Professional Documents
Culture Documents
Locate the red-brown liver in the upper central abdominal region. The rabbit liver comprises the
right, left lateral, left medial, quadrate, and caudate lobes. Identify the left lateral lobe, lying
infero-lateral to the left medial lobe. Now, draw out the left lateral lobe and place it onto a dry
gauze to prevent retraction into the abdominal cavity. Cover the organ with a wet gauze to keep
it hydrated.
Meanwhile, process the tumor tissue of interest - obtained from a tumor-bearing donor - to
acquire appropriately sized fragments. Using a blade, puncture the exteriorized liver capsule deep
enough to create a small pocket for tumor fragment implantation.
Transfer the processed tumor fragment inside the pocket and remove the blade. Apply a
hemostatic agent over the pocket to stop bleeding and prevent ejection of the tumor fragment.
Post-hemostasis, return the liver inside the abdominal cavity. Suture the surgical incision and
allow the rabbit to recover. Monitor the animal weekly, observing the growth of hepatic tumor.