 Cytometers, Automated, Flow

Scope of this Product Comparison

This Product Comparison covers automated flow cytometers used for optical analysis and separation of cells
based on light scattering and fluorescence. Flow cytometers designed for the clinical laboratory and those
designed exclusively for research applications are included. Some flow cytometers listed in the chart include cell
sorting capabilities.
These devices are also called: cell sorters, automated reticulocyte analyzers.

Purpose
Flow cytometers allow technicians to evaluate the characteristics
of thousands of cells very rapidly. They separate, quantify, and
analyze cells by suspending them in a stream of fluid (sheathing
fluid) and passing them through a detection system. Clinical
applications of flow cytometers include detecting and enumerating
peripheral subsets of human T lymphocytes and performing
leukocyte differential cell counts, platelet autoantibody studies, cell
surface marker analysis, reticulocyte analysis, and bacterial
analysis. Flow cytometers also detect aneuploidy (any deviation from an exact multiple of the diploid number of
chromosomes, whether fewer or more) by performing intracellular measurements and analysis of DNA.
Clinicians can use the results of these studies, along with other clinical data, to diagnose and/or prognose
leukemia, lymphoma, immunodeficiency disorders such as human immunodeficiency virus (HIV) infection,
autoimmune disease, and fetal abnormalities, as well as evaluate the success of transplantation procedures. Flow
cytometry is commonly used in cancer diagnosis and research because it is ideal for evaluating drug resistance,
detecting tumor cell DNA aneuploidy, immunophenotyping, and analyzing tumor cell proliferation. In addition,
flow cytometry technology can be adapted to provide a rapid, sensitive, and cost-effective means for detecting,
characterizing, and identifying bacteria.

Principles of operation
Flow cytometers consist of three basic systems: the fluidic
component that transports cells to the analysis chamber, the optical UMDNS Information
system with detection devices, and the data analysis and display
components (see Figure 1). This Product Comparison covers the following
device term and product code as listed in
Immunofluorescence is the basis for most flow cytometric assays. ECRI Institute’s Universal Medical Device
Nomenclature System™ (UMDNS™):
This technique relies on the use of dyes and fluorochromes to mark
Cytometers, Automated, Flow [16-867]
specific structures in or on the cell. When cells are treated with Cytometers, Automated, Flow, Sorting [16-503]
fluorochromes, these dyes bind to specific cellular components, such as

5200 Butler Pike, Plymouth Meeting, PA 19462-1298, USA  Tel +1 (610) 825-6000  Fax +1 (610) 834-1275  Web www.ecri.org  E-mail hpcs@ecri.org
Cytometers, Automated, Flow

Figure 1. Operating systems of a representative flow cytometer

DNA, RNA, cellular enzymes, membrane surface markers, or other antigens. When exposed to light of a
particular wavelength, these fluorochromes will fluoresce, emitting light of a longer wavelength than that of the
incident light they absorb.
Two or more fluorochromes can be used simultaneously to label two cellular constituents (e.g., nuclear DNA
and cytoplasmic enzymes). In addition, multiple fluorochromes can be used to label the same cellular material.
Cellular components can also be labeled with a metachromatic stain such as acridine orange, whose molecular
location on different cellular components results in light emission at several different wavelengths.
Cells to be analyzed are mixed with specific reagents and then automatically suspended in a liquid stream that
is transported in a fluid medium under pressure to the analysis chamber. Before entering the chamber, the sample
stream is hydrodynamically focused by a sheathing solution that surrounds the stream, establishing a laminar
flow. This restricts the cells to a precise single-cell path that traverses the optical sensing area. Within this area,
the detection system analyzes each cell at a counting rate of up to 10,000 cells per second.
As the cells pass through the analysis chamber, they are illuminated by a beam of high-intensity
monochromatic light from a laser. The wavelength emitted by the laser needs to fall within the range of the
excitation energy of the chosen dyes/fluorochromes in order for fluorescence to take place. Many flow cytometers
today are equipped with two or more lasers, which can be used to excite a range of dyes and fluorochromes.
Argon lasers produce a strong emission at a wavelength of 488 nm. Argon lasers, which are air cooled, are
generally less expensive to operate and are the most common type used. Recently, with the development of solid-
state lasers, additional lasers at additional wavelengths can be used. Helium-neon (633 nm), helium-cadmium
(325 nm), and red-diode (635 nm) are a few other types of lasers that are now commonly used in flow cytometers.
These lasers are usually compact and made to work with typical voltage requirements; they can often be added to
existing flow cytometers. The use of additional lasers with different wavelengths has increased the number of
fluorescent probes that can be used and thus, the variety of tests that can be performed.
In addition to matching the excitation energy, it is also important that the wavelength emitted by the laser be
distinguishable from the wavelength of the fluorescence energy. (See table for a list of commonly used dyes and
fluorochromes.) As the light passes through the cells, the instrument measures the fluorescence and light scatter
that is emitted. Light scatter is a measure of the laser light that is reflected and refracted through the cell, as
opposed to the light that is emitted by the fluorescing dyes. Light scatter intensity measured in the forward

2 ©2009 ECRI Institute. All Rights Reserved

 Cytometers, Automated, Flow

direction is generally proportional to the cell’s cross-sectional area, while the light scatter at a right angle to the
incident light generally relates to the internal structures of the cell (e.g., nuclear to cytoplasmic ratios, granularity
of the cytoplasm). Light scatter can distinguish live cells from dead ones in a homogeneous population of
lymphocytes.
The collection of fluorescent light emitted from the sample
stream usually occurs at a 90° angle from the beam of incident
light. Fluorescence detection is based on the specificity of a
fluorescent dye to some unique part of the cell. This interaction
aids in the analysis and separation of lymphocyte
subpopulations, the measurement of subsets of T-lymphocyte
populations, the evaluation of cell kinetics in normal and
neoplastic cell populations, the measurement of cell surface
antigens (tumor markers, receptors), and the separation of cells
by membrane characteristics.
Flow cytometers with multiple lasers may be set up to run a
sequential analysis, resulting in fluorescence signals that are
spatially separated. In these cases, the separate signals can be
sent in different directions for analysis by half-mirrors so that
no signal mixing takes place. However, in some instruments, all the signals are mixed as the light leaves the cell.
This setup relies on dichroic mirrors and signal filters to separate and refine the signals for analysis. Dichroic
mirrors are designed to reflect light above a specified wavelength while transmitting light at lower wavelengths.
Further filtration can also take place as the light enters the photomultiplier tubes. These detectors turn the light
emitted by the cells into electronic pulses, which are then amplified for digitization and subsequent computer
analysis.
Some flow cytometers have cell-sorting
capabilities and can separate and analyze the
specific cell types within the sample. As the
sample stream exits the detection chamber, a
piezoelectric crystal (a crystal in which an applied
voltage generates a proportional mechanical
force) vibrates, breaking the sample stream into
droplets that pass between two polarized
deflection plates. Based on predetermined
selection criteria, individual cells bearing a net-
positive or a net-negative charge are deflected
into their respective collection vessels, while the
remaining fluid and cells are discarded as waste.
One type of cell-sorting flow cytometer features a mechanical sorting mechanism in which a catcher tube
positioned inside the flow cell moves in and out of the sample stream to capture cells of interest. This type of
sorting technology does not depend on droplet formation and occurs in an enclosed environment, thus reducing
the formation of aerosols and the risk of contamination from biohazardous samples.
Once the cell data has been captured by the detector, software records, analyzes, and displays the data on a
computer screen. Most systems incorporate printers and archiving storage, and some systems interface to the
laboratory’s information system (LIS).
Many systems today have autoloading capabilities. Autoloaders accept trays or carousels of sample tubes and
use robotic arms to automatically load the samples into the analyzer. Systems with autoloaders typically include
bar-code readers to keep track of sample information. In addition, automated sample preparation systems

©2009 ECRI Institute. All Rights Reserved 3

Cytometers, Automated, Flow

provide strict quality control and standardization of preparations and reduce preparation times of 1 to 2 hours to
10 minutes or less.

Reported problems
Analysis using flow cytometry requires that cells in suspension be monodispersed (i.e., each cell is surrounded
by a fluid matrix without aggregating to neighboring cells). Blood, bone marrow, and cell cultures readily form
single-cell suspensions; however, nonlymphoid solid tissue requires processing with either enzymes or chelating
agents to disrupt intercellular bonding. Unfortunately, this processing also produces cellular debris (cell stroma,
free nucleoplasm, and cytoplasm) that presents artifacts during analysis. Electronic discrimination of data can
prevent such debris from affecting the final results.
For a flow cytometer to work effectively, the point of intersection between the laser beam and the stream of
cells must be precisely maintained in relation to the detection pathway. This specific configuration requires
frequent performance checks on a daily basis to ensure proper instrument function.

Purchase considerations
ECRI Institute recommendations
Included in the accompanying comparison chart are ECRI Institute’s recommendations for minimum
performance requirements for flow cytometers. Because the applications of flow cytometry vary, buyers should
evaluate their needs and choose a device based on their specific requirements.
Flow cytometers should come equipped with software that performs immunophenotyping. Other software
applications (e.g., transplantation, DNA S-phase, microbiology) are optional and should be considered according
to the needs of a specific site.
Wavelength requirements for flow cytometers vary based on the fluorochromes being used in the analysis. A
wavelength of 488 nm is required when working with two or three colors; this laser wavelength is standard with
most flow cytometers. Additional laser wavelengths are preferable if the laboratory has additional detection
needs. ECRI Institute considers systems that detect two to three colors a requirement. Devices that can detect four
colors are preferred, and the analysis of six or more colors should be considered optional.
Cell sorting should be considered an optional feature. Buyers will need to evaluate whether sorting is a
required ability for their facility.

Other considerations
Because flow cytometer operation requires special training, manufacturers typically provide free instruction.
However, additional training and frequent operation of the instrument are needed for operators to become
proficient. For this reason, some facilities dedicate certain technicians for flow cytometry measurement. Hospitals
must also communicate with physicians to determine how these results will be used and what tests should be
performed.
Because flow cytometers are open-platform instruments, reagents can be purchased from many suppliers, and
it is generally not necessary to standardize the manufacturer of the flow cytometer and reagents. ECRI Institute’s
PriceGuide provides benchmark pricing data on reagents, sample vials, and other consumables; facilities should
contact PriceGuide for assistance securing the best price on consumables.
Laptop or desktop computers are typically included in the quoted price; printers are frequently an add-on
feature. Although most flow cytometers come standard with one or two basic software applications, there are
numerous applications available. Facilities may choose to purchase additional software packages from other
suppliers; some suppliers may offer custom-designed packages. Other basic software programs for research use

4 ©2009 ECRI Institute. All Rights Reserved

 Cytometers, Automated, Flow

can be downloaded at no cost from university Web sites. Software should be chosen based on the tests currently
being performed and tests expected to be performed in the near future, and whether the instrument will need to
be upgraded to accommodate new software installs.
Autosamplers, which help streamline laboratory workflow by increasing walkaway time, are often available as
upgradable options if the laboratory does not choose to purchase a system with an autosampler initially.

Environmental considerations
Developments in so-called “smart” solid-state light source technology have enabled the use of solid-state lasers
in flow cytometry. Solid-state light sources are extremely energy efficient and may improve the clarity of images.
Some systems may offer other energy-saving features, such as standby mode.
Facilities should consider flow cytometers with biohazard containment a required feature. In addition,
components made from recyclable materials and systems manufactured in green plants are desirable.
End-of-life costs need to be considered as well. Facilities should look for manufacturers who offer take-back
programs on system components such as monitors. If a supplier does not offer such an arrangement, the
laboratory must absorb the costs of disposing the system according to local environmental protection laws when
it is replaced.

Cost containment
Facilities should negotiate the service contract price from the manufacturer of the flow cytometer at the time of
purchase. Service contracts typically cost between 11% and 14% of the purchase price; in general, service contracts
must be purchased from the manufacturer because the instruments are highly sophisticated.
The cost of the reagents is a significant expenditure over the useful life of the instrument. Users need to be
aware that reimbursement policies regarding flow cytometry tests vary by state and by medical insurance carrier
and that these policies are in transition.
Because flow cytometers entail ongoing maintenance and operational costs, the initial acquisition cost does not
accurately reflect the total cost of ownership. Therefore, a purchase decision should be based on issues such as
life-cycle cost (LCC), local service support, discount rates and non-price-related benefits offered by the supplier,
and standardization with existing equipment in the laboratory (i.e., purchasing all flow cytometers from one
supplier).
Because it examines the cash-flow impact of initial acquisition costs and operating costs over a period of time,
LCC analysis is most useful for comparing alternatives with different cash flows and for revealing the total costs
of equipment ownership. One LCC technique—present value (PV) analysis—is especially useful because it
accounts for inflation and for the time value of money (i.e., money received today is worth more than money
received at a later date). Conducting a PV/LCC analysis often demonstrates that the cost of ownership includes
more than just the initial acquisition cost and that a small increase in initial acquisition cost may produce
significant savings in long-term operating costs. The PV is calculated using the annual cash outflow, the dollar
discount factor (the cost of capital), and the lifetime of the equipment (in years) in a mathematical equation.
The following represents a sample PV/LCC analysis for a flow cytometer.

Present Value/Life-Cycle Cost Analysis

Assumptions
 Operating costs are considered for years 1 through 5
 Dollar discount factor is 3.5%
 Inflation rate is 3% for support costs and 3% for disposables

©2009 ECRI Institute. All Rights Reserved 5

Cytometers, Automated, Flow

Capital Costs
 System = $140,000
Total Capital Costs = $140,000

Operating Costs
 Service contract, years 2 through 5 = $14,000/year
 Reagents = $105,000/year
Total Operating Costs = $145,000 for year 1; $159,000/year for years 2 through 5

PV = ($608,007)

Other costs not included in the above analysis that should be considered for budgetary planning include those
associated with the following:
 Software upgrades not included under warranty or in service contract
 Utilities
 Salary for 1 full time employee and related staffing expenses
 Contributions to overhead
As illustrated by the above sample PV/LCC analysis, the initial acquisition cost is only a fraction of the total
cost of operation over 5 years. Therefore, rather than making a purchase decision based solely on the acquisition
cost of a flow cytometer system, buyers should consider operating costs over the lifetime of the equipment.
For further information on PV/LCC analysis, customized analyses, and purchase decision support, readers
should contact ECRI Institute’s SELECTplus™ Group.

Stage of development
One direction flow cytometers may take in the near future is toward more affordable, durable light sources.
Researchers are investigating the replacement of laser light sources with high-power LEDs.
Data analysis software is continually being updated and expanded to improve the classification, presentation,
and analysis of data. Newer systems can accommodate more types of sample vials, and some can detect 15 or
more colors.
One flow cytometer currently available offers imaging capabilities. The technology, which combines flow
cytometry with microscopy, performs cell quantifying and labeling and also allows technicians to view images of
each individual cell.
Researchers are beginning to explore the possibility of using flow cytometers as virus detectors. Viruses,
however, are much smaller than bacteria or cancer cells, with diameters of 100 to 250 nm. Their minute size
makes it difficult to distinguish their light scatter signals from general background noise. As researchers continue
to work on maximizing the sensitivity of flow cytometers, the role of these devices as virus detectors will
continue to develop. In addition, analyses of tumor-cell DNA, as well as many clinical microbiological
applications, continue to be investigated as the uses for flow cytometry grow steadily in scope and importance.
In general, manufacturers strive to increase the sensitivity and complexity of flow cytometers while
maintaining a user-friendly platform, as the number of applications for flow cytometry continues to expand.

Bibliography
Agrawal S. Immunophenotyping using flow cytometry [online]. Express Healthc Manage 2004 Jul [cited 2009 Nov
12]. Available from Internet: http://www.expresshealthcaremgmt.com.

6 ©2009 ECRI Institute. All Rights Reserved

 Cytometers, Automated, Flow

Alvarez-Barrientos A, Arroyo J, Canton R, et al. Applications of flow cytometry in clinical microbiology. Clin
Microbiol Rev 2000 Apr;13(2):167-95.
Dessing M. Cell sorting and flow cytometry [online]. Euro Lab Sci 2004 Aug [cited 2009 Nov 12]. Available from
Internet: http://www.scientistlive.com.
Dijkstra-Tiekstra MJ, Schrijver JG, van der Meer PF, et al. Crossover study of three methods for low-level white
blood cell counting on two types of flow cytometer. Cytometry B Clin Cytom 2003 Jul;54(1):39-45.
Maino VC, Maecker HT. Cytokine flow cytometry: a multiparametric approach for assessing cellular immune
responses to viral antigens. Clin Immunol 2004 Mar;110(3):222-31.
McCoy, JP Jr. Basic principles of flow cytometry. Hematol Oncol Clin N Am 2002 Apr;16(2):229-43.
Minerd J. Buyer’s guide to flow cytometers. The Scientist 2005 Oct;19(20):28.
Reeve L, Rew DA. New technology in the analytical cell sciences: the laser scanning cytometer. Eur J Surg Oncol
1997 Oct;23(5):445-55.
Robinson K. Flow cytometer detects viruses smaller than 100 nm. Biophotonics Int 2004 Apr;11(4):19-20.
Shapiro HM. Practical flow cytometry. 4th ed. Hoboken (NJ): Wiley-Liss; 2003.
Smith C. Flow cytometry gets a makeover. Biocompare 2009 Sep [cited 2009 Nov 5]. Available from Internet:
http://www.biocompare.com.
Telford WG, Hubert CR. Solid-state lasers expand flow cytometry probe library. Biophot Int 2006 Feb;13(2):50-3.

Supplier information
ACCURI CYTOMETERS
Accuri Cytometers Inc [452700]
173 Parkland Plaza
Ann Arbor, MI 48103
Phone: (734) 994-8000 Fax: (734) 994-8002
Internet: http://www.accuricytometers.com
E-mail: info@accuricytometers.com

AMNIS
Amnis Corp [454607]
2505 Third Ave Suite 210
Seattle, WA 98121
Phone: (206) 374-7000, (800) 730-7147 Fax: (206) 576-6895
Internet: http://www.amnis.com
E-mail: sales@amnis.com

BD BIOSCIENCES
BD Biosciences (CA) Div BD [378353]
2350 Qume Dr
San Jose, CA 95131-1807
Phone: (408) 432-9475, (877) 232-8995 Fax: (408) 954-2347
Internet: http://www.bdbiosciences.com
E-mail: bdbcustomerservice@bd.com

BD India Div BD [378367]

6/FL Signature Tower-B South City 1 NH 8

©2009 ECRI Institute. All Rights Reserved 7

Cytometers, Automated, Flow

Gurgaon 122001
India
Phone: 91 (124) 238356671 Fax: 91 (124) 23832242526
Internet: http://www.bd.com
E-mail: bd_india@bd.com

BD Biosciences Div BD European Headquarters [378370]

Erembodegem - Dorp 86
Erembodegem/Aalst B-9320
Belgium
Phone: 32 (53) 720600 Fax: 32 (53) 720220
Internet: http://www.bdeurope.com
E-mail: biosciences_bnl@europe.bd.com

BECKMAN COULTER
Beckman Coulter Inc [340083]
4300 N Harbor Blvd PO Box 3100
Fullerton, CA 92834-3100
Phone: (714) 871-4848, (800) 232-3828 Fax: (714) 773-8283, (800) 643-4366
Internet: http://www.beckmancoulter.com
E-mail: askpcd@beckman.com

Beckman Coulter GmbH [175266]

Europark Fichtenhain B13
Krefeld D-47807
Germany
Phone: 51 (2151) 3335 Fax: 49 (2151) 333633
Internet: http://www.beckman.com
E-mail: bioresearch.de@beckman.com

Beckman Coulter KK [175265]

TOC Ariake West Tower 2-5-7 Ariake Koto-ku
Tokyo 135-0063
Japan
Phone: 81 (3) 67454704 Fax: 81 (3) 55302460
Internet: http://www.beckmancoulter.com

PARTEC
Partec GmbH [452983]
Otto-Hahn-Strasse 32
Muenster D-48161
Germany
Phone: 49 (2534) 80080 Fax: 49 (2534) 800890
Internet: http://www.partec.com
E-mail: info@partec.com

Partec North America Inc [452984]

603 Heron Dr Unit 9
Swedesboro, NJ 08085
Phone: (856) 467-0018, (888) 808-0067 Fax: (856) 467-0188
Internet: http://www.partec.com
E-mail: partecna@partec.com

8 ©2009 ECRI Institute. All Rights Reserved

 Cytometers, Automated, Flow

STRATEDIGM
Stratedigm Inc [454606]
1300 White Oaks Rd Suite 105
Campbell, CA 95008
Phone: (408) 512-3901 Fax: (408) 351-7700
Internet: http://www.stratedigm.com
E-mail: info@stratedigm.com

Note: The data in the charts derive from suppliers’ specifications and have not been verified through
independent testing by ECRI Institute or any other agency. Because test methods vary, different products’
specifications are not always comparable. Moreover, products and specifications are subject to frequent changes.
ECRI Institute is not responsible for the quality or validity of the information presented or for any adverse
consequences of acting on such information.
When reading the charts, keep in mind that, unless otherwise noted, the list price does not reflect supplier
discounts. And although we try to indicate which features and characteristics are standard and which are not,
some may be optional, at additional cost.
For those models whose prices were supplied to us in currencies other than U.S. dollars, we have also listed the
conversion to U.S. dollars to facilitate comparison among models. However, keep in mind that exchange rates change
often.

Need to know more?

For further information about the contents of this Product Comparison, contact the HPCS Hotline at +1 (610)
825-6000, ext. 5265; +1 (610) 834-1275 (fax); or hpcs@ecri.org (e-mail).

Last updated December 2009

©2009 ECRI Institute. All Rights Reserved 9

Cytometers, Automated, Flow,

Policy Statement
The Healthcare Product Comparison System (HPCS) is published by ECRI Institute, a nonprofit organization.
HPCS provides comprehensive information to help healthcare professionals select and purchase diagnostic and
therapeutic capital equipment more effectively in support of improved patient care.
The information in Product Comparisons comes from a number of sources: medical and biomedical
engineering literature, correspondence and discussion with manufacturers and distributors, specifications from
product literature, and ECRI Institute’s Problem Reporting System. While these data are reviewed by qualified
health professionals, they have not been tested by ECRI Institute’s clinical and engineering personnel and are
largely unconfirmed. The Healthcare Product Comparison System and ECRI Institute are not responsible for the
quality or validity of information derived from outside sources or for any adverse consequences of acting on such
information.
The appearance or listing of any item, or the use of a photograph thereof, in the Healthcare Product Comparison
System does not constitute the endorsement or approval of the product’s quality, performance, or value, or of
claims made for it by the manufacturer. The information and photographs published in Product Comparisons
appear at no charge to manufacturers.
Many of the words or model descriptions appearing in the Healthcare Product Comparison System are
proprietary names (e.g., trademarks), even though no reference to this fact may be made. The appearance of any
name without designation as proprietary should not be regarded as a representation that is not the subject of
proprietary rights.
ECRI Institute respects and is impartial to all ethical medical device companies and practices. The Healthcare
Product Comparison System accepts no advertising and has no obligations to any commercial interests. ECRI
Institute and its employees accept no royalties, gifts, finder’s fees, or commissions from the medical device
industry, nor do they own stock in medical device companies. Employees engage in no private consulting work
for the medical device industry.

About ECRI Institute

ECRI Institute, a nonprofit organization, dedicates itself to bringing the discipline of applied scientific research
in healthcare to uncover the best approaches to improving patient care. As pioneers in this science for over 40
years, ECRI Institute marries experience and independence with the objectivity of evidence-based research.
More than 5,000 healthcare organizations worldwide rely on ECRI Institute’s expertise in patient safety
improvement, risk and quality management, healthcare processes, devices, procedures, and drug technology.
ECRI Institute is one of only a handful of organizations designated as both a Collaborating Center of the World
Health Organization and an Evidence-based Practice Center by the U.S. Agency for Healthcare Research and
Quality. For more information, visit http://www.ecri.org.

10 ©2009 ECRI Institute. All Rights Reserved.

 Cytometers, Flow, Clinical Laboratory

Product Comparison Chart

©2009 ECRI Institute. All Rights Reserved 11

Cytometers, Automated, Flow,
Product Comparison Chart
MODEL ECRI INSTITUTE'S ACCURI CYTOMETERS
RECOMMENDED
SPECIFICATIONS1
Flow Cytometers C6 Flow Cytometer with
CFLow Plus Software
WHERE MARKETED Austria, Belguim, Canada,
Denmark, Finland, France,
Germany, Iceland, Ireland,
Liechtenstein, Luxembourg,
Netherlands, Norway,
Sweden, Switzerland, UK,
USA
FDA CLEARANCE No
CE MARK (MDD) Submitted
CLINICAL APPLICATIONS Immunophenotyping NA
required; transplantations,
DNA S-phase, and
microbiology applications
optional
RESEARCH To user requirements Immunophenotyping, DNA
APPLICATIONS analysis, cell cycle analysis,
proliferation, RNAi
knockdown, transfection
efficiency, cell counting,
viability, microbiological
applications, immunological
applications
SAMPLE VIALS, type 12 x 75 mm tubes,
microtubes without caps
Number With C-Sampler 48 and 96-
well plates (u, v and deep-
well), 24-tube rack for 12 x
75 mm tubes
AUTOLOADER Optional C-Sampler
Bar-code reader Required for instruments Not specified
with autosampler
12 ©2009 ECRI Institute. All Rights Reserved.
AMNIS
ImageStreamX Imaging
Flow Cytometer
Worldwide
No
Submitted
NA
Cell signaling, molecular
translocation, morphology
analysis, molecular co-
localization, cell-cell
interaction, cell cycle and
mitosis, DNA damage and
repair, cell death and
autophagy, spot counting,
FISH in suspension
Microcentrifuge tubes, 96-
well microplates
Single microcentrifuge tube
96-well microplate
autosampler
No
BD BIOSCIENCES
FACSCalibur
Worldwide
Yes
Yes
IVD for identification and
enumeration of lymphocyte
subsets (BD Multiset),
residual white blood cell
enumeration (BD
Leucocount), CD34
enumeration, BD CellQuest
Pro, BD HLA-B27, BD
Trucount bead technology
for absolute counts, BD
FACSComp instrument
setup
Dynamic gating (BD
Attractors), standardized
fluorescence quantitation
analysis (QuantiCALC),
Paint-A-Gate Pro multicolor
analysis software,
cytometric bead array (CBA)
analysis, optional ModFIT
LT DNA software, optional
sorting module
12 x 75 mm tubes; 96- and
384-well microtiter plates
when using BD high-
throughput sampler option
Not specified
Optional BD FACSLoader
for (40) tubes, BD high-
throughput sampler for
plates
Optional
This is the first of three
pages covering the above
model(s). These
specifications continue onto
the next two pages.
 Cytometers, Flow, Clinical Laboratory

Product Comparison Chart

MODEL ECRI INSTITUTE'S ACCURI CYTOMETERS AMNIS BD BIOSCIENCES

RECOMMENDED
SPECIFICATIONS1
Flow Cytometers C6 Flow Cytometer with ImageStreamX Imaging FACSCalibur
CFLow Plus Software Flow Cytometer
LIGHT SOURCE Diode lasers Air-cooled laser, solid-state Air-cooled 488 nm argon
laser laser; optional 635 nm diode
laser
Wavelength, nm 488 required 488, 635 488 standard; optional 405, 488, optional 635
560, 592, and 658
Fluorescence 2-3 colors required, 4 colors Dual laser, 4 colors; FL1 Up to 12 image detection Single laser, 3 colors; dual
preferred, ≥6 colors optional 530 ± 15 nm (FITC/GFP), channels laser, optional 4 colors
FL2 585 ± 20 nm (PE/PI),
FL3 >670 nm (PerCP-
Cy5.5, PE-Cy5, PE-Cy7),
FL4 675 ± 12.5 nm (APC)
Parameters measured To user requirements 4 fluorescent height/area, >1,000 parameters derived Forward scatter, 90°
forward scatter height/area, from side scatter (darkfield), scatter, 3 colors from 488
side scatter height/area, brightfield and fluorescence nm laser, optional 1
time, volume images additional color from 635 nm
diode laser; height, width,
area, time
LIGHT BEAM SIZE, µm 15 x 75 Not specified 22 x 66 (laser 1), 15 x 61
(laser 2)
FLOW CELL SIZE, µm 200 (diameter) Not specified 180 x 430
FLOW RATE 10 to 100 µL/min 65 mm/sec 12, 35, and 60 µL/min
DETECTOR
Range, nm 500-900 PMT 430-800 300-1,100
Sensitivity 750 MESF FITC <100 MESF <200 MESF FITC/particle
Minimum cell size, µm 1 0.5 µm Discriminates platelets from
noise
Maximum analysis rate, 10,000 Up to 2,000 Not specified
cells/sec
Cell sorting, cells/sec Sorting is optional NA NA 300
DATA MANAGEMENT
Display 257-channel full color 1920 x 1200 256- or 1,024-channel full-
resolution color resolution; multiple
parameters on histograms
and dot, contour, isometric,
and color-density plots; real-
time statistics, 19" flat-
screen monitor
Microprocessor 2.0 GHz Intel Pentium 4 Dual quad-core Intel E55XX MacPro 2.8 GHz, Quad-
Core Intel Xeon processor
Storage 2 GB RAM, 160 GB HD, CD 250 GB 2 GB RAM, 320 GB HD, 16x
drive DVD RW
Output 17" flat-panel monitor 1 GB Ethernet Standard FCS 2.0 data files;
high-speed PostScript
Ethernet color printer
Printer External Not specified Optional Ricoh printer
LIS interface Not specified Not specified Optional BD FACSLink LIS
interface powered by
Instrument Manager from
Data Innovations
CLEANING Auto-cleaning parameters Automatic cycle Not specified
built-in

This is the second of three

pages covering the above
model(s). These
specifications continue onto
the next page.

©2009 ECRI Institute. All Rights Reserved 13

Cytometers, Automated, Flow,

Product Comparison Chart

MODEL ECRI INSTITUTE'S ACCURI CYTOMETERS AMNIS BD BIOSCIENCES

RECOMMENDED
SPECIFICATIONS1
Flow Cytometers C6 Flow Cytometer with ImageStreamX Imaging FACSCalibur
CFLow Plus Software Flow Cytometer
H x W x D, cm (in) 27.9 x 36.3 x 41.9 (11 x 91 x 66 x 61 (36 x 26 x 24) 67.3 x 91.4 x 61.5 (26.5 x
14.3 x 16.5) 36 x 24.2) cytometer, 54 x
48 x 41 (21.2 x 18.9 x 16.2)
computer
WEIGHT, kg (lb) 14 (30) 159 (350) 109.1 (240) sensor, 20 (44)
computer
POWER, VAC 100-240 90-240, 50-60 Hz 120/240
PURCHASE
INFORMATION
List price $40,000 $210,000 $119,850-130,250
Warranty Required 1 year 1 year 1 year
Delivery time, ARO 4 weeks 6-8 weeks Not specified
Training Included, on-site Introductory and advanced Included
training on-site or off-site
GREEN FEATURES Uses de-ionized water as All solid-state light sources None specified
sheath
OTHER SPECIFICATIONS User-adjustable core flow None specified. Benchtop analyzer and
speed; automatic sorter; simultaneous 4-color
decontamination and analysis; no external air or
cleaning; accepts water required; aerosol-free
microcentrifuge tubes; USB sorting; selectable sample
interface; <5 min warm-up flow rates; optional 40-tube
time, fully digital, 6 decades carousel loader; FACSLink
of dynamic range, fixed LIS interface provides bi-
alignment; core diameter is directional LIS connectivity
user-selectable from 5-40 for BD FACS SPA software,
µm; 90% and 99% BD Worklist Manager
attenuation filters available software, BD Multiset
for FL1, FL2, FL3 and FL4; software, and BD HLA-B27
optical filter 510 ± 7.5 nm, software.
565 ± 10 nm, 610 nm ± 10
nm, 780 ± 30 nm available.
UMDNS CODE(S) 16867, 16503 16867 16867 16867, 16503
LAST UPDATED December 2009 December 2009 December 2009
Supplier Footnotes 1These recommendations
are the opinions of ECRI
Institute's technology
experts. ECRI Institute
assumes no liability for
decisions made based on
this data.
Model Footnotes
Data Footnotes

14 ©2009 ECRI Institute. All Rights Reserved.

 Cytometers, Flow, Clinical Laboratory

Product Comparison Chart

MODEL BD BIOSCIENCES BECKMAN COULTER BECKMAN COULTER BECKMAN COULTER

FACSCanto II Cytomics FC 500 Flow Cytomics FC 500 Multi Plate EPICS XL : EPICS XL-MCL
Cytometer Loader Cytometer with FCW
WHERE MARKETED Worldwide Worldwide Worldwide Worldwide
FDA CLEARANCE Yes Yes Yes Yes
CE MARK (MDD) Yes Yes Yes Yes
CLINICAL APPLICATIONS IVD for identification and IVD-cleared HIV 2-, 3-, 4-, 1-, 2-, 3-, 4-, and 5-color IVD-cleared HIV 2-, 3-, and
enumeration of lymphocyte and 5-color immunophenotyping, 4-color immunophenotyping,
subsets (BD FACSCanto immunophenotyping, including direct absolute including direct absolute
software); CD34 including direct absolute counts; CD 34 analysis counts; reticulocyte
enumeration, BD HLA-B27, counts enumeration; CD 34
BD Trucount bead analysis
technology for absolute
counts, BD FACS 7-color
setup, beads instrument
setup, BD FACSDiva
software for 6- or 8-color
immunophenotyping
RESEARCH 6-color or 8-color analysis of Leukemia and transplant Leukemia and transplant Leukemia and transplant
APPLICATIONS lysed whole blood and cell immunophenotyping; immunophenotyping; immunophenotyping;
suspensions, DNA analysis DNA/cell-cycle analysis; DNA/cell-cycle analysis; DNA/cell-cycle analysis;
activation; apoptosis; bead- activation; apoptosis; bead- activation; apoptosis
based assays based assays
SAMPLE VIALS, type Optional 40-tube carousel 12 x 75 mm tubes Plates and tubes 12 x 75 mm tubes
loader
Number Not specified 32 Not specified 32 w MCL
AUTOLOADER Optional BD SPA III Standard Standard Optional
Bar-code reader Optional Tube, carousel and position Not specified Tube, carousel and position
(with MCL)

This is the first of three

pages covering the above
model(s). These
specifications continue onto
the next two pages.

©2009 ECRI Institute. All Rights Reserved 15

Cytometers, Automated, Flow,
MODEL BD BIOSCIENCES
FACSCanto II
LIGHT SOURCE Solid-state 488 nm laser;
633 nm HeNe laser
Wavelength, nm 488, 633
Fluorescence Dual laser, 6 or 8 colors
Parameters measured Forward scatter, 90°
scatter, 4 colors from 488
nm laser, 2 colors from 633
nm laser; area, height,
width, time, ratio on any
parameter
LIGHT BEAM SIZE, µm 9 x 65 spatially separated
elliptical spots
FLOW CELL SIZE, µm 180 x 430
FLOW RATE Not specified
DETECTOR
Range, nm 300-1,100
Sensitivity <100 MESF FITC, <50
MESF PE
Minimum cell size, µm 1 <0.5
Maximum analysis rate, Up to 10,000
cells/sec
Cell sorting, cells/sec NA
DATA MANAGEMENT
Display 18-bit data acquisition;
262,144 linear channel; 5-
decade logarithmic display
resolution; multiple
parameters on histograms
and dot, contour, isometric,
and color-density plots;
offline compensation; real-
time statistics; 19" or 23"
flat-screen monitor
Microprocessor 3.0 GHz Intel Duo Core
processor
Storage 2 GB RAM, dual hard drives
(250, 80 GB), 16x DVD-RW
Output FCS 3.0, exportable in FCS
2.0, high-speed Ethernet
color printer
Printer Optional Ricoh printer
LIS interface Optional BD FACSLink LIS
interface powered by
Instrument Manager from
Data Innovations
CLEANING Not specified
16
Product Comparison Chart
BECKMAN COULTER
Cytomics FC 500 Flow
Cytometer
Mini air-cooled 15 mW
argon-ion laser and air-
cooled 23 mW diode laser,
collinear
488, 633
5-color; interchangeable
filters
21, 11 x 1,024 channel; 8
acquired simultaneously;
auxiliary, PRISM, time, and
ratio
10 x 80
150 x 450, quartz
Not specified
185-900
<600 molecules, FITC,
APC; <300 molecules PE
>3,300
NA
Color-dot density; contour,
isometric, tomogram,
overlay, legend, and
projection plots; PRISM
displays; color precedence
or color-blend gating;
automatic Boolean-logic
gate creation; 32 gates/file;
real-time statistics
Intel Pentium 4 processor
and 3 network transputers
for digital signal processing
80 GB HD, 3.5" floppy drive,
40x IDE CD-ROM and SCSI
2 support, network card,
256 MB RAM, internal
recordable CD, Zip drive,
Jaz drive, 1.7 GHz
processor
2 x 17" flat-panel monitor
with built-in speakers, swivel
option
Not specified
Not specified
Not specified
©2009 ECRI Institute. All Rights Reserved.
BECKMAN COULTER
Cytomics FC 500 Multi Plate
Loader
Mini air-cooled 15 mW
argon-ion laser and air-
cooled 23 mW diode laser,
collinear
488, 633
5-color; interchangeable
filters
21, 11 x 1,024 channel; 8
acquired simultaneously;
auxiliary, PRISM, time, and
ratio
10 x 80
150 x 450, quartz
Not specified
185-900
<600 molecules, FITC,
APC; <300 molecules, PE
<0.5
>3,300
NA
Color-dot density; contour,
isometric, tomogram,
overlay, legend, and
projection plots; PRISM
displays; color precedence
or color-blend gating;
automatic Boolean-logic
gate creation; 32 gates/file;
real-time statistics
Intel Pentium 4 processor
and 3 network transputers
for digital signal processing
80 GB HD, 3.5" floppy drive,
40x IDE CD-ROM and SCSI
2 support, network card,
256 MB RAM, internal
recordable CD, Zip drive,
Jaz drive, 1.7 GHz
processor
2 x 17" flat-panel monitor
with built-in speakers, swivel
option
Not specified
Not specified
Not specified
BECKMAN COULTER
EPICS XL : EPICS XL-MCL
Cytometer with FCW
Mini air-cooled 15 mW
argon-ion laser
488
4-color; interchangeable
filters
21, 11 x 1,024 channel; 8
acquired simultaneously;
auxiliary, PRISM, time, and
ratio
10 x 80
250 x 250, quartz
Not specified
300-800, optional far-red
PMT 300-950
<1,000 molecules, FITC, PE
<0.5
>3,300
NA
Color-dot density; contour,
isometric, tomogram,
overlay, legend, and
projection plots; PRISM
displays; color precedence
or color-blend gating;
automatic Boolean-logic
gate creation; 32 gates/file;
real-time statistics
Intel Pentium 3 processor
and 4 network transputers
for digital signal processing
30 GB HD, 3.5" floppy drive,
40x IDE CD-ROM and SCSI
2 support, network card,
128 MB RAM, optional
internal recordable CD, Zip
drive, Jaz drive
15" or 17" flat-panel monitor
with built-in speakers, swivel
option
Not specified
Not specified
Not specified
This is the second of three
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model(s). These
specifications continue onto
the next page.
 Cytometers, Flow, Clinical Laboratory

Product Comparison Chart

MODEL BD BIOSCIENCES BECKMAN COULTER BECKMAN COULTER BECKMAN COULTER

FACSCanto II Cytomics FC 500 Flow Cytomics FC 500 Multi Plate EPICS XL : EPICS XL-MCL
Cytometer Loader Cytometer with FCW
H x W x D, cm (in) 61 x 90.2 x 63.5 (24 x 35.5 61 x 111.8 x 105.4 (24 x 44 61 x 97.8 x 105.4 (24 x 38.5 50.8 x 61 x 57.2 (20 x 24 x
x 25) cytometer, 66 x 81.3 x x 41.5) cytometer, 15.8 x x 41.5) cytometer, 15.8 x 22.5) : 50.8 x 86.6 x 57.2
66 (26 x 32 x 26) fluidics 53.3 x 42.6 (6.2 x 21 x 16.8) 53.3 x 42.6 (6.2 x 21 x 16.8) (20 x 34.1 x 22.5)
cart workstation workstation
WEIGHT, kg (lb) 149 (330) cytometer, 45 93.9 (207) 93.9 (207) 63.5 (140) : 84.8 (187)
(100) cart
POWER, VAC 120/240 100/115/220/240 100/115/220/240 100/115/220/240
PURCHASE
INFORMATION
List price $233,220 : $189,000 $139,200-174,180 $176,340 $126,000
Warranty 1 year 1 year 1 year 1 year
Delivery time, ARO Not specified 6-12 weeks 6-12 weeks 6-12 weeks
Training Included Included Included Included
GREEN FEATURES None specified None specified None specified None specified
OTHER SPECIFICATIONS Sample-to-sample carryover Signal-intensity display; Signal-intensity display; Signal-intensity display;
<0.1%; selectable sample biohazard-safe triple-fluid biohazard-safe triple-fluid biohazard-safe triple-fluid
flow rates; software- (sheath/cleanse/waste) (sheath/cleanse/waste) (sheath/cleanse/waste)
controlled start-up, cleaning, system with automated system with automated system with automated
and shutdown cycles; functions and variable flow functions and variable flow functions and variable flow
fluidics cart with all rate; autosetup; CXP rate; auto setup; MXP rate; autosetup; SYSTEM II
housekeeping reagents; BD software with acquisition; software with acquisition; SW with acquisition, list
FACSLink provides bi- FCS 3.0; 20-bit list mode; FCS 3.0; 20-bit list mode; mode, multigraph, reports,
directional LIS connectivity overlay; FlowPage reports overlay; FlowPage reports and utilities; FastSet and
for BD FACSCanto clinical and administrator features; and administrator features; CellTracker; optional
software. QuickComp; QuickSet and QuickComp; QuickSet and tetraONE SYSTEM software
color gating; optional color gating; automated automates 4-color
tetraCXP software plate loading. acquisition and analysis;
automates 4-color reticONE SYSTEM software
acquisition and analysis; for reticulocyte enumeration;
automated multitube XL-MCL incorporates
carousel loader; directly automated multitube
compatible with PrepPlus2, carousel loader; compatible
and TQPrep sample with PrepPlus and TQPrep
preparation systems plus sample preparation systems
integrated bar-code scanner for ~100 tubes/hr; integrated
for 3-way sample ID bar-code scanner with 3-
(carousel, tube position, and way ID, true vortex mixing,
sample tube). optional EXPO32 ADC
Windows-based acquisition
and software for automated
color comp setup.
UMDNS CODE(S) 16867, 16503 16867 16867 16867
LAST UPDATED December 2009 December 2009 December 2009 December 2009
Supplier Footnotes
Model Footnotes
Data Footnotes

©2009 ECRI Institute. All Rights Reserved 17

Cytometers, Automated, Flow,

Product Comparison Chart

MODEL BECKMAN COULTER PARTEC PARTEC PARTEC

Navios CyFlow Counter CyFlow ML CyFlow SL
WHERE MARKETED Europe Worldwide Worldwide Worldwide
FDA CLEARANCE Submitted No No No
CE MARK (MDD) Yes Yes Yes Yes
CLINICAL APPLICATIONS Immunophenotyping, Dedicated for routine Immunology, stem cell Immunology, stem cell
including direct absolute HIV/AIDS patient counting, pathology, cell counting, pathology, cell
counts; US IVD clearance monitoring, determination of culture, apoptosis culture, apoptosis
pending CD4+ T-lymphocyte
absolute count and
percentage among all
lymphocytes
RESEARCH Leukemia and transplant None specified Immunology, stem cell Immunology, stem cell
APPLICATIONS immunophenotyping; counting, pathology, cell counting, pathology, cell
DNA/cell-cycle analysis; culture, apoptosis; also culture, apoptosis; also
activation; apoptosis; bead- microbiology (bacteria, microbiology (bacteria,
based assays yeast, virus and submicron yeast, virus and submicron
particle detection) particle detection)
SAMPLE VIALS, type 12 x 75 mm tubes 3 mL, 5 mL 3 mL, 5 mL 3 mL, 5 mL
Number 32 1 1 1
AUTOLOADER Standard Yes, 40 samples Yes, 96-well plate Yes, 96-well plate
autoloader autoloader
Bar-code reader Tube, carousel and position Yes Yes Yes

This is the first of three

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model(s). These
specifications continue onto
the next two pages.

18 ©2009 ECRI Institute. All Rights Reserved.

 Cytometers, Flow, Clinical Laboratory

Product Comparison Chart

MODEL BECKMAN COULTER PARTEC PARTEC PARTEC

Navios CyFlow Counter CyFlow ML CyFlow SL
LIGHT SOURCE All solid state 488 (22mW), Solid-state laser Solid-state laser, diode laser, Solid-state laser
638 (25mW), 405 (40mW) HBO lamp, UV LED
Wavelength, nm 488, 633, 405 30 mW @ 532 (green) 20-200 mW @ 488 nm, 25-40 20 mW @ 488 nm, others
mW @ 635 nm, 30-100 mW
@ 532 nm, 50 mW @ 405
nm, 16 mW @ 375 nm, 100
mW @ 561 nm, 100 W HBO
lamp, high power 365 nm UV
LED, others
Fluorescence 6, 8 or 10 color 2 colors Up to 13 colors Up to 3 colors
configuration;
interchangeable filters
Parameters measured 62, 11 x 1,024 channel; 16 3 optical parameters (SSC Up to 16 optical parameters: Up to 5 optical
acquired simultaneously; and 2 fluorescence 2 different forward scatter parameters: forward
peak, integral, width (tof), channels) signals (FSC1-2) and 1 side scatter (FSC), side scatter
time, ratio scatter (SSC) in combination (SSC) in combination with
with up to 13 fluorescence up to 3 flourescence
channels (FL1-13) channels (FL1-3)
LIGHT BEAM SIZE, µm Elliptical; 10 x 84 (488 nm), Not specified (AlignFree Elliptical 20 x 60 µm laser Elliptical 20 x 60 µm laser
9.6 x 72 (638 nm), 8.9 x 70 optical system) spot at 488 nm spot at 488 nm
(405 nm)
FLOW CELL SIZE, µm 150 x 460, quartz 250 x 350 synthetic quartz 250 x 350 synthetic quartz 250 x 350 synthetic quartz
flow cuvette for laminar flow cuvette for laminar flow cuvette for laminar
sample transport with sample transport with sheath sample transport with
sheath fluid fluid sheath fluid
FLOW RATE Not specified 0.1-20 µL/sec 0.1-10 µL/sec 0.1-10 µL/sec
DETECTOR
Range, nm 185-900 530-750 400-900 400-900
Sensitivity <600 molecules, FITC, <50 MESF (PE) <100 MESF (FITC), <50 <100 MESF (FITC), <50
APC; <300 molecules, PE MESF (PE) MESF (PE)
Minimum cell size, µm <0.404 NA 0.2 0.2
Maximum analysis rate, >25,000 15,000 15,000 (24 plots, fully 15,000 (8 plots, fully
cells/sec compensated) compensated)
Cell sorting, cells/sec NA NA NA NA
DATA MANAGEMENT
Display Color-dot density; contour, 256 channels resolution for 64-32,768 channels 64-32,768 channels
isometric, tomogram, 1P histograms, 256/256 resolution for 1P histograms; resolution for 1P
overlay, legend, and channels for 2P dot plots 32/32-1024/1024 channels histograms; 32/32-
projection plots; PRISM for 2P dot plots 1024/1024 channels for 2P
displays; color precedence dot plots
or color-blend gating;
automatic Boolean-logic
gate creation; 32 gates/file;
real-time statistics
Microprocessor Intel Pentium Core2 Duo Intel Celeron computer Pentium processor ≥3.0 GHz Pentium processor ≥3.0
2.13GHz processor GHz
Storage 160 GB HD, 4GB Ram, Not specified ≥1 GB RAM, ≥160 GB hard ≥1 GB RAM, ≥160 GB
Pentium Core2 Duo disk hard disk
2.13GHz, DVD 18x, CD40x,
8 USB ports, Windows Vista
Business
Output 22" flat-panel monitor (dual 8.4" TFT color touchscreen 19“ TFT LCD display; color 17“ TFT LCD display; color
monitor capable) (SVGA) CCD camera for video flow CCD camera for video flow
monitor monitor
Printer Not specified Built-in thermoprinter Ink jet laser printer, others Ink jet laser printer, others
optional optional
LIS interface Not specified Optional Optional Optional
CLEANING Not specified Automatic, built-in Automatic, built-in Automatic, built-in

These specifications
continue onto the next
page

©2009 ECRI Institute. All Rights Reserved 19

Cytometers, Automated, Flow,
MODEL BECKMAN COULTER
Navios
H x W x D, cm (in) 61 x 95 x 70 (24 x 38 x 28)
cytometer, 43.2 x 20.3 x
45.7 (17 x 8 x 19)
workstation
WEIGHT, kg (lb) 104 (230)
POWER, VAC 100/115/220/240
PURCHASE
INFORMATION
List price $170,000-230,000
Warranty 1 year
Delivery time, ARO 6-12 weeks
Training Included
GREEN FEATURES None specified
OTHER SPECIFICATIONS Signal-intensity display;
biohazard-safe triple-fluid
(sheath/cleanse/waste)
system with automated
functions and variable flow
rate; auto setup; Navios
software with acquisition;
FCS 3.0; 20-bit list mode;
overlay; FlowPage reports
and administrator features;
QuickComp; QuickSet and
color gating; , compatible
with TQPrep, Multi-QPrep,
PrepPlus2. Pro-Service
compatible, 3 software
controlled settings for FS
angle collection (narrow,
wide and enhanced).
UMDNS CODE(S) 16867
LAST UPDATED December 2009
Supplier Footnotes
Model Footnotes
Data Footnotes
20
Product Comparison Chart
PARTEC
CyFlow Counter
33 x 32.5 x 26.5 (12.9 x
12.7 x 10.4)
9.7 (21.3)
100/240
€18,800 (US$27,698)
1 year
3-6 weeks
Installation and on-site
training for €1,950
(US$2,811)
None specified
Detector has parallel signal
processing for each optical
channel with selectable
linear; 3-decade logarithmic
scale; pulse height; 16-bit
analog-to-digital converters;
trigger on any parameter;
true volumetric absolute
counting based on
mechanical volume
measurement; no need for
reference particles; capacity
of up to 250 CD4/CD4%
tests per day; optional
autoloader upgrade. Meets
requirements of European
IVD.
16867
December 2009
©2009 ECRI Institute. All Rights Reserved.
PARTEC
CyFlow ML
30 x 56 x 65 (11.8 x 22 x
25.5)
37 (81.5)
100/240
€50,000-170,000
(US$73,666-250,489)
1 year
6 weeks
Installation and 2 days on-
site training included
None specified
Detector has parallel signal
processing for each of the
optical channels with
selectable linear; 3- or 4-
decade logarithmic scale;
pulse height, area, and
width analysis for doublet
discrimination; 16-bit
analog-to-digital converters;
trigger on any parameter; up
to 3 laser spots spatially
separated with time delay
for data processing; true
volumetric absolute
counting based on
mechanical volume
measurement; no need for
reference particles; flexible
and modular system
configuration; optional
upgrade to RobbyWell
autoloader. Meets
requirements of European
IVD.
16867
December 2009
PARTEC
CyFlow SL
16 x 43 x 37 (6.2 x 16.9 x
14.5)
17 (37.4)
100/240 or 12 VDC car
battery/solar panels
€43,520 (US$64,125)
1 year
6 weeks
Installation and 2 days on-
site training included
None specified
Detector has parallel signal
processing for each optical
channel with selectable
linear; 3- or 4-decade
logarithmic scale; pulse
height, area, and width
analysis for doublet
discrimination; 16-bit
analog-to-digital converters;
trigger on any parameter;
true volumetric absolute
counting based on
mechanical volume
measurement; no need for
reference particles; flexible
and modular system
configuration; optional
RobbyWell autoloader
upgrade. Meets
requirements of European
IVD.
16867
December 2009
 Cytometers, Flow, Clinical Laboratory

Product Comparison Chart

MODEL PARTEC STRATEDIGM

CyFlow Space S1000 Series : SE500
Series
WHERE MARKETED Worldwide USA
FDA CLEARANCE No No
CE MARK (MDD) Yes Yes
CLINICAL APPLICATIONS Immunology, stem cell NA
counting, pathology, cell
culture, apoptosis, sorting
RESEARCH Immunology, stem cell Cell culture, DNA S-phase,
APPLICATIONS counting, pathology, cell immunophenotyping,
culture, apoptosis, sorting; microparticle analysis, stem
also microbiology (bacteria, cell analysis, cancer
yeast, virus and submicron research
particle detection)
SAMPLE VIALS, type 3 mL, 5 mL Tubes
Number 1 1
AUTOLOADER Yes, 96-well plate No
autoloader
Bar-code reader Yes Yes

This is the first of three

pages covering the above
model(s). These
specifications continue onto
the next two pages.

©2009 ECRI Institute. All Rights Reserved 21

Cytometers, Automated, Flow,

Product Comparison Chart

MODEL PARTEC STRATEDIGM

CyFlow Space S1000 Series : SE500
Series
LIGHT SOURCE Solid-state laser, diode laser Air-cooled laser, solid-state
laser, diode, spatially
separated
Wavelength, nm 20-200 mW @ 488 nm, 25- 372, 405, 488, 532, 561,
40 mW @ 635 nm, 30-100 640
mW @ 532 nm, 50 mW @
405 nm, 16 mW @ 375 nm,
100 mW @ 561 nm, high
power 365 nm UV LED,
others
Fluorescence Up to 7 colors Up to 4 laser, 10 colors
Parameters measured Up to 9 optical parameters: Forward scatter, side
forward scatter (FSC), side scatter, time, true width, log
scatter (SSC) in and linear, height and area
combination with up to 7
flourescence channels (FL1-
7)
LIGHT BEAM SIZE, µm Elliptical 20 x 60 µm laser 14 x 80
spot at 488 nm
FLOW CELL SIZE, µm 250 x 350 synthetic quartz Not specified
flow cuvette for laminar
sample transport with
sheath fluid
FLOW RATE 0.1-10 µL/sec High and low
DETECTOR
Range, nm 400-900 300-900
Sensitivity <100 MESF (FITC), <50 <100 MESF FITC, <50
MESF (PE) MESF PE
Minimum cell size, µm 0.2 <0.5
Maximum analysis rate, 15,000 (8 plots, fully 10,000
cells/sec compensated)
Cell sorting, cells/sec 10,000 input, 300 output NA
DATA MANAGEMENT
Display 64-32,768 channels Up to 4,096 channel full-
resolution for 1P color resolution
histograms; 32/32-
1024/1024 channels for 2P
dot plots
Microprocessor Pentium processor ≥3.0 2.8 GHz Intel Pentium 4
GHz
Storage ≥1 GB RAM, ≥160 GB hard 2 GB RAM, 160 GB HD,
disk CD-RW
Output 19“ TFT LCD display; color 22" flat panel monitor,
CCD camera for video flow optional printer
monitor
Printer Ink jet laser printer, others Optional
optional
LIS interface Optional Optional
CLEANING Automatic, built-in Automatic cycle

This is the second of three

pages covering the above
model(s). These
specifications continue onto
the next page.

22 ©2009 ECRI Institute. All Rights Reserved.

 Cytometers, Flow, Clinical Laboratory

Product Comparison Chart

MODEL PARTEC STRATEDIGM

CyFlow Space S1000 Series : SE500
Series
H x W x D, cm (in) 30 x 56 x 65 (11.8 x 22 x 61 x 55 x 53 (24 x 21.5 x
25.5) 21) cytometer; 10 x 35.6 x
43 (4 x 14 x 16.5)
workstation
WEIGHT, kg (lb) 37 (81.5) 35 (<70) complete unit
POWER, VAC 100/240 120/240
PURCHASE
INFORMATION
List price €50,000-115,000 $49,000-199,000
(US$73,666-169,448)
Warranty 1 year 1 year
Delivery time, ARO 6 weeks 6-9 weeks
Training Installation and 2 days on- On-site
site training included
GREEN FEATURES None specified Green manufacturing plant;
items shipped in reusable
shipping crates; take-back
program offered; low power
consumption and hibernate
mode
OTHER SPECIFICATIONS Detector has parallel signal Upgradeable benchtop
processing for each of the system; web-based remote
optical channels with troubleshooting and
selectable linear; 3- or 4- support; easily replaceable
decade logarithmic scale; components minimizes
pulse height, area, and downtime.
width analysis for doublet
discrimination; 16 bit
analog-to-digital converters;
trigger on any parameter; up
to 3 laser spots spatially
separated with time delay
for data processing; true
volumetric absolute
counting based on
mechanical volume
measurement; no need for
reference particles; flexible
and modular system
configuration; optional
upgrade to Particle and Cell
Sorter PPCS or RobbyWell
autoloader. Meets
requirements of European
IVD.
UMDNS CODE(S) 16867, 16503 16867
LAST UPDATED December 2009 December 2009
Supplier Footnotes
Model Footnotes
Data Footnotes

©2009 ECRI Institute. All Rights Reserved 23