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ING. DISEÑO MOLECULAR DE MATERIALES

UNIVERSIDAD DE GUADALAJARA
CENTRO UNIVERSITARIO DE LOS VALLES

APTAMERS DNA

BIONANOTECHNOLOGY
ROGELIO RODRIGUEZ RODRIGUEZ

JOCELIN GEORGINA CASTAÑEDA RUVALCABA

Introduction
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Which are relatively short single-stranded (ss)

DNA molecules comprising 40–120 nucleotides.
DNA aptamers can form unique secondary and
tertiary structures that can bind specific
targets.

Aptamers are single-stranded, non-coding

oligonucleotides (DNA or RNA) that have evolved
through the Systematic Evolution of Ligands by
Exponential Enrichment (SELEX).
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STRUCTURES

Aptamers recognize and bind targets by a 3-dimensional structure


Broadly neutralizing aptamers of SARS-CoV-2: a
diverse panel of agents modified DNA antivirals
Modified aptamers were produced by
conventional solid phase oligonucleotide
synthesis using the phosphoramidite method
The aptamers were synthesized at the 50-nmol
scale in a plate-based system on a Mermade
192X DNA synthesizer with some adjustments to
the protocol to account for the unique base
modifications contained therein.
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SYNTHESIS
Synthesis of modified aptamers
Detritylation was accomplished with 10%
dichloroacetic acid in toluene; coupling was achieved
with 0.1 M phosphoramidites in straight acetonitrile or
a mix of acetonitrile:dichloromethane activated by 5-
benzylmercaptotetrazole and was allowed to react
three times; capping and oxidation were performed
according to instrument vendor recommendations.
The resulting oligonucleotides were deprotected and
cleaved from the controlled pore glass (CPG) by placing
the plate containing synthesis columns in a reactor and
incubating them with gaseous methylamine using an
optimized time, pressure, and temperature.
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We present a novel method for the efficient capture and elution-free detection of DNA using a
CRISPR/Cas9-mediated light-up aptamer transcription (CLAT) assay in combination with a
DNA- capturing pDMAMS-coated tube.

ADVANTAGES

The cleaved DNA-induced polymer-

zation and transcription allows for rapid
assay without temperature changes.

The inclusion of a light-up aptamer

suppresses background fluorescence
and rapidly enhances signals of interest.
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Sensing ability of CLAT assay
with pDMAMS-coated tube
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Diagnosis of tumor
bearing mice using
CLAT assay with
pDMAMS- coated
tube
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Application of aptamer in breast cancer

It is known that aptamer-nanoparticle
(Apt-NP) conjugates are one of the
most valuable systems for cancer
diagnosis. Using these conjugates,
complex body fluids, such as blood
and serum can be analyzed for cancer
cells.

Using aptamers cancer cells re

recognized with high sensitivity and
selectivity, and their nuclease activity is
The method has been widely applied for aptamer selection in vitro. Aptamers are combined with protected by nanoparticles
nanoparticles or imaging labels to target tumor cells for the diagnosis and treatment of breast cancer.
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Aptamers as therapeutic for targeted drug delivery.

Classified into six groups

and ten types

siRNA

LNA

miRNAs
ApDCs

PDT

PTT
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APPLICATIONS
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ADVANTAGES DISADVANTAGES
It is toxic
Is the simplicity of the DNA
purification process.
non-specific

Additionally, our method enables is not biologically accessible

elution-free DNA amplifi- cation and
detection. water insoluble

fast-clearing
It has the potential to enable rapid
DNA identification in complex
biological samples. has multi-drug resistance,
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Referencias

Kang, J. S.-M.-K. (2022). Elution-free DNA detection using CRISPR/Cas9-mediated light-up aptamer
transcription: Toward all-in-one DNA purification and detection tube. Korea:
https://reader.elsevier.com/reader/sd/pii/S0956566323000271?
token=A1AC2633053D376CDA0DAF82D1382190E954BD1C57EF30C1670448AC9EC0346A1857273C11
96460B06C29762FD23D578&originRegion=us-east-1&originCreation=20230312210155.

Rui Fan, X. T. (2023). Application of aptamer drug delivery system in breast cancer therapy. China :
file:///C:/Users/jocel/Downloads/1-s2.0-S0753332223002329-main.en.es.pdf.