https://pubs.acs.org/doi/pdf/10.1021/acs.langmuir.

9b02652
13 nM nanocząstki złota z TECEPem i dołączanie nukleozydów/DNA:

For SCN−, GSH, and

TCEP, they destabilized the AuNPs at high concentrations
(2−10 mM). The GSH and TCEP samples
fully returned to red and thus their aggregation was reversible

The TCEP-capped AuNPs showed

a diffused red band with a bit of trailing, which also indicated
that TCEP had a moderate affinity for the AuNPs

Interestingly, the TCEP-treated samples all remained red

regardless of the added nucleoside (Figure 5A, bottom row).
Figure 5. (A) Photographs of citrate-AuNPs (top row) and 1 mM TCEP-treated AuNPs
(bottom row) incubated with various nucleosides (1 mM)
in HEPES buffer (10 mM, pH 7.4). (B) Adsorption of FAM-labeled, nonthiolated A5,
T5, or C5 DNA onto citrate- or TCEP-capped AuNPs.
Excess TCEP was removed before adding DNA, and no NaCl was added. (C) Effect of
free TCEP (1 mM) on the adsorption of FAM-labeled
DNA onto citrate-capped AuNPs. (D) Effect of free TCEP on the adsorption of two
thiolated DNAs (FAM-9T5-SH and FAM-9A5-SH). No extra
NaCl was added in these experiments. (E) A5 DNA adsorption on AuNPs in 10 mM HEPES
or phosphate buffer (PB). (F) Scheme of enhanced
DNA adsorption by TCEP.
Figure 6. (A) ITC traces of titrating TCEP into 20 nM, 13 nm AuNPs. ITC traces of
titrating TCEP into (B) A5 and (C) T5 DNA. The titration
traces (top) and the integrated heat after background subtraction (bottom) of each
sample are shown. The pH of 5 mM TCEP was adjusted to 7.4
and the AuNPs were finally also dispersed in 10 mM HEPES (pH 7.4).
Langmuir Article
DOI: 10.1021/acs.langmuir.9b02652
Langmuir 2019, 35, 13461−13468
13465
Therefore, TCEP protected the AuNPs, suggesting that even
adenosine cannot displace TCEP (i.e., TCEP was adsorbed
more strongly than all of the nucleosides). It should be noted
that when an oligonucleotide is used, its adsorption affinity is
still stronger than TCEP due to the polyvalent effect.55,56 For
example, when we preadsorbed nonthiolated 15-mer DNA on
AuNPs, TCEP can barely desorb them

Supplementary Materials: Unique Properties of Core

Shell Ag@Au Nanoparticles for the Aptasensing of
Bacterial Cells
`
Before immobilization of the
aptamers at the surface of the modified CPE, a mixture of 5 μM of each aptamer was
mixed with
5 mM of TCEP (1:1 v/v) and kept in the dark at room temperature for 1 h. During
this incubation time,
disulfide bonds of aptamers were cleaved and then could bind with gold via a
covalent bond [3–5].
The TCEP solution should be prepared immediately before use. The final
concentration of the
aptamer cocktail on the surface of the working electrode is 2.5 μM.