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Universi, &
sneering Barth Resources Malaya!
Faculty of iil Engineering & Bard VU PAHARS
Universiti Malaysia Pahanss
EMICAL OXYGE!
DEMAND (BOD)
ENY-01: BIOC!
sesetolner meer?
due tution strength of domestic and industrial wastes in terms o
ine the pol
1) To determine the pol :
: cexygen that they will requir in oxidation Process.
2) Todetermine the constant rate valu K.
APPARATUS
1) BOD bottles
2) BOD incubator (Temperature = 20°C)
REAGENTS
Phosphate buffer
Dissolve each 8.5 g KH:POs, 21.75 g KsHPOs, 33.4 g NaHPO,7H20 dissolved 1.7 g NH
in distilled water to prepare 1 litter of solution. Adjust pH 7.2.
‘Magnesium Sulphate
Dissolve 27.5 g MgSOx.7H20 in distilled water to prepare 1 litre of solution.
Calcium Chloride
Dissolve 27.5 g of anhydrous CaCl in distilled water to prepare | litre of solution.
Ferric Chloride
Dissolve 0.25 g FeCls 6H20 in distilled water to Prepare | litre of solution.
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a hts NS Unveren
Malaysia
1) Prepare dilution Water in a. glass container by bubbling co
water for about 30 minutes. Add 1 ml each of, ee arin
calcium chloride and ferric chloride for each litre of dilution vm when su
ter & mix thy
of Coil Engineering & Earth Resources
istlled
phate,
Neutralise the sample to pH around 7.0 by using I N NaOlt or H,S0, roughly
‘80H or H2S0.,
Since the DO in the sample is likely tobe exhausted, it is usually neces
ecessa
a suitable dilution of the sample according to the expected BOD ran; FY o prepare
given below for the dilution of the sample ge. See the table
‘Volume of sample (mL) a
os 30 000-105 000
095 12000-42000
a 6000—21 000
es 3 000— 10500
030 1200-4200
109 6002 100
20 300-1 050
a 130420
10.00 Ea
20.00 30-105
30.00 12-42
100.00 6-2
fontent thoroughly. Fill the water into the
4) Prepare dilutions in the bucket, mix the « hy
ith oxygen meter and after that keep itn
BOD bottles. Determine the DO content w'
the BOD incubator at 30°C.
5) Determine DO content in the sample bottles for day 1, 2and3.
CALCULATION
D0,-DO,
BOD,
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“OD =biochemical oxyeen demand, mg/L
po, = initial DO ofthe dilated waste water sample about 15 min. ater
"preparation, m/l
po, =final DO ofthe diluted wastewater sample after incubation for ¢ days, moj,
p= dilution factor
Vs
Vs+Vow
From the experiment results of BOD for various values of t
1) Calculate [time/BOD,]"® for each day.
2) Plot [¥ BOD]J"® versus t
DISCUSSION
1) Plot (vBOD)"® versus
2) Fromthe graph, determine the intercept (A) and slope (B) and calculate the K value
where K =2.61 (BIA).
3) Calculate value of BODs at 20°C.
CONCLUSION
RESULT
Biochemical Oxygen Demand (BOD)
pH value
NaOH or HCI volume
De
Time, t o DO. BOD,
(mgL) (gL) ao (BoD)
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