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Effect of Comorbidity-Free Neonatal Hypoglycemia On Neurodevelopment at 18 Months of Age: A Prospective Cohort Study
Effect of Comorbidity-Free Neonatal Hypoglycemia On Neurodevelopment at 18 Months of Age: A Prospective Cohort Study
Correspondence to: Objective: To study the impact of neonatal hypoglycemia on neurodevelopment and neuro-
Dr Ashfaq Masood, Sher-i-Kashmir developmental clusters at 18 months of age. Methods: This prospective cohort study was
Institute of Medical Sciences, conducted at the pediatric and neonatal wards of a tertiary care hospital. Study subjects were
neonates with hypoglycemia (blood sugar <47 mg/dL at presentation). Enrolled babies were
Srinagar, Jammu and Kashmir.
evaluated at 3,6,9,12 and 18 months for overall neurodevelopment and neurodevelopmental
dr.sayyidashfaq@gmail.com clusters by Developmental Assessment Scale for Indian Infants (DASII). Result: Of the total
Received: Dec 7, 2022; 259 neonates with hypoglycemia, 92 met the inclusion criteria, and 85 babies could be
Initial review: Dec 30, 2022; evaluated at 3,6,9,12 and 18 months. 20 (23.5%) neonates had asymptomatic hypoglycemia,
Accepted: Sep 20, 2023. and 7 (8.2%) had symptoms with seizures. 17.6% (n=15) babies had delayed development
quotient for development at 3 months of life. At 18 months of age, 9.4% (n=8) subjects had
delayed development quotient for motor clusters and 7% (n=6) had delayed development
quotient for mental clusters. Positive correlation was found between age and both improvement
in motor development (r=0.99, P<0.05) and mental development (r=0.95, P<0.05) clusters.
Conclusion: Motor and mental developmental clusters are affected by neonatal hypoglycemia.
Improvement in developmental clusters occurs with increasing age.
Keywords: Developmental delay, Follow-up, Screening, Outcome.
N
eonatal hypoglycemia is a common clinical METHODS
entity [1]. Transient hypoglycemia is common
during metabolic transition to extra uterine life This is a hospital based prospective study conducted from 1
in term neonates [2], and is unrelated to nutrition October, 2016 till 31 May, 2019 at the pediatrics and neo-
[3]. There is no universal consensus about the safe blood natology departments of one of the tertiary care centers of
sugar level for newborn infants, partly because individual Jammu Kashmir, and enrolled neonates referred from
susceptibility to brain injury varies with factors like gestatio- different maternity centers in urban and rural areas of the
nal age, birth weight, presence of comorbid conditions and region between 1 October, 2016 and 30 November, 2017. The
the ability of the infant to produce and use alternate cerebral study was approved by the institutional ethics committee. All
fuels [4]. Presently most guidelines focus to keep blood sugar term and late preterm neonates (>34 weeks gestational age)
level above 2.5 mmol/dL (>45 mg/dL) [5,6]. Multiple with weight appropriate for gestation and having documented
studies have confirmed neurodevelopmental delay associa- hypoglycemia (<47mg/dL) were included in the study. The
ted with neonatal hypoglycemia [7]. blood glucose estimation was done by bed side glucometric
strip method and then confirmed by glucose oxidase enzy-
While assessment of neurodevelopmental clusters is a matic method using fluoride containing tubes [8].
better way of assessment of individual motor and mental
development, hence studying longitudinally and analyzing We excluded those neonates that had comorbid factors
individually these clusters at different ages will give insight in that could affect neurodevelopment as cofounding factor
neurodevelopment delay. There is a paucity of literature per- viz., infants born to mothers with overt diabetes, intra-uterine
taining to impact of comorbidity-free neonatal hypoglycemia infections like TORCH, documented perinatal asphyxia,
on neurodevelopmental status. We studied the association of neonates with congenital anomaly or suspected chromo-
neonatal hypoglycemia in relation to neurodevelopment in somal abnormality, life threatening events like intracranial
comorbidity-free neonates. bleeds, severe sepsis and meningitis, hyperbilirubinemia
needing exchange transfusion or other conditions affecting Statistical analysis: The data recorded were compiled and
neurodevelopment like prematurity or low birth weight entered into a Microsoft Excel spreadsheet. Continuous
below 2500 grams. For this, antenatal, intrapartum and variables were expressed as means and categorical variables
postnatal records were analyzed. At birth, Apgar score, birth were summarized as percentages. Correlation coefficients
weight and gestation [9] were assessed and recorded. Record were calculated using Microsoft Excel version 2019. Chi-
was also made of symptomatic hypoglycemia, degree of square test was applied for comparison of various categorical
hypoglycemia, age at which hypoglycemia was observed, variables. P value less than 0.05 was considered statistically
mode of delivery, and the catchment area. Sepsis screen, significant.
metabolic abnormalities, congenital anomalies, and thyroid
RESULTS
profile were assessed.
Of the 1940 newborns referred to our unit, 259 cases were
Enrolled babies were re-evaluated at 3, 6, 9, 12 and 18 observed to have blood sugar <47 mg/dL but 167 cases were
months for overall neurodevelopment and 11 neuro- excluded because of other risk factors for neurodevelop-
developmental clusters by Developmental Assessment Scale mental injury, thereby leaving a final cohort of 92 cases for
for Indian Infants (DASII), an Indian adaptation of Bayley follow-up. Of these, data of 85 cases (without comorbid
Scale of Infant Development [10]. Neurodevelopment and factors) were analyzed, as seven babies were lost to follow-
neurodevelopmental clusters were analyzed as development up. The various demographic, clinical and biochemical
quotient (DQ). Record of development quotient in motor and parameters in relation to delayed neurodevelopment are
mental developmental clusters was recorded as: <70 shown in Table I.
(delayed), 70-79 (low optimal), 80-99 (optimal), ≥100 (high
optimal). Neuroimaging was considered in patients with At 3 months of age, 15 babies (17.65%; 95%CI 10.23%-
delayed development at any stage during follow-up. 27.43%) had delayed overall neurodevelopment (DQ<70) in
both the motor and mental domains. The rest 70 babies
(82.3%) had low optimal, optimal and higher optimal range
Table I Demographic, Clinical, Laboratory and Neuro-
of normal neurodevelopment. These 15 babies were evalua-
developmental Profile of Neonates With Comorbidity-free
Hypoglycemia Enrolled in the Study (N=85)
ted for four motor and seven mental developmental clusters.
Among motor clusters, neck control (NC), body control
Characteristics Delayed development at 18 months (BC) and locomotion skills (LS) were impaired in all, and
Motor Mental reaching and manipulation skills (RMS) in 8 (53.3%) babies.
domain domain Among mental clusters, reaching manipulation exploring
Male sex, n=45 4 (4.7) 3 (3.5) (RME), social and imitative behavior (SIB), understanding
Urban residence, n=72 7 (8.2) 5 (5.8)
surroundings (US), and memory (M) were impaired in all 15
babies with delayed overall neurodevelop-ment. Of the other
Cesarean delivery, n=64 5 (5.8) 5 (5.8)
mental clusters, visual cognizance (VC) was impaired in 9
Gestation 341-370 wk, n=23 5 (5.8) 3 (3.5) (60%), language and vocalization skill (LVS) in 8 (53.3%),
Gestation >371-420 wk, n=62 3 (3.5) 3 (3.5) and auditory cognizance (AC) in 2 (13.3%) babies (Web
Birth weight 2500-4000 g, n=71 8 (9.4) 6 (7) Table I). At 18 months of age, only 8 (9.4%) babies had
Birth weight 4001-4500 g, n=14 - - impaired motor development, and 6 (7%) babies had
Hypoglycemia impaired mental development (Web Table I).
Symptomatic, n=65 8 (9.4) 6 (7) At 3 months, Among 70 babies with no delayed overall
Asymptomatic, n=20 - - neurodevelopment (DQ ≥70), 19 cases (27.1%) had low
Symptomatic with seizures, n=7 7 (8.2) 6 (7) optimal development in all mental developmental clusters,
Blood sugar and only in RMS in motor development cluster. At 18
<30 mg/dL, n=15 1 (1.1) 1 (1.1) months, 16 babies (22.8%) had low optimal development in
30-40 mg/dL, n=37 5 (5.8) 4 (4.7) mental clusters (sparing the AC) and 7 (10%) had low
>40 mg/dL, n=36 2 (2.2) 1 (1.1) optimal development in motor.
Documentation of hypoglycemia
There was a negative correlation (r=-0.99; P=0.001) of
Age <1 h, n=1 - - age with impaired /delayed neurodevelopment in those with
Age 1-2 h, n=13 - -
delay, and a positive correlation (r =0.639; P=0.24) of age
Age 2 -6 h, n=26 2 (2.2) 1 (1.1)
Age >6 h to 5 d, n=41 4 (4.7) 3 (3.5) with number of patients with optimal development (Fig. 1).
Age >5 d, n=4 2 (2.2) 2 (2.2) Fifteen subjects underwent magnetic resonance imaging
All values in no. (%). (MRI) of the brain at 15-18 months of age; 40% (n=6) cases
had hyperintensities in the parieto-occipital region of the literature regarding neurodevelopment in neonatal hypogly-
brain. cemia in the first year of life. Two previous studies [19,20]
have shown neurodevelopmental delay in as high as 50-60%
DISCUSSION
in neonatal hypoglycemia at three months of age; however,
This study on prospective evaluation of neurodevelopment the cases in their studies had other comorbid factors that
among comorbidity-free neonatal hypoglycemic showed that could impair neurodevelopment and thus the results may not
17.8% cases had neurodevelopmental delay at third month of be comparable to our study.
life. On follow-up at 6,9,12 and 18 months, these cases (8.4%
cases in motor and 10.8% in mental) showed improvement to Although, neonatal hypoglycemic brain injury has been
sub optimal range; although, 9.4 % and 7% cases were still observed to involve predominantly the posterior portion of
having impaired motor and mental development, respec- the occipito-parietal region of the brain [11], yet no
tively. The other 82.2% cases with normal (sub optimal) consensus has been evolved for uniform diagnostic criteria in
neurodevelopment at third month of life on follow-up also neonatal hypoglycemic brain injury outcome [12]. Lucas, et
showed improvement to optimal and higher optimal range al. [21] observed that recurrent moderate hypoglycemia in
by18 months (73.4% in motor and 63.4% in mental); preterm newborns can lead to severe motor and mental
however, 8.2% and 18.8% cases in this group still had low developmental delay when followed till 18 months of age.
optimal range in motor and mental development, respectively. Annept, et al. [22], in a retrospective observational study at
Significantly delayed motor and mental clusters of develop- 6-9 years of age, observed that without risk factors the
ment in severe symptomatic hypoglycemia, especially those neurodevelopment can be normal except low optimal motor
with seizures and sustained hypoglycemia, was observed. function. Ansell, et al. [14] evaluating the neonatal hypo-
glycemia cases till 2 years observed no neurodevelopmental
Neurodevelopmental delay in neonatal hypoglycemia delay. Babies with neonatal hypoglycemia did not have any
observed in our study is a substantiation of already known major developmental delay, except low optimal develop-
facts [7,11-13]. Majority of the studies have already ment across five executive functions at 4.5 years [23].
attributed the role of comorbid risk factors in association
with neonatal hypoglycemia for brain injury and neuro- Our study has certain limitations. DASII being a clinical
developmental delay [14-16]. Some studies have also scale for neurodevelopment, visual testing like visual evoked
observed the role of associated co-factors like severe potential (VEP) and auditory function testing like oto-
symptomatic hypoglycemia and sustained hypoglycemia acoustic emissions (OAE) and brainstem evoked response
and delayed neurodevelopment [17,18]. There is scarcity of audiometry (BERA) need to be performed separately in
Web Table I Delayed Motor and Mental Developmental Clusters at Various Stages of Follow-up in Babies With Comorbidity-
free Neonatal Hypoglycemia (N=85)
Age in months Clusters of mental development Clusters of motor development
VC AC RME SIB LVS UR M NC BC RMS LS
3 9 2 15 15 8 15 15 11 15 8 15
6 8 2 11 9 8 11 11 8 13 7 13
9 5 0 8 7 8 10 10 8 12 7 8
12 5 0 6 7 6 8 8 1 8 7 8
18 5 0 6 6 6 6 6 0 8 6 8
NC:neck control; BC:body control; RMS:reaching and manipulation skills; LS:locomotion skills; VC:visual cognizance; AC:auditory cognizance;
RME:reaching manipulation and exploring; LVS:language vocalisation skills; SIB:social imitative behavior; US:understanding of surroundings;
M:memory.