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Viyeons-n, Gyeongsengnam do Typeated ‘ed beverage — | rodct em manutnciring ep namber | POPTO6OOOTT Product name few hydrogen mira water Shai T2mones Bs before da Raw mater nane or inreien name snd mixing | Shown 0 he bck page ‘aio Purpose andnwio wie | Showa onthe ac page Produ [Howto ster and phase a ae ee 7 Showa an he beck pase a How to package and package | shown on the back page a nit Pho apni Th ve ra ar ie Be POA TONE NE TR Chancerisis afte pomtEn tls this prodet igh ow-nuion ood? WEST No TO] NetApp 1s ths prot led and si or consumption y ifn? [] Ys [0] No 1 sth prot sterienonpascrzton po? ?[] Nt seized [O} Strid] Paseuried (others I | In accordance with Article 37 Paragraph 5 of the Food Sanitation Act and Article 45 Paragraph I of the Enforcement Regulations of the same Act, we report the matters pertinent to the manufacture of the food (food additives). May 18, 2021 Reported by Woo Jik, Lee To: Uiryeong County Govemor, Gyeongsangnam-do Product item report number: 202106090331 Environment and ‘Sanitation Processed by Depaaiment, | Processed by Seung Yong, Lec | Processed date | December 08,2021 Economy and Culture Bureau Document No: MAMB-BMAH-WCLC-RFDH-VPWH (Raw material name or ingredient name and mixing ratio) Na] RaW airal or egredionis name nd | Miniogvatio |, | Raw mail or ingredients mame Mining aio 7 mixing ratio ca. and mixing ratio, real 1 | Underground water 99.99988% 2 | Hydrogen 0.00012% Document No.: MAMB-BMAH-WCLC-RFDH-VPWH Purpose and how to use ‘Purpose: for consumption, to drink How to use: drink as itis How to store and package material ‘Avoid direct sunlight and store in a cool place Polyethylene (interior) How to package and package unit 300ml, 500m! ‘BMS | MAMB-BMAH-WCLC-REDH-VPWH } AS - ASSIt2 SSWAZLDA ae gee wae L228 19684 098) 158) Ba wave | Geel se2 keBee 211 (AITIS) RUSH 1035200472 ERICEd) aessue aes | oaas 20210605003 SHA Ades INE FBO OAD 8 Neo ae euee Benzene [2oevsos RAG HOSE raw geRne SRS Se SFE leew 3a BS 8B S201 1M mags | save g zane [801 10 - Baye x zeea [S01 IM ae HEQRY SAD VOUS HAD O11, O14 BoIOr eC ae Sa Se Nee Ae we OF | Jot | JOLIe [olwe we mAs ERUBSS EN Ly ue mee-ge melas oF [ues (182 | Be Diet "ABM, NGA MS S BS So AMR MASA AIO Ma Vw (NBG Is) SSM5 ASS SDS 20214 058 182 wpe o1eal Bass A+ aa Seenue : 202106090031 navd [adeos eanan| nenes [ose Merexr [aoeet 128 06s! ‘WBS | MAMB-BMAH-WCLC-RFDH-VPWH (AMES C= SES @ wes) et No ane se see “RIB [no.| sige ce tee wee + | mee 99.99988 ae % 0.00012% ‘Was: MAMB-BMAH-WCLC-RFDH-VPWH go: 48. 88 so388 ee: 20e 3A Bae o AMNBES WD MSs RO wat ZaMe Bel ole ae!) Hage @ aes 300m! 500m! ASSN SET FE19, 403-12 (BEVh, Sells Ba oe nreen Oil (Ash 778-6313~4 (42) 771-8189 awage I swear that the attached translation is gud. true to the original. 20228 058 2491 May 24, 2022 yr 9 BOE Signature is SB 20224 Al 8823 Registered No. 2022-8823 Ql é Notarial Certificate 1 8 & WOO CHANG HA personally 2 BS29 Waa) HWE] A — appeared before me, confirmed that ES VAISS Blan AVE the attached translation is jrue to the aiatch original and sub 20224 053 24 ol AHF OIAL 9 1S. This is hereby attested on this 24th day of May 2022 at this office. SHINHAN LAW & NOTARY OFFICE Seoul Central District Prosecutor's Office #403-1, 19, Jong-ro, Jongno-gu, Seoul, Korea l-<.->07 aS. STSASAL BAPBAE UAE w325H0) 7 sha] 20054 018 038 WEF GALS HE} BS UES VE AS U7 wat Signature of the Notary Public KIM MOON SOO This office has been authorized by the Minister of Justice, the Republic of Korea, to act as Notary Public Since 3, Jan. 2005 Under Law b 25. 210mm X 297m BESACB) Ohm Certificate of Authentication ‘Seen atthe Mins of Foregn fair ofthe Repub of Korea ‘Valeriy it submited to een mission nthe Repub of Kora, 1. Country Republi of Koren Certified “Tovey the Certicat, please refertothewetste below. ‘nig poste gokr Pat seou She 24/05/2022 417 The ministry of Foci Aas S.No. xcronvarIsvC 7.Signature Park Kwan Seok J pial lel: ae aay bs aia vl 935 403-1 ® ” vo cree Meme ee (2) 771-8189 Registered No, 2022 - 8824 NOTARIAL CERTIFICATE SHINHAN LAW & NOTARY OFFICE #403-1, 19, Jong-ro , Jongno-gu, Seoul, Korea 210mm X 27mm MEGALB) TOp/at 18 Eaforcement Rules ofthe Groundwater Act [Annex: Form No. 12] Phone (053-622-6198) No.20222 Certificate of Completion of Construction ‘Name (Casiom BCo,tid) | Date of bin 171511-0014408 {Company name) (Resident registration cubs) Repoted by ‘Address (location of the main office, in case of a company) Custom B Co, Ltd, $3, Oso-gil, Burim-myeon, Uiryeong-gun, Gycongsangnam-do Location 80-2, Dacgok-ri, Burim-myeon, Uiryeong-gun, Gycongsan Coordinates Development and use . longitude se) 128"21" 46.74 35" 28" 51.25” Purpose (specific use) | Drinkable/not drinkable ‘Water for industrial use | Drinkable Excavation depth 123m [Excavation diameter 200mm Installed facilities Planned water intake 150m*/day Power unit: 75 HP Inner diameter of discharge pipe 50mm Pumping facilities Installed depth: 90m Pumping capacity 356 m*iday Other details I certify that the above facility has been certified for the completion of construction in accordance with Article 14, Paragraph 3 of the Enforcement Decree of the Act, such as Article 9 of the Underground Water Act. Jan 12, 2022 Uiryeong County Governor Official Seal Affixed Hereon mudee NeRaieR mize we) 2022-2 zz zZ zesxa85 Fa cae eda) Caen BEER 6) rrstt-coresos @ 2 ol lsa(woe aeae Fe Ae AS ANTI) Oo asus Ise WEO OA2 53, (S)HABDI (waretg: 059-622-6198 [ aH Bes (ez Feo HRA soUN 20 mw.org PSY . us [BE@SAS) 25M" BS) 128" 21! 46.74" ee as__26' 51.28" “ j2ezo. 123 m|B& 18 Naat - us jasane 150. m/e 7 75 “plsaaeune som geayl [SRE% * 4S Jeena! 90m] gy see 356 "7/8 ae Ne r THOSE, MOKA VS YAMS MiARMSHO Ge 11 ASO Ges SSRIs Hwee 2 egeuc. a meu og 28 210mm x 297mm {84K 80u/o (Hh SB)! (als) 778-6313~4 (#4) 71-8189 Aer I swear that the attached translation is true to the original. May 24, 2022 Signature A S¥ 20224 Al 8824e Ql eS a past 2 Bess Baa 9 wage a eo adaee daa ABT asic. Registered No. 2022-8824 Notarial Certificate ‘WOO CHANG HA. appeared before me, confirmed that the attached transl4tiqn.is' to the =| original and subseri ) name. personally 20224 05% 24 o] ARF AA A] AY Seep. This is hereby attested on this 24th day of May 2022 at this office. SHINHAN LAW & NOTARY OFFICE Seoul Central District Prosecutor's Office #403-1, 19, Jong-ro, Jongno-gu, Seoul, Korea <3 1S. Signature of the Notary Public KIM MOON SOO This office has been authorized by the Minister of Justice, the Republic of Korea, to act as Notary Public Since 3, Jan. 2005 Under Law No.325. 2lOmam X 297mm BESAC#) T0alnh Certificate of Authentication ‘Sean et the Minty of Fregn Mars ofthe Repu of Korea ‘Vaid ei eubmited to fooign mission nthe Republic of Korea, 1a :Repubct Koa Certified “Tovey the Cacao please eet the website belo, Ips: posite gohr 2at Seoul Site par05/2022 ALY The Ministry of Foreign Affairs S.No. yo1¢2022V96723M * 6Seal/samp TSigpature AE: Park Kwan Seok et tienen oid ‘Aout 57.00 ] 2 Sree ashes os a ALT aeyblighis Masao wl nase «| OF (ash 778-0919-4 #3 ore an (4) 771-8189 Registered No. NOTARIAL CERTIFICATE SHINHAN LAW & NOTARY OFFICE 2022 - 8825 #403-1, 19, Jong-ro , Jongno-gu, Seoul, Korea 210mm X 27mm BEG ACE) T0e/ni 1 Enforcement Rules ofthe Groundwater Act [Annex: Form No, 12], No.2 Certificate of Completion of Construction | Name (Custom BCo,L48) | Date ofbinh 171511-0014408 (Company nam) (Resident egsration number) Reported by Address (location of the main office, in case of a company) Custom B Co,Ltd, $3, 0s0-gl, Burim-myeon, Uiryeong-gun, Gyeongsangnam-do Phone (053-622-6198) Location 79-5, Daegok-ti, Burim-myeon, Uiryeong-gun, Gyeongsangnam-do Development and use jcominnss longitude, latitude) Purpose (specific use) | Drinkable/not drinkable 128" 21°47.32" ‘Water for industrial use | Drinkable 35" 28" 50.84" [Excavation depth 122m —_| Excavation diameter 250mm Installed facilities Planned water intake 4om’/day | Power unit: 7.5 HP Inner diameter of discharge pipe 50mm Pumping facilities Installed depth: 90m Pumping capacity 356 m*/day Other details I certify that the above facility has been certified for the completion of construction in accordance with Article 14, Paragraph 3 of the Enforcement Decree of the Act, such as Article 9 of the Underground Water Act. Jan 12, 2022 Uiryeong County Governor /S/Official Seal Affixed Hereon masse wepulen m2eMel 2000-98 zaps z= zEeeas ae sume tere) ( Gaasel 8288 ve) 1715110014408 waa beeeasseeceeWeSe Bowe ome mae oan 59, F)ASH (wave: 059-622-6199 | | AR - aves ue wee Ua TOT Se wwe 01 Us |REBEAS) SS(N% BS) 12a" a" 47.32 ee = 35°28" 50,84" . - Bexo 122m [28 NB Al Hd 2 [Renee ca we | 7s Helee2oans som esau [S880 US Ve rzot 80 os 2 356 «/2! a 42) Ast FOE, MOZY VS YAMA MiazASeHOl Me} Aoi Asal Ue Sars ees 3 sgeuc mee oe 210mm x 297mm IAN 80g/0' (48 SH) ARH SET SEL, 403-15 Er, BlAe FSH) seo rael Al or (ash 778-6313~4 (4) 771-8189 AMALS AEH AWIISL Yee I swear that the attached translation is gud. true to the original. 20224 058 249 May 24, 2022 aa 2A OF Signature EE 5 2022 Al 825% Registered No. 2022-8825 Ql S Notarial Certificate 9] 938} __________2 WoO CHANG HA personally BVSdq wat 2 appeared before me, confirmed th: EX 3dUSS Bsa ABT the attached translation isytrue:s arith. original and subscribed hii 202214 058 249 This is hereby attested on this o] ARR AAA] 9] See}, 24th day of May 2022 at this office. SEU ATAU A SHINHAN LAW & NOTARY OFFICE Seopa MARRAS Seoul Central District Prosecutor's Office ANSSY) SET FHI, 403-1E #403-1, 19, Jong-ro, Jongno~gu, (S217, 20112 SEL) Seoul, Korea Les tt S. Beyqusy gk = Signature of the Notary Public KIM MOON SOO PAPAL U7hAS 71325E41] 718°] This office has been authorized by the 2005'4 019 039 WEE AY2=E¥=e] — Minister of Justice, the Republic of SEU USS VY AS U7 WI. Korea, to act as Notary Public Since 3, Jan. 2005 Under Law No.325. 210mm X 297mm BESA8) Tel Certificate of Authentication ‘Sanat the Wins of Foruign Afr ofthe Republi of Korea. Vali only submited to freign mission inthe Repub of Korea, 1. County: Republi of Korea Certified ‘over the Getic, please referto the webste elo. hipaa apostle go kr 2at Seoul She 24/05/2022 APY The Ministry of Foreign Affairs S.No. yoic20221035834 6 Seal/stamp 1.Signatore JE: Park Kwan Seok eaten De 2rivate2 Way Amougt: 37.00 KFA Yy Soren ee alg Jol ae Tet #] geemarae ALOT cata r7e-enns-4 u (42) 71-8189 Registered No. 2022 - 8826 NOTARIAL CERTIFICATE SHINHAN LAW & NOTARY OFFICE #403-1, 19, Jong-ro , Jongno-gu, Seoul, Korea 210mm X 297mm MEBANE) 70e/t Korean Journal of Waters 5(2), 25-28 (2016) The Antioxidative Effect of Hydrogen Water Drinking in Blood Eun Sook Jeong, Dong Heui Kim, Kyu Jae Lee Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea (Received September 9, 2016; Revised October 18, 2016; Accepted October 28, 2016) To confirm the antioxidative effect of hydrogen water drinking, the participants were divided into two groups, purified water (PW) drinking group (n=20) and hydrogen water (HW) drinking group (n=20) because of cross test in each 10 in a group. Purified water (pH 6.9, +432mV) was produced only by physical filtering using, ‘commercial carbon filter, and hydrogen water (ew Hydrogen Rich Water™, Hayannamoo Beverage Co. Ltd., Korea) was used in this experiment (pH 7.5, -650mY, hydrogen .Sppm). Fifty ul. of blood was collected before drinking water, at | and 2 minutes after drinking I2mL/kg, ROS in blood was examined using FORT test kit. ROS was showed lower tendency in HW group than PW group after drinking, and especially lowest tendency in ‘a minute (p<0.01) and 5 minutes (p<0.05). But, there is no the antioxidative effect in purifi Consequentially, drinking hydrogen water is useful to remove active oxygen, ROS. Key words : Antioxidation, Drinking, Hydrogen water, ROS, Introduction In modem society, life expectancy is increasing, and various diseases are occurring due to complex social structure and stress, and government-level efforts and medical support to manage them are also on the rise However, he treatment of modern medicine to improve various diseases caused by work stress, modem diet, and social anxiety is showing limitations. In particular, in Korea, which is entering an aging society, the number of elderly patients is rapidly inereasing, and most of the diseases are chronic diseases, which incur high costs. The problem of medical expenses for chronic diseases is emerging as a big problem for families and the country. Ithas been found that active oxygen is the main cause of chronic diseases. Active oxygen is an oxygen compound that is generated not only in the metabolic process of body cells of animals and plants, but also in large amounts by external environmental pollution such as ozone, nitrie oxide, ultraviolet rays, and smoking, These are known to cause aging, arteriosclerosis, dementia, cancer, ete. (Lee et al., 2006). In general, in the normal human respiration process, about 95% of oxygen is used to make energy, and 4-5% is used to produce active oxygen. The generated active oxygen play an important role in the cell signaling system or generation, act as a cell messenger, and regulate oxidation/reduction reactions (Harman, 1956). They promote the activation of enzymes, drug detoxification and glycogen synthesis. If there is an appropriate amount of these, it protects the body from bacteria and foreign substances. On the other hand, when they occur excessively, they attack normal cells indiscriminately, causing chronic diseases, and becoming the main cause of various diseases such as cancer, diabetes, dementia, and aging (Freeman & Crapo, 1982; Cross et al, 1987). More than 90% of modem disease causes are active oxygens, and they have been found to be the cause of aging (Slater, 1984). Hydrogen is being used as a powerful antioxidant that removes active oxygens. Hydrogen is reported to be effective in both drinking and inhalation on the human body, and itis also reported to be effective in aging and chronic diseases, atopic dermatitis, dementia, and cerebrovascular diseases (Lee & Youn, 2002), Therefore, this study tried to confirm if active oxygen in the blood decreased with time after drinking hydrogen water. Materials and Methods 1. Experimental materials ‘The hydrogen water used in the experiment was Few Rich Hydrogen Water", (Hayannamoo Beverage Co., Ltd., Korea), and the properties of this hydrogen water were pH 7.5, dissolved hydrogen amount of 1.5 ppm, ORP - 650 mY. For the control group, purified water using a normal carbon fiter was used for the experiment 2. Selection of clinical trial participants 20 adults over the age of 30 who met the selection criteria ‘were selected, and the study participants were explained in advance. Participants were selected as adults over 30 years of age without risky diseases, and they voluntarily ‘decided to participate in this clinical trial and signed a consent form. Patients who are using special therapeutic agents that affect the immune system, patients with diseases that can affect this study, and other participants ‘who were determined to be not suitable to conduct the study in addition to the above criteria were excluded from this study. 3. Experiment method This experiment was started only for those who consented among the selected clinial trial participants, and before the start ofthe experiment, the clinical study was explained and the preparation meeting forthe clinical trial was held, and the training was conducted, and the experiment schediule were explained to the clinical suibjects and the experimenter in parallel. carried out Prior tothe experiment, the elinial trial participants were Instructed to fast and refrain from excessive exercise or smoking for 4 hours before the experiment. The clinical rial participants were divided into 10 control groups (PW, purified water) and 10 people in the experimental group (HW, hydrogen water), and the experiment was conducted with n=20 per group through a crossover experiment. Both groups were asked to drink at a standard of 12mL/kg, and SOx of blood was collected from the fingertips before drinking and 1 and $ minutes after drinking, and the blood samples were reacted with FORT test kit (Callegari S.p.A., Italy) with a medical photometer (FORM OX, Free Oxygen Radicals Monitor, Callegari S.p.A., Italy) to measure the amount of active ‘oxygen. The analysis principle is that alkoxyl radicals and peroxyl radicals generated by oxidation-reduction of peroxide and hydrogen peroxide by Fe ions combine with CrNHs o form a coloring agent. Tis is a method to confirm using a colorimetric method for measuring the concentration of active oxygen active oxygen according to the degree of color development, and the unit is expressed as FORT unit (FU). Results and Discussion ‘The content of active oxygen in blood measured before drinking, | minute and 5 minutes after drinking to investigate the change of active oxygen according to drinking of hydrogen water is as follows (Figure 1). Before drinking After | minute Aer minates ad Active Oxygen (FU) Figure 1. Changes in active oxygen in the blood after drinking purified water and hydrogen water. PW; integer, HW; number of hydrogens, *p<0.05, **p<0.01 In the case of purified water (PW), there was no statistical difference in the change in active oxygen before drinking at 275.380,8 and 5 minutes after drinking at 275.0478.8 but the active oxygen level of hydrogen water (HW) decreased from 272.768.0 before drinking to 257 after | minute of drinking, and tolpa0.@s-70:0)antef S minutes of drinking, which was sipisficall i Based on these results, the amount 8 converted 10 0 and the amount of active oxygen decreased over time was quantified. As a result, in the case of purified water, there was almost no difference between before drinking and drinking at 1 minute, and it decreased by 0.3422.3 after S minutes of drinking, but there was no statistical significance. In the group drinking hydrogen water, it decreased by 15.7425. after | minute (p<0.01) and decreased by 11.829.3 after $ minutes (p<0.05), so itwas confirmed that hydrogen water had the effect of reducing active oxygen compared to purified water (Figure 2) 10. 2 T Elo i I |) Cp ae ainns z BB| stein i | ao] Bae : = m0. ow va Figure 2. Comparison ofthe amount of ative oxygen eduction inthe ‘blond afer drinking prfed water and hydrogen water. PW; integer, HW; number of hydrogens, *p<0.05, **p<0.01 e 8 I minute Figure 3. Individual changes in active oxygen after 1 minute and 5 minutes of drinking hydrogen water, In addition, in the hydrogen water drinking group, the change in active oxygen at 1 minute and 5 minutes after drinking was checked individually, and the result showed that out ofa total of 20 people, 15 subjects (75.0%) after 1 minute of drinking and 16 subjects (80%) after 5 ‘minutes of drinking showed the level of active oxygen either decreased or maintained (Fig. 3) In this experiment, clinical participants were asked to drink hydrogen water to test the reduction of active oxygen in the blood, and it was confirmed that drinking hydrogen water effectively removes active oxygen from the blood in the human body. To remove active oxygen, various methods are used, such as exercise, meditation, oF intake of antioxidants (Kim etal, 2001; Lee et al., 2003; Hu etal, 2004). Among them, hydrogen has the strongest antioxidant function and can safely and effectively reduce active oxygen in the human body (Southom & Powis, 1988). If people continue to drink hydrogen water, whose antioxidant effect has been confirmed, it is expected that it will be of great help in inhibiting aging and preventing and managing chronic diseases such as cancer and dementia and geriatric diseases. In Korea, which will enter a super-aged society, various studies are being, conducted for people to live a long and healthy life, Among them, hydrogen is expected to play an important role. In Japan, which has already entered a super-aged society, many studies have been done on hydrogen, and it is being used in hospitals as well as the general public to treat patients, So fat, it is used as an auxiliary means rather than a direct effect on the improvement of diseases. ‘The significance of this experiment is that the hydrogen antioxidant research, which had been conducted mainly ‘on animals, was extended to humans and the same antioxidant effect was verified, However, the small ‘number of participants remains a future task. In the future, more research is needed to expand clinical rials, and clarify the relationship with various diseases. Conelusion Recently, the use of hydrogen a an auxiliary means for health promotion and disease treatment is increasing, This is because hydrogen has been reported to be e preventing and treating chronid diseasey-i aging by removing active oxyde. THEtER was intended to confirm throu: active oxygen decreased in the blood over time after drinking hydrogen water. Experimental subjects were selected from adults over 30 years of age without risk diseases who signed the consent form. The experiment ‘was divided into 10 people in the purified water group (PW) and 10 people in the hydrogen water group (HW), and it was set as n=20 per group through a crossover experiment. The participants were asked to drink the experimental water at 12 mL/kg of body weight, and then blood was collected at | and $ minutes to measure the amount of change in active oxygen in the blood, According to the result of the experiment, the level of active oxygen in the hydrogen water drinking group was ‘measured to be 272.7268.0 before drinking hydrogen, ‘water. It was reduced to 257.0:75.0 1 minute after drinking and 260,9270.0 5 minutes after drinking, in the statistical program, significant results were obtained; p<0.01 after 1 minute of drinking and p<0.05 after 5 ‘minutes of drinking. From the result of the experiment, it ‘was confirmed that among 20 people in the hydrogen water drinking group, 15 people (75.0%) after | minute of drinking and 16 people (80%) after 5 minutes of drinking showed a decreased or the same level of active oxygen in the changes in individuals, Therefore, if hydrogen water, whose antioxidant effect has been confirmed, is consumed continuously fora long term, it is expected that it will be of great help in preventing and managing. chronic and geriatric diseases. References Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BN, Saul RA, McCord IM, Harman D: Oxygen radicals and human disease. Ann Intern Med 107 : 526-545, 1987. Freeman BA, Crapo JD: Biology of disease: Free radical and tissue injury. Lav Invest 47 : 412-426, 1982. Harman D: A theory based on free radical chemistry. J Gerontol 11(3) : 298-300, 1956. Hu F, Lu R, Huang B, Ming L: Free radical scavenging activity of extracts prepared from fresh leaves of selected chinese medical plants. Fitoterapia 75: 14-23, 2004. Kim SM, Cho YS, Sung SK: The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sei Technol 33 : 626-632, 2001 Lee IH, Youn JI: Pain relief and satisfaction by hydrotherapy among urban elderly. J Korean Soc Phys Ther 14(3) :209-216, 2002. Lee MY, Kim YK, Ryoo KK, Lee YB, Park ES Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein. Appl Biochem Biotechnol 135(2) : 133-144, 2006. Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH: Screening, of medicinal plant extracts for antioxidant activity, Life Sei 73 : 167-179, 2003. Slater TF: Free radical mechanisms in tissue injury. Biochem J 222(1) : 1-15, 1984, Southorn PA, Powis G: Free radicals in medicine. Il. Involvement in human disease, Mayo Clin Proc 63, 390-408, 1988 Provider] Oonoload by IP 2236222257 at Monday, May 9, 2022 957 PM SHEA VoL. No.2 Korean Joural of Waters 52, 5-28 2016) Par $ So] Selell vlAke Bs} Bet ALS, WEI, ol stat laioem apelaulet saslgnend The Antioxidative Effect of Hydrogen Water Drinking in Blood Eun Sook Jeong, Dong Heui Kim, Kyu Jee Lee” Deparment of Envicrmenal Medical Biology, Yoni Univesiy Worju Colge of Meikine ‘Won, Ganewon 26126, Republic of Koea (Receive September 9, 2016; Revised October 18, 2016: Accept Otber 28. 2016) ‘To confi the antioidaive effect of hyckogen water drinking, the puticipants were divided into tio groups, purified water (PW) drinking group (1-20) and hydrogen war (HWW) drinking group (=20) because of cross tes, in each 10 ina group, Paste water (pH 69, +4320) was produ erly by pipsic lering usin eon ‘carbon ier, sx hydrogen vater (Few Hydrogen Rich Wate™. Hayananoo Beverage Co. Ltd i= wed in this experiment (pH 7, -650mV, hydrogen Spr). Filly ul of blood was collected bel vwer, at | and 2 minutes aler drinking 12mL/kg. ROS in blood vas examined using FORT tet Kit. ROS was showed lover tendeney in HW group than PW group after drnking. and especially lowest tendency in a minute (VOI) and $ mites (20.05), But, thew is no the antioxidative et in purified water. Consequential, drinking hydrogen water is usefl to remove five radials, ROS. “tein icy words: Anixidaton, Drinking, Hydogen water, ROS, Ae aa Oe hel 28, Aslas, ass) Bel § 9] 43) Gaol TAS Ge Yo] Ashe aL ol AE BE Fao] Noni Saal Abs Ve Hol, Sesh} SalBH, alol ot Sal aeloz & 2 Aealne eel Qe Bee] Yasin oot o] — elalst gle Law et a, 206). 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