$1000" Thermal mu te ___ Quick Guide Amitionion|Main Menu and Controls Use the rain menu to access the thermal eycler’s major functions: RUN protocol, creata a NEW protocol, EDIT a protocol, VIEW a protocol, select FILES, ‘or access TOOLS to change the thermal cycler settings, Use the keys on the instrument's control panel forthe functions shown below, ece , ao eco 2 <@> eco *°" | ece "$1000" Thermal Cycler SOREEN— tocelay auto Sasor case Sree rat Dawn eencaland §——NCLEATE —toinstanty CANCEL —tocarnat TI SEOT test pronsol ous ‘Jacianinoubeton wen snacton oretumta —Upand down aon — Hhiniohoctmeatery — Meprevcusscreen —iwenterleiers temperate = ENTER — to elect the highlighted cemmand Use the marimenu to aceess major functionsRunning a Protocol 4. Select AUN in the main menu; ppross ENTER, 2. Selecta protocol press ENTER. S.Edit the volume, if desired; press ENTER, 4, Select FUN; press ENTER, Creating a Protocol 1. Select NEW in the main menu; press ENTER, 2, Ener a name and sample volume: press ENTER 8. For the intial stop, select TEMP for a ‘temperature step or GRAD for a gracient step and enter values; press ENTER, 4. For additional steps, select TEMP, GRAD, of GOTO and enter values; press ENTER, | 5.To complete the protacol, select END; | press ENTER, 6. Salect a folder and save the protocal, Eaiting a Protocol 1. Select EDIT in the main menu; press ENTER. 2. Select a protocol, press ENTER. ‘3. the sample volume andlor id temperature, f desired. 4, Use the arrows to highlight and change value. 5.To insert or delete a stop, highlight the step number and select INS or DEL. (6.Select END; press ENTER to finish; changes are saved to the fle you selected, Erte stops Croat eee Choose anew sep typ (Choose an aonOpening and Closing the Heated Lid Opening the Lid eo 1. Tum the knob counterclockwise to raige the inner lid 2, Raise the handle up to the vertical position, Glosing the Lid 4. Using the handla, push the fd down, 2. Lower the handle to the horizontal position Until the lid latches securely (see inset. 8, Turn the knob clockwise until the: inner lid contacts the vessels, then ‘add one-halt tur for sealing tape or ‘one-quarter turn for caps, {nas US Pa Nr Sars ofeure 18 YOO ard Ty, ceapondngcle ‘recta lerarg, opi Boone, 050 neon cate bv Post xy, cons e230 USK “ppt carrey anor ch ong U.S tn or tha oi Exeansnt as Pat ese 7832 ms TRE esnips ce by © SP aa Life Science ete ou SER Ean SIEY sora, Bias ce titats ie tomcat ee me ee acca eae Smee Sith orm Ee suite an emote ronerearevA USI to-e1s ostt scart