Stock

S.N. Reagent Quantity

concentration
Nuclease Free
1 1.5 µl
Water
2 Mastermix 1x 5.0 µl
3 Forward primer 100pmol 0.75 µl
4 Reverse primer 100pmol 0.75 µl
5 DNA template 150ng/µl 2 µl
Total volume 10 µl
 Temperature
S.N. Steps Duration
(ºC)
Initial
1 95 4min
denaturation
2 Denaturation 95 40sec
3 Annealing 50-61 33sec
Initial
4 72 40sec
elongation
5 Elongation 72 5min
6 Hold 4 ∞
Number of cycles 31