Department of Genetic Engineering and Biotechnology, CU Curriculum at a Glance for the Degree of Bachelor of Science (Honours) in Genetic Engineering and Biotechnology [This curriculum is applicable to the students admitted under thd four year B. Sc. (Honours) program in 2021-2022 and 2022-2023. ll. Overview of the Department of Genetic Engineering and Biotechnology {The Department of Genetic Engineering and Biotechnology (GEB as started its journey as an essential area of knowledge i understanding molecular and genetic basis of life as multidisciplinary subject. GEB was regarded as a research oriente subject to find out the genetic constituents and its relate echanism of both plants and animals including humans. Moreover, genetic engineering proposes solutions for qualitativ d quantitative improvements of life. Gradually it turns into a Tpplied scierice and it proved its application in agriculture and food, health, disease diagnosis, bioremediation and biotech, industries, and in biopharmaceuticals. However, with the advent of genetic engineering in the last thirty years, the Genetic Enginee! have been able not only to understand the life process but t manipulate it according to specific needs. In fact, the emplcation, jof Genetic Engineering and Biotechnology made a visible impact lin the advancement of health, agriculture, livestock production. food security, forensic science and biopharmaceuticals sectors of Bangladesh which ultimate plays a vital role to contribute the economic development of the country. Within a short span of time. any public Universities including some private Universities havd understood the immense power and importance of Genetig Engineering and Biotechnology and its growing demand both at home and abroad. Therefore, it is importantly necessary to produce more graduate in this subject to pave the demand; hence established this department at many Universities including public Universities. B. Sc. (Hons.) curriculum for the session 2021-2022 and 2022-2023 3iment of Genetic Engineering and Biotechnology, CU Depart As the importance of GEB to the national development was becoming clear to the academics and Government and there Was growing demand of expert graduates in this area, the University authority with the directive of the Government decided to establish the department in CU on 30 October, 2004 and started its activity to produce graduates to meet the local and international demand, U has been offering 4 year B.Sc, Since its inception, the GEB-C (Honours), | year MS., 2 years M.Phil, and 3 years Ph.D degree programs from 2004-2005 session. 2. Vision of the Department of Genetic Engineering and Biotechnology To be a department of excellence for academic and research in biosciences that will infuse highest cognitive skills to serve educational and research institute along with humanity to create knowledge-based society. 3. Mission of the Department of Genetic Engineering and Biotechnology The department of GEB generates skilled manpower through cutting edge research, innovation, creativity, academic excellence and interactive teaching-learning environment. 4. Objectives of the Program 1) To provide well-structured and need based curriculum. 2) To make over research facilities of GEB, CU as a centre of excellence in Biotechnology. 3) To facilitate opportunities for extracurricular activities that 4 enhances generic skills of GEB graduates. 5 in peal nia! and technical quality of graduates. wane en social ethical implications of Biotechnology tha' responsibilities of GEB graduates.Department of Genetic Engineering and Biotechnology, CU 5. Intended Learning Outcomes (ILO) of the Program The graduates will compete and obtain job in research institutes, food, pharmaceuticals, agro-based companies, diagnostic labs, Bangladesh Government service through BCS etc. though this subject is yet to be included in the BCS as a separate cadre. Some of the graduates will enter into postgraduate study and also go abroad for higher studies. 5. Course Outline The program of study for B. Sc. (Honours) degree in Genetic Engineering and Biotechnology shall extend over 4 (four) academic years and shall be completed by a student in not more than 6 (six) academic years. The program shall be an integration of major and related courses carrying a total of 4000 marks. There shall be major courses of 2700 marks, related courses of 700 marks, sessional of 400 marks and viva-voce of 200 marks. Major courses shall consist of 27 theory courses of 1950 marks and 12 practical courses of 750 marks. There shall be 8 related theory courses of 500 marks and 4 related practical courses of 200 marks. Courses of 25, 50, 75 and 100 marks shall carry 1, 2, 3 and 4 credits, respectively. 25-35% of the total of theoretical and practical marks shall be allotted for practical in all subject. 20% of the practical marks will be distributed as practical class performance, and other 80% on the basis of annual practical examination [Source: Ordinance 7 (i-v)].T= Thearetical, P*= Practical. Sessional: Sessional shall include slides and specimen collection. class attendance, class test/tutorial examination, field study. excursion, practical note book, reports etc. Special English Course: Apart from the credit courses mentioned above, one English course of 100 marks must be taken in the [st year of the program. Points secured in this course in excess of 12.0 shall be added in calculated CGPA. Medium of Instruction and Examinations: The medium of instruction and examinations will be English.wepurimens wy ueneuc Engineering and Biotechnology, CU 6. Curriculum Sequence of Four Year B. Sc. (Honours) Program Curriculum for First Year B. Sc. (Honours) Course Session: 2021-2022 and 2022-2023 (Year of Examination: 2022 and 2023) Major Theory Courses 2325 Related Theory Courses | : 250 Major Practical Courses |: 125 Related Practical Courses |: 100 Sessional 7100 Viva-voce £50) Title Marks Credit Hours | Major Theory Courses: GEB.101: | Introductory Genetic Engineering and Biotechnology | __75 3 GEB.102; | Cell Biology and Cytology 5 3 GEB.103: | Genetics and Cytogenetics 50 t GEB.104; | Basic Biochemistry 5 3 GEB.105: | Basic Microbiology 30 2 Total : 325 13 Related Theory Courses: GEB.106: | Basic Biology 5 3 GEB.107: | Elementary Mathematics and Calculus 50 2 GEB. 108: | Biophysical Chemistry 50 2 GEB. 109: | Fundamentals of Computer and IT 5 3 Total : 250 10 Major Practical Courses: GEB. 110: | A. Introductory Genetic Engineering and 5 3 Biotechnology (60+15*) B. Cell Biology and Cytology C. Genetics and Cytogenetics GEB.111: | A. Basic Biochemistry 50 2 B. Basie Microbiology (40+10*) Total: 15 3 Related Practical Courses: GEB. 112: | Basic Biology 50 a (40+10*) GEB.113: | A. Biophysical Chemistry 50 2 B. Fundamentals of Computer and IT (40+10*) Total: © 100 4 GEB.114: |] Sessional 100 4 [Class Test 40; Practical Note Book 20; Field Study (Others) 20; Class Attendance 20) _ GEB.115: | Viva-voce 50 2 Grand Total: 950 38 English Course J: Compulsory non-credit 100 4 *Practical Class Performance. 1 credit hour = 15 lectures, 1 lecture = 60 minutes, 1 credit hour = 25 marks B. Sc. (Hons.) curriculum for the session 2021-2022 and 2022-2023“er ung una BIotechnology, CU Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong Curriculum for First Year B. Sc. (Honours) Course Session: 2021-2022 and 2022-2023 (Year of Examination: 2022 and 2023) Major Theory Courses | +325 Related Theory Courses _| : 250 Major Practical Courses_| : 125 Related Practical Courses | : 100 Sessional 7100 Viva-voce 250 Title Marks | Credit Hours Major Theory Courses: GEB.101: [Introductory Genetio Engineering and Biotechnology [75 3 GEB.102: | Cell Biology and Cytology 75 3 Genetics and Cytogenetics 50 2 Basic Biochemistry _ 5 3 GEB.105: | Basic Microbiology 50 2 Total : 325 3 Related Theory Courses: GEB.106:__| Basic Biology 75 3 Elementary Mathematics and Calculus 30 2 Biophysical Chemistry 50 2 Fundamentals of Computer and IT 5 3 Total : 250 10 Major Practical Courses: GEB.110: | A. Introductory Genetic Engineering and 5 3 Biotechnology (60+15%) B. Cell Biology and Cytology C. Genetics and Cytogenetics GEB.111: | A. Basic Biochemistry 30 2 B. Basic Microbiology (40+10*) Total: 125 3 Related Practical Course GEB. 112: | Basic Biology 50 2 (4o+10*) GEB.113: | A. Biophysical Chemistry 50 2 B. Fundamentals of Computer and IT (40+10*) Total: 100 4 GEB.114: | Sessional 100 4 {Class Test 40; Practical Note Book 20; Field Study (Others) 20; Class Attendance 20) GEB. I ‘Viva-voce 50 2 Grand Total 950 38 English Course I: Compulsory non-credit 100 4 “Practical Class Performance. 1 credit hour = 15 lectures, 1 lecture = 60 minutes, | credit hour = 25 marks B. Sc. Hons.) curriculum for the session 2021-2022 and 2022-2023 i= tment of Genetic Engineering and Biotechnology, CU rtm: Depar Course: GEB. 101: Introductory Genetic Marks: Credit Hour: Lecture No, OUTSE: » . Engineering and Biotechnology 5 03 45 oe ide knowledge on the basic concept, tools This course is designed to provi and techniques involved as wel genetic engineering and biotechnology. 1 as the prospects and applications of Course Objectives: : ay : e Cone pause the basics of genetic engineering and biotechnology int wil a id techniques ‘© Acquaint with the relevant molecules, tools ani 1 ; . Understand the application of the knowledge of genetic engineering and biotechnology in the modern life : : Familiarize with the recent advancements and practice of the genetic engineering and biotechnology Intended Learning Outcomes (ILOs): At the end of the course the students will be able to- 1. Describe the fundamentals of genetic engineering and biotechnology. 2. Explain the characteristics and role of the molecules, tools and techniques involved in biotechnology. 3. Analyze the application of biotechnology. 4. Explain the recent practice and prospects of biotechnology. Course Content: 1. Biotechnology: Definition, history and multidisciplinary nature of biotechnology and genetic engineering, present and future prospects of genetic engineering and biotechnology in Bangladesh. 2. Nucleic Acids: Classification and structural components, their occurrence, isolation, purification and molecular weight determination. 3. DNA: Structure of DNA, types, different physico-chemical properties, such as Tm value, Cot value, hybridization kinetics; different conformations of DNA; sequences complexity, tandem sequences, palindromic sequence, cruciform structure, biological properties and functions of DNA. 4. RNA: Structure, types, function and synthesis of RNAs (primary, secondary and tertiary). $i Introduction to Plant and Animal Cell and Tissue Culture Techniques: History, basic techniques, culture media, culture ae, totipotency, embryogenesis and organogenesis, peli micropropagation, protoplasts isolation and fusion; somatic brids, stem cells, hybridoma and animal cell culture. B. Sc. (Hons,) curriculum for the session 2021-2022 and 2022-2023 12Department of Genetic Engineering and Biotechnology, Cl 6. Tools of Genetic Engineering: Restricti i andother enzymes useful in gene cloning, eDNA uses of plemid snd phages as oe ve sear for eukaryotes, gene libraries, use of marker genes, use of Agrobacteriu ic engineering in pla Current Trends in Biotechnolo i for genetic engineering in plants. (a) Scope of biotechnology for research; (b) Agriculture related applications: Plant character amenable to change by biotechnology. seed quality, photosynthesis, herbicides resistance; (c) Medicine related applications: Commercial synthesis of hormones, vaccines etc. gene therapy, and disease diagnosis; (d) Microbial application: Large- scale preparation of organic chemicals, biomining, microorganisms as feed of livestock; (¢) Biotechnology in service of environment related application: pollution control, waste disposal, and biogas; (f) Livestock improvement: Dairy products, meat quality; (g) Monoclonal antibodies and their applications. 7 References 4, Recombinant DNA: A Short Course: Watson, J.D., Tooze, J. and Kurtz, D.T. 1983, Scientific American Books, New York. 2. Principles of Gene Manipulation: Old, R.W. and Primrose, S.B: 3. A Text Book of Biotechnology: Dubey, R.C 4, Biotechnology: John, E. Smith 5, Biotechnology - A New Industrial Revolution: Steven, P, George Braziller Inc. USA. 6. Introduction to Genetic Engineering: Desmond S.T. Nicholl. 7. Biotechnology in Agriculture: Chopra, V.C. Teaching Strategy: Lecture: The major way of instruction is the interactive lecture. Teacher can use different teaching aids including audio-visual systems to make classes understandable and lively. Assessment Strategy: 1. Interim Assessment: Maximum two/three assessments shall be conducted for half or full course respectively, which is known as class test. The date and topic will be scheduled and announced by the respective course teacher(s). ‘ 2. Final Examination: Will be conducted as scheduled by the controller of examination of CU on full course content (total 50/75 marks). Students will have to answer at least five out of eight questions. 3. Examination of Answer Script: Answer scripts will be examined by two examiners following double blind evaluation system an difference of above 15% marks will be third examined. 3 B. Se. Hons.) curriculum for the session 7021-2022 and 2022-2023 1Engineering and Biotechnology, CU nt of Genetic Departm _— Cell Biology and Cytology arks: Credit Hour: Course: GEB. 102: Cell Biology i ma ~ 4§ Rationale: ned to provide fundamental concepts of cell py, This ot seorganization of eukaryotic cell organelles a bic , eancepts of cell division. of Objectives? ; ' sabe wunlize cell biology t© differentiate eukaryotic and prokaryote Seder the ultrastructure and functions of eukaryotic cell ell : nomi knowledge on the structure, classification and ton of al eee eeend nd meiosis cell division # Understand the mitosis a d e Acquaint with biophysical phenomena (e.g. colloids, ce diffusion, osmosis, transport) that are important for physiological Process 8 Intended Learning Outcomes (ILOs): At the end of the course the students will be able to- 1. Explain the fundamentals of cell biology. 2. Describe the specialized structures of eukaryotic cell. 3, Explain the components of cytoskeleton. 4, State about cell cycle and cellular components underlying mitosis and meiosis cell division highlighting similarities and differences. 5, Explain the biophysical phenomena of cell and their importance. Course Content: . eee: Historical background of cell biology and cytology, nee a a and living process; the identifying characteristics of 7 ig a zu aes ae and structure of prokaryotic and ukary‘ and their differenc . ques af ay call fractionation ces, cell theory; modern techniques . Cell Membrane: Physical and ie ae Physical and chemical structure of cell membrane ion; membrane models: Danielli-Davson, Rober'so” Nicholson-Sin, e nace membranes, get models, lipid bilayer and functions of cell - Ultra Structure and Fun and function of cellular bodies, end , endoplasmic ret; chloroplast, Plasmic retj v 3, ction of Eukaryotic Cells: Details structu® organelles: mitochondria, nucleus, £° gs culum, lysosome, ribosome, microbodies an *. Hons.) curri cul Mum for the session 2021-2022 and 2022-2023 :Department of Genetic Engineering and Biotechnology, CU 4. Physicochemical Structure of Chromosomes: Definition and composition of chromatin, nucleosome shape and organization, physicochemical characters of histone and non-histone protein and their importance in DNA compaction. 5. Cytoskeletal Filament and its Regulation: Definition, structure, function and classification, nucleation, emanation and filament elongation of microtubules, intermediate filaments and microfilaments, protein binds with filaments, protein interact with filament ends. 6. Cell Division: Cell cycle, cellular senescence and their regulation, mitosis, meiosis and reproductive cycle, and their biological significance. 7. Biophysical Phenomena of Cells: Colloids, permeability, diffusion, ‘osmosis, active and passive transport, energy harvest, conservation and regeneration. References: 1, Molecular Biology of the Cell: Bruce Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, J. D. Watson. 2. Principles of Biochemistry: Lehninger, A.L. 3. Molecular Biology: Arora, M. ‘ 4, Cell and Molecular Biology: De Robertis, E.D.P. and De Robertis, EMF. 5, Plant Cell Structure and Metabolism: Hall, J.L., Flowers, T.J. and Roberts, R.M. 6. Cytology: Verma, P.S. and Agarwal, V.K 7. Cell Biology: Verma, P.S. 8. Campbell Biology: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson Teaching Strategy: Lecture: The major way of instruction is the interactive lecture. Teacher can use different teaching aids including audio-visual. systems to make classes understandable and lively. Assessment Strategy: 1. Interim Assessment: Maximum two/three assessments shall be conducted for half or full course respectively, which is known as class test. The date and topic will be scheduled and announced by the respective course teacher(s). . Final Examination: Will be conducted as scheduled by the controller of examination of CU on full course content (total 50/75 marks). Students will have to answer at least five out of eight questions. 3. Examination of Answer Script: Answer scripts will be examined by two examiners following double blind evaluation system and difference of above 15% marks will be third examined. B. Sc. (Hons.) curriculum for the session 2021-2022 and 2022-2023 15 nDepartment of Genetic Engineering and Biotechnology, CU Course: GEB. 103: Geneties and Cytogenetics Marks: Credit Hour: Lecture No, 50 02 30 Rationale: This course is designed to and alteration of the genetics an functions related to inheritance. learn transmission, distribution, arrangement d cytogenetics information and their Course Objectives: Genetics e Familiarize with the principles of inheritance formulated by Mendel's laws Understand the fundamentals and applications of genetics and cytogenetics Acquire knowledge about genetic interaction Develop interest on population genetics research Acquaint with statistical knowledge to interpret and analyze biological data Cytogenetics Understand the cytogenetics based on chromosomal aberration Know the mechanism of sex determination and maternal inheritance Intended Learning Outcomes (ILOs): At the end of the course the students will be able to- Genetics 1. Elucidate genetics including Mendel's law, deviations, linkage, recombination and crossing over. 2. Apply the principles of Mendelian inheritance and their exceptions including multiple allelism, lethal alleles and gene interactions. 3. Explain the methods of polygenic inheritance. ei fualyze and interpret genetic data using statistical approaches. . pp 2 Bere ee Jew to analyze population genetics for sex > WOrium gene, Cytogenetics gene, and heterozygote frequency. 1. eee noma chromosome number, structure, and behaviour in y caus i ions i minber sadioe at and effect of its alterations in chromosome 2. Outline the cytogenetic basi i ; Outlin asis of inheri inheritance with maternal effects. oe Course Content: Genetics: 1. Mendelism: M * Mendel' . . Mendel's laws, el's lawsycauses of his success, limitations of BS (a) Gee (Hons,) curriculum for the session 2021-2022 and T020-D003 6Department of Genetic Engineering and Biotechnology, CU 2. Linkage and Crossing Over: Definition, theories, poem reer" 3. Gene Mapping: Genetic recombination among linked genes, gene mapping in see -_" gene sequences in chromosome, 4, Alleles: Allelism, normal alleles. 5. Interaction of Genes complementary, epistasis and other kinds of gene interactions. ultiple factor hypothesis, polygenic inheritance. 7. Population Genetics: Hurdy-Weinberg principles, Hurdy-Weinberg equilibrium, ‘calculating allelic frequency,/assumiption of Hurdy- Weinberg equilibrium, proof of Hurdy-Weinberg. equilibrium, generation time, testing for fit to Hurdy-Weinberg equilibrium, extension of Hurdy-Weinberg equilibrium. Cytogenetics: 1. Chromosomal ‘Aberrations: (i) Numerical aberrations: Aneuploidy, euploidy, polyploidy and (ii) Structural aberrations: deletion, duplication, inversion and translocation; roles of chromosomal aberrations in evolution. 2. Cytogenetic Basis of) Sex»Determination: Discovery of sex chromosomes, chromosomal basis of sex determination in different organisms, sex-linked inheritance, sex influence and sex limited characters, cytoplasmic inheritance; maternal effects and inheritance. References 1. Genetics: Strickberger, M.W. 2. Principles of Genetics: Gardner, Simmons and Snustad. 3. Fundamentals of Genetics: Islam, A.S. 4. Principles of Genetics: Enmund W. Sinnott. 5. Genetics: Gupta. 6. Cytogenetics: Swanson, C.P., Merg, T. and Wang, W.J. 7. Quantitative Inheritance: David Suzuki. 8. Principles of Genetics: Tamarin, R. B. Se. (Hone ) eurrienium for the session 2021-2022 and 2022-2023 17logy, CU Department of Genetic Engineering and Biotechno. ing Strategy: | : : fees The ial way of instruction is the interactive lecture. Teacher can use different teaching aids including audio-visual systems to make classes understandable and lively. Assessment Strategy: 1, Interim Assessment: Maximum two/three assessments shall be conducted for half or full course respectively, which is known as class test. The date and topic will be scheduled and announced by the respective course teacher(s). 2, Final Examination: Will be conducted as scheduled by the controller of examination of CU on full course content (total 50/75 marks). Students will have to answer at least five out of eight questions. 3. Examination of Answer Script: Answer scripts will be examined by two examiners following double blind evaluation system and difference of above 15% marks will be third examined.Department of Genetic Engineering and Biotechnology, CU Course: GEB. 104: Basic Biochemistry Marks: Credit Hour: Lecture No, , 3 083 43 Rationale: : This course is designed to provide fundamental concepts of different biomolecules and their structure, properties and biological importance, Course Objectives: © Understand the basic concepts of biomolecules and their biological functions Conceptualize the classification, structure, function and physicochemical properties of carbohydrates Understand the classification, characteristics of fats, fatty acids and sterol, structure and function of different classes of lipids, isolation of cholesterol e Acquire knowledge on physical and chemical properties of amino acids, peptides and proteins Understand the determinants of various protein structures © Acquaint with vitamins and their biological importance Intended Learning Outcomes (ILOs): At the end of the course the students will be able to- 1. Define, classify and describe the biological roles of different biomolecules. 2. Explain the structure, function and Properties of several types of carbohydrates, 3. Describe the classification and characteristics of fats, fatty acids and Sterol, structure and importance of different lipids, isolation of cholesterol. 4. Define and classify amino acids and proteins, 5. Describe physicochemical Properties of amino acids and proteins along with their role in different levels of protein structure, 6. Describe classification and biological roles of vitamins and health concerns, Course Content: 1. Biomolecules: Definition, classification and biological importance of _ bio-molecules. B.Sc, (Hons.) curriculum for the session 2021-2022 and 2022-2023 20Department of Genetic Engineering and Biotechnology, CU 2. Water: Structure, properties and role of water in the life process. 3. Carbohydrates: Occurrence, nomenclature, classification, biological importance and chemical characteristics of carbohydrates. (a) Monosaccharides: Nomenclature, optical and chemical properties, ring structure of common monosaccharides, proof of ring structure of glucose, mutarotation, general properties and color test of reducing sugars, important derivatives of monosaccharides, and sugar acids. (b) Disaccharides: Maltose, lactose, sucrose and other disaccharides. (©) Polysaccharides: Storage and structural polysaccharides, occurrence, structures and properties of starch, glycogen, cellulose, other polysaccharides of biological interest, their chemical tests and biological functions. 4. Lipids: Definition, nomenclature, classification, general reaction of fats, fatty acids and sterol, structure and biological importance of different classes of lipids, isolation of cholesterol from natural sources. 5. Amino Acids and Peptides: Definition, classification, structural features, physicochemical and cids and peptides, essential and nonessential amino acids, concept of residue, peptide bonds, oligo-peptide and polypeptide, identification of N- terminal and C-terminal residue of peptide, analysis of amino acids from protein hydrolysate. 6. Proteins: Definition, classification based on chemical nature/structure, shape-size and biological functions, primary, secondary, tertiary and quaternary structure of proteins, protein domain and subunit, it of proteins, isolation of protein. 7. Vitamins: Definition, nomenclature and classification of vitamins, daily requirements, biological functions and their deficiency syndrome. References: 1. Lehninger Principle of Biochemistry: David L. Nelson, Michael M. Cox 2. Herper's Illustrated Biochemistry: Robert K. Murray 3. Biochemistry: Jeremy M.-Berg and Lubert Stryer 4. Fundamentals of Biochemistry: Jain J.L., Jain S., Jain NS Chand & Co Ltd., India. 5. Biochemistry: Satyanarayana 6. Textbook of Biotechnology: E.S. West, W.R. Todd, H.S. Mason and J.T. Van 7. A Textbook of Organic Chemistry: Arun Bahl and B.S. Bahl B. Se. Hons.) curriculum for the session 2021-2022 and 2022-2023 21Department of Genetic Engineering and Biotechnology, 7 »CU Teaching Strate! Lecture: The major teractive Jo, can use different teaching aids including audio-y; classes understandable and lively. O-Visual gyeq UT Ystems 4. hy 0 tpt Mae Assessment Strategy: 1. Interim Assessment: Maximum two or th TEC agge, SSessm, conducted for half or full cour: i test. The date and topic MHilibS ekebutey which ig ie Sha ; ea course teacher(s) before one veeel and annou as aS , of Se ret ail ducted as bs bs maby thy : course c ES wmetin tle two examiners follo i Srp h -Anawer stripts wil pets 7 wing double blind made © examineg difference of above 1 5% ‘ 6 marks will be third euisined System, a Rip eo oe (ons. curious ane aring and. Biotechnology, CU ic Enginee of Genel : Deparime) a? sie Microb logy es = Lectin : GEB. ; Course: W Rationale: igned to provide fundamental concepts of microbe This course IS desig! oy and its scope jectives: : ii Course a asic microbiology and its scope / ¢ Concep' ledge on different classes of micro-organisms includ e Acquire pace fungi and actinomycetes m bacteria, viruses, gement of microbial contamination id the mana i : ; tanaie knowledge on microbial diseases and their prevention e Intended Learning Outcomes (ILOs): ‘At the end of the course the students will be able to- 1. Describe the historical development and application of microbiology 2. Explain the characteristics, classification, reproduction and cultivation of bacteria. 3. Explain the characteristics of plant and animal viruses, and life cycle of bacteriophages. 4. Discuss about the characteristics and reproduction of fungi and actinomycetes. 5. Discuss physical and chemical agents require for the control of a oe and relation between microbes and immune ms, ‘ og ae transmission and prevention of common infectious diseases -8, cholera, diarrhea, AIDS, Course Content: 1. Introduction and Microbiolo; 5 cope of Microbiology: Historical developmet of ” acvent of microscopy, development of i ‘PY, general features of rokaryotic and eukaryotic CA : ; aes mycoplasma, by ttsia, ae iological and f the mi 2. Viruses: acteria, algae, industrial : | morpholoey ‘omPonent plication: lytic eycle and iysoseni® =) 2 B.Sc, 'c. Hons.) Curriculum for ind the session 2021-2022 and 2022-2023Department of Genetic Engineering and Biotechnology, CU 3. Bacteria: Morphology, size, shape and arrangement of bacterial cell, flagella, pilli capsules, sheaths, cell. wall structure, cytoplasmic membrane, cytoplasm and nuclear mat nutritional requirements and nutritional types of bacteria, bacteriological media, physical condition required for growth, normal ‘ of bacteria, membrane filter count, isolation dentification of bacteria, methods of maintenance and preservation of bacteria-peri sfer, preservation with mineral oil, freeze-drying and ec. SS ie.. shape, reproduction, distribution, classification and importance. 5. Fungi: Molds and Yeast, distinguishing characteristics, cultivation, some fungi o! ial interest. Control of microorganisms by physical al agents; characteristics of an ideal anti-microbial chemical agent, major groups of anti-microbial agents, the evaluation of anti-microbial agents. 7. : Natural resistance, pathogenecity and virulence, microbial toxins, transmission and preservation of common infectious diseases: e.g. cholera, tuberculosis, tetanus and AIDS. References: 1. Biology of Microorganisms: Brock, T. D 2. Microbiology: Pelzer, M. J., Kreig, C.N.R. 3. General Microbiology: Schlegel, G. 4, Microbiology: An Introduction: Tortora, Fanke and Case. 5. A textbook of Fungi, Bacteria and Viruses: Dube, H.C. B. Sc. (Hons.) curriculum for the session 2021-2022 and 2022-2023 25™ Deparment of Genetic Engineering and Biotechnology, CU Teaching Strategy: Lecture: The major way of instructi mn is the interactive lectur can use different teaching aids including audio-visual Systeme, Teachey classes understandable and lively. IS to Make Assessment Strategy: 1. Interim Assessment: Maximum two/three assessments gh Shal] conducted for half or full course respectively, which is kno test. The date and topic will be scheduled and annow wn &S Clags respective course teacher(s). inced by the 2. Final Examination: Will be conducted as scheduled by the of examination of CU on full course content (total 5075 wile Students will have to answer at least five out of eight questi marks), 3. Examination of Answer Script: Answer scripts will be Sane two examiners following double blind evaluation s, lag 7 difference of above 15% marks will be third examined." 26 (Ho. urriculs eMltm for the session 2021-2022 and 2022-2023ering and BiotechMolOR cu pw Engin! if Gene department 6 Marks: Credit Hour: Leg 8 106 8 Biology 5 0 nth Cours tional? soned 10 rovide modern concepts in origin of y, Rationale js desiened of plants for welfare of Tet, js cours i : a ies and functional aspect i nature- aN ang ives? oo ie a of earth, life as well as plants ee d the diversities in plant ‘kingdom role of plants 10 nature jants ‘Acquire know! system and biodiversity ° . e Understand plant diseases and control of it Outcomes (ILOs): At the end of the course the-students will be able to- 1. Describe the modern concepts of the origin of life on earth. 2, Explain the diversities of plant kingdom and the causes behind it. es and reproductions of plants. 3. Describe systematics, tissuc 4, Understand biodiversity, ecosystem, its importance and means of conservation. 5. Recognize functional properties of pl utilizations. 6. Identify diseases of plants and its control measures, biotechnological implications. Intended Learning ants and its biotechnological and Course Content: LE i : i en of Cell: Evolution of anaerobic to aerobic cell, prokaryote as one re origin and evolution to modern plants and . Systematic 7 of organic evolution), biochemical evolution. iylegeatie assifications: Definition, artificial, natural an 3, Kinga ee, nomenclature of flora and fauna. and economic sacane morphology, reproduction, classification portance: (a) Prokaryotes, (b) Algae, (¢) Fungi, Lichens, (e) B Angiosperm, ryophytes, (f) Pteridophytes, (g) Gymnosperms and n B.Sc. (Hons.) curri ‘wriculum for the session 2021-2022 and 2022-2023Department of Genetic Engineering and Biotechnology, CU 4, Tissues: Origin, types of tissue, distribution, structure and functions of plant tissue systems. 5, Ecosystem: Definition, structure and function of ecosystem, (a) Ecological features of hydrophytes, mesophytes, xerophytes and halophytes (mangrove), (b) Fresh water ecology, food chain and food webs. 6. Embryology: Microsporogenesis, microgametogenesis, megasporogenesis, megagametogenesis, types of embryo sac and their development, pollination and fertilization, appomixis, endosperm, embryo, production of seeds and fruits. 7, Economic Importance of Biology: Introduction, harmful and beneficial insects, concept of insect pests; spiders and mites; concept of IPM with comparative merits and demerits, definitions of bio- agents, predator, parasitoid and vectors; criterion for good bio-agents, predaceous, parasitic and disease transmitting insects and spiders; means and ways of utilizing biological agents in biological control of pests; conservation and augmentation of bio-agents; ethnobotany. 8. Biodiversity: Definition, aims and objectives, different types of biodiversity and ecosystem stability, threatened biodiversity, causes of loss of biodiversity, need and methods of biodiversity conservation: elementary knowledge on the pattern of biodiversity in Bangladesh, indigenous, exotic, common, rare, threatened and endangered species of Bangladesh, basic knowledge on biodiversity resources of Bangladesh, IUCN and CITES, UNEP, WWF, biodiversity and biotechnology. 9. Diseases: Definition, classification and factors of diseases, utilization of bio-agents or their products in disease control, diseases caused by Protozoa, Helminthes, Mollusks, Arthropods, Chordates and some important plant diseases as late blight of potato, etc. Books Recommended: 1. Evolution: Monroe W. Strickberger. Jones and Bartlett Pub. Sudbury, Massachusetts : 2, An Introduction to Embryology of Angio: 3 i 3. Plant Anatomy: Esau, K. ee 4. A Text Book of Botany: Pandy, S.N. 5. Modern Plant Taxonomy: Subrahmanyam, NS. ) Ons) curriculum for the session 2021-2022 and 2022-2023 29Department of Genetic Engineering and Biotechnology, CU ‘N ology: Kumar, H. 7.An advanced Text Book of Biodiversi 8. Plant Taxonomy: Sharma, OP. 9. General Zoology: Storer, T.1. 10, A Text Book of Biology: Bhuiyan, A.H. and Tun, R.W, 6. General Ec ishnamurthy, i Teaching Strategy: Lecture: The major way of instruction is the interactive | lectury can use different teaching aids including audio-visual asses understandable and lively. System: ©. Teache, 7 S 0 Make ‘Assessment Strategy? 4, Interim ‘Assessment: Maximum two/three assessment: sments shall all be conducted for half or full course respectivel: ich i: test The date and topic will be veheduled braid 35 clas, ive course teacher(s). ounced by the 2. Final Examination: Will be conducted as sche of examination of CU on full course on Students will have to answer at least five out of eight questi ats 3. Examination of Answer Script: Answer scripts will be ae two examiners following double blind evaluation ee de eee B.Sc. (Hons) curri 3 *) curriculum for the for the session 2021-20, i -2022 and 2022-2023nt of Gem Departmer 7 ment a H ar ee: Leis : GEB. 107+ Course! 4 Calculus i fics ali Mathematics # , : \ d Rationale: esigned t0 provide fundamental Koei Ee iological science. bir nats relevant to biological mat! hem Course Object damental theories of mathematical Problems : Concept in approaching @ mathematical problem oF tory and solve problems related to differentia 7 ‘Underst : : integral calculus Intended Learning Outcomes (ILOs): curve. . “i a nd ie course the students will be able to- : 1. Evaluate limits by multiple methods and use it to derive the definition and "rules for differentiation and integration, to evaluate the Tate of change, 2. Explain calculus, algebra and differential equations and make ; relationship between multiple variables in the biological context, 3. Evaluate basic integrals, construct anti derivatives directly by using the method of substitution, integration by parts and evaluate definite integrals. 4. Estimate the area under a curve. Course Content: Elementary Mathematics; 1. Algebra: Quadratic functions, graphical presentation of quadratic finetions and quadratic equations, simultaneous equations and lines and quadratic inequalities, Matrices and determinants, rank of ¢ matrix, matrix inversion method, Systems of linear equations, . Paria tule, consistency ofa linear systems of equations. r bitonital g and Series: Logarithmic series, binomial expansion * 3. Vuscl, Series, remainder theorem, A Tepresi a sumation and its composition, inverse functions, geoph on ion of function and inverse functions. The modul! function, Tris : ‘, ~ “Tgonometric, 4 ‘ functio™ ©xPonential growth an st des ae Ponential and logarithm B Se. Hons.) curr 5 s,) curriculum for the session 2021-2022 and 2022-2023Department of Genetic Engineering and Biotechnology, CU 4, Co-ordinate Geometry: Equation of straight lines, conditions for two straight lines to be parallel or perpendicular to each other. Co-ordinate geometry of the circle. Cartesian and parametric equations of curves and inter-conversion of the two forms. Equations for ellipse, parabola, hyperbola, sphere and cone. 5. Vectors: Vector algebra, Dotand) Cross products; Matrices; Algebra of Matrices, Determinants, Transpose and inverse of matrices, Cramer tule, Diagonalization of Matrices and Characteristic roots. 6. Set Theory: Sets, Types of Sets, Subsets, Complement of Sets, union and Intersection of Sets, Difference of Sets, Demorgan's Law, Cartesian product of Sets, Permutation and Combination set elements. Calculus: 1. Limit, functions of a single variable and its domain and ranges. 2. Techniques of finding derivatives of functions of single variable, geometrical interpretation of derivatives. 3. Integration of functions of single variable, techniques of integration by standard formula, method of substitution, integration by parts, definite integrals and application for finding areas. 4. Test for convergence. 5. Linear and nonlinear first order ordinary differential equations (ODE), higher order ODEs with constant coefficients, solution of simple ODEs. References: 1, A text book on Differential Calculus: Mohammad, Bhattacharjee and Latif. . 2. Calculus: Anton, Bivens and Davis (Wiley and Sons) 3. Differential Calculus: Das and Mukharjee, 4. Integral Calculus: Das and Mukharjee, 5. A Text Book on Higher Algebra: Shahidullah and Bhattacharjee. 6. Higher Algebra: Bernard and Child. 7. Calculus and analytic geometry: Shenk, 2nd Edition. Fae ET Vea B. Se. (Hons.) curriculum for the session 2021-2022 and 2022-2023 33En neering, and BIOlecrnnuwyyy VU chin} strategy? i . Teaching he major way of instruction is the interactive le different te2© ing aids including audio-visual s cture, ‘y, ‘ystems ty Thal, rstandable and lively. e Strategy* essment: Maximum two/three a: ssessm: ents gl Clas, half or full co ic will be scheduled ai ive course teacher(s). nd announced by i and topic Wl respecti 8). 2, Final Examination: Will be conducted as sch amit edul of examination of on full course content aa the Control 3 aed will have to answer at least five out of eight ou) 5 mat, : . a of Answer Script: Answer scripts eae examiners following double blind evaluition examined by system a nd difference of above 15% marks will be third examined. urse respectively, which is known hall be as B.Sc. (He fons.) curric ulur im for the session 2021-202: -2022 and 2022-2023ingineering and Biotechnology, CU Department of Genetic Ei Course: GEB. 108: Biophysical ria Cre tet Chemistry Rationale: d to elucidate various phenomena of biological This course is designed to : systems in terms of biophysical chemistry. Course Objectives: anic Chemistry _ Un their interactions in molecules Understand the atomic structure and n e Know different chemical bonding, valency, mechanism of redox reaction and redox potential , Acquaint with molar theories of acid-base and different aspect of radiochemistry Physical Chemistry : Understand the different laws of thermodynamics and their application in biological reactions © Conceptualize solution properties of molecules, osmotic pressure, adsorption, colloids and their relation with biological system Familiarize reaction equilibrium and electrochemistry and their application in biology Intended Learning Outcomes (ILOs): At the end of the course the students will be able to- Inorganic Chemistry 1. Understanding the basic properties of atoms, and different models of atomic structure, 2. Explain chemical nature of different types of bonding, relation with valency and mechanism of redox reaction. 3. Describe modern theories of acid-base and concept of radiochemistry and its usages, Physical Chemistry 1. Explain laws of thermod: i i i i lynamics and the’ ion in bi i Be ir application in biological B.Sc. (Hons.) curriculum for the session 2021-2022 and 2020-2023 36Department of Genetic Engineering and Biotechnology, CU 5. Describe colligative properties of solutions and their significance in biological system. 6. Explain chemical equilibrium, equilibrium constant and relation to free energy changes; electrochemistry and their application in biology. Course Content: Inorganic Chemistry: 4, Atomic Structure: Modern concept of atom, Rutherford and Bohr's atom model, quantum numbers, electronic configuration of elements, __ Saimin haanoaions 2. Valency: Elementary ideas about valency and different bond types, electrovalence, convalence, shape of a co-ordinate convalence, shape 3. tion: Classical and modern concept, oxidation state and number, equivalent weight of oxidizing and reducing substances in reactions, red ox potential. 4, Modern views about Acids and Bases: Theories, their applications and limitations, strength of acids. 5. Radiochemistry: Concept of radiochemistry, radioactivity, half-life, mean-life, decay constant, artificial radioactivity and uses: Physical Chemistry: 1. Thermodynamics: Work, energy and leaf dynamics, first and second law of thermodynamics, internal energy, entropy; enthalpy, laws of thermo chemistry, heats» of reaction, reversible and irreversible process, entropy and free energy. 2. Colligative Properties: Osmotic pressure, vapour pressure, boiling point and freezing point adsorption, colloids, classification, purification, properties and importance. 3. Chemical Equilibrium: Nature of chemical equilibrium, equilibrium constant, relationship to free energy, and application in biochemistry 4. Electrochemistry: Electrolytic. dissociation, ionic ‘migration and transport number, ionic and hydrolysis of salts, measurements of pH, buffers, and indicators- B. Se. (Hons.) curriculum for the'session 2021-2022 and 2022-2023 37jnology. &2 Department of Genes Engine’ gana Biotechnology P.W. References: | LA General Chemistry: Atkins, 2. Chemistry: Bahl, B.S. ee aero 3. Physical Chemistry . Raymond Chang. with Application oO! struction is the interactive lecture. Teacher o-visual systems to make Teaching Strategy: . y of in ; 5 including audi Lecture: The major Wa} i can use di rent teaching aid: classes understandable and lively. wo/three assessments shall be tively, which is known as duled and announced by the Assessment Strategy? 1. Interim Assessment: Maximum 1 conducted for half or full course respet st. The date and topic will be sche class tes respective course teacher(s). 2, Final Examination: Will be conducted as scheduled by the controller urse content (total 50/75 marks). ff CU on full cot at least five out of eight questions. 3. Examination of Answer Script: Answer scripts will be examined by two examiners following double blind evaluation system and difference of above 15% marks will be third examined. of examination ©: Students will have to answet B. Sc. (Hons.) curric .) curriculum for the sessio n 2021-2022 and 2022-2023ag and Biotechnology, CU ‘Genet Engineering sartment of Gene Dep EB. 109: Funda dT mentals Course: GI of Computer ant damental knowledge in Compu, Rationale: d also aims of understanding _ This course is science and infor computer programing. .d to provide fun igne' desig) fogy & formation techno! Course Objectives: Conceptualize hardwa Familiarize the ways ! ¢ PowerPoint presentation. and software management practices. are i omputer networking. in approaching © Intended Learning Outcomes (ILOs): ‘After the end of the course the students will be able to- 1. Understanding the working principles of computers and their applications. 2, Introducing Hardware and Software of computer. 3. Understanding and applying computer programming; a practical perspective. 4, Basic understanding of computer maintenance. 5. Understand how to prepare slides for PowerPoint presentation. Course Content: 1. Introduction: Types of computer, application areas, keyboard, mouse, other input/output devices, hard disk and the like peripheral, working principles of computer systems, overview of hardware and software, Windows. Unix/ Linux. 2. Number System and Logic Circuits: Number system; binary, octal, decimal, hexadecimal, binary addition and subtraction, coding, ASCII, EBCDIC, BCD, Unicode, Boolean algebra, Logic operation gates, functional gates, universal gates, simplification functions, Boolean postulates and laws, Logic circuits. , . ores Sheet Analysis: Mathematical and Statistical Function: ‘requency, standard deviation, variance, mean, median, line, bar, p!® graph, correlation, regression, etc., creating and formatting chatt, printing sheet, problem solving using formulas, data consolidation. w B.Sc. curr (Hons.) curriculum for the session 2021-2022 and 2022-2023 40 \Department of Genetic Engineering and Biotechnology, CU 4, Power Point Presentation: Preparation of slides, tables, graphs, editing, copying. 5. Database: (i) Concept of field, record, table, database and database management system. (ii) Creatiiglaid adding information to a database. (iii) Introduction to flat files, DBMS and RDBMS, ER “model. 6. Computer Networks: Classification of Computer networks, Network _ topology; World Wide: Web (WWW), Basic concepts of networks, , Network security: basic concepts of public key and private key cryptography, digital signature firewalls, Application layer protocols (icmp, dns, smtp, pop, ftp, http). 7. Programming Language and Algorithm: Programming languages, algorithms and flow charts. Introduction of C and C++ with fundamental object-oriented concepts: and constants, operators, i ements, ms, arrays. References: 1, Fundamentals of Computers: Rajaraman,V. 1999: gid Ed, Prentice- Hall of India, New Delhi. 2. Teach Yourself Microsoft Office 2000: Grey Perry, SAMS:, Tec media. 3. Troubleshooting Your Multimedia PC: Montgomery,J. Addison- ‘Wesley Publishing Company, NY, Singapore. 4, Introduction to Computers: Norton, P. B. Sc. (Hons.) curriculum for the session 2021-2022 and 2022-2023 41; zu ering and Biotechnology, CU Department of ' ‘Genetic Engin Teaching Strategy: ae i Lecture: The major way of instruction is the interactive lecture, Te, can use different teaching aids including audio-visual systems to classes understandable and lively. Ache, Make Assessment Strategy: 1. Interim Assessment: conducted for half or fu test. The date and topic will be respective course teacher(s). 2, Final Examination: Will be conducted as scheduled by the controle 1 of examination of CU on full course content (total 50/75 marks Students will have to answer at least five out of eight questions. , 3, Examination of Answer Script: Answer scripts will be examined by two examiners following double blind evaluation system ne difference of above 15% marks will be third examined. Maximum two/three assessments shall 4, 11 course respectively, which is known as ¢} : scheduled and announced by ia le B. Sc. Hons.) curriculum for the session 2021-2022 and 2022-2023Department of Genetic Engineering and Biotechnology, CU Major Practical Courses GEB. 110 : Introductory Genetic Engineering and Biotechnology; Cell Biology and Cytology; Genetics and Cytogenetics A. Introductory Genetic Engineering and Biotechnology 1. Preparation of stock solutions 2. Techniques of media preparation 3. Sterilization techniques of media instrument and explants. B. Cell Biology and Cytology 1. Cell division: Different stages of Mitosis and Meiosis. 2. Karyotypes and Idiogram studies. C. Genetics and Cytogenetics 1. Determination of gene sequence in chromosome and construction of genetic map from triple test cross data. 2. Study of genetic interactions. 3. Induction of polyploidy. 4. PTC test for identification of presence/ absence of taster gene. GEB. 111: Basic Biochemistry and Basic Microbiology A. Basic Biochemistry 1. Preparing aqueous molar solution. 2. Using analytical balance, identifying volumetric and non-volumetric equipment. 3, Preparing stock solution, standard solution and standard curve analysis. 4. Qualitative test for Carbohydrate, Protein and Lipid. 5. Estimation of vitamin from supplied sample. 6. Determination of saponification value of fat or oil. 7. Separation of proteins by "Salting In and Salting out" technique. B. Basic Microbiology 1. Study of different types of Microscope and their handlings 2. Microscopy, fixative, stains solution used in cytology. 3. Simple, Gram and Spore staining techniques 4. Slant and Strick culture 5. Total viable count by Pour plate technique 6. Study of selected organisms- E. Coli and Salmonella B. Sc. (Hons.) curriculum. for the session 2021-2022 and 2022-2023 43a jecring @ iote , CU 1 of Genetic Engineering, ind Biot chnology, , 112: Basic Biology oe ay of fresh water 4 |gac. lichen. ; i Sa study Marchantia and Selaginella. inus with cones. . 4, ae of eal ad anatomical brace features of selected 7 varophytes xerophytes, epiphytes and halophytes. 6. Bthnobo itudi d radial including roots, stems jioning: Transverse, longitudinal and ra 2 Sectioning icotyledonous and montocotyledonous plate 8. Study of Microsporangium and microspore tetrad ani ‘ospores : (both simple and compound) of different ea 9. Study of ‘megasporangium and types of ovul es 10. Study of representative specimens of major ¢ asses. 11. Dissection of major system: Prawn, Pila and any Fish. 12. Laboratory culture: Culture and study of Ciliates, Hydra and Drosophila. 13. Study of permanen' slides. Departmen t slides: Zooplankton, parasites, histological GEB. 113: Biophysical Chemistry and F ‘undamentals of Computer and IT A. Biophysical Chemistry 1. Qualitative analysis: Lead, Copper, Aluminium, Zinc, Manganese, Calcium, Magnesium, Sodium, Potassium, Chloride, Sulphate, Carbonate, Nitrate and Borate 2. Use of analytical balance 3. Preparation of standard solution 4, Standardization of HCl and NaOH 5. Estimation of copper by iodometry method 6. Estimation of iron content of Mohr's salt by dichromate method 7, Estimation of calcium by permanganate method 8. Determination of PH of a buffer solution oe of Computer and IT . Spreadsheet analysis, database, statistical analysis of Li id their graphical presentation and additional tical based catcatiee : it 2. Preparing PowerPoint slides. Miele tae 3. Calculation in MS Excel. 4. Typing and formatting in MS word 5. Developing a programming language C+, MATHLAB B. Sc. (Hons.) curriculum for the session 2021-2022 and 2022-2023 44