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HPLC Hemoglobin Test Report Summary

Mr. Rajdeep Mondal, a 31-year old male, underwent an HB electrophoresis (HPLC) test which found his Hb A0 level to be 85.8%, Hb A2 level to be 2.5%, and Hb F, Hb S, Hb D, Hb C, and E levels to be within normal ranges. The impression was that there was no evidence of abnormally elevated Hb variants. The report provides further information on HPLC fractions and cautions about clinical correlation.

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Paramita Bera
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55 views2 pages

HPLC Hemoglobin Test Report Summary

Mr. Rajdeep Mondal, a 31-year old male, underwent an HB electrophoresis (HPLC) test which found his Hb A0 level to be 85.8%, Hb A2 level to be 2.5%, and Hb F, Hb S, Hb D, Hb C, and E levels to be within normal ranges. The impression was that there was no evidence of abnormally elevated Hb variants. The report provides further information on HPLC fractions and cautions about clinical correlation.

Uploaded by

Paramita Bera
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Patient Name : Mr.

Rajdeep Mondal Bill Number : TB/NVM/230611/0063


Patient Age : 31 Years , Sex: Male Bill Date : 11-Jun-2023 03:19 PM
Reg. Number : P230611/28 Collection Date : 11-Jun-2023 03:20 PM
Address : 68/5 BARISHA PURBA PARA Lab Received Date: 11-Jun-2023 03:22 PM
Referred By : Dr. SELF Report Date : 13-Jun-2023 06:41 PM

TEST REPORT
HB ELECTROPHORESIS (HPLC)
TEST RESULT UNITS REFERENCE RANGE
CBC 11.5 – 16.0
14.6 g/dL
Total Hemoglobin
Total RBC Count 4.43 milli/cum 3.8 - 4.84
RDW - CV 12.9 % 11.5 - 14.5
HCT 42.0 % 36.0 - 46.0
MCV 94.7 FL 82.0 - 92.0
MCH 32.9 PG 27.0 - 32.0
MCHC 34.7 gm/dl 32.0 - 36.0

Hemoglobinopathy (by HPLC Method)


Hb A0 Level 85.8 % 94.3 – 98.5
Hb A2 Level 2.5 % < 3.5
Hb F(Foetal Hb) < 1.0
< 0.8 %
Level
Sickle cell Window 0 % 0- 0

Hb D -Window 0 % 0-0

Hb C -Window 0 % 0-0

Hb E -Window 0
Unknown Variant 0 % < 1.0

Impression : No evidence of abnormally elevated Hb variants for HbS/HbD/HbC/HbE/HbF.

VERIFIED Verified by PRADIP KHANDA at 13-06-2023 06:47 PM, Printed at 13-06-2023 09:48 PM
Page 1 of 2
Patient Name : Mr. Rajdeep Mondal Bill Number : TB/NVM/230611/0063
Patient Age : 31 Years , Sex: Male Bill Date : 11-Jun-2023 03:19 PM
Reg. Number : P230611/28 Collection Date : 11-Jun-2023 03:20 PM
Address : 68/5 BARISHA PURBA PARA Lab Received Date: 11-Jun-2023 03:22 PM
Referred By : Dr. SELF Report Date : 13-Jun-2023 06:41 PM

HPLC fractions include glycosylated haemoglobin and other non specific haemoglobin fractions which are not relavant in analysing Thalassaemia or a
Haemoglobinopathy. Thus % analysis may not add upto 100%. Please refer to the HPLC graph for a full analysis. HbE-HbA2 can not be separated by
existing HPLC techniques. By Variant-II but can be separated by D-10 (Bio-Rad).
The values of HbA2 may be decreased in associated iron deficiency anemia, thus masking a b-Thalassemia Trait.
In case clinical correlation indicates a haemoglobinopathy, a repeat estimation of HbA2 after correction of iron deficiency may help in getting a correct
diagnosis.
Mild cases of a-Thalassemia Trait (Type1 & Type2) are generally not detected by Hb-HPLC. The analysis of haemoglobinfractions should be clinically
correlated.

------End of Report------

Dr. Tathagata Adhikari Dr. Subhra Sil Dr. Abhijit Banerji


MD (PATH) MBBS, MD(PATH) MD (PATH)
Consultant Pathologist Consultant Histopathologist & Consultant Pathologist
Scan for genuinity
Cytologist

VERIFIED Verified by PRADIP KHANDA at 13-06-2023 06:47 PM, Printed at 13-06-2023 09:48 PM
Page 2 of 2

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