ss BaNeoicts_ METHOD AIM: To eskimale the _amoun\ af qlucose present Ww _ unknown __ Sample of usine by benedicts method. —>_ APPARATUS: Buretle, pipette, volumelvic Rlauk, , Porcelain chips : > REAGENTS Benedticts quantilabive reagent i Avbidnous Sodium coubonate - —>_ PRINCIPLE oO == + Curt Fheabbase 5c + Gyow) 4 Oo” tecl. Aldehjde. Caloorylic Precigilal add See Gj + Cutt heat base. Cisse o copper Tl sulfate, sodium zpolantum lhtewonate crclof reducing sugars: os + Copper Cif) sulfate px are reduced reduc * Sodium carbonete proviela basle environment, Which & hecemamy for the redox reackion to occur: + (f the carbone fons from the sodfum carbonate Were bo combine with copper (I) fons fiom the copper CM) sulphate, Insoluble coppaC) corkonedle Wouldfown |: To prevent be formation of coppe CT) carbonate precipitate , sodium cihale 6 adcled . the cilrate ions chelate with copper) fons before the copper(M)fons can_preciptiate with the carbonate Sons « The Reducing sugar recluces -the copper (If) Yong bo copper w jee ean combine with the thiccyjans fons from Are polasstun Aliccjanate bo Form while copper (1) thifocjancle+ This makes the end pon: tre Hhation, the bansition from blue fe white, tadily visible - + The sell_awount of polasdium Aeresjanide prevents ave ceoxtdantion af Atne copper Cr) ts. ie PRoce URE to ml af benedfel's reagent Was pipette out thhoa __ clean covicalfiask. About Goo st ernie see A _alkalivilygkarts 7 ene ada lucose elution dropwise unbl Ae blue cobut'discppeaud. he volume of glucose | love down from lice burelte Ps voked - , The given unknown sugar soluble was nude upho_loom| Yo slandeud flask witb dishilled water «Then the burette was Filled with Abis Unknown sugeu. solution - The benedict reagent f hated with the sugar solution uwkl dhe colour Afsappears. <> REFERENCE: Compeendiole Viva ard Paclical Bodnemishy