Effect of Auricularia auricula-judae (Black Wood Ear) on

Solanum lycopersicum (Tomato) Shelf Life: A Potential on

Alternative to Synthetic Preservatives

An Undergraduate Thesis Proposal Presented to

The Faculty of College of Arts and Sciences
West Visayas State University
Iloilo City

In Partial Fulfilment
Of the Requirements for the Degree
Bachelor of Science in Biology
Medical Biology Track

By

Lowella Rose C. Donguines

Hannah May C. Fulgencio

March 2023
 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

Effect of Auricularia auricula-judae (Black Wood Ear) on

Solanum lycopersicum (Tomato) Shelf Life: A Potential on

Alternative to Synthetic Preservatives

An Undergraduate Thesis Proposal for the degree

Bachelor of Science in Biology
Medical Biology

By
Lowella Rose C. Donguines
Hannah May C. Fulgencio

Approved:

______________________________________

SATURNINO LUKE A. SOBREVEGA, M.Sci (Adviser)

_____________________________________

CHARRIZ AMOYAN, M.Sci (Student Research Coordinator)

_____________________________________

JEANNEMAER GENEVIVE Y. FIGUERAS, Ph. D (Chair, Biological

Sciences Department)

_____________________________________

Anita Estela M. Monroy, Ph.D (Dean)

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

Acknowledgments

This study was only completed with the assistance of

these encouraging people who assisted and guided us

throughout the research project. The researchers wish to

express their sincerest gratitude to the following

individuals:

To Mr. Saturnino Luke Sobrevega, our ever-supportive

and proficient Research adviser to him, the researchers

extend our gratitude for his unending support, awe-inspiring

advice, and, most significantly, for the overwhelming

enthusiasm towards the research study.

To Ms. Shenly Gazo for the help she willingly gave us

throughout our journey to achieving the success of our

study.

To Mrs. Cynthia Limsiaco for giving her all-embracing

appraisal to make this study an accomplishment.

To our Family, especially our Parents, for serving as

our inspiration and being supportive of this virtue. Since

initiating this endeavor, they have provided us with

financial, physical, and emotional support and have always

been there when we needed them.

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

To our Friends and Classmates, who played a significant

role in this study, for assisting us in completing this

study successfully and for being there to inspire and

motivate us to the extent that we productively completed our

study.

And most especially to our Almighty Savior, our God,

for making everything possible and granting us the wisdom,

knowledge, fortitude, and perseverance to complete this

study. We will never be able to achieve this level of

success without you. Thank you for not abandoning us.

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

Abstract

Solanum lycopersicum is a highly perishable fruit that

deteriorates after ripening. Chemical preservatives extend

the shelf life of fruits. However, the accumulation of these

in tissues has led to health risks. This paved the way for

searching for a new species that could serve as a natural

preservative. Several pieces of research showed that

Auricularia auricula-judae extracts have antimicrobial and

antioxidant potential against microbial agents. Thus, this

study aims to evaluate its efficacy in prolonging the shelf

life of S. lycopersicum. The study has six (6) set-ups, four

(4) of which were the different concentrations of A.

auricula-judae extracts, and two (2) were the positive and

negative control. The efficacy of each treatment was studied

by measuring and comparing the initial and final weight of

each tomato before and after the application of treatments,

respectively. Results showed that A. auricula-judae extract

at 100% concentration had the highest preservation rate and

had no significant difference with the synthetic

preservative, with a p-value of 0.9244, greater than 0.05.

Keywords: mushroom, extracts, A. auricula-judae, S.

lycopersicum, shelf life, preservatives

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

TABLE OF CONTENTS

Page

Title Page ii

Approval Page iii

Acknowledgment iv

Abstract vi

Chapter

I BACKGROUND OF THE STUDY 3

Conceptual Framework 7

Research Paradigm 8

Statement of the Problem 10

Hypothesis 11

Definition of Terms 12

Delimitation of the Study 15

Significance of the Study 16

II REVIEW OF RELATED LITERATURE

Food Preservation 20

Mushroom Extracts 22

Auricularia auricula-judae 25

Solanum lycopersicum 27

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

III METHODOLOGY

Research Design 32

Data-gathering Procedure 33

Gathering and Preparing Raw Materials 33

Gathering of Auricularia auricula-judae 33

Identification of A. auricula-judae 34

Gathering of Solanum lycopersicum 34

Extraction of Mushroom 35

Preparation of Synthetic Preservative Solution 35

Preparation of Set-ups 36

Application of Different Treatment 38

Data Collection 38

Statistical Analysis 40

Descriptive Data Analysis 40

Inferential Data Analysis 41

IV RESULTS AND DISCUSSION

Descriptive Statistical Analysis 43

Inferential Data Analysis 46

V SUMMARY AND FINDINGS, CONCLUSIONS,

IMPLICATIONS AND RECOMMENDATIONS

Summary 50

Findings 51

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

Conclusion 53

Implications 54

Recommendations 54

References 55

Appendices 69

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

LIST OF TABLES

Table Title Page

1 The Set-up of the Study 37

2 The mean weight loss and 45
the standard deviation of the
Solanum
lycopersicum
3 One-way ANOVA of Different 47
Concentrations and Positive
Control
4 One-way ANOVA of Different 48
Concentrations and Negative
Control

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

LIST OF FIGURES

Figure Title Page

1 The Relationship between the Independent 9

and

Dependent Variables of the Study

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

LIST OF APPENDICES

Appendix Title Page

A DOCUMENTATION OF THE EXPERIMENT 69

PROPER

Gathering of the materials 69

Sodium Benzoate (Synthetic 70
Preservative)
Washing of Solanum lycopersicum 70

Weighing of Solanum lycopersicum 71

Weighing of sodium benzoate 72

Boiling of A. auricula-judae 72

Varying concentrations of A. 73
auricula-judae, the benzoate
solution and water (from left to
right)

Application of different 73
Treatments
Tomatoes on day 7 74

B DATA CALCULATION AND ANALYSIS

Recorded initial and final weight 75

of tomatoes
One-way ANOVA of Different 76
Concentrations and the Positive
Control
One-way ANOVA of Different 77
Concentrations and Negative

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 West Visayas State University
COLLEGE OF ARTS AND SCIENCES
Iloilo City

Control

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