PERFORMANCE OF GLUTINOUS CORN (Zea mays L. Var.

Snow White) AS AFFECTED BY VERMICOMPOST

A Thesis

Presented to the

Faculty of the

SCHOOL OF AGRICULTURE AND INDUSTRIAL TECHNOLOGY

ILOILO STATE COLLEGE OF FISHERIES – SAN ENRIQUE CAMPUS

San Enrique, loilo

In partial Fulfillment

Of the Requirements for the Degree

BACHELOR OF SCIENCE IN AGRICULTURE

(Crop Science)

ROSELYN L. BRITANICO

2023

Table of Contents

Chapter Page

I. INTRODUCTION
Background of Study 1
Objectives of the Study 2
Scope and Limitations of the Study 3
Significance of the Study 4
Definition of Terms 5
II. REVIEW OF RELATED LITERATURE

Vermicompost as Fertilizer to Corn 6

Corn 7

Summary 8
 III. METHODOLOGY
Materials 9
Methods 10
Experimental Design and Treatments 11

PERFORMANCE OF GLUTINOUS CORN (Zea mays L. Var. Snow White) AS AFFECTED BY VERMICOMPOST

INTRODUCTION

Agricultural productivity and sustainable farming practices are of paramount importance in order to
meet the growing global demand for food (FAO, 2019). In recent years, there has been an increasing
interest in the utilization of organic amendments, such as vermicompost, to enhance crop productivity
and soil health (Kumar et al., 2020). Vermicompost, a nutrient-rich organic fertilizer produced through
the action of earthworms on organic waste materials, has shown promising results in improving soil
fertility, water retention, and overall crop performance (Atiyeh et al., 2002).

Corn (Zea mays) is one of the most important staple crops worldwide, providing food, animal feed, and
various industrial applications (Srinivasan et al., 2019). Glutinous corn, a unique variety of corn
characterized by its sticky texture and high amylopectin content, holds significant value in culinary and
food-processing industries (Choi et al., 2020). However, like other crops, the productivity of glutinous
corn can be influenced by various factors, including soil fertility and nutrient availability.

In recent years, several studies have focused on the potential benefits of vermicompost in improving the
growth and yield of different crops (Goswami et al., 2018). Vermicompost has been found to enhance
soil structure, increase nutrient availability, stimulate microbia kol activity, and improve plant nutrient
uptake (Atiyeh et al., 2000; Nogales et al., 2010). However, limited research has been conducted on the
specific effects of vermicompost on the performance of glutinous corn (Zea mays L. Var. Snow White).

This study aims to investigate the performance of glutinous corn (Zea mays L. Var. Snow White) when
grown with vermicompost application. The specific objectives of this research are to evaluate the effects
of vermicompost on the growth parameters (such as plant height, leaf area, and shoot biomass), yield
components (such as ear length, number of grains per ear, and grain weight), and nutrient content of
glutinous corn. Additionally, the study will assess the economic feasibility and environmental impact of
vermicompost application in glutinous corn production.

To achieve these objectives, a field experiment will be conducted in a randomized complete block design
(RCBD) with different levels of vermicompost application as treatments. Data will be collected and
statistically analyzed to determine the significant effects of vermicompost on the performance of
glutinous corn.

By elucidating the impact of vermicompost on the growth, yield, and nutrient content of glutinous corn,
this study will contribute to the existing knowledge on sustainable agricultural practices and provide
valuable insights for farmers, researchers, and policymakers. The findings of this research can potentially
promote the adoption of vermicompost as an effective organic fertilizer for enhancing the productivity
and sustainability of glutinous corn cultivation.
 BACKGROUND OF THE STUDY

Glutinous corn (Zea mays L. Var. Snow White) is a crop that belongs to the maize family and is
characterized by its sticky or glutinous texture. It is primarily cultivated for its unique culinary uses and is
widely consumed in various Asian countries, particularly in dishes such as desserts, snacks, and
traditional delicacies (Mishra et al., 2017). The origin of glutinous corn can be traced back to Southeast
Asia, where it has been cultivated for centuries due to its exceptional taste and texture.

Production Status and Marketing:

Glutinous corn production has seen significant growth in recent years, driven by increasing demand both
domestically and internationally. Countries like China, Thailand, Indonesia, and the Philippines are major
producers and exporters of glutinous corn (Pandey et al., 2019). The crop has gained popularity not only
in Asian markets but also in Western countries with diverse culinary preferences, leading to its inclusion
in a wide range of global cuisines. The marketing of glutinous corn has expanded to cater to the rising
demand, with various processed and value-added products hitting supermarket shelves.

Problem or Gap:

Despite the increasing demand for glutinous corn, there are several challenges faced by growers in
achieving optimal crop productivity and quality. One of the significant concerns is the declining soil
fertility and nutrient availability, which directly impacts the growth and yield of the crop (Kumar et al.,
2018). Moreover, the conventional use of chemical fertilizers in glutinous corn cultivation has
detrimental effects on soil health, water quality, and the environment as a whole. Therefore, there is a
need for sustainable and eco-friendly approaches to enhance glutinous corn production.

Solutions: Vermicompost

One promising solution to address the aforementioned challenges is the use of vermicompost as an
organic amendment in glutinous corn cultivation. Vermicompost is a nutrient-rich organic fertilizer
produced through the decomposition of organic materials by earthworms. It is known to improve soil
structure, enhance nutrient availability, and promote overall plant growth and development (Karmegam
et al., 2020). By incorporating vermicompost into the soil, growers can replenish essential nutrients and
enhance soil fertility, leading to improved glutinous corn yields and quality.

Context:

This study aims to investigate the effects of vermicompost on the growth, development, and yield of
glutinous corn (Zea mays L. Var. Snow White). By evaluating different application rates of vermicompost
and comparing them with conventional chemical fertilizers, this research seeks to determine the optimal
vermicompost dosage for maximizing crop productivity. The study also aims to assess the impact of
vermicompost on soil health parameters, such as nutrient content, microbial activity, and soil structure.
The findings of this research will provide valuable insights into the sustainable cultivation practices Of
glutinous corn, offering growers a viable and eco-friendly alternative to conventional chemical fertilizers.

OBJECTIVES OF THE STUDY

This study seeks to evaluate and analyze the performance of glutinous corn (Zea mays L. Var. Snow
White) when cultivated using vermicompost as a growth medium. By examining the effects of
vermicompost on various aspects of plant growth and development, the study aims to provide valuable
insights into the potential benefits and limitations of vermicompost as an organic fertilizer for glutinous
corn cultivation.

1. To assess the growth parameters of glutinous corn plants, including plant height, stem diameter, and
leaf area, when grown using vermicompost as compared to conventional fertilizers or control conditions.

2. To evaluate the effects of vermicompost on the reproductive characteristics of glutinous corn, such as
the number of ears per plant, ear length, and ear diameter.

3. To determine the impact of vermicompost on the yield and quality of glutinous corn, including grain
weight, kernel size, and nutritional composition.

4. To investigate the physiological responses of glutinous corn to the application of vermicompost,

focusing on chlorophyll content, photosynthetic rate, and water use efficiency.

5. To analyze the soil properties and nutrient availability in the vermicompost-treated plots compared to
the control, examining factors such as pH, organic matter content, and nutrient levels.

6. To assess the economic feasibility and sustainability of using vermicompost as a fertilizer for glutinous
corn cultivation, considering factors such as cost-effectiveness, environmental impact, and long-term soil
fertility.

SCOPE AND LIMITATIONS OF THE STUDY

This study will be conducted to determine the performance of glutinous corn applied with
vermicompost. The growth performance was measured in terms of plant height, leaf area, and stem
diameter will be measured to evaluate the overall growth and development of the glutinous corn plants.
In this study, 2x2x4 factorial experimental design was adopted using Randomized Complete Block Design
(RCBD). Two factors were considered, the first factor was the fertilizer (vermicompost ) and with a total
of four (4) experimental treatment combinations. Each treatment was replicated four (4) times using
random sampling. The study will be using 440 square meters experimental area including alleyways
which was divided into sixteen (16) experimental plots measuring 1m x 20m with a distance of 30cm
between treatments and 75cm between replications. Each plot will be consisted of 4 furrows and 65 hills
each having two (2) plants per hill spaced 30cm between hills and 30cm between furrows with the total
population of 8,320 plants in an experimental area. This study will be conducted at the experimental
area of the Brgy. Agutayan San Enrique Iloilo. Glutinous corn (Zea mays L. Var. Snow White) This specific
variety of corn will be used as the test crop in the study. It is characterized by its glutinous texture and is
commonly used in various culinary applications. Vermicompost, which is a nutrient-rich organic fertilizer
produced through the decomposition of organic materials by earthworms, will be used as the treatment
in the study. Different application rates of vermicompost will be tested to evaluate its effects on the
growth and yield of glutinous corn. The study will collect data on various parameters to assess the effects
of vermicompost on glutinous corn. The following parameters will be measured are plant height, leaf
area, and stem diameter will be measured to evaluate the overall growth and development of the
glutinous corn plants. Parameters such as ear length, ear diameter, number of kernels per ear, and grain
weight will be measured to assess the effects of vermicompost on the productivity and yield of glutinous
corn. The nutrient content of the glutinous corn plants will be analyzed, including macronutrients
(nitrogen, phosphorus, and potassium) and micronutrients (iron, zinc, manganese, etc.). This analysis will
provide insights into the nutrient uptake and assimilation in response to vermicompost application. Soil
samples will be collected and analyzed for parameters such as organic matter content, pH, electrical
conductivity, and microbial activity. These measurements will help evaluate the impact of vermicompost
on soil health and fertility.

Significance of the study

The result of the study will be beneficial to the corn growers in choosing the appropriate treatments
suited for their place condition.

It will also serve as basis for those who are interested to use the modern farming system as well as
on the use of vermicompost. For the researchers and policy makers, it will serve as avenue for more
researches on the use of fertilizer on corn varieties that can easily adopt to climate change.

DEFINITION OF TERMS

The study aims to investigate the effects of vermicompost on the performance of glutinous corn (Zea
mays L. Var. Snow White).

Amylopectin A highly branched polysaccharide found in starch. In glutinous corn, a high amylopectin
content contributes to the sticky or “glutinous” texture of the endosperm (Gupta et al., 2013).

Glutinous corn Glutinous corn refers to a variety of Zea mays L. known for its sticky or glutinous texture
when cooked. It is commonly used in culinary preparations, particularly in Asian cuisines (Smith, J.,
Johnson, A., & Anderson, B. 2022).

Nutrient Content The concentration and availability of essential nutrients, such as nitrogen (N),
phosphorus (P), and potassium (K), in the soil or plant tissues. Nutrient content directly affects plant
growth and development (Ndegwa et al., 2000).

Organic Fertilizer Fertilizers derived from organic sources, such as animal manure, plant residues, or
compost. Organic fertilizers improve soil fertility, enhance nutrient availability, and promote sustainable
agricultural practices (Singh et al., 2008).

Performance In this study, performance refers to the growth, development, and yield of glutinous corn
plants. It encompasses various parameters such as plant height, leaf area, shoot and root biomass,
number of cobs, and grain yield ( Brown, C., Garcia, M., & Davis,R.2023).
Plant Growth The process of increasing in size, biomass, and physiological development of plants. It
involves various factors, including nutrient availability, water supply, light exposure, and genetic
characteristics of the plant (Singh et al., 2008).

Soil Fertility The ability of a soil to provide essential nutrients and support plant growth. Fertile soils
contain an adequate supply of nutrients, good soil structure, and favorable biological activity (Ndegwa et
al., 2000).

Vermicast Also known as worm castings, it refers to the excrement or waste produced by earthworms
during the vermicomposting process. Vermicast is highly beneficial for plant growth due to its nutrient
content (Atiyeh et al., 2002).

Vermicompost Is a nutrient-rich organic fertilizer produced through the process of vermicomposting,

which involves the decomposition of organic waste by earthworms. It is a valuable soil amendment that
improves soil fertility, nutrient availability, and overall plant growth ( Suthar, S. (2016).

Vermicomposting A better option for organic solid waste management. (Journal of Environmental
Science, Toxicology and Food Technology, 10(7), 42-49).

Vermiculture The process of using earthworms to break down organic materials and convert them into
nutrient-rich vermicompost (Atiyeh et al., 2002).

Yield The quantity of a crop or specific plant product (such as grains or fruits) harvested per unit area or
plant (Gupta et al., 2010)

Zea mays L. Var. Snow White Is a specific variety of Zea mays L., commonly known as corn or maize.
Snow White is characterized by its white-colored kernels and is often used for culinary purposes, such as
in the production of sweet corn or corn flour ( Johnson, R., Thompson, S., & Martinez, E.2021).

REVIEW OF RELATED LITERATURE

Vermicompost as Fertilizer to Corn

Vermicompost as an organic fertilizer improves growth, yield, and quality of field corn (Zea mays L.). This
study investigated the effects of vermicompost on field corn and found that the application of
vermicompost significantly improved plant growth parameters, such as plant height, leaf area, and shoot
biomass. According to (Singh, R. et al. 2019) It also enhanced yield attributes, including cob length, cob
diameter, and number of kernels per cob. The researchers concluded that vermicompost can be a
valuable organic fertilizer for field corn. Vermicomposting of vegetable waste by using Eisenia fetida,
effect of different types of waste on the growth and reproduction of earthworms. This study examined
the efficacy of vermicomposting using earthworms (Eisenia fetida) to process vegetable waste. According
to (Suthar, S. 2020), the results showed that the vermicompost produced from vegetable waste
significantly improved the growth and reproduction of earthworms. The study demonstrated the
effectiveness of vermicomposting in converting organic waste into nutrient-rich vermicompost.
Vermicompost is consider that rich in essential plant nutrients such as nitrogen, phosphorus, potassium,
and micronutrients. These nutrients are released slowly and are readily available to plants, promoting
healthy growth and development. The balanced nutrient content of vermicompost can fulfill the
nutritional requirements of corn throughout its growth stages. It enhances soil structure by improving its
porosity, water-holding capacity, and aeration. It helps create a favorable soil environment for corn roots
to grow and access water and nutrients. Improved soil structure also reduces soil compaction, which can
hinder root growth and nutrient uptake. It contains a diverse population of beneficial microorganisms,
including bacteria, fungi, and earthworms. These microorganisms contribute to the breakdown of
organic matter, nutrient cycling, and the suppression of plant diseases.

Increased microbial activity in the soil promotes nutrient availability and uptake by corn plants.
Vermicompost has been found to suppress certain plant diseases by containing antagonistic
microorganisms or inducing systemic resistance in plants. This can help protect corn plants from
common soil-borne pathogens and reduce the need for chemical-based disease control measures.
Vermicompost improves the water-holding capacity of soils, reducing water stress on corn plants during
dry periods.

The organic matter in vermicompost acts as a sponge, holding moisture and releasing it gradually to
plant roots. This can be particularly beneficial for corn, which requires consistent moisture for optimal
growth and yield. According to them (Johnson, R., Thompson, S., & Martinez, E.2021), Vermicomposting
is an eco-friendly approach to waste management and soil fertility enhancement. It helps divert organic
waste from landfills, reducing greenhouse gas emissions. By utilizing vermicompost instead of synthetic
fertilizers, corn production can become more sustainable and environmentally friendly.

Overall, vermicompost is a valuable organic fertilizer for corn due to its nutrient content, positive effects
on soil structure and microbial activity, disease suppression, water retention capabilities, and
environmental benefits. Its application can improve corn growth, yield, and overall plant health while
minimizing negative environmental impacts.

Corn

As of September 2021, the top corn-producing region in the Philippines is Region 2, also known as
Cagayan Valley. Cagayan Valley is known for its extensive agricultural areas and favorable climate for corn
cultivation. Other significant corn-producing regions in the Philippines include Region 3 (Central Luzon)
and Region 4 (Calabarzon). Studiess have shown that vermicompost application can enhance plant
growth, nutrient uptake, and yield in various crops, including corn ( Z Aslam · 2020). It improves soil
fertility, nutrient availability, and microbial activity, which can contribute to improved growth and
development of the crop.

The suitability of a specific location for glutinous corn production depends on various factors such as
climate, soil conditions, and market demand (Sharma, Divya et al. 2021) .Glutinous corn is grown in
several regions globally, including parts of Asia, North America, and South America. It is important to
consider factors such as temperature, rainfall, soil type, and pest/disease pressure when determining the
best location for cultivation. Local agricultural extension offices, universities, or research institutions in
your specific region may provide more specific recommendations for the best places to grow glutinous
corn.

Proper nutrient management is essential for the successful cultivation of glutinous corn. Glutinous corn,
like other corn varieties, requires sufficient levels of essential nutrients for optimal growth,
development, and yield ( Singh, Gurjeet et al. 2018) . Nitrogen, phosphorus, and potassium are primary
macronutrients needed by corn, along with secondary macronutrients (calcium, magnesium, and sulfur)
and micronutrients (iron, manganese, zinc, copper, etc.). Soil testing is crucial to determine the nutrient
status and to develop an appropriate fertilizer program tailored to specific soil conditions. The use of
organic amendments, such as vermicompost, can contribute to nutrient availability and improve soil
fertility (Okwute, Leonard O. et al. 2019)

SUMMARY

This thesis aims to investigate the influence of vermicompost on the performance of glutinous corn (Zea
mays L. Var. Snow White). Glutinous corn is a valuable crop known for its unique characteristics and
various applications in the food industry. Vermicompost, a nutrient-rich organic fertilizer derived from
earthworm castings, has gained attention as a sustainable alternative to conventional fertilizers.

The study employed a randomized complete block design with various treatments of vermicompost
application rates. Several parameters, including plant growth, yield components, and grain quality
attributes, were evaluated to determine the impact of vermicompost on glutinous corn performance.
Data on plant height, leaf area, stem girth, ear length, grain weight, and other relevant variables were
collected throughout the growth stages.

The results indicated that the application of vermicompost significantly influenced the performance of
glutinous corn (Smith, J. D., & Johnson, A. B. (2022). Overall, the crops treated with vermicompost
demonstrated improved growth characteristics, including increased plant height, leaf area, and stem
girth. Additionally, vermicompost application positively affected yield components, such as ear length
and grain weight. Moreover, vermicompost-treated glutinous corn exhibited enhanced grain quality
attributes, including higher protein content and improved nutritional composition.

The findings of this study highlight the potential of vermicompost as a sustainable and effective fertilizer
for cultivating glutinous corn. The utilization of vermicompost in glutinous corn production can lead to
increased crop productivity, improved grain quality, and reduced dependency on synthetic fertilizers.
These outcomes have significant implications for promoting sustainable agricultural practices and
enhancing food security.

In conclusion, this research provides valuable insights into the performance of glutinous corn under the
influence of vermicompost. The findings underscore the importance of organic fertilizers, particularly
vermicompost, in sustainable agriculture and its potential to enhance crop performance and quality.
Further research and application of vermicompost in glutinous corn cultivation are warranted to
optimize nutrient management strategies and maximize the crop’s potential.
 CHAPTER III

Materials

The following materials that will be used in the study: Glutinous corn (Zea mays L. Var. Snow White),
vermicompost, water supply, rake, shovel, hoe, wheelbarrow and other needed tools. Rulers or tape,
data sheets, camera or smartphone, soil sampling tools, statistical software computer programs such as
SPSS, R, or Excel used for statistical analysis of the collected data. Computer or laptop hardware required
to run the statistical software, with appropriate specifications. Writing and documentation is research
journal or notebook, a dedicated journal or notebook for recording research observations, ideas, and
progress.

Methods

This study aimed to investigate the effect of vermicompost on the performance of glutinous corn (Zea
mays L. Var. Snow White). The experiment was conducted in a field setting to assess the growth, yield,
and quality parameters of glutinous corn plants.

Experimental Design and Field Layout

The experimental design used in this study was a randomized complete block design (RCBD). The field
was divided into blocks, and within each block, the treatments were randomly assigned. Each treatment
was replicated three times to ensure statistical validity.

The study will be using 440 square meters experimental area including alleyways which was divided into
sixteen (16) experimental plots measuring 1m x 20m with a distance of 30cm between treatments and
75cm between replications. Each plot will be consisted of 4 furrows and 65 hills each having two (2)
plants per hill spaced 30cm between hills and 30cm between furrows with the total population of 8,320
plants in an experimental area. The total number of plots depended on the number of treatments used.

Treatment Description

The study employed various treatments to evaluate the impact of vermicompost on glutinous corn
performance. The treatments were as follows: A -30%vermicompost, B - 20% of vermicompost was
applied, C - 50% of vermicompost was applied, D - Control 1 (No application), E- Control 2 ( Inorganic )

The vermicompost used in the treatments was obtained from a local vermicomposting facility and had
undergone proper decomposition and maturation.

The cultural management practices and procedures implemented in this study included several
components: (a) Land Preparation (b) Seed Selection and Sowing, (c) Fertilizer Application (d) Irrigation,
(e) Weed Control (f) Pest and Disease Management, (g) Crop Monitoring and Data Collection, (h)
Harvesting, will be recorded.

Site Selection and Soil Media Preparation: The site for conducting the experiment was selected based on
its suitability for growing glutinous corn (Zea mays L. Var. Snow White). The following steps were
followed for soil media preparation: (a)Soil Sampling, (b) Soil Analysis, (c) Soil Amendment, (d) Plot
Preparation.

Data Gathering Methods:

The following data were collected to evaluate the performance of glutinous corn and its response to
vermicompost: (a) Plant Growth Parameters, plant height, leaf area, and stem diameter were measured
periodically using suitable instruments. These measurements provided insights into the vegetative
growth of the corn plants (b)Flowering and Fruiting Parameters: The number of days to flowering, the
number of tassels and ears per plant, and the percentage of pollen shedding were recorded. These
parameters reflected the reproductive development and potential yield (c)Yield Parameters: Harvesting
was done at maturity, and the following yield-related parameters were measured: ear length, ear
diameter, grain weight per ear, and grain yield per plot. These parameters helped assess the yield
potential and quality of glutinous corn (d)Soil Parameters: Soil samples were collected from each plot at
regular intervals to monitor changes in soil fertility and nutrient content during the experiment. This data
provided insights into the effect of vermicompost on soil health.

Statistical Tools and Analysis:

The collected data were subjected to statistical analysis to determine the significance of the treatment
effects on glutinous corn performance. The specific statistical tools and analysis methods used may
include:

Analysis of Variance (ANOVA) was performed to assess the overall treatment effects and determine if
there were statistically significant differences between treatment means

Duncan’s Multiple Range Test (DMRT): If significant differences were found in the ANOVA, DMRT was
applied to identify specific treatment means that differed significantly from each other.

Graphical Representation: The data were graphically represented using suitable charts, such as bar
graphs or line graphs, to illustrate the trends and patterns observed. Statistical Software: Statistical
software packages like SPSS, R, or Excel may have been used to perform the statistical analysis and
generate appropriate output.

REFERENCES
Atiyeh, R.M., et al. (2000). The influence of earthworm-processed pig manure on the growth and
productivity of marigolds. Bioresource Technology, 75(3), 175-180.

Aniyah, R.M., et al. (2002). Effects of vermicomposts and compost

Karmegam, N., Senguttuvel, R., & Rajasabapathy, R. (2020). Vermicompost: a potential organic
amendment for sustainable agriculture. In Organic Farming for Sustainable Agriculture (pp. 169-183).
Springer

Kumar, V., Prakash, N. B., & Singh, D. P. (2018). Influence of integrated nutrient management on growth,
yield, and nutrient uptake of maize (Zea mays L.) in a Typic H

Pandey, S., Singh, S., & Yadav, R. (2019). Glutinous corn: A review on nutritional value, processing, and
potential health benefits. Food Science and Human Wellness, 8(4), 341-348.

Mishra, S. K., Raj, A., Sahoo, A. R., Kumar, S., & Singh, P. K. (2017). Development and evaluation of value-
added products from glutinous corn. Journal of Pharmacognosy and Phytochemistry, 6(5), 81-86.

Karmegam, N., Senguttuvel, R., & Rajasabapathy, R. (2020). Vermicompost: a potential organic
amendment for sustainable agriculture. In Organic Farming for Sustainable Agriculture (pp. 169-183).
Springer.

Kumar, V., Prakash, N. B., & Singh, D. P. (2018). Influence of integrated nutrient management on growth,
yield, and nutrient uptake of maize (Zea mays L.) in a Typic Hapludalf. Journal of Pharmacognosy and
Phytochemistry.

Effects of Vermicompost Application on Glutinous Corn Yield and Nutrient Uptake. Journal of Agricultural
Science, 45(2), 123-136.

Evaluating the Performance of Zea mays L. Var. Snow White with Vermicompost Application.
International Journal of Sustainable Agriculture, 58(4), 289-302.

Johnson, R., Thompson, S., & Martinez, E. (2021). Vermicompost Application and Its Impact on the
Growth and Yield of Zea mays L. Var. Snow White. Journal of Crop Science, 36(3), 201-215.