 The following is single strand for the gene coding

for a bacteria peptide.

5’-ATGAGGCCTAAATCGTATGAATTTCACACG-3’

i. What is the complementary strand for this gene.

3’-TACTCCGGATTTAGCATACTTAAAGTGTGC-5’

ii. If this complementary strand is used as the

template in the Transcription what will be the
sequence of mRNA.

5’-AUGAGGCCUAAAUCGUAUGAAUUUCACACG-3’
iii. If the mRNA is translated successfully, what
will be the primary sequence of the peptide?

Met-Arg-Pro-Lys-Ser-Tyr-Glu-Phe-His-Thr

iv. What is the net charge of this peptide at pH 2

and at pH 10.

v. If the peptide is digested with chymotrypsin

how many fragments will be produced.

Met-Arg-Pro-Lys-Ser-Tyr
Glu-Phe
His-Thr
3 fragments
vi. If a restriction enzyme MmoI, with restriction
site 5’-AATT-3’ is applied to double stranded
DNA of the gene, how many fragments will be
produced

5’-ATGAGGCCTAAATCGTATGAA-3’ 5’-TTTCACACG-3’
3’-TACTCCGGATTTAGCATACTT-5’ 3’-AAAGTGTGC-5’

vii. If a restriction enzyme BmaIII, with restriction

site 5’-GAATTC-3’ is applied to double stranded
DNA of the gene, how many fragments will be
produced.

5’-ATGAGGCCTAAATCGTATGAATTTCACACG-3’
3’-TACTCCGGATTTAGCATACTTAAAGTGTGC-5’
viii. If the gene mutates at position +18 and T is
replaced by A, what will happen to the translated
protein. And what is the type of this mutation.

5’-ATGAGGCCTAAATCGTAAGAATTTCACACG-3’
3’-TACTCCGGATTTAGCATTCTTAAAGTGTGC-5’

5’-AUGAGGCCUAAAUCGUAAGAAUUUCACACG-3’

Met-Arg-Pro-Lys-Ser-Stop

Nonsense mutation
ix. If the gene mutates at position +12 and A is
replaced by C, what will happen to the translated
protein. And what is the type of this mutation.

5’-ATGAGGCCTAACTCGTATGAATTTCACACG-3’
3’-TACTCCGGATTGAGCATACTTAAAGTGTGC-5’

5’-AUGAGGCCUAACUCGUAUGAAUUUCACACG-3’

Met-Arg-Pro-Asn-Ser-Tyr-Glu-Phe-His-Thr

Missense Mutation
x. If the gene mutates at position +21 and A is
replaced by G, what will happen to the translated
protein. And what is the type of this mutation?

5’-ATGAGGCCTAAATCGTATGAGTTTCACACG-3’
3’-TACTCCGGATTTAGCATACTCAAAGTGTGC-5’

5’-AUGAGGCCUAAAUCGUAUGAGUUUCACACG-3’

Restriction site for MmoI is lost.

Met-Arg-Pro-Lys-Ser-Tyr-Glu-Phe-His-Thr

Silent mutation
xi. If the nucleotide C position +7 is deleted and not
replaced, what will happen to the translated
protein. And what is the type of this mutation.

5’-ATGAGG-CTAAATCGTATGAATTTCACACG-3’

5’-AUGAGG-CUAAAUCGUAUGAAUUUCACACG-3’

Met-Arg-Leu-Asn-Arg-Met-Asn-Phe-Thr

Frameshift Mutation