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1a [S] mM V (mmol mL-1 min-1)

0.1 3.33 V (mmol mL-1 min-1)


0.2 5 10
0.5 7.14 9
8
0.8 8
7
1 8.33 6
2 9.09 5
4
3
2
1
0
0 0.5 1 1.5 2 2.5

1b 1/[S] mM 1/V (mmol mL-1 min-1)


10 0.3003003003003 Chart Title
5 0.2 0.35
2 0.140056022408964
0.3
1.25 0.125 f(x) = 0.0200269661771655 x + 0.0999804601696732
0.25 R² = 0.999999312609388
1 0.120048019207683
0.5 0.11001100110011 0.2

0.15

0.1
Vmax = 1/0.1 Km = 0.02 x Vmax
Vmax = 10 Km = 0.2 0.05

0
0 2 4 6 8 10 1
n-1)

2 2.5

99804601696732

8 10 12
V (mmol mL-1 min-1)
[S] mM
Sin inhibidor Con inhibidor 1/V
0.2 5 3 0.35
0.4 7.5 5 0.3 f(x) = 0.0533004946652059 x + 0.066825224053013
0.8 10 7.5 R² = 0.999996212251405
0.25
1 10.7 8.3 0.2
2 12.5 10.7 f(x) = 0.0266420586926359 x + 0.066763178791765
0.15 R² = 0.999997558282031
4 13.6 12.5 0.1
0.05
1/V (mmol mL-1 min-1) 0
1/[S] mM 0 1 2 3 4
Sin inhibidor Con inhibidor
5 0.2 0.333333333333333
2.5 0.133333333333 0.2
1.25 0.1 0.133333333333333 Vmax
1 0.093457943925 0.120481927710843 Km
0.5 0.08 0.093457943925234
0.25 0.073529411765 0.08
1/V

= 0.0533004946652059 x + 0.0668252240530137
0.999996212251405

= 0.0266420586926359 x + 0.0667631787917659
0.999997558282031

1 2 3 4 5 6

Con inhibición Sin inhibición


14.97005988024 14.9700598802
0.7979041916168 0.39820359281

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