CD4 T Cell Differentiation +

CD69
CD94

Co Ad
Surface Markers Thymus CD150 (SLAMF1)
CD160 (Ms)
CD161 (Ms)
CD314 (NKG2D) CD44
IL-15 Ly-108 CD49b
TIM-3 (CD366) CD56 (N-CAM)
Co Ad

Regulation of
In

ra Cy
rk
er

cel

NKT
t

Ch lular Ma
immune responses, CD45RA
immune tolerance CD45RB
VISTA (PD-1H)
CD44lo
CD62L (L-Selectin)
Effector Co Ad Cy
Cytokines IL-15Rα
CD39 PLZF
CD45RA CD73
CD152 (CTLA-4)
Surface Markers
Naïve
CD103

APC
Co Costimulatory or Coinhibitory Molecules
CD279 (PD-1)
Ad TCR Ligation Initiation of
IL-4
TIM-3 (CD366) (Ms)
immunity,
Th
Co + IL-17A
Adhesion Molecules
Ad
Costimulation IFN-γ regulation

nTreg
Cy Cytokines or Cytokine Receptors

Fo x P 3

AT 5
Ch Chemokine Receptors Cy
Ch CD127

ST
Activators Cy CD184 (CXCR4)
Ch He CD197 (CCR7)
•
3 CD25hi

•
CD197 (CCR7) lio s d
• Sma CD127lo
CD134 (OX-40) IL-2
Inhibitors Immunity
Th22
CD137 (4-1BB) CD69hi

TGF-β
CD304 (Nrp1)
FolR4/FR4 (Ms)
CD71
CD98hi
to microbial
Hu = Human IL-10 GARP HLA-DR pathogens
TNF
IL-6,
IL-35 GITR LAG-3 (CD223)
Ms = Mouse LAP TIGIT CD44hi Ch Cy
APC = Antigen Presenting Cell TIM-1 (CD365) Ad CD194 (CCR4) AhR
TIM-3 (CD366) CD25hi CD140 (PDGFR)
CD196 (CCR6)
VISTA (PD-1H) Cy CCR10
Co
IL-22
TGF-β
Activated
TNF

CD152 (CTLA-4)
CD223 (LAG3) Th IL-1, I
L-6, IL CD161 (NK1)

Fo

B
TGF -β -21, IL

B7
Acid, -23, T
CD279 (PD-1) CD39 CD278 (ICOS)
3
BT

xP
o ic •K GF-β
Retin
TIM-3 (CD366)
i-6 7 • Z
CD73 TIM-3 (CD366)
Co CD103 IL-2, Co
Ad

R
iTreg

f a
IFN-γ

OR

IR F 4 • c- M
, IL-2,
Regulation IL IL-4, I

γt
Th17
-2 L-12,
,I

• RORα4
of tolerance IL-1 IL-27

IL-
L-1
Fo x P

hR

IL-21 13
2(

2, I
p7
•A

IL-23 CL 0)

L-4
3

f•
Cy ,I
Sm T C L-1
5

1,
•

S TA

, IL

•
TA

at
Ch ad3 • CD25 hi
2 8, •B

S
T3
Variable CD127lo L- • Runx1

-25
I IL Ch Cy
expression CD134 (OX-40) , -2
-6 7, CD126 (IL-6Rα)

, IL
IL CD26 CD194 (CCR4)
GF-β

CD137 (4-1BB) IL -4 RA

IFN
CD94 CD196 (CCR6) IL-17A IL-13Rα1
,I NK

-31
IL-10 FolR4/Fr4 (Ms)
L-1 L CD278 (ICOS) IL-17F IL-21R

-γ,
IL-35
IL-4, T

GARP 0,

, IL
IL-17A/F IL-23R
IFN-γ
Tim-3 (CD366)
TGF-β IL

TG
GITR
-2 IL-21

-33
Co
LAP 1

F
IL-22

-β
Immunity to

, TS
CD84 (SLAMF5) IL-24
CD150 (SLAMF1)
extracellular

LP
IL-26 (Hu)
CD200 (OX-2) TNF

Th1
CD272 (BTLA)
CD278 (ICOS)
CCL20 (MIP-3α) bacterial and
CD279 (PD-1) fungal infections
TIGIT CD57

T-b

nx3
Co Ad Tim-1 (CD365)
Co

et

Ru
ST
4•

•
AT 1
• S T AT

GA

af
Ch Cy

IR F 4 • c- M
T
Tfh
CD183 (CXCR3)

A 3 • S T AT 5 •
Th2
CD178 (FasL)
CD195 (CCR5) CD212 (IL-12Rβ2)
IFN-γ
TGF-β LT-α CD218a (IL-18Rα)
TNF IL-27Rα
Ch NOTCH3
l-6 Perforin
Bc

T3

1•
G AT A 3 ( e a r l

Cy
.1 • IR F4

TA ST RANKL

Th9
CD185 (CXCR5) • M Granzyme A
AF • S F

I-
CD126 (IL-6Rα) AT
CD153 (CD30L) Ch 6 • M A F • G Cy Granzyme B
CD30 Granulysin (Hu)
CD154 (CD40L) CD184 (CXCR4)
IFN-γRβ Immunity to
T-cell help IFN-γ CD304 (Nrp1)
PU

CD193 (CCR3)
IL-4 IL-21R CD194 (CCR4)
IL-4 IL-17RB
viruses,
for B cells •
y)

T 6 Cy
IL-33Rα
•

IL-17A IL-5
SM
IL-17F A D s • S TA VDR (Ms)
CD198 (CCR8)
CD294 (CRTH2)
IL-6 NOTCH1 intracellular
IL-21 IL-10
IL-13
NOTCH2
bacteria,
IL-9 IL-31 and parasites
IL-10 Immunity to
CCL17 (TARC) Immunity to
CCL22 (MDC) extracellular
extracellular
TGF-β
parasites
T cells play central roles in cell-mediated and humoral immunity. Several
parasites
different subsets of T cells have been discovered, each with distinct functions.
Each subset has a characteristic cytokine signature that can be measured at the
single-cell level using multicolor flow cytometry, combining surface phenotyping
and intracellular staining of cytokines, transcription factors, or signaling molecules.
Additionally, multiple soluble cytokines or signaling molecules can be quantitated
simultaneously using BD™ Cytometric Bead Array (CBA) technology.
Other complementary technologies to further the research in the T cell area include
BD OptEIA™ ELISA reagents for cytokine quantitation and BD™ ELISPOT reagents
for determining the frequency of cytokine production in cells.
BD Life Sciences
bdbiosciences.com/tcell
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
© 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Biosciences
23-10639-03 bdbiosciences.com