Videos to binge watch before INICET

NOV 23 RR- PREPLADDER APP

PATHOLOGY ( in order of importance)

5
NEETPG marathon – 2 sessions
2 Pat
One liner session
day
the
foot 15 12 noon
0H t2nod
1
IN
2 day plan for PATHOLOGY LRR
DAY 1
OCTOBER 15TH @12 NOON
• IMPORTANT IHC MARKERS with CK7 CK20 PROFILE
• NK CELLS
• IMMUNODEFICIENCY DISORDERS
• AMYLOIDOSIS DIAGNOSIS
• HISTOPATHOLOGY PROCESSING
• IMPORTANT HISTOPATHOLOGY STAINS
• PROTEIN ELECTROPHORESIS
• PCR TECHNIQUES AND TYPES
• SOFT TISSUE TUMOR TRANSLOCATIONS
• RENAL DISEASE DEPOSITIONS
• MISCELLANEOUS QUESTIONS
2 day plan for PATHOLOGY LRR
DAY 2
OCTOBER 17TH @12 NOON
• CD MARKERS TABLE
• APPLICATION OF MARKERS IN FLOW CYTOMETRY
• FLOW CYTOMETRY BASICS
• IMMUNOFLURESCENCE PHOTOS QUICK WALK THROUGH
• PAP SMEAR IMAGE BASED QUESTIONS QUICK WALK THROUGH
• BREAST CANCER GENETICS UPDATES AND REVISION
• LYMPHOMA GENETICS AND APPLICATION IN QUESTIONS
• RBC SHAPES AND INCLUSION QUICK WALK THROUGH
• IRON PROFILE questions
• LEUKEMIA STAINS (INICET FAVORITE)
• VACUTAINERS AND NEEDLES
• MISCELLANEOUS
Q)70 year old caucasian lady presents with rapidly growing red-violaceous nodule
as shown below. On histopathological assessment, small round blue cell tumor
with high N:C ratio, round / oval nuclei, finely dispersed chromatin (salt and

E
pepper), indistinct nucleoli and scant cytoplasm is noted. Electron microscopic
image of the tumor cells is also shown below. On immunohistochemistry the tumor
cells are positive for chromogranin and synaptophysin. What is the expected
CK7/CK20 profile of this patient ?

A. CK7 + CK 20 +
0800511mn
O
B. CK7 - CK 20 -
C. CK7 + CK 20 -
D. CK7 - CK 20 +

0
asi.IE JmYEcme
 O
Site Name of NET
Thyroid Ca
Medullary thyroid
Lungs
CARCINOID TX

I
Neuro
secretory
granules
GIT
Adrenal
Small cell
CARCINOID TX
carcinoma

Pheochromocytoma
Skin MERKEL cell Ca
8
SALT PEPPER
 H ve
BIT
74 20 Tabone colon

20 CM 20

I ccc
 Eytokeratin
BOTH E
ve 500
I
Bladder DO
CM ZO

RCC
H
7 7
1 Above Sgu
Smcc
colon
Q) 10 year old AFRICAN child presents with a jaw swelling which shows the
presence of a highly proliferative tumor. Scattered amidst the tumor cells are

E
the tingible body macrophages. Which of the following markers is used for the
proliferative index of this tumor as shown below?
A. CD19
B. CD20 BROWN
C. CD23
Prot marker
D. MIB1
M1Bykigy
MIC2 CD99
BURKITT'S
Ewing's Thoo
hymperome sarcoma
99 Gram
 IHC

MINIX
MBH
O
Ki 67
KED N 1001

AFFIANT
Bv endemic fatty Prot marked

starry 518,513,5g

08 OTy Tumor
cells

appearance
8
BURKITT's
lymphomac
myo gene
tf even mo

22
14
2
Q. Which of the following Her2neu immunohistochemical studies will be
further evaluated using FISH?

0 jBRESTca
negative
Herznen IHC

2 i

O O o Ofositive
t
I
Krastos
 Bo Ca

BROWN
 if
HER 2 NEU a
17
D green
Her Herz red
He
It
52
2.5

72
 Q) 3 year old presents with a vaginal mass as shown below
(image1). Biopsy and histopathological assessment from the same
reveal a tumor (image2). Which of the following
immunohistochemicalÉT markers will aid in the diagnosis of the
condition

a. vimentin, desmin 8
b. cytokeratin, desmin
c. CD99
d. Vimentin, SMA

spgstaying
my
hospho Tungstic Acid Hem at
 E M

GO
P
E
RMS LCH GIARDIA C.DE Ium

I Tennis
Brubeck Racket
granules of
Langerhans cell
 BROWN chromogen

YO FRED AEC

I IMMUNOHISTOCHEMISTRY
CHROMOGEN
DAB
BENZIDINE
DISEASE MARKER

CARCINOMA

SARCOMA

LYMPHOMA

MELANOMA

LCH

LEIOMYOSARCOMA

RHABDOMYOSARCOMA
LUNG CARCINOMA MARKER

SQUAMOUS CELL
CARCINOMA
ADENOCARCINOMA

SMALL CELL CARCINOMA

MESOTHELIOMA
SERATIN PEARLS
GLANDS
SALT PEPPER AZZOPARDI EFFECT
 D
I
Q) 29 year old male patient presents with a red plethoric face along
with episodes of dizziness. The patient is found to be hypertensive on
examination. Further he also gives history of intense burning
sensation in hands and feet along with itching following a hot water
bath. On CBC analysis his Hb is 21gm/dl. He is advised EPO level
testing along with JAK2 testing. EPO levels are found to be
suboptimal. JAK2V617F mutation is found in this patient. Which of the
following methodology of testing must been used for the testing of the
same
1.Sanger sequencing
2.SBPE
3.qPCR
4.GWAS
Sanger sequencing

SBPE- single base primer extension

Pyrosequencing

Amplicon length analysis

qPCR quantitative PCR/ Real time PCR

Reverse transcriptase PCR

GWAS- genome wide association study

Sanger sequencing

SBPE- single base primer extension

Pyrosequencing

Amplicon length analysis

qPCR quantitative PCR/ Real time PCR

Reverse transcriptase PCR

GWAS- genome wide association study

Q) 15 year old male presents with a typical facial profile of long face,
large mandible and large years. Mother gives history of dropping out
of school owing to extremely poor grades. On physical examination,
testes are enlarged. Genetic testing is advised on the basis of a
similar condition reported in one of the distant cousin. The following
finding is noted in the X chromosome when grown the cells are
grown on a folate deficient medium. Which of the PCR methodology
is best suited For the diagnosis of this disorder?
1.Sanger sequencing
2.Amplicon length analysis
3.pyrosequencing
4.GWAS
Q) NARP syndrome is a ?
A. Lysosomal storage disorder
B. Mitochondrial function disorder
C. Glycogen storage disorder
D. Golgi body transport disorder
• MELAS

• CPEO

• LHON

• LEIGH’S SYNDROME

• PEARSON SYNDROME

• KEARN’S SAYRE SYNDROME

MERRF
What is the type of inheritance where an affected male does not
have affected children, but an affected female always has
affected children?
a. X linked recessive
b.Autosomal recessive
c. X linked dominant
d.Mitochondrial
Q) Which of the following is false with respect to amyloid
1) has properties of starch
2) beta pleated sheet configuration on electron microscopy
3) diameter 7.5-10nm on electron microscopy
4) stains salmon pink with Congo red on light microscopy

Options
A) 1,4
B) 1,3,4
C) 1 only
D) 2 only
Is

WAXY
kidney
AMYLOID
Which of the following statements is true concerning amyloidosis?
a. SAA forms amyloid in multiple myeloma
b. On Congo red staining, amyloid shows apple green birefringence
c. In AL type amyloid is most commonly formed in acute inflammations
d. β2 - macroglobulin forms amyloid in systemic senile amyloidosis
Q. NK cells attack which of the following cells?

a. Cells which express MHC I

b. Cells which are not able to express MHC I
c. Cells which express MHC II
d. Cells which are not able to express MHC II
Q. BAFF genetic defect associated with germinal centre
hyperplasia leading to hyperplasia of peyer's patches and
tonsils?

a. Bruton’s agammaglobulinemia
b. SCID
c. CVID
d. CGD
Q. Select the incorrect match?

a. Del 22p - DiGeorge syndrome

b. Baff - CVID
c. ADA deficiency - SCID
d. BTK – Bruton’s hypogammaglobulinemia
Disease Defect Clinical
Severe combined
immunodeficiency

Bruton’s hypo-
gammaglobulinema
Disease Defect Clinical
Bare lymphocyte Sx

Di George syndrome
BRUTON’S V/S CVID
Disease Defect Clinical
Hyper IgM syndrome

Hyper IgE syndrome

Disease Defect Clinical
Wiskott Aldrich
syndrome
Q. A male patient presents with eczematous rash over the
body along with repeated bleeding episodes. Patient also
gives history of repeated infected. On peripheral smear
O
examination patient is found to have small
EE sized
platelets. Diagnosis?

a. CVID
b. CGD
c.
d.
WAS
Ae
DiGeorge syndrome

Bernard

Bigity Soter
I TP
A 27 year old woman presented with multiple bilateral cervical that were gradually
increasing in size over 6 months. On examination multiple lymph nodes are
palpable in the cervical region with largest measuring 5.5cm. Similar multiple
nodules are noted on both arms as well. FNAC is performed from the largest
lymph node and shows the image attached below. Based on the morphology,
which of the following is the most likely diagnosis?

A. Tubercular etiology
B. LCH
C. Reactive process
D. Rosai Dorfman Disease
Q. Which of the following disease is associated with storage of
nonfunctional protein in hepatocytes?

A. Alpha 1 antitrypsin deficiency

B. Tay-Sachs disease
C. Familial hypercholesterolemia
D. Wilson disease
Q. Liver biopsy of a 4 year old boy reveals
intracytoplasmic inclusion on liver biopsy
with PAS stain. Which of the following
serum protein electrophoresis graph
will you correlate with this condition?
Arrange the following steps in the correct sequence of histopathology
processing?

1. fixation
2. Clearing
3. Mounting
4. dehydration
a. 1,2,3,4
b. 1,3,2,4
c. 1,4,2,3
d. 1,4,3,2
TISSUE PROCESSING
• Fixation
• Dehydration
• Clearing
• Impregnation
• Embedding and blocking
• Section cutting
• Routine staining
• Mounting of slide
Q) Find the correct match?
• 1) t(17:22)
A) ASPS
• 2) t(9:22) B) Extraskeletal myxoid chondrosarcoma
• 3) t(12:15) C) DFSP
• 4) t(x;17) D) Infantile fibrosarcoma

A) 1-A,2-B,C-3,D-4
B) 1-C,2-A,3-B,4-D
C) 1-C,2-A,3-D,4-B
D) 1-C,2-B,3-D,4-A
Q)INFANTILE FIBROSARCOMA is associated with?

• A) t(11;22)
• B) t(12;15)
• C) t(2;13)
• D) (12;14)