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OFS Cap ' ' Celula General Est 2023
OFS Cap ' ' Celula General Est 2023
Editado: O. Figueroa-Santiago
Editado: O. Figueroa-Santiago
Chapter At a Glance
• Eukaryotic cells have internal membranes that
compartmentalize their functions
• The eukaryotic cell’s genetic instructions are housed in the
nucleus and carried out by the ribosomes
• The endomembrane system regulates protein traffic and
performs metabolic functions in the cell
• Mitochondria and chloroplasts change energy from one form
to another
• The cytoskeleton is a network of fibers that organizes
structures and activities in the cell
• Extracellular components and connections between cells
help coordinate cellular activities
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Overview: The Fundamental Units of Life
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tallest trees
10 m
Most cells range in size from
about 1 to 100 micrometers
visible with unaided
1m
in diameter
human eye
adult human
10 cm
1 cm chicken egg
1 mm
100 m
visible with
1 m
electron microscope
100 nm
most prokaryotic cells far away from the external
virus
environment
electron microscope
visible with special
10 nm
proteins
1 nm
diameter of DNA
Units of measurement:
double helix
1 meter (m) = 39.37 inches
1 centimeter (cm) = 1/100 m
1 millimeter (mm) = 1/1,000 m
0.1 nm 1 micrometer (m) = 1/1,000,000 m
1 nanometer (nm) = 1/1,000,000,000 m
atoms
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A Panoramic View of the Eukaryotic Cell
• A eukaryotic cell has internal membranes that partition the cell
into organelles
• Plant and animal cells have most of the same organelles
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© Pearson Education, Inc. https://www.thoughtco.com/animal-cells-vs-plant-cells-373375
Figure 6.8a
Microvilli
Golgi apparatus
Peroxisome
Mitochondrion Lysosome
Editado: O. Figueroa-Santiago
Nuclear Rough
envelope endoplasmic
NUCLEUS reticulum Smooth
Nucleolus endoplasmic
reticulum
Chromatin
Ribosomes
Central vacuole
Golgi
apparatus Microfilaments
Intermediate CYTOSKELETON
filaments
Microtubules
Mitochondrion
Peroxisome
Plasma membrane Chloroplast
10 μm
cell
Cell wall
Animal Cells
Fungal Cells
Buds
Cell Vacuole
5 μm
Nucleus
Nucleolus
Yeast cells budding Nucleus
(colorized SEM)
Human cells from lining Mitochondrion
of uterus (colorized TEM) A single yeast cell
(colorized TEM)
Cell
1 μm
Flagella
5 μm
8 μm
Cell wall
Eukaryotes
Unicellular
Plant Cells
Chloroplast Nucleus
Mitochondrion Nucleolus
Nucleus
Vacuole
Nucleolus
Chlamydomonas Chloroplast
Cells from duckweed (colorized SEM)
(colorized TEM) Cell wall
Chlamydomonas
(colorized TEM)
Eukaryotic cells have
internal membranes
that compartmentalize
their functions
Editado: O. Figueroa-Santiago
https://www.macmillanhighered.com/BrainHoney/Resource/6716/digital_first_content/trunk/test/morris2e/morris2e_ch27_2.html
Eukaryotic cells have internal membranes
that compartmentalize their functions
• The basic structural and functional unit of every
organism is
– one of two types of cells
• prokaryotic or eukaryotic
– Bacteria and Archaea
• prokaryotic cells
– Protists, fungi, animals, and plants
• eukaryotic cells
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Comparing Prokaryotic vs Eukaryotic Cells
• Basic features of all cells
– Plasma membrane
– Cytoplasm consists of all the fluid and structures that lie inside the plasma
membrane but outside of the nucleus
• excluding the nucleoid region or nucleus
• The fluid portion of the cytoplasm (cytoplasmic fluid) contains water, salts, and
organic molecules
• Most of the cell’s metabolic activities occur in the cell cytoplasm
– Chromosomes (carry genes)
– DNA (deoxyribonucleic acid)
– Ribosomes (make proteins)
– RNA (ribonucleic acid) (to guide
construction of proteins)
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What Are the Basic Attributes of Cells?
• There are two basic types of cells:
• Prokaryotic cells
– “before the nucleus”
– The simplest forms of life
• Eukaryotic cells
– “true nucleus”
– form the bodies of animals, plants, fungi, and protists
chromosome
(nucleoid region)
pili
(c) Cocci ribosomes
food granule
prokaryotic
flagellum
(b) Spirilla
capsule or
slime layer
cell wall
plasma membrane
plasmid (DNA) cytoplasm
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• plasma membrane
• is a selective barrier that allows sufficient passage of oxygen,
nutrients, and waste to service the volume of every cell
– encloses the cell and allows interactions between the cell and its
environment
– is a double layer of phospholipids
– Components: lipid, protein, and carbohydrate molecules
Inside of cell
0.1 m
Carbohydrate side chains
Hydrophilic
region
Hydrophobic
region
Hydrophilic Phospholipid Proteins
region
(b) Structure of the plasma membrane
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The Plasma Membrane
carbo-
extracellular fluid (outside)
hydrate
glycoprotein
A phospholipid bilayer
helps to isolate the
Proteins help the cell
cell's contents
communicate with
its environment
cholesterol
membrane
protein channel protein
cytoskeleton
cytoplasm (inside)
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The eukaryotic cell’s genetic instructions are housed in the nucleus
and carried out by the ribosomes
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The eukaryotic cell’s genetic instructions are
housed in the nucleus and carried out by the
ribosomes
• The nucleus contains most of the DNA in a eukaryotic cell
• Ribosomes use the information from the DNA to make
proteins
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The Nucleus: Information Central
• contains most of the cell’s genes and
is usually the most conspicuous
organelle nuclear
envelope nuclear
• is the control center of the eukaryotic nucleolus pores
ribosomes
cell
• contains three major parts
– Nuclear envelope
• is a double membrane
• lipid bilayer
– Chromatin chromatin
– Nucleolus
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The Nucleus: Information Central
nuclear
• nuclear pores envelope nuclear
nucleolus pores
• allow water, ions, and small ribosomes
molecules to pass freely
• nuclear pore complex
• Passage of proteins, pieces of
ribosomes, and RNA
• The shape of the nucleus is
maintained by the nuclear lamina
• proteins
chromatin
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1 m
Nucleus
Nucleolus
Chromatin
Nuclear envelope:
Inner membrane
Outer membrane
Nuclear pore
Rough ER
Pore
complex
Surface of nuclear
envelope Ribosome
Close-up
0.25 m
of nuclear Chromatin
envelope
1 m
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•Chromatin: a mixture of DNA and
proteins that form the
chromosomes found in the cells
•Histones are highly alkaline
proteins found in eukaryotic cell
nuclei that package and order the
DNA into structural units called
nucleosomes Editado: O. Figueroa-Santiago
Chromosomes
chromatin
chromosome
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Ribosomes: Protein Factories
• Are particles made of ribosomal RNA and protein
• Are the site of protein synthesis in the cell
• Ribosomes carry out protein synthesis in two locations
– In the cytosol
– free ribosomes
– On the outside of the ER or
the nuclear envelope
– bound ribosomes
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ribosome
mRNA
polyribosome
growing
protein
amino acid
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The endomembrane system regulates protein
traffic and performs metabolic functions in
the cell
• Components of the endomembrane system
– Nuclear envelope
– Endoplasmic reticulum
– Golgi apparatus
– Lysosomes
– Vacuoles
– Plasma membrane
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Editado: O. Figueroa-Santiago
The Endoplasmic Reticulum: Biosynthetic
Factory
• is a series of interconnected membranes
• labyrinth of interconnected flattened sacs and channels within the
cytoplasm
• All the proteins and phospholipids of cell membranes are synthesized
in the ER
• There are two distinct regions of ER
– Smooth ER
• lacks ribosomes
– Rough ER
• surface is studded with ribosomes
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Functions of Rough ER
– Secrete glycoproteins
– Distributes transport vesicles, proteins surrounded by membranes
– Is a membrane factory for the cell
– produces proteins destined for other membranes or for secretion
– Synthesizes lipids
• Hormone and cholesterol
– Metabolizes carbohydrates
– Detoxifies drugs and poisons
– Stores calcium ions
The Golgi Apparatus: Shipping and Receiving
Center
• consists of flattened membranous sacs called cisternae
• Functions of the Golgi apparatus Golgi
– Modifies products of the ER apparatus
Protein-carrying
vesicles from the ER
merge with the Golgi
apparatus
Golgi
apparatus
Vesicles carrying
modified protein leave
Editado: O. Figueroa-Santiago the Golgi apparatus
Figure 6.15-1
Nucleus
Rough ER
Smooth ER
Plasma
membrane
Editado: O. Figueroa-Santiago
Figure 6.15-2
Nucleus
Rough ER
Smooth ER
cis Golgi
Plasma
membrane
trans Golgi
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Figure 6.15-3
Nucleus
Rough ER
Smooth ER
cis Golgi
Plasma
membrane
trans Golgi
Editado: O. Figueroa-Santiago
(extracellular fluid)
(cytoplasm)
4 Completed glycoprotein
A Protein Is vesicles antibodies are packaged into
vesicles on the opposite side of
the Golgi apparatus
forming
vesicle
1 Antibody protein is
synthesized on ribosomes
and is transported into
channels of the rough ER
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Lysosomes: Digestive Compartments
• is a membranous sac of hydrolytic enzymes that
can digest macromolecules
– Lysosomal enzymes
• hydrolyze proteins, fats, polysaccharides, and nucleic acids
• work best in the acidic environment inside the
lysosome
• Some types of cell can engulf another cell
by phagocytosis; this forms a food vacuole
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(extracellular fluid) food
5 A lysosome fuses
• A lysosome fuses with the (cytoplasm) food with a food vacuole,
vacuoles and the enzymes
food vacuole and digests digest the food
the molecules
4 The enzymes
• Lysosomes also use lysosome
are packaged into
lysosomes, which
enzymes to recycle the bud from the Golgi
cell’s own organelles and apparatus
The Golgi
macromolecules 3
apparatus modifies
the enzymes as they
– autophagy pass through its
compartments
• A lysosome fuses with a
Golgi apparatus
food vacuole and
2 The enzymes are
digests food into basic packaged into vesicles
and travel to the Golgi
nutrients digestive
apparatus
enzymes
1 Digestive
enzymes are
synthesized on
ribosomes and
travel through
the rough ER
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Figure 6.13
1 m Vesicle containing
Nucleus two damaged 1 m
organelles
Mitochondrion
fragment
Lysosome Peroxisome
fragment
Digestive
enzymes
Lysosome
Lysosome
Plasma membrane Peroxisome
Digestion
Editado: O. Figueroa-Santiago
Vacuoles: Diverse Maintenance Compartments
• A plant cell or fungal cell may have one or several vacuoles, derived from
endoplasmic reticulum and Golgi apparatus
• Food vacuoles
• phagocytosis
• Contractile vacuoles
• found in many freshwater protists
• pump excess water out of cells
• Central vacuoles
• found in many mature plant cells
• hold organic compounds and water
• water balance
• store hazardous wastes, nutrients, or pigments
• provide turgor pressure on the cytoplasm to keep cells rigid
© Pearson Education, Inc. https://youtu.be/91uPoyueRjA?si=KBYfZtV1yaNZ4HaI Editado: O. Figueroa-Santiago
Contractile Vacuoles
contractile
vacuole
collecting
ducts
central
reservoir
pore
Cytosol
Central
Nucleus vacuole
Cell wall
Chloroplast
5 m
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Mitochondria and chloroplasts change energy from one form to
another
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Mitochondria and chloroplasts change
energy from one form to another
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© Pearson Education, Inc.
The Evolutionary Origins of Mitochondria
and Chloroplasts
• Mitochondria and
chloroplasts have similarities
with bacteria
– Enveloped by a double
membrane
– Contain free ribosomes
and circular DNA
molecules
– Grow and reproduce
somewhat independently in
cells Editado: O. Figueroa-Santiago
© Pearson Education, Inc.
The Endosymbiont theory
– An early ancestor of eukaryotic cells engulfed a nonphotosynthetic
prokaryotic cell, which formed an endosymbiont relationship with its host
– The host cell and endosymbiont merged into a single organism, a
eukaryotic cell with a mitochondrion
– At least one of these cells may have taken up a photosynthetic prokaryote,
becoming the ancestor of cells that contain chloroplasts
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Evidence for the endosymbiont hypothesis
1. Both mitochondria and chloroplasts are about the size of prokaryotic cells (1–5
micrometers in diameter)
2. Both have a double membrane; the outer possibly coming from the host cell and
the inner from the guest cell
3. Both have enzymes to synthesize ATP Editado: O. Figueroa-Santiago
4. Both possess DNA and ribosomes
Mitochondria: Chemical Energy Conversion
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A Mitochondrion
outer
membrane
inner
membrane
intermembrane
space
matrix
cristae
0.2 micrometer
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• Chloroplast structure includes
– Thylakoids
– Stroma
• Plastids
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A Chloroplast
outer
membrane
inner
membrane
stroma
thylakoid
channel
interconnecting
thylakoids
granum
(stack of thylakoids)
1 micrometer
– The thylakoid membranes contain the green pigment chlorophyll and other
pigments, which capture sunlight and make sugar from CO2 and water
– photosynthesis
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A Plastid
plastid
starch
globules Plants use plastids for storage
❖ are found only in plants and
photosynthetic protists
❖ They are surrounded by a
double membrane
❖ are storage containers for
various molecules
❖ pigments or starch
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Peroxisomes: Oxidation
• are specialized metabolic compartments bounded
by a single membrane
• produce hydrogen peroxide and convert it to water
• perform reactions with many different functions
• How peroxisomes are related to other organelles is
still unknown
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Figure 6.19
1 m
Chloroplast
Peroxisome
Mitochondrion
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The cytoskeleton is a network of fibers that organizes structures
and activities in the cell
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The cytoskeleton is a network of fibers that
organizes structures and activities in the cell
• is a network of fibers extending throughout the
cytoplasm
• It organizes the cell’s structures and activities,
anchoring many organelles
• Three main types of fibers make up the
cytoskeleton
– Microfilaments
– Intermediate filaments
– Microtubules
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Roles of the Cytoskeleton: Support and
Motility
Vesicle
ATP
Receptor for
(b)
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Microtubules
• Microtubules are hollow rods about 25 nm in
diameter and about 200 nm to 25 microns long
• Functions
– Shaping the cell
– Guiding movement of
organelles
– Separating
chromosomes during
cell division
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Centrosomes and Centrioles
• In many cells, microtubules grow out from a centrosome near
the nucleus
• The centrosome is a “microtubule-organizing center”
• In animal cells, the centrosome has a pair of centrioles, each
with nine triplets of microtubules arranged in a ring
Centrosome Microtubule
Centrioles
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Direction of swimming
section of cilium
0.1 micrometer
Paramecium cilium
plasma membrane
basal body
– myosin
Myosin
head
(a) Myosin motors in muscle cell contraction
– actin Cortex (outer cytoplasm):
composed of myosin
interdigitate with the
thinner actin fibers
Chloroplast 30 m
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(c) Cytoplasmic streaming in plant cells
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Intermediate Filaments
• Intermediate filaments range in diameter from 8–
12 nanometers, larger than microfilaments but
smaller than microtubules
• They support cell shape and fix organelles in
place
• Intermediate filaments are more permanent
cytoskeleton fixtures than the other two classes
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Editado: O. Figueroa-Santiago
Extracellular components and connections between cells help
coordinate cellular activities
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Extracellular components and connections
between cells help coordinate cellular
activities
• Most cells synthesize and secrete materials that
are external to the plasma membrane
• These extracellular structures include
– Cell walls of plants
– The extracellular matrix (ECM) of animal cells
– Intercellular junctions
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Cell Walls of Plants
– Cell walls are porous, allowing oxygen, CO2 and water carrying
dissolved molecules to flow easily through them
• The plasma membrane is located just beneath the cell wall
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• Plant cell walls may have multiple layers
– Primary cell wall: relatively thin and flexible
– Middle lamella: thin layer between primary walls
of adjacent cells
• pectins
– Secondary cell wall (in some cells): added
between the plasma membrane and the primary
cell wall
• Plasmodesmata are channels between adjacent
plant cells
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Figure 6.28
Secondary
cell wall
Primary
cell wall
Middle
lamella
1 m
Central vacuole
Cytosol
Plasma membrane
Plant cell walls
Plasmodesmata
Editado: O. Figueroa-Santiago
The Extracellular Matrix (ECM) of Animal
Cells
•Animal cells lack cell walls but are covered by an elaborate extracellular
matrix (ECM)
– Functions: Support, adhesion, movement, regulation
•The ECM is made up of glycoproteins such as collagen, proteoglycans,
and fibronectin
Collagen Polysaccharide
EXTRACELLULAR FLUID
•ECM proteins molecule
Carbo-
bind to receptor Proteoglycan
complex hydrates
Micro- CYTOPLASM
filaments
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Cell Junctions
• Neighboring cells in tissues, organs, or organ
systems often adhere, interact, and
communicate through direct physical contact
• Intercellular junctions facilitate this contact
• There are several types of intercellular junctions
– Plasmodesmata
– Tight junctions
– Desmosomes
– Gap junctions
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Plasmodesmata in Plant Cells
• are channels that perforate plant cell walls
• Through plasmodesmata, water and small solutes
(and sometimes proteins and RNA) can pass from cell
to cell
Cell walls
Interior
of cell
Interior
of cell
0.5 m Plasmodesmata Plasma membranes
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Tight Junctions, Desmosomes, and Gap
Junctions in Animal Cells
• At tight junctions (uniones estrechas), membranes
of neighboring cells are pressed together,
preventing leakage of extracellular fluid
• Desmosomes (anchoring junctions) fasten cells
together into strong sheets
• Gap junctions (uniones en hendiduras)
(communicating junctions) provide cytoplasmic
channels between adjacent cells
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Figure 6.32
TEM
0.5 m
Tight junction
Intermediate
filaments
Desmosome
TEM
1 m
Gap
junction
Ions or small
molecules
Space
TEM
between cells
Extracellular
Plasma membranes matrix
of adjacent cells Editado: O. Figueroa-Santiago 0.1 m