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BEBAI(LB) Teh!Document No.: MAMB-BMAH-WCLC-RFDH-VPWH
Food and Food Additives Product Item Manufacturing Report
Name Daath
Wook Lee Sepiember 15,1968
Reported by [address Phone
GSijsdone 21-1, Nobyconsongwon,Susonggu, Daegu [Mabie TST
Name Somaya) Business eivalon umber
Voce Coston Co, Li 20210509033
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53, Os Burin-mycon. Viyeons-n, Gyeongsengnam do
Typeated ‘ed beverage — | rodct em manutnciring ep namber | POPTO6OOOTT
Product name few hydrogen mira water
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In accordance with Article 37 Paragraph 5 of the Food Sanitation Act and Article 45 Paragraph I of the
Enforcement Regulations of the same Act, we report the matters pertinent to the manufacture of the food (food
additives).
May 18, 2021
Reported by Woo Jik, Lee
To: Uiryeong County Govemor, Gyeongsangnam-do
Product item report number: 202106090331
Environment and
‘Sanitation
Processed by Depaaiment, | Processed by Seung Yong, Lec | Processed date | December 08,2021
Economy and
Culture BureauDocument No: MAMB-BMAH-WCLC-RFDH-VPWH
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2 | Hydrogen 0.00012%Document No.: MAMB-BMAH-WCLC-RFDH-VPWH
Purpose and how to use
‘Purpose: for consumption, to drink
How to use: drink as itis
How to store and package material
‘Avoid direct sunlight and store in a cool place
Polyethylene (interior)
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SHINHAN LAW & NOTARY OFFICE
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#403-1, 19, Jong-ro, Jongno-gu,
Seoul, Korea
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MEGALB) TOp/at18 Eaforcement Rules ofthe Groundwater Act [Annex: Form No. 12]
Phone (053-622-6198)
No.20222
Certificate of Completion of Construction
‘Name (Casiom BCo,tid) | Date of bin 171511-0014408
{Company name) (Resident registration cubs)
Repoted by
‘Address (location of the main office, in case of a company)
Custom B Co, Ltd, $3, Oso-gil, Burim-myeon, Uiryeong-gun, Gycongsangnam-do
Location
80-2, Dacgok-ri, Burim-myeon, Uiryeong-gun, Gycongsan
Coordinates
Development and use
. longitude
se)
128"21" 46.74
35" 28" 51.25”
Purpose (specific use) | Drinkable/not drinkable
‘Water for industrial use | Drinkable
Excavation depth 123m
[Excavation diameter 200mm
Installed facilities
Planned water intake
150m*/day
Power unit: 75 HP
Inner diameter of discharge pipe 50mm
Pumping facilities
Installed depth: 90m
Pumping capacity 356 m*iday
Other details
I certify that the above facility has been certified for the completion of construction in accordance with Article 14,
Paragraph 3 of the Enforcement Decree of the Act, such as Article 9 of the Underground Water Act.
Jan 12, 2022
Uiryeong County Governor
Official Seal Affixed Hereonmudee NeRaieR mize we)
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May 24, 2022
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personally
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This is hereby attested on this
24th day of May 2022 at this office.
SHINHAN LAW & NOTARY OFFICE
Seoul Central
District Prosecutor's Office
#403-1, 19, Jong-ro, Jongno-gu,
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<3 1S.
Signature of the Notary Public
KIM MOON SOO
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Korea, to act as Notary Public Since
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2lOmam X 297mm
BESAC#) T0alnhCertificate of Authentication
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‘Vaid ei eubmited to fooign mission nthe Republic of Korea,
1a :Repubct Koa
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NOTARIAL CERTIFICATE
SHINHAN LAW & NOTARY OFFICE
2022 - 8825
#403-1, 19, Jong-ro , Jongno-gu,
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210mm X 27mm
BEG ACE) T0e/ni1 Enforcement Rules ofthe Groundwater Act [Annex: Form No, 12],
No.2
Certificate of Completion of Construction
| Name (Custom BCo,L48) | Date ofbinh 171511-0014408
(Company nam) (Resident egsration number)
Reported by
Address (location of the main office, in case of a company)
Custom B Co,Ltd, $3, 0s0-gl, Burim-myeon, Uiryeong-gun, Gyeongsangnam-do
Phone (053-622-6198)
Location
79-5, Daegok-ti, Burim-myeon, Uiryeong-gun, Gyeongsangnam-do
Development and use jcominnss
longitude, latitude) Purpose (specific use) | Drinkable/not drinkable
128" 21°47.32" ‘Water for industrial use | Drinkable
35" 28" 50.84"
[Excavation depth 122m —_| Excavation diameter 250mm
Installed facilities
Planned water intake 4om’/day
| Power unit: 7.5 HP Inner diameter of discharge pipe 50mm
Pumping facilities
Installed depth: 90m Pumping capacity 356 m*/day
Other details
I certify that the above facility has been certified for the completion of construction in accordance with Article 14,
Paragraph 3 of the Enforcement Decree of the Act, such as Article 9 of the Underground Water Act.
Jan 12, 2022
Uiryeong County Governor
/S/Official Seal Affixed Hereonmasse wepulen m2eMel
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20224 058 249 May 24, 2022
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5 2022 Al 825% Registered No. 2022-8825
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BVSdq wat 2 appeared before me, confirmed th:
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arith. original and subscribed hii
202214 058 249 This is hereby attested on this
o] ARR AAA] 9] See}, 24th day of May 2022 at this office.
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Seopa MARRAS Seoul Central
District Prosecutor's Office
ANSSY) SET FHI, 403-1E #403-1, 19, Jong-ro, Jongno~gu,
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Les tt S.
Beyqusy gk = Signature of the Notary Public
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PAPAL U7hAS 71325E41] 718°] This office has been authorized by the
2005'4 019 039 WEE AY2=E¥=e] — Minister of Justice, the Republic of
SEU USS VY AS U7 WI. Korea, to act as Notary Public Since
3, Jan. 2005 Under Law No.325.
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MEBANE) 70e/tKorean Journal of Waters 5(2), 25-28 (2016)
The Antioxidative Effect of Hydrogen Water Drinking in Blood
Eun Sook Jeong, Dong Heui Kim, Kyu Jae Lee
Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine,
Wonju, Gangwon 26426, Republic of Korea
(Received September 9, 2016; Revised October 18, 2016; Accepted October 28, 2016)
To confirm the antioxidative effect of hydrogen water drinking, the participants were divided into two groups,
purified water (PW) drinking group (n=20) and hydrogen water (HW) drinking group (n=20) because of cross
test in each 10 in a group. Purified water (pH 6.9, +432mV) was produced only by physical filtering using,
‘commercial carbon filter, and hydrogen water (ew Hydrogen Rich Water™, Hayannamoo Beverage Co. Ltd.,
Korea) was used in this experiment (pH 7.5, -650mY, hydrogen .Sppm). Fifty ul. of blood was collected before
drinking water, at | and 2 minutes after drinking I2mL/kg, ROS in blood was examined using FORT test kit.
ROS was showed lower tendency in HW group than PW group after drinking, and especially lowest tendency in
‘a minute (p<0.01) and 5 minutes (p<0.05). But, there is no the antioxidative effect in purifi
Consequentially, drinking hydrogen water is useful to remove active oxygen, ROS.
Key words : Antioxidation, Drinking, Hydrogen water, ROS,
Introduction
In modem society, life expectancy is increasing, and
various diseases are occurring due to complex social
structure and stress, and government-level efforts and
medical support to manage them are also on the rise
However, he treatment of modern medicine to improve
various diseases caused by work stress, modem diet, and
social anxiety is showing limitations. In particular, in
Korea, which is entering an aging society, the number of
elderly patients is rapidly inereasing, and most of the
diseases are chronic diseases, which incur high costs. The
problem of medical expenses for chronic diseases is
emerging as a big problem for families and the country.
Ithas been found that active oxygen is the main cause of
chronic diseases. Active oxygen is an oxygen compound
that is generated not only in the metabolic process of
body cells of animals and plants, but also in large
amounts by external environmental pollution such as
ozone, nitrie oxide, ultraviolet rays, and smoking, These
are known to cause aging, arteriosclerosis, dementia,
cancer, ete. (Lee et al., 2006). In general, in the normal
human respiration process, about 95% of oxygen is used
to make energy, and 4-5% is used to produce active
oxygen. The generated active oxygen play an important
role in the cell signaling system or generation, act as a
cell messenger, and regulate oxidation/reduction reactions
(Harman, 1956). They promote the activation of enzymes,
drug detoxification and glycogen synthesis. If there is an
appropriate amount of these, it protects the body from
bacteria and foreign substances. On the other hand, when
they occur excessively, they attack normal cells
indiscriminately, causing chronic diseases, and becoming
the main cause of various diseases such as cancer,
diabetes, dementia, and aging (Freeman & Crapo, 1982;
Cross et al, 1987). More than 90% of modem disease
causes are active oxygens, and they have been found to be
the cause of aging (Slater, 1984). Hydrogen is being used
as a powerful antioxidant that removes active oxygens.
Hydrogen is reported to be effective in both drinking and
inhalation on the human body, and itis also reported to be
effective in aging and chronic diseases, atopic dermatitis,
dementia, and cerebrovascular diseases (Lee & Youn,
2002),
Therefore, this study tried to confirm if active oxygen in
the blood decreased with time after drinking hydrogen
water.
Materials and Methods
1. Experimental materials
‘The hydrogen water used in the experiment was Few Rich
Hydrogen Water", (Hayannamoo Beverage Co., Ltd.,
Korea), and the properties of this hydrogen water were
pH 7.5, dissolved hydrogen amount of 1.5 ppm, ORP -
650 mY. For the control group, purified water using a
normal carbon fiter was used for the experiment2. Selection of clinical trial participants
20 adults over the age of 30 who met the selection criteria
‘were selected, and the study participants were explained
in advance. Participants were selected as adults over 30
years of age without risky diseases, and they voluntarily
‘decided to participate in this clinical trial and signed a
consent form. Patients who are using special therapeutic
agents that affect the immune system, patients with
diseases that can affect this study, and other participants
‘who were determined to be not suitable to conduct the
study in addition to the above criteria were excluded from
this study.
3. Experiment method
This experiment was started only for those who consented
among the selected clinial trial participants, and before
the start ofthe experiment, the clinical study was
explained and the preparation meeting forthe clinical trial
was held, and the training was conducted, and the
experiment schediule were explained to the clinical
suibjects and the experimenter in parallel. carried out
Prior tothe experiment, the elinial trial participants were
Instructed to fast and refrain from excessive exercise or
smoking for 4 hours before the experiment.
The clinical rial participants were divided into 10 control
groups (PW, purified water) and 10 people in the
experimental group (HW, hydrogen water), and the
experiment was conducted with n=20 per group through a
crossover experiment. Both groups were asked to drink at
a standard of 12mL/kg, and SOx of blood was collected
from the fingertips before drinking and 1 and $ minutes
after drinking, and the blood samples were reacted with
FORT test kit (Callegari S.p.A., Italy) with a medical
photometer (FORM OX, Free Oxygen Radicals Monitor,
Callegari S.p.A., Italy) to measure the amount of active
‘oxygen. The analysis principle is that alkoxyl radicals and
peroxyl radicals generated by oxidation-reduction of
peroxide and hydrogen peroxide by Fe ions combine with
CrNHs o form a coloring agent. Tis is a method to
confirm using a colorimetric method for measuring the
concentration of active oxygen active oxygen according
to the degree of color development, and the unit is
expressed as FORT unit (FU).
Results and Discussion
‘The content of active oxygen in blood measured before
drinking, | minute and 5 minutes after drinking to
investigate the change of active oxygen according to
drinking of hydrogen water is as follows (Figure 1).
Before drinking
After | minute
Aer minates
ad
Active Oxygen (FU)
Figure 1. Changes in active oxygen in the blood after
drinking purified water and hydrogen water.
PW; integer, HW; number of hydrogens, *p<0.05,
**p<0.01
In the case of purified water (PW), there was no statistical
difference in the change in active oxygen before drinking
at 275.380,8 and 5 minutes after drinking at 275.0478.8
but the active oxygen level of hydrogen water (HW)
decreased from 272.768.0 before drinking to 257
after | minute of drinking, and tolpa0.@s-70:0)antef S
minutes of drinking, which was sipisficall i
Based on these results, the amount 8
converted 10 0 and the amount of active oxygen decreased
over time was quantified. As a result, in the case of
purified water, there was almost no difference between
before drinking and drinking at 1 minute, and it decreased
by 0.3422.3 after S minutes of drinking, but there was no
statistical significance. In the group drinking hydrogen
water, it decreased by 15.7425. after | minute (p<0.01)
and decreased by 11.829.3 after $ minutes (p<0.05), so
itwas confirmed that hydrogen water had the effect of
reducing active oxygen compared to purified water
(Figure 2)
10.
2 T
Elo
i I
|) Cp ae ainns
z BB| stein
i | ao] Bae :
= m0.
ow va
Figure 2. Comparison ofthe amount of ative oxygen eduction inthe
‘blond afer drinking prfed water and hydrogen water.
PW; integer, HW; number of hydrogens, *p<0.05,
**p<0.01e
8
I minute
Figure 3. Individual changes in active oxygen after 1 minute and 5 minutes of drinking hydrogen water,
In addition, in the hydrogen water drinking group, the
change in active oxygen at 1 minute and 5 minutes after
drinking was checked individually, and the result showed
that out ofa total of 20 people, 15 subjects (75.0%) after
1 minute of drinking and 16 subjects (80%) after 5
‘minutes of drinking showed the level of active oxygen
either decreased or maintained (Fig. 3)
In this experiment, clinical participants were asked to
drink hydrogen water to test the reduction of active
oxygen in the blood, and it was confirmed that drinking
hydrogen water effectively removes active oxygen from
the blood in the human body. To remove active oxygen,
various methods are used, such as exercise, meditation, oF
intake of antioxidants (Kim etal, 2001; Lee et al., 2003;
Hu etal, 2004). Among them, hydrogen has the strongest
antioxidant function and can safely and effectively reduce
active oxygen in the human body (Southom & Powis,
1988). If people continue to drink hydrogen water, whose
antioxidant effect has been confirmed, it is expected that
it will be of great help in inhibiting aging and preventing
and managing chronic diseases such as cancer and
dementia and geriatric diseases. In Korea, which will
enter a super-aged society, various studies are being,
conducted for people to live a long and healthy life,
Among them, hydrogen is expected to play an important
role. In Japan, which has already entered a super-aged
society, many studies have been done on hydrogen, and it
is being used in hospitals as well as the general public to
treat patients, So fat, it is used as an auxiliary means
rather than a direct effect on the improvement of diseases.
‘The significance of this experiment is that the hydrogen
antioxidant research, which had been conducted mainly
‘on animals, was extended to humans and the same
antioxidant effect was verified, However, the small
‘number of participants remains a future task. In the
future, more research is needed to expand clinical rials,
and clarify the relationship with various diseases.
Conelusion
Recently, the use of hydrogen a an auxiliary means for
health promotion and disease treatment is increasing, This
is because hydrogen has been reported to be e
preventing and treating chronid diseasey-i
aging by removing active oxyde. THEtER
was intended to confirm throu:
active oxygen decreased in the blood over time after
drinking hydrogen water. Experimental subjects were
selected from adults over 30 years of age without risk
diseases who signed the consent form. The experiment
‘was divided into 10 people in the purified water group
(PW) and 10 people in the hydrogen water group (HW),
and it was set as n=20 per group through a crossover
experiment. The participants were asked to drink the
experimental water at 12 mL/kg of body weight, and then
blood was collected at | and $ minutes to measure the
amount of change in active oxygen in the blood,
According to the result of the experiment, the level of
active oxygen in the hydrogen water drinking group was
‘measured to be 272.7268.0 before drinking hydrogen,
‘water. It was reduced to 257.0:75.0 1 minute after
drinking and 260,9270.0 5 minutes after drinking, in the
statistical program, significant results were obtained;
p<0.01 after 1 minute of drinking and p<0.05 after 5
‘minutes of drinking. From the result of the experiment, it
‘was confirmed that among 20 people in the hydrogen
water drinking group, 15 people (75.0%) after | minute of
drinking and 16 people (80%) after 5 minutes of drinking
showed a decreased or the same level of active oxygen in
the changes in individuals, Therefore, if hydrogen water,
whose antioxidant effect has been confirmed, is
consumed continuously fora long term, it is expected that
it will be of great help in preventing and managing.
chronic and geriatric diseases.
References
Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BN,Saul RA, McCord IM, Harman D: Oxygen radicals and
human disease. Ann Intern Med 107 : 526-545, 1987.
Freeman BA, Crapo JD: Biology of disease: Free radical
and tissue injury. Lav Invest 47 : 412-426, 1982.
Harman D: A theory based on free radical chemistry. J
Gerontol 11(3) : 298-300, 1956.
Hu F, Lu R, Huang B, Ming L: Free radical scavenging
activity of extracts prepared from fresh leaves of
selected chinese medical plants. Fitoterapia 75: 14-23,
2004.
Kim SM, Cho YS, Sung SK: The antioxidant ability and
nitrite scavenging ability of plant extracts. Korean J
Food Sei Technol 33 : 626-632, 2001
Lee IH, Youn JI: Pain relief and satisfaction by
hydrotherapy among urban elderly. J Korean Soc Phys
Ther 14(3) :209-216, 2002.
Lee MY, Kim YK, Ryoo KK, Lee YB, Park ES
Electrolyzed-reduced water protects against oxidative
damage to DNA, RNA, and protein. Appl Biochem
Biotechnol 135(2) : 133-144, 2006.
Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH: Screening,
of medicinal plant extracts for antioxidant activity, Life
Sei
73 : 167-179, 2003.
Slater TF: Free radical mechanisms in tissue injury.
Biochem J 222(1) : 1-15, 1984,
Southorn PA, Powis G: Free radicals in medicine. Il.
Involvement in human disease, Mayo Clin Proc 63,
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Korean Joural of Waters 52, 5-28 2016)
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The Antioxidative Effect of Hydrogen Water Drinking in Blood
Eun Sook Jeong, Dong Heui Kim, Kyu Jee Lee”
Deparment of Envicrmenal Medical Biology, Yoni Univesiy Worju Colge of Meikine
‘Won, Ganewon 26126, Republic of Koea
(Receive September 9, 2016; Revised October 18, 2016: Accept Otber 28. 2016)
‘To confi the antioidaive effect of hyckogen water drinking, the puticipants were divided into tio groups,
purified water (PW) drinking group (1-20) and hydrogen war (HWW) drinking group (=20) because of cross tes,
in each 10 ina group, Paste water (pH 69, +4320) was produ erly by pipsic lering usin eon
‘carbon ier, sx hydrogen vater (Few Hydrogen Rich Wate™. Hayananoo Beverage Co. Ltd i=
wed in this experiment (pH 7, -650mV, hydrogen Spr). Filly ul of blood was collected bel
vwer, at | and 2 minutes aler drinking 12mL/kg. ROS in blood vas examined using FORT tet Kit. ROS was
showed lover tendeney in HW group than PW group after drnking. and especially lowest tendency in a minute
(VOI) and $ mites (20.05), But, thew is no the antioxidative et in purified water. Consequential,
drinking hydrogen water is usefl to remove five radials, ROS.
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icy words: Anixidaton, Drinking, Hydogen water, ROS,
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