ALICYCLOBACILLUS

collection Flowchart
SAMPLE TYPE

Ketchup Tomato Orange Juice Apple Juice Peach Pineapple Cranberry

Sauce/Paste/Juice Filtered/Pulp Filtered/Unfiltered Juice/Puree Juice/Puree Juice/Puree
& Concentrate & Concentrate Puree /Concentrate & Concentrate & Concentrate & Concentrate Membrane Filters

Note: If Brix value is >20, samples must be diluted until Brix value is <20.
Measure out 10 mL of sample and 90 mL ± 10 mL of BAT or ACB media

ENRICHMENT PROCEDURE

10
10
min

min

Room Incubate for 48 ± 2 hours

Temperature at 45ºC ± 1ºC
Water Bath 20ºC - 25ºC
80ºC ± 1ºC

SAMPLE LYSIS

10
min
10
min

Room
5 µL 995 µL Heat Temperature
Sample Lysis Buffer 95ºC ± 3ºC 20ºC - 25ºC

SET-UP SIMUL-QPCR ASSAY

Place tubes in PCR and run

20 µL

OR

Pro32 Mini16

REVIEW RESULTS COLLECTION

Results within 1 hour
Alicyclobacillus species Assay
Negative Positive A. acidoterrestris & acidiphilus Assay
A. herbarius & cycloheptanicus Assay

SMRT_ACB_FC_V1
p 570 450 7995 | appliedfooddiagnostics.com | 18 Industrial Drive, Bloomsburg, PA 17815