BIOTECHNOLOGY AND ITS APPLICATIONS

1.A multinational company outside India tried to sell new varieties of turmeric without proper patent rights. What is such
an act referred to?
2.Suggest any two techniques which can help in early detection of bacterial / viral infections much before the symptoms
appears in the body.

3.How does silencing of specific mRNA in RNA interference prevent parasitic infestation?

4.Name any two techniques that serve the purpose of early diagnosis of some bacterial /viral human diseases.

5.What is genetically modified food? What are the dis-advantages of this food?

6.Write full form of ELISA. Give an example of the clinical application of ‘ELISA’ test.

7.Describe the responsibility of GEAC, set up by the Indian Government.

8.Nematode specific genes are introduced into the tobacoo plants using Agrobacterium vectors to develop resistance in
tobacco plants against nematodes. Explain the events that occur in tobacco plant to develop resistance.

9.How is a transgenic tobacco plant protected against Meloidogyne incognita? Explain the procedure.
10.Expand the name of enzyme ADA. Why is the enzyme essential in the human body? Suggest a gene therapy for its
deficiency.
11.Plasmid is a boon to biotechnology. Justify this statement quoting the production of human insulin as an example.

12.Highlight any four advantages of genetically modified organisms (GMOs).

13.How did EI Lilly company go about preparing the human insulin? How is the insulin thus produced different from that
produced by the functional human insulin gene?
14.Why is introduction of genetically engineered lymphocytes into an ADA deficiency patients not a permanent cure?
Suggest a possible permanent cure.

15.How does RNA interference help in developing resistance in Tobacco plant agains nematode infection.
16.How did Eli Lilly synthesize the humun insulin? Mention one difference between this insulin and the one produced by
the human pancreas.
17.Name the insect pest that is killed by the product of cry I Ac gene. Explain how the gene makes the plant resistant to the
insect pest.
18.Why do the toxic insecticidal proteins secreted by Bacillus thurigniensis kill the insect and not the bacteria?
19.Name the fist transgenic cow developed and explain the improvement in the quality of the product produced by it.

20.Explain the process of RNA interference.

21.Explain how a hereditary disease can be corrected. Give an example of first successful attempt made towards correction
of such diseases.

22.How is “Rosie” considered different from a normal cow? Explain.

23.Biopiracy should be prevented. State why and how.
24.What happens when Meloidogyneingonnita consumes cells with RNAi gene?