Laboratory Exercise # 4 (Qualitative Tests for Lipid)

Degree Program: BSN Year level/section: 1- J

Group members:
Christine Marie Calanza
Hannah Gabrielle Albarina
Alessandra Louisse Atabay
Narena Bonite
Yari Yzza Belle Calawod
Jhon Edward Badilles
Jenny Rose Carbonilla
Harylle Alyssa Amor
Kianna Akita
Threshyl Diana Jazzmine A. Carpio

Purpose of the laboratory exercise (based on the content of the video):

● The purpose of the laboratory exercise is to allow students to observe, and witness the
reactions that will take place on each test. In this way, they will truly learn how
substances react with addition of different chemicals. Learning will be more accurately
acquired once it will be performed by the students hence performing the laboratory
exercise.

Briefly describe the procedure used in the activity. (Brief in this context means short but
it comprehendly covers the needed information):
● Solubility in Water
Take a tiny amount of the desi ghee, veggie ghee, and refined oil samples accordingly,
test tubes A, B, and C.
1) Fill test tubes A, B, and C with distilled water in tiny amounts.
2) Thoroughly shake each test tube.
3) Keep track of observations.
● Solubility with alcohol
1) Place a tiny amount of the vegetable ghee, desi ghee, and refined oil samples
accordingly, test tubes A, B, and C.
2) Drop a few drops of ethyl alcohol into each test tube using a dropper.
3) Thoroughly shake each test tube.
4) Prior to heating the test tubes, note observations.
5) Heat the water in a beaker using the Bunsen burner and triangular tripod support.
6) Put the test tubes in a beaker of boiling water.
7) After the test tubes have been heated, note observations.
● Solubility with Chloroform
1) Place a tiny amount of the vegetable ghee, desi ghee, and refined oil samples in
 accordingly, test tubes A, B, and C.
2) Drop little volumes of chloroform into test tubes A, B, and C using a dropper.
3) Thoroughly shake each test tube.
4) Keep track of observations.
● Translucent Spot Test
1) Scoop up a little amount of the desi ghee sample with a spatula.
2) Move it to a piece of filter paper.
3) Use a different filter to press the substance.
4) Conduct the experiment once more using refined oil and vegetable ghee. Place the
Tilter papers in front of a lit candle and move them into position.
6) Document findings
● Acrolein Test
1) Place a tiny amount of the vegetable ghee, desi ghee, and refined oil samples in
accordingly, test tubes A, B, and C.
2) In test tubes A, B, and C, add small amounts of crystals of potassium bisulfate.
3). Heat each test tube using the tongs over the Bunsen burner.
4) Keep track of observations.
● Baudouin Test
1) Place a tiny amount of the melted samples of desi ghee and vanaspati ghee in A and
B, two test tubes, respectively.
2) Drop little volumes of strong hydrochloric acid into the test with dropper B and A
tubes.
3) Utilizing a different dropper, apply 2% furfural solution in alcohol in tiny doses to the
containers A and B.
4) Give the test tubes a thorough shake and set them aside for five to ten minutes.
5) Keep an observation log.
● Cholesterol Test
1) Select the A and B test tubes.
2) Drop little volumes of chloroform into test tubes A and B using a dropper.
3) Fill test tubes A and B with modest volumes of cotton seed oil using a different
dropper test tube B containing linseed oil.
4) Thoroughly shake the test containers.
5) Add a few drops of Huble's reagent to each test tube using a different dropper.
6) Take notes on what you see.

Results of the activity/experiment as described in the video:

● Solubility test

Sample H2O Alcohol Chloroform

Desi ghee The desi ghee is Desi ghee produces Turns out Desi
immiscible in water a lower layer after Ghee is miscible,
after adding a little adding a small after pouring a
amount of distilled amount of alcohol small quantity of
water and and shaking the chloroform to it
thoroughly shaking test tube. and shaking the
the test tube. test tube.

Vegetable Ghee The vegetable ghee Vegetable ghee Turns out

is immiscible in forms a lower layer vegetable ghee is
water after adding a after adding a small miscible, after
tiny amount of amount of alcohol pouring a small
distilled water and and shaking the quantity of
thoroughly shaking test tube. chloroform to it
the test tube. and shaking the
test tube.

The refined oil Refine oil develops Turns out refined

Refine Oil becomes a lower layer after oil is miscible, after
immiscible in water adding a little pouring a small
after adding a tiny amount of alcohol amount of
amount of distilled and shaking the chloroform to it
water and test tube. and shaking the
thoroughly shaking test tube.
the test tube.

● Translucent Spot Test

Samples Observations

Desi ghee Desi ghee and distilled water are

immiscible after being added to the
sample and shaken, which indicates
that they cannot be combined to create
a homogeneous mixture.

Vegetable ghee Vegetable ghee and distilled water are

immiscible after being added to a
sample and shaken, which indicates
that they cannot be combined to form a
homogenous product.

Refined oil Refined oil and distilled water are

immiscible after being added to a
sample and shaken, which indicates
that they cannot be combined to create
a homogeneous product.
● Acrolein Test

Samples Observations

Desi ghee A pungent irritating odor of acrolein.

Vegetable ghee A pungent irritating odor of acrolein.

Refined oil A pungent irritating odor of acrolein.

● Baudouin Test

Samples Observations

melted desi ghee After adding a modest amount of

concentrated bydrochloric acid sinks,
giving the impression that the color is
gradient. And following the release of a
furfural solution, it also
remains below and sinks.

melted vanaspati ghee After pouring a small quantity of

concentrated hydrochloric acid it sinks
down and creates bubbles. And after
dropping a furfural solution it also sinks
down then after mixing them it
eventually turned into rose-red color.

● Cholesterol test

Samples Observations

Salkawski test When the sulfuric acid is added to the

cholesterol solution and shaken, the
color of the liquid starts to change
from light to dark red.

Liebermann-Burchard test In a centrifuge tube, 5 mL of

Liebermann-Burchard reagent was
prepared. The reagent was made of
acetic acid, acetic anhydride, and
sulfuric acid. The Liebermann
Burchard reagent was added slowly to
egg extract and started to react
violently, and after a few seconds, the
 green color started appearing in the
reaction. The same procedure was
applied to the rest of the extracts,
which were walnut and blood, and all
of them turned green, which also
happened to the egg extract.

Reaction to the video (You may state here whether you like or dislike the video based on
the manner how it was presented. Provide reasons for your views or opinion) (1
paragraph will do for this part):

The video that we saw is very clear and we can understand the lesson thoroughly though some
of the experiments that were shown were quite difficult to understand but since she explained it
very well the topics seem to be easy to understand. The video is interesting and it answers
some of our questions that we have kept in our minds.

The person who performed the experiments did a great job for she follows the rules when doing
an experiment. Her explanation is very clear and easy to understand. We also like the way she
performed the experiments very carefully.
Overall, the video is really good and knowledgeable enough for us.

Important learnings acquired from the video (1 paragraph will do for this part):

The important learnings we acquired from this video are the different reactions of substances
depending on the chemical and its amount that was dropped to each tube. We learned that we
should always be cautious as to what substance we will mix and immediately observe the
reaction after to record accurate data. Measurement and quantity of the substance must be
carefully observed to avoid different outcomes of the experiment. Safe handling of chemicals
and proper usage of lab gowns will be observed to prevent injuries and keep everyone safe.