25/06 18. | EXPERIMENT-5 a TEST FOR CAR BOHYDRATES To study some Simple tests ol careobyd rater REQUIREMENTS : Glucose ,Sucitse , lactose , starch , Molisch's reagent , Felniimg’s sol. ,Genedict’s sol, and Triien's vreagem> PROCEDURE : 1. Motisel Test — LAM Carteolhydaat: vives Hhis teak. artboly; et give: this tea Take \-amt of aq. sol. db carvbchydeates and add jeu chreps of, Molisch’s weagent. Pub one mio} conc. 4450, slowly falong. the side olf, the test tube . > Ared viclet ring Ree ot the junction of tes layers » Chemistry ob-the bert. a — essere Conc. 550), Comvests cosbclydeates Tmo. Fuslwral ow its jlo vativer calbic Yurrtbey reacts usith \- napltlne) to Give q coloured product. | 2. [Febting’ s Test | Talce 2-mt ofagscl.ol, coctEclagdeote and add amt each | oreehine Ss Sol. A and Febling? s spl. a. Keep the test tube in. boiling wakey bath . | Reddish ppe- indicate the Presence of a ~educing sugar || Chemistry OL the test. | cho C07 Nat | i t | Conondy + 2 Cu (OH), + Naor TEZESHE SCHON) + ato + COL ions vm! e | rred. | CHAOK CHOH ie | | | Glucose sod. satt of } - (ucomic acid t I qluconic ad ' 1 — ~ ~ t 3, Bene ’s Test 7 2 | [Te tami cLag. sol. ol, carbohydaote qm a test tube , | —bedd team ob Benedict's rreagem\-. Keep tbe fort tube — qh. .c_tesiling inate bath - | Reddish ppt indicates the presence ol, reducing suger, | { Chemistry ofthe teak. _ — Coo Nat . | Conor) y +2 Cu CoH), + Nady Strate COHOH)y + 3Hs04 CuO Tons \ te re 7 CH,0H OF =| Ca liens ge Sed-satt of qlucenicadd Tollen's Test. —;Talce_2-3 mi ol, ag, sol oh carbohydvate Tm Atel | habe Adel_to it 2-2 mi_ol, Tallen’s reagent. Keep-the teste bebe Ino boiling taker. bath her (oO minutes . | 7A shining Silver ratrron jrdicates the presence of reducing carbohy darates.Chewis: ol} the & Agnos + NAYoH —3 Ny No, 4+ AgoH 2AgOH —3 Aq, 0 +30 Ag. 0 + &NH,,oH —A 2 [Ag (Nt Jor + 3H yd (Soluble ) CH 0H COOH (chon), + Ag oPESS# > (cHom), + aR | Silver miner CHO Coon Glucose Glucnicaad RESULT: , Studied re tats |or carbobyd votes. aT