antiarthritic ¢- Anticancer 31- European Pharmacopoeial assay defined the hemolytic value as a- Characterization Value ‘be A method for quantitation c- Both a and b d- Universal method applied to all types of saponins e- A method applied instead of colorimetry and spectrophotometryPage 6 of 12 Pharmacognosy Department-College of Pharmacy, 32- This biosynthetic precursor is used for biosynthesis of: Nie a- Sinigrin b- Sinalbin Hos: ‘oH c- Saponins ¢- Thioglycosides e- None of them HO. 0H 0 33- Compound No. (12): a- On hydrolysis yield benzyl isothiocyanate and glucose ‘OH b- Has protective effect against colon cancer oH HO" 7 es ¢- Glucotropaeoline t d- Suppresses NO production N (12) e- Occurs in Black Mustard seeds % 34- No convincing evidence showing that amygdalin induces rapid and distinet tumor regression in cancer patients: a- True b- False 35- Compound No. (13): COOCH; a- Occurs in Salix species b- Hydrolyzed by the enzyme emulsin omar c- Did not cause gastric ulcer d- Release salicylate in intestine slowly a3) e- Both c and d 36- Compound No. (14): a- Gives blue colour with concentrated sulphuric acid b- Inhibits osteoclast differentiation and bone resorption a, a4) c- Occurs in Populus species d- Decreases generation of cyclo-oxygenase and 5-lipoxygenase mnetaboltes e- Astringent glycoside in citrus. 37- Compound No. (15): biosynthesized via acylpolymalonate b- Is biosynthesized via shikimie acid J c- Known as chrysophanol (1s) d- Both a and ¢ e- Both b and c 2 bi 38- Compound No. (16): a- Can be hydrolyzed using HCI " b- Can be hydrolyzed by f-D-glucosidase enzyme c- Mixture of 2 isomers 4- Both b and ¢ e- All of the above 39- In compound No. (17), if R= CH2OH and 10,10°(trans), It a- Sennosides A b- Sennosides B c- Sennosides C d- Sennosides D e- None of them 40- In Compound No. (18), if R= OH, Itis... a- Casaroside A b- Casaroside B c- Casaroside D d- Casaroside C e- None of them Ho- n ‘* is) OHPage 7 of 12 Pharmacognosy Department-College of Pharmacy 3. Bitter principles, tannins and terpenoids ... +.(67.5 marks, 35 minutes) 41- Compound No. (19): a- Is optically active be Its solution in olive oil is vesicant to skin c+ Can be erystalized from CCl, d-+ furfural + H;SO,— brown to black color e- Both b and d 42- Compound No. (20): a- Chemical class is triterpene bb Its source is the stem bark of Picrasma excela family Simarubaceae. ¢- + HpSOs+ Sucrose — red color d- Has anti-tumor effect e- Both a and ¢ Match each sentence in (Q 43-45) with only one suitable structure in List 3. ~ Derivatives of ...(43)... are used as antileishmanial and P-glycoprotein inhibitory activity. ~ ..:(44)...+ HySOy — no color + FeCls + violet color. ~ «1.(45)... + H2SO, — golden yellow — reddish brown, a b- e a e 46- The chemical class of humulone is ..... a- Inner anhydride b- Phloroglucinol derivatives ¢- Chromone d- Coumarin e- Coumarone 47- All of the following compounds give white precipitate with ammonical silver nitrate (Tollin's reagent) EXCEPT. a-Ammoidin — b- Picrotoxin ¢-Humulone d= imperatorin. e+ Asarone 48- To identify two types of tannins occur in the extract, we can use a FeCls b-Gelatin c-Goldbeater’s skin d-Phenazone—_e- Br; water. 49- Goldbeater’s skin test a- With gallic acid —+ a brown or black color is developed on the skin. b- A membrane prepared from intestine of Ox soak in 2% HCI. ¢- Used as qualitative and quantitative test for tannin, d- Both b and ¢ e- All of themPage 8 of 12 Pharmacognosy Department-College of Pharmacy on An 50- Compound No. (21) is all of the following EXCEPT. | a- Is the precursor of condensed tannins ww b+ NH,OH — Green color ¢- Was first isolated from the tannin of Acacia catechu. ai 4 Results from the combination of acetate ond cinnamic acid pathways fe Does not precipitate gelatin except after heating with mineral acid for a short time 51- Determination of tannins can be done using. a- Copper acetate b- Gelatin + Hide powder d- Both a and © e- Both a and b 52- Eucalyptus leaves contain..... ‘a- Leueocyanidin b- Gallotannin c- Ellagitannins d+ Catechin e- Both cand d 53- Condensed tannins differ fundamentally from gallotannins and ellagitannins by being structurally related to the flavonoids. a- True b- False 54- On dry distillation, hydrolysable tannins yield—. ° OH . wpe a - ou SS Wo 1 Ho on AA 4 ead HO” t ‘OH OOK OH = Ko! CH 55- The iridoid glycoside is obtained from the fruits of Gardenia jasminoids (Rubiaceae) can be used as . a- Hepatoprotective b- An antitumor c- Anti-inflammatory and analgesic d- Antimicrobial e- None of them ‘56- Valepotriates are all of the following EXCEPT a+ They have an epoxy ring and a triester functionality. b- Present in the roots of Valeriana officinalis c- Conjugated dienes (didrovaltrate) and non-conjugated dihydroderivatives (valtrate), d- Sedative and tranquilizer agents. e- Non-glycosidic iridoids 57- Taxol comprises a diterpene core, three side chain bearing aromatic rings and ‘Two acetoxy moities. It is used in treatment of ovary and breast cancer a True b- False 58- Compound No. (22): a-Is found in excess in Gossypium barbadense be Is considered as sesquiterpene phytoalexin c- Pharmacological inactive and has toxic effect. &- Both a and b ¢- Both b and cPage 9 of 12 Pharmacognosy Department-College of Pharmacy 59- An example of biologically active norditerpenoids. a- Compound 23 b- Compound 24 c- Compound 25. d- Compound 27 e- Compound 29 60- Labdane diterpene that increases cAMP. ‘a- Compound 23. b- Compound 24 c- Compound 25. d- Compound 27. e- Compound 29 61- ...uis a diterpenoid used as plant hormone. a- Compound 23 b- Compound 24 ¢- Compound 25 d- Compound 27 e- Compound 29 62- Sweetening agent. ‘a-Compound 23. b- Compound 24 c- Compound 25 d- Compound 27 e- Compound 29 63. . is obtained from Devil's Claw and has been widely used in Europe to help, support healthy joint function. a- Compound 26 b- Compound 28. c- Compound 30 d- Compound 31 e- Compound 32 64- A sesquiterpene nutraceutical is used for glycemic control. ‘a- Compound 26 b- Compound 28 ¢- Compound 30 d- Compound 31 ¢- Compound 32 65- ..... acts as blood schizonticide in treatment of malaria and is effective against chloroquin resistant malaria. a-Compound 26 b- Compound 28 c- Compound 30 d- Compound 31 e- Compound 32 66- ...... Was found to be the active agent from the leaves of feverfew, Tanacetum parthenium (Family Asteraceae). a- Compound 26 b- Compound 28. c- Compound 30 d- Compound 31 e- Compound 32 67- An example of diterpene that acts as platlet activator factor antagonist and is used in treatment of peripheral vascular ischemia. a-Compound 23 b- Compound 24 ¢- Compound 25. d- Compound 27_¢- Compound 29 68- The aglycone of .......has antibacterial activity against Staphylococcus aureus beside an antitumor aetivity. a- Compound 26 b- Compound 28 c- Compound 30 d- Compound 31 e- Compound 32 SGlucose|Page 10 of 12 Pharmacognosy Department-College of Pharmacy, 4- Vitamins, hormones, tissue culture and microbial transformation (G5 marks, 15 minutes) is (are) a source of vitamins A & D. a- Rose fruits ’b- Green vegetables c- Citrus fruits d- Dried yeast e- Cod liver oil 70+ Cx; pregnane skeleton, 17B-CO.CHOH side chain, 11B-hydroxyl and 17 hydroxyl are characteristic features of .. a- Aldosterone d- Estrogen 71- Exophthalmos results from ..... a- Thyroid hypofunction c- Adrenal cortex hypofunction e- Adrenal medulla hyperfunction Match each sentence (Q 72-74) with only one suitable structure in List 4 69- b-Testosterone c- Cortisol ¢- Progesterone b- Thyroid hyperfunetion - Adrenal cortex hyperfunction -...(72)....is involved in the blood-clotting system. =1..(73)..._ its Deficiency results in scurvy ~.._,(74)...is found in vegetable fats an¢ oils (germ oil of cereals) and acts as antioxidant. Cy 75- Sterilization of plant tissues for tissue culture is performed usin; c- Disinfectant a- Autoclave b- UV rays d- Dry heat e+ Liquid Ny 76- The undifferentiated plant cells grown in the tissue culture is called... a- Plantlet b- Callus c- Explant d- Rootlet ¢- Protopalast 77-Mamoli and Vercellone discovered that yeast can reduce A* - androstenedione to.. a- Aldosterone b- Estrogen c- Cortisol d- Testosterone ¢- Progesterone 78- During the lag phase of the bacterial growth, the microorganisms... a- Adapt themselves to the new environment _b- Undergo rapid growth ¢- suffer from lack of mutrients d+ begin to die ce Manage to survive for relatively long periods 79- Macroelements that must be included in the culture media are..... a- Nitrogen, phosphorus & iron b- Nitrogen, phosphorus & caleium ¢- Phosphorus, calcium & copper d- Calcium, potassium & boron ¢- Iron, manganese & molybdenumPage 11 of 12 Pharmacognosy Department-College of Pharmacy Match each sentence (Q 80-82) with only one suitable structure in List 5 ~...(80)....is produced by using cortisol as a substrate. 81)... its Deficiency results in Parkinson's disease -....(82)....is an example of phytoestrogens. ist 5 On ° i I Ho 0 So b OH HO. HO. NH. 2 Ho 2 el NH a e Part Il Chromatography... Complete the following sentences and write vour answers in the answer table in page 12 ~The retention volume is increased by... (I) ... the affinity to the mobile phase. ~The adsorption of the stationary phase is ... (2)... at higher temperature- -Column chromatography is used for determination of amino end group of proteins, after reacting the proteins first with ...... (3) -. - When the paper sheet, after loading the sample is placed in the proper container with the mobile and stationary phase for several hours before development this called (4)...which cause ... (5)...the rate of flow . (6) .... consists of filter paper dises of the same diameter, stacked inside column. - One of the advantages of paper over TLC is ... (7).-+ ~ Reis used instead of Ry value in these techniques ... (8)... and ... (9)--. ~ The degree of separation of solute bands in chromatogram is called... (10). - In reversed phase chromatography we can use adsorbents as... (11) - Revalues of the samples are decrsased than the normal due to... (12) - NHAOH vapors are used for detection of... (13)... and gives ... (14)...color - Periodate spray at first, then spray with benzidine is used for the detection of ...(15)...and gives > ...(16)... - The advantages of gradient elution are ... (17)... and ...(18)... - Instrument used for quantitative determination of radioactivity in chromatographic path is called ...(19)... - The factors affecting the development on TLC are ... (20)... and ... (21)... ~ The technique used for the separation of closely related compounds in mixture is ...(22)... - Kieselguhr is used for ......(23). -In paper chromatography, sample can be purified by......(24)......0 -We can't use 10% HySO, sprayer with ...(25)... adsorbent because ...(26) ... = Liquid chromatogram represents the result of ...(27) - Polyamide adsorbent is useful in chromatographic separation of ... (28) ...,While charcoal is useful for separation of ... (29) .... - Horizontal Development gives ...(30) ... Revalues.Page 12 of 12 Pharmacognosy Department-College of Pharmacy, Answer Table No. | Correct word (s) No. Correet word (s) a 16 | L 4 i 2 | 17 3 18 4 19 4 5 20 | | i 6 ne 7 22 8 23 9 | 24 | | | | | 10 25 | m1 26 | | [12 27 | 113 28 14 29 | | | [15 | 30 | l oe | Good Luck