CHAPTER 3

Structure and organizaton of

animal cell and tssue culture
Kinetics of
cell growth
Advantages of Animal cell culture
Here are a few of the benefits of using animal cell culture:
1.Cell culture is superior to similar methods of biotechnology because it allows
for the alteration of various physiological and physiobiological conditions such
as pH, temperature and Osmotic pressure.
2.Animal cell culture is a great way to study issues that study cell metabolism
and helps to understand the cell biochemistry.
3.It also permits observation of the effects of different chemicals like drugs and
proteins on various cell types.
4.The results of animal cell culture are consistent regardless of the type of cell
that is utilized.
5.This technique can also facilitate the identification of various cells on the
basis in the appearance of marker, such as molecules, or through the
karyotyping.
6.The usage of animal cells for testing and other purposes is a barrier to the
animal experiments.
7.Animal cell cultures can be utilized to produce massive amounts of
antibodies and proteins that otherwise would require an investment of a
substantial amount.
Disadvantages of Animal cell culture
Although the culture of animal cells is used as an advanced technology but there
are some drawbacks that are associated with this method.
•It is a highly specialized method that requires experienced personnel as well as
the right conditions for aseptic treatment. It is a costly procedure because it
requires expensive equipment.
•Subcultures of the cell line could result in different properties when different
from that of the initial strain.
•The process produces a tiny amount of recombinant proteins which increases the
cost of the procedure.
•Mycoplasma-related contamination and viral infections are common and are hard
to identify and manage.
•The cells produced by this technique lead to instability due to the occurrence of
aneuploidy chromosomal constitution.