Professional Documents
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Installation Guide
71500049404/Revision B
Trademarks
Waters is a registered trademark of Waters Corporation, and e-SAT/IN,
Empower, SAT/IN and “THE SCIENCE OF WHAT’S POSSIBLE.” are
trademarks of Waters Corporation.
Other registered trademarks or trademarks are the sole property of their
owners.
ii
Customer comments
Waters’ Technical Communications department invites you to tell us of any
errors you encounter in this document or to suggest ideas for otherwise
improving it. Please help us better understand what you expect from our
documentation so that we can continuously improve its accuracy and
usability.
We seriously consider every customer comment we receive. You can reach us
at tech_comm@waters.com.
Contacting Waters
®
Contact Waters with enhancement requests or technical questions regarding
the use, transportation, removal, or disposal of any Waters product. You can
reach us via the Internet, telephone, or conventional mail.
Safety considerations
Some reagents and samples used with Waters instruments and devices can
pose chemical, biological, and radiological hazards. You must know the
potentially hazardous effects of all substances you work with. Always follow
iii
Good Laboratory Practice, and consult your organization’s safety
representative for guidance.
Safety advisories
Consult Appendix A for a comprehensive list of warning and caution
advisories.
Applicable symbols
Symbol Definition
Manufacturer
iv
Intended use of the Waters e-SAT/IN module
Waters designed the e-SAT/IN module to translate analog signals to digital
signals for data collection. You can operate the device with instruments used
to analyze various types of compounds. The e-SAT/IN module is for research
use only.
Calibrating
To calibrate LC systems, follow acceptable calibration methods using at least
five standards to generate a standard curve. The concentration range for
standards should include the entire range of QC samples, typical specimens,
and atypical specimens.
When calibrating mass spectrometers, consult the calibration section of the
operator’s guide for the instrument you are calibrating. In cases where an
overview and maintenance guide, not operator’s guide, accompanies the
instrument, consult the instrument’s online Help system for calibration
instructions.
Quality-control
Routinely run three QC samples that represent subnormal, normal, and
above-normal levels of a compound. Ensure that QC sample results fall within
an acceptable range, and evaluate precision from day to day and run to run.
Data collected when QC samples are out of range might not be valid. Do not
report these data until you are certain that the instrument performs
satisfactorily.
ISM classification
v
EC authorized representative
Telephone: +44-161-946-2400
Fax: +44-161-946-2480
Contact: Quality manager
vi
Table of Contents
Copyright notice ................................................................................................... ii
Trademarks ............................................................................................................ ii
Customer comments ............................................................................................ iii
Table of Contents ix
x Table of Contents
1 Introduction
Contents
Topic Page
About the e-SAT/IN module 1-2
Features 1-3
1-1
About the e-SAT/IN module
The Waters e-SAT/IN (Ethernet-SATellite INterface) module is a single-time
base, dual-channel, analog-to-digital (A/D) converter. A data acquisition
system (Empower™) can collect data from either a single channel or both
analog channels. The data start and stop times are identical for both channels.
The e-SAT/IN module uses a dedicated local area network (LAN) to
communicate to the chromatography data acquisition computer. You can
control the e-SAT/IN module using Waters Empower software.
Waters e-SAT/IN
RELAY
START
DATA
TTL1
TTL2
INJ 1 0
1-2 Introduction
• Internal storage buffer (225 K) with a capacity of 1 minute of data when
both channels are operating at a data rate of 100 samples per second.
• One Ethernet 10 base-T connector for communication.
• Two analog data input channels.
• One BCD input port.
• Three programmable event outputs. Two use TTL and one uses relay
event output.
• Self-test and internal calibration at power-on.
• Power-on self-test status indicator (LED).
• Data status indicator (LED).
• Dedicated external voltage autosensing power supply.
Features
This section describes the features of the e-SAT/IN module.
Baseline noise
As analysis times decrease, higher data sampling rates are required to
accommodate narrower peaks. The e-SAT/IN module features new technology
and a design that reduces chromatographic baseline noise at all its sampling
rates.
Chromatography data requirements have advanced allowing shorter run
times, higher data rates, and lower limits of detection. Technological advances
with the latest A/D converters and surface-mounted device design have shown
that noise can be significantly reduced at higher data rates if the data
sampling rate is synchronized to the “local” power line input frequency. For
optimum performance, you must select a line frequency, then select a data
rate from a list of values appropriate to the line frequency.
Sampling rates
To minimize baseline noise, you can optimize sampling rates to the frequency
of the local power line environment, either 50 or 60 Hz.
The Instrument Method Editor contains a field to select the power line
frequency. This value does not affect the actual power (line frequency)
Features 1-3
supplied to the instrument, but will allow you to select optimum sampling
rates that are set to the selected frequency.
When using data rates above 10 samples per second, a method developed for
use throughout global locations where power source frequencies differ can be
optimized so that either:
• The method is the same in all geographies, but may have slightly higher
baseline noise in some areas.
• The method has the lowest baseline noise in all geographies, but will
require different sampling rates.
1-4 Introduction
e-SAT/IN module provides up to 24-bits of A/D conversion (depending on the
sampling rate).
Signal flow
Digital data
Analog packet
Monitor Mouse
signal
Waters 2487
Dual l Absorbance
Detector
D
Detector A/D
Ethernet Keyboard
e-SAT/IN Acquisition
Detector module CPU computer
BCD input
The BCD (binary coded decimal) input records the BCD output from
chromatography autosamplers. Many chromatography autosamplers have
BCD output capability to document the vial number of the injection that was
made. Binary coded decimal input signals numbered 0 to 99 are bundled into
the data packet and sent using Ethernet to the acquisition computer. The
autosampler output should be compatible with the e-SAT/IN BCD input
configuration. Refer to page 2-10, and the table titled “BCD connection
assignments” on page 2-11.
Features 1-5
Programmable event outputs
The e-SAT/IN module provides three programmable event outputs as shown
in the table below
Power-on self-tests
After powering on the e-SAT/IN module, it performs self-tests to determine
that the unit is functional. When the power-on self-test sequence is completed
successfully, the green LED indicator shows that the unit is requesting an IP
address. Refer to page 3-2 for indicator details.
IP address sequence
The green LED indicator shows that the e-SAT/IN module is requesting IP
configuration information by blinking. After the module receives its IP
configuration information, the LED remains on and the e-SAT/IN module is
ready for use. Refer to page 3-2 for indicator details.
1-6 Introduction
External power supply
The Waters e-SAT/IN module uses an external power supply that is provided
with the device. The power supply automatically adjusts to the AC input line
voltage, and provides the proper DC voltage to the e-SAT/IN module. A
separate AC power supply cable is provided for the country in which it will be
installed.
The DC power cable connector is designed with a locking mechanism that
latches to the power input connector in the e-SAT/IN module. This eliminates
accidental disconnection of the power cable during operation. You must
mechanically unlatch the connector when disconnecting it. Refer to the figure
“e-SAT/IN module and power supply kit” on page 2-12.
Caution:
• Failure to unlatch the DC power connector before disconnecting can
damage the connector.
• Use only a Waters e-SAT/IN power supply module to power your
e-SAT/IN module. Substitute power supplies can damage the
e-SAT/IN module and void your warranty.
Features 1-7
1-8 Introduction
2 Installing the Module
Contents
Topic Page
Selecting the site 2-2
Making electrical connections 2-2
Installing the network interface card 2-2
Connecting analog cables 2-7
Connecting event cables 2-8
Connecting the BCD cable 2-10
Connecting the power supply 2-11
2-1
Selecting the site
Install the e-SAT/IN module in a laboratory location that meets the following
conditions:
• Install the e-SAT/IN module up to 100 meters (320 feet) from the
acquisition computer or the Ethernet switch.
• Locate the e-SAT/IN module within 2 meters (6.6 feet) (maximum
analog cable length) from the detector(s) to which it is to be connected.
• Locate the e-SAT/IN module within 1.5 meters (4.9 feet) (maximum BCD
cable length) from the autosampler to which it is to be connected.
• Do not install the e-SAT/IN module and the associated network
components where they can be subjected to fluidic leaks.
• Do not install the e-SAT/IN module close to devices that emit high levels
of electromagnetic interference (EMI) or radio frequency
interference (RFI).
• Use only grounded AC power receptacles to turn on the e-SAT/IN
module power supply.
• When using an Ethernet switch (multiple-connection terminal) in a
multiple-module system configuration, set the Ethernet switch in a
central physical location within the system.
• Install the e-SAT/IN modules in an equipment rack using DIN rails.
Ethernet connections
The e-SAT/IN module communicates with the acquisition computer through
the dedicated local area network (LAN). At the acquisition computer, an
installed network interface card (NIC) printed circuit board (customer
supplied) provides the interface that allows communication to occur.
You must install the e-SAT/IN module software driver in the acquisition
computer before the computer will recognize the presence of the e-SAT/IN
module. Refer to the software installation instructions that accompanies the
Empower Software Instrument Driver disk.
Modular
10 base-T
coupler
Ethernet
crossover
cable
Network
interface card
e-SAT/IN module
10 base-T
Ethernet
cable Acquisition computer
Network
interface card
Acquisition computer
10 base-T
Ethernet cable
Output
Ethernet switch
Analog
cables
Detectors
Modular
10 base-T
coupler
Ethernet
crossover
cable
Network
interface card
e-SAT/IN module
10 base-T
Ethernet
cable
busLAC/E
board
busSAT/IN module
Serial
cable
I/O distribution
box
Acquisition computer
Channel 1 Channel 2
2. Connect the other end of the analog cable to the proper contacts on a
detector (see the operator’s guide for the detector). Note the positive (+)
and negative (−) markers on the two wires. Connect both wires.
Place the detector up to 2 meters (6.6 feet) from the e-SAT/IN module
(maximum analog cable length).
Tip: The e-SAT/IN module signal input range is –0.25 to 2.25 V. Detector
signals greater than 2.25 V produce a saturated signal.
3. To connect a second analog cable to the Channel 2 connector on the
e-SAT/IN module, repeat steps 1 and 2.
Event in connections
The e-SAT/IN module can accept either a TTL switch closure or pulsed inject
start signal from a manual injector or autoinjector. The inject start signal
triggers the e-SAT/IN module to start collecting and converting data.
8 IN (−)
1. Because both Channel 1 and 2 share the same timebase, both channels will be triggered at
the same time.
1 2 3
4 5 6
7 8
Remove
connector
To injector
Ethernet
connector
Power Data
BCD
Power
supply
connector
BCD cable
The table below lists BCD connection assignments and their binary values.
e-SAT/IN
module
Label
Power supply
Power cable
(country specific)
Slide Connector Back to Unlock
Contents
Topic Page
Powering on the module 3-2
Configuring the e-SAT/IN module 3-3
Troubleshooting 3-17
3-1
Powering on the module
When all connections have been made, power on each detector connected to
the e-SAT/IN module and any devices connected to the external event
terminals. Refer to the specific guides for each device for power-on procedures.
The e-SAT/IN module has a power switch on the front panel. The switch is a
pushbutton switch that controls the power from the power supply.
• To turn the device on (1 ), press the switch once. The LED power
indicator will light.
• To turn the device off (0 ), press the switch once. The switch will
return to the extracted position, switching off the power to the unit. The
LED power indicator will extinguish.
Successful power-on
When the power-on and POST sequence are successful and the module has
obtained an IP address, the green LED remains lit.
Power-on problems
Refer to “Troubleshooting” on page 3-17, for information.
To configure the e-SAT/IN module using the Instrument Method Editor in the
Empower Pro interface:
1. Log in to Empower, then click Browse Project. The Browse Project dialog
box opens.
Enabling events
Enabling an event output generates a contact closure that can initiate a
process on another device (at a specified time after the e-SAT/IN event clock
starts). The contact closure can control operation of a fraction collector or
chart recorder, or activate solvent/column switching valves.
You can program event outputs in the Events table rows. In each row, specify
the time that the event is to occur, the event function (On, Off, Pulse, or
Repeat Pulse), and the duration of pulsed events.
Tip: Event outputs do not provide power to run the external device. You must
use an appropriate power supply to run the device.
3. Access the instrument lists by selecting View > Method > Instrument.
Instrument
selection
control
Troubleshooting
During power-on and operation, the e-SAT/IN module indicates its status
using the LEDs on the front panel. Refer to the following table for a summary
of the LED indicators.
Troubleshooting 3-17
Summary of LED indicators (Continued)
Power-on problems
If the power LED does not light, check the following:
• Power supply is connected to a functional grounded AC voltage source.
• Power supply cable is properly attached to the e-SAT/IN module.
• e-SAT/IN module power switch is set to the on (1) position.
System troubleshooting
To perform basic system troubleshooting:
1. Examine the system, checking the simple things first. Is something
obvious causing the problem? For example, is an instrument unplugged
or improperly connected?
2. Compare the current system operation with the way the system
operated before the problem started. To help you identify normal
operating conditions:
a. Create a map of your LC system (tubing and electrical connections).
b. Keep a daily log.
c. Run test chromatograms regularly.
Step b points out the importance of keeping track of system parameters
and the results of your chromatography during normal operation.
Troubleshooting is easier if you know the typical conditions when the
system is operating correctly.
For example, if your system usually runs at n psi with a certain method,
is the system pressure currently in the same range, or drastically higher
Troubleshooting 3-19
Error messages
Error messages related to the e-SAT/IN module will appear in the Empower
Message Center Log. Click the Message Center icon in the taskbar to access
the log.
Error messages
Troubleshooting 3-21
3-22 Using the e-SAT/IN Module
A Safety Advisories
Contents
Topic Page
Warning symbols A-2
Caution symbol A-5
Warnings that apply to all Waters instruments A-6
Electrical and handling symbols A-11
A-1
Warning symbols
Warning symbols alert you to the risk of death, injury, or seriously adverse
physiological reactions associated with an instrument’s use or misuse. Heed
all warnings when you install, repair, and operate Waters instruments.
Waters assumes no liability for the failure of those who install, repair, or
operate its instruments to comply with any safety precaution.
Specific warnings
The following warnings can appear in the user manuals of particular
instruments and on labels affixed to them or their component parts.
Burst warning
This warning applies to Waters instruments fitted with nonmetallic tubing.
This warning applies to certain instruments when they are in Operate mode.
Caution symbol
The caution symbol signifies that an instrument’s use or misuse can damage
the instrument or compromise a sample’s integrity. The following symbol and
its associated statement are typical of the kind that alert you to the risk of
damaging the instrument or sample.
Important: Toute modification sur cette unité n’ayant pas été expressément
approuvée par l’autorité responsable de la conformité à la réglementation peut
annuler le droit de l’utilisateur à exploiter l’équipement.
注意:未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作該設
備的權利。
注意:未经有关法规认证部门明确允许对本设备进行的改变或改装,可能会使使用者丧失操
作该设备的合法性。
注意:規制機関から明確な承認を受けずに本装置の変更や改造を行うと、本装置のユー
ザーとしての承認が無効になる可能性があります。
警告:當在有壓力的情況下使用聚合物管線時,小心注意以下幾點。
• 當接近有壓力的聚合物管線時一定要戴防護眼鏡。
• 熄滅附近所有的火焰。
• 不要使用已經被壓癟或嚴重彎曲管線。
• 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸。
• 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹,大大降低管線的耐壓能力。
警告:圧力のかかったポリマーチューブを扱うときは、注意してください。
• 加圧されたポリマーチューブの付近では、必ず保護メガネを着用してください。
• 近くにある火を消してください。
• 著しく変形した、または折れ曲がったチューブは使用しないでください。
• 非金属チューブには、テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流
さないでください。
• 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合が
あり、その場合、チューブは極めて低い圧力で破裂します。
Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une
façon non spécifiée par le fabricant, la protection assurée par le matériel risque
d’être défectueuses.
警告:使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用,那麼該設備所提供
的保護將被消弱。
警告:使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用,那么该设备所提供
的保护将被削弱。
警告: ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供
している保証が無効になる可能性があることに注意して下さい。
Electrical symbols
These can appear in instrument user manuals and on the instrument’s front
or rear panels.
Electrical power on
Standby
Direct current
Alternating current
Fuse
Keep upright!
Keep dry!
Fragile!
Use no hooks!
Physical specifications
Item Specification
Dimensions Height = 1.25 inches (3.18 cm)
Depth = 6.25 inches (15.9 cm)
Width = 5.25 inches (13.3 cm)
Weight 530 grams (15.1 ounces)
Power switch Front panel; pushbutton, On/Off
Power indicator Green LED (constant when power is on)
Status indicator Amber LED
Performance specifications
Item Specification
Analytical input range −0.25 to 2.25 Volts
Total input range (no damage) +/− 40.0 Volts (continuous)
Common mode voltage range +/− 3.0 Volts
Common mode rejection ratio 70 dB minimum
(60 Hz)
Input bandwidth 100 Hz
Power consumption 750 mW (typical)
B-1
Performance specifications (Continued)
Item Specification
Voltage accuracy Output within 1% of input
Impedance (common mode) 500 K ohms, +/− 1%, minimum
Impedance (differential) 2 M ohm, +/− 1%, minimum
Nonlinearity 0.01% of full scale
Precision +/− 0.1% for inputs greater than 0.5 mV
Difference in output +/− 1% maximum
Unit-to-unit reproducibility +/− 1% difference in outputs
Buffer capacity 225 Kb
Operating temperature range 4 to 40 °C (39.2 to 104 °F)
Operating humidity range 10 to 90%, noncondensing
Storage temperature range −40 to 70 °C (−40 to 158 °F)
Storage humidity range 0 to 100%, noncondensing
Vibration and shock Negligible
Item Specification
Data format Input = 8 bits, parallel
B-2 Specifications
BCD performance specifications (Continued)
Item Specification
Electrical signal TTL compatible, all 8 bits
characteristics • VIH = 2.0 Volts, maximum
• VIL = 0.8 Volts, minimum
• IIH = 20 µA, maximum
• II = 0.1 mA maximum
• IIL = − 0.1 mA typical (current out of module
shown as negative)
Maximum input voltage +/− 7 Volts
Flow control None
Item Specification
Input specifications AC input voltage range = 90 to 264 Vac
AC input frequency range = 47 to 63 Hz
Input current = .38 A at 115 Vac, .15 A at 230 Vac
Output Output voltage = 5 Vdc at 4 A
specifications Output power = 22 to 24 Watts (typical)
Overvoltage protection = Built in
Physical Height = 3.0 inches (7.6 cm)
specifications Depth = 5.0 inches (12.7 cm)
Width = 1.0 inch (2.5 cm)
Communications specifications
Item Specification
Communications interface Ethernet 10 Base-T
B-3
B-4 Specifications
C Spare Parts
This appendix lists recommended spare parts for the e-SAT/IN module. The
e-SAT/IN module and power supply contain no internal user-serviceable
parts. The e-SAT/IN module and a replacement is offered as an exchange
stand-alone unit only.
Spare parts
C-1
Spare parts (Continued)
Index-1
H O
handling symbols A-12 overview
BCD 1-5
I e-SAT/IN module 1-1
initial state 3-11
installation P
electrical 2-11 parts, spare C-1
electrical requirements 2-2 performance specifications B-1
event cables 2-8 physical specifications B-1
network guidelines 2-4 power supply 1-7
network interface card 2-2 cable 2-12
installation site selection 2-2 connecting 2-11
Instrument Method Editor 3-5 specifications B-3
integrating e-SAT/IN module to powering on the e-SAT/IN module 3-2
busSAT/IN system 2-6 power-on problems 3-18
intended use v power-on self-tests 1-6, 3-2
interfaces Pro interface 3-4
Empower Pro 3-3 purpose and audience iv
Empower QuickStart 3-12
IP address sequence 1-6 Q
ISM classification v QuickStart interface 3-12
L R
LED indicators 3-17 relay loop mode 3-11
local area network 1-2 relay operating mode 3-11
M S
mass spectrometer shock hazard A-4 safety advisories A-1
modular coupler 2-3 sampling rates 1-3
multiple e-SAT/IN module connections scale factor field 3-9
2-4 selecting
installation site 2-2
N sampling rates 3-7
network installation guidelines 2-4 self-tests 3-2
network interface card, installation 2-2 setting
noise BCD preferences 3-8
baseline 1-3 initial state 3-11
smoothing 3-15 relay operating mode 3-11
noise reduction and increased accuracy scale factor 3-9
1-3 units 3-9
Index-2
single e-SAT/IN module connection 2-3
site selection, installation 2-2
smoothing noise 3-15
spare parts C-1 I
specifications
analog channel performance B-2
BCD performance B-2
communication B-3
performance B-1
physical B-1
power supply B-3
summary of LED indicators 3-17
switch, Ethernet 2-4
symbols
caution A-5
electrical A-11
handling A-12
warning A-2
system troubleshooting 3-18
T
TCP/IP setting 2-3
tests, self 3-2
troubleshooting 3-17
error messages 3-20
power-on problems 3-18
system 3-18
U
units field 3-9
W
warning symbols A-2, A-6
Waters Technical Service, contacting
3-19
Index-3
Index-4