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    Gustavo D.B.
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    This document describes a method for separating five penicillin antibiotics using high performance liquid chromatography with a reverse phase column. An isocratic eluent of 50:50 acetonitrile and water with 0.1% trifluoroacetic acid was used at a flow rate of 1.8 ml/min and temperature of 25 degrees C to separate ampicillin, penicillin G, penicillin V, nafcillin, and dicloxacillin, detecting the separated substances at 254 nm.

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    This document describes a method for separating five penicillin antibiotics using high performance liquid chromatography with a reverse phase column. An isocratic eluent of 50:50 acetonitrile and water with 0.1% trifluoroacetic acid was used at a flow rate of 1.8 ml/min and temperature of 25 degrees C to separate ampicillin, penicillin G, penicillin V, nafcillin, and dicloxacillin, detecting the separated substances at 254 nm.

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    © All Rights Reserved
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    1 views1 page

    vph0026j Short Application

    Uploaded by

    Gustavo D.B.
    This document describes a method for separating five penicillin antibiotics using high performance liquid chromatography with a reverse phase column. An isocratic eluent of 50:50 acetonitrile and water with 0.1% trifluoroacetic acid was used at a flow rate of 1.8 ml/min and temperature of 25 degrees C to separate ampicillin, penicillin G, penicillin V, nafcillin, and dicloxacillin, detecting the separated substances at 254 nm.

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    of 1

    Separation of Penicillin Antibiotics

    Method VPH0026J
    HPLC RP Mode

    Column: Eurospher 100-5 C18, 250 x 4.0 mm ID Order No. 25DE181ESJ

    Phase: Eurospher 100-5 C18

    Conditions: Eluent: Acetonitrile / Water (0.1% TFA) (50:50)


    Gradient: isocratic
    Flow rate: 1.8 ml/min
    Temperature: 25 °C
    Volume: 1 µl

    Detection: UV at 254 nm

    Substances: Ampicillin, Penicillin G, Penicillin V, Nafcillin, Dicloxacillin

    Keywords: Drugs, Antibiotics, Penicillines

    Chromatogram: 1
    1. Ampicillin 4
    2. Penicillin G
    3. Penicillin V 3
    4. Nafcillin
    5. Dicloxacillin

    2
    5

    0 5 10 min

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