Self-Assessment of Chapter 5 1. Reversible Ligand Binding Three membrane receptor proteins bind tightly to a hormone. Based on the data in the table below, (a) what is the Kd for hormone binding by protein 2? (Include appropriate units.) (b) Which of these proteins binds mast tightly to this hormone? | Hormone | concentration (nM) 02 0.29 0.17 oxy 05< (0.51 0.33 fea1< 0.67 0.5) | a 0.89 0.8 } to 0.95 0.91 } 20 0.97 0.95 | 50 0.99 0.98 © by olssrvetieon Oras befall Kye tum, kd, = osnm kd = fonm , a i ulation ——— 1-9) orgy by eale lation 6 iat) Kes ae Me , v exert, va 2 IO eins 4am Or kirce protaing 46k, [east g 2 tlvee prtsios vas cogent i te leas ctleve preteona Vinds mast 1% te tly em ples hefauond 2. Reversible Ligand Binding The protein calcineurin binds to the protein calmodulin with an association rate of 8.9 x 10° M''s™ and an overall dissociation constant, Kd, of 10 nM. Calculate the dissociation rate, kd, including appropriate units, haz §.2rie M's" Gla nwterivé + Gate odalin a ——* Gl: neil kgs roe zo x10 _ ka “ Re Te ka: Ka = Be - Se = 1x1 Mm x b4ero MS) = 2axIO S |3. Hemoglobin’s Affinity for Oxygen What is the effect of the following changes on the O2 affinity of hemoglobin? (a) A drop in the pH of blood plasma from 7.4 to 7.2. (b) A decrease in the partial pressure of CO? in the lungs from 6 kPa (holding one’s breath) to 2 kPa (normal). (¢) An increase in the BPG level from 5 mM (normal altitudes) to 8 mM (high altitudes). (@) An increase in CO from 1.0 parts per million (ppm) in a normal indoor atmosphere to 30 ppm in a home that has a malfunctioning or leaking fumace. tof onesie bee declenes. a Plew4 te p2 O, effin > Pro, decronr. Oo. - oe. \ if BPG imcyene tleerese lecteme do CO weyenre decter 4, Reversible (but Tight) Binding to an Antibody An antibody binds to an antigen with a Kd of 5x10 M, At what concentration of antigen will O be (a) 0.2, (b) 0.5, (€) 0.6, (d) 0.8? On? t ; oka = L)) = =a ie Os Fags + SCM TED = WEG ka = setoemM ' Ovr% Serio Mm ob = we = h2sx1oM O= 02,0) —— 2, oS vseotM . gras UT po = svt +8 >. ergo’ OR | 6rd6 (ls gee. Toe ER OK IOS, 620% tLTe ous M0? or Sole “peerion a gn get bs for tack Oe ree FLT osat 1M 5. Relationship between Affinity and Dissociation Constant Protein A has a binding site for ligand X with a Kd of 10° M. Protein B has a binding site for ligand X with a Kd of 10_9 M! Which protein has ‘higher affinity for ligand X? Explain your reasoning. Convert the Kd to Ka for both proteins. AtX > AX Kel 10" 4 Btx 9 BX Kazio tM oe Ka fot prwtrin x Corespler is Salley phan ebet of P =X corp, wage Pfoern B—X Are more yerble thon Proesmls ae hig affinirg fot ligand B ' Ka = ar \ ’ ~ poo AX coop Danae = 7g4,= 10 rete [De ds l a — a Kae es a= OTM 6. Draw a sketch of the structure of atypical antibody and describe the structure Indicate each subunit in in detail as much as you can. Seeit ~ gon text book