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  • Vista Do 2020 - Anais Do II Congresso Internacional em Ciências Da Saúde I Congresso Internacional em Biotecnologia I Congresso Internacional em Cuidado Primário À Saúde

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    Vista do 2020_ Anais do II Congresso Internacional em Ciências da Saúde I Congresso Internacional em Biotecnologia I Congresso Internacional em Cuidado Primário à Saúde

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    8 views1 page

    Vista Do 2020 - Anais Do II Congresso Internacional em Ciências Da Saúde I Congresso Internacional em Biotecnologia I Congresso Internacional em Cuidado Primário À Saúde

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    flaviarozabiomedica

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    ENN GOE MO meals AES Ry UD )3 REVISTA UNIMONTES CIENTIFICA (ISSN 2236-5257) EFFECT OF SECONDARY METABOLITES FROM Lactococcus lactis IN GROWTH, in vitro, OF RESIDENT PROCARIOTS IN GASTROINTESTINAL TRACTS Flavia Roza Silva 1 Victéria Lima Cavalcanti Starling 2 Ronize Viviane Jorge Brito 3 Sergio Avelino Mota Nobre 4 Leticia Antunes Athayde Souza S Introduction: studies have shown the influence of Lactococcus lactis on the abundance and diversity of the intestinal tract microbial population of mice treated with alcohol acting as a probiotic. However, the effect of secondary metabolites from Lactococcus lactis over this microbial population is not known. It is important to know alternative intestinal microbiota modulation strategies capable of attenuating or reverting the effects generated by dysbiosis, because they can cause clinical implications, Objective: evaluate the effect of Lactococcus lactis’ extracellular secondary metabolites on its in vitro growth of bacteria residing in the gastrointestinal tract. Materlals and Method: Species Lactococcus lactis NCDO 2118 was wilized and the extracellular secondary metabolites were obtained through a 22 hour culture. To evaluate these metabolites’ action, the microdilution sensitivity test was performed utilizing a suspension composed of Escherichia coli ATCC 8739, Enterococcus faecalis ATCC 4083, Lactococcus lactis NCDO 2118, Enterococcus sakazakit ATCC 29544, Klebsiella oxytoca, Salmonella enteritidis, Lactobacillus rhamnosus, E. coli from intestinal cleansing of an alcohol- treated animal and another strain of £. co/i from intestinal cleansing of an alcohol-treated animal alongside with the Lactococcus Jactis’, in accordance to The Clinical and Laboratory Standards Institute. After the incubation, the colony counting plating was performed and the results were expressed in percentage form with the mean = standard error of the mean, Results: The extracellular metabolites have shown various intensities of inhibitory action over the investigated microorganisms. The microorganisms have shown the following intensities of inhibition: Salmonella enteritidis (54.4%), Lactobacillus rhamnosus (51,9%), Enterococcus sakazakii ATCC 29544 (47.9%), Escherichia coli from the intestinal cleansing of an aleohol-treated animal and Lactococeus lactis (46,7%), Escherichia coli ATCC 8739 (42,4%), Escherichia coli from the intestinal cleansing of an alcohol-treated animal (41.6%), Klebsiella oxytoca (31.1%), Lactococcus lactis NCDO 2118 (24,1%) and Enterococcus faecalis ATCC 4083 (19.6%). Conclusion: The Z. /actis extracellular metabolites inhibited the growth of bacteria from the Enterobacteriaceae family and from those who produce lactic acid, with acknowledged probiotic activity. It is suggested that these metabolites can be effective on the biological control of these microorganisms, thus modulating the intestinal microbiota. Keywords: Lactococcus lactis; Extracellular secondary metabolites; Enterobacteriaceae; Lactic acid bacteria + Graduanda em Biomedicina, Faculdade de Sade Tbituruna - FAST, Montes Claros, Minas Gerais Brasil. E-mail: rozaflavia@gmail.com,

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