POTASSIUM liquirapid Photometric Turbidimetric Test Package Size 0118 100i Complete Test Kit Method Potassium ions in a protein-free alkaline medium react wih sodium tetraphenyboron to produce a finely Gspersed turbid suspension of potassium tetvapheryiboren. The turbidity produced is proportional {o the potassium concentration and read photometicaly. Contents, Reagent Compostion 50 m Precipitant (ite cap) Tthoacete sid TCA) 03 ma som TPe-Ne-Reagent (Sack ep) Sou etepronferon c2men [BOE 0 mi Nao¥ Reagent cap) Soden myo 20m ‘5 mi Standard Potassium (K") 5.0 mmol Feogent Preparation ‘Nbc the content of botle ‘smaller amouns of working reagont mix 011 + 1 (600 "Reagent stability) ‘Allow o stand for 15:30 min. prior fo use. [PREC] and [STD] are ready tor use. [STO] s used undiluted cizectlyin the determination Reagent Stabilty ‘The reagents ae stable up to the given expiry date when stored al 2.25. Tho working reagont is stable for 30 days at 15..25°C and 60 days. 92.8. Specimen 4 ‘Serum and lithium heparin plasma, ‘Assay Wavelength $78 nm, Hg 578 nm Optical path: 11cm Temperature: 20..25°C ‘Measurement: Against reagent blank (Only one reagent blank por soris is roquired Pipetting Scheme Precipitation Pipette into corrfuge tubes: Maco Semmice Specimen 100 nl Ou PREC) 1000 ut 500 ul Mex carefully, centifoge at high speed for 5-10 mn Determination Pipette into cuvettes: TE] Saree Working reagent | 2000 wi | 2000 wt] 1000 ut | 1000 pr 5) zou | -- | tom | — Supernatant 2 200 ut 100 "To produce a homogeneous tufidily, the [STD] or the Gear su pernatant have to be added to the centre of the surface of the ‘working reagent in the cuvette. ix each cwette caretully before proceeding tothe next sample. Alow to stand atleast for 5 min, Measure absorbance of the standard (Af) and the sample (avenge) against working reagent blank between "5 and 30 rirutos, Calculation of the Potassium Concentration c=5x {rmmel or (meg ‘ANGE Linearity ‘The reactions linear upto potassium lvals of 10 mmol. Samples with higher concertratons have fo be aivted 1 +1 with physio: Iogical sti (0.9%). Multiply the recut by 2. Performance Characteristics Typical performance data can be found in the Veriicalion Report, ‘accessible via ‘wor human deldata/gb/w/ey kp or ‘ww human-de.comidata/ghvi/ey-k pat ‘Normal Values ‘Sorum: 36 -5.5 mmo Plasma: 40-48 mmoll Quality Control ‘Al contiol sera with potassium values determined by this method ‘an be used. We recommend to use our animal serum based MUMATROL qual- ly contro sera or our human serum based SERODOS, Notes 41. Use non-hemoltic serum or heparin plasma as specimen. 2. AS red blood cells contain about 25 times the amount of potas sium, they have to be separated from the serum within one hou ‘ater blood collection. Otherwise. falsely elevated potas- sium concentrations wil be found 3. Traces of detergents produce turbidity which leads to falsely elevated potassium concentrations. They therefore have to B® avoided, 4. Contaminated glassware is the greatest source of ror Glassware should therefore be thoroughly nsed witn deionised water. Disposable plastic ware may contain softeners. which react with the reagant and is therefore not recommended 8. (PREC) %; R: 96/98; $: 12-24725-26-45, [RAGA] C; PR: 95; S: 1/2-26-97/90-45 (51D) X; R: 36/98; S: 1/2-24/25-26-45, Reterences. 1. Hilann, 6., Beyer, G., 2. Klin. Chem, Klin. Biochem. 8, 93 (1967), 2 Tietz, N.W., Fundamentals of Clinical Chemistry, Saunders, Philadelphia, 4” Edit, 884 (2006) Soman ce oes eae