Precautions Store enzyme immediately to prevent it from oxidizing Extract the enzyme at a low temp (below 50degrees) 11) 55 degrees Less than 10 degrees The enzyme Is inactive 10-55 Enzyme became activated Enzyme activity increased with increasing temperature Enzyme reached its maximum activity at 55 degreesMore than $$ degrees Enzyme ts starting to denatured |, Add substances of varying ph levels to different test tubes make sure it'd same volume 2. Add the same amount of the enzyme to each test tube for the same time of period 3, Add the same Quantity of protem sample to each test tube ¢, Determine the time it takes for each protein sample to disappear 5. Record results in appropriate chart/ table