AVE772 User Manul - 1

AVE-772 User's Manual
AVE-772 Integrated Urinalysis Analyzer
User’s Manual
AVE SCIENCE & TECHNOLOGY CO., LTD.
Building B6, Luguyuyuan, No.27 Wenxuan Road, Changsha Hi-tech Industrial Development Zone, Changsha,
Hunan 410205, P.R.China
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The information in this manual was correct at the time of printing.

However, AVE SCIENCE & TECHNOLOGY continues to improve its products and reserves
the right to change specifications, equipment, and maintenance procedures at any time
without notice.
Companies, names and data used in examples are fictitious unless otherwise noted. No
part of this document may be reproduced or transmitted in any form or by any means,
electronic, mechanical or otherwise, for any purpose, without the express written permission
of AVE. AVE may have patents or pending patent applications, trademarks, copyrights or
other intellectual or industrial property rights covering this document or subject matter in this
document. The furnishing of this document does not give a license to these property rights
except as expressly provided in any written license agreement from AVE.
If this instrument is used in a manner differently than specified in this manual, the protection
provided by the equipment may be impaired.
User Manual for software version 2.2.8.2015
Modification history
Version Date Modification
1.0 2016.12.01 First edition
1.1 2017.7.01 Updated for hardware modification
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1 INTRODUCTION ............................................................................................................................5
1.1 Intended Use ........................................................................................................................5
1.2 How to use the User Manual................................................................................................5
1.3 Precautions and Warnings Symbols – Definitions ...............................................................5
1.3.1 Cautions .....................................................................................................................5
1.3.2 Warnings ....................................................................................................................5
1.3.3 Biological Warnings ...................................................................................................6
1.4 Symbols................................................................................................................................6
1.5 Contact Information ..............................................................................................................7
1.6 Limitation of Liability .............................................................................................................7
1.7 Use of Third Party Computer Products ................................................................................7
2 SYSTEM DESCRIPTION ...............................................................................................................9
2.1 Operation Theory .................................................................................................................9
2.2 Performance and Technical Index .......................................................................................9
2.3 System Components ..........................................................................................................10
2.3.1 System Appearance ................................................................................................10
2.3.2 AVE-772’s structure .................................................................................................12
2.4 Software Functions ............................................................................................................12
2.4.1 Main Programme Screen .........................................................................................13
2.4.1.1 Daily Operation Main Menu ...........................................................................14
2.4.1.2 Management Main Menu...............................................................................14
2.4.1.3 Quality Control Main Menu ............................................................................15
2.4.1.4 Data Main Menu ............................................................................................15
2.4.1.5 Help Main Menu ............................................................................................16
2.4.2 Microscopy and Verification Screen ........................................................................16
2.4.2.1 Urine Real-time Monitoring Screen ...............................................................17
2.4.2.2 Urine Microscope and Verification Screen ....................................................19
3 INSTALLATION ............................................................................................................................21
3.1 Summary ............................................................................................................................21
3.2 Installation Requirements ..................................................................................................21
3.2.1 Space .......................................................................................................................21
3.2.2 Power .......................................................................................................................21
3.2.3 Environmental requirements....................................................................................21
3.2.4 Installation ................................................................................................................22
3.3 System Connection ............................................................................................................22
3.4 Pipeline connection of the analyzer ...................................................................................22
3.5 Power-cable connection.....................................................................................................23
4 SETUP ..........................................................................................................................................24
4.1 User Set .............................................................................................................................24
4.1.1 User login .................................................................................................................24
4.1.2 User Set Operations ................................................................................................25
4.1.3 Password Modification .............................................................................................25
4.2 Microscopy Setting .............................................................................................................26
4.2.1 Item Setting ..............................................................................................................27
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4.2.1.1 Urine Item (Setting) Screen ...........................................................................27

4.2.1.2 Add Urine Items .............................................................................................28
4.2.1.3 Delete Urine Items.........................................................................................28
4.2.1.4 Move Urine Items ..........................................................................................28
4.2.2 Reference Values ....................................................................................................28
4.2.3 Comparison List .......................................................................................................29
4.3 Chemistry Setting ...............................................................................................................30
4.3.1 Chemistry Setting ....................................................................................................30
4.3.1.1 Chemistry Setting screen ..............................................................................31
4.3.1.2 Adding Chemistry Item ..................................................................................31
4.3.1.3 Deleting Chemistry Item ................................................................................31
4.3.1.4 Moving Chemistry Item..................................................................................31
4.3.2 Chemistry Protocol ..................................................................................................32
4.3.2.1 Chemistry Messaging parameter setting ......................................................32
4.3.2.2 Adding Existing Chemistry Protocol ..............................................................32
4.3.2.3 Adding New Chemistry Protocol....................................................................33
4.3.2.4 Adding Comparison Data ..............................................................................33
4.4 Patient Info. Setting ............................................................................................................33
4.4.1 Input Patient Info......................................................................................................33
4.4.2 Print Patient Info. .....................................................................................................35
4.4.3 Show Patient Info.....................................................................................................35
4.5 Mark Color Setting .............................................................................................................36
4.6 Print Format .......................................................................................................................37
4.7 Print Setting........................................................................................................................37
4.8 Tools...................................................................................................................................38
4.8.1 User Settings ...........................................................................................................39
4.8.2 Unusual ....................................................................................................................39
4.8.3 Gallery......................................................................................................................40
4.8.4 Positive List ..............................................................................................................41
4.8.5 MagnGlass On .........................................................................................................41
4.8.6 Test Mode Setting....................................................................................................42
5 SPECIMEN PROCESSING .........................................................................................................43
5.1 Power On ...........................................................................................................................43
5.2 Login...................................................................................................................................43
5.3 Daily Quality Control (Daily QC) ........................................................................................44
5.4 Specimen Preparation .......................................................................................................44
5.4.1 Temperature ............................................................................................................45
5.4.2 Specimen Volume....................................................................................................45
5.4.3 Sample Tube............................................................................................................45
5.4.4 Preparing Sample Racks .........................................................................................45
5.5 Urine test strip preparation.................................................................................................46
5.6 Running Samples ...............................................................................................................47
5.7 Patient Data Entry ..............................................................................................................49
5.8 Dealing with Special Microscopy .......................................................................................49
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5.8.1 STAT Test ................................................................................................................49

5.8.2 Recheck ...................................................................................................................50
5.9 Verification operations .......................................................................................................51
5.9.1 Basic operations ...............................................................................................51
5.9.2 Advanced operations ........................................................................................52
5.9.3 Batch operations ......................................................................................................53
5.10 Print Report ......................................................................................................................55
5.10.1 Single Print.............................................................................................................55
5.10.2 Batch Print .............................................................................................................56
5.11 Power Off .........................................................................................................................56
6 QUALITY CONTROL ...................................................................................................................58
6.1 Quality Control ...................................................................................................................58
6.1.1 Control Material .......................................................................................................58
6.1.2 Control Frequency ...................................................................................................58
6.1.3 Handling QC Material ..............................................................................................58
6.1.4 QC Parameters Input ...............................................................................................58
6.1.5 Running QC .............................................................................................................60
6.2 Remedies on Out-of-control ...............................................................................................61
6.3 Reviewing QC Results .......................................................................................................61
6.3.1 Accuracy Review .....................................................................................................61
6.3.2 Sensitivity Review ....................................................................................................62
6.3.3 Chemistry Review ....................................................................................................63
7 ADJUSTMENT .............................................................................................................................65
7.1 Summary ............................................................................................................................65
7.2 Microscope and Image Adjustment ...................................................................................65
7.2.1 Preparations for microscope focus adjustment .......................................................66
7.2.2 Adjust The Square Mark Position Of Microscope ...................................................66
7.2.3 Focus Adjustment ....................................................................................................68
7.2.4 Image Adjustment ....................................................................................................73
7.3 Pump Calibration................................................................................................................74
7.3.1 Operations ...............................................................................................................74
7.3.2 Caution.....................................................................................................................74
7.4 Blank Calibration ................................................................................................................75
8 RESULTS .....................................................................................................................................76
8.1 Urine Data ..........................................................................................................................76
8.1.1 QC Inquiry ................................................................................................................76
8.1.2 Urine Inquiry.............................................................................................................76
8.1.3 Statistics...................................................................................................................77
8.1.4 History Data Verification ..........................................................................................78
8.2 Tube Records.....................................................................................................................79
8.3 Positive List ........................................................................................................................80
9 MAINTENANCE AND SERVICE..................................................................................................82
9.1 Maintenance.......................................................................................................................82
9.1.1 Precautions ..............................................................................................................82
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9.1.2 Daily Maintenance ...................................................................................................82

9.1.3 Periodic Maintenance ..............................................................................................82
9.1.4 Consumable Replenishment ...................................................................................83
9.2 Daily Maintenance..............................................................................................................83
9.2.1 Cleaning Instrument Surfaces .................................................................................83
9.2.2 QC ............................................................................................................................83
9.2.3 Cleaning Counting Chamber Surfaces ....................................................................83
9.2.4 Cleaning Waste Tank ..............................................................................................83
9.3 Weekly Maintenance ..........................................................................................................83
9.3.1 Cleaning the Sampler ..............................................................................................83
9.3.2 Checking microscope focus (Focus Adjustment) ....................................................84
9.3.3 Cleaning the Strip Conveyor....................................................................................84
9.3.4 Cleaning the Pipette Slot .........................................................................................84
9.4 Monthly Maintenance .........................................................................................................84
9.4.1 Checking pinch valves and moving pinch location in case of adhesion .................84
9.4.2 Cleaning the Waste Pool .........................................................................................85
9.4.3 Pump Calibration .....................................................................................................85
9.4.4 Cleaning Sampling needle.......................................................................................85
9.4.5 Cleaning Photoelectric Sensors on auto-sampler ...................................................85
9.5 Semi-Annual Maintenance .................................................................................................86
9.5.1 Check Microscopic Image .......................................................................................86
9.5.2 Blank Calibration......................................................................................................86
9.5.3 Pump tube replacing ................................................................................................86
9.6 As Needed Maintenance....................................................................................................90
9.6.1 Checking the Mark Position of Microscope .............................................................90
9.6.2 Replacing the microscope lamp ..............................................................................90
9.6.3 Replacing the Counting Chamber ...........................................................................92
9.6.4 Dredging Sampling needle ......................................................................................95
9.7 Data Backup/Restore .........................................................................................................95
9.7.1Data Backup .............................................................................................................96
9.7.2 Data Restore ............................................................................................................96
9.8 Troubleshooting .................................................................................................................97
9.8.1 Shutting Down the Instrument .................................................................................97
9.8.2 Restarting the Instrument ........................................................................................97
9.8.3 Error Messages .......................................................................................................97
10 APPENDIX ...............................................................................................................................107
10.1 Barcode Match ...............................................................................................................107
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AVE-772 Introduction
1 INTRODUCTION
1.1 Intended Use

The AVE-772 Integrated Urinalysis Analyzer is combination of Urine Formed Elements
Analyzer and Urine Analyzer. The urine microscopy results can also be combined with urine
chemistry results in an integrated report.The system is designed specifically for professional
use, as an In Vitro Diagnostic (IVD) device used by qualified physicians and laboratory staff.
The system is intended for use in screening to assist diagnosis for urinary system diseases,
kidney diseases, circulatory system diseases or infectious diseases.
1.2 How to use the User Manual

The User Manual is intended to explain the instrument operations in detail, to be a
training guide for new users and to be a troubleshooting reference manual.
This manual is designed for anyone who will operate the analyzer and must have a
thorough knowledge of it. It also specifically references information concerning analyzer
components, operation, theory, utility and performance. The manual is also loaded onto the
instrument computer and can be accessed by clicking the Help menu after entering the Main
Screen.
1.3 Precautions and Warnings Symbols – Definitions

The User Manual includes information and warnings that must be observed by the user to
ensure a safe operation of the system.
1.3.1 Cautions
CAUTION: Identifies situations that could lead to an accident or equipment failure.
CAUTION: Identifies electrical caution! Unplug before handling.
CAUTION: Identifies laser light. A laser is used to read the barcodes only. Protect
eyes from the laser light.
1.3.2 Warnings
WARNING: Identifies potentially hazardous situations that could result in serious
injury to laboratory personnel.
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1.3.3 Biological Warnings
Identifies potential biological hazards. Please be cautious when using, cleaning or

maintain the equipment and take appropriate protective measures.
1.4 Symbols
The following is a list of symbols used on the nameplate, product packaging or within the
user manual and their corresponding meaning.
No. Symbol Description Location
1 Cautions User manual, nameplate
2 Cautions User manual, instrument
5 Warning User manual
User manual, instrument,

6 Biological hazards
nameplate
Earth terminal, the terminal
7 Instrument
must be connected firmly
8 Power on User manual, instrument
9 Power off User manual, instrument
Nameplate, product
10 Date of manufacture
packaging
In vitro diagnostic medical
11 Nameplate
device
Nameplate, product
12 Manufacturer
packaging
Authorised representative in Nameplate, product
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the European Community packaging
Nameplate, product
14 CE Mark
packaging
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No. Symbol Description Location

Nameplate, product
15 Consult instructions for use
packaging
16 Fragile Product packaging
Do not use when package

17 Product packaging
damaged
18 Stacking limits Product packaging
19 Ban rolling Product packaging
20 Upward Product packaging
21 Keep dry Product packaging
1.5 Contact Information

AVE SCIENCE & TECHNOLOGY CO., LTD.
Building B6, Luguyuyuan, No.27 Wenxuan Road
Changsha Hi-tech Industrial Development Zone,
Hunan 410205, P.R.China
Tel: +86-731-8892 5308
Fax: +86-731-8890 7046
Website: http://www.c-ave.com/en.html
1.6 Limitation of Liability

AVE shall not be liable for any loss of use, revenue or anticipated profits, or for any
consequential or incidental damages resulting from the sale or use of the products.
1.7 Use of Third Party Computer Products

AVE does not recommend that the computers used as a functional part of the AVE-772 be
employed for performing any software or hardware based applications other than those
specifically furnished to operate and support the system, or those recommended and offered
by AVE specifically as accessories or enhancements for the system. No other third party
application software should be installed in these computers in addition to those provided or
recommended by AVE, without the expressed approval of AVE Technical Service, in order to
avoid potential performance and reliability problems which can result from incompatibility
factors, errors in use of such software, or software-based “viruses.”
Installation of such third party software, or non-approved electronic cards or other devices,
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without advance approval by AVE may affect the terms of or void any warranty otherwise in
effect, covering AVE-772 supplied software and hardware on the computers and the overall
performance and reliability of the entire system.
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AVE-772 System description
2 SYSTEM DESCRIPTION
2.1 Operation Theory

The user puts the test tube containing urine sample in the provided rack and place the
rack on the automatic sampling module. The device will detect the rack and automatically
forward it to the sampling position. If the sample is barcoded, the analyzer will read the
barcode, blend the sample thoroughly and aspirate it via the sampling needle into the
counting chamber for testing. The microcamera controlled by the host device scans the
sample in the counting chamber through enlarging the formed elements by microscope and
takes images for analysis and processing. The chemistry part will complete the separation of
chemistry strip and sampling automatically, also the test strip will be conveyed to the
chemistry components to do automatic chemistry analysis. The chemistry part uses CCD
camera to to detect the color changes of urine test strip, calculate the related items’
concentration, and output the semi-quantitative results.
During microscopy, the instrument regulates the focus automatically for different view
field to capture the cell images clearly. Then it recognizes, classifies and counts the particles
in each image with auto-recognition software which works with neural network algorithm.
Each image can be classified into one of 9 categories: normal RBCs, abnormal RBCs, WBCs,
Casts, Epitheliums, Crystals, Fungi, Mucus and others according to advance trained
recognition template.
The instrument simulates manual microscopy using fully-automated machine visual
technology. It can automatically select special analysis processes for different samples
according to the preset parameters. First the instrument screens for negative samples with
low magnification scanning before samples in the counting chamber have completely
precipitated to significantly increase the analysis speed. For positive samples, the instrument
performs LP (low powerfield) targeting and HP (high powerfield) tracking for distinguishing of
cells in order to improve the processing speed and accuracy. These steps ensure the
detection of cells in the un-centrifuged specimens. All the artificial intelligence functions
including data access, analysis and integration are achieved through the software. The
complete Urinalysis report including the images may be printed via a laser printer.
2.2 Performance and Technical Index

Microscopy: All observed particles under the miscscopy view can be detected and
confirmed;
Physical: Conductivity, color and turbidity informationl can be detected;
Chemical: Automatically analyzing urine chemical items;
Self-learning Function: Recognition accuracy can be improved through continuously traning;
Anaysis Throughput: ≥50/hr
Detection Rate：≥98%(at concentration level of 5 cell/µl)；
Accuracy Deviation (with Verification) ：≤5%;
Precision rate bias：The accurate rate of chemistry result match up with the random urine
test strip：Random urine test strip test results of all test items labeled with the corresponding
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value of the difference with the reference solution to not more than one order of magnitude.
Positive results do not appear negative, negative results do not appear positive;
Repeatability: See the table below
Concentration ( cell/µl) 20～100 500～1000 5000
CV(%) ≤20 ≤12 ≤8
Cross Contamination(Microscopy)：≤1 cell/µl at concentration of 4600～5400 cell /µl; ≤2
cell/µl at concentration of 9200～10800 cell/µl;
Cross contamination(Chemistry)：Using negative specimen to do cross test with the 3+
positive quality control, the negative sample will not show positive result;
Test Sample Volum：3ml；
Sampling and Cleaning： Automatical sampling,homogenizing,filling and cleaning;
Counting Chamber: Each channel work by time-share, and automatically cleaning,
constraint cleaning and maintenance;
Network Function：Information can be shareable through network;
Expansion Tests：Expansion tests can be carred out, such as Hydrothorax, Cerebrospinal
Fluid etc;
Report Form：Comprehensive report (including chemical, physical, particles counting and
morphological images, etc.);
Memory Capacity：≥20,000;
Microscopy Control：Five-axis automatic control, control precision is less than 0.5µm，with
automatic focus.
Quality Control Function ： Record quality control parameter, do quality control result
statistics, display and print quality control statistical graph;
2.3 System Components

The AVE-766 analyzer is a bench top instrument, which consists of Host, PC Module,
Peripherals (including display, mouse and keyboard) and other accessories(such as printer
etc.).
Host consists of the following four modules:
• Microscopy Module
• Sampling Module
• Automatic Sampler
• Strip Feeder
• Strip Loader
• Chemistry Analyzing module
2.3.1 System Appearance

Appearance of the system is as follows:
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3 4
Fig.2-1 Front view of the system
1.Microscope observation window;2.AVE-772; 3.Automatic sampler; 4.Barcode reader;
Fig.2-2 Back view of the system

1.Cooling fan; 2.Power socket(with fuse); 3.Microscopy CCD port; 4.USB port; 5.Chemistry CCD port;
6.COM port 1; 7.COM port 2; 8.Waste sensor port; 9.Reagent sensor port;
10.Cleanser I connector; 11.Buffer connector;
12.Cleanser II connector; 13.Waste connector;
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1
2
Fig.2-3 Left side view of the system
1.Main power switch; 2.Automatic sampler power switch;
2.3.2 AVE-772’s structure

The analyzer’s structure is given in the following flowchart:
Fig.2-4 AVE-772’s structure
2.4 Software Functions

The analyzing software is a set of microscopic analysis and urine chemistry analysis in
one, it has the following functions:
•Automatic microscopy function: Automatically detects whether a sample exists,
automatically moves and extracts a sample, and captures images automatically.
•Chemistry analysis function: Automatically capture the image of test strip, identify the
image, then provide the chemistry results.
• Automatic recognition function: Classifies microscopic particles of each sample using
the world's leading machine visual technology.
• Manual review function: The software provides editing capabilities for reclassification of
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the particles as decided by the user.

• Barcode scan matching and chemical negative screening function.
• Quality control function: Provides quality control test.
• Data inquiry and report printing function.
• Data backup and restoring function
2.4.1 Main Programme Screen

After booting, the user can enter the main programme screen as shown in below through
log-in. The function menu bar is located at the top of the screen, clicking on the different
drop-down menus enables entering each function of the operation interface. The second row
shows shortcut keys to the menu.
Function menu bar
Shortcut keys area
Fig. 2-5 Main Programme Screen

Function menu bar includes the following function main menus:
• Daily Operation (Operation)
• Management
• Quality Control (QC)
• Data Inquiry (Data)
• Help
The common shortcut keys are located in the shortcut key area and are convenient for daily
use, including microscopic examination, verification, urine history verification, urine inquiries, user
management, data backup, quit the system buttons, as shown in Figure 2-6. Clicking on the
corresponding function key enable the corresponding operation or entering the appropriate
function operation interface.
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Microscopy and
Quit
Verification
Urine History
Verification Urine Inquiry User Management Data Backup
Fig. 2-6 Shortcut keys area
2.4.1.1 Daily Operation Main Menu
Fig.2-7 Operation main menu

Daily operation main menu includes:
Submenu Function
Microscopy Open microscopy window
Password Change user password
Re-login Switch user
Quit Exit software
2.4.1.2 Management Main Menu
Fig. 2-8 Management main menu

Management Main Menu includes:
Submenu Function
Microscopy Setting Set the urine test items, urine reference values and
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cross-reference table.
Chemistry Setting Set Chemistry Items and Chemistry Protocol
Set the patient’s information of import, printing and
Patient Info. Setting
displaying
Mark Color Setting Set the target display color
Print Format Set report template
Print Setting Set the print and preview parameters
Adjustment Adjust parameters of the analyzer
User Set Create user, modify user password
Backup Backup and restore database and settings
2.4.1.3 Quality Control Main Menu
Fig.2-9 QC main menu

Quality Control Main Menu includes:
Submenu Function
Urine QC Urine accuracy or sensitivity QC screen
Chemistry QC Urine chemistry QC screen
2.4.1.4 Data Main Menu
Fig.2-10 Data main menu

Data Inquiry Main Menu includes:
Submenu Function
Review the data (including urine/QC inquiry, statistics and
Urine Data
history verification)
Tube Records Review rack/tube records
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2.4.1.5 Help Main Menu
Fig.2-11 Help main menu

Help Main Menu includes:
Submenu Function
Help Open the electronic user manual
About About the analyzer
2.4.2 Microscopy and Verification Screen

After entering the Main Screen, to enter the Microscopy and Verification (Microscopy)
screen by one of the following ways:
1: Click “Operation”-“Microscopy” to enter the Microscopy Screen.
2: Click the “Microscopy and Verification” icon in the Shortcut keys area to enter the
Microscopy Screen.
Fig. 2-12 Enter Microscopy Screen way 1
Fig.2-13 Enter Microscopy Screen way 2
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Channel Status Bar

View Switch Area
Online Instrument Special

Status Adjustment Specimens
Area Area Handling
Area
Patient Microscopic Field

Informati
on Area
Specimen ID Area
Fig. 2-14 Microscopy Screen
2.4.2.1 Urine Real-time Monitoring Screen

As shown in Fig.2-14, the microscopy screen consists mainly of the Online status
Area,the Instrument Adjustment Area, the Special Specimens Handling Area, the Microscopic
Field, the Specimen ID Area, the Patient Information Area, the View Switch Area and the
Channel Status Bar. Their respective functions are
Online Status Area: Display online status information of each part of the instrument as
well as convert its start and stop working state.
Instrument Adjustment Area：Common instrument functions can be adjusted here.
Special Specimens Handling Area：Some handlings on special specimens (QC, STAT,
reinspection, etc.) can be operated here.
Microscopic Field：Display microscopic images.
Patient Information Area：Display the detail information of the selected patient samples
as well as edit and modify it.
Specimen ID Area：This area includes both urine routine and special microscopy data
lists which display the data of tested specimen, including Specimen ID, barcode, microscopy
ID, chemistry ID and sample status. Double-clicking on the selected specimen ID opens its
microscopy screen.
View Switch Area：Mainly switch between real-time monitoring screen, urine microscopy
screen, and daily maintenance settings.
Their functions are:
A. Online Status Area:
“Start” button: A dynamic button whose initial status is “Begin microscopy”, will turn grey
after being clicked. The “Pause” button can also be clicked to achieve conversion between
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the two states. Functions: The instrument will fulfill the current sample analyzing by clicking
“Start” . This operation is for normal microscopy. The instrument will stop working temporarily
on clicking “Pause” and turn to maintenance state. The user can conduct a relevant quick
maintenance and resume its work by clicking on “Continue”. It will be converted to the specific
channel by clicking the corresponding one and begin sampling, focusing and washing.
“Stop”: Stop testing normally. The system will stop working after finishing the sample,
which is currently tested in the cell by clicking on “Stop”. The next sample on the current rack
will not be tested.
“Forced”: Forced to stop testing. The system will stop testing both the sample in the cell
and the next one on the current rack by clicking on “Forced”.
B. Instrument Adjustment Area:

“↑ / ↓”：Be used for focus fine-tuning. “↑”in the first row means each click equals a focus of
the microscope upward 5 steps, while “↓” in the same row means each click downward 5
steps. “↑” in the second row means each click 10 steps upward and “↓” 10 steps downward.
“HP / LP”: Realize the microscope HP/LP conversion.
“Save”: If you need to save the focus you’ve adjusted, just click this button.
“Sample”：Sample through a single selected channel.
“Wash”: Washing of a single selected channel (Cleanser A).
“Spe-Wash”: Compulsorily washing a single selected channel (First the channel is soaked
with Cleanser B, then Cleanser A is used for cleaning).
“Wash+”: Wash a single selected channel with double volumn cleanser (Cleanser A).
“Manual”: Allow for manual capturing of microscope pictures.
“Pause”: Click this button will pause the instrument, while performing any regulation in
Instrument Adjustment Area.
C. Special Specimens Handling Area:

“Insert” button: Manually insert the type and ID of the next special specimen, mainly for a
special specimen into the consecutive ones.
“Insert at”: Enter the insertion position of a special specimen into the consecutive ones.
System defaults the test of the special sample in the position.
D. View Switch Area:

“Microscopy”: Switch to the real-time monitoring screen of testing.
“Verification”: Start report review procedure. The user can perform the manual verification
of the microscopy results here for the final examination report.
“Tools”: Click this button to pop-up a drop-down menu on daily maintenance and settings,
choose any of the options to enter the corresponding screen.
E. Patient Information Area:

“Print” Print the specified sample results report.
“Prview” Preview the specified sample results report.
“Batch” Print the specified batch of samples results report.
“Save” Save the recorded or modified information of the patients.
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2.4.2.2 Urine Microscope and Verification Screen

As shown in Fig.2-15 select “Verification” button, then the corresponding view is Urine
Microscope and Verification Screen .
Fig. 2-15 Microscopy and Verification Screen

The verification screen consists mainly of the Urine Information List, the Patient
Information Field, the Current Cells Couting , the Chemistry Data, the Function Key, the
Frame and the Image Display.Their respective functions are:
“Urine Information List”: Display the information about samples being microscoped.
“Patient Information”: Display the information about the patient and verify or modify the
given information.
“Frame”: Show the captured frames of the testing sample and the selected image’s frame
number.
“Current Cell Counting”: Display the amount of items in current sample tested under HP/LP.
“Chemistry Data”: Display the real time received chemistry data.
“Functional Key”: Include some common functional buttons for verification, such as “Modify”,
“Previous”, “Next”, “Zoom”, “Print”, “Preview”, “Batch Print”, “Save” and “X”.
“Image display”: Display the images of the current sample captured under HP or LP.
A. Functional Buttons
“Modify”：Clear out the counting data and cell type of the current picture and change to
another.
“Previou”: Select the previous sample.
“Next”: Select the next sample.
“Zoom”: Enlarge or diminish the selected pictures in the image showing field.
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“Print”: Print the selected information report.

“LP/HP”: This button is a two-way button. LP/HP pictures can be selected through pressing it
alternately.
“Preview” :Preview the selected information report.
“X”: Quit the “Urine Verification” screen.
“Save”: One for keeping the patient’s information and another for keeping results and
modifying the data.
B. Image display field

16 frames of the image captured are displayed here. The pictures can be divided into LP
and HP. If user select “LP” on the left of the “Current Cell Counting” field, then the pictures
captured under LP field appear. If select “HP” on the right, then the pictures under HP field
appear. Double click on any picture displayed in this field will enlarge the picture to a full-view.
Double click on the picture again will diminish it again.
C. Chemistry Data
Click the “▲”or “▼”button to preview the chemistry data received.
D. Current Cell Counting field

Detail information about the testing items are displayed in this field. The information given
are: “Result”, “Frame” and “Total”. Data in the “Frame” column are the data of the current
pictures captured; “Total” shows the total of the item tested under LP or HP.
E. Frame
The frame information of the current sample appears here.
20
AVE-772 Installation
3 INSTALLATION
3.1 Summary
Anyone who is not trained by AVE or authorized distributors of AVE may cause damage
to the analyzer when opening the box or installing the instrument. Therefore do not open the
box or install the instrument without an authorized technician being present.
On receiving the analyzer, please check the package carefully to see whether there is a
physical damage. If there is any, please notify your local distributor immediately.
3.2 Installation Requirements
3.2.1 Space
AVE-772 Integrated Urinalysis Analyzer (with its mainframe size of
825(L)x648(W)x550(H) mm) is a tabletop unit requiring free space on a countertop of
approximately 250x120 cm, with vertical clearance of at least 30 cm.
3.2.2 Power
Voltage Frequency Input power Fuse
AVE-772 100-240V 50/60Hz ≤650VA T3.15A 250V Φ5×20
CAUTION: UPS no less than 1500VA or voltage regulator are recommended for the
whole system operation if voltage is not stable or power outage is regular.
CAUTION: The equipment inside fixed covered areas of the instrument are not user
serviceable and may present electrical shock hazards.
WARNING: For continued protection against risk of fire and hazard, replace only with
the same type and rating fuse.
WARNING: Equipment requires connection to protective earth for safety reasons.
WARNING: The instrument’s main supply inlet is being used as the mains
disconnect device.
3.2.3 Environmental requirements

The instrument is suggested to be installed in the environment as:
• Normal working temperature: 15°C – 30°C
• Relative humidity：30% - 70%；
21
• Atmospheric pressure：500 - 1060hpa；

• Do not get close to strong electromagnetic source as not to affect the normal
operation of equipment.
• Keep the environment as clean as possible. Keep it free from mechanical vibration,
noise and power interruptions.
• Avoid direct sunlight or exposure to heat or wind.
3.2.4 Installation
The AVE-772 Urinalysis System has to be installed by an authorized technician.
3.3 System Connection

The authorized technician will complete the following connections when installing the
analyzer.
The system connects with other peripherals as shown in Figure 3-1. Ensure connecting in
place and firmly.
Fig.3-1 AVE-772 and its peripheral connections
3.4 Pipeline connection of the analyzer
Warning: User has the duty to comply with the relevant regulations set by the region
and country about the treatment and discard of expired reagents and wastes.
Warning: Reagents may cause some irritant to skin and eyes. User should always
comply with the safety operation of laboratory and wear personal protective equipment (such
as protective clothing, gloves, etc.) when coming contact with reagents.
Caution: Do not place reagent containers on the Analyzer

The system is connected with reagent bottles as shown in Figure 3-2. Ensure connecting
in place and firmly.
22
Fig.3-2 AVE-772 reagent connections
3.5 Power-cable connection

The main power-cable of AVE-772 connects the input power-outlet on the rear-side and the
power distribution board special for the system. Make sure that the power of AVE-772 and the
power distribution board is off before the connection is completed .Complete the cable connection
of the monitor and printer.
23
AVE-772 Setup
4 SETUP
The analyzer settings are already set by the factory and the screen you see after the
initial boot is set on system default. In order to meet the individual demands, some
parameters of the analyzer can be modified. Since the settings will directly affect the normal
use of the instrument, an access control has been set up for the user to ensure the safety of
the equipment.
There are two modes of use and appropriate rights: The "user" can perform clinical
urinalysis in daily use, but can not access "Management" main menu. In addition to the rights
owned by the "User", the "Administrator" can modify specific parameters on the
"Management" main menu.
CAUTION: The chapter is for “Administrator” only.
CAUTION：Changing of the factory parameter settings could affect the normal use of
the instrument , if it’s necessary, please read the user’s manual first or contact manufacture.
4.1 User Set
4.1.1 User login

You can enter the user set through the following two ways on the system software main
screen.
1. Click “Management”-User Set”.
2. Click the “User Set” icon in the Shortcut keys area.
Fig.4-1 User set entry mode 1
24
AVE-772 Setup
User Set
Fig.4-2 User set entry mode 2
Fig.4-3 “User Set” screen
4.1.2 User Set Operations

Operations as adding a new user, deleting a user and modifying a user can be performed
on the “User Set” screen.
Adding a new user:
a. Type the new user identification in the “User” field.
b. Type the new user password in the “Password” field.
c. Click drop-down menu in the “level” field to select the user level
d. Click “Add” button.
Delete a user:
a. Select the user to be deleted.
b. Click the Delete button.
Modify a user
a. Select the user to be modified.
b. Alter the user identification in the “User” field as needed.
c. Alter the user password in the “Password” field as needed.
d. Click drop-down menu in the “Level” field to alter the user level as needed.
e. Click “Save”.
4.1.3 Password Modification

1. Click “Management” in system main menu, select “User Set” in the drop-down
submenu as shown in Fig.4-1. The “User Set” application screen (as shown in Fig.4-3) open.
Following the instructions of “Modifying a user” in 4.1.2 the administrator can modify any
25
AVE-772 Setup
user’s password.
2. Click the daily operation main menu, select “Password” in the drop-down submenu as
shown in Fig.4-4. The “Password” modifying window will open. The password of the login
user can be modified in this window. This is available for all users.
Fig.4-4 Password Modification
Fig.4-5 Password Modification window
4.2 Microscopy Setting

Click “Management” in system main menu, select “Microscopy Setting” in the
drop-down submenu, the three secondary submenus “Item Setting” , “Reference Values”
and “Comparison List” will open.
Fig.4-6 Urine Microscopy Setting menu
26
AVE-772 Setup
4.2.1 Item Setting

On this screen selecting “Microscopy” in the “Item Type” will allow to add, delete, alter
and sort the items.
Fig.4-7 Urine Item (Setting) screen
CAUTION：It is not recommended to change the parameters in this interface, if any,
please contact technicians or manufacture.
4.2.1.1 Urine Item (Setting) Screen

Parameter: the type of formed element
Display Name: the item name as displayed in the microscopy verification list
Sen. Para. (Senior Parameter): the senior parameter of the item. The parameters are now
available RBC, WBC, Crystal, Cast, Epithelium, Yeast, Bacterium, Parasite, Sperm and
others. Senior parameter setting will default a new added item but not classify it to a senior
parameter with the same name otherwise lead to a logical error.
Unit: the unit of the item
Reference: the normal reference value. The reference values of RBC, WBC, Crystal, Cast,
Epithelium and Yeast shouldn’t be set here but being set in Urine Micro Reference interface.
See 4.2.2 for details.
Display type: Set the item name displayed in HP/LP Microscopy Verification List and right
click menu. Both means the item displays under both HP & LP, LP display means the item
displays only under LP and HP display means the item displays only under HP; Neither
means the item will not display under HP or LP.
Print: Whether to print this item or not.
Identify: Whether to identify the item automatically or not. It is a default setting, modification is
27
AVE-772 Setup
not recommended. For a new added item choose “No”.

QC: Whether to run a morphological QC. It is a default setting, modification is not
recommended. For a new added item choose “No”.
Identifier: the ID code of the item. It is a default setting, modification is not recommended .
The ID code of a new added item should not exist.
Remarks: Add notes for the item.
Delete: Delete the selected parameters.
Up/Down: Move up/down the selected parameters for sorting.
Quit: Close the modifying interface.
4.2.1.2 Add Urine Items
1: Choose a blank row, enter the appropriate parameter, display name, unit, reference (if any);
set display type, print or not; choose No for Identify and QC, set corresponding identification
as “-1”.
2: The new added item will be saved automatically after enter.
4.2.1.3 Delete Urine Items
1: Select the urine item in Urine Item interface. Caution: only items added as described
under 4.2.1.2 can be deleted. Systems items cannot be deleted.
2: Click the Delete button to confirm the operation.
4.2.1.4 Move Urine Items
1: Select the urine item in Urine Item interface.
2: Click the Up or Down button to change the location of the item in the list.
4.2.2 Reference Values

On this screen the user can set gender, maximum age and minimum age for the
reference value of RBC, WBC, Crystal, Yeast, Cast and Epithelium.
Fig.4-8 Reference Value setting screen

Reference Value of various age range and gender on this screen can be added, deleted
and altered.
28
AVE-772 Setup
Add operations:
1. Choose Female or Male in the Gender box.
2. Select a blank row, enter maximum age, minimum age and the corresponding reference
values of RBC, WBC, Crystal, Yeast, Cast and Epithelium.
3. Click Save button and click “OK” in the pop-up window to validate the adding.
Delete operations:
2. Select the row of reference values of age range to be deleted.
3. Click Delete then Save button, and click OK in the pop-up window to confirm.
Modify operations:
2. Select the parameter to be altered.
3. Enter a new corresponding parameter,
4. Click Save button and click OK in the pop-up window to confirm.
5. Click Quit to exit.
4.2.3 Comparison List

By setting the quantitative and qualitative data comparison of urine formed elements, the
Urine Comparison Table achieves the automatically comparison between sample’s strips and
sediment results, analyzing whether the two results are matched or not under clinical
diagnosis standard and whether reminding current user to verify the results or not. It is a
default setting, modification is not recommended. If any, please operate in strict accordance
with clinical requirements. As shown in Fig.4-6, select the secondary submenu Comparison
List to enter the Comparison List interface.
Fig.4-9 Urine Comparison Table setting screen

Each comparison parameter on the Urine Comparison Table setting screen can be added,
deleted and altered.
Add operations:
29
AVE-772 Setup
1. Select an item in Item box.

2. Enter actual data and report data respectively.
3. Click Save to confirm.
Delete operations:
2. Delete the comparison value row.
3. Click Delete to confirm.
Modify operations:
2. Select the value to be altered in the comparison table.
3. Enter a new corresponding comparison value.
4. Click Save button and click OK to confirm.
4.3 Chemistry Setting
4.3.1 Chemistry Setting

Select “Chemistry setting” in “Management” menu to pop up two secondary submenu
“Chemistry Item” and “Chemistry Protocol”. Select the “Chemistry Item” to pop up
Chemistry Setting window as shown in Fig.4-11.
Fig.4-10 Chemistry Setting menu

Selecting “Chemistry Item”in “Item Type” box from the setting window can perform all
the settings.
30
AVE-772 Setup
Fig. 4-11 Chemistry Setting screen
4.3.1.1 Chemistry Setting screen

Item Instructions:
Parameter: Chemistry Item.
Abbreviation: The abbreviation of external chemistry analyzer item.
Unit: The unit of external chemistry analyzer item.
Reference: Normal reference value.
Judge: Whether to compare the item with microscopy result. 1 stands for “Judge” and 2 for
“Not Judge”. It is a default setting, modification is not recommended.
Print or not:Whether to print the item or not. 1 stands for “Print” and 2 for “Not Print”.
Remark: Add notes for the item.
Delete: Delete the selected parameter.
Save: Save newly added or modified parameter.
Up/Down: Move up or down the selected parameters for sorting.
Quit: Quit the setting screen.
4.3.1.2 Adding Chemistry Item
1. Select an adding location on Chemistry Setting screen
2. Enter the corresponding parameters into the blank row.
3. Click the Save button and “OK” in the pop-up window to validate the adding.
4.3.1.3 Deleting Chemistry Item
1. Select an item to be deleted on Chemistry Setting screen
2. Click the Delete button to delete the item selected.
3. Click the Save button and “OK” in the pop-up window to validate the deleting.
4.3.1.4 Moving Chemistry Item
1. Select the item to be moved on Chemistry Setting screen
31
AVE-772 Setup
2. Click the Up or Down button to move the item to where you want.
3. Click the Save button and “OK” in the pop-up window to validate the deleting.
Click the Quit button after all the operations fulfilled to exit the Chemistry Setting screen.
4.3.2 Chemistry Protocol

Select the “Chemistry Protocol” in “Management” menu to pop up the setting screen
shown in Fig.4-12. It is used for AVE-772 to set communication protocol with chemistry
analyzers.
Fig. 4-12 Chemistry Protocol screen
4.3.2.1 Chemistry Messaging parameter setting

Instructions:
New:New added protocol name.
Protocol: Existing protocol name. In General, chemistry analyzers made in China can use
“common chemistry analyzer”interface program.
Items: This is used for the converting of external chemical data into the corresponding
software report data. For example, if the chemical result for urine occult blood is “Neg” and we
set the report data as“-”, the software can convert it into“-”automatically.
Add: Add a new protocol.
Delete: Delete the corresponding protocol.
Save(upper left): Save the protocol newly added, deleted or modified.
Save(lower left): Use the current selected protocol.
Quit: Quit the screen.
Test: Test the communication of the chemistry analyzer connected and transmission of the
results.
Clear: Clear the results.
4.3.2.2 Adding Existing Chemistry Protocol
1.Connect the chemistry analyzer to AVE-772 correctly.
2.Select the corresponding chemistry analyzer in “Protocol”, click “Save” (upper left).
32
AVE-772 Setup
3.Select the corresponding chemistry analyzer in “Save”, click “Save” (lower left) to
accomplish adding.
4.3.2.3 Adding New Chemistry Protocol
1.Connect the chemistry analyzer to AVE-772 correctly.
2.Enter a new chemistry protocol in “New”, click “Add”.
3.Select the corresponding chemistry analyzer in “Protocol”, click “Test”. Choose any data of
the chemistry analyzer and transfer it to the test screen, then fill out the “Start”, “End”, “ID”,
“Y”(year), “M”(month), “D”(date). Click “Save” (upper left).
4.Select the corresponding chemistry analyzer in “Save”, click “Save” (bottom left) to
accomplish adding.
4.3.2.4 Adding Comparison Data
1.Select the corresponding item in “Items”.
2. According to the actual data of the chemistry analyzer, click the blank in “Actual data” and
input the information. Click the blank in “Report data” and input the corresponding information.
Click “Save” (upper right) and click “OK” in the pop-up window to accomplish adding.
3. Select other items and fulfill all the parameter settings referring to step 2.Click Quit to exit.
4.4 Patient Info. Setting

As shown in below, Select Patient Info. Setting in Management menu to pop up the three
secondary submenus Input Patient Info., Print Patient Info. and Show Patient Info.
Fig.4-13 Urine Item Setting interface
4.4.1 Input Patient Info.

As shown in Fig.4-13, Select Input Patient Info.. Patient information can be pre-entered
into the drop-down box as shown in below.
33
AVE-772 Setup
Fig.4-14 Input Patient Information interface

Explanation about some abbreviations
- Int. Med. (Internal Medicine)
- Ortho. Surgery (Orthopaedics Surgery)
- Urology (Urology Department)
- Andriatrics (Andriatrics Department)
- Gynecology (Gynecology Department)
- Obstetrics (Obstetrics Department)
- Resp. Med. (Respiratory Medicine)
parameters including department name, test purposes, clinical diagnosis, sample status,
submitter, checker, color and turbidity can be added, deleted, modified or moved on the
interface.
Add operations:
1. Select the type of the information in Info Type box.
2. Select a blank row in the information input area.
3. Click Save button to confirm.
Delete operations:
2. Select the content of the parameter in the information input area.
3. Click Delete button to confirm.
Modify operations:
2. Select the content of the parameter in the information input area.
3. Enter a new corresponding content of the parameter.
Move operations:
2. Select the content of the parameter in the content box.
3. Click Up / Down button to move the content.
34
AVE-772 Setup
4.4.2 Print Patient Info.

As shown in Fig.4-13, select Print Patient Info. Users can set whether the Patient
information item be printed or not, if ticked it will be printed, otherwise it will not be printed.
Click Save to confirm and Quit to exit.
Fig.4-15 Patient information printing interface

Inp-ID (Inpatient ID): If a patient must be hospitalized, they will be assigned a number, which we
call it inpatient ID.
Dept. (Department): The department that the patient belongs to.
Subm. D (submitted date): At which day when the sample is submitted to the laboratory.
Subm. T (submitted time): The time when the sample is submitted to the laboratory.
Samp. D (sample date): At which day the sample is examined by the operator.
Samp. T (sample time): The time when the sample is examined by the operator.
Subm. (submitted by): The clinician who submit this sample.
Conduct. (conductivity): The conductivity value of this sample.
Verified (verified by): The operator who verifies this sample.
4.4.3 Show Patient Info.

As shown in Fig.4-13, select Show Patient Info. Users can set whether the Patient
information item be displayed in the Patient Information Area of the Microscopy and Verification
screen or not, if ticked it will be displayed, otherwise it will not be displayed. Operations of
Microscopy Info Table are as same as above and the items will be shown at Specimen ID Area.
Click Save to confirm and Quit to exit.
35
AVE-772 Setup
Fig.4-16 Patient Information showing interface

Samp. ID (Sample ID): Sample ID
Diagn. (Diagnosis): Preliminary visual result
Turbid. (Turbidity): Turbidity of the sample
4.5 Mark Color Setting

Select Mark Color Setting in the Management menu, as shown in below.
Fig.4-17 Mark Color Setting interface

Operation steps:
1.Select a cell type in the Cell Type column.
2.Click the Color button to set the color.
3.Click the Save button to confirm.
4.Click the Quit button to exit.
Color default settings: Red(RBC);Blue(WBC);Black(Crystal);CyanGreen(Fungus);
Purple(Epithelium);Yellow(Cast)
36
AVE-772 Setup
CAUTION：If change the color setting, please set different cell types in different
colors in order to distinguish.
4.6 Print Format

Select Print Format in the Management menu, as shown in below.
Fig.4-18 Print Mode Setting interface

Operation steps:
1. Select Urine in Select area.
2. Select a printing format in the right box.
3. Select A4 Format/A5 Format button if needed.
4.Click the Quit button to exit.
4.7 Print Setting

Select Print Setting in the Management menu, as shown in below.
37
AVE-772 Setup
Fig.4-19 Print Parameters setting interface

Instructions:
General Setting
Hospital: Enter the hospital name. It will be displayed on the lab report.
Address: Enter the hospital address.
Remark: Enter notes, if necessary.
Microscopy Setting
Format: Select A4 or A5 format.
RT Print: Real time Print. Automatic print will be proceed immediately after negative
specimen being tested, if Yes is selected.
Print Image: Print images, if Yes is selected.
Image Number: Set the image quantity in a lab report. The maximum quantity is 6 for A4 and
3 for A5.
Print Neg: Whether to print the image of negative specimen.
Report Field: Set the microscopy result as semiquantitative result, such as /LPF, /HPF.
Print Item: Print the microscopy result as quantity or quality. Select both to show the
quantitative and qualitative results at the same time.
Morphology: Whether to print the morphological graphs. Morphological graphs will only be
shown if more than 3 RBCs identified.
Morph. Lower Limit: Set up the cutoff value that printing morphological images. It is
recommended to set the lower limit at 10.
Chemistry Setting
Report Format: Set chemistry report format. Select Separate Report to print chemistry
results only.
Format: Select A4 or A5 format for chemistry report.
4.8 Tools
Select Tools in Microscopy main interface, users can perform Test Mode Setting, Users
settings, Edit ID, Unusual, Gallery, Positive List, MagnGlass On/Off etc.
38
AVE-772 Setup
Fig.4-20 Tools interface

4.8.1 User Settings
Select User Settings in the drop down menu as shown in Fig.4-20, users can set the Input
Method, Cell Mark On or not, Cell Notice On or not by select or tick. Click Save to confirm
and Quit to exit as shown in below.
Fig.4-21 User Settings interface

Input Method：Select the default input-method.
Cell Mark On：If ticked, cells in Verification interface will be marked, otherwise not.
Cell Notice On：If ticked, cells in Verification interface will be noticed, otherwise not.
4.8.2 Unusual
Select Unusual in the drop down menu as shown in Fig.4-20, users can set Delay, Close
CH or not in the pop up interface as shown in below.
39
AVE-772 Setup
Fig.4-22 Unusual setting interface

Delay: Set the delay time after the chamber is filled in. Tick the options, enter the delay time
and click Save to confirm. Click Quit to exit, system will initialize the default settings.
Close CH: Tick to close each channel. After Quit , channel will remain close, if the channel
needs to be opened, please cancel the tick.
4.8.3 Gallery
Select Gallery in the drop down menu as shown in Fig.4-20, the Gallery includes various
kinds of formed elements pictures, such as RBC(HP/LP), WBC(HP/LP) , Cast(HP/LP),
Crystal(HP/LP), Epithelium(HP/LP) and Fungus(HP/LP), for users’ reference only. It is able to
ask our manufacture or technicians to add new pictures into the Gallery as shown in below.
40
AVE-772 Setup
Fig.4-23 Gallery interface

4.8.4 Positive List
This function is mainly used for Microscopy and Chemistry results automatic statistics
and printing and convenience for review.
The Microscopy Positive list displays those samples where any data of RBC, WBC, Cast,
Crystal and epithelium is positive. The Chemistry Positive list displays those sample where
any result of BLD, LEU and PRO is positive.
Fig. 4-24 Positive List

4.8.5 MagnGlass On
This function is used for open/close a magnifying glass, so that the picture could be
enlarging while performing verification. Click on the MagnGlass on, you can verify the picture
on the left of verification screen there the magnified images appead.
41
AVE-772 Setup
Fig.4-25 MagnGlass On interface

4.8.6 Test Mode Setting
Select Test Mode Setting in the drop down menu as shown in Fig.4-20, users can enter
the Test Mode Setting interface as shown in below. Click Save to confirm and Quit to exit.
Fig.4-26 Test Mode Setting interface

Micro+Chem：Perform microscopy and chemistry test for each sample.
Chem：Perform only chemistry test for each sample.
Micro：Perform only microscopy test for each sample.
It’s recommended to change the setting before test starts or the current samples have
finished.
42
AVE-772 Specimen processing
5 SPECIMEN PROCESSING
5.1 Power On
1. Turn on the UPS (if any).
2. Turn on the power distribution board (if any).
3. Turn on the power switches of printer, monitor, etc.
4. Turn on the main power switch(Green switch on slide, if it’s on, the yellow LED inside of the
Green switch will be lighted).
5. Turn on the auto-sampler’s power switch(Green switch on slide, if it’s on, the yellow LED
inside of the Green switch will be lighted).
6. Press the Power button of the PC Module to enter Windows OS.
5.2 Login
Double click the shortcut icon on desktop to display the login window shown in
Fig.5-1
Fig.5-1 Login screen

Type user name in the Name field, or select user name in drop-down menu.
Type user password in the Password field.
Click the Log in button to log into the main interface as shown in Fig.5-4.
If it’s a first time use, please set up parameters under the guidance of an authorized
technician. See Chapter 4 for details.
CAUTION: Ensure that the SQL Server in task bar is started before entering the
AVE software. If not, double click the icon as shown in Fig.5-2 to pop up the SQL Server
Manager interface as shown in Fig.5-3. Click the start / continue button to launch.
Start state: Stop state:

Fig.5-2 SQL Server status icons
43
Fig. 5-3 SQL Server window
Fig. 5-4 Main interface

User Switch
After users login, if it’s needed to switch to other user, please operate as following steps:
Select Re-login in the drop down menu of Operation in system main menu to pop up login
interface as shown in Fig.5-1.Select or enter a new user name and password to login again.
5.3 Daily Quality Control (Daily QC)

Daily QC should be run to ensure reliable results before sample analysis. See Chapter 6
Quality Control for details.
5.4 Specimen Preparation
CAUTION:Use only fresh urine specimens, as defined in NCCLS (National

Committee on Clinical Laboratory Standards) GP16-A3 protocol, Good Laboratory Practices
and the Laboratory’s Procedure Manuals.
44
CAUTION:Collect urine in clean and sterilized containers. If a specimen is not

tested within an hour after collection, cover the container tightly and store at 2-8°C. Make sure
the specimen back to room temperature before testing.
CAUTION: Mixing the specimen well before testing.
CAUTION :Do NOT add disinfector or cleanser into the specimen.
CAUTION:Keep specimens out of direct sunlight.
CAUTION:Do NOT centrifuge the urine specimens.

5.4.1 Temperature
Specimens should be tested at room temperature. If the temperature is beyond the room
temperature range, make sure that all refrigerated specimens back to room temperature
before testing.
5.4.2 Specimen Volume

Specimen volume for AVE-772 Integrated Urinalysis Analyzer should be minimum 4ml
per test, if users want test the specimen more than one time, please prepare enough volume.
Biological WARNINGS: Specimen, QC material and waste are potential

biological hazards. User contacting with related items in the laboratory should comply with the
safety requirements and wear personal protective equipments(such as laboratory protective
clothing, gloves, etc.)
5.4.3 Sample Tube
Φ16 mm x 100 mm (L), glass or plastic round bottom tube.
5.4.4 Preparing Sample Racks
CAUTION：Please use the racks special for AVE-772.
CAUTION：Make sure the barcode labels face to barcode scanner.
1. Apply barcode labels to sample tubes, make sure that the barcode (not the barcode label)
is ½ inch (about 12.7 mm) below the top edge of the tube(Φ16 x 100 mm).
2. Prepare at least 4ml well-mixed native specimens into sample tube.
3. Put the tubes into the sample rack correctly and in order.
4. Launch the sample rack on the Ready Area of the sampler.
5. Mark the tubes as shown in Figure 5-5.
45
Fig. 5-5 Sample Rack

Barcodes accepted by the scanner are: Code 128, Code 39, Codabar and Interleaved 2 of 5 (I
2 of 5).
CAUTION: Make sure the barcode labels are properly oriented in the rack. The
sample tubes must be placed straight and resting in the middle of the grommets located in the
base of the rack.
5.5 Urine test strip preparation

AVE-772 analyzer can only use the urine test strip produced by AVE company.
Please place the test strips into the instrument following below steps before sample test.
Step 1: Check whether the quality guarantee period in test strip is within the valid date. If
it is not, do not use it.
Step 2: Open the front panel of the instrument, then open the the test strip can, then
open the test strip tank, put the test strips into the test strip tank according to the direction as
shown in figure 5-6.
Step 3: Put the strip can’s cap into the strip tank door’s dryer hole, and close the tank
door. Then close the front panel of the instrument.
Fig.5-6 Put in the test strip
46
CAUTION: Each time to put a new can of test strips, make sure to replace the can’s
cap into the strip tank door’s dryer hole.
5.6 Running Samples

There are two ways to enter the Microscopy interface as shown in Fig.5-3.
1.Select Microscopy in the drop down menu of Operation on the system main menu as
shown in Fig.5-7.
2.Click Microscopy shortcut icon in the shortcut keys area as shown in Fig.5-8.
Fig.5-7 Entry Microscopy screen way 1
Fig.5-8 Entry Microscopy screen way 2

As soon as Microscopy interface is entered, the system will perform auto initialization and
auto focusing. It will take approximate 30 seconds to finalize initialization.
47
Fig. 5-9 Initialization

Select Test Mode Setting in the Tools menu, users can enter the Test Mode Setting
interface as shown in below. It’s recommended to change the setting before test starts or the
current samples have finished.
Fig.5-10 Test Mode Setting interface
Fig. 5-11 Microscopy and Verification screen

Click the Start button on the Microscopy interface to achieve ready status, as shown in
Fig.5-11. Launch sample racks to the Ready Area of the sampler, microscopy testing will
processed automatically.
CAUTION: Do NOT move the sample racks in Ready Area during testing.
CAUTION: No operations should be performed during the self-checking procedure.
WARNING: Don’t place your hand below the sampling position to avoid injuries by the
sampling needle.
Explanation about the sample status
48
For each testing result, the system will presents the status for this sample accordingly, the
user can define the further processing according to the status. In general, the status are:
 NE (Not Examined): The microscopy examination of this sample is not finalized yet.
 NV (Not Verified): The microscopy result of this sample is positive, or the strip result did
not coincide with microscopy result, further verification is recommended.
 V (Verified): The microscopy result of this sample is negative, or the strip result coincide
with microscopy result, it’s not necessary to perform further verification.
 R (Reported): This sample was already verified and printed out.
 NV! (Not Verified!): The microscopy result of this sample is positive, or the strip result did
not coincide with microscopy result, it’s NECESSARY to perform further verification.
 EV (Expert Verify): This sample was already verified by online expert. (only available for
remote connection mode)
 NS (No Sample): The specified volumn of this sample is not attained, thus it was not
examined. The user need to check the volumn and test this sample again.
5.7 Patient Data Entry

The software provides a patient data entry function. Users can type in the patient
information at any time even the analyzer is testing specimens. If the analyzer is already
connected to LIS, the way to type in patient information could be faster and easier, operation
steps are as following: Type in Inp.ID, if the Inp.ID is already existed in LIS system, system will
show all the information of the Inp.ID from LIS when users click other input box.
5.8 Dealing with Special Microscopy

AVE-772 provides emergency priority testing and repeated testing function.
5.8.1 STAT Test
STAT Test must perform at the emergency position.
Operation steps are as following:
1. Prepare the emergency sample.
2. Put the sample at emergency position and push it forward, wait until system pop up the
Insert STAT interface as shown in below.
3. Select Urine Micro in the Micro box, STAT in the Type box, Micro in the STAT box, type
in the sample ID in the Sample ID box. Sample ID and Sam-ID must be the same. Users
can only type in one sample ID at one time. In principle, the start number of emergency
samples must be larger than all the specimens numbers. The barcode of the emergency
sample must be typed in the Barcode box manually. Click the Insert button to add the
corresponding information into the Special Sample field.
4. Pull the emergency position back after the sampling is finished.
49
Fig.5-12 Insert STAT interface
5.8.2 Recheck
If it’s needed, users can perform Redo operation to repeat the Microcopy testing. Operation
steps are as following:
1.Prepare the emergency sample.
2.Put the sample at emergency position and push it forward, wait until system pop up the
Insert STAT interface as shown in Fig.5-13.
3. Select Urine Microscopy in Micro box, Redo in Type box and Urine Redo Item in Redo
box as shown in Fig.5-13.The numbering rules is that start with 3 and last three number is as
same as Sample ID’s last three number, for example, if the Sample ID is 0013, then the Redo
ID is 3013.
Fig.5-13 Insert STAT Redo

4.Click Insert button and the Redo number will show in Urine Special field .
5. Pull the emergency position back after the sampling is finished, the testing will be finished
automatically.
6.Complete the sample verification according to verification process. Click the Save button
and click OK to confirm as shown in Fig.5-14. If click OK to save and overwrite the former
result in Urine Special field, click Cancel that will not overwrite the former one and both
results will be kept.
50
Fig.5-14 Redo Overwrite interface
5.9 Verification operations
5.9.1 Basic operations

It is recommended to verify the result manually when the sample status are NV and
NV!.
Enter the Urine Verification interface and refer to the following:
LP Verification
1.Enter the Urine Verification interface, select a sample, click anywhere of the LP Current
Cell Counting to switch to LP pictures, double click to enlarge the picture.
2.Observe whether there are results in the Cast, Epithelium column among the Current Cell
Counting field or not. If not, Users need to observe the LP pictures to make sure there is no
cast or epithelium in any of the LP pictures.
Note:Particle in LP Current Cell Counting means all small targets located in LP, do NOT
change!
3. Click Next to check in order. Verify and mark what you see actually in the images by right
click and select the right type/name in the pop up menu. If the item type/name is changed, the
result numbers in the corresponding column will also be changed; If the item type/name is
canceled, the corresponding result numbers will also be decreased.
Fig. 5-15 LP verfication

4. Click Next to view all LP pictures of the sample in full-view and repeat step 2 and 3 until
finished.
HP Verification
5. Click anywhere of the HP Current Cell Counting to switch to HP pictures, double click to
51
enlarge the picture.

6.Observe whether there are numbers in the RBC, WBC, Crystal, Bacterium, Yeast, etc,
column among the Current Cell Counting field or not. If not, Users need to observe the HP
pictures to make sure there is no RBC, WBC, Crystal, Bacterium, Fungus, etc, in any of the
HP pictures.
7. Click Next to check in order. Verify and mark what you see actually in the images by right
click and select the right type/name in the pop up menu. If the item type/name is changed, the
result numbers in the corresponding column will also be changed; If the item type/name is
canceled, the corresponding result numbers will also be decreased.
Fig. 5-16 HP verification

8. Click Next to view all HP pictures of the sample in full-view and repeat step 6 and 7 until
finished.
Save Result
9. Click Save button under the Current Cell Counting field to confirm the verification result of
selected sample, and the sample status is changed to V.
5.9.2 Advanced operations
After mastering the basic operations, the user can use advanced operations for
verification.
LP Verification
1. Same as Basic operations step 1-2.
2. Click Modify, then click the "+ " symbol on the target needed to cancel , to cancel the
target. When the target is cancelled, the "+ " symbol will disappear, and the result of current
frame will reduce.
3. Keep Modify is selected, then click the target name in the Current Cell Counting field,
52
then click the "+ " symbol on the target needed to modify, the target name will change to the
selected name, and the "+ " symbol will also change. The result of current frame will increase.
Repeat this operation to modify other targets in the same frame.
finished.
HP Verification
6. Click Modify, then click the "+ " symbol on the target needed to cancel , to cancel the
target. When the target is cancelled, the "+ " symbol will disappear, and the result of current
frame will reduce.
7. Keep Modify is selected, then click the target name in the Current Cell Counting field,
then click the "+ " symbol on the target needed to modify, the target name will change to the
selected name, and the "+ " symbol will also change. The result of current frame will increase.
Repeat this operation to modify other targets in the same frame.
finished.
Save Result
5.9.3 Batch operations
The user can use batch operations to modify a specific targets in one frame or all frames.
LP Verification
2. Click Modify, then choose Save Type in the Clear Mark drop-down menu, then the batch
mofiy window will appear. Choose Current/All in Picture to proceed current frame /all frames,
in Type choose the target name to be cleared, in Change to choose Cancel, then click Save.
All the "+" symbol on selected targets in current frame /all frames will disappear, and the result
in current frame /all frames will become 0. Repeat this operation to clear other targets.
in Type choose the target name to be modified, in Change to choose the target name, then
click Save. All the "+" symbol on selected targets in current frame /all frames will change, and
the result in current frame /all frames will change. Repeat this operation to modfiy other
targets.
53
Fig.5-17 LP Batch Modify

finished.
HP Verification
in Type choose the target name to be cleared, in Change to choose Cancel, then click Save.
All the "+" symbol on selected targets in current frame /all frames will disappear, and the result
in current frame /all frames will become 0. Repeat this operation to clear other targets.
in Type choose the target name to be modified, in Change to choose the target name, then
click Save. All the "+" symbol on selected targets in current frame /all frames will change, and
the result in current frame /all frames will change. Repeat this operation to modfiy other
targets.
Fig.5-18 HP Batch Modify

finished.
Save Result
54
5.10 Print Report

It is recommended previewing the print parameters or modifying before printing. For
adjustment please refer to Section 4.5 Print Mode Setting and Section 4.6 Print Setting.
Fig.5-19 Print on Verification interface
5.10.1 Single Print

1. Enter the Microscopy or Verification interface.
2. Select a microscopic record in the Urine Micro field.
3. Click the Preview button to confirm, click Print to print.
55
Fig.5-20 Print Preview interface
5.10.2 Batch Print

1. Enter the Microscopy or Verification interface, click the input box after Batch.
2. If the samples numbers are continuous, enter start and end ID. For example, to print from
No.2 to No.16, type 2-16.
3. If the samples ID are discontinuous, enter separate ID number respective ID. For example,
to print No.2, No.8, No.9 and No.18, type “2, 8, 9, 18,” ( always ends with “,”).
5.11 Power Off

After all the specimens have been tested, click the “Quit” icon in the shortcut keys area to
open the quit popup window. Checking “Quit and maintain” allows the instrument to wash the
pipelines with maintenance liquid automatically. Checking “Quit and auto turn off” allows the
instrument to shut down automatically the computer system after exit. Both ways can be
chosen randomly.
Quit
Fig.5-21 “Quit” icon
56
Fig.5-22 Quit popup window

After the system shuts down, turn off the green main switch on the left side of the
instrument.
57
AVE-772 Quality Control
6 QUALITY CONTROL
6.1 Quality Control

The Quality Control program is used to collect QC data and display QC reports and
statistics. These functions are used to obtain, verify and monitor proper system operation.
6.1.1 Control Material

To transport, store and use of the controls, please refer to the user’s manuals
respectively.
6.1.2 Control Frequency

Controls should be run at least once every 24 hours or as specified in the Laboratory’s
QC Procedure.
Focus should be performed at least once every 24 hours, preferably before running the
controls.
6.1.3 Handling QC Material

Hold the QC material bottle upside down repeatedly and shake it gently until the cells at
the bottom are completely dispersed and mixed well under macroscopic observation.
Then pour the QC material into a clean test tube (about 2ml per test), and use it for
testing. The material may be maintained in the fridge at 2-8°C with a tinfoil paper sealing the
bottle after using.
6.1.4 QC Parameters Input

The QC parameters should be input or set before running QC. Different parameters input
screen can be accessed according to different QC types. Clicking “QC” allows to access the
QC selection screen and select “Urine QC” and “Chemistry QC” respectively as shown in
Fig.6-1. Select any one of them to access the corresponding QC parameter input screen.
Fig.6-1 QC selection window

A. Accuracy QC
Select “Accuracy QC” to enter the “Accuracy QC” screen. Input “Lot ID”, “Level”, “Start
Date” and the following parameters:
 Lot ID (for example: 20110407, 6-10 digits )
 Mean；
 SD (Standard Deviation)；
 CV (Coefficient Variation).
Note：After completing parameter input in a row, click the “ Save” button, and then move on to
58
the next row for input.

Click “Delete” to delete the information selected. Click “Print” to preview the charts to be
printed. Click “Quit” to quit the screen.
Fig.6-2 Urine Quantity QC screen

B. Sensitivity QC
Select “Sensitivity QC”( Fig.6-1) to enter the “Sensitivity QC” screen shown in Fig.6-3.
Just input “Lot ID” and click Save on this screen. Click Delete to delete the information
selected. Click “Quit to quit the screen.
Fig.6-3 Urine Sensitivity QC screen

C. Chemistry QC
Select Chemistry QC in figure.6-1 to enter the screen as shown in below. According to the
QC material instruction, input corresponding batch number and item standard value, then
click “Save”.
59
Fig.6-4 Chemistry QC parameter input screen

6.1.5 Running QC
The QC can be run after the parameters being entered. Just like sample testing, QC is
run on the “Microscopy” screen.
Operations:
1 Place the QC material in tube rack and place the rack at ready area.
2 Choose “Urine Micro” in the “Micro” box; “QC” in the “Type” box; and “Micro QC” in the
“QC Type” box. The default sample ID starts from 2001.
CAUTION: The Micro ID should be same as Sample ID.

3 Choose Accuracy/Sensitivity in the “Name” box, then choose LOT number in the “LOT”
box; For Accuracy QC, check the corresponding concentration in the “Concentration”
box; For Sensitivity QC, only LOT is needed.
4 Click the “Insert” button and the system will run the QC automatically.
Fig. 6-5 Insert STAT QC screen
5 After QC testing is finished, the results will be displayed in Urine Special area, and
there will show QC normal/ QC warning / QC out of control messages in the status bar.
60
6 If QC out of control message appears, see 6.2 Remedies on Out-of-control for

further processing.
Explanation about QC status
 QC normal: The results located within ± 1SD will give a 'QC normal' status.
 Warning: The results located between ±2SD with ±1SD will give a 'warning' status.
 OOC (Out of control): The results located out of ±2SD will give an 'Out of Control' status.
Further steps is needed (see 6.2. Remedies on Out-of-control)
Fig. 6-6 QC result
6.2 Remedies on Out-of-control

NO. Possible Reasons Solutions
1 QC material is not mixed sufficiently Make sure the sample is well mixed, then
do the QC test again.
2 QC material expired or invalid Change the QC material and do the QC
test again.
3 The flow path is not smooth, which may Check tubings and needles if there is
cause inaccurate sampling or filling of the clogging. If the sampling needle is
chamber. blocked, penetrate each one with the
steel in the sparts parts supplied.
4 Inaccurate sampling volume. Perform pump calibration.
5 Pipeline leakage. Check for pipeline leakage, if there is a
leakage, replace the pipeline.
6 Targets in the picture are not clear and can Adjust the microscope focus and image
not be recognized. settings.
6.3 Reviewing QC Results

Select “Urine QC” and “Sensitivity QC” respectively and enter the corresponding screen.
6.3.1 Accuracy Review
Select “Accuracy QC” to enter the “Accuracy QC” screen shown in Fig.6-2. In the “Lot ID”
box, enter the ID or check the ID through the drop-down menu. After validation, corresponding
information will appear in the “Parameter” field on the screen, and QC Chart will also appear
in the Accuracy QC Chart.
The default statistic data is the last QC result of the day. You can also double click the QC
results of the day and choose one as the statistic data as needed.
61
Fig.6-7 Urine Quantity QC statistics
Fig.6-8 QC data selection
6.3.2 Sensitivity Review

Select “Sensitivity QC” to enter the “Sensitivity QC” screen shown in Fig.6-8.
In the “Lot ID” box, enter the ID or check the ID through the drop-down menu. After
validation, corresponding information will appear in the “Parameter” field on the screen.
Double-click the corresponding data on the page to pop up QC data selection prompt box
where the “mean” and “ID” can be set. Click “Save” for save. Click “Quit” to leave the page.
62
Fig.6-9 Urine Sensitivity QC data

The default statistic data is the last QC result of the day. You can also double click the QC
results of the day and choose one as the statistic data as needed.
6.3.3 Chemistry Review

Select Chemistry QC to enter the QC result list as shown in below.
Fig.6-10 Chemistry QC result

In the ” batch ID” of the figure, input or click drop-down menu to choose the QC batch
number that you want to consult result. After you have confirm it, corresponding QC result
value will appear in QC setting value information, and in below QC curve graph will appear
corresponding QC statistical chart.
1.Choose different batch ID can look though corresponding batch ID’s QC standard value
and corresponding QC data, and displays of already exited QC data’s corresponding diagram.
2.Choose different item can see different item’ S QC result curve and print it.
3.If there are more than two pieces of QC data one day, choose one of them as that day’s
QC data as you can see in figure 6-11.
63
Fig.6-11 Chemistry QC result

Notes: If the “item name” data displays red color, it indicates that the QC is out of control that
day. To say more specific, it indicates that corresponding date‘s QC data script is red, too.
Under the condition of many QC data appears some day, choose the last piece of data as a
benchmark if there isn’t display that a certain piece of data should be viewed as a benchmark.
Otherwise, treat the last piece of data as a benchmark. “deadline”: It means that the final QC
data of present QC batch ID.( It displays only when QC data has already exist).
“Print”: This botton can display the Chemistry QC statistics of present batch ID as it
shown in below:
Fig.6-12 Print preview of Chemistry QC statistics
64
AVE-772 Adjustment
7 ADJUSTMENT
7.1 Summary
The AVE-772 Analyzer’s adjustment include: Microscope Focus and CCD Image
Parameters adjustment, Pump Calibration and Blank Calibration etc. Enter the main screen
and choose “Management” to open the “Adjustment” submenu (shown in Fig.7-1).
Fig.7-1 Adjustment Screen
7.2 Microscope and Image Adjustment

The Microscope is an indispensable instrument in the medical examination. Its
view image clarity directly influences the microscopic results.
Since the AVE-772 is based on an automatic microscope as the testing platform, the
quality of the results mainly relies on the adjustment of the microscope in the AVE-772
adjustment procedure. The adjustment procedure consists of the focus adjustment and the
CCD parameters adjustment.
Make sure to have the instrument turned on at least for 30 minutes before
performing the focus adjustment in order to have stabilized the lamp of the
microscope!
While mark position and focus are not satisfy with the standard gallery, focus and
mark position calibration are recommended.
While brightness and RGB are not satisfy with the standard gallery, image
adjustment is recommended.
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AVE-772 Adjustment
7.2.1 Preparations for microscope focus adjustment

1. Prepare a suspension of RBCs (like a visual hematuria sample) with a standard tube, and
divide it into three tubes, then place the tubes in a rack. You may use the high level of the
Quality control for the microscope focus adjustment.
2. Locate the racks holding the tubes with the suspension of RBCs to the ready area of the
sampling module.
3. Select “CH1” then click “MechRS” button. There isn’t any fixed adjustment sequence for
the channels, however it is suggested to start from Channel 1, then Channel 2 and finally
Channel 3.
4. Click the “Samp All” button and the instrument will transfer the rack from the ready area to
the sampling area.
5. Wait for a about 2 minutes for the RBCs suspension to be fully aspirated into the Counting
Chamber, then the preparatory work is done and the next steps can be performed.
7.2.2 Adjust The Square Mark Position Of Microscope

The Microscope’s mark position, the position of the Counting Chamber square mark in the
microscopic view, determines the initial position of capturing images under different view fields
during microscopy. (The square mark is engraved at the bottom of each counting chamber).
Since the microscope has two view fields, a high power field (HP) and a low power field (LP),
the adjustment for the square mark position needs to be done for both.
The reference steps of each channel are in the following table:
Steps Focus
Left-right F-Backward Up-down Lens
Channel Lamp
LP 400～800 600～1000 700～1600 50～250 700
CH1 Difference Difference
HP Same as LP 700～850 200
with LP≤120 with LP≤120
Difference
LP 400～800 700～1600 50～250 700
with CH1≈2835
CH2
Difference Difference
Difference
LP 400～800 700～1600 50～250 700
with CH2≈2835
CH3
Difference Difference
Each Counting Chamber will provide these parameters, when the Counting Chamber is
replaced, click “Management” - “Adjustment”, then click Replace chamber, and in the popup
window, enter the new parameters, click Save , then click Quit.
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AVE-772 Adjustment
Fig.7-2 Replace chamber window

After the new parameters are saved, the instrument will automatically adjust, when it is
done, check the square mark and focus of each channel; If the square mark and focus are not
in the best condition, please adjust these parameters refer to the following steps.
1. Choose CH1, and click LP Reset;
2. Check the , and enter a certain step in “Step”(for example 50), click “-” or
“+” to adjust the steps to 550;
3. Check the , and enter a certain step in “Step”(for example 50), click “-” or “+”
to adjust the steps to 400;
4. Check the , and enter 50 steps in “Step”, click “+” to move the chamber until
the square mark appears;
5. Check the and , and enter 5-10 steps in “Step”, click “-” or “+”
to move the chamber until the square mark at the upper left in the view field, then click Save
LP Mark;
Fig. 7-3 LP mark

6. Click HP Reset, change to HP, then check the and , and
enter 5-10 steps in “Step”, click “-” or “+” to move the chamber until the square mark at the
upper left in the view field, then click Save HP Mark;
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AVE-772 Adjustment
Fig. 7-4 HP mark
7. Follow step 1-6 to adjust CH2 and CH3.
Note: The F-Backward step of CH2 & CH3 is 3385 & 6220.
7.2.3 Focus Adjustment

When the adjustment of mark position is done, focus adjustment is needed.
Procedure of Focus Adjustment
1. Check the Up-down value of CH1 and just remember this value. Then tap HP Reset
button to switch to HP lens.
Fig. 7-5 LP interface

2. In the HP lens interface, check whether the Up-down value is same with that of LP lens. If
the two values are not equal, please go to Step3. If the two values are equal, please just
68
AVE-772 Adjustment
go to Step4.
Fig. 7-6 HP interface

3. Make Up-down option tapped, and input proper value in the text box which located behind
Step:. Then tap + or – button to adjust the Up-down value same with that of LP lens. Tap
Save HP Mark button to save the change.
Fig. 7-7 HP adjustment
69
AVE-772 Adjustment
4. Switch to CH2 and CH3 respectively, and repeat step 1-3 to finalize the settings.
5. Reture to CH1 and tap LP Reset button to switch to LP lens, and click Auto Focus and
then the microscope will perform focus calibrating automatically.
Fig. 7-8 Auto focus

6. A pop-up window will show a calibrated parameter after auto focusing. Click Save to save
the calibration.
70
AVE-772 Adjustment
Fig. 7-9 Auto focus confirm

In general, the three channels lie nearly in the same plane, which means that the
focus of the other two channels will be adjusted according to the alteration of
channel 1. Just to be sure, it’s recommended to check the focus of the two
channels, and if it’s necessary, the user need to adjust the focus manually by
following up the next steps.
7. Select “CH2” in the “Channel” field on the upper-left of the adjustment screen, then the
lens will switch to channel 2.
8. Check the Up-down value of CH2 and just remember the value. Then tap HP Reset
button to switch to HP lens.
Fig. 7-10 LP of channel 2

9. In the HP lens interface, and input proper value in the text box. Then tap + or – button to
adjust the focus to a sharp condition. Remember the Up-down value and DO NOT SAVE
THIS VALUE for the moment. Tap LP Reset button to switch to LP lens interface.
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AVE-772 Adjustment
Fig. 7-11 HP of channel 2

10. In the LP lens interface, make Up-down option tapped, and input proper value in the text
box which located behind Step:. Then tap + or – button to adjust the Up-down value
same with that has the best focus of HP lens. Tap Save LP Mark button to save the
change.
Fig. 7-12 LP adjustment
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AVE-772 Adjustment
11. Switch to CH3 and repeat step 8-10 to adjust the focus of CH3.
7.2.4 Image Adjustment

Click “Image Setting” to enter the “Image Adjust” window (Fig.7-13). The CCD
Background Exposure and White Balance Values can be adjusted on the screen as well as
the settings for the standard gallery.
Fig.7-13 Image Setting Screen

The reference for the parameters are:
Gray scale:12;
Gain: 15-25;
Red/Green/Blue: The difference between Picture value and Reference value should less
than 10.
To adjust the image settings, refer to the following steps:
1. Check “HP” in the “Precise Adjust” field to enter the HP field.
2. Click “AE On” to wait for automatic exposure.
3. Input the fine-tune value and gain value in the “Bright/Gray scale” in the “Precise
Adjust” field according to the image and the reference value.
4. Click the “L+” or “L-” button in the “Brightness” field to make the exposure value of the
image consistent with the reference value (values of +/- 5 are considered as
consistent). Click “Save Exposure” for saving the settings.
5. Click “AWB On” to wait for automatic white balance adjustment. Click the “R+/R-”,
“G+/G-”, and “B+/B-” button respectively to allow the image value to be as close as
possible to the reference values (values of +/- 10 are considered as consistent). Click
“Save Gain”.
6. When the exposure values and white balance values under HP are adjusted, click
“Save All”.
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AVE-772 Adjustment
7. Check “LP” in the “Precise Adjust” field to enter the LP filed; the operations are the
same as HP. Click “Save All” to save all the adjusted parameters.
7.3 Pump Calibration

The pump calibration should only be modified by authorized technicians!
Pump calibration is recommended at following conditions:
1. Bubbles occur in counting chamber or the amount of reagent is abnormal;
2. After replacement of Rinse/Waste Bath or tubes (including valve tube, pump tube
etc.);
3. Monthly maintenance.
7.3.1 Operations
The pump calibration can be performed at the lower right of the adjustment screen.
Fig.7-14 Pump Calibration on the Adjustment screen

“Wash-pump Calib”： By selecting “WPC” (1#, 2# and 3# respectively), a dialog box will
open (shown in Fig.7-15). Click “OK” to continue, the new value will be saved automatically.
The reference value for Wash-pump Calib is 3.5-5s, repeat wash pump calibration, till the
value reaches the reference range.
Fig.7-15 Start box for “Washing-pump Calib”

“Sample-pump Calib”：By selecting “SPC” (1#, 2# and 3# respectively), a dialog box will
open (shown in Fig.7-16). Click “OK” to continue, the new value will be saved automatically.
The reference value for Sample-pump Calib is 4-5.5s, repeat sample pump calibration, till the
value reaches the reference range.
Fig.7-16 Start box for “Sample-pump Calib”
7.3.2 Caution
If the wash/sample calibration value is abnormal in the process of Pump Calibration,
please check whether the pipeline is clogged.
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AVE-772 Adjustment
7.4 Blank Calibration

The operation is mainly used for determing dirty spots number in the Counting Chamber.
Operations can be done on the right side of the “Calibration” screen, in the “Blank Calib”
field.
1. Click “Blank” button. The instrument will run the Blank Calibration automatically.
2. A prompt will appear at the end of the blank calibration.
Fig.7-17 Blank Calibration window

3. Click “OK” to save dirty spots number, Click “Cancel” not to save. Please note that
those will be susbtracted from each result randomly. Therefore we recommend to use the
blank calibration to determine, if the the channels are clean.
CAUTION:If the dirty spot exceeds 5, please perform special wash, then perform
Blank Calibration again; If the dirty spot still exceeds 5, it is recommended to replace the
counting chamber.
75
AVE-772 Results
8 RESULTS
8.1 Urine Data
8.1.1 QC Inquiry
Click “Data”-“Urine Data”-“QC Inquiry” to enter the QC Inquiry Screen.
The user can inquire and backup previous QC records.
Fig.8-1 QC Inquiry Screen

Inquiry：Inquire QC records.
Backup：Backup selected records to Excel file.
Quit：Quit the inquiry screen.
8.1.2 Urine Inquiry
History urine records can be inquired with the “Urine Inquiry” program. The user can
inquire, delete, and print previous records which include patient’s information, microscopic
results and chemistry results.
1. Enter the main screen;
2. Click “Data”-“Urine Data”-“Inquiry”, or click the “Urine Inquiry” icon in the shortcut
keys area.
Fig.8-2 Urine Inquiry shortcut
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AVE-772 Results
The user can inquiry, backup, preview and print history records (Shown in Fig.8-3).
Fig.8-3 Urine Inquiry Screen

Inquiry：Inquire history data.
Preview：Preview a selected sample’s report.
Backup：Backup selected result to Excel file.
Quit：Quit the inquiry screen.
Inquire records
a. By patient’s information
1. Input patient’s various inquiry items in the “Inquiry Condition” field, click” Inquiry”
button at the lower right of the field. System will inquire automatically according to what you
have set and display the results in the “Inquiry Result” field.
2. Input one or more inquiry items as shown in Fig.8-3, click “ Inquiry” button. The
inquired results will appear in the “Inquiry Result” field.
b. By sample ID
Input the date as YYYY/MM/DD into the textbox of “Sample ID” to display the patient’s
information of the day in the “Inquiry Result” field. For example, input “200606060006” to
display the records of sample No.6 on 2006-06-06.
c. Inquire all
Select a time range, click the“ Inquiry” button. All the records of the patient will appear in
the “Inquiry Result” field.
Print records
Click Preview button to preview a selected sample’s report, in the perview window, click
Print to print the report.
8.1.3 Statistics
Enter the main screen, select “Data” - “Urine Data” -“Statistics” to enter the urine
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AVE-772 Results
statistics screen. Workload, urine result and chemistry result can be counted here. The type of
the statistics varies with the static option. Both test results of the day and those without
patient information cannot be counted.
Fig.8-4 Statistics Screen

From/To: Statistics by time period.
Dept: Statistics by the clinical lab department.
Tested: Statistics by operator.
Subm. (Submitter): Statistics by submitter.
Statistics: Perform the statistics.
8.1.4 History Data Verification
Enter the main screen, select “Data”-“Urine Data”- “Data Verification” to enter the
history urine verification screen as shown in Fig.8-5.
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AVE-772 Results
Fig. 8-5 History Urine Verification Screen

Select the microscopy time, then you can perform the verification, mofify patient
information and print report.
Every time the operator makes verification, the instrument will automatically record the
result, and generates a history record; The operator can review all the informations of each
sample, including verified date, time and verifier in Audit Trail area.
8.2 Tube Records

Tube Records is mainly used to determine whether the actual sample tube number is
consistent with the automatic one identified by the system. Click “Data”-“Tube
Records”-“UrineTube Records” to review today’s finished sample tube and rack records.
Move the cursor on the tube icon, it will show the sample ID, barcode, rack number and
tube number for further verificaiton.
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AVE-772 Results
Fig.8-6 Tube Records
8.3 Positive List

Click “Tools”-“Positive List” in the “Microscopy” interface to review today’s positive
sample list.
The Microscopy Positive list displays those samples where any data of RBC, WBC, Cast,
Crystal and Epithelium is positive. The Chemistry Positive list displays those sample where any
result of BLD, LEU and PRO is positive.
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AVE-772 Results
Fig.8-7 Positive List

Click Print button to print the current positive list.
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AVE-772 Maintenance and Service
9 MAINTENANCE AND SERVICE
9.1 Maintenance
9.1.1 Precautions
WARNING: Wear protective gloves to prevent exposure to pathogens. Discard

contaminated materials according to applicable regulations.
9.1.2 Daily Maintenance
Items Suggested Intervals
System maintenance (including QC) Daily
Cleaning instrument surfaces Daily
Cleaning Counting Chamber surfaces Daily
Cleaning Waste Tank Daily
9.1.3 Periodic Maintenance
Cleaning the Automatic Sampler Weekly
Checking microscope focus (Focus Adjustment) Weekly
Cleaning the Strip Conveyor Weekly
Cleaning the Pipette Slot Weekly
Checking pinch valves and moving pinch location in case of Monthly
adhesion
Cleaning the Waste Pool Monthly
Pump Adjustment Monthly
Cleaning Sampling Needle Monthly
Cleaning Photoelectric Sensors on Auto-sampler Monthly
Checking microscopic image background (digital CCD
Semi-Annually
background exposure and white balance values adjustment)
Counting Chamber “Blank Calibration” Semi-Annually
Replacing the Pump tubes Semi-Annually
Checking microscope mark position As needed
Replacing the microscope lamp As needed
Maintaining and Replacing the Counting Chamber As needed
Dredging Sampling needle As needed
System Back up According to Laboratory
Protocol
Results Back up According to Laboratory
Protocol
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9.1.4 Consumable Replenishment

Replacing the reagent pack for AVE-772 As needed
9.2 Daily Maintenance

This procedure should be performed at least once daily.
9.2.1 Cleaning Instrument Surfaces
Tools required: Disinfectant/cleanser, gauze, and protective gloves.
Procedure: Power off the instrument. Clean the instrument using gauze moistened in
disinfectant/cleanser. Wipe again using distilled water and then make it dry.
9.2.2 QC
Refer to 6 QUALITY CONTROL for details.
9.2.3 Cleaning Counting Chamber Surfaces
Tools required: 95% absolute alcohol, absorbent cotton swabs.
Procedure: Open the front cover, using a clean cotton swab moistened with 95% absolute
alcohol; wipe the Counting Chamber surfaces gently.
CAUTION: Do not wipe back and forth, wipe in one direction to avoid scratches on the
counting chamber surface!
9.2.4 Cleaning Waste Tank

Tools required: Disinfectant (such as solid dialdehyde, peroxide acetic acid, hydrogen
peroxide, chlorine dioxide, etc.), distill water, gauze, protective gloves.
Procedure: Take out the waste tank from the analyzer, and clean it with the disinfectant.
Then use distill water to clean it again and let it dry and put it back.
9.3 Weekly Maintenance

9.3.1 Cleaning the Sampler
To make sure the sampler running properly, timely cleaning the sampler is highly
recommended when there are liquid leaked on the sampler.
CAUTION: Immediately power off the sampler and clean it while a whole rack samples
are pouring onto the sampler.
Tools required: Disinfectant/detergent, gauze, and protective gloves.
Procedure:
1 Power off the instrument. Ensure that the maintenance is performed under power cut-off
condition.
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2 Moisten gauze with disinfectant/cleanser and wipe the sampler to remove any deposits.
3 Check the flexibility of pawls. Moisten cotton swabs with disinfectant/cleanser to clean
pawls, remove any deposits.
4 Wipe again using distilled water.
5 Power on the instrument until the sampler is totally dry.
9.3.2 Checking microscope focus (Focus Adjustment)

Refer to 7.2.3 Focus Adjustment for details.
9.3.3 Cleaning the Strip Conveyor

Tools required: Disinfectant/detergent, cotton swabs, brush and protective gloves.
Procedure:
condition.
2 Remove the right panel of the instrument, and take out the Strip Conveyor’s Plate.
3 Use brush with disinfectant/cleanser and clean the plate to remove any deposits.
4 Clean it again using distilled water and let it dry.
5 Put the plate back and close the right panel of the instrument.
9.3.4 Cleaning the Pipette Slot

Tools required: Disinfectant/detergent, cotton swabs, brush and protective gloves.
Procedure:
condition.
2 Open the front panel of the instrument.
3 Use brush with disinfectant/cleanser and clean the Pipette Slot to remove any deposits.
4 Clean it again using distilled water.
5 Close the front panel of the instrument.
9.4 Monthly Maintenance

9.4.1 Checking pinch valves and moving pinch location in case of adhesion
Since the pinch pipelines are in the clamped state while the valves are not energized, monthly
perform rebound maintenance to prevent adhesion is recommended.
Procedure1: Take out the pinch from the valve and twist it gently by fingers to retrieve its’
elasticity.
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Procedure2: Move the pinch pipelines upper or down 2mm per month to prevent adhesion or
broken after long time clamped.
Contact the service for replacement if you need a new pinch pipeline.
9.4.2 Cleaning the Waste Pool
In order to prevent pipeline block and invalidation of liquid detection, the waste pool is suggested
to perform cleaning every month. This includes cleaning the inner wall, cleanser nozzle upper and
waste nozzle at bottom.
Tools required: Deionized water, cotton swabs and protective gloves.
Procedure:
1 Make sure the analyzer is in shutdown state.
2 Open the front cover to access the waste pool. Manually lift the sampling needle out.
3 Using a tube brush moistened with deionized water, remove salt deposits present on the
waste pool. Clean the inner wall.
4 Using a cotton swab moistened with deionized water to swab cleanser nozzle upper and
waste nozzle at bottom.
5 Move the sampling needle back into the waste pool, close the front cover.
6 Power on the instrument and login the software. Click Wash All 1-2 times in Adjustment
interface to wash all the channels.
CAUTION: Do not insert the cotton swab inside the waster pool; cotton particles may
clog the tubing connectors.
9.4.3 Pump Calibration
Refer to 7.3 Pump Calibration for details.
9.4.4 Cleaning Sampling needle
Tools required: 95% absolute alcohol, cotton swabs and protective gloves
Procedure:
3 Using a cotton swab moistened with absolute alcohol to swab the interface of needle,
4 Move the sampling needle back into the waste pool, close the front cover.
9.4.5 Cleaning Photoelectric Sensors on auto-sampler
Cleaning photoelectric sensors on auto-sampler is recommended to perform every month.
Tools required: 95% absolute alcohol, cotton swabs and protective gloves
Procedure:
1 Power off the instrument, make sure the operation is in shutdown state.
2 Using a cotton swab moistened with absolute alcohol to swab the surface of photoelectric
sensors
3 Swabs dry them by using a dry cotton swab.
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9.5 Semi-Annual Maintenance

9.5.1 Check Microscopic Image
Check the background of CCD images by comparing it with the standard images. If there is any
deviation, refer to 7.2.4 Image Adjustment for further adjustment.
9.5.2 Blank Calibration
If some spots impossible to clean out are found in the counting chamber, please refer to 7.4 Blank
Calibration for blank calibration.
9.5.3 Pump tube replacing
Since the pump tube is a kind of wearing parts, it should be checked regularly. Normally pump
tube should be replaced every 6 months. If any aging, deformation, inelasticity or broken is
happen, please replace it immediately.
The specification of pump tube is Φ3.2*Φ6.4*92mm, make sure using the same pump tube for
replacement.
WARNING: Wear the personal protective equipment such as protective clothing
and glove to protect you from the waste remaining in the pump tube!
Fig.9-1 Side view of pumps

Replacement procedure:
1 Open the pump cover by anticlockwise revolving.
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Fig.9-2 Open the pump cover
Fig.9-3 Side view of the opened pump

2 Take both ends of the pump tube off from the groove then pull the entire pump tube out
from the pump.
Fig.9-4 Remove the pump fittings
87
Fig.9-5 Remove the pump tube

3 Disconnect the two ends of tube; replace the new pump tube by connecting both ends to
the connectors;
CAUTION: Make sure stretching the new pump tube before replacement.
Fig.9-6 Replace the new pump tube

4 Push the new pump tube back into the pump and well fix the two ends to the groove.
CAUTION: After installing the new pump tube into the pump, make sure the rotor can
spin smoothly, , the pump tube should be no distortion, not protrude from the pump.
88
Fig.9-7 Install the pump tube back to the pump

5 Install the cover back.
Fig.9-8 Install the cover
CAUTION: In order to prevent affecting the pump accuracy, refer to 7.3 Pump
Calibration to perform the pump calibration after replacing the pump tube!
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9.6 As Needed Maintenance

9.6.1 Checking the Mark Position of Microscope
Refer to 7.2.2 Adjust The Square Mark Position Of Microscope for details.
9.6.2 Replacing the microscope lamp
Since the microscope lamp is a kind of wearing parts, its life may affect by environment (voltage,
temperature, humidity). It should be replaced according to the actual working situation.
If its lightness become poor or the background of images becomes dark, thus the recognition is
affected by this, please replace it immediately.
Specification of Microscope lamp: 2V，20W
CAUTION: Replace the new lamp of same specification!
CAUTION: Make sure the replacement is done in power off state! To prevent burning
by the lampshade, make sure it is already cooled before replacement!
1 Power off the instrument; make sure the lampshade is already cooled. Open the front cover to
find the fix screw located on the base.
Fig. 9-9 Fixed screw

2 Loosen the fix screw to open the lampshade;
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Lamp shelter
Fig. 9-10 remove the shelter

3 Remove the shelter;
Lamp
Fig. 9-11 Replace the lamp

4 Unplug the old lamp and re-install the new one. Make sure it’s plugged reliable.
CAUTION: Socket oxidation may cause contact problem. If the lamp still does not
light on, please repeat plug several times.
91
9.6.3 Replacing the Counting Chamber

Maintaining the Counting chamber
The counting chamber is a kind of wearing parts, its life may affect by the frequency of use and
maintenance condition. If there are spots inside it or it was blocked, following the next procedure.
1 Login the software and go to Adjustment interface, select the channel need to be cleaned,
click Wash+ to see whether it can be cleaned, if not, please refer to step2.
2 Remove the counting chamber. Connect a injector to one end via chamber pipeline, soak
another end into a container filled with cleanser (diluted hydrochloric acid, disinfectant,
detergent solution, for example). Repeatedly perform aspiration until the dirty are discharged.
3 If above steps do not work, please replace the counting chamber.
Replacing the Counting Chamber
When the counting chamber is broken or there are too many spots or scratch on it, this may affect
the recognition, please replace the counting chamber. Make sure the replacement is done in
power off state.
1 Compare the serial number printed on the side of counting chamber with that on the insert
card to check if they are matched. If so, remove the hose connected with the two ends.
2 Open the front panel of instrument.
Fig.9-12 Counting chamber with bracket

3 Manually switch the LP lens aside, lift the two tabletting up to pull out the bracket together with
counting chamber; take off the chamber pipeline connected at both sides, then take out the
bracket entirely.
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Fig.9-13 Pull out the counting chamber bracket

4 Loosen the Counting Chamber fixed screws to take out the Counting Chamber;
Fig.9-14 Release the counting chamber from the bracket

5 Replace the new counting chamber. Connect the chamber pipelines onto both sides of three
channels respectively, the channels can be identified by its color ring.
CAUTION: The installation trends should follow this: the face with eminence is upper
surface and the other one is on the bottom; the side with serial number printed on should
be toward inside. The chamber pipeline should be plugged to 1/2 of iron tube (as shown
below).
93
Fig.9-15 Replace with a new counting chamber

6 Install the new counting chamber into bracket; press tightly the screws to fix it.
CAUTION: The counting chamber should be installed as much as possible into

upper right corner of bracket. The chamber tabletting should press to the chamber edge
from 1-2 mm.
Fig.9-16 Fix the counting chamber to the bracket

7 Push the bracket back under the tabletting. Make sure the positioning balls just drop into the
fixed holes.
8 Close the front panel.
9 Input the parameters of new counting chamber.
After replacing the counting chamber, the parameters should be input in the software. In the main
94
interface, go to Management-Adjustment, click Replace Chamber button to pop-out the

Replace Chamber interface, in which input the parameters of new counting chamber. The user can
find the related parameters on the insert card. Click Save button to make the inputting take effect.
Click Quit button to finish the inputting.
Fig. 9-17 Replace Chamber interface

10 Focus confirmation
After inputting the parameters, the microscope will respond the inputting to find its location
automatically. After that, it’s necessary to check focus and mark under LP & HP of three channels.
If the condition is not satisfied, refer to 7.2 Microscope and Image Adjustment for further calibration.
Normally the user just needs to perform fine turn to get better result.
9.6.4 Dredging Sampling needle
If the sampling needle is blocked, following the below steps to dredge the sampling needle.
3 Remove the pipelines from the top of needle, use the pricker thread through from top of the
needle and exit out from the end, clean the dirty stick on the pricker;
4 Repeat step3 several times until there is no dirty stick on.
5 Connect the pipeline back to the needle. Move the needle back to the waste pool and close
the front panel.
6 Restart the instrument and login the software, go to Adjustment interface and click Wash All
button several times to perform channels washing.
9.7 Data Backup/Restore

The instrument may run slower after a certain period of time due to the accumulation of
patient data. To make data backup periodically is recommended.
Go to Management-Backup to entry Backup interface as shown below.
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Fig.9-18 Start Backup

9.7.1Data Backup
Auto Mode
To enable auto backup the examination results and system parameter to the specified path
monthly, you just need to select Monthly automatic.
Manual Mode
The examination results and system parameter can also be export manually. Follow the steps
below.
1 Click Browse button to choose the specified backup path.
2 Select tick box in Urine Data or Parameter area to backup data need to export.
3 Click Export button to export the selected item to the specified path.
4 Click Backup button at Backup Setting area to make a whole backup of the database.
Meanwhile a record will be recorded in Restore Setting list.
CAUTION: To prevent losing data, do not perform any other operation during the
process of backup.
9.7.2 Data Restore
CAUTION: Only properly trained and authorized service personnel can operate
restoring. Make sure that your operation are under the instruction of authorized service!
Operation procedure:
1 Click Browse button to choose the specified restore path.
2 Click File button to select files (bak. format) need to be restored in specified path.
3 Click Open button to start restoring your selected data.
4 Select tick box in Urine Data or Parameter area to restore data need to import.
5 Click Import button to import the selected item from the specified path.
96
CAUTION: To prevent losing data, do not perform any other operation during the
process of restore.
9.8 Troubleshooting
9.8.1 Shutting Down the Instrument
When it is necessary to restart the instrument, shut down the instrument and the
computers in the following order:
1 Click Quit button on the Microscopy screen.
2 Select Quit and auto turn off, unselect Quit and maintain, then click Quit button.
3 After the device shutting down, turn off the power of sampler.
4 Turn off the switch at the left of instrument.
9.8.2 Restarting the Instrument
1 Turn on the switch of the instrument.
2 Turn on the power of sampler.
3 Press on the power switch on the front panel to start the system.
4 Double-click the software icon on the desktop. Login the system.
9.8.3 Error Messages
NO. Error description Error elimination

Login the system, the system notices 1 Re-plug or replace the watch dog.
as ‘Illegal user’ 2 Shut down the instrument and then restart.
1 3 If the problem cannot be eliminated, please
contact the authorized service personnel.
After starting the examination, the 1 Check whether the switch of sampler is turn
system notice as ‘Sampler on. If not, turn on the switch;
communication timeout’. 2 Confirm the error message, click Forced
button to forced stop the examination;
2
3 After the examination is stopped, click Start
button for recheck.
4 If the problem cannot be eliminated, please
The system notice as ‘Push-in motor 1 Check whether the sampler is placed at a
at ready area reaching position direct strong light area. If so, prevent the
error.’ sampler from strong light.
3 2 Confirm the error message for its own
eliminated.
3 If the problem still remains, shut down the
instrument and then restart.
97

During examination, the tube rack 1、 Click Forced button to forced stop the
cannot be shifted by the pawls at examination. Take the tube rack out from
testing area. testing area.
2、 Check whether the tube rack is for specialized
use. Change the tube rack if it is not.
4 3、 Check the flexibility of pawls. Moisten cotton
swabs with disinfectant/cleanser to clean
pawls, remove any deposits.
4、 Restart the instrument for further check. If the
problem cannot be eliminated, please
Sample was missed examination by 1 Shut down the instrument and then restart.
5 instrument. 2 If the problem cannot be eliminated, please
Free position on tube rack was exam 1 Check whether the sampler is placed at a
by accident. direct strong light area. If so, prevent the
sampler from strong light.
6
2 Shut down the instrument and then restart.
The system notice as ‘Push-out 1 Check whether the sampler is placed at a
motor at tested area reaching direct strong light area. If so, prevent the
position error.’ sampler from strong light.
7
The system notice as ‘Tested area is 1 Remove all the finished tube racks at tested
full.’ area and confirm the error message for its
own eliminated.
2 If the problem still remains, check whether the
sampler is placed at a direct strong light area.
8 If so, prevent the sampler from strong light.
Confirm the error message for its own
eliminated.
98

The system notice as ‘Loading motor 1 Click Forced button to forced stop the
at bridge area home position error.’ examination. Shut down the instrument and
9 then restart.
The system notice as ‘Push-in motor 1 Click Forced button to forced stop the
at ready area home position error.’ examination. Shut down the instrument and
10 then restart.
The system notice as 1 Click Forced button to forced stop the
‘Sample-switching motor at testing examination. Shut down the instrument and
11 area home position error.’ then restart.
The system notice as ‘Push-out 1 Click Forced button to forced stop the
motor at tested area home position examination. Shut down the instrument and
12 error.’ then restart.
The system notice as ‘Loading motor 1 Click Forced button to forced stop the
at bridge area mechanical error.’ examination. Shut down the instrument and
13 then restart.
The system notice as ‘Push-in motor 1 Click Forced button to forced stop the
at ready area mechanical error.’ examination. Shut down the instrument and
14 then restart.
The system notice as 1 Click Forced button to forced stop the
‘Sample-switching motor at testing examination. Shut down the instrument and
15 area mechanical error.’ then restart.
The system notice as ‘Push-out 1 Click Forced button to forced stop the
motor at tested area mechanical examination. Shut down the instrument and
16 error.’ then restart.
99

The system notice as ‘Sampling 1 Confirm the error message for its own
channel leakage or blockage.’ eliminated.
2 If the problem still remains, check whether
the sampling needle was blocked. If so, refer
to 9.6.4 Dredging Sampling needle to dredge
Sampling needle.
3 Open the left cover of instrument; refer
to 9.4.1 Checking pinch valves and moving
pinch location in case of adhesionto perform
17 pinch pipelines maintenance. Find the pump
and open the its cover to check whether the
pump tubes were broken. If so, please refer
to 9.5.3 Pump tube replacing to replace the
pump tubes.
4 Check whether the counting chamber was
blocked. If so, refer to 9.6.3 Replacing the
Counting Chamber for further process.
The system notice as ‘No cleanser 1 Confirm the error message for its own
A’. eliminated.
2 Check whether cleanser A was run out. If so,
replace a new one.
3 If the problem still remains, open the left
cover of instrument; refer to 9.4.1 Checking
pinch valves and moving pinch location in
18 case of adhesion to perform pinch pipelines
maintenance.
4 Find the pump and open the its cover to
check whether the pump tubes were broken.
If so, please refer to 9.5.3 Pump tube
replacing to replace the pump tubes.
The system notice as ‘No cleanser 1 Confirm the error message for its own
B’. eliminated.
2 Check whether cleanser B was run out. If so,
replace a new one.
19
case of adhesion to perform pinch pipelines
100

maintenance.
The system notice as ‘No 1 Confirm the error message for its own
maintenance cleanser’. eliminated.
2 Check whether maintenance cleanser was
run out. If so, replace a new one.
20 case of adhesion to perform pinch pipelines
maintenance.
The system notice as ‘Waste 1 Confirm the error message for its own
container is full’. eliminated.
2 Check whether the waste container is full.
21 Discard waste according to applicable
regulations.
The system notice as ‘Cleaning pool 1 Click Forced button to forced stop the
overflow’. examination. Shut down the instrument.
2 Open the front panel of instrument. Check
whether there is liquid overflow from the
waster pool. If so, please wipe clean.
3 Refer to 9.4.2 Cleaning the Waste Pool to
22 perform waste pool maintenance.
4 Restart the instrument to check whether the
problem is eliminated.
5 If the problem still remains, open the left cover
of instrument; refer to 9.4.1 Checking pinch
valves and moving pinch location in case of
adhesion to perform pinch pipelines
101

maintenance.
6 Find the pump and open the its cover to check
whether the pump tubes were broken. If so,
please refer to 9.5.3 Pump tube replacing to
replace the pump tubes.
The system notice as ‘Cleaning pool 1 Confirm the error message for its own
drain pipe leakage or blockage’. eliminated.
2 If the problem still remains, click Forced
button to forced stop the examination. Shut
down the instrument.
3 Open the front panel of instrument, Refer
to 9.4.2 Cleaning the Waste Pool to perform
waste pool maintenance.
4 Restart the instrument to check whether the
problem is eliminated.
23 5 If the problem still remains, open the left
case of adhesion to perform pinch pipelines
maintenance.
The system notice as ‘Lift motor 1 Restart the instrument to check whether the
can't find zero point’ problem is eliminated.
24
The system notice as ‘Level motor 1 Restart the instrument to check whether the
can't find zero point’. problem is eliminated.
25
The system notice as ‘Sampling 1 If the sampling needle collision happened,
needle collision’. the instrument will continue to finish the
samples already under examination. When
26
all the examination is finished, a jump-up
window will be shown on the screen.
2 Check where and why the collision
102

happened. Manually remove the obstacles. If
it caused by using the unspecialized tubes,
please replace them.
3 After removing the obstacles, click OK
button. Click Start button to restart the
examination. Then remained rack will be shift
out. Re-exam the samples not examined.
The system notice as ‘No sample’. 1 The sample is too small to exam. Please
re-prepare sample according the requirement
and re-exam it.
27 2 Confirm the error message for its own
eliminated.
The background of images became 1 Shut down the instrument and clean the
dark. stage, lampshade of microscope and
surface of counting chamber.
2 The image needs to adjust. Refer to 7.2.4
Image Adjustment for image adjustment.
28
3 The microscope lamp is aging, please refer
to 9.6.2 Replacing the microscope lamp to
replace the lamp.
The focus is unstable. The cells in the 1 Check whether the LP&HP lens were loosen,
images are too dark or too bright, hard screw them if so.
to identify. 2 Make sure the instrument is warmed-up for
30 minutes after power on. Refer to 7.2.3
29
Focus Adjustment to adjust the focus and
re-exam.
The system notice as 1 Restart the instrument to check whether the
‘Forward-backword motor out of problem is eliminated.
30
range.’ 2 If the problem cannot be eliminated, please
The system notice as ‘Left-right 1 Restart the instrument to check whether the
motor out of range;’ problem is eliminated.
31
103

The system notice as ‘Up-down 1 Restart the instrument to check whether the
motor out of range;’ problem is eliminated.
32
The system notice as ‘Lens motor 1 Restart the instrument to check whether the
out of range;’ problem is eliminated.
33
The system notice as ‘Microscope 1 Restart the instrument to check whether the
communication timeout’ problem is eliminated.
34
The system notice as ‘Pump System 1 Restart the instrument to check whether the
communication timeout’. problem is eliminated.
35
The system notice as ‘Failed to save 1 Restart the instrument to check whether the
exposure!’ or ‘Open CCD error!’ problem is eliminated.
36
Turn on the power switch of 1 Maybe the power cable was not well
instrument, the instrument is connected. Re-plug the power cable;
unresponsive 2 The fuse is probably burned out. Replace a
new one with the same specifications.
CAUTION: Beware of electric shock!

37
Plug the power cable before operation!
CAUTION: The operation can only be

done by professionals.
The system notice as ‘Open DB 1 The database did not start properly.
error’. Double-click the database icon at the
bottom-right corner, then click start button to
38
reboot the database.
The system notice as ‘Initialization 1 Restart the instrument to check whether the
Error!’ problem is eliminated.
39
104

Login the software, anyone of below 1 Restart the instrument to check whether the
messages is noticed. problem is eliminated.
Failed to open LIS port! 2 If the problem cannot be eliminated, please
Failed to open chemistry port! contact the authorized service personnel.
40 Failed to open microscope port!
Failed to open pump port!
Failed to open sampler port!
Failed to open reagent port!
Failed to open barcode port!
Login the software, the system notice 1 Restart the instrument to check whether the
as ‘No recognition program!’ problem is eliminated.
41
The system notice as ‘LIS 1 Restart the instrument to check whether the
communication failure’ problem is eliminated.
42
Login the software, anyone of below 1 Re-plug or replace reagent sensor (together
messages is noticed. with reagent pack)
Invalid Serial Number! 2 Restart the instrument to check whether the
43
Invalid Password! problem is eliminated.
Authorization validation failed! 3 If the problem cannot be eliminated, please
The system notice as ‘Initialization 1 Restart the instrument to check whether the
Error!’ problem is eliminated.
44
The system notice as ‘Export results 1 Restart the instrument to check whether the
to LIS error!’ problem is eliminated.
45
The system notice as ‘The Refer to 6.2 Remedies on Out-of-control for
instrument is out of control during process
46
QC test, adjust the instrument and
retry!’
The system notice as ‘No response 1 Restart the instrument to check whether the
from lower computer, restart the problem is eliminated.
47
program after troubleshooting!’ 2 If the problem cannot be eliminated, please
The system notice as ‘Abnormal 1 Restart the instrument to check whether the
48 Data:’ problem is eliminated.
105

The system notice as ‘Failed to read 1 Restart the instrument to check whether the
or write registry!’ problem is eliminated.
49
To calibrate the pump system, the 1 Restart the instrument to check whether the
system notice as ‘Valve Test failed!’ problem is eliminated.
50
To calibrate the pump system in 1 Re-calibrate the pump system to eliminate
Adjustment interface, the system the error automatically.
notice as ‘Reagent Stop;’ 2 If the problem still remains, check if the
cleanser A was expired or with precipitate
inside. Replace a new one if it was expired.
51 Shake it well if there is precipitate inside.
3 If the problem still remains, refer to 9.4.2
Cleaning the Waste Pool to maintain the
waste pool.
The system notice as ‘The pump 1 Re-calibrate the pump system to eliminate
calib time is longer than expected’. the error automatically.
52
The system notice as ‘Abnormal 1 Restart the instrument to check whether the
Data:’ problem is eliminated.
53
106
AVE-772 Appendix
10 APPENDIX
10.1 Barcode Match

Note: Only use the barcode match function when working with urine analyzers!
The user must strictly comply with the rules of use in the testing process to avoid the
wrong ID and the condition that chemical and microscopic results do not match. The
instrument will match the results with barcode as top priority.
When the instrument fail to read barcode or the chemical and microscopic results’s
barcode do not match, use the barcode match function.
Fig.10-1 Barcode Match

Operations:
1. Choose the Chemistry or Microscopy ID that needs to be modified.
2. Click “Tools”- “Barcode Match” in Microscopy interface.
3. Modify the barcode ID in “Microscopy ID Modify”/“Chemistry ID Modify” and click “Modify”,
the system automatically prompts the sample ID consistent with the barcode. Click “Yes”,
the system prompts the barcode match with the sample which has the same barcode ID.
Click “OK” to achieve matching.
4. The original error sample ID will be kept in the Verification List after barcode modification
is completed. If it is not needed, delete it in right-click popup window.
Fig. 10-2 Save
107
AVE-772 Appendix
Fig.10-3 Update successful
108

AVE772 User Manul - 1

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AVE-772 User's Manual

AVE-772 Integrated Urinalysis Analyzer

AVE SCIENCE & TECHNOLOGY CO., LTD.

Hunan 410205, P.R.China

The information in this manual was correct at the time of printing.

User Manual for software version 2.2.8.2015

4.2.1.1 Urine Item (Setting) Screen ...........................................................................27

5.8.1 STAT Test ................................................................................................................49

9.1.2 Daily Maintenance ...................................................................................................82

1.1 Intended Use

1.2 How to use the User Manual

1.3 Precautions and Warnings Symbols – Definitions

CAUTION: Identifies situations that could lead to an accident or equipment failure.

CAUTION: Identifies electrical caution! Unplug before handling.

WARNING: Identifies potentially hazardous situations that could result in serious

injury to laboratory personnel.

1.3.3 Biological Warnings

Identifies potential biological hazards. Please be cautious when using, cleaning or

No. Symbol Description Location

1 Cautions User manual, nameplate

2 Cautions User manual, instrument

3 Cautions User manual, instrument

4 Cautions User manual, instrument

5 Warning User manual

User manual, instrument,

8 Power on User manual, instrument

9 Power off User manual, instrument

No. Symbol Description Location

16 Fragile Product packaging

Do not use when package

18 Stacking limits Product packaging

19 Ban rolling Product packaging

20 Upward Product packaging

21 Keep dry Product packaging

1.5 Contact Information

1.6 Limitation of Liability

1.7 Use of Third Party Computer Products

2.1 Operation Theory

2.2 Performance and Technical Index

2.3 System Components

2.3.1 System Appearance

Fig.2-2 Back view of the system

2.3.2 AVE-772’s structure

Fig.2-4 AVE-772’s structure

2.4 Software Functions

the particles as decided by the user.

2.4.1 Main Programme Screen

Function menu bar

Shortcut keys area

Fig. 2-5 Main Programme Screen

2.4.1.1 Daily Operation Main Menu

Fig.2-7 Operation main menu

2.4.1.2 Management Main Menu

Fig. 2-8 Management main menu

2.4.1.3 Quality Control Main Menu

Fig.2-9 QC main menu

2.4.1.4 Data Main Menu

Fig.2-10 Data main menu

2.4.1.5 Help Main Menu

Fig.2-11 Help main menu

2.4.2 Microscopy and Verification Screen

Fig. 2-12 Enter Microscopy Screen way 1

Fig.2-13 Enter Microscopy Screen way 2