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  • Vazyme N411 Manual V21.1

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    10 views2 pages

    Vazyme N411 Manual V21.1

    Uploaded by

    thumita kumi

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 2
    VAHTS DNA Clean Beads Ww Vazyume Product Description \Vazyme VAHTS DNA Clean Beads uses SPRI (Solle-Phase Reversible Inmobilzation) paramagnetic bead technology, and applies to [DNA puriicaton and size solocton in the preparation of NGS (Next Generation Sequencing) tbrary. VAHTS DNA Clean Beads is compatible with all DNAIRNA library construction protocols currently provided by manufacturers or published in academic journals. The usage of \VAHTS DNA Clean Beads isthe same asthe same products of other suppliers, wrich is widely used in NGS brary preparation, The yield ‘and 82e astrbution ofthe Ibvaries prepared with VAHTS ONA Clean Beads are highly consistent with those with same produets of other suppliers, Components Components waiter ware waren TARTS DNAClean Baad Sint ind 0H Storage Store at 2~ 81C. Adjust the shipping method according to the destination Its apoliable for DNA or RNA lorry preparation. Self-prepared Materials Etnanol (10094) Nuclease-ttoo ddH.O and tubos Magnetic stand Notes For ressarch use only. Not for use in agnostic procedures. 1. Keep the magnetic beads at rom temperature at last 30 min and shake the reagant wll before use, therwise the recovery efcency of ‘he sample should be affected 2. When washing the sample wit 80% ethanol, keep the tube onthe magnetic stand and without cistubing the magnetic beads. The drying time should be contoled fo ensure there fs no residual ethanol and avold excessive drying (Excessive drying may cause cracking onthe surface of beads and thereby reduce the al il). 3. As shown in the gure below, when using devices based on the elecrophoret separation principle such as Agilent 2100 bioanalyzer to analyze samples, te high molecular weight tang is usualy caused by the residue of trace magnetic beads. Itis recommended fo use a ‘magnetic stand with song magnetic force inte operaton and avoid disturbing magnetic beads inte las step. o0=/0.20 oss=/0s ton WY Vezyme roi ssezssrran enabeteecmscam i: mm sanmcon oc Red Mal ech iy Pt, Nr, Experiment Process DNA Purification 1. Take out the VAHTS DNA Clean Beads from 2 ~8°C and keep the reagent at room temperature at least 30 min before use 2. Mixthe VAHTS DNA Clean Beads thoroughly by vortex or turning upside down, Add VAHTS DNA Clean Beads according tothe reaction volume in Tablo 1. Mix the VAHTS DNA Clean Beads and sample thorough¥y by pipetto mixing 10 times. 3. Incubate the tube at room temperature for 10 mint bind DNA to magnetic beads. 4. Place the tube onto the magnetic stand for about 5 min. Aer the soliton is clarified, carefully aspirate the supematant and clscarg, 5. Keep the tube on magnetic stané. Dispense 200 il of freshly prepared 80% ethanol into the tube and incubate for 30 see at oom temperature. DO NOT r 6. Repeat step 5 suspend the beads! Aspirato out the ethanol and giscard 7. Keep the tue onthe magnetic stand and uncap the tube to airy the magnetc beads for §- 10 min 8. Take out the tube from magnetic stand. Add an appropriate amount of Nuclease-tree ddH,O to the tube and manually resuspend the beads by ppeting up and down 10 times. After incubated 2 min at ream tomperature, place the tube onto magnetic stand for about Sin to separate beads from the solution, and carefuly aspirate out the supematant to a new Nuclease-tree tube. ‘Table 1, Reference condons for DNA purfiction Fragment sic ange after purest (magnate bead volans danage: ample vine) ar 05 400 to» 200m 12 2100 224.39" DNA size selection 1. Take out the VAHTS DNA Clean Beads from 2 ~ 8°C and keep the reagent at room temperature a east 30 min before use 2. Mac the VAHTS DNA Cloan Beads thoroughly by vortex or turning upside down. Add VAHTS DNA Clean Beads according tothe tst ‘ound of reaction volume in Table 2, Mix the VAHTS DNA Clean Sead and sample thoroughly ay pipette mixing 10 times. 3. Incubate the tube at room temperature fr 10 min. 4.Place the tube onto the magnetic stand for about § min, Ate the solution s clare, carefully transfer the supematant to a new Nuclease-ee tube 5. Add VAHTS DNA Clean Beads according to the 2nd round of raaction volume in Table 2 inthe new tubo, an mix the VAHTS ONA Clean Beads and sample thoroughly by pipette mixing 10 times. 6. Incubate the tube at room temperature fr 10 min, 7, Place the tube ont the magnetic stand for about 5 min, Aer the solution is clarfied, carefully agprale the supernatant and discard. 8. Koop the tube on magnetic stand. Dispense 200 ul af freshly propared 80% ctharal into the tube and incubate for 30 sec at zoom temperature. DO NOT re-suspend the Beads! Aspirate out the ethanol and discard 8. Repeat step 8 10. Koop the tube on the magnetic stand and uneap the tube ta ai-cry the magnate beads for 5 10 min, 11. Take out the tube from magnetic stand. Add an appropriate amount of Nuc ss0rae daH,O tothe tube and manually resuspend the beads by ppeting up and down 10 times. After ncubated 2 min at room temperature, place the tube onto magnetic stand fr about 5 min to separate beads from the solution, and carefully aspirate out the supernatant to a new Nuclease-fee tube, Table 2, Reference condilans for DNA size selection vara ah area 170-00 220-280 ~—<200-280~—

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