1. A microorganisms is growing at its maximum specific growth rate.

Conditions are the

following

Times(Min) Cell concentration(mg/L)

0 50
1 75
2 111
3 166
4 248
5 369
a. Calculate µmax
b. What will be the population size after 25 min?
2. Consider an organsms that follows the monod growth law with max =1h-1,Ks
=0.25g.dm3 and Yc/s =0.5. The reaction is to be carried out in a CSTR with a feed
substrate concetration of 20g/dm3.
a. Calculate the dilution rate for an exiting cell concentration of 9.5 g/dm3
b. What is the dilution rate that will give the maximum product rate?
c. What is the dilution rate at which wash out occurs?
3. The bacteria X-II can be described by a simple Monod equation with max =0.8 h-1,
KS = 4 g/dm3 and YP/C=0.2 g/g and YS/C = 2.2. g/g. The process is carriedout in a
CSTR in which the feed rate is 1000 dm3/h at a substrate concentrationof 10 g/dm3.a)
What size fermentor is needed to achieve 90% conversion of the substrate. b) What is
the exiting cell concentration?c) Determine the volumetric flow rate at which wash-
out occurs and also theflow rate at which the cell production rate is a maximum?
4. The growth rate of E.coliin synthetic medium can be expressed by Monod kinetics as
where Cs is the concentration of a limiting substrate, glucose. You are going to
cultivate E.coliin a steady-state CSTF (working volume: 10 L) with a flow rate of 7
L/hr. The initial substrate concentration is 10 g/L and the cell yield constant (YX/S )is
0.6. The feed stream is sterile. a. What will be the doubling time and the division rate
of the cells in the CSTF? b. What will be the cell and substrate concentrations of the
outlet stream?