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SEA TURTLES
Marine Science Series
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The
BIOLOGY
of
SEA TURTLES
Edited by
Peter L. Lutz
John A. Musick
CRC PR E SS
Boca Raton London New York Washington, D.C.
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Peter L. Lutz
John A. Musîck
Acknowledgment
The idea for this book was bom out of many late nights of discussions with friends
and colleagues at annual meetings of the Sea Turtle Symposium. This convocation
has grown since 1981 from a small group of about 60 scientists and conservationists
to more than 700 workers drawn from 30 nations each year. We are extremely grateful
for those who contributed chapters, as well as those who freely gave of their precious
time as reviewers. This book is dedicated to the collegial spirit of scholarship and
camaraderie embodied in the Annual Sea Turtle Symposium.
The Editors
Peter L. Lutz, Ph.D., holds the McGinty Eminent Scholar Chair in Marine Biology
at Florida Atlantic University. Dr. Lutz received both his B.Sc. and Ph.D. from
Glasgow University, Scotland. After finishing his Ph.D. in 1970 he became a research
associate with Dr. Knut Schmidt-Nielsen at Duke University, with whom he worked
on avian respiration. In 1972 he became a Lecturer at the University of Bath,
England, and in 1974 an Associate Professor in Marine Biology at the Rosenstile
School of Marine and Atmospheric Science, University of Miami. In 1982 he was
promoted to Professor and in 1983 became Chairman of Marine Biology and Fish
eries, a post he held until he took up his present position in 1991.
As a comparative physiologist, he has worked on the physiology of a wide
variety of organisms, from liver flukes to duck-billed platypuses. His current interests
center on survival strategies of anoxia-tolerant brains and on the physiology of sea
turtles. Dr. Lutz is a member of the Society for Neuroscience, the American Phys
iological Society, and the Society for Experimental Biology. He is Series Editor for
the Marine Science Series published by CRC Press. He was a Governing Council
Member, Bahamas National Trust, and is a Fellow of the Explorers Club and member
of the Species Survival Commission of the lUCN. He has authored more than 150
research papers and 2 books.
John A. (Jack) Mustek, Ph.D., received his B.A. in Biology from Rutgers University
in 1962 and his M.A. and Ph.D. from Harvard University in 1964 and 1969, respec
tively. He has been on the faculty at the Virginia Institute of Marine Science, College
of William and Mary since 1967 and has successfully mentored 28 M.A. and 30
Ph.D. students. His research has covered many aspects of vertebrate ecology includ
ing community structure in deep sea fishes, shark population dynamics, and sea
turtle ecology. In 1985, he was elected a Fellow by the American Association for
the Advancement of Science. He has served as President of both the American
Elasmobranch Society and the Annual Sea Turtle Symposium and has also served
on numerous national and international advisory groups for conservation and man
agement, most recently on the International Union for the Conservation of Nature
working groups for sharks and sea turtles. He has authored or contributed to more
than 100 research papers and 3 books.
Contributors
Ralph A. Ackerman, Ph.D. Catherine M.F. Lohmann, Ph.D.
Department of Zoolology and Genetics Department of Biology
Iowa State University University of North Carolina
Ames, Iowa Chapel Hill, North Carolina
Chapter 2
Population Genetics, Phylogeography, and Molecular Evolution......................... 29
Brian W. Bowen and Stephen A. Karl
Chapter 3
Reproduction in Sea Turtles................................................................................... 51
Jejfrey D. Miller
Chapter 4
The Nest Environment and the Embryonic Development of Sea Turtles............. 83
Ralph A. Ackerman
Chapter 5
Orientation, Navigation, and Natal Beach Homing in Sea Turtles..................... 107
Kenneth J. Lohmann, Blair E. Witherington, Catherine M.F. Lohmann,
and Michael Salmon
Chapter 6
Habitat Utilization and Migration in Juvenile Sea Turtles.................................. 137
John A. Musick and Colin J. Limpus
Chapter 7
Sea Turtle Locomotion: Mechanics, Behavior, and Energetics........................... 165
Jeanette Wyneken
Chapter 8
Foraging Ecology and Nutrition of Sea Turtles................................................... 199
Karen A. Bjorndal
Chapter 9
Age, Growth, and Population Dynamics............................................................. 233
M.Y. Chaloupka and John A. Musick
Chapter 10
Diving Physiology................................................................................................. 277
Molly E. Lutcavage and Peter L. Lutz
Chapter 11
Thermal Biology.....................................................................................................297
James R. Spotila, Michael P. O'Connor, and Frank V Paladino
Chapter 12
Hormones in the Life History of Sea Turtles...................................................... 315
David W. Owens
Chapter 13
Salt, Water, and pH Balance in Sea Turtles......................................................... 343
Peter L, Lutz
Chapter 14
Health Problems and Diseases of SeaTurtles...................................................... 363
Robert H. George
Chapter 15
Human Impacts on Sea Turtle Survival................................................................ 387
Molly E. Lutcavage, Pamela Plotkin, Blair Witherington,
and Peter L. Lutz
Index.......................................................................................................................411
1 Evolution, Phylogeny, and
Current Status
Peter C. H. Pritchard
CO NTENTS
1.1 Introduction....................................................................................... 2
1.2 Marine Turtles................................................................................................... 3
1.3 Historical Classifications of Marine Turtles.....................................................5
1.4 Cladistic Placement of Marine Turtle Groups.................................................7
1.5 Marine Turtle Families..................................................................................... 7
1.6 Familial Definitions.......................................................................................... 8
1.6.1 Cheloniidae........................................................................................... 9
1.6.2 Dermochelyidae.................................................................................... 9
1.6.3 Toxochelyidae............................................................................... 9
1.6.4 Protostegidae........................................................................................ 10
1.7 Dermochelyid Diversity................................................................................... 10
1.8 Cheloniid Diversity...........................................................................................11
1.9 Generic Definitions of Living Cheloniids....................................................... 11
1.9.1 Cheionia...............................................................................................12
1.9.2 Eretmochelys....................................................................................... 12
1.9.3 Lepidochelys........................................................................................ 12
1.9.4 Caretta................................................................................................. 12
1.9.5 Natator.................................................................................................13
1.10 Affinities of Recent Cheloniid Species: Current Questions......................... 13
1.11 Survival Status of Living Cheloniids............................................................ 16
1.11.1 Legal and Formal Status..................................................................... 16
1.11.2 Biological Status................................................................................. 16
1.11.2.1 The Green Turtle, Cheionia mydas..................................... 17
1.11.2.2 The Black Turtle, Cheionia agassizii.................................. 18
1.11.2.3 The Flatback, Natator depressus......................................... 18
1.11.2.4 The Loggerhead, Caretta caretta........................................ 19
1.11.2.5 The Hawksbill, Eretmochelys imbricata.............................20
1.11.2.6 The Olive Ridley, Lepidochelys olivácea............................22
1.11.2.7 The Kemp’s Ridley, Lepidochelys kempi............................22
1.11.2.8 The Leatherback, Dermochelys coriacea............................23
References............................................ ...................................................................24
0-8493-8422-2/97/$0.00+$.50
© 1997 by CRC Press, Inc
The Biology of Sea Turtles
1.1 INTRODUCTION
Turtles, being reptiles and tied to the terrestrial environment for oviposition (with
the single partial exception of the chelid Chelodina nigosa [Kennett, 19931), are
generally assumed to have had terrestrial origins. This assumption is probably
correct. Additional evidence is provided by the earliest fossil turtles of the genus
Proganochelys and its relatives that had already achieved a broad distribution in the
northern continents (then still united as “Laurasia”) by the Triassic, and that appear
from their limb structure to have been terrestrial, or, at most, marshdiving forms
(Gaffney, 1990).
Authors such as Römer (1945) and Swinton (1958) assumed that the group of
early reptiles long known as the Cotylosauria, characterized inter alia by the absence
of true temporal fossae, was ancestral to the turtles. Yet intermediate stages were
unknown in the fossil record, the alleged “missing link,” Eunotosaurus Seeley 1892,
from the Permian of South Africa having now been reinterpreted, and Swinton,
although observing that “the Chelonian skull has many features in common with
that of Diadectid Cotylosaurs,” cautiously concluded that “a direct relationship is
not likely.”
Gaffney (1975) considered the turtles to be the sister group of the Synapsida
plus the Diapsida. But he later revised this opinion (Gaffney and Meylan, 1988),
and argued that the turtles were the sister group to the Diapsida alone. Subsequently,
Lee (1993) conducted a cladistic analysis of the Chelonii and various groups of
primitive amniotes and concluded, on the basis of 16 shared derived features, that
the pareiasaurs, a group of anapsid, large, terrestrial reptiles with short, heavy bodies,
and with large osteoderms possibly anticipatory of the chelonian shell, were evolu-
tionarily close to the earliest turtles. The pareiasaurs were, for a time, cosmopolitan
in distribution, but although they flourished in the Late Permian, they were not
persistent. Lee considered the evidence linking the chelonians to other traditionally
cotylosaurian groups such as the captorhinids and the procolophonoids to be weak.
In view of the terrestrial adaptations of the earliest turtles, it is noteworthy that
the subsequent evolution of the Order Chelonii has seen a remarkable proliferation
of aquatic taxa, whereas terrestriality has been retained (or reachieved?), amongst
modern forms primarily by the single family Testudinidae (with about 40 living
species). Some isolated fossil genera (e.g., Meiolania, Zangerlia — see Gaffney
[1983] and and Mlynarski [1972] for discussion) also appeared to have been terres
trial, as are a few living emydids and batagurids. Yet eleven of the twelve living
families are basically aquatic, although in some cases families that are considered
exclusively aquatic today, such as the Pelomedusidae and the Dermatemyidae, once
had significant terrestrial representation (Wood, 1985; Mlynarski, 1972). The great
majority of the extinct families were entirely aquatic also, as far as is known.
Moreover, even specialized aquatic forms, such as the Trionychidae, appeared very
early in the fossil record (Hutchison, 1982). Furthermore, as far back as the early
Jurassic, a newly described turtle, Kayentachelys, from the Kayenta fonnation of
eastern Arizona, considered to be the sister taxon for all other cryptodires, had a
carapace with all of the morphological features of modern, fully aquatic species
(Gaffney et al., 1987).
Evolution, Phylogeny, and Current Status 3
The successful penetration of aquatic niches by both early and modern turtles
was probably made possible by a remarkable example of preadaptation. Other living
aquatic reptiles, including the sea snakes and other snake species adapted to various
degrees for freshwater environments, marine iguanas, crocodilians, etc., as well as
the extinct ichthyosaurs, swim (or swam) by means of body and tail undulations not
dissimilar to those of typical fishes. Turtles, on the other hand, lost the capacity for
this form of propulsion when they developed the shortened, rigid body form and
corselet that has characterized the group since the Triassic. This body form offered
armored resistance to attack by predators, but the tradeoff was reduced speed and
agility, obliging those terrestrial chelonian species surviving in a world with increas
ingly sophisticated predators to adopt specialized (fossorial, cryptic, insular) life
styles (Pritchard, 1979).
Nonetheless, while the encarapaced, terrestrial body form with columnar, walk
ing-type limbs precluded aquatic locomotion by serpentine undulation, it was
remarkably preadapted for very different modes of propulsion, namely swimming
by means of alternating thrusts of limbs with webbed digits, or simultaneous strokes
with powerful paddlelike forelimbs, and these occurred repeatedly.
The shell required only modest modifications in the transition to aquatic life,
these generally taking the form of an overall lower, more streamlined profile, with
sharp, tapered edges to the carapace margins, and expansion of the plastral lobes,
thus facilitating lateral (swimming) rather than ventral (walking) limb movements
(Gaffney et al., 1987).
Only the limbs required profound modifications, and these were indeed forth
coming, the precise form of the newly reshaped limbs being governed by the degree
of aquatic specialization appropriate to the new life-style. In the vast majority of
aquatic chelonians, the limbs represent a compromise between the needs of swim
ming and of walking. In most swimming fonns the hind limbs are longer, more
powerful, and have more extensive webbing than the forelimbs, and they generate
the principal propulsive force, although their narrow specialization for this function
alone is constrained by the need in all chelonian species to utilize the hind limbs
for nest construction.
But in some of the most aquatic chelonians, the forelimbs provide the principal
propulsive force. A trend in this direction is evident in the soft-shelled turtles
(Trionychidae), in which the forelimbs have become substantially modified into
swimming paddles, and the foreclaws (and hindclaws) are reduced to three on each
limb. In these turtles, propulsive force is generated to a comparable degree by
forelimbs and hindlimbs. Forelimb specialization for swimming is carried further in
the family Carettochelyidae (now monotypic, but with an elaborate fossil record and
forebears widespread in northern continents [Mlynarski, 1976]), in which the fore
limbs are paddlelike and two-clawed, but still flexible.
enmeshed in a continuous matrix of tough connective tissue. This not only makes
independent mobility of individual digits impossible, but flexion of the blade of the
paddle as a whole is tightly constrained by the combination of the fibrous binding
of the digits and nonalignment of the corresponding joints between the individual
phalanges of adjacent digits (Zangerl, 1980). In such forms, the forelimb claws are
essentially rudimentary, reduced to one, or two at most. But the claw on the first
digit has a specialized secondary function in adult male cheloniids, in which it is
enlarged and hooklike, and is used for clasping the anterolateral marginal area of
the female during copulation.
Such limbs are poorly adapted for terrestrial locomotion in that the proximal
parts of the limbs bear almost the entire weight of the animal, but ability to locomote
on land cannot be compromised beyond a certain point because of the inescapable
marine chelonian committment to terrestrial oviposition. On the other hand, the
extraordinary demands for superior swimming ability and endurance made by a
marine life incorporating transoceanic migrations ensure that adaptations for aquatic
locomotion are paramount and uncompromised, whereas terrestrial locomotion can
be allowed to become slow and labored, as long as it is not entirely precluded.
Turtles are an important component of marine ecosystems — primarily tropical
and to a lesser degree subtropical ones, but with one species (Dermochelys coriacea)
showing remarkable adaptations for survival and function in very cold water (Frair
et ak, 1972; Greer et al., 1973). Today, however, only seven or eight species of
marine turtles survive, the majority distributed unevenly through all three tropical
oceans, but with three having relatively restricted distributions (the flatback, Natator
depressus, in northern Australia; Kemp’s ridley, Lepidochelys kempi, in the Gulf of
Mexico and North Atlantic; and the black turtle, Chelonia agassizH, in the eastern
Pacific).
Despite this paucity of species, the living marine turtles are not a relictual group.
They have great economic value as well as extreme vulnerability to mankind, at
least while nesting, and their inclusion on most lists of threatened or endangered
species is a reflection primarily of past overexploitation and current need for better
management rather than to inherently poor adaptation to post-Pleistocene conditions.
The documented great diversity of sea turtle taxa in the past probably derives
from several causes, including on the one hand the relatively good chances of
fossilization of these heavy-boned animals, and on the other hand the disappearance
of entire isolated oceans (such as the Niobrara Sea), once populated by diverse and
remarkable sea turtle species. Moreover, the overall evolutionary history of the group
is paralleled on a mega-scale by the history of life itself (Gould, 1980) or on a
human-economy scale by the reduction in the number of automobile manufacturers
in the U.S. during the last century even as cars have become more, rather than less
popular (Yates, 1996). In these examples, an early blossoming forth of numerous
fundamentally “experimental” types (Pritchard and Trebbau [1984] list 27 entirely
extinct genera in the Cheloniidae alone) became winnowed down, probably through
elimination of overspecialized types with poor response to changing conditions, to
a much-reduced diversity of modem survivors, characterized to a considerable degree
by being less rather than more specialized than their forebears (Zangerl, 1980).
Evolution, Phylogeny, and Current Status
and the Amydae with distinct digits. The same division with different names was
used by Merrem (1820) and Bell (1828), who called the marine species with oarlike
limbs Pinnata, and the species with distinct digits Digitata.
Further refinements followed. It was soon recognized that the limbs of tortoises
were as distinct from those of freshwater species as were the limbs of the marine
forms. Moreover, the leatherback turtle and also the freshwater softshells presented
radical distinctions from the typical chelonian body form. This led to Gray’s (1825)
recognition of five families, the Cheloniadae, the Sphargidae, the Trionicidae, the
Emydidae, and the Testudinidae.
Agassiz (1857) reviewed subsequent early nineteenth century classifications,
remarking sagely that the schema of Brongniart, Ritgen, Wagler, Duméril and
Bibron, and Prince Canino Bonaparte, although utilizing totally different names, had
a great deal in common. It was Wagler (1830), followed by Duméril and Bibron
(1836) who hit upon the character that is considered to be of the most fundamental
importance today, namely the plane of retraction of the neck. This feature not only
reflects in the articulations of the cervical vertebrae, but also correlates with impor
tant skull features (such as the trochlear mechanism for the jaw retractor muscles),
and shell characters (the pelvis fused to both carapace and plastron in the pleurodires,
and the presence of an intergular scute in the pleurodires, but only rarely in the
cryptodires). Many pleurodire groups also show remarkable parallelisms with cryp
todires, for example in the loss of the mesoplastra, as well as in the characteristic
carapacial scute mosaic.
Agassiz also commented that many of the classifications that he had reviewed
assumed (implicitly or explicitly) that the turtle species with oar- or paddlelike limbs
were “inferior” to those with separate digits, which in turn were “inferior” to those
with clublike walking feet. This evaluation of his predecessors’ beliefs was correct,
and indeed the assumption contains some elements of truth if we substitute the word
“primitive” for “inferior.” For example, the marine turtles do show the complete
(nonemarginate) skull roof considered characteristic of early turtles, and the char
acteristic elephantine foot of the testudinids is indeed probably a derived rather than
a primitive feature. But it should not be forgotten that both Agassiz and the ante
cedents whom he quoted were nonevolutionists, and thus concepts of primitive vs.
advanced features, or of “natural groups,” had at most a theological basis rather than
one based upon actual closeness of phylogenetic relationship.
Subsequent classifications, of living turtles at least, were based upon the primary
division of turtles and tortoises into Pleurodires and Cryptodires, although the proper
alignment of the leatherback and the softshells remained a challenge. The many
morphological peculiarities of the former (summarized by Gervais, 1872) led Cope
(1872) to declare this taxon the “sister group” (to use cladistic terminology) for all
other turtles. He named it “Athecoidea” (or, in family-level nomenclature, “Athe
cae”), meaning “without a shell.” This allocation was followed by Dollo (1886),
Boulenger (1889), Lydekker (1889), and various others, the latest author of note to
use it being Carr (1952). In these classifications, all other chelonians were identified
as Thecophora (“shell-bearing ones”).
Furthermore, the Thecophora were trichotomously divided into the superfamilies
Cryptodira, Pleurodira, and Trionychoidea (Boulenger, 1889). Such elevation of
Evolution, Phylogeny, and Current Status 7
status of the taxa with scuteless shells is understandable, in that it makes a mockery
of classifications founded upon details of scutation when some turtles have no scutes
at all. But, despite their extremely divergent morphology, Dermochelys and its fossil
relatives are now considered to be related to the marine turtles of the family Che-
loniidae (Gaffney and Meylan, 1988).
Yet Cope’s early (1872) designation of a taxon (Athecoidea) linking the pro-
tostegids and the dermochelyids, while shaken by Zangerl’s subsequent clarification
that protostegids (in contrast to demiochelyids) had normal chelonian scutes rather
than leathery skin, achieved some vindication with Gaffney and Meylan’s (1988)
proposal of an “Epifamily” Dermochelyoidae, with the same content. Moreover,
even Dollo, while recognizing the Athecae on the basis of its extreme morphological
modifications, considered it to be derived from the Cheloniidae (Dollo, 1886).
Dollo’s conclusion may be factually correct, although the divergence was so far back
that it is arguable whether or not the common ancestral stock was truly cheloniid.
But his nomenclatural inteipretation of its implications, although permissible by
classical taxonomists, is in conflict with the rules of Cladism, which seeks to establish
a nomenclature based purely upon temporal order of divergence rather than upon
degree or extent of divergence.
Today the trionychids also are recognized only as a family, related to the caret-
tochelyids, and incorporated within the Cryptodira (Meylan, 1987).
Various earlier, intermediate, or conflicting placements of the Dermochelyidae
are discussed by Pritchard and Trebbau (1984).
parallels (or anticipations) with later marine turtle families, but the limbs remained
unspecialized for marine life (Hay, 1908), and Gaffney and Meylan (1988) recently
found little or nothing to separate Thalassemys itself from the plesiochelyids. Cer
tainly, Hay’s concept of the Thalassemyidae was a polyphyletic one, with his family
definition following rather than preceding the allocation of a mixed bag of genera
from at least three distinct families.
Some extinct Pelomedusid genera (e.g., Taphrosphys [Wood, 1974]; possibly
Stupendemys [Wood, 1976]) may also have been marine.
By the Cretaceous, four marine turtle families — the Toxochelyidae, Protoste-
gidae, Cheloniidae, and Dermochelyidae —were all established, the last two surviv
ing to the present. Whether or not these all represent independent invasions of the
marine environment is unclear, but it is probable (as Dollo observed) that the
dermochelyids represent an extraordinarily divergent offshoot of the cheloniids,
whereas some of the toxochelyids bear such a strong superficial resemblance to
certain cheloniids that the possibility of close relationship must be seriously enter
tained.
based upon purely osteological features. Definitions for the entirely extinct marine
families are necessarily less complete.
1.6.1 C heloniidae
1.6.2 D ermochelyidae
A family of turtles characterized by extreme reduction of the bones of the carapace
and plastron (with the neural and peripheral bones of the carapace, and entoplastron
of the plastron, lacking; the pleurais reduced to endochondral ribs, separated by
wide fenestrae; and the plastral bones reduced to narrow splints, forming a ring of
bones surrounding a great fontanelle); development of a neomorphic epithecal shell
layer consisting of a mosaic of thousands of small polygonal bones; claws and shell
scutes lacking (scales only present in the first few weeks of life); skull without nasal
bones; jaw surfaces covered with keratin, but lacking differentiated rhamphothecae;
parasphenoid overlain by pterygoids; prefrontals in contact dorsally, with descending
processes that are moderately separated; unridged tomial surfaces; a generally neo-
tenic and oil-saturated skeleton; extensive areas of vascularized cartilage in the
vertebrae, limb girdles, and limb bones; very large body size; and marine habitat.
1.6.3 T oxochelyidae
The toxochelyids were a group of small to medium-sized, broad-shelled (circular
to cordiform) marine turtles that diverged from the early cheloniid stock in the early
Cretaceous and persisted in the proto-Atlantic and Niobrara seas until the late
Eocene. Various toxochelyid genera display progressive development of a secondary
palate, from Toxochelys with a primary palate only, through Ctenochelys with
incipient undershelving, a more advanced condition in Osteopygis, and extreme
development of the secondary palate in Erquelinnesia. The carapace ranged from
fully closed (i.e., without intercostal fontenalles) in Osteopygis to a highly reduced
condition with persistent, large fontanelles in Erquelinnesia. Features characteristic
of the family include the presence of small nasal bones and the well-developed
10 The Biology of Sea Turtles
1.6.4 Protostegidae
The protostegids were a group of large to gigantic turtles whose more primitive
members (e.g., Rhinochelys) have been found in Cretaceous deposits of France and
England, but which reached its maximal development (with the genera Protostega
and Archelon, known from Upper Cretaceous deposits of Texas, South Dakota,
Arkansas, Alabama, Colorado, and Kansas) in the Niobrara Sea. They showed certain
parallels with the dermochelyids, one of these being the possession of vascularized
chondroepiphyses (Rhodin, 1985), a feature present in a number of fossil turtle
genera (Psephophorus, Eosphargis, Archelon, Pneumatoarthrus, Corsochelys) as
well as the living Dermochelys. However, vascular chondroepiphyses, a condition
readily evident in the ends of the limb bones even of purely fossil material, may be
a parallelism connected with very large adult size (Pneumatoarthrus was originally
described as a dinosaur! [Cope, 1870; Baird, 1978]), and rapid growth potential
rather than an indicator of relationship.
The protostegids were characterized by large skulls (up to 100 cm in length in
the case of Arche Ion) with well-developed, sometimes strongly hooked beaks; no
secondary palate; reduced xiphiplastra and reduced or absent epiplastra; considerable
reduction of the carapacial armor, in some cases with intercostal fontanelles reaching
close to the vertebral column even in adults; a tendency towards keeled or tuberculate
middorsal keels (sometimes with carination present only on alternating neural
bones); and a slight to moderate degree of temporal emargination.
from several mostly complete specimens. In this genus, the shell is characterized by
thickened, strongly keeled epithecal bones that form a complete layer on the plastron
as well as the carapace (in contrast to Dermochelys).
In the genus Eosphargis (Eocene, Europe), a well-preserved skull shows typi
cally dermochelyid foim, but the mosaic epithecal layer was not yet developed
(Nielsen, 1959).
characters, that would permit correct diagnosis and allocation of a new potential
congener, fossil or living, that was unknown when the diagnosis was drawn up.
The diagnoses of Carr (1942) for four of the known cheloniid genera meet these
criteria, and are quoted below with very minor modifications. On the other hand,
the two recently presented and much more detailed diagnoses for Natator (Zangerl
et al., 1988; Limpus et al., 1988) are indistinguishable from diagnoses of the single
species N. depressus, in that they combine both fundamental (generic-level) and
species-specific data.
1.9.1 C helonia
One pair of prefrontal scales; homy cutting edge of lower jaw coarsely dentate, that
of upper Jaw strongly ribbed vertically; bony alveolar surface of upper jaw with a
low, but regularly raised auxiliary ridge behind the anterior ridge which is very
strong and terminates anteriorly in a pointed eminence at the posterolateral corner
of the premaxillary pit; costal scutes 4 pairs, well cornified, juxtaposed.
1.9.2 Eretmochelys
Two pairs of prefrontal scales; nuchal scute not in contact with first costáis; snout
elongate, narrow, the mandibular symphysis deeply excavated and not terminally
toothed; bony alveolar surface of upper jaw with a sharp-crested ridge; dorsal scutes
usually 4 pairs, usually thick and conspicuously imbricated; peripheral bones usually
11 (after Carr, 1942).
1.9.3 L epidochelys
Maxillaries not in contact, separated by vomer; frontal bone usually entering rim of
orbit; pterygoids markedly broadened anteriorly, the ectopterygoid processes strong;
fontanelles in choanal chamber near opening, not hidden by alveolar surface in
ventral aspect; external openings of orbits not concealed by overlying bones in
ventral aspect; descending processes of prefrontals not reaching palatines; lower jaw
with a more or less sharp and strong median elevation at the posterior border of the
bony alveolar surface, which may or may not extend forward as an elevated ridge;
four enlarged inframarginal scutes on the bridge; costal scutes thin, juxtaposed, 5
to 9 pairs (often asymmetrical); neural bones 11 to 15.
1.9.4 C aretta
Maxillaries in contact, not separated by vomer; pterygoids not, or but slightly,
broadened anteriorly, the ectopterygoid processes vestigial or lacking; fontanelles
far forward in choanal chamber, completely concealed by alveolar surface in ventral
aspect; external openings of orbits concealed by overlying bones in ventral aspect
(in mature specimens); descending processes of prefrontals connected with palatines;
bony alveolar surface of lower jaw smooth at posteromedian border; symphysis of
mandibles without longitudinal ridge; bridge with three enlarged inframarginal
Evolution, Phylogeny, and Current Status 13
scutes; costal scutes well comified, juxtaposed, usually five pairs; neural bones
usually 7 or 8.
1.9.5 N atator
Maxillaries widely separated by vomer; pterygoids broadened anteriorly and with
very strong ectopterygoid processes; fontanelles far forward in choanal chamber,
completely concealed by alveolar surface in ventral aspect; external openings of
orbits not concealed by overlying bones in ventral aspect; symphysis of mandible
with a strong median ridge terminating posteriorly in an elevated prominence;
external openings of internal carotid canals very large; head scalation characterized
by a pair of preoculars lying between maxillary rhamphotheca and prefrontals, post-
parietals variable and often asymmetrical, generally including an undivided median
element; plastral fontanelles closing at sides, but remaining open medially at matu
rity; four pairs of inframarginal scutes; carapacial scutes very thin, poorly kerati
nized, and with sulci tending to disappear with age; costáis four pairs. Neural bones
about twelve, with frequent transverse division of “standard” elements, and 2 or 3
suprapygals.
From the above diagnoses, the confusing “mosaic” of characters of Natator is
evident. The skull is so similar to that of L. kempi that some of the older skulls in
Australian museums have been identified and catalogued as that species.
1969). However, earlier workers lacked the material to make these comparisons, and
the two were placed in separate genera {Colpochelys and Lepidochelys) for a long
time. Carr (1942) had to report that prevailing opinion even then insisted either that
the two ridleys and the loggerhead should each be in separate genera, or that all
three forms should be united within Caretta. Yet, he added, it “seems evident that
kempii should be associated with olivácea in the genus Lepidochelys Fitzinger,” an
allocation presaged by Baur (1890) and followed by most subsequent authors (see
Pritchard, 1969, 1989 for details).
But there were holdouts, the most prestigious being Loveridge and Williams
(1957) and Mertens and Wermuth (1955), who considered kempi to be merely a
subspecies of L. olivácea. Once again, Bowen et al. (1993) offered a mitochondrial
DNA analysis, in this case demonstrating that, although the two forms were sister
taxa, they showed genetic distinction at the species level.
The “black turtle” was described as a full species, Chelonia agassizii, by Bocourt
(1868). C. mydas caninegra Caldwell 1962, appears to be a synonym, although the
assumption that this forni from the Gulf of California is identical to agassizii from
Pacific Guatemala has not been proven (but is probable). The pros and cons of
species vs. subspecies status for agassizii have been discussed by Mrosovsky (1983)
and Pritchard (1983). I have justified my own position (full species status) on
narrowly technical grounds (i.e., the nomenclature of the original describer, Bocourt,
should stand until formally changed), and on biological ones, in that agassizii and
mydas-Y\kç^ forms are sympatric in several places in the Pacific, including the Gal
apagos islands and New Guinea; the degree of differentiation in size, shape, and
color is more extreme than that found in any other Chelonia population; the dark
plastral coloration of agassizii is not environmentally derived; and there may be
purely physical reasons why a 40-kg adult male agassizii may be unable to mate
successfully with a 200-kg adult female mydas, even if the two do come in contact.
Subsequent contributions on the subject include that of Kamezaki and Matsui
(1995), who analyzed geographic variation in skull morphology of six Chelonia
populations (two from each tropical ocean), and concluded that the Galapagos
sample (i.e., agassizii) was completely separated from the other samples by a
canonical discriminant analysis, and that this result indicated distinctness of the
eastern Pacific population. On the other hand, Bowen et al. (1993) found that eastern
Pacific turtles represented but a small subset of lineage diversity within the broader
and deeper mtDNA gene tree for the globally distributed green turtle.
A conclusive decision on this issue thus remains elusive. Bowen et al. found
that nDNA data indicated very close relationship between the Galapagos and the
Pacific Mexican populations of "'agassiziC, but both lacked close affinities with
south-central and western Pacific populations, which argues in favor of species-
level status for agassizii. On the other hand, a phylogeny derived from nDNA links
the eastern Pacific populations with, inter alia, those of Ascension Island and the
Atlantic Coast of Africa (Parham and Zug, 1996)! This discordance of nDNA with
both morphology and geographic probability would, to some, substantially lower
the credibility of nDNA analysis as a technique for elucidating phylogenetic
relationships.
16 The Biology of Sea Turtles
The U.S. Department of the Interior, under the authority of the Endangered Species
Act, currently lists C. mydas (endangered in Florida and Pacific Mexico) and Caretta
caretta as Threatened Species. E. imhricata, L. olivácea, L. kempi, and Dermochelys
coriácea are listed as endangered. N. depressus, the most restricted and least-known
of all living cheloniids, is unlisted.
The Convention on International Trade in Endangered Species of Flora and
Fauna (CITES) lists all marine turtles on its Appendix I (i.e., prohibited from
international trade from or to signatory countries).
The World Conservation Union (lUCN) has listed C. caretta as Vulnerable and
all other sea turtle species (except A. depressus) as Endangered. However, the lUCN,
in close cooperation with the Secretariat and Parties to CITES, has now adopted a
set of complex numerical and ostensibly objective criteria by which the status
category of a species should be deduced. While these criteria are expected to apply
only to species proposed for the CITES appendices in the future, they will be
incorporated across the board by lUCN. The criteria incorporate considerations of
actual global population numbers, fragmentation of habitat and populations, and
demonstrable population trends. For the great majority of species, the necessary data
are unlikely to be currently available. Among the sea turtles, even the “critically
endangered” L. kempi may only qualify as “conservation dependent,” in view of its
modest population recovery in recent years.
St",
'■ iT ^ u ‘
Thus, it remains to be seen whether the Tortuguero, Costa Rica, nesting colony
of the green turtle, although still large and the most important by far in the Caribbean,
can indefinitely survive heavy subsistence take by Costa Ricans from the city of
Limon as well as by Miskito people from Caribbean Nicaragua, San Bias people
from Panama, and Guajiros from northeastern Colombia and northwestern Venezu
ela. Similar uncertainties are faced by the large Australian colonies, protected whilst
18 The Biology of Sea Turtles
in Australian territory and waters, but heavily harvested in various islands of Indo
nesia (Limpus, 1994, 1995).
Here and there, green turtle colonies seem to be on the increase. In the last
decade, not only has regular green turtle nesting been observed where none was
seen before at Rancho Nuevo, Tamaulipas, Mexico, but also, on the mid-Atlantic
coast of Florida, U.S.A., green turtle nesting is now commonplace on beaches where
almost nothing but loggerheads nested two decades ago. These increases on well-
patrolled beaches seem to be real, and with good protection now offered to many
nesting colonies, including those of Atol das Rocas, Ascension Island, and Trindade,
the green turtle does not appear to be faced with imminent extinction.
Parenthetically, it is interesting to note that proponents of turtle farming and
ranching have observed that, although the green turtle has been sought by interna
tional gourmets for centuries, it is no more endangered than other sea turtle species,
and thus commercial utilization could not have been a significant factor in any
decline that the species may have experienced (Fosdick and Fosdick, 1994).
This species is unlisted by the authorities of USDI and lUCN because it is essentially
confined to the waters of a single nation (Australia), where it is protected by law
except from aboriginal harvest, and where most of the nesting beaches are remote
and undeveloped (with the exception of the easily accessible Mon Repos Beach in
Queensland). Moreover, Australian native people in general prefer to consume the
more abundant and more succulent C. mydas. The outlook for Natator would thus
appear to be good, but the vulnerability of individuals to incidental capture in prawn
trawls, and the overall relatively restricted range lending panspecific vulnerability
from single catastrophic environmental events, leave no grounds for complacency.
Evolution, Phylogeny, and Current Status 19
terranean, and southern Brazil, but it also brings the species into contact with
industrial and development stresses ranging from massive incidental capture in
Atlantic shrimp trawls to resort and recreational development of nesting beaches.
At present, it appears that nesting populations are declining as a result of incidental
catch in both southern Queensland, Australia (Limpus, 1994) and in the U.S. north
of Cape Canaveral, but on the other hand the larger populations in Florida south of
Cape Canaveral and also the relatively small population in Natal, South Africa, are
increasing (National Research Council, 1990).
Intense national and international trade in hawksbill shell products and, in more
recent decades, entire stuffed hawksbill turtles, has led to widespread concerns that
the species is being seriously overexploited. Moreover, although this may be the
most commonly seen species in certain tropical reef habitats in the Caribbean Islands
or tropical Australia, such populations generally consist primarily of subadults, and
very few nesting “colonies” (as opposed to isolated nesting females) are known.
Certain!; it is fundamentally less abundant than the green turtle, but downward
The green turtle
t i* Chelonia m ydas
"'J' '.'T i
The flatback
Natator depressus.
The loggerhead
Caretta caretta
The hawksbill
Eretm ochelys im bricata
Olive ridley
Lepidochelys oUvacea
Kemp’s ridley
Lepidochelys kern p i
The leatherback
D erm ocheiys coriacea
Evolution, Phylogeny, and Current Status 21
trends are often difficult to determine from available data, and some of the more
dire warnings about imminent extinction of the species have stemmed simply from
the contrast between the very substantial global harvest for shell and other products,
and the modest size of the known nesting populations. In a global analysis of the
status of the hawksbill, Groombridge and Luxmoore (1989) could only classify it
as “indeterminate.”
For many decades, Japan has constituted the largest importer of raw tortoiseshell.
However, Japan has now withdrawn its reservation to this species on the CITES
convention, where it is listed as Appendix I (banned in international commerce),
and has phased out importation of hawksbill products. On the other hand, Cuba —
a major habitat for the hawksbill and supplier of world tortoiseshell markets — has
recently expressed the opinion that its domestic hawksbills not only constitute a
nonmigratory stock, but also have a sufficiently healthy population to justify down
grading to CITES Appendix II. The intention is to develop hawksbill ranches in
Cuba to supply the Japanese demand. The implications of this move are ambiguous,
but the conditions for approved commercial ranching of Appendix II species agreed
by the Parties to CITES in November 1994 are sufficiently demanding that a legal
Cuban turtle ranching program may well be linked to much better protection of
Cuban, and regional, hawksbill stocks than has hitherto been available.
In a review of worldwide population trends of the hawksbill, Limpus (1995)
could only find one example of an increasing hawksbill population — that of the
Sabah Turtle Islands (Malaysia), where a more than tenfold increase has been logged
since 1969 (Trono, 1994).
22 The Biology of Sea Turtles
There is some evidence that major “arribadas” are not permanent phenomena,
but rather they may wax and wane over cycles of at least several decades. Several
Mexican arribadas have disappeared in recent decades, and numbers are dropping
rapidly at the protected Nancite site in Costa Rica. Furthermore, the recent discovery
of a new site in Orissa, India, may correspond to the collapse of one of the two
previously documented arribadas along this coast. A former minor arribada in Suri
nam (the only one in the Atlantic) has now been decimated, presumably by a
combination of heavy egg collection for several decades and ongoing trawler mor
tality.
only known nesting site for this species. However, no subsequent data were available
from Rancho Nuevo until 1965, by which time the biggest arribada numbered less
than 5000 turtles, and despite beach protection this number continued to drop for
the next 20 years, by which time total nestings for the season numbered in the
hundreds only. Such nonresponse to protection is a classic indication of a population
for which recruitment has been nullified by near-total harvest of eggs, in this case
exacerbated by the intensity of shrimp trawling in the Gulf of Mexico (the primary
juvenile and subadult habitat and the only habitat of adult L. kempi) and, during at
least one episode in the late 1960s, beach slaughter of adults also (P. Burchfield,
personal communication). The nesting population reached a low point in the mid-
1980s, and has subsequently increased modestly, but steadily, possibly as a result
of the “head-starting” program initiated in 1978, about 10 years (thought to corre
spond to the approximate maturation time of the species) after the commencement
of the head-starting program. The species remains the rarest sea turtle in the world,
but the current apparent annual population increase may identify it more as “con
servation dependent” than actually “endangered.”
i |l ® i
None of the major nesting grounds of this species were discovered before the 1950s,
and many of them only in the 1960s or 1970s, and thus it is impossible to compare
contemporary population estimates with those of earlier in the century. Pritchard
(1982) noted recent discoveries of major nesting grounds in Mexico, and summarized
preceding discoveries that led to progressively increasing world population esti
mates.
The leatherback does not feature in international commerce, and its juvenile
stages (indeed, all stages between hatchling and adult) remain so cryptic that it is
24 The Biology of Sea Turtles
unlikely that humans have any effect upon them. On the other hand, subsistence
take of eggs, and sometimes of nesting adults also, has been intense, especially in
the Eastern Pacific and Guyana, and while Asiatic nesting colonies (such as that in
Terengganu, Malaysia) are generally exploited for eggs rather than meat, this can
be equally devastating.
At present, the Atlantic colonies (especially in Trinidad, Suriname, and French
Guiana) appear to be reasonably secure and even increasing, as is the small nesting
colony in Natal, South Africa, and adjacent Mozambique. On the other hand, the
Terengganu colony has collapsed in recent years, and serious declines have been
documented in Pacific Mexico and Costa Rica, a result of the combination of beach
slaughter, egg collection, and serious incidental captures by fishing gear in the open
sea. Spotila et al. (in press) speculate that the species may be threatened with actual
extinction within the forseeable future, although Pritchard (in press) disagreed.
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Brown, Boston, 1857, 452 p.
Baird, D., Pneumatoarthrus Cope, 1870, not a dinosaur but a sea turtle, Proc. Acad. Natl.
Sci. Philadelphia, 129, 71-81, 1878.
Baur, G., The genera of the Cheloniidae, Am. Nat., 24, 486-487, 1890.
Bell, T, Characters of the order, families, and genera of the Testudinata, J. Zool, 3, 513-516,
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Bocourt, M.-M., Description des quelques chéloniens nouveau appartenant à la faune mex
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Garman, S., On certain species of the Chelonioidae,ßw//. Mus. Comp. Zool., 6 , 123-126, 1880.
Gervais, R, Nouv. Arch. Mus. Hist. Nat. (Paris), 8, 1872.
Gould, S. J., An early start, in The Panda’s Thumb. More Reflections in Natural History,
Norton, New York, 1980, 217-226.
Gray, J. E., A synopsis of the genera of reptiles and amphibia with a description of some new
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Greer, A. E., Lazell, J. D., and Wright, R. M., Anatomical evidence for a counter-cuiTent heat
exchanger in the leatherback turtle (Dermochelys coriacea), Nature, 244, 181, 1973.
Groombridge, B. and Luxmoore, R., The green turtle and hawkshill (Reptilia: Cheloniidae):
world status, exploitation and trade, CITES, UNEP, 1989, 601 p.
Haworth, A. H., A binary arrangement of the Class Amphihm, Philos. Mag. (London), 65,
372-373, 1825.
Hay, O. P., The fossil turtles of North America, Carnegie Inst. Washington PubL, 75, 1-568,
1908.
Hutchison, J. H., Turtle, crocodilian and champsosaur diversity changes in the Cenozoic of
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Kennett, R., Of droughts and flooding rains and a turtle that nests underwater. Second world
Congress of Herpetology, Abstracts, 1993, 138-139.
Klein, Quadrup. Disp. Lipsiae 1751. (not seen; cited by Agassiz, 1857).
Lee, M. S. Y, The origin of the turtle body plan: bridging a famous morphological gap.
Science, 261, 1716-1720, 1993.
Limpus, C. J., Marine conservation. Marine turtles: ancient mariners in distress. Air Sea
Rescued. (Australia), 12(2), 99-100, 103, 105, 106-113, 1994.
Limpus, C. J., Global overview of the status of marine turtles: a 1995 viewpoint, in K.
Bjorndal, Ed., Biology and Conservation o f Sea Turtles, rev. ed., Smithsonian Institution
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Limpus, C. J., Gyuris, E., and Miller, J. D., Reassessment of the taxonomic status of the sea
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Stockholm, 1758, 823 p.
Loveridge, A. and Williams, E. E., Revision of the African tortoises and turtles of the suborder
Cryptodira, Bull. Mus. Comp. Zool., Harv. Univ., 115, 163-557, 1957.
Lydekker, R., Catalogue of the Fossil Reptilia in the British Museum (Natural History), Part
3, Chelonia, British Museum (Natural History), London, 1889, 239 p.
McCulloch, A. R., A new genus and species of turtle from North Australia,/?cc. Austr. Mus.,
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Merrem, B., Tentamen Systematis Amphibiorum, Marburg, Germany, 1820, 191 p.
Mertens, R. and Wermuth, H., Die rezenten Schildkröten, Krokodile und Brückenechsen,
Zool. Jahrb. (Syst.), 83, 5, 323-440, 1955.
Meyer, F. I. C., System des Thierreichs, etc., Verh. Naturforsh Ver. Rheinland, 1949.
Meylan, R, The phylogenetic relationships of soft-shelled turtles (family Trionychidae),5w//.
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Williams, E. E., Grandison, A. G. C., and Carr, A. F., Chelonia depressa Garman re-investi
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2 Population Genetics,
Phylogeography, and
Molecular Evolution
Brian W. Bowen and Stephen A. Karl
CONTENTS
2.1 Introduction...................................................................................................... 30
2.1.1 Technical Considerations.................................................................... 30
2.2 Molecular Systematics and Evolution............................................................ 31
2.2.1 D erm ochelys coriácea: an Ancient Lineage?................................. 32
2.2.2 E retm ochelys im bricata: the Origin of an Unusual
Dietary Habit....................................................................................... 33
2.2.3 N atator depressus: a Recent Taxonomic Reassignment....................33
2.2.4 L epidochelys kem pii and L. olivácea: Biogeography and
Conservation....................................................................................... 34
2.2.5 C helonia m ydas and C. agassizi: When is a Green Turtle not
a Green Turtle?................................................................................... 35
2.2.6 Marine Turtle Hybrids: the Oldest Bastards Known
to Science............................................................................................ 36
2.3 Green Turtle Population Structure.................................................................. 38
2.3.1 Maternal Genetic Markers: a Test of Natal Homing.........................38
2.3.2 Nuclear vs. Mitochondrial DNA: Contrasting Population
Genetic Structures within an Organismal Lineage............................38
2.4 Loggerhead Turtle Population Structure........................................................40
2.4.1 Regional Population Structure: the Southeast U.S.............................40
2.4.2 Feeding Ground Composition............................................................40
2.5 Hawksbill Turtle Population Structure...........................................................43
2.5.1 Population Genetic Structure: the Geographic
Scale of Nesting Populations..............................................................43
2.5.2 Genetic Markers and International Conservation..............................44
2.6 Summary......................................................................................................... 45
References................................................................................................................46
0-8493-8422-2/97/$0.00+$ 50
© 1997 by CRC Press, Inc 29
30 The Biology of Sea Turtles
2.1 INTRODUCTION
The age of biotechnology is upon us, and even the most venerable branches of
natural history have been sculpted by the advancing tide. Although the application
of genetic analyses to marine turtles is a relatively recent undertaking, research
efforts have already proven informative. The success of genetic studies is attributable
in part to the paucity of information on marine turtle biology prior to the last decade.
In a group of little-known animals, relatively simple genetic observations (such as
a comparison of DNA sequences in putative sister species) may constitute substantial
scientific advances.
Molecular genetic assays have special attributes which are particularly well
suited for examining cryptic components of marine turtle life history and evolution.
The DNA in each cell can provide genetic markers to identify individuals, popula
tions, species, and higher taxa. In addition, DNA sequence comparisons can illumi
nate the evolutionary history of sea turtles, as well as aspects of reproductive behavior
and ecology. For example, do female sea turtles return to nest on their natal beaches?
A maternal genetic marker such as mitochondrial DNA (mtDNA) is appropriate for
tracing the dispersal of female lineages.^ Do males return to their natal rookeries,
or do they mate with females from other nesting beaches? Analysis of nuclear DNA
(nDNA) fragments can reveal the level of gene flow mediated by males. Does more
than one father contribute to a clutch? Microsatellite and minisatellite DNA analyses,
popularly known as DNA fingerprinting, can resolve the paternal contribution to a
cluster of eggs. How distinct is the nominal black turtle, Che Ionia agassizi, from
the green turtle, C. mydasl Because DNA sequences diverge at a more or less
predictable pace over evolutionary time scales, they are useful for reconstructing
phylogenetic relationships, especially for taxonomic groups which are difficult to
distinguish morphologically. The slow pace of morphological evolution in marine
turtles makes the application of molecular systematics especially useful for these
animals.
Molecular studies of marine turtle systematics have advanced beyond prelimi
nary stages, and now include nDNA as well as mtDNA phylogenies. Studies of
several of these species are currently underway, and many of the gaps in our
knowledge will be bridged in the next decade as research programs on five continents
have begun to apply molecular genetic assays to facets of marine turtle biology.
genome consists of tens of thousands of genes, and identifying the same gene from
scores of individuals was an imposing technical challenge. Initially, this problem
was circumvented by working with the extranuclear mitochondrial genome. The
mtDNA of vertebrates is about 16,000 to 17,000 nucleotides, a convenient size to
assay with restriction endonucleases. mtDNA also is maternally inherited in verte
brates, which allows the elucidation of gender-specific behaviors (such as female
nest site fidelity).
One of the most powerful and exciting additions to the molecular genetic
toolbox in recent years is the polymerase chain reaction (PCR).^-^ This process
allows researchers to synthesize millions of copies of a particular segment of DNA
from only a few starting molecules. Pieces of either the mitochondrial or nuclear
genome can now be efficiently amplified and characterized. The PCR process can
be applied to minute pieces of DNA-containing tissue such as a hair follicle, a toe
clip, or a single drop of blood. Furthermore, PCR techniques do not require fresh
tissue; samples can be preserved in ethanol, detergent-based preservative, or a
saturated salt (NaCl) buffer.^’^ With this approach, large numbers of individuals
from natural populations can be sampled relatively easily and without causing them
harm.
One of the fastest evolving segments of the mtDNA molecule is the control
region (sometimes referred to as the D-loop). This region is not a gene coding for
a protein, but rather the site of the origin for replication of the mtDNA molecule.
The relatively rapid rate of mutation allows fine-scale resolution of populations, and
for this reason control region DNA sequencing has become the method of choice
for population definition with mtDNA.^
Fine-scale population discrimination can also be accomplished with a relatively
new class of hypervariable nDNA m arkers.^T hese nDNA regions, often termed
microsatellite loci, generally consist of a region of repeated pairs, triplets, or qua
druplets of DNA nucleotides (such as GTGTGTGT... or AACAACAAC...). Undoubt
edly, this approach will prove valuable for the assessment of fine-scale population
structure and male-mediated gene flow in marine turtles.
mtDNA PHYLOGENY
nDNA PHYLOGENY
*C. CARETTA
L. KEMPIl
L. OLIVACEA
>E. IMBRICATA
N. DEPRESSUS
C. MYDAS
D. CORIACEA
C. SERPENTINA
FIGURE 2.1 Phylogenetic relationships among marine turtles based on mtDNA'^ and
anonymous nDNA sequences.'^ Phylogenetic relationships were inferred by parsimony criteria
(PAUP, version 3.0.5)^'' and statistical support was evaluated by bootstrap resampling. Boot
strap values expressed as percentages are indicated at the nodes.
Until 1988 the flatback turtle was labeled C helonia depressa and was considered a
close relative of the green turtle, C. m ydas. In a taxonomic reassessment based on
morphology and biochemical evidence, Limpus et resurrected the genus N atator
34 The Biology of Sea Turtles
for the flatback turtle. Zangerl et al.^‘ went further in invoking a third tribe within
Cheloniidae, Natatorini, for this species.
The mtDNA sequence data support the genus-level distinction of the flatback
turtle (N. depressus), and are not consistent with the earlier assignment to the genus
Chelonia}^'^'^ An expanded mtDNA data set indicates that flatback lineage is more
closely related to the Carettini than the Chelonini.^^ The nDNA data do not fully
resolve the phylogenetic position of N. depressus. Sequence data from additional
nuclear loci would be desirable to clarify this situation.
Over the last century, the Kemp’s and olive ridleys have been alternately described
as subspecies, species in the genus L epidochelys, or members of the loggerhead
genus Carettad^^^^ The two ridley forms are readily distinguished from each other
by coloration, but no external morphological character will unambiguously distin
guish these sister taxa. Hence some question exists regarding the evolutionary
distinctiveness of the Kemp’s ridley.
In the first thorough examination of ridley evolution, Pritchard^^ suggested that
progenitors of the Kemp’s and olive ridleys were initially isolated by the Central
American land bridge, now known to be 3 million to 4 million years old.^Minder
this scenario, the olive ridley may have evolved in the Indian-Pacific Ocean and only
recently invaded the Atlantic Ocean via South Africa. Pritchard^^ concluded that the
two forms merit species-level distinction, based on morphology and osteology.
However, this taxonomic decision required careful deliberation because of the overall
moiphological conservatism of the genus.
Both mtDNA and nDNA sequence data support the evolutionary distinctiveness
of the Kemp’s ridley. The levels of divergence between L. kem pii and L. olivácea
are consistent with the species-level assignments recommended by Pritchard:^^
mtDNA divergence between ridley forms is greater than the divergence observed
within other species of marine turtle^^’^"^ (but see Reference 17). These genetic data
also provisionally support the biogeographic scenario proposed by Pritchard.^^ Olive
ridleys in the Atlantic and Pacific oceans are closely related in terms of mtDNA
sequences and are divergent from Kemp’s ridley at approximately the level expected
for 3 million to 4 million years of isolation.
The DNA sequence data alone cannot indicate whether or not L. kem pii and L.
olivácea are good species. Nonetheless, the results of both the mtDNA and nDNA
studies indicate that these two groups are genetically distinct at a level which
indicates a long evolutionary separation. This genetic distinctiveness reinforces
Pritchard’s^^ conclusion that the two ridley forms are unique evolutionary units
meriting species-level designations. Accordingly, genetic data bolster the rationale
for intensive conservation efforts for L. kem pii.
Population Genetics, Phylogeography, and Molecular Evolution 35
POLYNESIA (n=2)
AUSTRALIA 100%
JAPAN 5%
JAPAN 70%
JAPAN 25%
HAWAII 73%
HAWAII 27%
GALAPAGOS 100%
PAC. MEXICO 100%
OMAN 100%
(POLYNESIA (n=1)
VENEZUELA 12%
COSTARICA 100%
FLORIDA 88%
FLORIDA 12%
( CYPRUS 100%
VENEZUELA 88%
SURINAM 100%
ASCENSION 3%
ASCENSION 97%
BRAZIL 94%
BRAZIL e%
WEST AFRICA 100%
r
0.8
”T
0.6
"T
0.4 0.2
n
0.0
SEQUENCE DIVERGENCE (%)
FIGURE 2.2a Phylogenetic tree summarizing the relationships among fourteen haplotypes
observed in a mtDNA RFLP survey of green turtle nesting colonies (n = 226)?*^ The geographic
location(s) and the frequencies of each haplotype at these locations are indicated to the right.
Haplotype frequencies for Polynesia were omitted due to small sample size (n = 3).
FIGURE 2.2b Phytogeny summarizing the relationships among 18 iiDNA genotypes from
across the range of Chelonia (1446 nucleotides from loci CM-12, CM-14, and CM-45). This
tree represents a consensus of 171 equally parsimonious arrangements, with the frequency
of each node in these trees indicated as a percentage. No clear distinction is observed between
Atlantic-Mediterranean and Indian-Pacific lineages. However, both nDNA and mtDNA trees
contradict arguments for the evolutionary or taxonomic distinction of the black turtle.
turtles. The combination of the nDNA and mtDNA evidence indicates that these
hatchlings were first-generation hybrids between a loggerhead female and a green
turtle male. Three other occurrences of natural hybridization also have been docu
mented with molecular genetic techniques — a hybrid between a loggerhead female
and a hawksbill male was found in Florida; a Kemp’s ridley female/loggerhead male
hybrid discovered in Chesapeake Bay during the summer of 1992; and a possible
second (or later)-generation hybrid of a female green turtle and male hawksbill
originally collected in Surinam in 1977 and maintained at Cayman Turtle Farm."^^
The existence of these hybrids is remarkable because the tribes Carettini and
Chelonini reflect an ancient division within the family Cheloniidae, dating to perhaps
50+ mya."^^ These crosses may be the oldest vertebrate lineages known to hybridize
in nature. Predictions about the impact of hybridization on natural populations are
difficult to make. It is therefore imperative that wildlife managers consider the
potential for hybridization before manipulating the natural histories of endangered
species.
38 The Biology of Sea Turtles
MATAPICA,
SURINAM
FIGURE 2.3 Green turtle nesting colonies at Matapica, Surinam and Ascension Island,
U.K. and distribution of tag recoveries along the coast of South America. Dark shading
indicates the area of overlap in feeding habitat along the coast of BraziL^^’-'’^The distribution
of mtDNA haplotypes (“C”, “D”, and “E”) are indicated for each rookery. Despite extensive
overlap on feeding grounds, no mtDNA haplotypes are shared between samples from these
two nesting colonies, consistent with a natal homing hypothesis for the migrations of nesting
females.^^’^^
isolation among nesting beaches. Five independent nDNA loci were screened for
RFLPs and the results were compared to the mtDNA analysis. Overall, there was a
low level of genetic variation observed in green turtles, consistent with previous
surveys using protein electrophoresis.“’^’^^ An analysis of nDNA interpopulation
genetic differentiation revealed that, although not as pronounced as the mtDNA data,
green turtle populations on a global scale are genetically isolated from each other.
The overall estimate of intraocean gene flow based on nuclear loci is higher than
that observed for mtDNA, but still indicative of restricted gene flow between nesting
colonies.
One of the primary goals of this study was to compare genetic results from the
strictly maternally inherited mtDNA with those of the biparentally inherited nDNA,
allowing the disentanglement of maternal from paternal influences on population
genetic structuring. The Ascension Island and Surinam nesting populations provide
an appropriate arena for such comparisons. The estimates of mtDNA (i.e., female)
gene flow between these populations was zero, because no mtDNA haplotype was
shared between populations. When assayed for genetic differences at the five nDNA
40 The Biology of Sea Turtles
loci, the two populations were indistinguishable from each other. Estimates of
nuclear gene flow between the two populations were quite high, indicating that there
is current, or recent historical, gene flow between these two populations. This nDNA
gene flow must be mediated by males. Comparisons of gene flow based on these
nDNA and mtDNA data sets must be made with caution due to the low level of
variation detected at assayed nDNA loci. Nonetheless, the conclusion of occasional
male-mediated gene flow between nesting colonies warrants further investigation.
FitzSimmons et al.^^ have developed a suite of highly polymorphic nDNA
markers that may be capable of resolving many of the remaining questions concern
ing male-mediated gene flow and population structure in marine turtles.
Mark/recapture studies have revealed the presence of cohorts from specific nesting
populations on feeding g r o u n d s , b u t the relative contribution of each regional
rookery to a feeding area remains unknown in most cases (but see Limpus et al.).^^
Satellite tracking has solved the problem of following individual turtles during
oceanic movements, and has revealed fascinating aspects of behavior and ecology.^®
However, the limited sample sizes currently available in satellite tracking studies do
not allow appraisals of population-level phenomena such as feeding ground com
position.
The unknown link between nesting populations and feeding aggregates
stands as one of the primary gaps in the scientific understanding of marine turtle
life history. Fortunately, the presence of rookery-specific genetic markers (and
42 The Biology of Sea Turtles
FIGURE 2.5 Distribution of loggerhead mtDNA haplotypes in Pacific nesting areas, North
Pacific driftnet fisheries, and Baja California feeding grounds?^ These data indicate that
juvenile loggerhead turtles from the nesting beaches in Japan traverse the North Pacific Ocean
in the course of developmental migrations. The westerly Northern Equatorial Current may
facilitate a return migration to adult habitats in the western Pacific. Juvenile turtles have been
recovered from New Zealand, Chile, and Colom bia,^invoking the possibility that some of
the turtles from the nesting colony in Queensland, Australia undertake a similar transoceanic
migration.
the scale of 7000 km which separates Bahia, Brazil and Mona Island, Puerto Rico.
Therefore, a harvest of hawksbill turtles on feeding grounds within the territorial
waters of one nation will affect nesting populations within the territorial boundaries
of other nations.
2.6 SUMMARY
In the near future, relatively complete genetic inventories of nesting populations for
each species are likely to be completed. These inventories represent a milestone in
the conservation genetics of marine turtles. The (foreseeable) advantages of range
wide surveys are threefold:
In addition to these benefits, molecular data are likely to illuminate other aspects
of natural history (such as paternity) which will have conservation applications
currently unforeseen by wildlife managers and biologists. In view of these ongoing
conservation benefits, it is regrettable that scientific permit restrictions continue to
play a role in defining the pace of conservation-oriented research.
The genetic data available to date prompt the following conclusions:
natal homing and reinforce the concept that marine turtle nesting colonies
constitute fundamental units of wildlife management.
The nDNA data indicate that significant levels of gene flow occur between
some regional nesting populations and that this gene flow is mediated
primarily by males. More sensitive nDNA (microsatellite) analyses are
cuiTently being applied to elucidate genetic structure over finer geographic
scales. Sequence data from nDNA loci has generally been concordant
with the phylogenetic inferences based on mtDNA sequences.
Rookery-specific mtDNA polymorphisms can be used to resolve the con
tribution of nesting populations to a particular feeding ground. Genetic
“tags” demonstrate that loggerhead turtles cross the Pacific Ocean in the
course of developmental migrations. Mixed stock assessments indicate
that loggerhead and hawksbill feeding grounds typically contain turtles
from several nesting populations. These data are concordant with tagging
studies in demonstrating the international nature of marine turtle conser
vation.
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drial DNA markers: origin of a confiscated green turtle. Mar. Turtle Newsl, 66, 1,
1994.
84. Swofford, D. L., PAUP: phylogeny analysis using parsimony, version 3.0.5, Illinois
Natural History Survey, Champaign, IL, 1991.
85. Limpus, C., personal communication, 1995.
Q Reproduction In Sea
Turtles
Jeffrey D. Miller
CO NTENTS
3.1 Introduction..................................................................................................... 52
3.2 Adults.............................................................................................................. 52
3.2.1 Distribution......................................................................................... 52
3.2.2 Size and Maturity................................................................................ 54
3.2.3 Reproductive Behavior....................................................................... 54
3.2.3.1 Philopatry.............................................................................55
3.2.3.2 Nest Site Fidelity.................................................................55
3.2.3.3 Periodicity........................................................................... 55
3.2.3.4 Courtship and Mating................................................... 57
3.2.3.5 Nesting Activity...................................................................58
3.2.3.6 Nesting Process....................................................................60
3.2.4 Reproductive Potential........................................................................ 63
3.2.4.1 Shape and Size of Eggs.......................................................64
3.2.4.2 Number of Eggs and Clutches............................................64
3.3 Embryos.......................................................................................................... 66
3.3.1 Fertility and Infertility........................................................................ 66
3.3.2 Development....................................................................................... 66
3.3.3 Nest Environment............................................................................... 67
3.4 Hatchlings....................................................................................................... 68
3.4.1 Hatching Process................................................................................. 68
3.4.2 Hatchling Emergence.......................................................................... 68
3.4.3 Hatching Success................................................................................ 69
3.5 Conservation................................................................................................... 69
3.5.1 Nesting Beaches.................................................................................. 69
3.5.2 Greenhouse Effect...............................................................................70
References................................................................................................................71
()-8493-8422-2/97/$0 00+$.50
© 1997 by CRC Press, Inc 51
52 The Biology of Sea Turtles
3,1 INTRODUCTION
Reproduction in marine turtles (Cheloniidae, Dermochelyidae) occurs within three
general constraints.^ First, nesting must occur during conditions which “are condu
cive to adult activity”.^ Second, nesting must occur during conditions which facilitate
embryonic development and survival. Third, hatchlings must emerge into conditions
that “are conducive to their survival”.^ Within these constraints, sea turtles share a
number of general reproductive characteristics. All species exhibit (1) iteroperous
reproduction^’^ with the possible exception of the Kemp’s ridley,^ (2) stereotyped
nesting behavior,"^ (3) laying of relatively large numbers of eggs several times during
the reproductive period,^’'’ and (4) relatively strong attachment to a particular location
for nesting,^’^ but inter- and intraspecific variations exists.^’^^ Together these con
straints and characteristics form the context of an ecologically successful reproduc
tive strategy.
Because most ecological studies on sea turtles have been conducted on the beach
during nesting, most deal with some aspect of reproduction. As a result the literature
concerning the reproductive biology of sea turtles is immense, albeit uneven. The
myriad research results concerning mating, nesting, behavior, reproduction, distri
bution, and population dynamics have been published in books,^'^"^ symposia,
r e v i e w j o u r n a l a rt i c l e s, s p e c ie s accounts,^^-^^ and collected references.^^’^^
In the current review, no attempt has been made to cite every paper that deals with
the topic; preferentially, reference has been made to the papers that provide clear,
concise information and that provide linkages into other relevant sources.
3.2 ADULTS
The seven species of sea turtles that reproduce on beaches around the world, Der~
m ochelys coriácea (leatherback turtle), C helonia m ydas (green turtle), Caretta
caretta (loggerhead turtle), E retm ochelys im hricata (hawksbill turtle), L epidochelys
olivácea (olive ridley), L. kem pi (Kemp’s ridley), and N a ta to r depressus (flatback
turtle), share a common life cycle with only minor variation^’^’^^ (Figure 3.1). All
species migrate, at least short distances, from foraging areas to mating areas, then
the males return to the foraging areas and the females move to the nesting areas.^^’^^
After a reproductive period of several months, the females return to their foraging
areas and begin to prepare for the next reproductive period, a few to several years
in the future.2^’“^
3.2.1 D istrìbution
As a consequence of their food and habitat requirements, adult sea turtles are
unevenly distributed throughout the circumglobal tropical and subtropical
While most species are widely distributed, L. kem pi is restricted
to the Gulf of Mexico'^^ and N. depressus is endemic to the Australian continental
shelf.Although foraging occurs over a wide range, including open ocean areas,
the primary foraging areas for marine turtles are located within the North and South
20°C isotherms of average sea surface temperature and on the relatively shallow
Reproduction In Sea Turtles 53
FIGURE 3.1 Generalized life cycle of sea turtles; individual species vary in the duration of phases. Dermochelys coriacea and at least some populations
of Lepidochelys olivacea remain palagic foragers throughout their lives. (Redrawn from Lanyon, J., Limpus, C. J., and Marsh, H., in Biology ofSeagrasses,
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54 The Biology of Sea Turtles
3.2.3.1 Philopatry
Carr^^ made the distinction between regional discrimination (philopatry) and fine-
scale homing to beach (site fidelity) within the region for sea turtles. It is now well
established that marine turtles migrate between their foraging areas and their nesting
areas with a high degree of accuracy.^’^^ Tag-return data have established that
although some turtles migrate greater than 2600 km, most travel less than 1000
The migrations of the green turtle across international borders are well
documented;^^’^^ in contrast, N. depressus does not migrate beyond the continental
shelf of Australia. All other species migrate to varying degrees.^ 56 ,57,61,63,68
Although they may not necessarily return to the beach of their birth, genetic studies
have demonstrated that breeding sea turtles return to the region of their birth.^^'^^
Once it has returned to the region of its birth and selected a nesting beach, a turtle
will tend to renest in relatively close proximity (0 to 5 km) during subsequent nesting
attempts within that nesting season, although a small percentage will utilize more
distant nesting sites in the general or within a few hundred kilome-
ters.88.89 Limpus et al.^^ reported that N. depressus returned to the nesting beach with
a high degree of accuracy regardless of whether they were returning after an “unsuc
cessful nesting attempt,... a successful nesting,... [or] in a later breeding season.”^^
Bjomdal et al.^^ reported the distance between intraseasonal renesting attempts of
38 loggerheads ranged up to 290 km; other records of intraseasonal nesting move
ments suggest that loggerhead turtles are capable of moving long distances, but the
proportion of individuals doing so is low.^' Green turtles show a high degree of nest
site fidelity. Bose and Le Gall^^ reported that most renesting attempts were within
200 m of the previous attempt, with a range of about 600 m over the 3-month nesting
season.
The ecological consequences of this behavior require elucidation, but it is clear
that the turtles are spreading their reproductive effort through time which reduces
the impact of an unpredictable environment on hatchling production.'^^ By laying
several clutches of eggs at approximately 2-week intervals, the likelihood of all eggs
being lost is greatly reduced.
3.2.3.3 Periodicity
In general, female sea turtles do not reproduce every
possible exception of L. kempi?'^^ However, males of at least some species (e.g.,
Ca. caretta) may breed every year"^'^’'^^’ or every two years. There is no reason to
assume that males and females use the same time scale for reproduction. The duration
of the period between reproductive seasons is defined as the remigration interval.^*
The mean remigration interval reported for female sea turtles varies among the
species (Table 3.1); the range in remigration interval is from 1 to 9 or more years,
depending on the s p e c i e s . 88,91 actual remigration interval for a pop
ulation may be longer than is currently understood; the discrepancy may result from
56 The Biology of Sea Turtles
TABLE 3.1
Reproductive Characteristics of Marine Turtles; Reproducing Females
Clutch
Dominant Carapace frequency Renesfmg Remigration
period of length (clutches/ interval (days) interval
Species nesting (cm) season) (mean, range) (years)
Dermochelys Night 148.7 (1.7) 11 6.17 (0.47) 4 9.5, 9-10 2.28 (0.14) 5
coriacea
Cheionia Night 99.1 (1.9) 22 2.93 (0.28) 9 12, 10-17 2.86 (0.23) 9
mydas
Natator Night and Day 90.7 (0.9) 6 2.84 (—) 1 16, 2-23 2.65 (—) 1
depressus
Lepidochelys Day 64.6 (—) Í 1.80 (—) 1 20-28* 1.50 ( - ) 1
kempi
Lepidochelys Night 66.0(1.1)8 2.21 (0.79) 2 17,22, 30,45* 1.70 (0.30)2
olivácea
Eretmochelys Night and Day 78.6 (1.7) 15 2.74 (0.22) 5 14.5, 11-28 2.90 (0.11) 5
imb ficaia
Caretta caretta Night 87.0 (1.6) 18 3.49 (0.20) 4 14, 13-17 2.59 (0.15) 5
Based on data from Hirth,^ Dodd,^^ and Van Buskirk and Crowder.“^
There are only a few, mostly anecdotal, descriptions of courtship behavior among sea
turtles in the literature. Ehrhart^®"^ tabulated 15 studies which provide the foundation
for the current understanding of courtship and mating (Ch. E.
imbricatap^ Ca. carettaP^^'^^^ L. kempip^^ the most detailed descriptions concern
green turtles.
The range and significance of precopulatory behaviors cannot be determined
without more detailed underwater studies. The range of species-specific signals that
denote receptiveness (possibly: head bobbing, position in the water column, head
to head bumps, nuzzling, biting, movement of flippers) remain undefined. Precop
ulatory behaviors may have greater significance to females than to males; during
the mating period male green turtles attempt to court and mount objects that are
approximately the right size and general shape (e.g., skindivers, rough wooden
decoys, etc.).^^"^ Mounted pairs are most often seen at the surface of the water, but
copulation may occur anywhere in the water column."^^’^^’^^’^®^
Certainly, courtship and mating are not gentle processes.A female receives
bites (nips) to her flippers, neck and head that leave open sores requiring several
weeks to heal. She receives damage to her shell from the claws of the males as well.
Throughout the subsequent nesting season, the progressive healing of these injuries
provides an indication that mating took place only during the prenesting season.
Males are not excluded from receiving damage while several males are courting a
female. As one male positions himself to mount or becomes mounted on the female,
attendant males often bite the trailing edges of his flippers and/or the dorsal portion
of his tail. This biting may or may not unseat the mounted male at the time, but
certainly reduces his fitness to attempt mating with another female by causing severe
damage to the flippers and the tail. The more severe the injuries, the greater the
chance that the mounted male will be removed from the breeding pool, at least for
a period.
Three studies that were conducted on captive animals^®’ provide details on
aspects that are difficult to obtain in wild situations (e.g., duration of coupling and
resulting fertilization rates, period of receptivity, period of courtship activity).
Although a pair may remain coupled for hours, the actual transfer of sperm may not
require the entire period.
The male mounts the female quickly, usually at the surface with a lot of splash
ing, and hooks onto her carapace using the enlarged claws on his front flippers and
the large claws on his hind flippers to hold himself in place. The male curls his long
tail to bring their cloacae into contact. His penis is erected into her cloaca. The shape
of the penis with a bifurcation of the sperm duct at the tip allows for the transfer of
semen into each oviduct without passing through the environment of the female’s
cloaca. In captive situations the pair may remain coupled for 10 or more hours;
58 The Biology of Sea Turtles
Wood and Wood^^ reported an increase in fertility that correlated with the duration
of the coupling period.
In the wild, after coming into reproductive readiness, females and males
migrate^’^^"^’^'^ from their foraging areas to copulate at courtship/mating areas^^ in
the region of the nesting area. Males show fidelity to particular courtship areas in
successive migrations.Females are in reproductive readiness for about 7 to 10 d;
males appear to be sexually active for about one month.^^’^^'^'’ Genetic studies have
demonstrated that males mate with several females and females mate with several
m a l e s . A t the end of the mating period the males leave, presumably returning
to their foraging areas.^^ Female green turtles^^ (and presumably females of most
other species) disperse from the mating areas to nesting sites in the region to which
they show fidelity.
Some confusion concerning courtship and malting crept into the literature
because some of the early observations suggested that mating for fertilization of
subsequent clutches occurred immediately following oviposition and that females
retained sperm for several years between reproductive Even
tually these ideas were challenged by Frazier,"^' who raised the following points: (1)
lack of evidence that females without claw marks on their carapace (i.e., not mated)
laid fertile eggs; (2) the nonadaptive requirement that virgin females migrate to mate,
but not lay eggs; (3) the nonadaptive behavior required of males to migrate before
mating; and (4) the mechanism to ensure sperm viability following long-term stor
age."^^ It is now generally accepted that mating occurs in the month or two just
preceding the first ovipositional cycle of the season.^^ Sperm have been found at the
junction of the magnum and the aglandular zones in the upper reaches of the
oviduct; presumably, the sperm in this area fertilize the ova as they enter the
magnum, which secrets albumen. Females do not store sperm between reproductive
seasons.
Absolutely nothing is known about the impact of endogenous factors, such as
ageing, on reproduction. For example, older males may have a longer period of
spermatogenesis than younger males. Similarly, older females may nest earlier in
the season and may lay more clutches. The testing of these ideas requires a known
age population. As individuals in long-term studies pass through puberty, join the
breeding portion of the population, and age, it may be possible to address these
issues.
Although there have been several attempts to define the qualities of a nesting beach,
it is not obvious why a population of sea turtles or an individual sea turtle uses a
particular beach.Mortimer^^^ identified general requirements for nesting beaches:
(1) the beach must be accessible from the sea, (2) the beach must be high enough
to prevent inundation of the eggs by tides or the underlying water table, (3) the
substrate must facilitate gas difussion, and (4) the substrate must be moist and fine
enough to prevent collapse of the egg chamber during construction. Mortimer^^®
examined physical and chemical characteristics of beach sand from 50 nesting areas
from around the world and found that variability was high. She concluded that
Reproduction In Sea Turtles 59
results from the habitat in which the turtles typically nest and some of the variation
is derived from the size of the turtle (among the cheloniid turtles, smaller species
nest faster).
TABLE 3.2
Stages of Sea Turtle Nesting Behavior
Activity Species Differences
Stranding, Testing of sand, and Similar in all species; D. coriácea may be more direct than other
emergence from wave wash species; L. olivácea and L. kempi in arribadas
Ascent of the beach Similar in all species; usually fairly direct uphill unless debris causes
deviation
Selecting nest site Similar in all species; may be related to change in temperature
Clearing nest site All species actively clear area, possibly in response to litter and
vegetation at site
Excavating body pit Similar in all species; Ch. mydas digs proportionally deep body pit
Excavating nest hole Similar in all species; achieved by alternating use of hind flippers
Oviposition Similar in all species; hind flipper positioned differently
Filling and packing nest E. imbricata ladles sand over eggs; others scrape sand into hole; N.
chamber depressus, L. olivácea, m á L . kempi rock body forcefully to compact
sand; D. coriácea pivots with weight on rear body; other species use
hind legs to “knead” sand compact
Filling body pit Similar in all species; sand thrown backwards by front flippers
Course back to sea Similar in all species; move down slope
Reentering wave wash Similar in all species; typically a pause at edge of waves
process involve about 50 separate action patterns. These action patterns are thought
to be modified locomotor patterns derived from the gait. This level of analysis should
be applied to the other species to define similarities and differences among them.
Although the physical characteristics (slope, aspect, size of particles) of the
beach influence the nesting behavior, as well as the microenvironment in which the
eggs develop, in general the nesting sites used by sea turtles share several charac
teristics: exposed marine beaches, relatively loose sand, free of buried debris, and
above high tide level.
At the moment when the turtle is first supported by the sand it may be washed
over by several waves before it starts up the beach; while the turtle is on the wet,
hard, sloping part of the beach she continues mostly uphill. As the turtle proceeds,
it pauses after a few meters to rest, breathe, and, possibly, observe its surroundings.^^
During this period solitary and low-density nesting marine turtles are easily deterred
from continuing up the beach by activity on the beach; during arribada nesting by
L. olivácea and other high-density nesting situations (e.g., several thousand green
turtles attempting to nest at Raine Island, Australia; personal observation) turtles are
less easily disturbed. Debris and other obstacles may cause the female to change
direction or even to abandon the effort; she may return to the water for no obvious
reason. The tracks of aborted nesting attempts are often and erroneously called “false
crawls”;’“"^ the use of this term may be acceptable in the vernacular, but it is
misleading and implies some intent on the part of the turtle. The crawl is real; the
nesting attempt was aborted for some reason, albeit unknown. The occurrence of
aborted nesting attempts varies among species (low in N. depressus,^^ high in Ca.
caretta^^ and between nesting beaches (e.g., high in southeastern U.S., low in
Queensland, Australia).W hatever the reason(s) for aborting the nesting attempt,
the turtle usually returns to nest the same night or the following night (e.g., Ca.
caretta, 1.08 d;"^^ N. depressus, 1.17 d)^^ and most return to the same beach (e.g.,
Ca. caretta, N. depressus, 95%).^^
Once the turtle has crossed the temperature boundary between the cooler wet
sand of the beach slope and entered the warmer, drier, softer, flatter portion of the
b e a c h s h e may change direction several times before selecting a site at which to
construct her nest.^^^ All sea turtles prepare the nest site before digging the egg
chamber to varying degrees. The surface debris is removed by either simultaneous
or alternating sweeps of the front flippers. As the area in front of the turtle is cleared
the turtle “swims” forward onto the cleared substrate. The hind flippers may be used
to clear loose, dry substrate from immediately behind the turtle. The depth of the
body pit is related to the dryness of the surface sand and to the size of the turtle.
Very dry conditions require that a turtle digs deeper to locate the level of firmer
sand that will hold the shape of the egg chamber. Larger species (e.g., green turtles)
excavate relatively deep body pits; whereas smaller species (e.g., hawksbill turtles)
dig shallower body pits. After the turtle has moved forward into the “body pit”, the
front flippers cease action and are usually positioned near the carapace.
Sea turtles use their hind flippers to excavate a flask-shaped chamber that has a
narrow neck and a wider bottom"^^ unless the dryness and particle size of the substrate
causes the shape to a l t e r . A t first, the nesting sea turtle scrapes and flicks sand
62 The Biology of Sea Turtles
away from the area where the chamber will be; then it begins to scoop sand from
under its posterior using the ventral portion of each hind flipper altematively.^^’^^^
The cup full of sand is placed to the side of the chamber area and the flipper is
quickly placed on top of it.^^ The other flipper is then used to remove a cup full of
sand; when this flipper is placed to the side of the chamber, the first flipper throws
the loose sand that has fallen on top of it forward and reaches into the nest chamber
for more sand.^^ This process ensures that during the filling-in process, the sand
placed on top of the eggs is moist sand that originated from the chamber as it was dug.
The actual shape of the chamber is constrained primarily by two anatomical
factors: the length of the hind limb and the size of the flipper (hand) portion. Because
hawksbill turtles have relatively short hind limbs and relatively small hind flipper
areas, they dig a comparatively shallow nest chamber that has a more rounded bottom
portion.^^ In contrast, green turtles have relatively long hind limbs with relatively
large hind flippers; as a result, the egg chamber dug by a green turtle is deeper and
extends more forward than that dug by a hawksbill turtle. Regardless of the size of
the hind flippers, by raising on its front flippers, a turtle is able to extend its reach
down and forward in the nest chamber.^^ The shape of the chamber is also effected
by the moisture content and particle size of the substrate as well as the presence of
buried debris. The nest is not inspected visually during the entire process; the
stereotyped alternating digging stops when the turtle cannot remove any more sand
while attempting to scrape the walls and bottom of the chamber. The digging
behavior is stereotyped so strongly that a turtle missing one hind flipper will move
the stump in sequence with the flipper actually removing sand from the chamber.
This one-legged style concludes with a lopsided chamber, the walls of which cannot
be enlarged by the one flipper, but that is not large enough to hold the entire clutch
of eggs. In these cases the eggs fill the neck of the chamber and may spill out the
top where they are mixed with sand during the filling process.
During oviposition the hind flippers of Dermochelys, Chelonia, and Natator are
positioned to cover the nest chamber; one flipper usually hangs inside the chamber.
In this position, one flipper may help keep sand from falling into the chamber while
the other may break the fall of the eggs into the chamber. In Carena, Eretmochelys,
and Lepidochelys both hind flippers are extended outwards on the sand behind the
turtle so that the opening to the nest is not covered by the flippers, but is covered
by the posterior margin of the carapace;^^ during oviposition the outer edges of the
flippers curl as eggs are released.
The eggs are laid singly or in groups of two or three, sometimes four. As the
cloaca contracts, the tip of the tail points downward and slightly forward, then relaxes
backward as the eggs drop. Results of a dissection of a green turtle that died during
oviposition at Raine Island, Australia indicate that eggs were laid from both oviducts
together, rather than from one and then the other, because 22 and 27 eggs remained
in the oviducts while 47 eggs were in the nest chamber at the time of death (Miller,
personal observation).
During egg laying, all species of sea turtles are relatively tolerant of a modest
level of external disturbance. The level of indifference to disturbance varies among
individuals; some will be completely disturbed by being tagged while others will
Reproduction In Sea Turtles 63
bite the sand in front of them; still others will show no response at all. Part of this
variation is a function of the number of eggs already laid. As a general rule, the
level of tolerance increases as the turtle lays more eggs. The level of tolerance to
disturbance remains high during the filling of the egg chamber and the covering
phases of the nesting cycle.
Once oviposition is complete the nest chamber is filled in, usually by scraping
moist sand from near the opening into the hole with the hind flippers, until the sand
above the chamber is higher than the floor of the body pit.^^^ As the neck of the
chamber fills, the sand is compacted using the hand and leg portions of the hind
limb. In the smaller species the compaction may appear quite vigorous.
After the hole is filled and compacted, the front flippers begin to throw sand
backwards over the nest site; during the process the turtle moves forward and
continues to throw sand backwards. At the end of the process the nest site may be
one or more meters behind the turtle and the remnant of the body pit is not near the
eggs.129 behavior was once interpreted to be camouflaging the nest,^'" presumably
from predators; while this interpretation may be in part true in some cases, most
predators can readily locate a nest within a few days of oviposition or near emer
gence. The primary function of this behavior is to reestablish the environment of
the beach around the nest; the sand thrown on top of the nest provides insulation in
terms of both temperature and moisture for the eggs.
follow ing a divSturbance, and not a new clutch.^^ Because sea turtles may be deterred
from nesting by a variety o f subtle events, Limpus'^^ defined an internesting interval
as the interval between returning to the sea after having laid a clutch o f eggs and
when the turtle next emerges to lay, whether or not that attempt is successful.
All marine turtles lay white, spherical cleidoic eggs with flexible calcareous shells.^^
The size (diameter, shape) of eggs laid in each clutch varies slightly between clutches
laid by one female as well as considerably within and between species.^ D. coriácea
lays the largest eggs of all marine turtles (5.3 cm, 90 g), but N. depressus produces
eggs that are nearly the same size (5.1 cm, 80 g). Ch. mydas lays medium large eggs
(4.5 cm, 48 g), followed by Ca. caretta eggs (4.0 cm, 36 g); E. imbricata lays small
eggs (3.8 cm, 28 g), L. olivácea and L. kempi lay eggs of similar size (3.9 cm, 35
g; 3.8 cm, 30 g, respectively) (Table 3.2). At the level of species, large sea turtles
lay large eggs and small ones produce small eggs,^
In an examination of correlations among several life history traits at the species
level. Van Buskirk and Crowder^ used the means obtained for populations as reported
in the literature; they acknowledged that the data were uneven and contained errors.
Their analysis supports several generalities concerning sea turtle biology. They
reported that body size is correlated with “several reproductive traits including egg
size and overall reproductive effort”.'’ A general “trade-off between clutch size and
egg size was confirmed among the species” after adjusting for body size.^ However,
in their discussion of the unique pattern of a small clutch (mean = 52.8 g) and large
egg size (51.5 mm) found in N. depressus, they did not consider that Natator evolved
in the context of a wide range of carnivorous marsupials and varanid lizards that
occupied predatory niches in Australia similar to those occupied by placental car
nivores elsewhere.^^’*'*^ As would be expected, some traits (e.g., egg survivorship,
remigration interval) were not related to the body size of the female.^’Although their
analysis provides a number of insights into life history variation among the species
of marine turtles, until within and between regional variation and interpopulation
variation in life history traits for each species are better documented, including both
morphometric and genetic data, their analysis at the species level may be premature.
One factor that has not been adequately handled in assessment of fecundity in
sea turtles is the duration of the reproductive life of the species. Fecundity should
be calculated as the product of the clutch count, the number of clutches per breeding
season, and the number of breeding seasons in the life of the turtlehowever, the
number of breeding seasons in the life of a sea turtle is not known for any species.
Until reproductive life expectancy is determined, detailed comparisons of fecundity
among species is not Justified.^^
The number and size of eggs, and the number of clutches laid (Table 3.3) represent
the result of an adaptive compromise for survival.^^ The number of eggs in a clutch
should be the result of the interaction among optimum egg size for hatchling/post-
Reproduction In Sea Turtles 65
TABLE 3.3
Reproductive Characteristics of Marine Turtles: Nesting Period and Sizes of
Eggs and Hatchlings
Hatchling
Clutch count Egg weight Egg diameter Egg volume weight
Species {# of eggs) (g) (mm) (cc) (g)
Derm ochelys 81.5 (3.6) 12 75.9 (4.2) 4 53.4 (0.5) 9 79.7 (2.4) 9 44.4 (4.16) 5
coriacea
Cheionia 112.8 (3.7) 24 46.1 (1.6) 10 44.9 (0.7) 17 45.8 (1.2) 17 24.6 (0.91) 11
mydas
N atator 52.8 (0.9) 6 51.4 (0.4) 3 51.5 (0.3) 6 70.8 (1.1) 6 39.3 (2.42)3
depressus
Lepidochelys 110.0 (—) 1 30.0 (—) 1 38.9 (—) 1 30.8 (—) 1 17.3 (—) 1
kempi
Lepidochelys 109.9 (1.8) 11 35.7 (—) 1 39.3 (0.4) 6 31.8 (1.1) 6 17 ( - ) 1
olivacea
Eretmochelys 130.0 (6.8) 17 26.6 (0.9) 5 37.8 (0.5) 1 28.7 (1.3) 11 14.8 (0.61)5
imhricata
Caretta 112.4 (2.2) 19 32.7 (2.8) 7 40.9 (0.4) 14 36.2 (1.1) 14 19.9 (0.68) 7
caretta
Values are means of means of populations (standard deviation), number of populations included.
hatchling survival and dispersal, the selective advantage of the number of hatchlings
produced, and the physical and ecological limitations of the female. The number of
clutches laid during a reproductive period is the result of selection for the advantage
of separating groups of eggs in time and space in the context of the capacity of the
female to produce the eggs within the risks such as exposure to predation operating
within other limitations such as the length of the nesting season and the energy
required for reproduction.^^ Moll^^^ proposed two general patterns of reproduction
in turtles. The sea turtles fit pattern I: ‘Targe clutches of relatively small eggs; multiple
clutches produced during a ... nesting season; communal nesting in ... ancestral
nesting areas; careful construction of covered nest”.^° Within this paradigm, selection
favors fecundity; however, when approaching the extreme case, density dependent
factors such as predation (including by man) may intensify to cause local and
sometimes large-scale extinctions. The density of turtles attempting to nest on a
beach may have a direct impact on the number of eggs destroyed by subsequent
nesting attempts. Bustard and Tognetti^"^® reported on computer simulations of the
impact of various densities of nesting green turtles on egg destruction; they reported
that as density increases, so does the likelihood of clutches being destroyed. On the
nesting beach, density should be interpreted as both the number of turtles attempting
to nest and the number of clutches already deposited into the beach. However, the
importance of egg destruction should be reconsidered in the context of other factors
(e.g., bacterial infection, losses caused by inundation and erosion, etc.) that impact
66 The Biology of Sea Turtles
3.3 EMBRYOS
In order to provide for the next generation, adult sea turtles must lay fertile eggs at
the right stage of development into an environment that facilitates development and
maximises hatchling emergence.^
3.3.2 D evelopment
When a sea turtle arrives at the nesting beach she is carrying a full complement of
follicles to supply yolks for all the eggs she will lay during the season; the diameter
of the follicles will increase only slightly as the result of the absorption of water in
the period before ovulation.
Within the body of the female, a short period after the follicle has been ovulated
from the ovary, it enters the infundibulum of the oviduct; the follicle then passes
through the aglandular region into the magnum, where it is fertilized by sperm.
Because the sperm from all successful males are stored and mixed in the upper
portion of the oviduct, the follicles ovulated to become the yolks of the eggs in a
clutch are fertilized by sperm from several different males.Development begins
immediately following fertilization. The fertilized embryonic area on its yolk con
tinues into the oviduct where albumen is secreted from special cells in the lining of
the anterior glandular region of the oviduct. Once the yolk has been coated with
albumen, the inner shell membrane begins to foim, secreted from special cells in
the shell-forming segment of the oviduct.^^’^*'^’^"^^Following the formation of the shell
membrane, aragonite crystals begin to form the outer portion of the s h e l l . T h e
shell is not fully formed until at least the seventh day following ovulation.^'^
Although cleavage begins within hours of fertilization, development does not
advance beyond middle gastrulation while the egg is within the oviduct.^^ Eggs are
laid at middle gastrulation, before the chordomesodermal canal breaks through into
the subgerminai cavity. However, once oviposited, development resumes in a few
hours (4 to 8, depending on temperature); movement of the eggs after this time
results in death.
The first external sign that development is progressing is the development of a
white spot on the uppermost part of the egg;^^’^"^^ the embryo is situated just beneath
the shell. As the white spot becomes progressively bigger to eventually encompass
the entire shell, the embryonic membranes (vitelline, amnion, allantois, chorion)
Reproduction In Sea Turtles 67
that the hydric conditions will have a significant impact on the hatching success as
well as the mass and general vigor of the hatchlings.
The developing embryo usually experiences adequate gas exchange,‘22,162
although the gas diffusion may be affected by the particle size and water content of
the sand.‘22^162-166 inadequate gas exchange slows growth and increases embryonic
mortality.
3.4 HATCHLINGS
The eggs of marine turtles hatch after 6 to 13 weeks of incubation, depending on
the temperature.29 Hatchling sea turtles usually weigh slightly less than 50% (41 to
62%2) of their ovipositional egg weight (Table 3.2); large eggs produce large
hatchlings.^
because the energy input from the sun is removed and the gradient of latent heat
contained in the sand reverses. After an hour or more the temperature of the sand
at the level of the hatchlings has dropped and the hatchlings begin to dig toward the
surface, arriving after dark.
The gas exchange at the level of the hatchlings may also act as a controlling
factor on digging activity. The digging activity of the massed hatchlings may require
more oxygen (or produce more carbon dioxide) than can readily diffuse through the
sand. In this case the hatchlings may become quiescent until their anaerobic respir
ation debt is repaid. The behavior of hatchlings near the surface may not be regulated
by oxygen as much as by temperature. Clutches of hatchlings emerge through the
final few centimeters of sand on cool, cloudy, and/or rainy days.^^®
Hatchlings face two problems if they emerge during the day: lethal temperatures
and predators. The temperature of the sand during the day may exceed 45°C at some
nesting beaches; certainly if the hatchlings have to cross a wide beach under direct
sun, many will die before reaching the water or receive burns from the heat of the
sand being crossed and die from the burns later or be unable to swim properly and
be consumed before they reach deep water. Further, the hatchlings are much more
visible to a wide range of predators during the day. Day or night and at different
tide levels, hatchlings face a large number of aquatic predators that reduce the
number of hatchlings reaching the relative safety of offshore water.
3.5 CONSERVATION
The general policy for the conservation of marine turtles was outlined in Bjorndal;^
variations and refinements of these ideas have been presented by Limpus.^^^ Recently
the lUCN Marine Turtle Specialist Group produced a global strategy that addresses
research, management, and conservation issues. These policy statements provide a
framework for global, regional, and local conservation efforts. BjorndaF also out
lined what was (and is) being done so that others may copy successful conservation
programs; together, these documents outline the data required to better define the
biological requirements of the turtles.
obvious that the important nesting areas, nesting activities, as well as the eggs and
hatchlings should be protected.^ However, achieving this may take different forms
depending on the assessment of the local problem(s); in one area minimizing
lighting^’ near an important nesting beach to reduce disorientation will solve a
specific problem, while in another area, limitation of human access to an important
nesting area may alleviate several problems.^’
Management of the nesting site must include management of embryos via their
eggs. Probably the best conservation action on the nesting beach is to leave the eggs
in situ and to reduce the impact of predators and poachers. However, in certain
circumstances, the shifting of eggs to protect them from inundation, predators,
poachers, etc., is more effective. If practical, eggs should be shifted to nearby sites
out of harm’s way in preference to shifting them to hatcheries. Whenever it is
considered necessary to shift eggs to safer areas of the beach or to hatcheries, they
should be moved and reburied within 4 h of being laid or allowed to remain in situ
for at least 25 d to reduce the impact of movement-induced m o r t a l i t y . T h e new
location for the eggs must provide adequate moisture, temperature, and gas exchange
to support the developing embryos. It is almost self-evident that hatchlings should
be released as soon as possible after emerging from the sand.
The second area of focus is habitat protection. This may be achieved through
the creation of special, limited-access areas such as national parks or other protected
areas. Protection of the nesting beaches is an obvious priority; foraging areas, too,
should receive high priority because it is from the foraging areas that the energy for
yolk deposition (and hence embryonic, hatchling, and post-hatchling survival) and
migration is obtained. However, other habitats used by the turtles also require
protection, including mating areas, internesting areas, migration routes, and hiber-
nacula.^ Practical measures for the conservation of sea turtles are discussed by
Pritchard et al.;^^"^ whatever techniques are employed, they must be based on a
knowledge of the needs of the turtles and an assessment of the problem. Further,
whatever remedial action is applied must be documented so that the success or failure
of the effort can be evaluated. Because sea turtles are long-lived animals, the results
of conservation management actions will not necessarily be obvious, particularly in
the short term.
temperatures with higher values recorded at nesting sites closer to the equator. Along
with sea level rise there will be increased stomiiness (frequency and intensity) which
will reduce the size of islands, particularly low sandy ones, and alter the shape of
mainland beaches.Questions arise concerning the behavioral response of the
turtles: will they shift nesting sites? Genetic studies suggest that they will not en
mass, but that some will. Will the turtles simply continue to nest until the recruitment
fails to support the population? With increased storminess, will the associated cool
ing pulses of temperature at egg depth compensate for the general temperature rise
and thereby help to maintain the required sex ratio? It is predicted that ocean currents
may alter; if this happens to a current that is supporting the dispersal of hatchlings
from a nesting site, will the hatchling survivorship be increased or decreased or
unchanged? Given the known impact of the ENSO on the number of green turtles
nesting*^^ and that the impact of the greenhouse changes (more toward the anti-
ENSO), will green turtles nest less frequently? In the face of complex responses to
long-term changes, more data are essential before management actions are imple
mented. The short-term impact of low-temperature pulses associated with humcanes
and cyclones needs to be examined with reference to the experimental work on sex
determination.^^® The behavioral response of nesting turtles to alterations in the beach
and off-shore environment resulting from tropical storms should be monitored to
give a basis for prediction of the changes that might result from greenhouse changes.
A great deal is known about the reproductive biology of marine turtles, but there
is much more research to be conducted if man is to reconcile his behavior with the
nonnegotiable biological constraints of marine turtles. Their need for unpolluted
foraging areas from which to gather the energy necessary for growth and reproduc
tion must be respected. Their need for unencumbered nesting areas that they have
selected, on which they may lay their eggs, must be guaranteed.
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80 The Biology of Sea Turtles
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4 The Nest Environment
and the Embryonic
Development of Sea
Turtles
Ralph A. Ackerman
CO NTENTS
4.1 Introduction..................................................................................................... 83
4.2 Embryonic Development................................................................................ 84
4.3 Temperature..................................................................................................... 85
4.4 Water......................................................................................................... 89
4.5 Gas Exchange................................................................................................. 97
4.6 Altered Beaches.............................................................................................. 99
Acknowledgments..................................................................................................103
References..............................................................................................................103
4.1 INTRODUCTION
Sea turtle eggs are deposited in a large clutch in a nest excavated by the female in
sandy marine beaches. Incubation requires several months during which time the
sea turtle embryo grows from a few cells to a fully formed organism capable of
independent existence. During this process energy stored in the egg by the female
is transformed into embryonic tissue. The developing embryo is coupled to the
nesting beach through the exchange of the O2 , CO2 , H2 O, and heat that is either
required or produced by the energy transformation process. The nesting beach is
the incubator for sea turtle embryonic development and operates by producing a
climate space that is appropriate for embryonic development. Deposition of the egg
clutch depends on the ability of the female to select and excavate a suitable nest
chamber. Successful incubation of the eggs depends on the presence of suitable
conditions in the beach sand. Among these conditions are temperature, humidity or
water potential, salinity, and levels of respiratory gases. A microclimate suitable for
incubation is generated by interaction among the physical characteristics of the
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© 1997 by CRC Press, Inc 83
84 The Biology of Sea Turtles
material composing the beach, the physical structure of the beach, local climate,
and the eggs in the clutch. The microclimate is dynamic and changes with the state
of biological activity in the clutch and in the beach. Unfortunately, we know very
little about the microclimate of sea turtle eggs during incubation. We know even
less about how the physical characteristics of the beach influence the microclimate.
Our understanding of sea turtle embryonic adaptations for development in an
egg clutch buried in a nesting beach is not extensive. There is little information
available to describe how variation in beach characteristics, hydrology, topography,
or local climates influence the environment around eggs. The coupling between eggs
and beach is poorly described. No detailed climate space for a reptile egg of any
species has been described and, as a result, egg, embryonic, and developmental
adaptation are not well understood. This extensive lack of infomiation and insight
will not be remedied in this chapter. My focus will be on the environment for
incubation and on conceptual ways of examining and describing it while pointing
out remaining issues. Unfortunately, the lack of hard information on sea turtle
embryonic development and its environment limits the extent to which conceptual
synthesis is possible.
used for differentiation and growth. Ackerman^ measured the O2 uptake of Caretta
and Chelonia eggs during development. The pattern of change in O2 uptake with
time was sigmoidal, peaking at about the time that hatching occurred and could be
described with a logistic curve. Vleck and Hoyt^ reported data for Dermochelys and
summarize the available data for reptile eggs in general. Peak O2 uptakes are a
function of initial egg size and, presumably, hatchling mass and increase with about
the 0.8 power of initial egg mass. Peak O2 uptakes for sea turtles are in the range
of 90 to 250 ml O2 day'k Total O2 cost of incubation is in the range of 38 to 90
kJ (after converting to energy units) and increases with about the 0.8 power of initial
egg mass. Note that the O2 cost is an estimate of the energy lost due to metabolic
inefficiency during development and is not an estimate of the total cost.
4.3 TEMPERATURE
Reptile embryos develop successfully over a range of temperatures.^'^ Typically,
incubation period (I, days) decreases as incubation temperatures (T, °C) increases.
The reciprocal of I can be used as a developmental rate (R^, days“^-^ Ackemian^
discusses how the relationship between I and T can be described. One descriptor is
the range of T over which development occurs with the end points being described
as upper and lower lethal temperatures. Another is the position of the curve relating
I or R^ to T (the response curve) on the T scale. A third is the slope of the response
curve of I or R^ to change in T. Sea turtle eggs are typically incubated at constant
temperature in the laboratory where I can be determined with the greatest precision.
Eggs in nature do not experience constant temperatures. While diurnal temperature
changes in the beach are damped by the depth of the nest, there is still likely to be
some diurnal change, and furthermore, nest temperatures may change systematically
over the course of incubation (see discussion below). Because there are no experi
mental or empirical observations, it is not clear whether or not the average of a
changing temperature produces the same effects as a constant temperature of the
same value. However, because temperature effects are typically nonlinear,^ we might
expect differences.
The thermal tolerance range (TTR) for development of sea turtle embryos
incubated at constant temperature appears to fall between about 25 to 27°C and 33
to 35°C and is around 10°C wide. Data for I and T for two sea turtle species are
shown in Figure 4.1. The data appear nearly linear as do the summarized data of
Miller. ‘ However, temperature effects on growth rate are typically nonlinear,^ and
nonlinear curves yield better fits to the data than do linear ones. The Arrhenius
equation is often used to describe temperature influences on rate functions^ and
takes the form:
R.
where R^ is a rate (days the pre-exponential factor (B, days'O. represents the
position of the curve on the T scale, R (kJ • mok^ • K~^) is the gas constant, T (°K)
is the incubator temperature, and E (kJ • mol~0 is the free energy. The quantity E
86 The Biology of Sea Turtles
FIGURE 4.1 The influence of incubation temperature on incubation time. Open circles are
for the hawksbill turtle, Eretmochelys imhricata. The closed circles are for the loggerhead
turtle, Caretta caretta. Data are taken from the literature cited in the text.
can be used to describe the shape or sensitivity of the response of (or I) to change
in T. Q,o can also be used to define the shape or sensitivity,
is not well defined. Figure 4.2 illustrates the influence of constant incubation tem
perature on hatchling sex ratio for 4 species of sea turtle. The data are taken from
Paukstis and Janzen’^ and Mrosovsky et al.^^ A cumulative normal distribution
(represented by the line) is fitted to the data. This distribution is often used to describe
dose-response relationships.The estimated Tp^^ is 29.0°C with a standard deviation
of 1.77°C. Table 4.1 summarizes Tpj^ estimated by linear regression on data for the
individual species as well as on the pooled data. There is considerable scatter in the
data both within and among the species, but the Tp^^ values are quite similar among
the species. The data sets are not of sufficient quality to determine whether or not
Tpj^ varies within species. Typically, because of the endangered and threatened status
of sea turtles, only small samples with few replicates are collected. If we treat sex
determination as a binomially distributed random variable (i.e., either male or
female). Figure 4.3 illustrates the random variation which we might expect from
samples of size 10, replicated 10 ten times. Here, 10 replicates were drawn for 5
different, evenly spaced probabilities ranging from 0.1 to 0.9 with Tpi^ (p = 0.5) set
to 29°C. The situation is very much improved for larger sample sizes on the order
of 50 eggs. Much of the variation that is seen in sea turtle TSD data is likely to be
random. Tpj^ is of interest because it ought to be useful for predicting the beach
temperature required to produce a sex ratio of 50%. This assumes that the Tpj^
produced by constant incubator conditions can be extrapolated to the beach where
temperature is not constant. Mrosovsky^ reports that beach temperatures in some
locations are around the levels of Tp^^, but in other locations, such as the Atlantic
coast of Florida, average beach temperatures appear to be in excess of Tpj^ during
incubation with an excess production of female hatchlings as a result. The life history
consequences of imbalances in sex ratios are poorly understood.^ One problem, as
pointed out by Mrosovsky,^ is that we know little about the long-term variation of
beach temperature. This is of particular importance for long-lived animals such as
sea turtles. Unfortunately, there are few long-term temperature data available for
beaches. I have found a 12-year record for soil temperature at 10 cm near Gainesville,
FL. Figure 4.4 illustrates average July soil temperatures. The data for June and
August are similar. Although temperatures in beaches at greater depth are likely to
be somewhat cooler, we can see that the average temperature near Gainesville is
typically well in excess of Tpj^. This is consistent with the trend for nesting beach
temperature noted by Mrosovsky^ from a much smaller data base: beach tempera
tures at appropriate depths seem to be in excess of Tpj^ and ought to produce more
female than male hatchlings. It is possible if not likely that the Florida beaches have
been warm (in excess of Tpj^) for some time, perhaps in excess of a decade. To
understand TSD in this system we need to know what the normal beach temperature
is and how it varies, at least over some period of time in excess of the life span of
the sea turtles nesting on the beaches. This is a problem, but could potentially be
approached by correlating beach temperature with the temperature at some known
(and, hopefully, close) location.
Because temperature has such a pervasive influence on the embryonic develop
ment of sea turtles, it is important to understand the determinants of clutch temper
ature. The most important determinant is, of course, beach temperature. The second
important determinant is the exchange of heat between the clutch and the beach.
88 The Biology of Sea Turtles
FIGURE 4.2 The influence of constant incubation temperature on hatchling sex ratio of
sea turtles. Loggerhead turtles (open circle), ridley turtles (diamond), green turtles (plus), and
hawksbill turtles (square) are shown. Data are taken from the literature as described in the
text. The line represents a cumulative noiTnal distribution fitted to the data as described in
the text.
TABLE 4.1
Pivotal Temperatures (°C) of Several Sea Turtle Species
Pivotal
Species temperature 95% Cl R2
FIGURE 4.3 The number of females resulting from samples from a binomial distribution.
Clutch size was 10 eggs and each sample for 5 probabilities between p = 0.1 and p = 0.9 was
run ten times. Only different results are shown. The line is a linear regression through the
data. At p = 0.5, Tpj^ was 29.0°C.
4.4 WATER
Sea turtle eggs contain a supply of water when they are oviposited. Water is stored
in the albumen at the beginning of incubation and is supplemented by water gained
through the transformation of yolk.^^ Because there are a number of anatomically
and physiologically different compartments inside the eggs, including the embryo
and extraembryonic membranes, the yolk sac, the chorioallantoic sac, and the
90 The Biology of Sea Turtles
FIGURE 4.4 The average daily temperature at a soil depth of 10 cm in July at Gainesville,
FL. The solid line represents pivotal temperature for sea turtles. The asterisks represent average
daily temperatures for July 1995,
amniotic sac, water and osmotic balance may be complex. Vleck^^ has reviewed the
water economy and osmotic regulation of a number of reptile species, but we know
very little above the osmotic events and processes occurring inside the sea turtle
egg at any stage of embryonic development. Egg and embryonic osmotic balance
are especially interesting because the egg must be open to the exchange of water
vapor with the environment in order to exchange respiratory gases. The sea turtle
egg is coupled to the environment through the exchange of water vapor and possibly
through the exchange of liquid water.^^'^^ We know that for some species of reptile,
embryonic growth is influenced by egg water exchange^“^’^^ while for other species,
embryonic growth is independent of egg water exchange over some range of
exchange rates. Some reptile eggs must take up water in order to produce a viable
hatchling.A ckerm an et al.^^ suggested that water exchange was likely to be
relatively less important for large eggs such as sea turtles than for smaller reptile
eggs. We have recently found^^ that the embryonic growth of Caretta caretta is
independent of egg water exchange over a range of total egg water exchanges of
around -10 to +30% of initial egg mass. There also appeared to be little effect on
hatchability over this range, which is consistent with the observations of Bustard.*^
It is clear that hatching success can be correlated with some physical character
istics of the sand environment.-^^ Mortimer^^ found that hatching success of Ascension
The Nest Environment and the Embryonic Development of Sea Turtles 91
FIGURE 4.5 Average monthly soil temperatures at a 50-cm depth in 8 beaches along the
Atlantic coast of Florida between Ft. Pierce Inlet and Boca Raton.
Island Cheionia mydas was negatively correlated with sand grain size. However,
there appears to be very little data on the water exchange of sea turtle eggs during
incubation. It seems to be generally accepted that eggs take up water during early
incubation and become more t urgid,but the quantity of water exchanged is unclear.
There is at least one reporU^ indicating that eggs may lose water near the end of
incubation, producing a reduction in clutch volume. There is very little available on
what happens in between. However, because the water in the sand surrounding the
clutch is likely to have a water potential on the order of -5 to -50 kPa and because
the water potential inside the egg is on the order of -900 kPa^^ “^^ there is a net
tendency of water to move into the egg during incubation. An added complication
for the understanding of egg water exchange is that sea turtle eggs are incubated in
large clutches rather than as single eggs. Eggs in the interior of the nest contact each
other and the spaces between because the interior of the clutch remains free of sand.
As the eggs take up water and swell, these spaces will shrink and disappear. Eggs
at the periphery of the nest contact sand over some fraction of their surface as well
as other eggs, a rather different physical situation. Moreover, clutches heat up by
several degrees during incubation with peak temperature occurring at the center of
the clutch. Temperature increases of this magnitude will have a significant impact
on egg water exchange.^
The general concepts of egg water exchange can be examined using a steady-
state model for the water exchange of a single egg in contact with soil:
92 The Biology of Sea Turtles
where M hzo (liigM) is the rate of water exchange, Lp (mg day~^ kPa” 0 is the
permeability of the eggshell and shell membranes, A (cm^) is the area of the eggshell
and Peh20 (kPa) and Psh20 (kPa) are the vapor pressures of the egg and sand,
respectively. The product, Lp A, is often described as the water vapor conductance
of the eggshell:
G hjO = Lp ■a (4.2)
where G h20 = irig day~^ kPa~k Egg water exchange as modeled by Equation 4.1 is
driven by a vapor pressure (vp) difference between the egg and the surrounding sand
which describes the direction of the exchange, while the magnitude of the water
exchange is given by the product of the vp difference and the conductance. It is
important to note^^ that the permeability represents the sum, in series, of the eggshell
permeability (Lp®) and the soil permeability (Lp"):
However, Lp" for moist sand is orders of magnitude greater than Lp®. As a result,
L ®becomes limiting^^ and Equation 4.3 reduces to:
Thus Equation 4.2 should describe the only important conductance for water vapor
exchange of the sea turtle egg. The G h20 of only Caretta caretta appears to have
been measured (2175 mg • day~^ • kPa This value was close to that expected
for other flexible-shelled reptile eggs and about 3 orders of magnitude larger than
for a similarly sized bird egg.'^^ '^^ The magnitude of the Caretta G h20 means that
the eggs should lose water at a high rate in an environment only slightly drier than
the egg i tself. One implication seems to be that the environment of sea turtle eggs
must be very humid. In any event, the magnitude of the vp difference between the
inside and outside of the egg takes on much greater significance for the sea turtle
than it does for a bird egg. To my knowledge, no one has reported values for the vp
(Peiho) of sea turtle eggs. We can assume that the egg vp is equivalent to typical
vertebrate tissue vp. Data for other reptile eggs indicate that this assumption is not
unreasonable.^^’^^ It is possible, however, that Peh20 changes with incubation time
due to osmotic processes occurring inside the egg.^^ "^^Moreover, Peh20 is a function
of egg temperature and will change with time as the temperature of the egg changes
during development.^’^^
Sand vp (Psii20 ) at the other end of the vp difference driving egg water exchange
is a function of several variables. Psh20 is determined chiefly by the relationship
The Nest Environment and the Embryonic Development of Sea Turtles 93
between the water content and water potential ('F) of the sand and is a function of
sand water content. Dry sand is comprised of solid sand grains and gas. When water
is present, gas is displaced until the water completely fills the gas space. The sand
is then considered to be saturated with water. At saturation, the hydraulic pressure
in the water is zero with reference to atmospheric pressure. As the water content of
the soil decreases from saturation, a negative pressure occurs in the soil water. This
pressure is often described as a water potential ('F, the potential energy per unit
volume, J • m"^) whose dimensions are equivalent to those of pressure (kPa) and
can be generated in several ways.^'^'^'^ ( 1 ) T' is a matric potential if it is due to the
capillary effects of surface tension in soil pores or to the binding of water to the
surface of soil particles.^^ This is a negative potential. As the soil dries, becomes
increasingly negative. A characteristic curve describes graphically the relationship
between sand water content and 'F of water. (2) An osmotic potential may be present
in the water if solutes such as salt are present. This is also a negative potential that
is proportional to the concentration of solute present. (3) If the liquid water in the
soil is connected hydraulically to a saturated water source (the water table), as is
likely to be the case for a nesting beach, then a gravitational potential is also present.
This potential is positive and is equivalent to 10 kPa for each meter above the water
table. The total potential in sand water is thus the sum of the matric (-), osmotic
(-), and gravitational (+) potentials. In terms of water transport in the sand, the
matric potentials tends to hold water in while the gravitational potential tends to
cause water to drain out. The vp of water in the soil gas phase is generally assumed
to be in equilibrium with 'F. Relative humidity (H,., %) can be related to water
potential (T^, 1 kPa = 1 J • kg"0 by:
= (R . T / MJ • ln(H,) (4.5)
where the gas constant R is 8.31 J • mok^ • the molar mass of water, M^, is
0.018 kg • mok’ and T is temperature (°K). Characteristic curves for sand from a
number of nesting beaches are shown in Figure 4.6. Only a limited range for W is
shown because totally dry sand is assumed to have a water potential of - 1 ,0 0 0 , 0 0 0
kPa.'^'’ Sand loses cohesiveness at 'F greater than -50 to -100 kPa and can no longer
support nest excavation. The horizontal section of the characteristic curve represents
the presence of bulk water in the sand. The vertical section of curve represents water
bound to the surface of the sand particles. Bound water remains after the bulk water
has drained from the soil."^^ When bulk water has been removed, leaving only bound
water, sand loses its cohesiveness and the individual particles no longer stick
together. The sand appears to be dry. When the external pressure exceeds the capillary
pressure holding water in the soil, the soil pores empty. When bulk water is present
in the soil, 'F is only weakly dependent on soil water content. This is pronounced
for sand (Figure 4.6), where water potential is still above -10 kPa even though most
of the water has been lost. I have data for sands from 16 different nesting beaches.
All release their bulk liquid water at matric water potentials less negative (lower)
than -10 to -15 kPa. In sands, the pores are relatively large and of similar size so
that emptying (or filling) occurs abruptly as matric forces are exceeded by drying
(or wetting) forces, but the characteristic curves are dependent to some extent on
94 The Biology of Sea Turtles
sand grain size. More coarsely textured sands will hold water less tightly than more
finely textured sands because the pores are larger in the coarser sands. This means
that coarsely grained sands will empty at lower matric water potentials than more
finely grained sands.
cd
CL
c
CD
■*—*
o
a.
o3
cd
cd
The vp and present in the beach sand depends fundamentally on the quantity
of water in the sand and its distribution in the beach. The distribution of water in
the beach is a result of the balance between water entering and leaving a layer of
sand at any depth in the sand depth profile. If the distribution is stable it is because
on the average, the same quantity of water enters a layer as leaves it. Sea turtle
nesting beaches are characterized by what appear to be relatively stable distributions
of beach water that are far from equilibrium and dry out only slowly in the absence
of rain.^^ The elements of a water balance for a beach are presented by Ackerman.^^
Nesting beaches are likely to have a source of liquid water (a water table) present
not far from the surface (1-5 meters). The water table is close enough to the surface
to generate a gravitational potential in the beach. Water will drain downward toward
the water table, but slowly because the 'T difference driving water movement is
small (on the order of a few kPa). The chief source of water in the beach is rain.
Water injected by rain enters the beach and drains down its gravitational field until
it reaches equilibrium with the capillary forces (the matric water potential). Thus at
1 m above the water table the equilibrium matric potential would be -10 kPa,
offsetting the 10 kPa gravitational potential and producing a total potential of 0 kPa.
The Nest Environment and the Embryonic Development of Sea Turtles 95
Note that sand with a NKof ~10 kPa is not dry and still retains 1 to 2% water. The
equilibrium condition is approached slowly because the potential differences are
usually small, as demonstrated by the Caretta nesting beaches in Israel where no
rainfall occurs during the nesting season. When drying at the surface occurs after
a rainfall, water is lost from the soil to the atmosphere.^"^’^^"^^As water is lost at the
surface, it is drawn up from the underlying sand and the water potential of the sand
falls below its equilibrium point. Water is, in turn, pulled up from the free water
surface further down in the sand column. Water transport in the soil column produces
a characteristic profile in which, after a period of drying, a layer of dry sand comes
to overlie a layer of wetter sand. This distribution is familiar to anyone who has
worked on a sea turtle nesting beach where a layer of dry sand always overlies
moister sand underneath (in the absence of rain). The top, dry soil layer limits water
loss to the atmosphere from the wetter layer. Near equilibrium, liquid water may be
drawn up at a rate sufficient to balance the water lost across the suiface.^'^’^^'^^ The
evaporation from the surface is self-limiting. This is because the rate of water vapor
transport decreases as the length of the diffusion path increases. It is also because
the density of vapor in the pathway decreases substantially after short periods of
time.^'^’^^ The thickness of the dry layer will depend, among other things, on the time
since the infiltration of surface water, on the sand structure, and on the depth of the
water table. Given sufficient time after surface infiltration, an equilibrium distribution
may be reached, but this is likely to require weeks to months.^^ Thus in Israel, with
no rain during the nesting season, it appears to take in excess of 3 months to approach
equilibrium.^^ deeper the liquid water surface, the thicker the dry layer is likely
to be. It is also likely to be thicker in coarser sand than in finer sands because coarse
sands hold water less tightly than finer sands. This phenomenon may account for
the observation by Mortimer^^ that hatching success was lower in more coarsely
grained Ascension Island beaches. These beaches appeared to be drier than the more
finely grained beaches. Most likely, drying fronts had penetrated much more deeply
in the coarser beaches, resulting in a much drier environment for the eggs, perhaps
dry enough to produce an increase in egg mortality.
Reports of water content profiles in sea turtle nesting beaches are not extensive.
Ackerman^^ presented a profile for an Israeli beach taken near Caretta nests. Figure
4.7 presents the average water content in five beaches along the Atlantic coast of
Florida.'^^ The profiles appear as described above with a dry layer at the surface, an
intennediate layer with increased water content, and wet layer at the intersection of
the soil column with the water table. The moist intermediate layer is characterized
by an essentially constant water content throughout its depth. The water content was
around 4 to 6 % (by mass) and was far from the saturation values of around 35 to
45%. It is in this layer that sea turtle eggs are placed. The most important difference
between the Florida and Israeli beaches is in the frequency of rainfall. In Florida,
rain often occurs daily and weekly during the nesting season. The result is that the
water content of Florida beaches is higher throughout the season, while the water
content in Israel starts out high (but lower than in Florida) and decreases during the
nesting season as gravitational drainage occurs. Moreover, a drying front forms in
both locations, but is much deeper in the Israeli beach (15 to 20 cm) than in the
Florida beaches (5 to 10 cm). In both locations, the dry sand at the surface has a ¥
96 The Biology of Sea Turtles
FIGURE 4.7 Water content profile for 5 beaches along the Atlantic coast of Florida. The
beach surface is at 0-cm depth. Water table is typically in excess of 1 m.
of around -100,000 kPa or more while the matric T in the intermediate layer is
around -5 to -10 kPa. The drying front where the transition from dry sand to moist
sand occurs is around 5 cm wide and has a ^ gradient on the order of 10,000 to
20,000 kPa • cm~h The presence of salt in the water will increase T somewhat.
Mortimer^^ and Rimkus et al."^^ report low levels of salt in beach water producing
osmotic potentials on the order of -10 to -60 kPa. These represent very low salt
concentrations because seawater has a water potential of around -2000 kPa. Rainfall
is likely to flush salt from beach water rapidly. Rimkus et al.,"^^ studying the effect
of beach renourishment on beach water, found that beaches renourished with sand
in a seawater slurry lost all their salt in two weeks or less. Average total (matric
plus osmotic potential) is shown for some Florida beaches in Figure 4.8. Total is
likely to be higher in locations such as Israel, where there is much less rainfall.^^^
It appears that the W and therefore the water vp in the sand around the sea turtle
clutch is relatively constant throughout the typical incubation period. If we assume
a sand temperature of 30°C and take a typical 'F to be around -30 kPa, then the
water vp in the sand is around 4.232 kPa. If the osmotic concentration of the egg
is typical of vertebrate tissue, then the water vp of the egg is around 4.207 kPa. The
water vp difference across the eggshell is around 0.025 kPa. Substituting this value
and the value for G h20 (2175 mg • day~^ • kPa~0 into Equation 1, we find that a
typical egg would take up around 54 mg day“^ of water vapor. Over 60 d, this is
around 3.3 g or about 6.5% of the mass of a 50-g Caretta egg. This water uptake
should be considered the maximum quantity possible because the eggs are incubated
in clutches rather than singly and because the clutch heats up. Eggs at the periphery
The Nest Environment and the Embryonic Development of Sea Turtles 97
FIGURE 4.8 Total water potential (matric plus osmotic) as a function of depth for beaches
along the Atlantic coast of Florida. Data are averaged for 7 beaches. The increased variation
at depths greater than 80 cm is due to proximity to the water table which had a salinity typical
of seawater.
of the clutch will be closer to this value than eggs at the center of the clutch. Indeed,
eggs toward the center will heat up sufficiently to reverse the direction of water
vapor transport. An increase in egg temperature of 0.1 °C is sufficient to bring egg
water vp to the same level as sand water vp. Pearson"^^ analyzed the effect of clutch
position and heating on egg water exchange. His model indicated that eggs at the
periphery of the nest should experience a net water uptake and eggs at the center
of the nest a net water loss during incubation. Water loss for the central eggs occurred
near the end of incubation. The observation^'^ of a decrease in egg volume near the
end of incubation is consistent with the Pearson"^^ analysis.
of sea turtle eggs. Net diffusion of any gas can only occur when there is a concen
tration difference for the gas. This means that, in order to supply the O2 or get rid
of the CO2 associated with developmental metabolism, concentration differences
must set up between the center and edge of the egg clutch and between the edge of
the egg clutch and the surrounding sand. As egg and clutch metabolism change with
time during incubation, so too should the concentration differences which occur to
supply the respiratory needs of the clutch. Ackerman'^^ measured P02 and Pco2
the sand surrounding the clutch and inside the clutch of C. mydas and Caretta caretta
nests in Costa Rica and Florida. Pq, decreased in the center of the clutch from near
atmospheric levels at the beginning of incubation to around 12 to 14 kPa near the
end of incubation, while Pco, increased to around 4 to 6 kPa near the end of
incubation. Maloney et al.^'^ reported similar patterns of change, but smaller magni
tudes in Caretta nests in Queensland. Ackerman"^^ used a one-dimensional, spherical
diffusion model to quantitatively describe gas change by sea turtle clutches, treating
the medium (the beach) as if it were infinite. Among other things, this implies that
clutch gas exchange is independent of the beach surface. However, Maloney et al.^'^
concluded that the model used by Ackerman"^'^ was not adequate to describe clutch
gas exchange in their location. In the situation described by Maloney et al.,^‘^ the
surface may well have influenced clutch gas exchange. This would mean that gas
exchange through the top of the nest would have a disproportionate influence on
nest gases. The modeP^ can be modified easily to deal with this by adding dimen
sions. An explanation for the observation of Maloney et al.‘^ may lie in the relation
ship between the gas diffusivity of the sand, its water content, and the distribution
of water in the sand above the clutch (as described earlier). The gas diffusivity of
sand (D ) can be approximated by the relationship
(4.6)
where is the gas diffusivity in air and (j)g is the fraction of the sand that is air
(taking into account the water present). More accurate estimators are available if
required.^"^ As described above, a dry layer of considerable thickness is usually found
at the beach surface. This layer will have a greater than the moist underlying
layers where the water content is on the order of 2 to 10%. Egg clutches incubating
in drier climate will tend to be closer to the dry layer (i.e., the dry layer will penetrate
into the beach more deeply) than egg clutches incubating in wetter climates. The
increased thickness of the dry layer should (at some point) increase the contribution
of gas exchange through the top of the nest to overall nest gas exchange. For locations
where extensive surface drying may occur, such as Israel or Queensland, a radial
(one-dimensional) gas exchange model might not account well for clutch gas
exchange, and a two-dimensional model might be more suitable. It is also worth
considering the influence of nest crowding on clutch gas exchange. The model used
by Ackerman"^'^ assumed that each nest in the beach was independent of all other
nests; that is, that nests were not “breathing” each other’s gases. When nests are
crowded together, as, for example, may occur in hatchery situations, the gas exchange
of nests will not be independent of each other. It is not clear what crowding means
quantitatively, but if nests are within a meter or less of each other then it is likely
The Nest Environment and the Embryonic Development of Sea Turtles 99
that the influence of one nest on another must be taken into account. As crowding
increases, then clutch gas exchange through the top or bottom of the nest becomes
progressively more important than gas exchange through the sides, and a two-
dimensional planar model may become more applicable.
Clutch gas is important because it establishes the background concentrations of
respiratory gases in which the individual egg must exchange the gases necessary to
support the metabolic activity of the developing embryo. Gas exchange between the
clutch gases and blood in the chorioallantoic capillaries of the egg occurs across the
eggshell and associated eggshell membranes. This gas exchange process has been
reviewed by Deeming and Thompson^^ for reptile eggs, but has not been extensively
studied. Ackerman and Prange^^ reported the O2 diffusion coefficient for Chelonia
mydas eggshell and outer shell membrane to be around 5 x 10"^ cm^ s^^ cm“^kPa ^
a magnitude about twice that of a similarly sized chicken egg. Because the O2 uptake
of the sea turtle egg is only about 2 0 % that of the chicken egg, the O2 difference
across the eggshell and membrane must be much smaller than for the chicken egg.
An interesting aspect of this is that the diffusivity of the sea turtle shell must be a
function of its water content. When the egg is oviposited, the shell is flooded with
fluid. This fluid, if allowed to remain in the shell, would present such a large
resistance to O2 transport (given the shell thickness) that the embryo would run out
of O2 well before the end of incubation. Therefore, the shell must be dried out in
some may; furthermore, as water is taken up by the egg during incubation, that water
cannot be allowed to accumulate in the eggshell. The relationship between egg gas
exchange and egg water exchange is unexplored.
no influence on the nearshore processes which eroded the beach in the first instance
(or on sea level increases), it generally has to be repeated at intervals. It is clear that
the process of renourishment produces a beach that is different in some ways from
natural beaches. In particular, renourished beaches appear to be harder than natural
beaches and a vertical, erosional scarp often forms in the surface zone of the beach.
It has been argued that this is due to compaction of the new beach m a t e r i a l . T h e
continued use by sea turtles of the renourished beaches for egg incubation has
generated concern that beach renourishment might adversely influence sea turtle
reproduction. This is of some concern because, as we have discussed above, it is
known that the incubation climate critically influences embryonic development in
sea turtles and other reptiles. Moreover, beach hardening may well impact nest
excavation and structure as well as emergence from the nest of hatchlings. However,
there is little information available on how renourishment might change the climate
for development in the beach.
Rimkus et al."^^ examined the hydric properties of eight renourished and seven
natural beaches along the Atlantic coast of Florida in order to provide adequate
replication for analyzing treatment effects. Although the distribution of water in both
types of beach were similar, renourished beaches appear to retain more water than
natural beaches. The field capacity^^’"^^ of renourished beaches was greater than that
of natural beaches. The difference in water retention (i.e., increased water content)
is consistent with a difference in the characteristic curves of renourished and natural
sand. Renourished sand appears to hold slightly (but significantly) more water at
the occuiTing in the sand. The difference in water retention may be attributable
to differences in the sorting of sand composing the beach. The natural process of
beach formation results in extensive sorting of the sand both by layers and within
layers.^^ The orientation of the layer is determined by the wave wash on the beach
and is typically inclined. Renourished beaches are unlikely to be sorted in the same
way because they are not constructed in the same way.
Renourished beaches had a lower bulk density and were less compact than
natural beaches."^® This finding was not consistent with earlier work where the reverse
was reported.^^"^^ However, the earlier findings were not based on measurements of
bulk density; rather, increased compaction was inferred from measurements of beach
shear resistance using cone penetrometers.^® Increases in shear resistance can be due
to increases in compaction, but they may also be due to other factors such as sand
particle shape and surface characteristics and to interactions (e.g., chemical) between
particles.®^ Scarp formation can be attributed to an increase in the shear resistance®^
rather than to beach compaction and thus the presence of scarps does not confimi
that a beach is compacted. Another explanation needs to be sought. A measure of
beach shear resistance can also be described as a measure of beach hardening or
strength. Shear resistance describes the ability of a soil, in this case, beach sand, to
resist sliding along internal surfaces. In addition to the external force operating on
the object, the object exerts a normal force on the surface over which it is tending
to move. For a beach, the nonnal force would be the weight of sand (overburden)
pressing down at the point of movement. The force required to just cause the surfaces
to slide along each other (the point at which the material fails) is called the shearing
The Nest Environment and the Embryonic Development of Sea Turtles 101
stress (Tf) and is related to the angle of stress of the normal force (a) and the friction
angle ((|)) by the equation:
For soils, 0 is defined as the angle of internal friction. This angle represents the sum
of the sliding friction ((j)^) and the effect of interlocking by the particles (9):
+ e (4.8)
where an additional variable, cohesion (c), has been added to Equation 4.7. This
relationship can be deteimined in the laboratory. However, another factor must also
be considered. The new material used to renourish beaches may interact chemically
through organic molecules bound to the surface. This could also result in increased
shear resistance and should be evaluated. The scarps observed on renourished
beaches in the tidal wash zone of the beach can be of considerable height and can
be dangerous to humans, while limiting sea turtle access to the beach. Increased
shear strength acts to increase the stability of slopes^^ with the result that scarps
should be more stable on renourished beaches which exhibit increased shear resis
tance. Vertical cuts, such as scarps on beaches, are common in gullying or erosional
situations. The height of the scarp (HJ can be calculated from the angle of internal
friction ((|)) and the cohesion (c). To a first-order approximation, the greater the (|)
the greater the height (HJ of the scarp that can develop:
where y is the unit weight of sand. Thus the magnitude of scarp formation on a
renourished beach could serve as an index to changes in shear resistance of the
beach if compaction were measured in some independent way.
102 The Biology of Sea Turtles
in the center of the clutch. The biological relevance of this potential effect remains
to be investigated, but it has been shown experimentally that sea turtle eggs are
sensitive to altered respiratory gas exchange.^’^"^
ACKNOWLEDGMENTS
This work has been supported by grants from the Israel-U.S. Binational Science
Foundation, the National Science Foundation, and the Florida Department of Natural
Resources. Amos Ar, Razi Dmi’el, Robert Horton, Richard Seagrave, and William
Bardsley have contributed greatly to my work and my education.
REFERENCES
1. Miller, J. D., Embryology of marine turtles, in Cans, C., Billett, F., and Maderson,
R F. A., Eds., B io lo g y o f th e R e p tilia , D e v e lo p m e n t A, Vol. 14, John Wiley & Sons,
New York, 1985, 269-328.
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106 The Biology of Sea Turtles
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C Orientation, Navigation,
and Natal Beach Homing
in Sea Turtles
Kenneth j, Lohmann, Blair E, Witherington,
Catherine M. F. Lohmann, and Michael Salmon
CONTENTS
0-8493-8422-2/97/$0 00+$.50
© 1997 by CRC Press, Inc. 107
108 The Biology of Sea Turtles
5.1 INTRODUCTION
The lives of sea turtles consist of a continuous series of migrations. As hatchlings,
the turtles swim from their natal beaches into the open sea,^ "*often taking refuge in
circular current systems (gyres) that serve as moving, open-ocean nursery grounds."^
The juveniles and subadults of many populations reside in coastal feeding areas that
are located hundreds or thousands of kilometers from the beaches on which the
turtles hatched.“’'^ Adult females, however, periodically leave their feeding sites and
migrate to their natal beaches to nest; after nesting, many return to their own specific
feeding areas.^ The itinerant lifestyle that characterizes sea turtles is thus inextricably
linked to an ability to orient and navigate accurately across large expanses of
seemingly featureless ocean.
In some sea turtle populations, migratory performance reaches extremes. The
total distances certain green turtles (Che Ionia my das) and loggerheads (Carena
carena) traverse over the span of their lifetimes exceed tens of thousands of kilo
meters — several times the diameter of the home ocean basin of the turtle.^ Adult
migrations between feeding and nesting habitats can require continuous swimming
for periods of several weeks.^’^^ In addition, the satellite tracks of migrating turtles
often lead straight across the open ocean and directly to the de s t i na t i on. The s e
position plots leave little doubt that adult turtles know where they are going.
The migrations of hatchling turtles are no less impressive. Within seconds of
emerging from their underground nests at night, hatchlings begin to crawl directly
toward an ocean that cannot be seen from the emergence site. The young turtles
enter the sea and immediately establish offshore headings, which are maintained
long after the hatchlings swim beyond sight of land.^’^’^^ Thus, even as hatchlings,
sea turtles are finely tuned migratory machines equipped with an array of mecha
nisms for guiding themselves across large expanses of open ocean.
For logistical reasons, most studies on orientation mechanisms in sea turtles
have focused on hatchlings rather than adults. Adults, after all, are powerful animals
that often exceed one hundred kilograms in weight; they are also difficult to keep
in captivity, and migrate only intermittently. In contrast, hatchlings are small, sea
sonally abundant, easy to manipulate in laboratory and field studies, and strongly
motivated to migrate offshore. In recent years, considerable progress has been made
in characterizing the orientation cues that guide hatchling turtles during their offshore
migration.
In this chapter, we begin by summarizing the orientation mechanisms used by
hatchling turtles, first on land as they move from their nests to the sea, then in the
ocean as they migrate offshore. We then review recent experimental evidence that
Orientation, Navigation, and Natal Beach Homing in Sea Turtles 109
hatchlings can detect magnetic field inclination angle and magnetic field intensity,
two geomagnetic features that vary across the surface of the earth and may provide
sea turtles with information on their global position. We conclude by reviewing
hypotheses of adult navigation and possible mechanisms by which adults might find
and recognize their natal beaches after spending years in distant oceanic regions.
Sea turtle clutches usually contain between 50 and 170 eggs^^ and develop in nests
buried beneath the sand of oceanic beaches. After the eggs hatch, the hatchlings
must ascend to the beach surface from the underground egg chamber. Although this
upward journey is rarely more than about 1.0 m in distance, it can nevertheless be
difficult, particularly when the sand above the eggs has become compacted or
overgrown with plant roots. The emergence of hatchlings onto the beach is thus the
culmination of a considerable amount of activity that occurs underground after the
turtles hatch.
The most detailed accounts of hatchling behavior within the nest come from
observations on loggerheads^^ and green t u r t l e s . I n these species, most turtles
within a clutch pip (i.e., cut through the eggshell) within a few hours of each other.
After pipping, hatchlings remain quiescent for approximately one d, often with their
heads and front flippers protruding from the egg. During this time yolk is resorbed
into the body cavity and the plastron straightens. In addition, an airspace begins to
form in the upper egg chamber as fluid drains from pipped eggs into the surrounding
sand. This drainage reduces the volume that the eggs occupy and creates sufficient
space in the nest for subsequent hatchling movements.
After the quiescent period, hatchlings extricate themselves from their eggshells
and begin exaggerated crawling m ovem ents.This activity is synchronized among
siblings and occurs episodically in bouts that last several hours. Activity is elicited
by contact with moving siblings and inhibited by high temperatures (as occur during
mi dda y) . Soc i a l l y facilitated upward movement of the hatchling mass occurs as
turtles near the top of the cavity scrape sand from the ceiling of the chamber, while
those below trample it into the floor as they struggle to crawl upward. Thus, the
chamber containing the hatchlings gradually ascends until at last the turtles break
through the surface of the sand.
Hatchlings emerge en masse from the nest 1 to 7 d after pipping ^^^2 imme
diately crawl seaward. Each emergence usually involves between 20 and 120
hatchlings.^^ Emergence marks the beginning of the “hatchling frenzy,” a period of
high activity during which hatchlings enter the sea and swim away from land. Turtles
that pip late or are less active take part in smaller emergence events on subsequent
evenings, or may be unable to escape from the nest at all.^^
Because lower sand temperatures that occur after sunset elicit an increase in
hatchling activity, most hatchling emergences occur at night.^^’^^ Nevertheless, some
early morning emergences^'^ and diurnal emergences during rain storms^'^ also occur.
Among loggerhead nests, emergence activity has a relatively normal temporal
110 The Biology of Sea Turtles
distribution, with a peak near midnight^^ (Figure 5.1). Emergence at night allows
hatchlings to cross the beach at a time when risks of hyperthermia and predation
are relatively low.
LU
2 CO
LU tZ
0 LU
QC >
LU in
LU
TIME
FIGURE 5.1 The timing of 157 loggerhead emergence events from natural nests. An
emergence event was defined as the movement of 10 or more hatchlings from a nest to the
sea. (From Witherington, B. E., Bjorndal, K. A., and McCabe, C. M.,Copeia, 4, 1165, 1990.
With permission.)
FIGURE 5.2 Orientation responses to colored light of hatchlings from each of four species
of sea turtles. Turtles were tested in a modified Y-maze that contained two windows, one of
which was illuminated with light of a specific wavelength. Responses were measured as the
proportion of hatchlings (of 30 tested) that chose the illuminated window over an alternative
darkened window. A single quantum radiance at approximately full-moon irradiance (1.58 x
10"^ photons/s/m7nm) was used for the colored source. Aversion indicates that hatchlings
preferred the dark window to the lighted window. Indifference is the statistically random
choice of windows according to a binomial probability test at a = 0.05. (From Witherington,
B. E., Sea-Finding Behavior and the Use of Photic Orientation Cues by Hatchling Sea Turtles,
Ph.D. dissertation. University of Florida, Gainesville, 1992.)
Thus, whereas ERG data indicate that green turtles are highly sensitive to yellow
light,this does not imply that they are strongly attracted to it.
Xanthophobia in loggerheads only occurs at relatively high light intensities and
is probably dependent upon photopic (cone) vision."^^ To assess responses to low-
intensity light, the threshold intensity required to elicit a statistically significant
orientation response in loggerhead hatchlings was determined for several different
wavelengths (Figure 5.3). At lower light levels, turtles oriented toward the light
source at all wavelengths studied, including yellow. Ultraviolet-green light required
the lowest intensity to elicit orientation and was thus the most attractive to the
Orientation, Navigation, and Natal Beach Homing in Sea Turtles 113
>-
>
LU
CO
-J
<
OC
5
X
LU
CD
WAVELENGTH (nm)
hatchlings. The threshold intensity for yellow light was about one order of magnitude
higher, and that for red light about five orders of magnitude higher, than the threshold
intensities for the shorter wavelengths.
Such changes in sensitivity and orientation behavior in response to yellow light
may have a proximate explanation based upon loss of color discrimination at low
light intensities. The adaptive significance of these responses in hatchlings may be
to factor out light from bright celestial sources near the horizon. A rising or setting
sun or moon is rich in yellow light, due to scattering effects of the atmosphere of
the earth. A bias against yellow would make these sources less attractive than they
otherwise would be.
According to this hypothesis, the rising sun should have a minimal influence
upon orientation during sea-finding. Indeed, the sea-finding orientation of logger-
head, green turtle, and hawksbill hatchlings appears to be altered little or not at all
when the sun is close to the h o r i z o n . M i n o r influences of the sun on
orientation may be a function not only of color, but also of the way hatchlings
spatially integrate their measurement of brightness (see below).
114 The Biology of Sea Turtles
FIGURE 5.4 A hypothetical cone of acceptance for the assessment of brightest direction
by a sea turtle hatchling. The vertical component of the cone (V) is approximately 10 to 3CP
from the horizontal plane. The horizontal component of the cone (H) is approximately 180P.
Light sources within this cone of acceptance are integrated into a measure of brightness for
the direction D.
The horizontal component of the acceptance cone for green turtle,olive ridley,"^^
and loggerhead hatchlings^^ has been inferred from studies of hatchling orientation
in controlled light fields. In these studies, hatchlings were placed within a vertically
striped cylinder in which the direction perceived as brightest depended on the width
of the turtles’ acceptance cones. The results indicated that hatchlings of each species
measured the brightest direction with a wide (about 180°) horizontal acceptance
cone (Figure 5.4). The vertical component was determined by analyzing responses
to light sources positioned at various vertical angles relative to the hatchling. The
vertical component has been estimated to be “a few degrees” for green turtles and
olive ridleys"^^ and between 10° below and 30° above the horizon for loggerheads.
The vertically narrow acceptance cone for hatchlings means that light closest to
the horizon plays the greatest role in determining orientation direction. The integra
tion of brightness over a horizontally broad acceptance cone means that irradiance
(light reaching the hatchling) is a more important consideration than radiance (light
emanating from a source). Integration of light over a broad range may also be
important for mitigating the effects of celestial light source position on orientation
Orientation, Navigation, and Natal Beach Homing in Sea Turtles 115
direction; a broad acceptance cone gives hatchlings a larger “sample”, which dimin
ishes the influence of any single point source of light.
always the brightest during clear, moonless nights, the brightest direction is not
always seaward when a full moon rises or sets.^^ Nevertheless, hatchlings released
at moonset and moonrise orient seaward without difiiculty^^.
As waves approach shore and break, the pattern of light reflected toward the beach
changes rapidly. If hatchlings can distinguish between the shimmering, constantly
shifting patterns of light reflected from waves and the more static light regime of
land, then the movement of light could play a role in guiding turtles seaward. Such
a possibility, however, has not yet been investigated.
The periodicity or flashing pattern of light (again, such as might be observed
from reflections off waves) might also serve as an orientation cue. However, evidence
suggests that green turtles do not distinguish between constant and flashing light
sources except when the off time of the flashing source is very long.^^ In this latter
case, turtles actually prefer a constant light source over the flashing one, a preference
which suggests that flashing patterns are not important in orientation. Moreover, the
avoidance of the slowly flashing source suggests that turtles are merely measuring
relative brightness of the two light sources and are integrating their measure of
brightness over time.
Because light reflected from water is usually polarized, polarized light might
also indicate the seaward direction. In orientation experiments, however, loggerheads
did not respond differently to polarized and unpolarized light.^^
Celestial light originates from intense, but distant sources. Such light is scattered
by the atmosphere and reflected by the surface of the earth. Natural light fields are
therefore only moderately directed. Artificial light fields, in contrast, are produced
by less intense, but closer sources; thus, they can be highly directed with one
direction much brighter than all others. Because artificial light sources are not intense
enough to brighten the remainder of the observer’s world, artificial light fields are
overwhelmingly dominated by the light sources that generate them.
Under natural conditions, hatchlings can use multiple cues to find the ocean.
Thus, if a single cue such as brightness does not precisely indicate the seaward
direction, it seldom overrides all other cues, and turtles are still able to locate the
sea. Artificial light sources alter conditions by creating a beach environment in which
extremes of brightness exist. To a hatchling on a dark beach, an artificial light source
may thus become a supernormal stimulus that unambiguously indicates the seaward
direction. At such high levels of stimulation, hatchlings may ignore shape cues and
other features of the beach, or perhaps not even perceive them.
The common misconception that hatchlings emerge only during the full moon
probably originated because hatchlings are most easily observed on bright, moonlit
nights. In fact, no evidence exists that hatchling emergences are correlated with lunar
phase. The date of emergence is determined by the date eggs were deposited in the
nest and by the length of the incubation period, which depends in part upon tem
perature. Although nesting cycles correlated with moon phase may occur in some
mass nesting (“arribada”) species (e.g., olive ridley)^"^ and in a few loggerhead
populations,^^ variation in incubation time and other factors typically results in
emergences occurring throughout the lunar month.
Moonlight does affect the degree of sea-finding disruption caused by artificial
lighting. Hatchling misorientation in areas with artificial lighting is most frequent
and severe on nights near the new moon.^^ Higher levels of ambient light, as occur
during other moon phases, diminish the directivity of artificial light fields on the
beach. Under such conditions, hatchlings may rely more heavily on shape and
silhouette cues to guide themselves seaward.
FIGURE 5.5 A summary of experiments in which green turtle hatchlings were released at
sea near the east coast of Florida, U.S.A., on days when waves moved in various directions.
Each data point represents the mean angle of orientation of one group of hatchlings plotted
with respect to the angle of wave approach at the time of release. Hatchlings in each
experiment swam into waves, even when doing so resulted in orientation toward directions
other than offshore. (From Lohmann, K. J. and Lohmann, C. M. F .,i. Exp. Biol., I l l , 1,
1992. With permission.)
Laboratory experiments have also confirmed that loggerhead, green turtle, and
leatherback hatchlings can use waves as a directional cue.^^’^^ When hatchlings were
tethered in a wave tank, they swam into waves when waves were present, but oriented
randomly in still water.
Orientation, Navigation, and Natal Beach Homing in Sea Turtles 119
Wave Direction
FIGURE 5.6 The motion of a hatchling turtle swimming with and against the direction of
wave propagation. See text for details. (From Lohmann, K. J., Swartz, A. W., and Lohmann,
C. M. R, J. Exp. Biol., 198, 1079, 1995. With permission.)
To determine whether or not hatchlings might detect wave direction in this way,
turtles were tested on a wave motion simulator that reproduced in air the circular
movements that a hatchling would normally encounter while swimming beneath
120 The Biology of Sea Turtles
Hatchlings that have just entered the ocean appear to orient exclusively on the basis
of waves; no evidence presently exists for the involvement of other directional cues
at this early stage of the offshore migration. Because waves entering shallow water
refract until they approach the beach directly, orienting into waves leads turtles
seaward. In deeper water farther from land, however, waves no longer provide a
reliable indicator of offshore direction. Evidence suggests, however, that hatchlings
may orient into waves for only a short time after entering the sea. Hatchling log
gerheads tracked from a Florida beach into the open ocean swam into waves initially,
but continued on the same seaward headings after entering offshore areas in which
wave direction no longer coincided with their established courses.*"'^ The ability to
maintain courses seemingly independent of wave direction implies that, after they
have distanced themselves from land, hatchlings use one or more alternative sources
of directional information to guide their movements.
O'* O'*
90 **
These results indicate that the position of light cues, or perhaps just the experi
ence of maintaining a course toward a specific direction, can influence subsequent
122 The Biology of Sea Turtles
while crossing the beach and swimming away from land is transferred to the mag
netic compass, so that hatchlings can continue on the same heading after swimming
beyond the wave refraction zone and entering the open ocean.
1. visual cues
Beach
FIGURE 5.8 Diagram summarizing the orientation cues hypothesized to guide hatchling
Florida loggerheads from their nests to the Gulf Stream current. The beach is to the left and
progressively deeper water is to the right. Lines represent oceanic waves moving toward the
beach; as they enter shallow coastal areas, waves refract until they approach the beach directly.
Visual cues guide hatchlings from their nests to the sea. Near shore, turtles swim into refracted
waves, which provide a reliable cue for swimming seaward. In deeper water, waves no longer
provide a consistent cue for offshore orientation, and hatchlings are hypothesized to transfer
their seaward courses to their magnetic compasses.
On the beach, hatchlings crawl seaward by orienting toward the brighter, lower,
oceanic horizon. Once in the ocean, turtles initially swim offshore by orienting into
waves, apparently without regard to visual cues. By the time hatchlings swim beyond
the wave refraction zone, they have probably transferred the initial seaward heading
to their magnetic compasses, enabling them to maintain offshore courses long after
swimming beyond sight of land. Because detailed studies on orientation mechanisms
have been carried out only with loggerhead hatchlings from the east coast of Florida,
whether or not the mechanisms outlined above are generally applicable to other
populations and species is not known.
124 The Biology of Sea Turtles
60 ° 30°
0° 0®
90" 90"
FIGURE 5.9 Orientation of hatchlings tested in magnetic fields of the same intensity, but
different inclinations. Conventions as in Figure 5.7. Turtles exposed to a 6CF inclination angle
(found near the northern edge of the North Atlantic gyre) were significantly oriented toward
the south-southwest, whereas those exposed to a 3(T inclination angle (found near the southern
border of the gyre) swam in a northeasterly direction. (From Lohmann, K. J. and Lohmann,
C. M. F., J. Exp. Biol., 194, 23, 1994. With pemiission.)
of chemicals in water,"^*’^^ but the role that chemical cues play in long-distance
navigation and natal beach recognition remains almost entirely unknown.
Even if chemical cues play no role in long-distance navigation, they may still enable
nesting turtles to recognize their natal beaches after other navigational mechanisms
have brought the turtles into close proximity. Some limited evidence exists to support
this hypothesis, but the results are not yet fully conclusive.
In one study,‘^'^ Kemp’s ridley turtle eggs from Rancho Nuevo, Mexico were
incubated in sand from Padre Island, TX. After emerging from their eggs, hatchlings
were permitted to crawl across the Padre Island beach and swim through the surf,
whereupon they were recaptured and held in captivity for four months. These juvenile
128 The Biology of Sea Turtles
turtles were then tested in a water-filled arena which contained four compartments
that the turtles could enter. One compartment contained a solution made from Padre
Island sand and seawater, one contained a similar solution made from sand and sea
water from a different location (Galveston, TX), and two contained untreated sea
water. The time that turtles spent in each compartment after entering was monitored.
Results indicated that turtles spent significantly more time per entry in the compart
ment containing Padre Island water than they spent in any of the other compartments.
Although these results are suggestive, all turtles tested in this initial study had
been “imprinted” to Padre Island sand and water; no attempt was made to test the
responses of turtles that had been similarly “imprinted” to water from another
location. Thus, the possibility that the turtles simply preferred Padre Island water to
the alternatives for reasons unrelated to early experience could not be excluded. A
second experiment was therefore carried out to determine whether turtles
“imprinted” to either Padre Island or Rancho Nuevo preferred water from their
respective natal b e a c h e s . N o such preferences could be discerned, but poor health
of the turtles may have adversely affected their performance.
In a subsequent experiment, green turtle eggs were incubated in sand that was
scented with one of two chemicals (morpholine or 2-phenylethanol). After hatching,
each turtle was held for an additional three months in water containing the same
chemical to which the turtle had been exposed as an embryo. After two additional
months without exposure to either chemical, the turtles were tested in the monitoring
tank, which this time contained solutions of morpholine, 2~phenylethanol, and
untreated sea water. Turtles that had previously been exposed to morpholine preferred
morpholine to 2-phenylethanol, whereas the opposite was true for turtles that had
been exposed to the 2-phenylethanol. Interestingly, however, additional groups of
turtles that had been exposed to the chemicals only while in the nest (for about 2
months) or only after emerging from the nest (i.e., while living in the water of the
holding tank for 3 months) failed to manifest these preferences.
The results demonstrate that, during certain developmental periods, turtles can
acquire a preference for specific chemicals and retain it for at least two months. The
significance of this finding, however, is not yet clear. Only turtles exposed to the
chemicals for a total of about 5 months (approximately 2 months in the nest, followed
by 3 months in water) acquired the preference. Under natural conditions, hatchlings
migrate beyond the waters of their natal beach within a few hours after leaving their
nests.^’^’^^ Thus, if turtles require several months of exposure to natal beach water
after hatching (in addition to exposure as embryos) for chemical imprinting to occur,
it is difficult to envision how such a process could occur under natural conditions.
Nevertheless, the initial results are intriguing, and additional studies are clearly
needed.
Animals with such an ability are said to have a map sense.Although the nature of
the sea turtle map remains unknown, a new hypothesis proposes that turtles determine
position using geomagnetic parameters.
That sea turtles can derive at least some positional information from features of
the magnetic field of the earth now seems c l e a r . A t a minimum, such information
appears likely to provide an estimate of latitude; in the case of Florida loggerheads,
it may assist young turtles in remaining within the borders of the North Atlantic
gyre.i>82 ability of turtles to detect both field inclination and intensity, however,
raises the possibility that adults use a bicoordinate magnetic map to pinpoint their
global position.
The migration of Ascension Island green turtles provides one intriguing example
of how such a bicoordinate magnetic map might permit navigation over a large
oceanic region. Isoclinics (lines of equal inclination angle) and isodynamics (lines
of equal field intensity) form a nonorthogonal grid between South America and
Africa^^ (Figure 5.10). Thus, all locations between feeding grounds in Brazil and
nesting beaches at Ascension Island are defined by unique combinations of inclina
tion and intensity. A migrating turtle using a bicoordinate magnetic map based on
these two parameters could therefore determine its position anywhere along its route,
provided it had learned the magnetic features of Ascension and the gradients of field
inclination and intensity in the South Atlantic.
FIGURE 5.10 Isoclinics (dotted lines) and isodynamics (solid lines) in the oceanic region
surrounding Ascension Island. Adjacent isoclinics differ by 2° and adjacent isodynamics by
1000 iiT. The two geomagnetic features form a nonorthogonal grid that might, in principle,
provide Ascension Island turtles with a bicoordinate position-finding system as they migrate
between Ascension and the Brazilian coast. (From Lohmann, K. J. and Lohmann, C. M. F.,
Nature, 380, 59, 1996, With permission.)
130 The Biology of Sea Turtles
if a magnetic map is used at all by turtles, it might range from a system that
provides only a crude estimate of global position to a highly developed sensory
system capable of pinpointing a specific beach. The resolution would presumably
depend on both the uniformity of the magnetic gradients over a given oceanic region
and on the sensitivity of the turtles to the two parameters. Although nothing is yet
known about the resolution with which turtles can detect specific magnetic features,
it is interesting to note that bees can detect magnetic anomalies in which the field
intensity differs from that of the local field of the earth by as little as 26 A
sensitivity of approximately 10 to 30 nT has been hypothesized for b i r d s . I f
turtles have similar abilities to discriminate intensity and can also detect inclination
angle with great precision, a navigational system capable of pinpointing specific
islands and beaches is at least theoretically possible. Alternatively, however, a mag
netic map might function only to guide turtles into the general vicinity of a nesting
beach, close enough for other cues (for example, chemical or visual) to take over
and allow final localization of the nesting site.
Although such speculation is inviting, whether or not turtles do indeed use a
bicoordinate magnetic map sense during long-distance migrations remains to be
determined. At present, the open-ocean navigational abilities of sea turtles remain
an enduring mystery of behavioral biology.
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ridley sea turtle, in Proc. 1st hit. Symp. on Kemp’s Ridley Sea Turtle Biology, Con
servation and Management, Caillouet, C. W., Jr. and Landry, A. M., Jr., Eds., Texas
A&M University Sea Grant College Program, TAMU-SG-89-105, 1989, 90.
101. Grassman, M. and Owens, D., Chemosensory imprinting in juvenile green sea turtles,
Chelonia mydas, Anim. Behav., 35, 929, 1987.
102. Walker, M. M. and Bitterman, M. E., Honeybees can be trained to respond to very
small changes in geomagnetic field intensity,/. Exp. Biol., 145, 489, 1989.
103. Gould, J. L., The map sense of pigeons. Nature, 296, 205, 1982.
6 Migration
Habitat Utilization and
in Juvenile Sea
Turtles
John A. Musick and Colin J. Limpus
CO N TEN T
6.1 Introduction.....................................................................................................137
6.2 Methods......................................................................................................... 139
6.3 Early Juvenile Nursery Habitats................................................................... 140
6.3.1 Loggerhead, Caretta caretta..............................................................140
6.3.2 Kemp’s Ridley, Lepidochelys kempi................................................. 142
6.3.3 Olive Ridley, Lepidochelys olivácea................................................ 144
6.3.4 FlaXback Turtle, Natato?^ depressus .................................................144
6.3.5 Green Turtle, Chelonia m ydas......................................................... 144
6.3.6 Hawksbill, Eretmochelys imbricata.................................................. 145
6.3.7 Leatherback, Dermochelys coriácea................................................ 145
6.4 Later Juvenile DevelopmentalHabitats..........................................................146
6.4.1 Loggerhead........................................................................................ 146
6.4.2 Kemp’s Ridley.................................................................................. 148
6.4.3 Olive Ridley...................................................................................... 150
6.4.4 Flatback Turtle.................................................................................. 150
6.4.5 Green Turtle...................................................................................... 150
6.4.6 Hawksbill.......................................................................................... 152
6.4.7 Leatherback....................................................................................... 152
6.5 Conclusions................................................................................................... 153
Acknowledgment.................................................................................................. 154
References..............................................................................................................155
6.1 INTRODUCTION
Sea turtles are basically creatures that spend their entire lives in marine or estuarine
habitats. Their only remaining reptilian ties to terrestrial habitats are for nesting and
restricted cases of basking. Consequently, physiological, anatomical, and behavioral
adaptations have evolved largely in response to selection in the aquatic environment.
0-8493-8422-2/97/$0.00+$.50
© 1997 by CRC Press, Inc. 137
138 The Biology of Sea Turtles
and sea turtles share many common elements with larger fishes and cetaceans in
their habitat utilization and migrations. A generalized habitat model may be con
structed for sea turtles based on ontogenetic stages (Figure 6.1):
All sea turtles move immediately to the sea after hatching, usually after dark,
and swim actively offshore. Most then undertake a mostly passive, denatant (sensu
Jones)^ migration drifting pelagically in oceanic gyre systems. Subsequently, after
a period of years, these now larger and older juveniles actively recruit to demersal
neritic developmental habitats in the tropical and temperate zones. Demersal juve
niles in some temperate zone populations make seasonal migrations to foraging areas
at higher latitudes in summer and lower latitudes in winter (see below) while those
in tropical areas are more localized in their movements. When approaching maturity,
pubescent turtles move into adult foraging habitats. In some populations adult
habitats are geographically distinct from juvenile developmental habitats;^ "^in others
they may overlap or coincide.'’’^ Upon maturity as the nesting season approaches
adults make a contranatant migration toward the nesting beaches. Most mating occurs
Habitat Utilization and Migration in Juvenile Sea Turtles 139
at poorly defined courtship areas that are close to the nesting beaches relative to the
distant foraging areas. After mating the females move to their respective nesting
beaches.^’^ Courtship areas may be directly off the nesting beaches,^ or remote from
the beaches,depending on the population. During the nesting season, females
usually become resident in the internesting habitat in the vicinity of the nesting
beach. The focus of the present paper is habitat utilization and migration of juvenile
sea turtles and nursery and developmental habitats.
6.2 METHODS
Although some authors have attempted to define objective size catagories of sea
turtles by ontogenetic stages,temiinology for juvenile life history stages has been
varied and often imprecise. The words hatchling and neonate have been widely used
and clearly refer to animals that have quite recently hatched. The term post-hatchling
obviously refers to individuals that are larger and older than a hatchling, but the size
(or age) at which a hatchling becomes a post-hatchling varies by author and is ill
defined. Even less well defined is the size or age when a post-hatchling becomes a
juvenile or an “immature” or a “yearling”, and when this stage becomes the subadult
stage. Many authors have used the teim subadult to refer to all juvenile turtles that
have recruited to demersal, neritic habitats. This term is a misnomer for animals that
may not mature for 15 to 45 years. Critical ontogenetic habitat shifts such as that
from the pelagic nursery to the demersal developmental habitats occur in different
species or even populations at different ages and sizes. For instance, Eretmochelys
imbricata and Lepidochelys kempi usually make this shift at a smaller size (and
younger age) than Chelonia mydas, which in turn makes this shift at a smaller size
(and younger age), than does Caretta caretta (see below). Even within C. caretta,
western North Atlantic populations make the shift from the juvenile pelagic habitat
to demersal habitat at a smaller size (and younger age) than do western South Pacific
populations (see below). The adult stage would seem to be easily defined (i.e.,
minimum size of nesting females and clearly mature males with large dimorphic
tails); however, large numbers of Chelonia mydas, Caretta caretta, and E. imbricata
that are larger than the minimum breeding size of the nesting females recorded on
the beaches are still sexually immature and hence incapable of br ee di ng. Se xual l y
immature turtles that are within the size of breeding turtles also occur with L. kempii
and Dermochelys coriacea}"^ The average female commences to breed at a size only
slightly smaller than the average size of the entire nesting population.C onse
quently, the size and age range for transition from the juvenile to the adult stage is
usually broad within most populations. In order to avoid further confusion concern
ing ontogenetic stages, we have restricted our use to the terms hatchling (or neonate),
juvenile, and adult. Hatchlings refer to sea turtles that have quite recently hatched
and are still on the nesting beach or at sea only until they commence to feed (i.e.,
while they are dependent on the internalized yolk sac).^^ The at-sea period for the
hatchling is short (days), so that size is still essentially similar to that at hatching.
Juveniles include turtles that have commenced feeding, but have not attained sexual
maturity. The term adult will be used to describe all turtles that have attained adult
140 The Biology of Sea Turtles
size, i.e., that are larger than minimum breeding size. Adult-sized turtles within
feeding areas will include a mixture of sexually immature and mature animals, unless
maturity is assessed via direct observation of the gonads and associated ducts or
from a past breeding history. We will reference other terms when used by other
authors. The size of sea turtles is usually given as curved carapace length (ccl, length
measured with tape over the curvature of the back) or straight carapace length (scl,
straight line length of the carapace measured with calipers). When the type of
measurement has not been designated in the literature we have referenced it as
undesignated length (ul).
The early juvenile nursery habitat of the loggerhead has been best documented of
all sea turtle species. The occurrence of small loggerheads far at sea often in close
association with drifting Sargassum within the North Atlantic Ocean has been known
for several y e a r s . S e v e r a l authors have noted the potential importance of oceanic
convergence zones and major gyre systems in the distribution and local concentration
of juvenile loggerheads.^^ Recent studies have provided strong support for these
hypotheses. Witherington^^ has documented substantial numbers of neonate logger-
heads along the western edge of the Gulf Stream in convergence zones actively
feeding among Sargassum. Similarly, Richardson and McGilivary^^ recorded post
hatchling loggerheads actively feeding (on aeolian transported insects) in Sargassum.
Behavioral studies in the laboratory^^’^^ have shown that neonate loggerheads are
attracted to floating seaweed and hide in the weed motionless for long periods of
time. (The hatchlings’ basic brown and tan coloration renders them cryptic in the
Sargassum habitat.) Carr et al.^^ hypothesized that neonate loggerheads from the
southeastern U.S. swam offshore and entered the Florida current (subsequently the
Gulf Stream) where they became entrained and were passively transported across
the North Atlantic to the eastern Atlantic. Then they drifted south in the North
Atlantic gyre past the Azores and the Canary Islands and subsequently returned with
the North Equatorial CuiTent back into the western Atlantic (Figure 6.2). He based
this hypothesis on the small size classes (<40 cm scl) of loggerheads recorded
stranded in the eastern Atlantic.^^ (These “lost year” size classes are rare in the
western Atlantic). Witham^^ had estimated that a young sea turtle could make this
trip in about a year, but other evidence suggests it lasts longer. Several recent pa-
pers3o-33 shown that young loggerheads successfully make trans-Atlantic cross-
n:
O
g-)
DJ
D0
01
3Q .
FIGURE 6.2 North Atlantic gyre showing potential oceanic transport routes for pelagic juvenile sea turtles. (After Carr, 1987.)
142 The Biology of Sea Turtles
ings. Juvenile loggerheads have been reported to be common and often captured
incidentally in longline fisheries around the Azores and Madeira.^^-^'^'^^ Lohmann et
al."^^ (see Chapter 5) showed that juvenile loggerheads could use the magnetic field
of the earth for navigation, and could make midcourse connections to maintain their
position in the North Atlantic gyre. Studies near the Azores suggest that juvenile
loggerheads appear to spend a much longer time in the oceanic realm than previously
supposed, the “lost year” more likely representing a “lost decade”.^^ This assertion
is supported by skeletochronological studies of pelagic juvenile loggerheads captured
incidentally in oceanic driftnets in the North Pacific.^^ These turtles were 2 to 8
years old and probably originated from nesting beaches in Japan. In addition, a large
pelagic feeding aggregation of juvenile loggerheads (20 to 30 cm ul) has been
documented off Baja California.Genetic studies have shown that these animals
originated in Japanese and Australian rookeries.^^ Within the Indian Ocean, Hughes"^^
has successfully plotted the dispersal of “tagged” juvenile C. caretta from the South
African (Natal) nesting beaches by the Agulhas Current into the Indian Ocean gyre.
Thus, much evidence has accumulated that loggerhead turtle populations nesting in
the northwest Atlantic, West Indian, and West Pacific oceans utilize oceanic, pelagic
nursery grounds, and migrate with the predominant ocean gyres from west to east
for several years before returning to their western neritic foraging and nesting
habitats. The duration of the pelagic stage is highly variable and probably ranges
from three to at least ten years or longer, depending on the individual and ocean
basin. Loggerheads as small as 25 to 35 cm (ccl) have been recorded stranded in
the Chesapeake Bay (U.S.) developmental habitat.^ These animals were probably
about two to four years of age."^^ In addition, Ke i na t hus i ng satellite telemetry,
found that head-started loggerheads released at two years of age off Virginia still
exhibited the same epipelagic surfacing behavior as neonates and migrated east to
the Gulf Stream. However, a head-started loggerhead released at three years of age
became demersal and followed the same autumn migration pattern to the south as
that followed by wild demersal juveniles from Chesapeake Bay. Thus, the ontoge
netic behavioral shift from epipelagic to demersal had taken place in this individual.
Neonate Kemp’s ridleys disappear into the sea and do not reappear in the neritic
zone until they have reached a length of about 20 to 25 cm (scl)'^'^ or at about an
age of two years."^^ As their somber coloration would predict, juvenile Kemp’s ridleys
have also been reported in floating mats of vegetation.^^ Kemp’s ridleys only nest
on one restricted beach area (Rancho Nuevo) in Tamaulipas, Mexico. Thus, pelagic,
oceanic transport of neonates must be initially controlled by the hydrography in the
western Gulf of Mexico.'^^'^^ After initial entrainment in the anticyclonic Mexican
Current off the nesting beach, pelagic L. kempi should be swept into the northern
Gulf of Mexico, then eastward. Some juveniles are probably retained in the northern
Gulf until they migrate inshore and become demersal. Others may be swept south
into the Loop current, and then into the Florida Current and north into the Gulf
Stream"^^ (Figure 6.3). Most of these have grown sufficiently large to migrate inshore
and to adopt a demersal life-style by the time they reach the latitude of Long Island,
100*" 98° zcu
cr
30°N N
OJ
o'
o
D
Cl
<
O
Zi
FIGURE 6.3 Major hydrographic features in the Gulf of Mexico that may effect transport of pelagic juvenile Kemp’s ridley’s. (After Collard 1990.) w
144 The Biology of Sea Turtles
NY, or southern New E n g l a n d . O t h e r s are carried by the Gulf Stream and North
Atlantic gyre into the eastern Atlantic^"^’^"^ (see review in Marquéz).^^ The fate of
these pelagic juveniles expatriated from the Gulf of Mexico has been conjectural,
some authors suggesting that they are lost to the breeding population.^^ "^^Conversely,
there is no reason to assume that Kemp’s ridleys do not have navigational abilities
equal to those of loggerheads and, therefore, probably are able to find their way
back to the Gulf of Mexico. Mounting evidence on the behavior of demersal juveniles
in neritic developmental habitats strongly supports the latter hypothesis (see below).
In addition, Meylan^^ reported that from 1980 to 1985, 29% of stranded ridleys
(mostly juveniles) were reported from the Atlantic coast of the U.S.; the remainder
from the Gulf of Mexico. If such a large proportion of the population regularly uses
Atlantic coast developmental habitats, it is likely that they can return to the Gulf of
,^léxico to breed.
Neonate olive ridleys emerge from the nest at night and rapidly crawl across the
beach to enter the surf.'^^’^^ Nothing is known of the early juvenile nursery area,
although it most probably is pelagic and oceanic. Even the adults of some populations
appear to use pelagic, oceanic feeding areas when not breeding or nesting.
Pitman^® reported that 26 of 247 olive ridleys sighted at sea by shipboard observers
in the eastern Pacific were associated with floating Sargas sum. Unfortunately, he
did not note the size of these animals.
Flatback turtles emerge from the nest and rapidly enter the sea at night.^^ Evidently
they lack an oceanic stage^^’^^ and use neritic nursery areas while still feeding near
the top of the water column. Hatchling flatbacks are larger (5.7 to 6.2 cm scl) than
most other cheloniids^"^ and thus may have higher survivorship in the neritic habitat.
Hatchling green turtles enter the sea and continue to swim actively offshore for at
least 24 Thereafter, the hatchlings apparently rest at night, but continue to
swim actively offshore during the day.^^’^^’^^ Carr^^ documented several pelagic,
oceanic records of Chelonia neonates or post-hatchlings (<20 cm scl) both in the
Atlantic and Pacific.^^ Many of these were in the vicinity of Sargassum driftlines.^^
However, the occurrence of juvenile Chelonia in or near Sargassum might be for
tuitous because both the animals and the weed could be brought together passively
in convergence z one s . The strong counter-coloration of neonate green sea turtles
suggests they are open-water animals, and experiments in the l a bor a t or ys howe d
that hatchling Chelonia avoided floating weed and spent a greater amount of time
swimming in open water than did Caretta or Eretmochelys. Carr and Meylan^^ have
speculated on the most probable routes of transport for early juvenile Chelonia
hatched at Toituguero, Costa Rica, based on major oceanographic circulation patterns
in the Caribbean. Witham^^ provided a model for the transport of neonate green
Habitat Utilization and Migration in Juvenile Sea Turtles 145
turtles from the east coast of Florida in the Gulf Stream, around the North Atlantic
Basin in the North Atlantic gyre, with return to the Caribbean and Florida in the
North Equatorial Current (the same model presented for loggerheads above) (Figure
6.2). He also provided tagging records of yearling green turtles released in Florida
and recovered in the Azores and Madeira. Oceanic records of juvenile Chelonia are
not as common as those for Caretta. In addition, the recruitment of Chelonia to
neritic developmental habitats in general occurs at smaller sizes (=30 to 40 cm
cci) 13,71-74 those for Caretta (=50 to 70 cm ccl).^’^ Therefore, Chelonia must
spend a shorter time in the oceanic nursery and recruits to demersal developmental
habitats at a younger age than does Caretta, or it has a much slower pelagic growth
rate.
6.3.6 H awksbill, E r e t m o c h e l y s ¡m b r ic a t a
Upon hatching, neonate hawksbill behavior is much like that reported for other sea
turtles with emergence at night and immediate movement to the sea.^^ Carr^^ provided
several pelagic records of early juvenile hawksbills (5 to 21 cm scl) many of which
were found in association with Sargassum. Other recent records have been provided
by Redfoot et al.,^^ Limpus et al.,^^ and Parker.^^ Unfortunately, other than the one
original record Parker provided (=23 cm ccl), most of the others he cited were based
on beach strandings, and many of the animals might have been neritic and demersal
prior to death. At least one of his records^^ was based on a benthic hawksbill captured
in clam tongs inside Chesapeake Bay. In the laboratory, neonate hawksbills were
attracted to floating weed and used it for cover where they remained motionless for
long peri ods. Appar ent l y at least some hatchlings may remain on reefs close to
their natal beaches.^^’^®However, Boulon^^ reported that juvenile hawksbills recruit
to the demersal coral reef habitat in the Virgin Islands at 20 to 25 cm (scl). This
range complements the largest verified pelagic records (21 cm scl, 23 cm ccl) noted
above. Thus hawksbills in the Atlantic recruit to the neritic developmental habitat
at a smaller size than either the loggerhead or green turtle, probably at an age of 1
to 3 years. Age and growth have not been determined in pelagic hawksbills, and this
tentative age estimate is based on growth rates in demersal juvenile hawksbills,^^
and pelagic juvenile loggerheads^^ and juvenile Kemp’s ridleys."^^ In the Indo-Pacific
region hawksbills recruit to inhabit coral reefs at a larger size (usually >35 cm [ccl])^
and presumably at a greater age.
6.3.7 L eatherback, D e r m o c h e l y s c o r ia c e a
Hatchling leatherbacks move immediately to the sea and swim actively offshore.
Leatherbacks are pelagic even as adults, and it is not surprising that neonates are
more active than other species of sea turtles, swimming offshore both day and night
for at least six d after entering the sea.^^ The fate of pelagic juvenile leatherbacks
after leaving the nesting beach is one of the great mysteries of sea turtle biology.
Other than neonates, there are very few records of leatherbacks <110 cm
If Zug and Parham’s^^ age estimates are coiTect, and leatherbacks grow rapidly, then
juveniles virtually disappear for four years. Lutcavage and Lutz^^ noted that small
146 The Biology of Sea Turtles
leatherbacks need to consume gelatinous prey (the low-energy food upon which they
specialize) equal to their biomass every day in order to support routine metabolism.
Their food requirements must even be higher to promote rapid growth. In the oceanic
realm, production necessary to support high biomass of gelatinous (and other)
animals is found primarily in areas of major upwelling such as those along the
eastern sides of the major ocean basins or perhaps in the Equatorial Convergence
Zones. These areas may provide nursery grounds for leatherbacks.
Sf>
im
u.
FIGURE 6.4 Length frequency distribution (ccl) for loggerheads stranded in Virginia from
1974 to 1995.
50 j
I male
40 - ¡Female
30
g
20
I
10
FIGURE 6.5 Length frequency distribution (ccl) for loggerheads collected in foraging
habitats in Australia.
loggerheads he tracked from Virginia wintered in two basically distinct areas: one
group went down the coast, stayed inshore (hesitating during warm spells at various
major inlets), and wintered inshore off southern Georgia and Florida (one traveled
as far as the Florida Keys). The other group followed the same inshore route south
of Cape Flatteras to about Cape Lookout, NC, then moved offshore to winter on
reefs along the shelf edge on the western side of the Gulf Stream. One might
hypothesize that the group wintering off North Carolina was derived from Geor-
gia/South Carolina rookeries, and those that wintered off Florida were from Florida
rookeries. Only further genetic research will tell.
Juvenile loggerheads of about the same size distribution (50 to 80 cm scl) are
common during the summer in estuaries as far south as Mosquito and Indian
Lagoons, Florida^®"^’^^^ and in the Gulf of Mexico. In South Carolina and Georgia
they exhibit seasonal emigration from estuaries into the ocean in the autumn, with
immigration occurring in the s p r i n g . I n Mosquito Lagoon, Florida, juvenile
Carena occur year-round, but are captured in smallest numbers during February and
March (the time of lowest water tem perature).The reason for this may be because
the turtles emigrate out into the ocean, or rather they may be inactive, actually
brumating in the mud during this period. Juvenile loggerheads commonly brumate
in winter by digging head first into the mud in the Canaveral Ship Channel (in the
ocean not far from Mosquito L a g o o n ) . W e have never observed any evidence
of brumation in Virginia, nor have Epperly et al.^^^ in North Carolina where winter
water temperatures fall below the lethal lower limit for loggerheads (5 to 6.5°C).^
Henwood^^^ noted that juvenile loggerheads reached their peak abundance in winter
in Canaveral Ship Channel, and that some turtles tagged there in winter were
recaptured to the north in summer. In contrast, to the well-defined seasonal foraging
migrations exhibited by juvenile loggerheads along the Atlantic coast of the U.S.,
both large (>70 cm ccl) juvenile and adult Caretta were resident all year in the coral
reefs of the southern Great Barrier Reef and in Moreton Bay, Australia in the
southwestern Pacific.^’^^"^ The most southerly of these sites, Moreton Bay, has an
annual water temperature range of 16 to 28°C, much more moderate than those
found in estuarine and coastal waters north of Florida in the U.S. After recruitment
into the eastern Australian demersal habitats, these juvenile loggerheads show strong
forage site fidelity to relatively small areas, mostly remaining within a few square
kilometers for the next 8 to 20 years of growth to sexual maturity. Adult
loggerheads, after completing their first breeding migration to distant nesting
beaches, return with high fidelity to the same juvenile foraging areas in which each
had completed its juvenile life.^^"* They also return to the same foraging sites fol
lowing subsequent breeding migrations. It is strongly indicated that individual
loggerheads in the southwestern Pacific occupy very localized home ranges for the
decades that span their later juvenile and entire adult lives.
the juveniles carried by the Gulf Stream into the Atlantic probably make an
active rather than a passive migration west from the edge of the continental shelf to
find suitable shallow developmental habitats such as Long Island Sound. Although
Gulf Stream gyres may impinge upon the edge of the continental shelf, they rarely
cross the shelf to near coastal habitats.^In the Gulf of Mexico, shallow coastal
habitats serve as foraging areas for Kemp’s ridley throughout the year, although
there is evidence for seasonal offshore movements in response to low water temper
atures in winter."^^'^^^ OccuiTence of L. kempi in shallow water off northwest Florida
is seasonal, with no turtles captured in the coldest winter months. In New York
and New England, juveniles must emigrate coastally to the south or face cold
stunning and death in the winter.^^’^^’^^^
Chesapeake Bay serves as an important developmental habitat for juvenile L.
kempiP'^"^ The summer population size has been estimated to be 211 to 1083 (a
minimum estimate) with a mean size of about 40 cm (ccl)^^^ (Figure 6.6). Juvenile
L. kempi preferred shallower summer habitats in Long Island Sound (<8
and Chesapeake Bay (<5 m)^^ than were used by juvenile loggerheads,^^ and often
foraged in submerged aquatic grass beds for Callinectes sapidus and other crabs.^
The seasonal migration pattern is similar to that of the loggerhead, with immigration
in May and June and emigration from September to November. Of 57 Kemp’s ridleys
tagged in Chesapeake Bay, 2 were recaptured there in subsequent summer s Thus ,
site fidelity is possible for summer foraging locations. Upon leaving Chesapeake
Bay in autumn, juvenile L. kempi migrate down the coast, passing Cape Hatteras in
December and January. These larger juveniles are joined there by juveniles of the
same size from the North Carolina sounds and smaller juveniles from New York and
New England to form one of the densest concentrations of Kemp’s ridleys outside
the Gulf of Mexi co. Kei nat h*^^ used satellite transmitters to track three juvenile
Kemp’s ridleys released in Virginia in October. They followed the same inshore
route noted for loggerheads above, and two individuals migrated as far as Florida
by mid-January. (The transmitter failed on the third turtle in November when the
animal was approaching Cape Fear, NC). Henwood and Ogren^^^ noted that juvenile
Kemp’s ridleys reached their maximum abundance off Cape Canaveral, FL in Jan
uary to March, and that their tagging data suggested a northward movement in
summer and a southward movement in the winter. These data complement and
support the pattern of seasonal migration noted to the north.
Ogren"^^ reported that the entire northern coast of the Gulf of Mexico from Port
Aransas, TX to Cedar Key, FL served as demersal developmental habitat for juvenile
Kemp’s ridleys. Areas where significant concentrations of juvenile ridleys occurred
included: the Sabine Pass area of Texas; Caillou Bay, Terrabonne Parish, LA; and
Big Gulley east of Mobile Bay, AL. The smallest juveniles (20 to 25 cm scl) were
found in two areas: one off western Louisiana and eastern Texas, and the other off
northwest Florida.^'^’^^'^ Most juvenile L. kempi in the latter area were captured at
depths <6.1 m, and all ridleys <25 cm (scl) were from <1 m. Juvenile Kemp’s ridleys,
then, recruit to shallow estuarine and coastal habitats in the western Gulf of Mexico
and off New England and New York in summer. In winter they migrate south and/or
offshore to avoid cold temperatures, but may remain resident in some areas of the
150 The Biology of Sea Turtles
45 T
iZ 15 -
length (cm)
FIGURE 6.6 Length frequency distribution (cel) for Kemp’s ridley stranded in Virginia
from 1979 to 1995.
Gulf of Mexico that are sufficiently warm. It is possible that some individuals may
brumate at temperatures
40
Hi adult
»pubescent
30 Oprepubescent
è
zUJ
3 20
o
LU
DC
Ll
10
0 10 20 30 40 50 60 70 80 90
IL
100 110 120 130
FIGURE 6.7 Length frequency distribution (ccl) for green turtles captured in Shoalwater-
Bay, Australia.
became larger (28.8 to 66.8 cm scl) they moved into the Indian River Lagoon seagrass
beds to forage. Henwood and Ogren^^^ also noted that juvenile green turtles off Cape
Canaveral were smaller than those in the Mosquito Lagoon estuary.H enw ood and
Ogren^^^ suggested that these small coastal green turtles might be in early develop
mental stages prior to the shift to herbivory and recruitment to shallow inshore
feeding pastures. Mendonca and Ehrhart^®"^ showed that juvenile green turtles
recruited to the Mosquito Lagoon (Florida) estuarine developmental habitat at 30 to
40 cm scl and that some individuals became resident, showing foraging site fidelity.
Many turtles remained in the Lagoon year-round until they approached maturity.
They also noted that the catch rates of juvenile Chelonia were highest in the summer,
but that the winter decline might be due in part to turtle inactivity (brumation?).
Severe cold-stunning events in winter in 1977 and 1978 showed that green turtles
were still common in the Lagoon. Felger et al.^^^ reported Chelonia to burrow in
the mud (brumate) in winter in the Gulf of California. Mendonca*^^ noted that
juvenile green turtles in Mosquito Lagoon wandered as far as 5 to 10 km a day
when the water was cooler (11 to 18°C) but established home ranges of 1.2 to 4.1
km when temperatures were above 25°C. Bjorndal and Bolton^^^ reported that tidal
embayments were important developmental habitats for juvenile green turtles in the
Bahamas. Their tagging studies showed that immigration occurred to other areas of
the Bahamas, Colombia, Cuba, Dominican Republic, Haiti, Nicaragua, Panama, and
Venezuela.
Balasz^"^ and G r e e n n o t e d that juvenile green turtles in the Pacific remained
on reef habitats and consumed macroalgae. Balasz^"^ also reported that almost all
juvenile green turtles he tagged in the Hawaiian Islands were recaptured in the same
resident areas. Similarly, all the juvenile green turtles tagged by G r e e n i n the
Galapagos were recaptured within the archipelago. In the southwest Pacific juvenile
greens occupy the same wide range of habitats as the adults, being common on coral
reefs where algae is the dominant food^ and in inshore embayments where the main
152 The Biology of Sea Turtles
food items are seagrasses.^^ These juvenile greens have displayed strong feeding site
fidelity within their feeding areas (tens of square kilometers) over many yearsT^’^^^^
Some juvenile greens have been under observation in the same localized feeding
areas in the southern Great Barrier Reef for two decadesd"^ Juvenile green turtles
can be resident for years in tropical or subtropical developmental habitats with stable
or moderately stable seasonal temperatures. They may utilize continental foraging
areas in temperate latitudes (to about 40°N) in summer, but must return to subtropical
latitudes in winter to avoid cold stunning. Brumation may occur during colder
periods in subtropical estuaries.
6.4.6 H awksbill
Hawksbills are restricted to the tropics more than any other sea turtle. Juveniles
recruit to developmental habitats at a very small size (20 to 25 cm scl) or even as
hatchlings (see above). The developmental habitat includes shallow (<20 m) coral
reefs and mangrove estuaries^^ where there are abundant sponges, the principal food
of this s p e c i e s . Th e developmental habitat does not seem to differ from that of
the adult, and juveniles and adults are taken together in the same foraging areas.'^’^^'^
Boulon^^^ tagged juvenile hawksbills in the Virgin Islands and found that many
migrated to areas outside the Virgin Islands. (Nesting hawksbills from the Virgin
Islands have also been found to be mostly mi g r a t or y) . I n contrast, in eastern
Australia, juvenile hawksbills >35 cm (ccl) have displayed a high fidelity to localized
feeding areas in coral reef habitats with some individuals being associated with the
same site for over a decade."^ In addition, juvenile hawksbills less than 30 cm ccl
are uncommon in the Great Barrier Reef waters, and none have been recorded in
demersal developmental habitats.^^ Very little research has been conducted on behav
ior and habitat utilization in juvenile hawksbills.
6.4.7 L eatherback
Although leatherbacks are pelagic, they recruit seasonally to temperate and boreal
coastal habitats to feed on concentrations of jellyfish.^ In the western Atlantic,
juvenile Dermochelys appear in these habitats at about 110 to 120 cm (ccl), based
on stranding data^^’^"^®(Figure 6.8). If we accept the size at maturity (based on the
nesting population in St. Croix) as 137 cm (ccl) ( the size of the smallest nesting
female) or as 157 cm (ccl), (the approximate average size of nesting females),
then a substantial percentage of leatherbacks stranding along the U.S. east coast
must be large juveniles. Aerial surveys have shown large numbers of leatherbacks
off the northeast coast of Florida in February,southern Virginia in late April,
and the Gulf of Maine in summer.Leatherback standings from 1980 to 1991 in
Florida occurred mostly between October and April.^^ Sightings of leatherbacks off
Massachusetts peak in August, with no live sightings before June or after October.
Although several tagging and tracking studies have been done on adult females that
show long-distance oceanic migration to higher-latitude coastal feeding habitats,
virtually no work of this kind has been done on juveniles. Paladino et al.^“^^ pointed
out that leatherbacks were inertial endotherms, but that the high surface area-to-
Habitat Utilization and Migration in Juvenile Sea Turtles 153
9O
4im
»
11
1 ^ ^ 1
-------- --------- r
1
T--------
1
1
1
T--------T--------- — —
CTk os OS OS as CJS CiS
CIS OS OS OS OS os OS CJS
CM ro in
T-
vD OO OS o
CM
•t—
CM
CM
CM
o o o o o
in o o o
00 o o o o
^ CM ro MD r-
T-.
OS o ■»—- CM
CM CM CM
Length (cm )
FIGURE 6.8 Length frequency distribution (ccl) for leatherbacks stranded in Virginia from
1979 to 1995.
mass ratio in juveniles precluded body temperatures significantly higher than ambi
ent. The size range at which leatherbacks become efficient endotherms is probably
the same as that when they first appear in coastal, boreal waters (110 to 120 cm ccl).
The leatherback shares many attributes with the endotheimic scombroid hshes
(tunas). Both reproduce in the tropics. Both have been able to expand their effective
foraging ranges into higher latitudes and greater depths (into the thermocline), well
beyond the normal range of their nonendothermic relatives.
6.5 CONCLUSIONS
A comparison of the ontogenetic patterns of habitat utilization and migration among
the seven species of sea turtles leads to the following hypotheses: natural selection
will favor those nesting locations with the highest survivorship during the vulnerable
early juvenile stage when predation is highest. Given all sea turtles nest in tropical,
subtropical, or warni temperate latitudes, the most important determinant of nesting
beach locations probably is survivorship of hatchlings and small juveniles in the
nursery habitats.
In sea turtles as in fishes, the numbers and diversity of potential predators and
resulting mortality rates are inversely proportional to the size of the juvenile. The
advantage of pelagic, oceanic nurseries is the low density of predatory fishes and
sea birds there dictated by low primary production.‘^^’^‘^'^ (Productive-upwelling areas
are exceptions.) Based on predator mouth size alone, few potential avian or fish
predators, except s h a r k s , a r e capable of engulfing hard-bodied epipelagic prey
(such as sea turtles) >20 to 30 cm in diameter. Marine crocodiles, which can also
be significant predators of most size ranges of marine turtles, are largely restricted
to tropical inshore margins of marine turtle foraging habitats. Marine mammals
154 The Biology of Sea Turtles
appear to be rarely predators of marine turtles. Hawksbills and Kemp’s ridleys recruit
from pelagic, oceanic nurseries to neritic, demersal developmental habitats at about
20 to 25 cm (scl); green turtles do so at about 30 cm. In order to avoid predation
from sharks and large teleosts, the smallest demersal recruits tend to use structured
habitats such as reefs that provide cover (hawksbills and green turtles) or very
shallow habitats where larger predators cannot go (Kemp’s ridleys). Juvenile log
gerheads stay in the pelagic, oceanic nursery much longer and to a much larger size.
Apparently, so do some populations of olive ridleys, which may use oceanic feeding
grounds as adults (mostly in up welling areas with abundant crustacean prey). Log
gerheads are opportunistic carnivores utilizing a wide variety of prey, including
molluscs, crustaceans, horseshoe crabs, and gelatinous m acroplankton.They seem
to be well adapted for long periods of opportunistic foraging (mostly on gelatinous
prey) in the oceanic realm, although they have relatively slow growth rates there.^^
Older juvenile and adult green, hawksbill, and Kemp’s ridley turtles have specialized
diets (herbivory, spongivory, and cancrivory, respectively), and might be compelled
by dietary energetic constraints to seek out more productive demersal developmental
habitats at smaller sizes than loggerheads. In addition, Kemp’s ridley, the smallest
species of sea turtle, seems to have a shorter life cycle and matures at an earlier age
than other species of the Cheloniidae."^^
Most species that primarily utilize pelagic, oceanic nurseries and have relatively
long (>3-year) oceanic stages tend to have high-density nesting localities located
adjacent to major oceanic currents that can maintain small juveniles passively and
safely for sufficient time for them to grow large enough to lower predation risk and
to have the mobility to actively swim to developmental habitats. Loggerheads, green
turtles, and both Kemp’s and olive ridleys seem to fit this pattern. Nesting is mostly
restricted to certain sites used (or historically used) by large numbers of nesters.
Small scattered nesting sites along the fringes or outside of these primary sites are
probably constrained by very low survivorship of the pelagic juveniles caused by
poor access to the passive hydrographic pathways that lead to the oceanic nurseries.
Hawksbills do not fit this pattern well. Although some concentrated nesting areas
exist^^® or existed historically, solitary nesting is widespread and frequent mostly on
islands.Hawksbills may have the shortest pelagic stage of all sea turtles (except
the flatback), or hatchlings may recruit directly to demersal developmental habitats
on coral reefs or mangrove flats. Also, they tend to occur in archipelagos where
local or regional hydrographic features may be more important than major oceanic
gyres during the short passive transport stage of the juveniles. Leatherbacks are
pelagic during all stages of their life, and utilize oceanic habitats more than any
other sea turtle.^^’^^* Although there are some high-density nesting a r e a s , m u c h
scattered nesting occurs, and this species may have lower re nesting fidelity than
other sea turtles. This apparent enigma may be explained by the larger size and
faster growth rates and superior swimming ability of neonate leatherbacks. These
attributes may render juvenile leatherbacks much less dependent on passive transport
to the oceanic nurseries. Young leatherbacks undoubtedly use major oceanic current
systems for transport, but may be more capable of actively reaching those passive
pathways.
Habitat Utilization and Migration in Juvenile Sea Turtles 155
ACKNOWLEDGMENT
Virginia Institute of Marine Sciences contribution # 2026.
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V Sea Turtle Locomotion:
Mechanisms, Behavior,
and Energetics
Jeanette Wyneken
CO NTENTS
0-8493-8422-2/97/$0.00+$ 50
© 1997 by CRC Press, Inc. 165
166 The Biology of Sea Turtles
7.1 O V E R V IE W
Marine turtles depart functionally and moiphologically from other chelonian lin
eages. Their form of locomotion and of their locomotor adaptations, perhaps more
than any other features, make sea turtles unique from other turtles. Very large adult
size, hypertrophied phalanges, distinctive locomotor patterns, relatively high activity
levels, and their nearly totally aquatic life-style are all deviations from the historical
emphasis of this morphologically conservative vertebrate group. Consequently, sea
turtles have attracted the attention of many authors who described, listed, or inves
tigated the adaptations that sea turtles possess for their marine life-style.
In general, the locomotion of turtles has unique features when compared with
that of other vertebrates. Because of the rigid carapace, all propulsion must come
from the limbs. While all terrestrial turtles and freshwater turtles (with the exception
of Carettochelys) utilize diagonally alternate limb movements to swim or crawl,^'^
marine turtles use both forelimbs, simultaneously swept through the water during
swimming.^’^®Though this swimming motion is technically described as an asym
metrical gait (because the forelimbs move simultaneously as a pair rather than a
forelimb and hindlimb acting as a pair; see Figure 7.1), the term is counterintuitive
when describing sea turtle locomotion. So for this discussion I will abandon typical
“gait jargon” and instead use terminology prevalent in the behavioral literature. Thus,
the most common locomotor pattern used by sea turtles is the “powerstroke,”^* ’^ in
which the forelimbs are swept through the water simultaneously, like flapping wings.
When covering distances beyond several body lengths, all sea turtles utilize the
powerstroke to produce thrust. During their post-hatching ontogeny, cheloniid sea
turtles employ a “dogpaddling” stroke (symmetrical gait) in which diagonally oppo
site limbs move together to produce thrust.^®’^^ Similarly, following offshore migra
tion, hatchlings and post-hatchlings primarily rely upon the a “rear flipper kick”
while they reside in the pelagic environment. In contrast, dermochelids
utilize the powerstroke almost exclusively and do not undergo similar ontogenetic
changes in locomotion.
Three major themes dominate any consideration of sea turtle locomotion. First,
cheloniids undergo ontogenetic changes in their predominant form of thrust produc
tion. These changes are closely linked to behavioral and ecological shifts. Under
most circumstances, the forelimbs have primary responsibility for thrust production.
Yet, all 4 limbs work to generate thrust during some phases of life in young sea
turtles. Second, during terrestrial locomotion, all four limbs are used to generate
Sea Turtle Locomotion: Mechanisms, Behavior, and Energetics 167
Symmetrical Gait
Diagonally opposite limbs move together
Asymmetrical Gait
Contralateral limbs move together
FIGURE 7.1 Diagrammatic representation of turtle gaits. Gaits are defined as symmetrical
or asymmetrical by both the duration of foot contact with the ground (or other foot action,
as in swimming) and by the phase relationship between the fore- and hindfeet.'^ These
designations are cumbersome when applied to sea turtle locomotion. Symmetrical gaits (upper
diagram) are typical of the locomotion by most nonmarine turtles as well as marine turtle
dogpaddling and crawling by Caretta, Eretmochelys, Lepidochelys, and hatchling Chelonia
and Natator. The powerstroke is an asymmetrical gait. Asymmetrical gaits (lower diagram)
are also used by adult Chelonia and Dermochelys during crawling.
thrust in all turtle species. However, the details of sea turtle terrestrial locomotion
make it distinct. Third, migratory behavior dominates sea turtle life. Morphological,
behavioral, and physiological adaptations are clearly related to efficient, long-dis
tance movement through the water (particularly during migration).
FIGURE 7.2 Crawling by a Caretta hatchling is typical of the locomotion of all other
cheloniid hatchlings. Diagonally opposite limbs move together. Tracings were made from
ciné recordings filmed at 32 fps. Elapsed time (t) is given in seconds to the left of each tracing.
the end of retraction, the hind flippers are abducted and dragged as the forelimbs
complete their retraction. Unlike other turtles, the hatchling leatherback gait
includes a period when all four flippers are in the air simultaneously while the
ventrum supports the body. When D. coriacea is placed on a smooth surface it can
switch to use diagonally opposite limbs in an alternating pattern. This gait was
observed only under experimental conditions.
Sea turtle crawling differs kinematically from mechanisms used by other turtle
species. The “crutching” movements seen in adult C. mydas, N. depressa, and D,
coriacea are unique within the Testudines. Adults of other cheloniid species employ
quadrupedal gaits in which diagonally opposite feet move as a pair. As marine turtles
approach adulthood and their mass increases, they support themselves on the carpus
and anterior edge of the manus rather than on the palmar surface.
7.2.2.1 Chelonia
Adult female green turtles typically come ashore only to nest, while males remain
permanently aquatic. However, at isolated locals, such as the remote northwestern
Hawaiian Islands (U.S.), green turtles of both sexes (adults as well as immature
turtles) crawl ashore to bask on oceanic beaches.^® Along coastal North Carolina,
juvenile C. mydas and L. kempi have been observed crawling on land,^°' presumably
to bask.
170 The Biology of Sea Turtles
All adult and subadult Caretta, Eretmochelys, and Lepidochelys move diagonal pairs
of limbs to produce thrust. This pattern is often described as a “typical” quadrupedal
or tetrapod gait, though it differs by lacking any axial undulations and both the
manus and the crus support the weight of the body.
7.2.2.3 Natator
Adult flatback turtles appear to rely on both methods of terrestrial locomotion,
depending upon circumstances. Bustard^ reports that this species uses simultaneous
movements of all 4 limbs when ascending the beach, but switches to thrust with
diagonal limbs moving together when crawling up a dune face.
7.2.2.4 Dermochelys
Only adult female leatherback turtles come ashore and only when ready to nest.
Detailed studies of terrestrial locomotion rely upon direct observation and films of
nesting emergences, and upon films of a juvenile raised in captivity.'^ Adult and
juvenile leatherback turtles use crutching movements of the forelimbs, with simul
taneous pushing by the hind limbs. This “swing and stance” gait of the adults and
juveniles is very similar to the locomotor pattern used by hatchlings (Figure 7.3).
However, leatherback turtles are massive and do not lift the body far, if at all, from
the sand.^^ The adult gait involves support of the weight of the animal on the plastron
while all 4 flippers are protracted in the air; the turtle then moves forward in a series
of lunges (Figure 7.4). A single captive-raised juvenile, crawling under experimental
conditions, occasionally used its hind limbs in an alternating fashion for a few steps.
In nature, juveniles are not known to crawl ashore.
Nesting leatherback turtles leave a sinusoidal track when crawling ashore.
Pritchard^^ suggests that twisting in the track is due to the efforts of the animal to
minimize the angle it has to climb “uphill” to nest. Another possible explanation is
that since the rear flippers are occasionally used alternately, the direction of push
(the thrust vector) from the rear could torque the body of the animal around as it
moves forward.
Sea Turtle Locomotion: Mechanisms, Behavior, and Energetics 171
7.3 A Q U A T IC L O C O M O T IO N
Structural and adaptive diversity exist among marine turtle species along with con
siderable conservatism. Specifically, the form of the body and limbs is modified
from the basic turtle body design. Marine turtle body form is characterized by
reduction in the axial skeletal elements and increased fontanels in the carapace and
plast ron, a heavy head with a nonretractable neck,^^’^"^ and minimal cervical, axial,
and inguinal pouches. The pectoral limbs are modified into wing-like fore flippers
and the hind flippers have become paddle-like or rudder-like. Functionally, sea turtles
have exploited a mechanical system for propulsion that is not utilized by other turtle
groups. For most of their swimming activities, cheloniid and dermochelid sea turtles
use a largely lift-based mechanism of producing t h r u s t . T h e flippers
serve as wings (or propellers) as well as paddles. Two drag-based mechanisms,
“dogpaddling” and “rear flipper kicking,” are also in the repertoire of hatchling and
juvenile cheloniid locomotor patterns. However, these are reserved for activities
other than covering long distances quickly and efficiently.
172 The Biology of Sea Turtles
The body fonri of sea turtles is highly streamlined when compared with semi-
aquatic and freshwater turtles.Streamlining contributes to efficiency while swim
ming by minimizing drag (the resistance to moving through the water).
Elongate flippers are typieal of secondarily aquatic vertebrates that propel themselves
with paired appendages (e.g., seals, penguins, marine turtles, and plesiosaurs).
The modification of the pectoral appendage from a limb with a typieal foot (Figure
7.5A) to an elongated, semirigid flipper Figure 7.5B) is, perhaps, the most striking
specialization of sea turtles. The radius and ulna are functionally fused by thick
connective tissue. The digits are elongated and made stiff by layers of fibrous
connective tissue overlaid by a scaled skin. The procoracoid of the pectoral apparatus,
located deep to the pectoralis and supracoracoideus muscles, is greatly expanded to
accommodate the proportionately greater muscle mass which retracts, abducts, and
rotates the flipper (Figure 7.5C, D).
The rear flippers are neither elongated nor particularly stiff. The interdigital
musele and connective tissue, as well as the overlying connective tissue, make the
hind flipper appear to be a paddle, though it functions both as a paddle in thrust
productions^ and as a rudder or elevator in steering.^^
Walker^^^ made qualitative comparisons between the limb skeletal components
of marine and aquatic turtles. The musculoskeletal system has been described in
detail by several investigators.^^’^s-34 orientation of the skeletal elements in the
fore- and hindlimbs of a crawling leatherback turtle was described by extrapolation
from radiographs of posed preserved limbs.
Sea turtles swim vigorously underwater using their forelimbs to provide thrust. The
synchronous sweeping of the flippers causes the axial body to rock so that it cycles
through positive and negative angles of attack, (but does not reach the point of stall).
During normal swimming all species avoid stall.
Sea turtle hat chl ingsand juveniles^^ differ from other marine migrants by
operating at moderate Reynolds numbers (Re = 1.1 to 4.6 x 10"^).^^ The Re for
hatchlings and juveniles are similar to those of small salmonids'^® but lower than
those recorded for many other aquatic migrants (e.g., tuna,^^ 3 x 10^; salmonid fish,"^®
2.5 X 10^ to 5.2 X 10^; penguins,^^’'^^ 1.26 to 1.76 x 10^; dolphins:'^^ 6.5 x 10^;
p o rp o is e s ,1.3 x 10^; whales,^^ 3 x 10^; and sea lions,'^'^ 2.03 to 2.87 x 10^). All
else being equal, similar Re imply similar flow conditions (and comparable relation
ships of inertial and viscous forces).
2 12
3
13
14
15
16
9 19
10 20
I
FIGURE 7.6 Powerstroking by a Chelonia mydas hatchling. Dark vertical bars to the left
of the tracings indicate where the forelimbs are undergoing simultaneous protraction. Open
vertical bars to the left of the tracings indicate that the forelimbs are undergoing simultaneous
retraction. The swimming turtle was filmed at 58 fps. Each tracing comes from consecutive
frames that are 0.017 s apart. Lift-based propulsion, at the end of protraction and start of
retraction, creates the greatest speeds. (Redrawn from Wyneken, J., Comparative and Func
tional Considerations of Locomotion in Turtles, Doctoral dissertation, University of Illinois,
Champaign-Urbana, Illinois, 1988, 151 pp.)
involving the hind flippers alone (Figure 7.11); the foreflippers (which play no role
in thrust production) are flexed and folded back over the carapace.
176 The Biology of Sea Turtles
FIGURE i n Diagrammatic explanation of lift forces that serve as forward thrust. Lift is
the force that results from pressure differences over a hydrofoil (such as a flipper). Lift is
perpendicular to the direction fluid moves over the foil and is perpendicular to drag (upper
diagram). A foil that is properly oriented relative to the flow can generate forward thrust
(lower two diagrams).
The predominant way in which sea turtles swim is by sweeping the foreflippers
roughly up and down as a pair (Figure 7.7). This motion, termed powerstroking in
most contemporary l i t e r a t u r e , h a s been assigned many other terms, some
more cumbersome than others (e.g., Parker: ‘‘submarine flight”; Carr and Ogren:^^
“the breast stroke”; Robinson:^® “subaqueous flight”; Davenport et al.:^^ “synchro
nous flapping of the foreflippers”). The powerstroke is a highly efficient gait that
produces propulsion during all phases of the stroke (upward-outward, then down-
ward-inward). During powerstroking, the hindlimbs usually do not contribute
thrust. Occasionally, some hatchlings coordinate slight simultaneous retraction of
Sea Turtle Locomotion: Mechanisms, Behavior, and Energetics 177
r " Effective
irectionof
Direction of
c
W ÊÊÊÊÊKÊÊÊÊÊÊÊÊÊÊÊÊtÊÊÎ^fo^
B
Effective Direction
^Direction of
of
Paddle Movement
Water Flow
FIGURE 7.8 Diagrammatic explanation of drag-based locomotion and drag forces that
serve as thrust. Drag-based propulsion involves orienting a paddle so that the blade is per
pendicular to the direction of paddle movement. (A) The blade of the paddle or flipper
encounters more resistance to moving through the water (drag) than the body does. (B) The
paddle blade or flipper during retraction in which it is feathered to minimize surface area.
(C) Propulsion (P) is generated on retraction. Protraction of the limb represents the recovery
(R) stroke.
and Chelonia hatchlings and found that the powerstroke consisted of both lift- and
drag-based components in series. Her data showed little forward thrust during the
first part of protraction, but at the end of protraction forward thrust was produced
as the flipper blade rotated through anteromedial extension. Forward thrust from lift
was also produced during the first half of retraction (when the blade twists and starts
its ventral sweep). During the second half of retraction, thrust was produced by the
flipper acting like a paddle (drag-based propulsion). Wyneken’s^®kinematic analyses
suggest that no thrust is produced during early protraction and the animal is carried
forward by momentum.
Sea Turtle Locomotion; Mechanisms, Behavior, and Energetics 179
t= 0 t = 0.34
t = 0.7 t = 0.45
t = 0.14 t = 0.62
t = 0.21 t = 0.67
t = 0.28
t = 0.74
FIGURE 7.11 Rear flipper kicking by a hatchling loggerhead sea turtle. Tracings show
synchronous protraction and retraction of the hind flippers. The hind limbs acts as paddles
and thrust production is drag-based. The turtle was filmed at 32 fps. Each tracing was made
at intervals of five frames.
180 The Biology of Sea Turtles
7.3.2.4 Steering
used infrequently. In hatchling and juvenile D. coriacea, the “axis” of the power-
stroke path differs from that of cheloniids and the route that the flipper follows is
opposite in direction. The flippers move upward and posteriorly, then downward
and anteriorly during normal powerstroking (Figure 7.12). It is difficult to divide the
leatherback powerstroke into neat protraction and retraction events because much
of the movement is upward and downward, with less anterior-posterior movement
than is seen in cheloniids. When swimming vigorously, the body tilts head down.
The flippers move posteriorly and downward, then anteriorly and upward relative
to the swimming path (or axis).^^
An overview comparing all locomotor characteristics of each marine turtle
species is provided in Table 7.1. Species-specific patterns are separated into temestrial
and aquatic locomotion and by ontogenetic categories in this summary. Because
little data exist for juveniles, those categories are omitted for simplicity.
P ostfrenzy P eriods
31
L 45
53
FIGURE 7.12 Powerstroking by a juvenile leatherback turtle. The “axis” of the leatherback
powerstroke path differs from that of cheloniids, and the route that the flipper follows is
opposite in direction so flippers move upward and posteriorly, then downward and anteriorly.
Numbers indicate the number of elapsed frames. From Renous, S. and Dels, V., J. Zool.
London, 230, 357-378, 1993. With permission.)
TABLE 7.1
Summary of Locomotor Patterns
Hatchling
terrestrial Hatchling Adult terrestrial Adult
Species locomotion swimming locomotion swimming
TABLE 7.2
Oxygen Consumption and Swimming Parameters for Hatchling Chelonia,
Caretta^ a n d Dermochelys*
Power-
VO2 Power- stroke Stroke
Migratory ml • g-’ • strokes bout Breath rate
Species phase min-^ per bout duration bout duration (strokes/s)
Cheionia Frenzy 0.021 ±0.001 18.4 ±2.3 8.4 ± 1.0 1.5 ±0.2 2.2 ± 0.4
Postfrenzy 0.013 ±0.001 13.5 ± 1.5 7.5 ± 0.9 2.8 ± 0.2 1.9 ±0.1
Resting 0.005 ± 0.0003
Caretta Frenzy 0.016 ± 0.001 21.8 ±2.5 10.6 ± 1.1 1.6 ±0.2 2.1 ±0.1
Postfrenzy 0.017 ±0.002 24.7 ± 3.0 11.6 ±2.0 2.2 ± 0.3 2.0 ±0.1
Resting 0.009 ± 0.0003
Derm ochelys Frenzy 0.010 ± 0.001 27.2 ± 3.0 19.0 ± 1.8 1.9 ± .0.3 1.4 ± 0.1
Postfrenzy 0.008 ± 0.001 24.7 ± 3.4 21.2 ± 3.4 2.4 ± 0.3 1.2 ±0.1
Resting 0.004 ± 0.0002
* All were active during the frenzy and postfrenzy, but inactive during resting measurements.
Means ±1 S.E. are based upon data collected every 5 min for 1 h. Individual turtles (n = 10 of each
species) that had not been fed were monitored separately as they swam in seawater or rested in a black
container. Both water and air temperatures were 24°C.
Surface times (during which hatchlings breathe) increase in duration following the
swimming frenzy (Table 7.2).
The combination of slow swimming speeds, slow flipper movements, and long
powerstroke bout durations in leatherbacks suggests a “marathon” strategy for the
migration (slow, steady, economical movements as opposed to rapid bursts of move
ment punctuated with many breathing interruptions). This contrasts sharply with the
“sprinter” strategy of hatchling green turtles: fast swimming speeds, quick flipper
movements, and short-duration powerstroke bouts.
adjacent to the head. Juvenile green turtles did not use the synchronous rear flipper
kick.
Witherington'"^ found post-hatchling loggerheads (43 to 59 mm standard straight-
line carapace length) in the open ocean to be relatively inactive. Most swimming
was accomplished using the rear flipper kick (n = 10 turtles). Only one turtle was
observed using the powerstroke. Davenport and Clough described the swimming
behavior of juvenile loggerheads (400 to 600 g) raised in the laboratory. They found
that these animals spent their time at the surface. More than 95% of their surface
swimming was carried out using the rear flipper kick. The powerstroke was rarely
used, and then only during escape or diving attempts. Since young loggerheads are
positively buoyant, they have difficulty diving until they reach larger sizes.
Ecologically juvenile cheloniids are found in the epipelagic zone (far from bottom
structures and food in many cases).
The swimming behavior of captive juvenile Pacific ridley turtles (L. oliváceo)
was described by Davenport and Pearson. These turtles, like loggerheads, are
positively buoyant and spend their time at the surface of the water. The majority of
their swimming involved synchronous or asynchronous rear flipper kicking. Unlike
loggerheads, the olive ridley turtles did not carry their foreflippers flexed over the
carapace, but instead held them laterally, edge on to water flow. During escape the
turtles dogpaddled. The powerstroke was used rarely (only when the turtles tried to
vigorously escape handling).
I have observed juvenile green turtles swimming along reefs in Hawaii and Florida,
and subadult loggerheads in Florida. All relied on the powerstroke for routine
swimming. No other flipper movement patterns were associated with locomotion in
these free-swimming animals. Captive C. mydas occasionally attempt to swim back
wards to escape a barrier. The mechanism is the same as described above. Captive
Chelonia mydas and Caretta caretta juveniles have been observed to push off the
bottom or move along the bottom using a modified dogpaddle stroke (diagonal limbs
moved together to push along the substratum).
Sea Turtle Locomotion: Mechanisms, Behavior, and Energetics 187
Data collected in Hawaii and Florida indicate that juvenile Chelonia mydas may
live relatively sedentary lives once they recruit to foraging and resting areas from
the open o c e a n . A d u l t green turtles from both Atlantic and Pacific populations
are known to migrate long distances. Migrations from feeding areas to nesting
beaches at French Frigate Shoals, part of the Hawaiian Islands, can exceed 100 km.
Balazs^^ estimates that these turtles cover 23 km/d. Mature green turtles marked at
Tortuguero, Costa Rica; Ascension Island, South Atlantic Ocean; and Southern
Yemen were subsequently recaptured after long-distance migrations. These mark-
and-recapture studies gave typical swimming speeds of 23.5 km/d, 21.6 km/d, and
22.4 km/d, respectively (summarized by Hirth).^"^ Prange^^ measured swimming
speeds averaging 1.4 to 3.6 km/h by adult green turtles (n = 10) as they swam away
from their nesting beach at Tortuguero, Costa Rica. Oliver^^ reported slightly higher
cruising speeds of 1.4 to 2.2 km/h by a captive adult C. mydas. Both Prange’s and
Oliver’s measurements of swimming speeds are considerably higher than those
calculated from mark-and-recapture data. In both cases they may have observed
sprinting rather than routine swimming. The highest published average speeds (53
to 65 km/d) are based upon mark-and-recapture of females after leaving the nesting
beach (Shultz,^^ summarized by Meylan).^^ However, since the routes taken by free-
swimming adults are not known (nor are the effects of cun^ents), the field estimates
may be too low or too high. Papi et al.^*^ tracked the post-nesting migration of an
adult green turtle by using satellite telemetry. Though the transmitter attached to the
shell increased the drag the turtle experienced, that increase is probably minimal
because the size of the turtle was quite large and the transmitter package was
relatively small and low in profile. The swimming speeds obtained (1.57 to 3.73
km/h = 37.7 to 89.5 km/d) were similar to those obtained from tag-return data, but
these estimates too do not account for displacements due to cuirents.
CarC and Parrish^'^ described swimming by captive adult loggerhead turtles.
Both indicated that the predominant method of thrust production was by the pow-
erstroke. Parish^^ noted that loggerheads typically completed 10 to 30 powerstrokes
per minute. Most published swimming speeds for this species are based upon
recaptures of marked turtles after a migration. These speeds ranged from 28.1 to
40.2 km/d.^°'^2 Tucker et al.^'^ obtained estimated swimming speeds from adult
female Caretta towing satellite transmitters. During their internesting movements
they averaged 0.45 km/h (range = 0.02 to 3.01 km/h). All free-swimming subadult
and adult Caretta (n = approximately 60) as well as juvenile and adult Chelonia (n
= approximately 84) that I observed swimming relied on the powerstroke. No
dogpaddling or rear flipper kicking has been observed in these sublittoral animals.
I observed that most swimming E. imbricata (juveniles and adults) in the waters
off South Florida used what appeared to be a typical cheloniid powerstroke. When
feeding, they occasionally crawl across the reef using diagonally opposite limbs. No
kinematic studies of hawksbill turtle swimming have been done and no quantitative
measurements exist of the thrust they generate from pushing against the water, the
hard bottom, or both.
Mark-and-recapture studies of E. imbricata suggest that juveniles are relatively
sedentary, though some long-distance migrations have been documented from
188 The Biology of Sea Turtles
The leatherback turtle is known for the longest migrations (both horizontally and
vertically). Mark-and-recapture studies of the adult turtles show that this species
regularly undergoes long-distance migrations (the maximum recorded migration
distance approaches 6000 km).'^^’^^ Reports in the range of 500- to 1000-km migra
tions are not uncommon. An estimated travel speed of 40 km/d was logged for a
turtle carrying a satellite transmitter after nesting at French Guiana.^^ A female
leatherback towing a satellite transmitter was tracked during its intemesting move
ments.^^ It traveled a minimum distance of 515 km, with a mean speed of 1.2 km/h
(= 28.8 km/d). As with other estimated speeds, no account of the effects of cuirent
was possible. Leatherback turtles are also noted for spectacular diving capabilities
which necessarily involve active swimming. Eckert et al.^"^ documented a maximum
dive to 1300 m by one mature female, and many other dives to less great depths
(around 200 m). Limpus^^ reported that a leatherback took an octopus bait in 50 m
of water.
Sea Turtle Locomotion: Mechanisms, Behavior, and Energetics 189
7.6 E N E R G E T IC A D A P T A T IO N S F O R M IG R A T O R Y
SW IM M IN G
Reptiles, generally, have low stamina and typically are capable of only short bursts
of high activityMarine turtles are obvious exceptions, as most swim for long
distances^^ and, in the case of hatchlings, for long durations without stopping."^"'
Aerobic Scope
c 0.028
1# Chelonia m ydas
w 0.024
1
■O
4> 0.020
E
3(ft
c
o
0-016 D erm ochelys
Q coriacea
C
0U)) 0.012
>
X
«
o 0.008
S 0.004
>O 0.000
FIGURE 7.13 Aerobic scope of three species of sea turtles. VO2 was measured for a 1.5-
h period (after a 30-min acclimation period) while the animals (i) swam in an open flow
respirometer or (ii) were rested quietly in a dark container. In all species maximum VQ was
measured during the swimming frenzy while hatchlings were powerstroking. Minimum VQ
was from resting measurements.
FIGURE 7.14 VO2 during frenzy swimming, postfrenzy swimming, and while resting. Each
reading is the mean ± SE for 10 animals. Mass specific O2 consumption was greatest during
frenzy swimming in Chelonia and Dermochelys, but not in Caretta. Swimming was aerobi
cally more expensive than resting, showing that aerobic metabolism is an important pathway
during hatchling migratory swimming.
192 The Biology of Sea Turtles
locate deep water and favorable currents). Once hatchlings distance themselves from
land they become less active swimmers. Their small size and relatively low thrust
prevent them from countering the effects of major currents. Once offshore they might
be viewed as swimming drift bottles, largely at the mercy of currents. In contrast,
adults have well-documented long-distance migrations between specific sites (e.g.,
feeding and breeding grounds). Their migrations sometimes involve swimming
against currents, clearly an energetically more expensive proposition. One might
cautiously suggest that adults are free to accommodate a much greater increase in
activity with less cost.
Jackson and Prange^^ noted that the breathing pattern of turtles on land (rest
with ventilation punctuating bouts of exercise) was different from that of swimming
turtles (single breaths). Hence, VO2 levels from nesting turtles may not be good
approximations of VO2 for swimming animals.
No data are available describing oxygen consumption by swimming Eretmo-
chelys, Lepidochelys, or Natator.
Aerobic measurements from adult Dermochelys are limited to data from adult
females during nesting, during post-nesting crawling, or restrained turtles (captured
just after nesting)."^^'^^ There are no data available for swimming animals. If breathing
patterns differ substantially between swimming vs. nesting or crawling (as described
for Chelonia), then aerobic measurements from nesting females may not be good
predictors of VO2 for swimming leatherbacks.
metabolism supplies a significant part of the energy for the offshore swim of
hatchlings. Hence, anaerobic glycolysis is clearly important to hatchlings during
most early phases of activity, both during digging to the surface of the nest and
during (at least) the early part of the swimming frenzy.
7.7 C O N C L U S IO N S
Sea turtles are the migratory specialists among the testudines. They possess a suite
of morphological, behavioral, and physiological characteristics that are adaptations
for sustained swimming. Unlike virtually all other turtle species, marine turtles
emphasize the mechanically efficient powerstroke as their primary swimming gait.
However, these migratory giants are forever tied to land for reproduction. Hence,
their morphological and mechanical traits must always reflect a compromise between
efficiency for the aquatic environment and effective for movement on land. Though
they spend a fraction of their lives on land, that portion of time is critical to
perpetuation of their genes, and hence is a strong selective pressure. Marine turtle
adaptations to the ocean environment are also constrained in another way. All of
their structural adaptations are built from and superimposed upon, a basic turtle body
plan (the armored tank, or box). Thus, they are also constrained by their ancestry.
During ontogeny, the ecology of the different life history stages changes as does
expression or emphasis of their adaptations for a migratory and marine life. This is
particularly evident in the ontogenetic shifts in locomotor behavior and mechanisms.
For example, the powerstroke is initially the primary gait of cheloniid hatchlings as
they distance themselves from land. It is largely abandoned in the pelagic (by some
species) where locomotion serves more for maneuvering during food gathering;
crypsis and/or carapace morphology provide protection. In the pelagic, dogpaddling
or rear flipper kicking predominate. The powerstroke returns as the primary gait
used by sublittoral (juvenile or subadult) life stages and adults. Activity patterns
(frenzy vs. routine swimming) change during ontogeny and correspond with eco
logical shifts and the associated migrations. The energetic costs of migratory swim
ming (and perhaps the energy pathways utilized: anaerobic and aerobic) not only
change with ontogenetic shifts in swimming behavior, but also may scale with body
size.
The cheloniid species are most similar in morphological, kinematic, and behav
ioral features. However, energetic adaptations may be more closely allied with the
ontogeny and ecology of a species than it is to phylogeny. Typically, cheloniids
switch swimming behavior and habitat several times in their life. For example,
Caretta caretta and L. kempp^ migrate away from predator-rich nearshore waters to
offshore nursery areas. Initially, they rely on a mechanically efficient (but for the
tiny hatchlings, energetically expensive) powerstroke and high levels of activity,
then, once in the pelagic environment their activity drops and their predominant
mode of propulsion changes. They remain pelagic animals for just a portion of their
lives, during which they grow considerably. They return to coastal waters as juveniles
and subadults. Their migrations along the coastlines are again executed using the
efficient powerstroke.
194 The Biology of Sea Turtles
Dermochelys hatchlings are pelagic migrants and remain so throughout life. This
turtle swims relatively slowly using the mechanically efficient powerstroke for very
long periods of time during routine swimming,and has low aerobic costs. Covering
long distances efficiently is critical. Its powerstroke, while different in detail from
that of cheloniids, is still a mechanically efficient gait. This gait is not abandoned
during ontogeny and persists largely unchanged.
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g Foraging Ecology and
Nutrition of Sea Turtles
Karen A. Bjornclal
CO NTENTS
8.1. IN T R O D U C T IO N
The closest interaction of an organism with its environment is the ingestion of a
subset of that environment and the subsequent alteration and absorption of that subset
as it passes through the digestive tract of the organism. The absorbed nutrients fuel
the productivity — both growth and reproduction — of the organism. The pivotal
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© 1997 by CRC Press, Inc 199
200 The Biology of Sea Turtles
role that nutrition plays in the productivity of individuals and populations — and
thus to the conservation of species — has often been overlooked.
Differences in diet, either in quality or quantity, are believed to cause the great
differences in mean growth rates of green turtles from different foraging areas in
the Hawaiian Archipelago.^ An herbivorous diet has important consequences for the
life history parameters and survival outlook of green turtles,and green turtles have
significant effects on the nutrient cycling and community structure of their seagrass
foraging habitats.A significant correlation between indices of the El Niño Southern
Oscillation and the numbers of green turtles nesting approximately two years later
at Heron Island and Raine Island is believed to have a nutritional basis, but the
mechanism is not known.^'-^ No major changes in seagrass abundance in the foraging
areas of these green turtles have been reported, so that if the cause is nutritional,
variations in quality rather than quantity of seagrasses may be responsible.^
Only by understanding the quantitative aspects of diet selection, digestive pro
cessing, and nutrition can we hope to understand the role of sea turtles in marine
ecosystems and to elucidate how nutrition acts as regulating mechanisms in the
productivity of sea turtles. As we make progress in descriptions of population
structure and in development of population models, an understanding of how nutri
tion regulates productivity will allow us to move to the next level and to address
how we can enhance or modify productivity to improve the survival outlook of these
endangered species.
Young green turtles are believed to occupy open ocean pelagic habitats, perhaps in
association with sargassum rafts in some areas, after leaving the nesting beach.
It is assumed that they are omnivorous with a strong tendency to carnivory during
this life stage.^ Records of diet from small pelagic green turtles are very few. One
hatchling, swimming away from a beach in Bermuda, dived down 1 m and ate a
Foraging Ecology and Nutrition of Sea Turtles 201
small ctenophored"^ The stomach of a green turtle (25.7 cm straight carapace length)
removed from the stomach of a shark caught 107 km off Durban, South Africa was
filled with the pelagic snail Janthina janthina}^ Plasma color provides additional
evidence of a carnivorous diet in pelagic-stage green turtles. When green turtles first
arrive on benthic foraging areas in the Bahamas, their plasma is unpigmented; after
they have begun feeding on seagrasses, their plasma has yellow pigmentation,^^ a
result of plant pigments.
Green turtles leave pelagic habitats and enter benthic foraging areas at a size of
20 to 25 cm carapace length in the western Atlanticand at 35 cm in Hawaii and
Australia. At that time they shift to an herbivorous diet, and, as herbivores, occupy
a feeding niche unique among sea turtles. Green turtles feed primarily on seagrasses
and algae, although they also consume animal matter, particularly jellyfish, salps,
and sponges. The early literature on feeding habits was reviewed by Mortimer.^^’^^
Few reports are available on the diet of green turtles in the Indian Ocean and
the Arabian Sea. Green turtles in the Gulf of Mannar and Palk Bay, India feed
primarily on the seagrasses Halophila ovalis, Thalassia sp., and the alga Gelidiella
acerosa; other seagrasses and algae were taken in smaller amounts as were squid
eggs and sponges.In South Yemen, the seagrasses Posidonia oceánica, Halodule
uninervis, Syringodium isoetifolium, and Cymodocea serrulata are the main diet
species for green t u r t l e s . G r e e n turtles in Oman feed on the seagrasses Halodule
uninervis, Halophila ovalis, Halophila ovata, and the algae Chaetomorpha aerea
and Sargassum illicifolium?^ Green turtles in the Seychelles Islands and in the
Comoro Archipelago feed primarily on Thalassodendron ciliatum and ingest smaller
amounts of other seagrasses and algae.
More data are available for green turtles on the Australian shelf. For green turtles
from Moreton Bay, Queensland, Australia, the mouth contents, in order of frequency
of occurrence, were the seagrasses Halophila ovalis, Halodule uninervis, Zostera
capricorni, Halophila spinulosa, and the alga Hypnea cervicornis; green turtles in
this area also feed on the jellyfish Catostylus mosaicus?^ In 44 green turtle stomachs
from Torres Strait, red algae were dominant (42 stomachs with >5% dry mass),
brown and green algae were equivalent (14 and 13 stomachs with >5% dry mass,
respectively), and seagrasses were present in 8 stomachs at >5% dry mass.^^ Based
on stomach contents from five green turtles feeding near Sir Edward Pellew Island,
Australia, the major diet species is the seagrass Halodule pinifolia with lesser
amounts of H. uninervis and Halophila spinulosa?^
Unfortunately, only preliminary results are available from the most quantitative
study to date on feeding habits and diet selection in green turtles.^® Based on stomach
lavage of 518 green turtles feeding on the reefs surrounding Heron Island, Australia,
the diet was composed of 38 species of red algae, 21 species of green algae, and 10
species of brown algae. Gelatinous animal tissue — Physalia and mollusc egg cases
— were also consumed.
Records of the diet of green turtles are available from a number of locations in
the rest of the Pacific. Algae comprise the diet of green turtles in the Ogasawara
Islands, J a p a n ; i n Tokelau, South Central Pacific;^^ and at Johnston Atoll.^^ The
diet of green turtles in the Hawaiian Archipelago includes 56 species of algae (of
202 The Biology of Sea Turtles
which 9 species are the major diet components), 1 species of seagrass, and 9 types
of i n v e r t e b r a t e s F i v e species of algae intentionally introduced into Hawaii for
aquaculture projects are consumed by green turtles.
Green turtles along the East Pacific coast may have more carnivorous diets than
those in other regions. Algae were found in all stomachs from 20 green turtles
captured in Peru, jellyfish in 12 stomachs, fish in 12 stomachs, and molluscs in 10
stomachs.Analysis of stomach contents from 39 green turtles (carapace lengths
52 to 89 cm) captured near Pisco, Peru, revealed the following percent occurrences:
molluscs (primarily Mytilus, Nassarius, and Semele), 64%; algae (mainly Gigartina
and Rhodymenia), 51%; polychaetes, 49%; jellyfish and commensal amphipods
{Hyperia medusarum), 31%; fish and fish eggs, 23%; salt grass {Distichlis), 18%;
and crustaceans, 13%.^^ The stomach of a large (71-cm carapace length) green turtle
captured in a pelagic habitat 40 nautical miles off the coast of Manta, Ecuador
contained fish eggs along with a small amount of sargassum to which the eggs had
been attached."^® The digestive tracts of 7 green turtles captured off the coasts of
Colima and Jalisco, Mexico contained green and red algae (4 turtles) and a great
diversity of invertebrates and chordates (all turtles)."^‘
In the Caribbean, the seagrass Thalassia testudinum is the primary diet species
for the green turtle.^ '^^ Thalassia comprised 87% of the dry mass of contents from
202 stomachs from green turtles captured on foraging grounds off the Caribbean
coast of Nicaragua; other seagrasses (Syringodium filiforme, Halodule wrightii)
made up an additional 5%."^^ On the southwestern Cuban Shelf, 14 green turtles had
fed primarily on the seagrass T. testudinum and the sponge Chondrilla nuculad"^
Along the coast of Brazil, algae are the primary diet.'^^ '^^ Green turtles in a lagoon
in Florida, fed primarily on the seagrasses S, filiforme and H. wrightii with lesser
amounts of the seagrass Halophila engelmanni and red and green algae.Small
green turtles in the waters of Long Island, New York fed on the seagrass Z. marina
and on 5 species of algae — 3 green and 2 brown.
For the first few years of life, loggerheads forage in open ocean pelagic habitats,
often — at least in the Atlantic — in association with sargassum. ^‘ Loggerheads
begin to move into shallower waters where they forage over a variety of benthic
hard- and soft-bottom habitats at a size of about 40 to 50 cm carapace length in the
Atlantic^® and at 70 to 80 cm on the Australian continental shelf and southwestern
Pacific basin.'’^ One or more large loggerheads were found associated with two
submarine geothermal springs on the West Florida Shelf, Gulf of Mexico.^^ Benthic
epifauna and nekton were considerably enriched in the area around the springs.
Diet items from post-hatchling loggerheads captured near the nesting beach have
been recorded off the coasts of South Africa and the southeastern U.S. Stomach
contents from 37 loggerhead hatchlings stranded on Cape Agulhas, South Africa,
yielded Physalia physalis, fragments of algae, bark, feathers, and fine grit that may
have been pieces of Janthina shells.Stomachs of five loggerhead hatchlings washed
ashore in Florida, in masses of sargassum during a hurricane contained sargassum
Foraging Ecology and Nutrition of Sea Turtles 203
floats and leaf parts, two snails (Litiopa melanostoma) that associate with sargassum,
the pelagic snail Diacria trispinosa, and pieces of crustacean appendages.^^ Two
dead loggerhead hatchlings, both with small yolk sacs, were found at the west wall
of the Gulf Stream 93 km east of Florida, and their guts contained three categories
of food: terrestrial insects, marine animals, and marine plants.The insects included
ants (winged, sexual forms), a fly, an aphid, leafhoppers, planthoppers, beetles, and
unidentified thorax fragments. The authors suggested that aerial insect plankton
settled on the ocean surface and were accumulated in convergence zones. Marine
animals that had been ingested were hydrozoan colonies, goose and acorn barnacles,
amphipods, crab zoea, shrimp eye capsules, planktonic fish eggs, and unidentified
muscle and chitinous fragments. Marine plants ingested were sargassum leaf and
bladder fragments and unidentified algal fragments. Major diet items flushed from
the stomachs of 42 post-hatchlings off the coast of Florida included gelatinous
animals (predominately medusae and ctenophores), crustaceans (primarily larval
shrimp and crabs), hydrozoans, insects, gastropods, sargassum, and an actinid anem-
one.^^
The diet of pelagic juvenile loggerheads has been recorded in several studies.
Brongersma^^' reviewed the early literature on feeding habits of pelagic loggerheads
in the Atlantic. The stomachs from loggerheads taken near the Azores and Madeira
contained salps, jellyfish, amphipods Hyperia medusarum that associate with medu
sae, pteropods Hyalaea tridentata, the crab Nautilograpsus {= Planes) minutus,
bunches of the barnacle Lepas anatifera, many sygnathid fish Entelurus aequoreus,
squid, and the pelagic snail Janthina. Brongersma^^ expressed doubt that loggerheads
could catch fish, but suggested that the slow speed of the sygnathid fish and the
mass mortalities of these fish that have been observed in the area could account for
the presence of these fish in the loggerheads. A careful analysis of the gut contents
of five juvenile loggerheads from the waters around Madeira, Selvagens Islands, and
the Azores revealed that the major diet components were pelagic coelenterates
(primarily siphonophores and to a lesser extent scyphomedusae and hydromedusae),
salps {Pyrosoma atlanticum), gastropods {Janthina spp. and Pterotrachea spp.),
barnacles {Lepas spp.), and the isopod Idotea metallicaJ'^ The jellyfish Pelagia
noctiluca is an important prey species for North Atlantic loggerheads."^^ Food items
from six pelagic-stage loggerheads (5.2 to 30.0 cm straight carapace length) that
stranded dead along the south Texas coast included sargassum, jellyfish, Janthina,
Litiopa melanostoma, and decapod and stomatopod larvae.^^ Pelagic loggerheads in
the North Pacific feed primarily on Velella velella and Janthina sp.^'^
Dodd^'^ presented a thorough species list of diet items found in the digestive
tracts of all life stages of loggerheads. Loggerheads feeding in benthic environments
ingest a wide range of invertebrates. Some of these prey items would seem to yield
relatively low nutrient gain, such as horseshoe crabs, Limulus polyphemus, which
is the most common prey item of loggerheads in Virginia.^^ Post-pelagic-stage
loggerheads feed throughout the water column, capturing jellyfish and salps at the
surface and middle depths, but they apparently concentrate their foraging efforts on
the bottom. Loggerheads in Moreton Bay, Australia create broad depressions in the
substrate with sweeping movements of the front flippers, thus exposing burrowing
204 The Biology of Sea Turtles
bivalves, such as Anadara trapezia, Pinna bicolor, and Solen grandis, which the
loggerheads then ingest.^* Loggerheads in this area also feed on the anemone Sti-
chodactyla haddoni and the crab Portuniis pelagicus.
Since the review by Dodd,‘’^ two quantitative analyses of loggerhead feeding
habits have been published. Feces were collected from 25 loggerheads captured in
the waters around Long Island, New York.^^ Crabs (Libinia emarginata. Cancer
irroratus, Pagurus pollicaris, and Ovalipes ocellatus) were present in the feces from
90% of the turtles, molluscs {Mytilus edulis and Busycon spp.) in 40% of the turtles,
and algae {Sargassum natans, Ulva sp., and Fucus spp.) in 20% of the turtles. As
noted by the authors, prey that undergo extensive digestion, such as jellyfish, will
be missed in a study based on feces. The stomach contents of another loggerhead
from this region yielded 18 seahorses {Hippocampus erectus), invertebrates, and
marine plants.
Digestive tract contents were analyzed from 82 loggerheads that stranded dead
in south Texas over a three-year period.^"^ The sea pen (Virgularia presbytes) was
the most common prey with a frequency of occurrence of 56% and comprising 59%
of the total prey dry weight. Crabs were the second most important prey item with
a frequency of occurrence of 88% and making up 29% of the total prey dry weight.
Nine species of crabs were identified; walking, or benthic, crabs were most impor
tant, while swimming (portunid) crabs were less commonly consumed. Molluscs,
tube worms, barnacles, fish, vegetation, sea pansies, whip corals, sea anemones,
mantis shrimp, penaeid shrimp, and Physalia physalis were also identified in the
gut contents. Significant dietary shifts occurred among seasons. Loggerheads fed
mainly on sea pens in the spring, and crabs were the major diet component in summer
and fall when crabs increase in abundance in the region.
Sponges comprised 95.3% of the dry mass of total contents from the digestive tracts
of 61 hawksbills from throughout the Caribbean.^^ Hawksbills feed very selectively:
98.9% of the dry mass of all identified sponges from hawksbills were from 3 of the
13 orders of demosponges (Astrophorida, Hadromerida and Spirophorida). Hawks
bills avoid sponges with spongin, but silica spicules apparently are not a feeding
deterrent. The 10 highest-ranked prey sponges (in descending order) were Chondrilla
nucula, Ancorina sp., Geodia sp., Placospongia sp., Suberites sp., Myriastra sp.,
Ecionemia sp., Chondrosia sp., Aaptos sp., and Tethya cf. actinia.
Studies conducted in Cuban waters report similar results. In 8 mature hawksbills
taken during the breeding season in the Gulf of Batabano, all had sponges in their
stomachs (all contained Chondrilla nucula, one had consumed T. diploderma, and
two had unidentified sponge species)."^"^ In a larger study of 73 hawksbills, sponges
comprised over 90% of the diet of all hawksbills between 50 and 80 cm.^^ Nine
sponge species were identified; the 5 most common were C. nucula, Chondrosia
collectrix, G. gibberosa, Erylus ministrongylus, and T. aurantia.
At Mona Island, Puerto Rico, demosponges have been found to comprise essen
tially the entire diet of subadult hawksbills.^^ Another study reported the gut contents
of six hawksbills from Puerto Rico: one adult had 95% of the sponge Chondrilla
niicula, one adult had 90% of the sponge G. neptuni, one adult had ingested only
the sea cucumber Holothuria cubana, the stomachs of two juveniles contained only
the sponge C. nucula, and one juvenile had fed on four species of demosponges.^^
The stomachs from an additional eleven hawksbills from Puerto Rico were full of
demosponges, with C. nucida the most common species.^"^ Other accounts of con
sumption of sponges by hawksbills in the Atlantic system are reviewed by Mey-
lan.66-67
Invertebrates other than sponges have been reported as major diet components
in the Atlantic, and marine plants have been noted in gut contents. Other than
sponges, tunicates were the major diet component of 20 hawksbills captured off
Tortuguero, Costa Rica.^'^ The Costa Rican hawksbills had also ingested bryozoans,
coelenterates, molluscs, and marine plants. The sea m tm ont Anemonia sulcata made
up the bulk of the gut contents of a hawksbill from Selvagem Pequeña (north of the
Canary Islands), which also included other coelenterates, sponges, oceanic squid,
gastropods, a sea urchin, a spider crab {Inachus sp.), and algae.^^ Several marine
plants were recorded in the digestive tracts of Caribbean hawksbills
Early diet records for hawksbills in the Pacific and Indian oceans suggested a
more omnivorous diet; large quantities of vegetation such as Sargassum, Rhizophora,
Cymodocea, and unidentified algae were reported from stomach c o n t e n t s . T w o
hawksbills from the Philippines had seagrass, sponges, and algae (Eucheuma and
Codium) in their stomachs.^^ WitzelP® summarizes other accounts of hawksbills
feeding on a wide range of diet items in the Pacific.
More recent studies, however, report that sponges form the preponderance of
the diet in the Pacific and Indian oceans. Hawksbills feed on sponges and soft corals
in the Seychelles,and sponges were dominant in the digestive tract from 35
hawksbills from Cosmoledo, Seychelles (J. Mortimer, personal communication in
Meylan).^*" An immature hawksbill from Mohéli, Comores had consumed four types
of demosponges.^^ The stomachs of two subadult hawksbills from Masirah Island,
206 The Biology of Sea Turtles
Oman contained only sponges.A mature hawksbill from Oahu, Hawaii had at least
three species of sponge in its digestive tract.^^ Sponges were the major food in two
hawksbill stomachs from South Africa and one from Pacific Panama.^'^ All reports
from the Pacific and Indian oceans are based on only a few turtles (other than the
personal communication of J. Mortimer); larger studies are needed to establish the
diet of hawksbills in the Pacific.
Hawksbills may undergo a period of omnivorous feeding in benthic habitats
before they adopt the specialized spongivory of larger juveniles and adults. Data
from three studies support this theory. First, two of the smallest individuals among
38 hawksbills studied by Meylan,^^ had significant quantities of nonsponge material
in their digestive tracts: a 23-cm hawksbill and a 26-cm hawksbill had consumed
invertebrates other than sponges, fish, and substrate (58 and 22% by dry weight,
respectively) in addition to sponges (42 and 78%, respectively). Three other hawks
bills in this size class had ingested 95 to 100% sponges. Second, feces collected
from a 33-cm hawksbill captured in the Miskito Cays, Nicaragua contained the red
alga Coelothrix irregularis (70% of the feces volume), tubes from two species of
polychaetes, sponge spicules, a hydroid, and pieces of a snail shell and a pelecypod
shell.Third, in Cuba, stomach contents were analyzed from 73 hawksbills ranging
in size from 30 to 90 cm carapace length.^^ Although sponges represented over 90%
of the diet for individuals larger than 50 cm, the stomachs from the two smallest
individuals had significant quantities of other foods. The red alga Gracilaria sp.
composed 84% (by weight) of the stomach contents from a 30-cm individual, and
the stomach from a 40-cm hawksbill contained 33% of an unidentified ascidean. In
both turtles, the rest of the stomach contents were sponges. Although the data in
support of a transitional benthic diet are limited, such a diet would support the turtles
as it adapts to the challenges — both mechanical (sharp silica spicules) and chemical
(toxic compounds) — of feeding on a sponge diet.
Purposeful ingestion of the coralline substrate^^ and substantial quantities of the
calcareous algae Halimeda incrassata^^ by gravid female hawksbills has been noted.
The authors suggest gravid females may consume these items as a source of calcium
for eggshell production.
Kemp’s ridleys apparently forage in surface waters during an early pelagic stage
that lasts until the turtles reach approximately 20 cm carapace l e n g t h . 84
size they move into relatively shallow water (less than 50 m) benthic foraging areas
with unconsolidated substrates.They will continue to forage in these areas, some
times moving long distances between feeding grounds.
The only report of diet of small, pelagic-stage Kemp’s ridleys is from two
individuals of less than 10 cm carapace length that stranded dead on south Texas
beaches.These turtles had consumed Sargassum sp., pelagic molluscs {Recluzia
rollandiana, Cavolina longirostris, and Litiopa melanostoma) that are often associ
ated with sargassum, and crabs that could not be identified.
Post-pelagic Kemp’s ridleys feed primarily on crabs. Crabs comprised the major
ity of the diet in 101 Kemp’s ridleys that stranded dead on Texas beaches with a
Foraging Ecology and Nutrition of Sea Turtles 207
percent occurrence of 1171% and percent of total dry mass of digestive tract contents
of 93.6%.^^ Other components were molluscs (62.4% occurrence and 2.2% dry
mass), fish (25.7 and 0.4%), vegetation (61.4 and 0.3%), and shrimp (8.9 and 3.2%).
The turtles consumed large quantities of both portunid crabs (Arenaeus cribrarius,
Callinectes sapidus, C. similus, Portunus gibbessii, and Ovalipes floridanus) and
other crabs (Hepatus epheliticus, Libinia sp., and Persephona mediterranea). This
observation contradicts the earlier idea that Kemp’s ridleys specialized on portunid
crabs; the distribution of Kemp’s ridleys may relate to the distribution of all major
crab species consumed rather than to only the distribution of portunids.^^ The small
quantities of vegetation probably were ingested incidental to other items in post-
pelagic turtles, and the small amounts of fish and shrimp ingested were probably
the result of scavenging on dead bycatch from shrimping vessels or discarded bait.^^
The presence of Nassarius spp. — molluscs that scavenge on dead tissue — in 15%
of those turtles that had consumed shrimp and in 50% of those turtles that had
consumed fish support the latter conclusion.
Kemp’s ridleys in coastal waters of New York State also feed primarily on
c r a b s . S p i d e r crabs (L. emarginatus) were by far the most commonly consumed,
followed by rock crabs {Cancer irroratus) and lady crabs {O. ocellatus). Molluscs
(Argopectin irradians, Mytilus edulis, and N. trivitattus) were consumed in smaller
quantities; small amounts of vegetation {Fucus sp., S. natans, Ulva sp., and Z.
marina) also were ingested. Seahorses {Hippocampus erectus) — a fish that relies
on crypsis rather than flight to avoid predation — comprised a substantial volume
of the gut contents in two Kemp’s ridleys from Long Island, New York.^^ Marquez-
lists other accounts of the feeding habits of Kemp’s ridleys.
The habitat of juvenile olive ridleys is not known; few sightings of size classes
between hatchlings and adults have been recorded.Adult olive ridleys apparently
utilize a wide range of foraging habitats. They feed in deep water, as indicated by
capture in bottom trawls at depths of 80 to 110 m.^^ They are common in pelagic
h a b i t a t s , a n d they feed in relatively shallow benthic waters, sometimes near major
estuaries.^'^’^^
The largest study of the diet of olive ridleys was conducted on 139 mature turtles
(115 females and 24 males) that were captured off the Escobilla nesting beach in
Oaxaca, Mexico.^^ Salps (all in the genus Metcalfina, possibly the species M. hex
ágono) and fish composed the largest percentage of the total volume of stomach
contents (45 and 42%, respectively), but occurred in only 14 and 5% of the turtles,
respectively. The other constituents were molluscs (5% volume and 66% frequency
of occurrence), crustaceans (4 and 48%), algae (2 and 51%), bryozoans (0.2% and
13%), and fish eggs, sipunculids, and ascidians (combined 0.1 and 11%). Percent
volume and occurrence varied greatly among months and between sexes. Given the
large sample size, the high volume percentages for some of the constituents with
low percent occurrence (i.e., fish were found in only 5% of the turtles, but accounted
for 42% of total stomach content volume) indicates that many of the stomachs must
have contained only a small volume of contents. Because these turtles were in a
208 The Biology of Sea Turtles
reproductive season, their stomach contents may not reflect the diet of olive ridleys
on their foraging grounds.
There are other reports for olive ridleys from Mexico. Jellyflsh and crabs were
the major components of the stomach contents of 20 adult females captured off the
state of GueiTero, Mexico.^^ Five olive ridleys, presumably captured in Mexican
waters, contained only Pleuroncodes planipes,^^ The stomachs of six olive ridleys
captured off the coasts of Colima and Jalisco, Mexico contained primarily benthic
invertebrates: crabs, pelecypods, and gastropods."^^ One turtle had consumed a scy-
phozoan medusa {Pelagia sp.).
Fritts^^ reviewed the notes of J.R. Slevin (1905 to 1906); Slevin reported that
fish eggs filled the digestive tracts of two female olive ridleys captured near the
Galápagos Islands, and small crabs filled the stomachs and intestines of two male
olive ridleys captured south of Cocos Island. The stomachs of two olive ridleys
captured about 40 nautical miles off Ecuador contained clumps of medusae."^^ Crabs,
jellyfish, and tunicates have been recorded in the stomachs of olive ridleys from the
eastern Pacific (unpublished reports cited in Mortimer).^ A mature female of 61-cm
carapace length found in New Zealand waters had been feeding extensively on
pelagic tunicates Pyrosoma and Salpa.^"^
The diets of olive ridleys in the Indian and Atlantic oceans are poorly known.
Deraniyagala^^ reported algae as the main diet component in olive ridleys from Sri
Lanka, although young pearl oysters and the sea urchin Clypeaster humilis were
also ingested. Stomachs from adult olive ridleys (number not stated) captured in
India were full of algae.^'’ The digestive tract from a female with about 200 ovarian
eggs caught at a 20- to 24-m depth off Surinam contained two small catfish, ten
snail shells, three small crab carapaces, and about 2 1 of what was believed to be
partially digested jellyfish.^^^
8 .2 .6 Flatback, N a t a t o r depressus
The flatback may be the only species of sea turtle that does not have an early pelagic
stage as surmised from the presence of remains of post-hatchlings (11- to 21-cm
carapace length) at island feeding stations of the white-bellied sea eagle, Haliaeetus
leucogaster!^'^’^^ The early post-hatchling stage is apparently spent within tens or
hundreds of kilometers of their natal beaches over the Australian continental shelf
where they inhabit shallow, turbid, weakly flushed, coastal waters protected from
ocean s w e l l s . P r i m a r y feeding habitat of larger flatbacks appears to be turbid,
shallow inshore waters off northeastern Australia and in the Gulf of Carpentaria,
most commonly in areas 5 to 20 m deep.^^ '^'^
The diet of the flatback is poorly documented. Stomach contents from two small
stranded flatbacks (14- and 22-cm carapace lengths) contained both planktonic
organisms (snails Janthina, siphonophores Porpina) and benthic organisms (corals,
molluscs, bryozoans), all of which could have been consumed in inshore waters.
Larger subadults and adults feed on jellyfish and on soft-bodied benthic invertebrates,
such as sea pens and soft corals, in habitats with unconsolidated substrates.
Foraging Ecology and Nutrition of Sea Turtles 209
The leatherback is believed to be the most pelagic of all sea turtles, spending much
time in the open oceanHowever, leatherbacks will forage close to shore and over
continental s h e l v e s a n d have been reported feeding in water of less than 4-m
depthd^^"^ The distribution of foraging leatherbacks appears to be largely dependent
upon the distribution of the jellyfish, salps, and other gelatinous organisms upon
which they feed^^^'^®^ and which often accumulate near convergent zones or water-
mass boundariesd^^ Leatherbacks feed throughout the water column, from the
s u r f a c e t o great depths. A leatherback fed on octopus used for fish bait at a
depth of 50 m in western Australia.^The presence of a deep-water siphonophore
(Apolemia uvarid) in the stomach of a Mediterranean leatherback suggests leather
backs may feed at great depths; however, this siphonophore may be carried to the
surface by upwelling water.^^ Internesting leatherbacks off St. Croix may feed on
siphonophores, salps, and medusae within the deep scattering layer at n i g h t ; t h e
mean nocturnal dive depths for six leatherbacks ranged from 31.8 to 83.9 m.
Because leatherbacks apparently inhabit the pelagic zone throughout their lives
and because the ability to capture and consume the gelatinous prey species is not
size dependent, there would seem to be no reason for a diet shift between size
classes, as is apparently the case in other sea turtle species. A 15.6-cm leatherback
captured 200 to 250 nautical miles southwest of Acapulco, Mexico had jellyfish in
its digestive tract.
Bleakney^**^ and Brongersma*^^ established that the leatherback feeds primarily
on scyphomedusae, pelagic tunicates, and their commensals, parasites and prey. The
five leatherbacks examined by Bleakney*^^ had consumed the jellyfish Cyanea capil-
lata arctica and its amphipod commensal Hyperia medusarum. Diet records since
that time have all supported the theory that leatherbacks specialize on gelatinous
organisms. The stomach of a leatherback captured near Malta contained three species
of pelagic coelenterates: the siphonophore A. uvaria, an unidentified siphonophore,
and an unidentified scyphozoan.^^ The gut contents of six leatherbacks from southern
England and the North Sea contained almost entirely scyphozoan medusae of the
species C. capillata, C. lamarckii, Rhizostoma octopus, Aurelia aurita, Pelagia
noctiluca, and Chrysaora hysoscella; the leptomedusa Ae^w6>rea sp.; portions of the
hydroid Obelia dichotoma; many amphipods (scyphozoan commensals) H. galba;
and fragments of a fish, a crab, and algae. Leatherbacks off the coast of France
feed almost entirely on R. pulmo}'^'^ Libinia spinosa, a small crab that is a jellyfish
commensal, was reported from a leatherback captured off the coast of Uruguay.
There are a number of records of leatherbacks feeding on Stomolophus meleagris
off the Atlantic coast of the U.S. and in the Gulf of Mexico. A leatherback was
observed feeding on Aurelia sp. off the coast of Washington, U.S.^^^ Leatherbacks
in Australia are reported to feed on the jellyfish Catostylus mosaicus}^^ Davenport
and Balazs*^'^ compiled records of leatherbacks consuming pyrosomas — pelagic
colonies of tunicate zooids — from the Pacific and Mediterranean.
210 The Biology of Sea Turtles
8.3 D IE T S E L E C T IO N
The development and duration of diet preferences in sea turtles are not understood.
Studies of early diet preferences in loggerhead hatchlings revealed that hatchlings
do forni preferences based on early feeding experience, but that these preferences
are reversible.
The extent to which the diet of green turtles is detemined by selective feeding
or by the relative abundance of different diet species has been addressed in several
studies. Green turtles in the Caribbean feed selectively by recropping plots of
Thalassia testudinum that they have previously g r aze d. By grazing on the short
leaves in the grazed plots rather than feeding randomly on Thalassia blades, they
ingest a diet that is higher in nitrogen and lower in lignin, a plant constituent that
is indigestible and lowers the digestibility of other structural carbohydrates in the
plants.
Fen'eira'^'’ attributed the high frequency of red algae in green turtle stomachs to
the greater abundance of red algae in the feeding areas. Balazs^^ suggested that both
relative abundance and feeding selectivity determine the feeding habits of Hawaiian
green turtles. Three small green turtles caught off Necker Island, Hawaii, feeding
over dense stands of Asparagopsis sp., Caulerpa sp., Laurencia sp., Sargassum sp.,
and Turbinaria sp., had only Caulerpa in their stomachs,suggesting they were
selecting that alga. In a series of foraging areas in Hawaii, the relative abundance
of available algae was quite different from the relative abundance of algae in stom
achs of green turtles.^"^ Sazima and Sazima"^^ reported that green turtles visually scan
the potential foraging area while slowly moving among algal patches. They suggest
that this scanning behavior and the differences between the algae available and the
algae consumed indicate that the turtles are feeding selectively. Ross^^ also attributed
the differential abundance of algal species in the habitat and in the diet of green
turtles in Oman to selective feeding. Garnett et al.^^ suggested that diet selection is
based on relative availability of diet species, although some selectivity for soft red
algae was detected in their study of stomach contents from 44 green turtles in Torres
Strait, Australia. In a preliminary report for a very thorough study of diet selection
in 518 green turtles that had ingested 69 species of algae near Heron Island, Australia,
Forbes^® concluded that green turtles did not ingest a diet based on relative abun
dances of the algae in the foraging habitat, but rather fed selectively.
BjomdaF '^^ hypothesized that microbial communities in the digestive tracts of
green turtles may affect diet selection, as suggested by three lines of evidence. First,
as reviewed by Mortimer,^ in many areas where both seagrasses and algae are present,
green turtles feed on either algae or on seagrasses, not on a mixture. Second, in
turtles (and dugongs) that feed primarily on seagrasses, algae appear undigested in
the feces in contrast to the very digested appearance of the seagrass (references in
Bjorndal)."^^ Conversely, in green turtles that feed primarily on algae, blades of
Thalassia in the posterior colon appear undigested, again in contrast to the very
digested appearance of algae surrounding them.^^^ Third, structural carbohydrates
in seagrasses are quite different from those in marine algae; structural carbohydrates
Foraging Ecology and Nutrition of Sea Turtles 211
also vary considerably among algae. The microbial communities in the digestive
tracts of green turtles feeding on seagrasses would differ from those in green turtles
feeding on algae. BjorndaP suggested that these differences in microbial populations
could affect diet selection because turtles with gut microbes adapted to algae would
digest seagrasses less efficiently, and vice versa. Although gut microbial communities
adapt to long-term diet shifts by varying the number and relative abundance of
microbial species/^^ turtles would digest food less efficiently if they made succes
sive, short-term diet shifts. There is a parallel situation in Orkney sheep that feed
on algae on North Ronaldsay Island and that are occasionally shifted to pastures of
angiosperms.^^"^'^^^
Specificity of the gut microbial communities may be one component in the
optimal foraging strategy of the green turtle, but it will not overwhelm all others.
The extent of the restriction on diet selection in green turtles that BjorndaP hypoth
esized has been overemphasized by researchers working with sea turtles (e.g., Gar
nett et al.).^^ Green turtles, like all organisms, select a diet that will yield the most
nutrition for the least investment in search and handling costs. When vast pastures
of seagrass or algae are available, the optimal forage for green turtles may be that
to which its gut microbes are adapted (either all seagrass or all algae). However,
where food is limited or where food types are more dispersed, the greater search
and handling costs of seeking either an all-algae or all-seagrass diet may be greater
than the energy gain from more efficient digestion. In this case, the turtle would
ingest a mixed diet.
Animals consistently ingesting a mixed diet would almost certainly develop a
microbial community capable of degrading the various complex carbohydrates. In
some areas of Australia, green turtles ingest both seagrasses and algae and, in the
feces, both components have the appearance of being equally digested.H ow ever,
the microbial populations in these turtles would have to adapt continually to any
changes in the proportions of seagrass to algae and in the proportions of the various
algae. Even if green turtles ingesting a mixed diet have lower digestive efficiencies,
their nutrient gain may well be maximized by the ability to ingest a greater quantity
of the mixed diet more rapidly.
In an assessment of selective feeding in Kemp’s ridleys,^^^ relative abundance
of four species of crabs (two swimming crabs [Ovalipes ocellatus and Callinectes
sapidus] and two walking crabs [Libinia emarginata and Cancer irroratus]) in the
foraging areas of Kemp’s ridleys in New York waters were compared with the
abundance of those crabs in the digestive tracts of Kemp’s ridleys. The turtles
ingested more walking crabs and fewer swimming crabs than would be expected
from the abundance of the crabs in the environment, indicating that the small ridleys
in New York waters feed on slower-moving prey perhaps because the turtles have
just moved out of the pelagic habitat and are inexperienced foragers in benthic
habitats. By the time these turtles have moved south into Chesapeake Bay, Virginia
they are more adept predators and are better able to capture the swimming crab
Callinectes sapidus, which is their primary prey species in that region.
212 The Biology of Sea Turtles
diet overlap was reported for juvenile Kemp’s ridleys and loggerheads in the waters
around Long Island, New York,^^ whereas larger size classes of the two species were
reported to exhibit habitat and diet partitioning in Virginia w a te rs .In one of the
few studies of diet overlap between sea turtles and other species, Sazima and
Sazima"^^ report that large herbivorous fishes {Acanthurus, Kyphosus, Sparisoma)
select different algae species than do green turtles in the same area, thus avoiding
competition.
All sea turtles serve as nutrient exporters from their foraging habitats. Some
species export nutrients on a daily basis by moving out of foraging areas to resting
habitats and defecating there. All species export nutrients on an annual basis when
mature individuals leave foraging habitats to migrate to nesting beaches where they
deposit substantial nutrient packets — in the form of eggs — that have been produced
from nutrients gleaned and stored on the foraging grounds.
8.5.1 I ntake
Intake — or the rate of food ingestion — has been measured in free-ranging green
turtles feeding on the seagrass T. testudinum in the Greater Caribbean in two stud-
ies.42,141 different approaches were used to estimate intake: BjomdaL^ used total
daily feces production and percentages of an indigestible marker in feces and in
Thalassia, and Williams used estimates of mean dive time, mean number of bites
taken per dive, amount of leaf shoot removed per bite, and mean number of hours
spent feeding per day. The two techniques yielded similar values for intake (Table
8 . 1).
Most physiological rates in poikilotherms are affected by temperature, and both
intake and passage rate in herbivorous reptiles are positively related to temperature.
Intake of trout pellets in post-hatchling green turtles increased significantly between
18 and 33°C: Qjo 18 to 23°C = 9.64, Q^o 23 to 28°C = 2.82, and Qio 28 to 33°C =
1 .97.^43
Diet quality also has a substantial effect on intake. Green turtles of similar size
feeding on a high-quality pelleted diet^"^"^ had intakes three to four times greater than
those for a diet of Thalassia (Table 8.1). These differences can have significant
effects on nutrient gain. Combining these intake levels with the higher nitrogen
content of the pelleted diet and the greater digestibility of nitrogen (see Tables 8.2
and 8.3), green turtles on the pelleted diet consume at least 12 times more nitrogen
on a daily basis than do green turtles feeding on Thalassia.
214 The Biology of Sea Turtles
TABLE 8.1
Intakes (g dry mass day~* turtle *) of Green
Turtles Feeding on Thalassia testudinum
Turtle size mean
(range) (kg) Intake Ref.
8 (7-9) 24 42
30 (28-32) 82 42
48 (46-50) 177 42
66 (64-68) 218 42
26 (4-60) 127 141
In the western Atlantic, green turtles typically have two feeding bouts each day
— one in the morning and one in the afternoon.'*^’'^^’*^'^However, in an area of reduced
food availability, Williams*"** found that green turtles fed continually over seagrass
beds during the nine h of daylight. In Hawaii, green turtles often feed at night; the
tidal cycle is more important in determining foraging times than is the diurnal
cycle. *^’^"*
The low nutritive value of jellyfish, as it pertains to the nutrition of leatherbacks,
has been repeatedly pointed oiit.**^ **^ The nutritive value of pyrosomas (gelatinous
tunicates) is apparently similar to that of jellyfish.**^ Large numbers of these gelat
inous organisms would have to be consumed to meet nutritional needs of a 900-kg
organism that is capable of maintaining body temperatures well above ambient. **^^’*"*'^
Duron*"*^’ estimated that an adult leatherback would eat about 50 large R. pulmo each
day (equivalent to approximately 200 1). A leatherback was seen to ingest 50 to 80
Stomolophus meleagris during a feeding session of unstated length off Myrtle Beach,
South Carolina.***"* The commensals that are ingested with the gelatinous organisms
or prey contained in their stomachs may be an important source of nutrition.**^
Six olive ridley hatchlings fed ad libitum on clam (Meretrix casta) tissue had
daily mean intake of 16.4 mg (SD 1.6 mg) dry mass per gram live body mass.*"*^
This dry mass intake was equivalent to a daily intake of 74.5 calories and 8.9 mg
of protein per gram live body mass.
8.5.2 D igestion
The extent to which food is digested — or digestibility — has been measured for
a number of diet components in wild green turtles feeding on natural diets of T.
testudinum‘^'‘^^'^'^'^ and the sponge Chondrilla nucula^^^ and for artificial diets in
captivity*"*"*’*^**’*'** (Table 8.2). The values for digestibilities from the study by
Bjorndal"*^ in Table 8.2 represent means of values measured in 12 consecutive months
in the southern Bahamas. There was no seasonal effect on nutrient composition of
Thalassia (Table 8.3) or on digestibility of Thalassia)'^ Among the four size classes
of green turtles, body size had a significant effect on digestion of organic matter,
energy, hemicellulose, and nitrogen, but not on digestion of cellulose.
Foraging Ecology and Nutrition of Sea Turtles 215
TABLE 8.2
Digestibilities Measured in Green Turtles on Several Diets: Seagrass
(Thalassia testudinum), the Sponge (Chondnlla nucula), and Pelleted
Diets
Digestibility Turtle size No. of
Constituent Diet (%) (*<g) turtles Ref.
39 30 3 42
45 48 3 42
54 66 3 42
Sponge 52 8 3 149
55 48 3 149
53 66 3 149
Pellet A 82-88 4-6 15 144
86-89 22-25 13 144
Pellet B 84 0.5-0.7 2 150
Pellet C 82 0.6-0.9 4 150
Organic nitrogen Seagrass 44 50 1 4
25 82 1 4
Experimental turtles in BjorndaE^ were the same as those in Bjomdal;^'^^^ turtles in BjomdaT^^
were the same as those in Thayer et alE
TABLE 8.3
Composition of Diets for which Digestibilities Are Presented in Table 8.2
Diet OM Energy Cellulose HC Lignin Nitrogen Si Ref.
All constituents are expressed as percent of dry matter except energy is kJ/g dry matter. OM is organic
matter; HC is hemicellulose; Si is silica.
The low nitrogen digestibility values for green turtles feeding on Thalassia may
result from a shift of protein digestion from the small intestine to the large intestine,
where absorption of end products is much less efficient.^ The extent to which cell
contents (including protein) are accessible to digestive enzymes in the small intestine
— before cell walls have been degraded — is not clear. The low nitrogen
digestibilities may be responsible for the low growth rates of sea turtles feeding on
Thalassia?
Foraging Ecology and Nutrition of Sea Turtles 217
First, the pelleted diets have higher concentrations of very digestible nutrients (nitro
gen, soluble carbohydrates) and lower concentrations of less digestible constituents
(structural carbohydrates) than do seagrasses and algae. Second, because of these
shifts in nutrient concentrations, the pelleted diets are digested to a greater extent
by endogenous enzymes in the small intestine, whereas algae and seagrasses are
digested to a greater extent by microorganisms in the large intestine.^’^^^ Digestion
in the small intestine results in more efficient and complete nutrient absorption.
Third, particle size of the pelleted diets is very fine, compared with the large particle
size that is ingested by green turtles feeding on seagrasses and algae. Because turtles
cannot chew their food, particle size of ingested seagrass and algae are typically
several centimeters square. Particle size of digesta has a significant negative effect
on rates of fermentation and digestion in herbivorous reptiles and mammals.
Habitat degradation resulting from these practices decreases the quantity and quality
of foraging habitats for sea turtles.
A concern that has not yet been addressed is that of direct competition for food
between humans and sea turtles. Recent estimates^^^ indicate that commercial fish
eries in continental shelf systems have a high primary productivity requirement of
24 to 35%, which may not be sustainable and may threaten the biodiversity of these
systems. In addition, a higher primary productivity requirement would starve top
predators such as marine mammals and b i r d s . A s commercial fisheries become
increasingly efficient, and as more commercial species of fish and invertebrates
approach commercial — if not biological — extinction, sea turtles that feed on these
commercial species may suffer reduced feeding rates because of limited food
resources. Those sea turtle species that do not feed on commercial species may find
that availability of their dietary species have been negatively affected through
bycatch in commercial fisheries or as a result of the disruption in marine food webs
caused by the dramatic population/species shifts and habitat degradation resulting
from commercial fisheries. The low primary production requirement of ectothermic
sea turtles relative to endotherms may provide some protection from competition
with humans.
The sublethal effects of food limitation resulting from such competition will be
difficult to discern, but may reduce significantly the productivity of sea turtle pop
ulations by lowering growth rates, delaying onset of sexual maturity, and reducing
reproductive output. To monitor these sublethal effects, baseline data on rates of
growth and intakes on natural diets are needed for many populations of each sea
turtle species. These populations should represent a broad geographic range and all
habitat types within each species. Only with this information can the amount of food
resources necessary to sustain populations of sea turtles be determined.
If a population limited by food availability was released from that food limitation,
it has been suggested that compensatory gain may act to increase productivity and
hence population recovery.Compensatory gain is realized when an organism that
has had poor nutrition — often as a result of limited intake or poor quality food —
is provided better nutrition. For a period of time, the previously undernourished
individual will have a lower feed-to-gain ratio than an individual that has been
maintained on the better diet throughout its life. Compensatory gain is well known
in mammals, but has not been studied in reptiles.
Deposition of silt on coral reefs, rocky bottom habitats, and seagrass beds —
most commonly as a result of inappropriate land management practices — decreases
the amount of foraging habitat available to sea turtles. Oil spills are a constant threat
to sea turtle foraging habitats. The Kemp’s ridley is particularly vulnerable because
the two major feeding areas of adult ridleys are in areas of intense development for
offshore oil production in the Gulf of Mexico.
In a different twist, construction of offshore oil platforms, particularly in the
Gulf of Mexico, creates hard-bottom communities that are favored by foraging
loggerheads. These structures — which currently number approximately 3800 and
provide the equivalent of about 5000 km^ of hard bottom, increasing the amount of
that habitat in the Gulf of Mexico by 27% — may remain in place for decades and
acquire a complex community of invertebrates.W hen the oil platforms are
removed (currently at a rate of about 100 per year), as required by law, most are
removed with explosives that destroy the habitat and can kill turtles in the area.^^^’^^^
8.7 C O N C L U S IO N S
Much research is needed to elucidate the relationships between the foraging ecology
of sea turtles and their role in marine ecosystems and between the nutrition of sea
turtles and their productivity. Surprising gaps remain in our knowledge of feeding
habits. Some of these gaps can be generalized to ocean basins, species, and life
stages. There is a paucity of data for all species from the Indian Ocean, for the early
life stages of all species, and, among species, the diet of the flatback and the olive
ridley are the least known.
The study of nutrition in sea turtles is in its infancy. Studies are needed on all
aspects of intake, digestion, and passage of digesta and on quantitative nutritional
requirements. To the extent possible, these studies should involve natural diets and
turtles that have not been raised in captivity.
Although understanding diet selection is critical for assessing habitat quality —
an undertaking that is ever more critical as wildlife managers are expected to make
difficult decisions on which habitats to protect and which to abandon — such studies
are still rare. Quantitative studies are needed that address diet selectivity in sea turtles
by relating relative abundance of potential food items in the environment to the food
items that are ingested.
We have failed to evaluate adequately the role of sea turtles in their ecosystems.
Such studies are essential not only to improve our understanding of how marine
ecosystems function, but also to assess what is lost at the ecosystem level when sea
turtle populations become severely reduced or extinct.
Finally, the negative effects of human activities on the foraging habitats of sea
turtles and their diet quality and availability must be quantified and controlled. Such
quantification will be difficult because many of the negative effects are sublethal or
are indirect results of changes in the delicate balance of marine ecosystems, and
thus difficult to trace.
222 The Biology of Sea Turtles
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tissues of sea turtles exposed to the Ixtoc I oil spill,/. Wildl. Dis., 19, 106, 1983.
170. Hutchinson, J. and Simmonds, M., Escalation of threats to marine turtles. Oryx, 26,
95. 1992.
Foraging Ecology and Nutrition of Sea Turtles 231
171. Lutz, P. L., Studies on the ingestion of plastic and latex by sea turtles, in Free. 2nd
bit. Conf. Marine Debris, Shomura, R. S. and Godfrey, M. L., Eds., NOAA Tech.
Memo. NOAA-TM-NMFS-SWFSC-154, Honolulu, HI, 1990, 719.
172. Schulman, A. A. and Lutz, P., The effect of plastic ingestion on lipid metabolism in
the green sea turtle {Cheionia mydas), in Proc. 12th Anna. Workshop on Sea Turtle
Biology and Conservation, Richardson, J. I. and Richardson, T. H., Compilers, NOAA
Tech. Memo. NMFS-SEFSC-361, Miami, FL, 1995, 122.
173. Pauly, D. and Christensen, V., Primary production required to sustain global fisheries,
Nature, 374, 255, 1995.
174. Wetherall, J. A., Balazs, G. H., Tokunaga, R. A., and Yong, M. Y. Y, Bycatch of
marine turtles in North Pacific high-seas driftnet fisheries and impacts on the stocks,
North Pac. Comm. Bull., 53, 519, 1993.
175. U.S. Fish and Wildlife Service, and National Marine Fisheries Service, Recovery
plan for the Kemp’s ridley sea turtle {Lepidochelys kempii). National Marine Fisheries
SeiTice, St. Petersburg, FL, 1992.
176. National Research Council, An Assessment o f Techniques fo r Removing Offshore
Structures, National Academy Press, Washington, D.C., 1996.
177. Bjorndal, K. A., unpublished data.
178. Limpus, C., personal communication.
179. McCauley, S. J. and Bjorndal, K. A., unpublished data.
Q Age, Growth, and
Population Dynamics
M. Y. Chaloupka and John A. Musick
0-8493-8422-2/97/$0 00+$.50
© 1997 by CRC Press, Inc. 233
234 The Biology of Sea Turtles
9.1 INTRODUCTION
Many sea turtle stocks are in decline worldwide due to excessive harvesting and
destruction of nesting and foraging habitats.^ Yet population abundance for many
stocks can display stochastic fluctuations with no apparent long-term trend. How
are we to know then whether a stock is really at risk and what risk management
strategies might be appropriate for protection of the stock? These are challenging
questions that cannot be resolved without a thorough understanding of sea turtle
growth and population dynamics. Age and size are related components of somatic
growth, which itself is a major determinant of population dynamics and macroevo-
lutionary change.
Therefore, we review in broad terms a wide range of studies on age, somatic
growth, and the population dynamics of sea turtle stocks. Firstly, we present an
overview of some of the major methodological issues relevant to understanding sea
turtle growth and population modeling. This section is essential to appreciate the
methodological themes apparent in the remaining sections. Many of the methods
used need careful scrutiny of the underlying statistical properties and assumptions.
Therefore, the main emphasis in this review is on methodological issues.
Then we review critically a compilation of sea turtle growth studies arranged in
a classification table providing a framework for grouping similar approaches. Next
we discuss the complementary approaches of comparative life history analysis and
comparative demography as a precursor to population dynamics. In the last section
we review critically all known studies on sea turtle population dynamics, also
arranged in a classification table. We conclude with a brief overview of the review
focusing on recurrent methodological issues.
Age, Growth, and Population Dynamics 235
Ecological studies are based on many presuppositions including two key sampling
design issues — (1) the stock is distinguishable over time and space and (2) the
demographic processes of that stock are time invariant. The first design issue is
reasonably well understood, but the second issue of implicit time invariant sampling
is very poorly understood in most ecological research, let alone in sea turtle studies.
Estimates of demographic rates such as mortality, growth, and recruitment rates are
a key part of modeling population dynamics. However, demographic processes are
236 The Biology of Sea Turtles
Metal tagging of flippers is the most common approach used for mark-recapture
programs designed for long-tenn monitoring of individual t u r t l e s . O v e r time
a detailed mark-recapture profile is compiled for each turtle comprising morpho
metric measurements at first and subsequent recaptures, time of first capture, time-
at-large since first capture, and in some cases determination of sex, maturity, and
reproductive status derived from visual examination of gonads using laparoscopy.^^’^^
These profiles provide the basis for estimating demographic parameters needed
for population modeling such as mortality r a t e s , r e c r u i t m e n t ra te s ,in te r
habitat dispersal rates,^^ annual remigration rates,^'^ and sex-, size-, and age-specific
growth rates.^^’^^‘^ The collection of demographic information on individual turtles
requires a reliable means of capture and marking to enable recognition at recapture.
Clearly, the reliability of the marking methods is a concern for long-term mark-
recapture programs.
Age, Growth, and Population Dynamics 237
Most sea turtle monitoring programs use multiple tagging with subsequent replace
ment protocols, although a few have relied on single tagging such as for green turtles
nesting in Malaya and Saraw ak,green turtles nesting at Tromelin and Europa in
the Indian Ocean,^^ and for recaptured headstarted Kemp’s ridleys released in the
Gulf of Mexico.^^ While many long-term sea turtle monitoring programs have used
multiple tagging protocols, most of them have also used monel alloy tags, which
are known to corrode rapidly in seawater.'^^ '^'^ Clearly, studies that used single monel
tagging^^’^^’^'^’^^"^^ are most at risk, although Caillouet et al.^^ have also used single
inconel tags, and Limpus et al.,^’^ Limpus,^^ and Frazer et aU^^ have long since moved
to multiple titanium or inconel tagging. Single tagging, even if it is an inconel tag'^^
or a titanium tag,^® is not a prudent practice. Plastic tags are known to deteriorate
rapidly in seawater, probably resulting in low tag retention rates.
Both inconel and titanium tags have outstanding long-term durability in seawa
ter. 18,44 However, both monel and inconel are alloys containing nickel, which is a
known antifouling agent. Titanium, on the other hand, has no antifouling properties,
although it also has no known toxic properties. Limpus'^'^ has noted that titanium
tags on recaptured sea turtles in the Great Baixier Reef are often covered with
barnacles, which he attributes to potential tag loss. Wood and Wood^^ suggested an
association between titanium tags and fibropapillomas on recaptured headstarted
green turtles released from the Cayman Islands. Studies using both inconel and
titanium tags^’^’^®conducted in accordance with a properly designed experimental
238 The Biology of Sea Turtles
protocol could help to resolve these issues. Clearly, more research on tag design,
material technology, and tag retention rates is needed, but it might well be that
inconel and perhaps titanium are the preferred materials for long-term monitoring
of sea turtle stocks.
9.2.3 Skeletochronology
Periodic marks or growth checks on hard parts such as scales or bones have been
used to estimate vertebrate age for almost 100 years.^* The procedure assumes
amongst other things that (1) a constant proportional relationship exists between
hard part growth and somatic growth and (2) that discernible periodic growth rings
or annuli are deposited at regular constant time intervals. Zug et al.^^ reviewed the
history of the use or attempted use of bony rings to estimate age (skeletochronology)
in sea turtles. The future of skeletochronology for estimating sea turtle ages rests
with resolving three issues — (1) validation of universal annual deposition of growth
layers, (2) development of a robust method to correct age estimates for growth layer
loss, and (3) validation of constant proportional allometry between bone growth and
somatic growth to enable application of length-at-age back calculation methods.
Age, Growth, and Population Dynamics 239
Zug et al.^^ used the humeral bones to estimate age and growth for northwestern
Atlantic loggerheads. They assumed that growth zones apparent following histolog
ical processing of the bone were deposited on an annual basis, although others^^ had
mixed success previously in validating the annual nature of bony rings in sea turtles.
Klinger and Musick"^^ have validated the annual nature of the humeral growth
layers in loggerheads using OTC injection. Several turtles were subsequently
stranded dead or were recaptured live by fishermen after being at large for up to
four years. Humeri were removed from dead animals and processed according to
Zug et al.^^ The number of growth rings deposited beyond the fluorescent OTC
benchmark were then counted. Live recaptured turtles were anaesthetized and
humeral bone biopsy samples were taken and histologically processed as in whole
humeral sections.^"^ The results of this study showed that one growth layer was
deposited for each year a turtle was at large for this stock after OTC marking.
Annular growth zones in the humeri of a wide size range of western North
Atlantic loggerheads have now been validated. In addition, growth zones have been
validated in a small sample of Lepidochelys kempi?^^ It remains to be seen whether
such zones are formed on an annual basis over the entire size range of L. kempi, or
for small pelagic loggerheads.
Frazier^^ also used the OTC method to attempt to validate the nature of growth
zones in a captive group of green turtles, but had poor results. Zug et al.^^ suggested
that this was likely because the growth rates were accelerated in captivity and so
that the normal pattern of bone growth might have been disrupted. This is an
important point because supposedly aseasonal environments such as the tropics may
not induce strong seasonal pulses in growth commonly found for more warm tem
perate species such as loggerheads or Kemp’s ridley — the two species subject to
most aging studies. Annular layers have yet to be validated for sea turtle species
with a tropical distribution pattern such as greens and hawksbills.
Growth layer resorption in skeletal elements has been found to occur in post-pelagic
growth phases of loggerheads, Kemp’s ridleys, and leatherbacks.'^^’^^’^^ ^’^ Zug et al.^
have also shown that growth layer resoiption also occurs in pelagic loggerheads less
than 20 cm curved carapace length (ccl) or about 2 years old caught in the North
Pacific high seas driftnet fishery. Therefore, it seems likely that growth layer resorp
tion occurs in all postnatal growth phases of sea turtles.
Zug et al.^^ proposed originally a simple absolute growth rate averaging method
to compensate for growth layer loss when determining age estimates for post-pelagic
loggerheads. However, this method may overestimate the number of layers because
growth may be faster and growth layers may be wider in younger, fast-growing
turtles than in older, slower-growing turtles.O ther adjustment methods to correct
for growth layer loss have since been developed and are now more widely applied
— (1) correction factor p r o t o c o l , (2) regression growth protocol,^'^^ and (3)
240 The Biology of Sea Turtles
the ranking protocol.^ '’^-'^^ '’^ The correction factor and regression growth protocols
are based on several assumptions related to constant relative growth rates predicated
on a von Bertalanffy growth function and in some cases application of the Fraser-
Lee back calculation function The use of the Fraser-Lee BCF is also
discussed in the next section concerning length-at-age estimation. The Zug ranking
protocol'’'^is simpler and based on fewer assumptions. Nonetheless, all three methods
require further validation and development to warrant confidence in the age estimates
derived using these adjustment methods.
The standard Fraser-Lee back calculation method was used to derive loggerhead and
Kemp’s ridley length-at-age records.^^’"^^’^^’^^ The specific reason for back calculation
other than for growth layer loss adjustment was unclear, but is presumed to be for
increasing the sample size for fitting an age-size growth function. The sample could
also include age-length estimates for lengths not observed in the study. Length back
calculation derives multiple age-length measurements for each individual, depending
on the ontogenetic stage reached by the individual at time of biopsy or death. Back
calculation in theory, then, represents mixed longitudinal sampling, which is desir
able. However, it has been common practice to use a standard Fraser-Lee BCF
providing only a length estimate at the earliest discernible growth mark.^^’^^’^^’^^ This
practice yields only mixed cross-sectional data at the expense of discarding growth
information.
Zug et al.,^^ in an analysis of Kemp’s ridley growth, also used Fraser-Lee-derived
back calculated lengths for fitting the Fabens size-based analogue of the von Ber
talanffy growth function. The Fabens analogue is not appropriate for determining
an age-length function^® (see Section 9.2.4.3). Moreover, the parameter form of the
von Bertalanffy growth function fitted to all these age-length data sets is known to
have poor statistical p ro p e rtie s .T h e s e issues are discussed in more detail in
Section 9.2.4.
There appears to be much confusion in the sea turtle skeletochronological liter
ature regarding the correct approach for using proportional BCFs. The main problems
seem to be due to a misunderstanding of the following: (1) implicit proportionality
hypothesis, (2) correct form of the standard Fraser-Lee equation, (3) potential of the
general Fraser-Lee equation to derive multiple length-at-age measurements per indi
vidual, and (4) correct linear regression to use, depending on the particular BCF used.
Assuming a scale proportional hypothesis, a regression of the data set comprising
the humerus radial length (y variable) and straight carapace length (x variable)
recorded from each individual at capture should be applied.^^ Ricker^“^ argues that
the order of (y) and (x) is irrelevant so long as a symmetrical linear regression model
such as the reduced major axis model is used to derive the parameter estimates. If
the fitted functional relationship is linear, then it is possible using the regression
parameter estimates to derive back calculated length estimates for a specific indi
vidual at all previous ages indicated by the growth checks using an appropriate BCF,
such as the general Fraser-Lee equation.^^ This generates a set of length-at-age data
with variable number of length-age records per individual for fitting an age-size
Age, Growth, and Population Dynamics 241
growth function. There is, of course, no necessity to use a regression model in the
first place, since biologically determined values where available could just as validly
be used in any proportional Irrespective of method, current BCFs do not take
into account the time-dependent nature of the relationship between skeletal and
somatic growth rates. Future sea turtle aging models using skeletochronology and
back calculation will need to evaluate the assumptions of proportionality and time-
invariant growth rates.
9.2.4 G rowth
The main purpose of growth studies when age is known has been to fit a parametric
growth function useful for estimating important demographic processes such as
maturation rate. The von Bertalanffy growth function has been widely used in sea
turtle growth studies (see Section 9.3). The age-based form of this function has no
242 The Biology of Sea Turtles
inflexion representing a monotone declining growth rate as the turtle ages through
the postnatal development phase, irrespective of whether absolute or specific growth
is used. Interestingly, of all the studies on sea turtle growth purporting von Berta-
lanffy growth, only two studies^^’^^ showed a monotone declining size-specific
growth rate, and then only for part of the postnatal development phase.
The von Bertalanffy growth function has very poor statistical properties resulting
in misleading and biased growth parameter estimates.^’ The same problems apply
to the generalized von Bertalanffy or Chapman-Richards function^^ used in a known-
age growth study of West Atlantic immature green turtles (see Table 9.1). An even
more generalized model known as the Richards function has been shown to have
the worst statistical properties of any known function.T his function, also known
as the generalized logistic or Richards-Nelder function, has not yet been used in sea
turtle growth studies.
Ratkowsky^^ has shown that other common parametric age-based growth func
tions used in sea turtle growth studies such as the logistic^^ and Gompertz^'^ functions
have excellent statistical properties. The sex-specific Weibull functions used by
Chaloupka and Limpus^^ also have good statistical p ro p erties.O f course, this
applies so long as the function is fitted to data spanning the whole postnatal devel
opment phase.
Ratkowsky^^ has also shown that the statistical properties of many nonlinear
parametric growth functions can be improved significantly by using expected-value
parameters. This involves replacing some function parameters with known or fitted
response variable values that correspond with, say, the lowest and highest known
values of the explanatory variable. Say for instance that the response variable was
ccl with known age (years) as the explanatory variable, then initial estimates of
suitable expected-values can be read from a nonparametric regression smooth^'^ of
the bivariate data plot. The initial estimates would replace unknown parameters in
a reparameterized form of the function being used. Recall that this concept of using
biologically relevant values was also suggested for length-at-age back calculation
procedures (see Section 9.2.3.3).
Schnute and Fournier^® and Ratkowsky^* provide expected-value parameter
forms of the von Bertalanffy function. All reparameterizations of the von Bertalanffy
growth function have excellent statistical properties.^^ A point implicit in the
expected-value approach is that growth functions are only fit to the range of observed
data. Extrapolating any function beyond the empirical range is fraught with diffi
culties. This principle applies irrespective of the growth function used, but has not
been well adhered to in sea turtle growth studies (see Section 9.3).
^ Explicit mixed cross-sectional; ^ Implicit mixed longitudinal, but mixed cross-sectional due to insufficient recaptures; ^ Reanalyzed here; von Bertalanffy function fitted
to left-censored data; ^ Specific growth rate metric; ^ See also Limpus and Chaloupka.^^^
to
Ü1
246 The Biology of Sea Turtles
These analogues have been used for two main purposes — (1) to derive growth
parameter estimates from absolute or specific growth data sets provided from mark-
recapture studies and (2) to substitute these parameter estimates into an analogous
age-based growth function to generate a size-at-age function. There are two major
problems with both practices. Firstly, the poor statistical behavior of the von Ber-
talanffy function has also been shown for the Fabens a n a l o g u e , a n d using similar
arguments it follows that the logistic analogue^^ would have poor properties.
Francis^^ gives an expected-value reparameterization of the Fabens analogue while
Francis^'’ gives an analogue of Schnute’s general growth model,^^' which can also be
used in expected-value form. Bear in mind that these models provide growth-at-size
functions, not growth-at-age functions. Secondly, the widely accepted practice in
sea turtle studies of parameter substitution (see Section 9.3.2.3.3) is inappropriate
because size-based growth parameters are not equivalent to age-based growth param
eters — they are analogous, but not equivalent as clearly shown by Francis.^^ Put
simply, replacing the growth parameters in, say, the von Bertalanffy age-based
function with parameter estimates derived from either the Fabens^ ^ or Kaufman^^
size-based analogues is invalid. The same applies to use of all other analogues. The
only apparent way around this problem when age is unknown is to use numerical
integration to derived separate growth-at-size and growth-at-time-at-large functions.
This could also be done using a Francis analogue,^^ but, as discussed below, ana
logues cannot take into account the confounded time-dependent nature of growth
data unlike the statistical modeling and numerical integration approach of Chaloupka
and Limpus^^ and Limpus and Chaloupka.
Finally, a point relevant to statistical fitting of age- and size-based growth
functions concerns goodness of fit. It is common practice in sea turtle growth studies
using nonlinear regression to use R-square as a numerical goodness of fit measure.
This is a fallacious practice (Ratkowsky^'^ and references therein). Goodness of fit
for nonlinear regression is a matter of judgment based on residual variance, exam
ination of residuals, and assessment of underlying assumptions.
93 G R O W T H M O D E LS
A wide selection of sea turtle growth studies have been arranged in Table 9.1 into
one of twelve groups according to the following factors: (1) whether the main focus
was on age or size, (2) the implicit sampling design (cross sectional, longitudinal,
mixed longitudinal), and (3) the specific analytical method used. Table 9.1 includes
the citation, species, ontogenetic range, location, growth metric, and underlying
explicit or implicit growth behavior. We discuss the groups below, but first we raise
four major issues relevant to a critical review of all groups.
Firstly, sea turtle development undoubtedly comprises separate growth compart
ments representing distinct ontogenetic phases — Ricker^'“’ refers to ontogenetic
phases as growth stanzas. Just how many phases is unknown but at the very least
there are distinct prenatal and postnatal developmental phases. The number of phases
within the postnatal period will depend on the ontogenetic habit of each species.
For instance, the flatback {Natator depressus) has no epipelagic development phase,
but remains in a neritic habitat.^^ Notwithstanding individual growth variability.
Age, Growth, and Population Dynamics 247
distinct developmental phases are most likely to have different somatic growth
characteristics.^® However, it has been a very common practice in sea turtle growth
studies to fit a monophasic growth function to a limited ontogenetic range assuming
that the chosen parametric function reflected the entire (rarely observed) postnatal
development period. There is simply no substantive basis for such a practice. Most
of the growth studies shown in Table 9.1 that fitted growth functions have done this,
although some studies such as those of Bjorndal and Bolten^^ have long recognized
this as an inappropriate practice.
Secondly, another widespread practice (Table 9.1) has been to discard multiple
growth measurements per individual, thereby turning implicit mixed longitudinal
sampling into explicit mixed cross-sectional sampling (see Section 9.2.1.2; Table
9.1 footnotes; also Fitzhugh^^ for a discussion of data types). The usual approach
has been to use only data from first capture and last recapture and discard recapture
events (if any) in between. The same approach is prevalent in skeletochronological
work where growth marks between the earliest and the last are disregarded in growth
measurement for growth layer adjustment procedures. There is no apparent basis
for this practice other than perhaps a misunderstanding that repeated observations
on some individuals and not others in a data set is invalid. It is not, and in fact mixed
longitudinal sampling (sampling with partial replacement) has many desirable
properties — so why throw away useful growth data? Thirdly, a recurrent
although not widespread practice that is inappropriate in sea turtle growth studies
is the discarding of zero or negative growth measurements prior to analysis. We can
find no statistical basis for such a practice. Lastly, it must be borne in mind that
many studies shown in Table 1 were based on mark-recapture programs using
unreliable tag types and tagging protocols (see Sections 9.2.2.2 and 9.2.2.3 above).
The 18 studies classified as age-focused in Table 9.1 used either known-age turtles
or estimated age using skeletochronology (see Section 9.2.3). All three sampling
designs (cross-sectional, longitudinal, and mixed longitudinal) were used, reflecting
the diversity of study settings and research purposes. If skeletochronology is coupled
with back calculation (see Section 9.2.3.3), then it is mixed longitudinal sampling,
else it is cross-sectional sampling, which confounds age and cohort effects.
heads (see also Bowen et al.^) and suggests that the pelagic phase for some stocks
may well span 10 or more years.
Growth mark validation, growth layer loss correction, and length back calcula
tion are major problems for all skeletochronological studies, and growth findings
need to be considered cautiously (see Section 9.2.3). , Zug et al.^^ employed an
intriguing form of back calculation coupled with the Fabens size-based analogue^^
of the von Bertalanffy growth function to generate an apparently age-free estimate
of age-based growth. Substitution of size-based growth parameters, say, from the
Fabens function into the conventional age-based von Bertalanffy function is invalid
(see Section 9.2.4.3).
Klinger and Musick,^^ Zug et al.,^^ and Parham and Zug^^ found significant
growth variability, which is attributable to natural variability in growth rates due to
age, year, and cohort effects. However, they also used growth record discarding so
sampling was explicit mixed cross-sectional confounding age and cohort effects.
The irony is that using back calculation properly (see Section 9.2.3.3) they would
have had mixed longitudinal sampling rather than cross sectional. Skeletochrono
logical age estimation coupled with length back calculation provides the best oppor
tunity for undertaking age, year, and cohort analysis of growth rates (see Sections
9.2.1.2, and 9.3.2.3.4), which could lead to new insights into sea turtle growth
dynamics.
Many sea turtle growth studies have been based on known-age, captive-reared turtles
(Table 9.1). Such studies involve longitudinal sampling, which confounds age and
year effects on growth rates. This is not a major issue if the year effect is irrelevant,
say, in a controlled experiment to study dietary effects on g r o w t h . D u e to space
constraints we will not consider the large number of these sorts of captivity-based
growth experiments, but foreshadow that a critical review is wan'anted. A number
of compilations of captive-reared growth information are a v a i l a b l e . T h e main
insight is that all species develop much faster under various captive conditions than
is apparent in wild stocks. It is also apparent that growth variability of a study cohort
increases with age even under captive conditions, suggesting significant individual
variability in age to sexual maturity.
Several studies^^’^^’^^ fitted parametric growth functions to estimate growth rates
and mean age to maturity. Marquez^^ fitted a von Bertalanffy function to Kemp’s
ridley growth data, implying monotone decreasing size-specific growth rates. On
the other hand, Caillouet et al.^^ used a Gompertz model for Kemp’s ridley, implying
nonmonotone size-specific growth rates. The Gompertz function has excellent sta
tistical properties and the von Bertalanffy function has poor properties.^^ Recall that
Zug et al.^^ also fitted a von Bertalanffy function to estimated age at size data for
wild stock Kemp’s ridleys. All three studies^^’^^’^^ fitted functions to incomplete
ontogenetic ranges. Whether or not this species has nonmonotone size- or age-
specific growth is unresolved although Chaloupka and Zug^^^ have proposed a new
polyphasic age-specific growth model for the Kemp’s ridley based on skeletochro-
250 The Biology of Sea Turtles
functions have poor statistical properties'^ (see Section 9.2.4.2) and, moreover, both
functions were fitted to limited data ranges.
Ehrhardt and Witham^^ made no mention of tagging nor provided standard errors
for fitted parameters, making it difficult to assess data reliability or model adequacy.
Irrespective of these concerns, the empirical growth pattern for the ontogenentic
range observed in their study was asymmetric, implying nonmonotone size- and
age-specific growth rate functions. Ehrhardt and Witham^* provided a time derivative
of the estimated size-at-age function to derive the age-specific growth rate function.
The age-specific growth rate function for this headstarted green turtle stock displayed
increasing growth from hatching to about 10 years of age, then slowly decreasing
to sexual maturity. Ehrhardt and Witham^* used the Chapman-Richards (generalized
von Bertalanffy) growth function, which is asymmetric about the inflexion rather
than skew-symmetric, such as the logistic. If an asymmetric function is needed, then
the Gompertz or an expected-value reparameterization of the Chapman-Richards
function^^ might be appropriate. The Weibull-type growth function used by Cha-
loupka and Limpus^^ (see Section 9.3.2.3.4) is also a flexible asymmetric parametric
function with good statistical properties.
The value of headstarted growth studies is questionable, since growth rates were
accelerated at a critical ontogenetic phase with unknown consequences, an issue
acknowledged by Caillouet et al.^^ Nonetheless, headstarted studies are more infor
mative than captive-reared studies and cannot be discounted until a wide range of
reliable wild stock studies have been earned out — which is not the case at present.
9.3.1.3.2 Known age (wild stock)
Since 1976 around 130,000 loggerhead and 109,000 green turtle hatchlings have
been marked with a mutilation tagging protocol in the sGBR r egi on. One immature
female loggerhead (15.2 years, 75.6 cm ccl), one immature female green turtle (8.75
years, 54.4 cm ccl), and one immature green turtle male (11.25 years, 68 cm ccl)
have now been recovered in a warm temperate foraging area well south of the sGBR
rookeries.^ If tag recognition is reliable, these data are invaluable, but insufficient
at this stage to be of major use for modeling growth dynamics. Other long-term
tagging and monitoring programs will undoubtedly also begin soon to provide known
age wild stock recoveries.
The 21 studies classified as size-focused in Table 9.1 used either known age turtles
(captive-reared, headstarted) or turtles of unknown age. In most cases the purpose
of the study was to investigate growth rates, although many also had the explicit
intention of determining a size-at-age function from length data. All three sampling
designs (cross-sectional, longitudinal, mixed longitudinal) were used, reflecting the
diversity of study settings and research purposes. Only three studies (Bolten et al.,^^
Chaloupka and Limpus,^^ and Chaloupka and Limpus have taken a statistical
approach to better understand growth dynamics. Two studies (Chaloupka and
Linipus^^ and Chaloupka and Limpus'^)have attempted to take into account the
confounded time effects of time-sampled growth information.
252 The Bloiogy of Sea Turtles
Swingle et aU^^' provide weekly growth records for twelve conspecific captive-reared
loggerheads monitored for two years at several different sites. These data reflect
longitudinal sampling, hence age and year effects are confounded, making conclu
sions about wild stocks questionable. Age was known, but the focus was growth
rates rather than age. The weekly growth profiles enable investigation of potential
seasonal growth and perhaps polyphasic growth similar to the study by Phasuk and
Rongmuangsart^'^ on captive-reared olive ridleys discussed above (see Section
9.3.1.2).
9 3 .2 3 .2 Growth interval
Besides the captive-reared growth studies, this group reflects the bulk of sea turtle
growth studies. This is not surprising given that many studies were motivated by
the apparent disparity between growth information obtained from captive-reared
turtles and data obtained from wild stock observations.
Sampling design was mixed longitudinal (but age unknown) except for Green,
B o u l o n , and Collazo et al.,^^ where it was explicit mixed cross sectional due to
growth record discarding, thereby confounding age and cohort effects. As already
mentioned, growth record discarding is an unnecessary practice. For all studies the
year and cohort effects are confounded because age was unknown. Most studies
used multiple monel tagging protocols (see Section 9.2.2.2) except for Balazs et
al.^^ (inconel tags), Limpus^® (titanium tags), and Bjorndal et al.^^ (plastic tags).
Most studies only covered a limited ontogenetic range and provided small sample
sizes.
The basic method is the compilation of mark-recapture growth records over
time. Then mean annual absolute growth rates are calculated and grouped into 5-
or 10-cm-size increments referenced either to mean size or initial size at first capture
to indicate size-specific growth — the appropriate size reference metric being mean
size rather than initial size.^^>^^^ The approach is simple and has been the basis for
useful comparative analyses across a broad geographic r a n g e . S e v e r a l
studies^^’^^’^^’^-'’''^^ found nonmonotonic size-specific growth patterns for green turtles,
loggerheads, and hawksbills from several different geographic locations. Nonmono
tonic size-specific growth implies asymmetric (e.g., Gompertz type) or skew-sym
metric (e.g., logistic type) size-at-age functions. Three studies^^’^"^’^^ (but see also
Bjorndal et al.^^ and Boulon and Frazer^^ found monotonie size-specific growth for
green turtles, loggerheads, and hawksbills from western Atlantic-Caribbean loca
tions. Monotonie size-specific growth implies a noninflexion asymptotic size-at-age
function (e.g., von Bertalanffy type).
The growth interval approach is useful, but limited because the grouping arrange
ment precludes application of individual covariate information essential for any
further statistical modeling (cf. Limpus^^ and Chaloupka and Limpus^^% discussed
in Section 9.3.2.3.4 below).
9.3.2.3.3 Size-based growth analogues
Bjorndal and Bolten^^ used the Kaufman^^ size-based analogue of the von Bertalanffy
growth function to study specific growth in an immature green turtle stock in the
Bahamas. The main focus of this study was growth, not age. The sampling design
was mixed longitudinal, but as age was unknown, year and cohort effects are
confounded. They used the Kaufman^^ approach to determine if the size-specific
growth data implied either logistic, von Bertalanffy, or Gompertz type size-at-age
growth — growth was specific, not absolute growth. The Kaufman approach pre
cluded the need to specify unknown growth parameters such as mean adult size.
They found that von Bertalanffy growth behavior best reflected the size-specific
growth pattern for the recorded size range. The empirical size-specific growth func
tion was monotone decreasing for the recorded size range (25 to 70 cm scl). But
Age, Growth, and Population Dynamics 255
the Kaufman analogue is inappropriate for two reasons — (1) it isn’t applicable for
estimating von Bertalanffy parameters anyway (see Kaufman)^^ and (2) replacing
parameters in age-based functions with parameters from size-based analogues is
invalid (see Section 9.2.43), although this latter use was intentionally avoided by
Bjorndal and Bolton.
The Bjorndal and Bolten^^ study has been extended by Bjorndal et al.^^ in a
comparison of sequential length frequency analysis, growth interval method, and
Fabens analogue of the von Bertalanffy growth function (see also Section 9.3.2.1.1).
This time the main focus was age, but the method was still size based. They used
the Fabens analogue to derive a size-at-age function fitted only to the observed size
range. The Fabens size based analogue has known poor statistical properties'"^ and,
moreover, using any size-based analogue to provide age-based parameter estimates
for the von Bertalanffy growth function is invalid (Francis^^ and references therein;
see Section 9.2.4.3). Nonetheless, it appears the empirical size-specific growth
function for this 25 to 70 cm scl green turtle stock^^*^^ does display monotone
decreasing size-specific growth rates unlike, say, for green turtle stocks in the sGBR
region^^’2^‘^ (see Section 9.3.2.3.4).
The main focus of Frazer et al.,"^^ Frazer and Ehrhart,^^ and Boulon and Frazer^^
and was on deriving size-at-age functions for green turtles and loggerheads from
mark-recapture length data. In fact, the growth function derived by Frazer et al."^^
was used to form the age-structure of a loggerhead population modeF"^ (see Section
9.5.2.2). Recall also that Zug et al.^^ used the Fabens analogue to derive a size-at-
age function for age-estimated Kemp’s ridley in a mixed cross-sectional study (see
Section 9.3.1.1.2).
The sampling design for these four studies should be mixed longitudinal, but
because of growth record discarding it was explicit mixed cross-sectional with age
and cohort effects confounded. All four studies fitted von Bertalanffy type growth
functions to a limited ontogenetic range, assuming that the function reflected the
entire unobserved postnatal development period. This is simply not appropriate. All
four studies used the Fabens size-based analogue approach to derive age-based
growth parameters to substitute into the von Bertalanffy age-based growth function.
As already mentioned this approach is invalid (Francis^^ and references therein; see
Section 9.2.4.3). Frazer and Ehrhart’s study^^ was extended by Frazer^^ using the
Fabens analogue to back transform a length frequency distribution for stranded
loggerheads to derive an age frequency distribution (catch curve) for estimating age-
specific survival. The same issues remained unresolved. We discussed elsewhere
(see Section 9.2.4.3) suitable approaches to size-based growth studies and size-based
analogues.
All seven studies discussed concluded that somatic growth
was best expressed as a von Bertalanffy growth function, implying monotonie size-
and age-specific growth rate patterns. Excluding Bjorndal and Bolten,^'^ the remain
ing six studies"^^’^^’^^’^^’^^'^^ concluded that the von Bertalanffy function reflected
growth for the entire postnatal development phase, which was highly questionable
since no study had sufficient data range, and these six studies used inappropriate
size-based analogues to derive such a conclusion.
256 The Biology of Sea Turtles
There are three common schemes for interpreting life history strategies: (1) deter
ministic r-K theory, (2) stochastic bet-hedging theory, or (3) age-specific reproduc
tion axioms focusing on residual reproductive value (see S t e a ms ) . T h e schemes
are all special cases of a more general demographic classification of environmental
Age, Growth, and Population Dynamics 257
TABLE 9.2
Classification Summary of Sea Turtle Population Dynamics Literature
Examples
/-state Mode Time Focus Ref. (year; no.) Species Modeling approach Location Purpose
Distribution Deterministic Static Age Marquez et al. (1982a; 124) olive Ridley Sequential population analysis Eastern PacificDemographicanalysis
Marquez et al. (1982b; 125) Kemp’s Ridley Sequential population analysis Gulf of MexicoDemographicanalysis
Richardson & Richardson Loggerhead Life table simulation Georgia, U.S. Demographic analysis
(1982; 126)
Frazer (1983, 1984, Loggerhead Life table schedules Georgia, U.S. Demographic analysis
1986; 103-105)
Dynamic Age Doi et al. (1992; 127) Hawksbill General dynamic pool Cuba Policy evaluation
Crowder et al. (1994; 108) Loggerhead BLL matrix projection Southeastern U.S. Policy evaluation
______________ Heppell et al. (1996;24) Loggerhead BLL matrix projection Great Barrier Reef Policy evaluation
Stage Crouse et al. (1987; 106) Loggerhead Lefkovitch matrix projection Southeastern U.S. Policy evaluation
Laurent et al. (1992; 107) Loggerhead Lefkovitch matrix projection Mediterranean Demographic analysis
Crowder et al. (1994; 108) Loggerhead Lefkovitch matrix projection Southeastern U.S. Policy evaluation
Somers (1994; 109) Loggerhead Lefkovitch matrix projection Great Barrier Reef Policy evaluation
Heppell et al. (1996;24) Loggerhead Lefkovitch matrix projection Great Barrier Reef Demographic analysis
Stochastic Dynamic Hybrid Chaloupka & Limpus Green Nonlinear system simulation Great Barrier Reef Policy design/evaluation
IT
(1996;110) CD
CO
Chaloupka & Limpus Loggerhead Nonlinear system simulation Great Barrier Reef Policy evaluation
(1996; 123a) o
Configuration Stochastic Static Age Bustard and Tognetti Green Monte Carlo simulation Great Barrier Reef Demographic analysis
(1969; 137) Ln
CD
Oi
Age, Growth, and Population Dynamics 259
While not stated in van Buskirk and Crowder,^these two groups represent
distinct ontogenetic patterns. Group 1 species live in one general habitat irrespective
of ontogeny — flatbacks remain neritic with no pelagic phased* while leatherbacks
remain pelagic except, of course, when nesting. On the other hand, group 2 species
have a distinct postnatal pelagic phase proceeded years later by recruitment to a
neritic immature adult phase. The transition from pelagic to neritic habitat represents
a major ontogenetic shift and is one of the many reasons why a single growth function
is inappropriate for representing postnatal growth of at least group 2 species, where
growth is clearly polyphasic^^’^^^ (see Section 9.3.1.1.2).
Another possibility regarding these two ontogenetic groups is that they might
well reflect two distinct phyletic groups based on paedomorphic heterochrony (see
Alberch et al.).*®^ Rhodin et al.^^"^ consider that the leatherback skeletal morphology
reflects neoteny, which is pedomorphism due to somatic retardation.N eoteny
results from an ontogeny with a longer exposure to high juvenile growth rates,
leading to a morphologically retarded adult. It is interesting then that leatherbacks
appear to have by far the highest juvenile growth rates of all sea turtle species,
reaching sexual maturity on average in around 13 to 14 years.W hether or not
flatbacks are a macroevolutionary product of neotenic heterochrony like the leath
erback is not known, although flatbacks do retain a number of juvenile features in
adulthood, such as carnivory and poorly keratinized skin and scales.^^’^^^ If they are
neotenic, then we would expect flatbacks to also have higher growth rates, which is
feasible given that they are restricted to a continental shelf habitat with feeding
grounds in shallow, turbid, inshore w a t e r s . I f higher growth rates occur, then
perhaps earlier maturation rates offset the impact of lower flatback fecundity^ on
flatback population dynamics — else lower fecundity could be offset by higher
hatchling and juvenile survival rates, but we just do not know. And therein lies a
consequence of the complex nature of sea turtle life history, growth, and population
dynamics — a litany of unresolved “if-then-else-but” statements.
There are insufficient comparative studies of sea turtle ecology, reproduction,
embryology, morphology, and somatic growth to support further insight into life
history patterns. Comprehensive studies of growth dynamics and population dynam
ics across a range of environmental settings are needed to understand the complex
relationship between sea turtle ontogeny, phytogeny, and macroevolutionary change.
Most sea turtles have an overlapping iteroparous life cycle — long life expectancy
coupled with discrete multiple breeding seasons and overlapping generations. The
nesting season is usually in summer, but a northern Australian flatback stock (N.
depressus) has a winter nesting season.^Several stocks have also been shown to
nest year-round and so have a continuous iteroparous life cycle (e.g., Sarawak green
turtles,Crab Island flatbacks).^Such complexity of life cycles and geographic
variability provides a rich anea for comparative demographic studies.
Comparison of limited growth interval profiles for some stocks indicated inter
regional differences in size-specific growth^^^’^^’^^ and mean adult size^^^ for green
turtles and loggerheads. A difference in benthic phase recruitment size is also
260 The Biology of Sea Turtles
apparent between the Caribbean and the sGBR regions for green turtles, loggerheads,
and hawksbills. In the Caribbean region, greens recruit at 20 to 25 cm scl, logger-
heads at 40 to 45 cm scl, and hawksbills at 15 to 25 cm sclT On the other hand, in
the sGBR region greens recruit at 35 to 40 cm ccl, loggerheads at 68 cm ccl, and
hawksbills at 40 cm ccl.^’^^'^^ Even accounting for tranformation of scl to ccl the
recruitment size differences between regions and between loggerheads and the other
species are considerable. Clearly, there are common and yet local or regional patterns
in sea turtle demography, suggesting that there is sufficient phenotypic variability
to warrant comparative demographic analyses to complement life history analyses.
Zug et al.^^ summarized captive and wild stock growth rates and estimated age
at sexual maturity from many sources, but these data are of limited value for
comparative purposes. Important static s u r v i v a l a n d fecundityschedules have
been derived for a loggerhead nesting stock (Little Cumberland Island, GA). Com
parison of these life schedules with similar schedules derived for other loggerhead
stocks would fomi the basis for a comparative demography, but there seem to be
few age-specific or size-specific survival or fecundity schedules available.
BjomdaE^ presented both cohort and relative age-specific survivorship data for
a green turtle stock nesting at Tortuguero, Costa Rica. Chaloupka^^^'* has used a
statistical model to account for the main effects of age, year, and cohort in a
reanalysis of BjorndaLs^^ data. This modeling approach based on a generalized linear
model with extra-Poisson sampling variation (see McCullagh and Nelder)^^^ provides
an age-specific hazard function conditioned on the time-dependent effects (age, year,
cohort). This time-conditioned hazard function would be suitable for inter-stock
comparison if other such hazard functions were avaliable.
Despite these studies there are no comparative analyses of intra- and interspecific
demography based on key attributes such as age- or size-specific mortality (hazard),
fecundity, somatic growth, or recruitment. It is certainly more difficult to compare
demographic functions than static life history traits based on, say, mean fecundity
or relative reproductive value. Nonetheless, important insights into demography and
population dynamics will arise when such analyses are undertaken. To encourage
such an analysis, which would need to be a collaborative research effort, we outline
a standard modeling procedure that might be suitable for comparative demographic
analysis based on somatic growth dynamics and mortality. Firstly, a statistical anal
ysis of age-specific growth if age known or else size-specific growth rates condi
tioned on time-dependent effects (year, cohort) is needed to estimate the growth
function. If the estimated growth function is size specific, then numerical integration
is needed to transform the function into age-specific form.^^’^^^ Then a suitable
parametric growth function could be fitted to the conditioned size-at-age data derived
from either the age-specific or size-specific forms. The Weibull-type nonlinear equa
tion is useful here for comparative puiposes, especially when used as a system of
nonlinear equations (see Chaloupka and Limpus).^^ The single equation fonn is^^
y = a -- P exp[-exp(-y)fl (9.1)
a = asymptote
p = asymptote - y intercept
Y= growth coefficient
5 = dimensionless shape parameter
If the shape parameter 5 = 1 , then the growth function is an asymptotic regression
function indicating monotone decreasing size-specific growth rates — a von
Bertalanffy type function. The shape parameter enables the equation to fit either
skew-symmetric or asymmetric functions. This model is the only flexible shape
equation with known good statistical properties, and expected-value forms are
avai l abl e. It is easy to derive a size-based analogue of this function, but its
utility is questionable since it is our claim that all time-dependent demographic
data needs to account for the age, year, and cohort effects first.
The statistical estimation Equation (9.1) or a system of equations (9.1) will
include an error term, which can be autoregressive or moving average to account
for the nth order autocorrelation. In a system of equations the parameters can be
either unique to each equation or common across equations, as can the autocorre
lation parameters. The system of nonlinear equations is then best estimated using
nonlinear SUR.^^^
The Weibull-type model would then provide estimates of growth dynamics from
several stocks expressed in summary form as (1) mean mature adult size (a), (2)
growth coefficient (y), and (3) the intrinsic growth function shape parameter (5).
These three parameters coupled with estimated mean age at sexual maturity would
then provide a quantitative basis for inter-stock demographic comparison. These
four key parameters could be derived also as sex-specific functions using the same
process. The Weibull-type modeling procedure presented could also be applied to
survivorship data for inter-stock comparative analysis. The advantage of using one
reliable growth function such as Equation (9.1) for comparing all stocks (sex,
geographic, species) is that it would enable comparison of growth functions among
the stocks in terms of the same growth parameters and estimates of standard errors.
Such a form of comparative demography based on growth and survivorship
would complement well conventional life history analysis such as that of van Buskirk
and Crowder. ^^^The problem with just analysis of life history traits is that phenotypic
variability of traits such as reproductive output is only epigenetic and of low heri-
tability, whereas somatic growth rates, while environmentally influenced, have high
heritability (see Stearns).M oreover, growth dynamics is a fundamental aspect of
macroevolutionary change.Combining comparative demography and comparative
life history would provide better insights into population dynamics. Major challenges
exist in undertaking the modeling procedure related to lack of sufficient time-
structured growth and mortality data sets spanning the entire post-pelagic develop
mental phase.
9.5 P O P U LA T IO N D Y N A M IC S
A population is a set of individuals with a common life cycle, which is a sequence
of morphological states structured by age and/or developmental stages. Population
dynamics is about the analysis of temporal change in abundance and spatial structure
262 The Biology of Sea Turtles
All the deterministic models in Table 9.2^4 11)9 ,124-127 ^^sed on a deterministic
and linear theory of population growth where demographic rates are assumed to be
density independent and stationary (time invariant), resulting in a stable age distri
but i on. I n other words, demographic rates are assumed to be time independent,
thereby confounding age, year, and cohort effects, one implication being that indi
viduals are assumed to develop at the same rate and so reach sexual maturity at the
same age. These assumptions are expressed as knife-edge recruitment in the Marquez
et models.
Age, Growth, and Population Dynamics 263
All models assigned here (Heppell et al.,“"^ Crowder et al.,^°^ Doi et al.)^^^ are some
form of deterministic age-structured matrix model. Doi et al.^^^ used a general form
of dynamic pool or so-called yield-per-recruit stock assessment m o d e l . T h e
dynamic pool model used by Doi et al.*^^ is also a form of generalized Bemardelli-
Lewis-Leslie (BLL) m a t r i x . T h e BLL model is a common mathematical descrip
tion of density-independent growth of an age-structured population observed at
264 The Biology of Sea Turtles
discrete time i nt er val s . The BLL matrix models used by Crowder et al.^^^ and
Heppell et al.^"^ were complements of stage-structured loggerhead models, so we
leave these two studies until the next section.
Doi et al.^^^ developed an extended dynamic pool model to estimate optimal
harvesting yield given hypothetical levels of fishing intensity for a hawksbill fishery
based in Cuba. The extensions included an age-structured cohort, gradual adult
maturation, a piece-wise linear age-specific harvesting risk function and a piece-
wise linear Beverton-Holt-type stock-recruitment function. Fishing mortality and
natural mortality (hazard) were assumed to be continuous and separable functions.
The model makes other major assumptions — (1) growth is well described by a von
Bertalanffy function; (2) a year- and cohort-invariant von Bertalanffy age-length key
to back transform catch-at-length to catch-at-age, since age is unknown; (3) constant
time-invariant recruitment; (4) a constant time-invariant hazard function; and (5) a
constant time-invariant Baranov-type catch function. It is a sophisticated, but
assumption-laden model that is very common in fisheries stock assessment. It incor
porated SPA (see Section 9.5.1.1) to review transient behavior induced by changes
to model parameter inputs. Doi et al.^^^ concluded that the escapement size for
hawksbills in the Cuban fishery would need to be increased to 70 cm scl (12 years
old) to ensure the long-term viability of this stock.
The major concern with this model is that it was based on very little biological
data about the hawksbill stock itself. Doi et al.^^^ relied on captive-reared growth
information (see concerns discussed in Section 9.3.1.2 on captive studies) or theo
retical inputs such as a presumed maximum exposure of immature hawksbills to the
fishery no later than 7 years of age, complete sexual maturation by 14 years of age,
and negligible dispersal to or from the Cuban stock. There were no valid estimates
of age- or size-specific natural mortality, let alone a hazard function conditioned on
age, year, and cohort effects. Since stock abundance in foraging or nesting habitats
was either unknown or poorly known, the model had no empirical stock reference
behavior to assess the heuristic value of the model. Sullivan et al.^"*^ present a general
stochastic size-structured model perhaps more suitable than the extended dynamic
pool model for analysis of sea turtle stock assessment when age is unknown and
the analysis is based on the catch-at-length composition. However, any model will
be of limited value if there are no valid stock-specific demographic data.
The models assigned here (Crouse et al.,^^*" Laurent et al.,^®^ Crowder et al.,^^^
Somers,*®^ Heppell et al.)^"^ are all deterministic stage-structured variants of a general
linear matrix model, which includes the BLL matrix model introduced in Section
9.5.1.2 as a special case.^^i Pnear stage-structured matrix model (Lefkovitch
model) is widely used in ecological m o d e l i n g . N o n l i n e a r extensions*^^ and
linear stochastic analogues of the Lefkovitch model have been proposed.
The main purpose of all these models on loggerheads^"^’*®^'^^"^ was to estimate
stage-specific reproductive value and demographic parameter sensitivity, but increas
ingly they are being also used to simulate temporal stock abundance in response to
demographic perturbations.
Age, Growth, and Population Dynamics 265
Nonetheless, this is a particularly useful simulation because it provided for the first
time important qualitative insights into the long time delays inherent in loggerhead
population recovery following TED introduction. Crowder et al.^^^ used the Crowder
et stage-structured model to forecast population recovery rates using revised
estimates of TED-reduced mortality derived from their recent analysis^"** of logger-
head strandings.
Laurent et al.^^^ developed a stage-structure model for a Mediterranean logger-
head stock using six stages (4 size-based stages and 2 reproductive status stages).
Stage transition (maturation rate) was knife-edge and time-invariant as for similar
Laurent et al.^®^ concluded from a sensitivity analysis (elastic
ity) that reducing annual mortality of loggerheads larger than 70 cm scl (large
juveniles and adults) was essential for stock viability. The model was based on little
empirical demographic information relevant to the stock. There was no empirical
stock reference behavior to assess the heuristic value of this model.
Som ersdeveloped a stage-structured model for the sGBR loggerhead stock.
This model was similar to Crouse et al.,^^^ the main difference being a modified
size-based classification and mainly theoretical demographic parameters reflecting
the stock in a hypothetical reefal setting. Unlike Crouse et al.,'^^ Somersconcluded
that protection of eggs/hatchlings would have a major impact on long-tenn stock
viability. The reason for the difference in conclusion between the two studies was
due to higher egg/hatchling stage mortality rates used in Somers. There was no
empirical stock reference behavior to assess the heuristic value of this model.
Heppell et al.^"^ also developed a stage-structured model for the sGBR loggerhead
stock. This model was similar to Crouse et al.,‘^^ the main difference being a stage
classification for immature and adult loggerheads based on reproductive condition
determined from laparoscopy. The demographic parameters were relevant to a stock
in a hypothetical reefal setting and to a sGBR stock nesting at the adjacent mainland
rookery (Mon Repos). This model used stage-specific survival rates for immature
and mature loggerheads derived from Cormack-Jolly-Seber a n a l y s i s o f capture
profiles derived from a mark-recapture program.^ The survivorship analysis assumed
time-invariant mortality confounding year and cohort effects. Stage durations were
derived from the von Bertalanffy growth function presented in Frazer et al.^^^
Heppell et al.^"^ concluded from sensitivity analysis (elasticity) that reducing
annual mortality of loggerheads larger than about 70 cm ccl was essential for stock
viability. Like Crowder et al.,’^^^ Heppell et al.^“^ then used a BLL matrix form of
their model with 35 age classes to simulate time-dependent responses in adult female
abundance in reply to (1) levels of trawling-related mortality or (2) previous fox
predation of eggs at the mainland rookery. The two mortality sources were not
simulated simultaneously. There were no empirical stock reference behaviors to
assess the heuristic value of these models.
All the stage-structured models discussed in this section assumed time-invariant
and density-independent demographic rates. Therefore, age, year, and cohort effects
are confounded. Individuals in a stage were also assumed indistinguishable with
respect to growth development and time spent in that stage. In other words, all
individuals develop at the same rate and progress to the next stage with the same
maturation rate — similar to the constant knife-edge recruitment assumption of SPA
Age, Growth, and Population Dynamics 267
Bustard and Tognetti*^^ designed probably the first sort of /-state configuration
modeT^^ to investigate sea turtle population dynamics. The model was a Monte Carlo
experiment (see Rubenstein)*^^ to simulate the probability of a green turtle nest being
destroyed as a function of increasing female nesting density. The model was not
well documented, but it seems that hypothetical individual turtles were tracked over
a 35-d nesting cycle as each turtle attempted to nest in a fixed beach area at 15-d
intervals. The model was based on the sGBR green turtle stock nesting on Heron
Island. They used the model to derive a linear function between nesting density and
percentage nest destruction where every 100 additional nesters resulted in an addi
tional 5% nest destruction — all eggs in the nest are destroyed, not a portion.
Bustard and Tognetti^'^^ then proposed that nest destruction was a major density-
dependent mechanism of population regulation. Their linear numerical response
function implies a dome-shaped or extreme Ricker-type^^ stock recruitment function.
There is little basis for adopting either their linear numerical response function nor
the implicit stock-recruitment function. There are no empirical records of extensive
nest destruction for any nesting stock for any species in the Great Barrier Reef
region. Even if such a numerical response function existed it would still be only one
of many factors affecting population dynamics.
9.6 C O N C L U S IO N
Substantial work has been carried out on growth dynamics of sea turtles, increasingly
from wild stocks. Table 9.1 presents many of these growth studies arranged to foster
Age, Growth, and Population Dynamics 269
ACKNOWLEDGMENT
Virginia Institute of Marine Sciences contribution # 2027.
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■j Q Diving Physiology
M olly E. Lutcavage and Peter L. Lutz
CO NTENTS
10.1 INTRODUCTION
Sea turtles are among the longest and deepest diving of the air-breathing vertebrates.
In the wild, most sea turtles spend as little as 3 to 6% of their time at the surface,
where energetic and predation costs may be high, and can thus be considered truly
subaquatic. The central features of their diving ability involve an efficient oxygen
transport system and an extraordinary tolerance of hypoxia which allows maximal
use of limited oxygen stores. Many of the physiological traits that support this breath-
hold mode of life, such as intermittent breathing patterns, adjustable metabolism,
and hypoxia tolerance, are common reptilian features and undoubtedly were present
in the land-dwelling reptilian ancestors of the sea turtle. However, sea turtles have
distinctive modifications in morphology and physiology that allow them to fully
exploit the marine environment in unique ways, and interesting parallels can be
made with the aquatic adaptations shown by marine mammals.^
0-8493-8422-2/97/$0.00+$.50
© 1997 by CRC Press, Inc. 277
278 The Biology of Sea Turtles
For efficient underwater locomotion the sea turtle body plan includes limbs
modified as hydrofoils, a reduction in carapace mass, shortened neck, and body
streamlining. They have nasal passages that close upon diving, orbital salt glands
for salt excretion (see Chapter 14), and some (leatherbacks) can conserve body heat
sufficiently to allow them to forage in cold temperate waters'^ (see Chapter 11).
However, the most important respiratory adaptations for successful breath-hold
diving are those that facilitate efficient and rapid gas exchange when the turtles are
on the surface, and maximize oxygen storage and tissue oxygen delivery while they
are submerged. The important metabolic adaptations that enhance diving prowess
include having an increased anaerobic scope and an enhanced tolerance of hypoxia.
10.2 N A T U R A L D IV E PATTERN S
Sea turtles show a wide range in diving depth and duration, although records for
some species such as the Australian flatback, Natator depressus, are lacking, and
we have no information on adult males of most species (Table 10.1). Deepest dive
depths have been recorded from adult female leatherback turtles, Dermochelys
coriácea (>1000 m),^^^ followed by the olive ridley, Lepidochelys olivácea (290 m,
sex unknown),^^ and adult female loggerheads, Caretta caretta (233 m).^ Although
hawksbills, Eretmochelys imhricata,^^ green, Chelonia mydas,^^ and Kemp’s ridley
turtles, L. kempi,^~^^ tend to remain in shallow water (from 20 to 50 m), a record of
110 m has been given for a green turtle.Hawksbill turtles make the longest routine
dives reported so far (56.1 min, sex unknown),followed by internesting female
olive ridleys (54.3 min),^^ who made longer dives than breeding or post-breeding
males (28.6 and 20.5 min, respectively).The longest reported voluntary dives in
sea turtles range from 2 to 5 h.^ In the olive ridley, diving and surface times of turtles
tracked for extended periods with satellite transmitters varied in relation to repro
duction and migration activities.^* Similarly, satellite-tagged juvenile loggerhead and
Kemp’s ridley turtles showed different mean dive depths and surface intervals,
depending on whether they were located in shallow coastal areas, (short surface
intervals) or in deeper, offshore areas (longer surface intervals).^ ***
In most cases, there seems to be little relation between maximum dive depth
and dive duration. For example, a dive to 211 m by a satellite-tracked loggerhead
off Japan required only 10 min,^ while dives by loggerheads in shallow areas of
Chesapeake Bay are considerably longer.**^^ Perhaps surprisingly, the largest and
deepest-diving sea turtle, D. coriácea, appears to have the shortest routine dives (4
to 11 min).*^-2® Possibly the price of having higher body temperatures and metabolic
rates is that oxygen stores are expended more quickly than in smaller, slower-paced
chelonid sea turtles (see Chapter 11).^*
Despite the brevity of their respiratory phases, sea turtles sometimes spend as
much as 19 to 26% of their time at the surface, engaged in surface basking, feeding,
orientation, and mating.^ * T h e wide variation evident both within and across
species reminds us that sea turtle diving and surface habitats are a reflection not
only of their size and physiological attributes, but of their ecology, environment,
and life history.
Diving Physiology 279
TABLE 10.1
Sea Turtle Dive Records from Published Sources
Dive depth (meters) Dive length (min) % Time
Species Maximum Routine Maximum Routine submerged
Loggerhead (Caretta caretta)
fpn-^’^’ 211-233 9-22 17-30
fpn^ 99 13.5-16.6 14.8-20.5
SubadulP'^ 9-22 19-30 80-94
Kemps ridley (Lepidochelys kempi)
SubadulL’^ <50 300 12.7-18.1 96
ipn>« 167 16.7
Pacific ridley (L. olivácea)
fpn“ 290“ .'2 54.3
mb“ 28.6
Green turtle (Cheionia mydas)
714 110
Subadult‘3 <20 66 9-23
Hawksbill (Eretmochelys imhricata)
inf, d 15 73.5 56.1
Leatherback (Dermochelys coriacea)
>1000 37 4-11
fpn“ 475 50-84 37.4 10-14.5
fpn*^ 2-11
Subadult^^^ 7.7 74-91
Note: Abbreviations: fpn, post-nesting female; inf, internesting female; mb, breeding male; d, daytime;
? not given.
increased from 1.0 to 3.3 breaths between the egg deposition and sand
throwing p h a s e s , a n d the ventilatory frequency increased sevenfold over resting
values when juvenile greens swam at 0.4 m sec“^in a swimming tunnel.^^ Breathing
hypoxic or hypercapnic-inspired gases caused a shift in breathing patterns in both
green and loggerhead turtles, going from intermittent single breaths to clusters of
multiple breaths, shortened breath holds, and eventually, nearly continuous breath-
i n g .2 6 ,29-32
Sea turtle tidal volumes are highly variable, and are strongly influenced by the
physical conditions the measurements are made under, i.e., whether the animal is
swimming in water, held on land, or placed in a supine position.^'^’^^ Values range
from 4 to 14 ml kg^^ in D. coriacea,^^ 33 to 49 ml kg"^ in Caretta and
24 to 187 ml kg~^ in Chelonia mydas, which represent 27% to over 80% of the
total lung volumes for these species. These tidal volumes are much greater than
those found in other reptiles (e.g., Trachemys scripta, 6.9 ml kg'^^^ Crocodylus
niloticus, 11.0 ml kg~0^^ and allow the sea turtle nearly complete exchange of lung
gases in a few respiratory bouts. Most marine mammals also have much greater tidal
volumes than their terrestrial relatives.
respiratory pump capacity beyond that of the passive elastic properties of the lung
itself. The ventilation costs associated with large tidal volumes are further offset by
the compliant lung and potential energy stored in the elastic recoil properties of lung
tissue.^'^’'^^
of the body.^^ Nesting leatherback turtles sometimes produce quite audible growling
sounds, but the role of the respiratory system in vocalization is not well documented
in the sea turtles.
blood and tissue stores for oxygen and accordingly have a different set of adaptations.
For example, in the loggerhead sea turtle some 72% of stored oxygen is carried in
the lungs and tissue oxygen stores are of minor importance.^^ On the other hand,
the deep-diving leatherback turtles have a distinctly different oxygen store strategy
compared to chelonid sea turtles.^^ ^? hematocrit, hemobglobin, and myoglobin
concentrations of the leatherback are among the highest recorded for reptiles, and
approach levels found in diving mammals. The blood and tissue oxygen store of the
leatherback (15.2 ml kg“0 is larger than that of the lung (12.2 ml k g w h e r e a s in
other sea turtles the lung store is larger by at least a factor of two (Figure 10.1).^^’^^’^^
Female leatherback turtles routinely dive to depths that would result in complete
lung collapse, whereas in shallow-diving turtles the lung would remain partially
inflated throughout the dive.^^*^^ However, despite differences in distribution, total
oxygen stores calculated for loggerhead turtles (2 2 . 2 ml O2 kg"^ and the leather
back (27.4 ml O2 kg~0^^ are surprisingly similar. Based on oxygen consumption
measurements and estimates of total oxygen stores, aerobic dive limits of 33 and
70 min have been calculated for a 20-kg loggerhead^^ and the adult leatherback,^^
respectively, encompassing the ranges of dive duration seen in nature.
eora
FIGURE 10.1 Oxygen stores in diving reptiles are divided between the lung, blood, and tissues.
OD
286 The Biology of Sea Turtles
the dive, as the problems of blood oxygen transport are quite different in either
For example, marine mammals and the leatherback sea turtle that store
oxygen in the blood often have comparatively high hematocrits, which increase the
blood oxygen-carrying capacities (Table 10.2). The deep-diving leatherback, for
example, has the greatest hematocrit values of any reptile (32 to 38%).^^ But elevated
hematocrits cause increased blood viscosity, which can make lung to tissue oxygen
transport energetically much more expensive.^ For both adult and hatchling green
turtles. Wells and Baldwin^^ found an exponential increase in blood viscosity at
hematocrits over 30%, and calculated that the optimal hematocrit for this species is
around 30%.
TABLE 10.2
Blood Oxygen Affinity and Transport Properties of Diving Reptiles and
Mammals
Leather- Logger- Green Killer- Weddell
back^^ head^^ turtle^^ Crocodile^'^ whale^^ seap2
P50 is the PO2 at 50% saturation. Hill number is the slope of the line describing the relationship
N o te :
between blood saturation log (S/100 - S) and partial pressure of oxygen, log PO2.
By contrast, for animals that use the lung as an oxygen store, the blood must
continue to pick up oxygen as the dive progresses, in face of declining lung PO2
and blood pH. Here, a large Bohr effect would be disadvantageous for oxygen
binding, since the lung PO2 is falling concurrently with pH. Loggerhead and green
sea turtles not only have Bohr values at the low end of the reptilian range, but, more
significantly, the Bohr effects decline substantially at low saturation levels, i.e., at
the operating region towards the end of a dive when the lung pH and blood PO2 are
lowest.^'^ The shapes of the oxygen binding curves in both species are also favorable
Diving Physiology 287
for oxygen extraction from the lungs during a dive, in that they are steep rather than
sigmoidal at low saturation levels (Hill n coefficient approaches 1).33,65 indeed, the
kinetics of the oxygen-hemoglobin interaction in the green sea turtle would appear
to favor oxygen uptake rather than oxygen release.^^ These adaptations allow oxygen
to be stripped from the lung in the sea turtle to almost below detectable limits.^^’^'^’^^
Interestingly, the variation in blood oxygen affinities between different species
is very wide (Figure 10.2). The highest affinity is seen by the green turtle (Pso= 27
Torr PO2 at pH 7.4),^^ the leatherback turtle has a P5Qof 40 Toit PO2 at PCO2 of
4 . 8 % , and the loggerhead turtle has the lowest affinity reported (PO2 = 49 Torr at
pH 7 .4 ).33,65,79 adaptive significance of such a wide range on oxygen affinities
is not at all clear, since all animals including the leatherback have basically the same
mode of living, i.e., breath-hold diving. By contrast to greens and loggerheads, the
slope of the oxygen dissociation curve of the leatherback at low saturation is shal-
low.27
FIGURE 10.2 Comparison of blood oxygen binding curves in the (b) leatherback (25°C,
pH = 7.52, 4.8% € 0 2 ),^* and in the green (a) and loggerhead (c) sea turtles (25"^C, pH = 7.45,
PCO2 = 37 Torr).^^ The dotted lines indicate position of P50.
It is most likely that the rapidity and extent of the internal changes that occur
during forced or emergency dives are functions of the intensity of underwater
struggling activity as well as the length of submergence. For example, oxygen stores
were depleted within 15 min in tethered green sea turtles diving to escape.^"^ Under
such circumstances survival could be compromised if the animal remains trapped
underwater, caught up in gear such as a shrimp trawl. Indeed, accidental drowning
in shrimp trawls, driftnets, longlines, and other fishing gear has been identified as
the most important source of turtle mortality in the U.S.*^^ In a field study examining
the effects of shrimp trawl tow times on sea turtle survival, there was a strong positive
correlation between tow time and sea turtle deaths.^^ There is also evidence that
trawl capture can cause a rapid and severe disturbance in acid-base balance. Juvenile
Kemp’s ridley turtles subjected to experimental trawls lasting a maximum of only
7.3 min (27°C) showed a marked metabolic acidosis (a sixfold lactate increase from
pretrawl conditions) and increased breathing frequency nearly tenfold upon emer
gence.^^ Loggerhead turtles captured in shrimp trawls with tow times of less than
30 min showed severe metabolic acidosis with blood lactate values ranging from
8.8 to 16.2 Lactate recovery times were long, taking as much as 20 h in
loggerheads and Kemp’s ridleys, indicating that turtles are probably more susceptible
to lethal metabolic acidosis if they experience multiple captures, because they would
not have had time to process lactacid loads.^^’^^’^^
Additional factors such as size, activity, water temperature, and interspecific
differences also bear directly on metabolic rates and aerobic dive limits and will
also influence trawl endurance times.^'’ For example, larger sea turtles are capable
of longer voluntary dives than small turtles. Juvenile sea turtles therefore may be
more vulnerable to the stress of enforced submergence than adults, especially during
the warmer months when routine metabolic rates are higher.^^ Disease factors and
hormonal status may also play a role in anoxic survival in forced submergence. For
instance, green turtles afflicted with fibropapillomas or spirorchidaisis may be espe
cially vulnerable to trawl stress. Hematocrits as low as 25% of normal values occur
in afflicted turtles, which would cause a reduction in the blood oxygen transport
capacity. In freshwater turtles, thyroid hormones appear to have a role in setting
metabolic rate, suggesting that hormones may also influence the metabolic status of
a sea turtle.^^
10.6 A N O X IC T O L E R A N C E A N D H IB E R N A T IO N
Characteristically, the vertebrate brain has an absolute dependence on oxygen and
dies with a few minutes of its absence. This is true of the marine mammal, and
maintaining an adequate supply of oxygen to the brain is probably the ultimate
determinant of dive endurance. However, the brains of a few species of freshwater
turtle, and at least the loggerhead sea turtle, have the extraordinary ability to survive
many hours of anoxia, indicating that these species can remain underwater without
breathing for greatly extended periods.
The mechanisms that protect their brains from anoxic failure have been the
subject of several recent r e v i e w s . I n essence, the anoxic turtle brain is able to
maintain ATP levels and ionic homeostasis by severely reducing its metabolic
290 The Biology of Sea Turtles
demands to a level that can be fully met by anaerobic glycolysis. Factors involved
include an increase in the concentration and release of inhibitory neurotransmitters
such as gamma aminobutyric acid and the cofactors such as adenosine and gamma
butyric acid, and a reduction in the concentration of excitatory neurotransmitters
such as glutamate.^^’^"^
The ability to tolerate complete anoxia may allow some freshwater turtle species
such as Chrysemys pietà to hibernate in frozen-over anoxic freshwater pools.'^^ For
sea turtles its use is not so clear. There are a few reports of possible hibernation in
sea turtles. Felger et al.^^ described an assemblage of overwintering green turtles,
Chelonia agassizi, in the Gulf of California, partially buried in the sandy bottom
and believed to have been resident from 1 to 3 months (presumably without eating
or breathing) at water temperatures below 15°C.^^ Torpid sea turtles have also been
recorded off central Florida and coastal Georgia. In the winter of 1977 to 1978 large
numbers of loggerheads were caught by trawling in the Cape Canaveral ship chan
nel These animals were stained black by the anoxic sediments, suggesting that
they had been lodged in the channel mud for a long period. Laboratory studies also
indicate a dramatic reduction in activity in seawater temperatures below 11 to
15"^C.^^’^^ However, it has not been established that cold ocean waters actually cause
sea turtles to become apneic and completely inactive for days. Indeed, there is
evidence from satellite tracking studies that migrating loggerhead and Kemp’s ridleys
remain active below 10°C and as low as 6°C.^ Loggerhead turtles subjected to
seasonal low water temperatures off Tunisia reduce activity, but continue to forage.^^
Apart from incidences of cold stunning of young juveniles in New England waters,
it is likely that as winter approaches most sea turtles living in temperate waters start
to migrate towards warmer tropical waters and do not experience severely cold
temperatures.
10.7 C O N C L U S IO N S
From recent studies we have started to gain an appreciation as to how sea turtles
and other divers manage breath-hold dives. This involves fairly radical adaptations
in the lung, blood, and tissue compared to other reptiles. Interesting parallels between
marine mammals and sea turtles in respiratory system structure and in deep diving
vs. shallow diving strategy provide fascinating examples of convergent evolution.
Yet much remains to be learned about the integration of physiological and cognitive
responses to diving. For example, heart rate, pulmonary, and peripheral circulation
may change abruptly in anticipation of surfacing or diving, and also change with
activity during a dive, suggesting that the central nervous system coordinates and
modulates the dive r e s p o n s e . T h e details of these responses in free-diving
animals remain elusive.
We most especially need to know the characteristics and scope of the physio
logical responses of the sea turtle in the field. Huge gaps in our knowledge include
how natural respiratory and cardiovascular patterns and alterations in blood gases
relate to underwater activity, and how these variables change in relation to diurnal
activity and season. Very recently, newly developed physiological sensors and
Diving Physiology 291
miniaturized computers that record heart rate, swim velocity, and blood flow infor
mation on free-ranging pinnipeds and diving birds have been deployed on sea turtles.
Satellite telemetry has also opened new avenues of research on the pelagic behavior
of sea turtles, but how the different sea turtle species and their diving habits are
affected by environmental changes such as cold snaps is poorly documented. Finally,
it is a goal of sea turtle conservation efforts worldwide to reduce sea turtle deaths
in fishing gear, identified as the greatest single threat to their survival (see Chapter
13). We must then fully understand how enforced submergence produces lethal stress
in animals better known for their anoxic tolerance and durability.
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Diving Physiology 295
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296 The Biology of Sea Turtles
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1 "I Thermal Biology
James R. Spotila, Michael P. O'Connor,
and Frank V. Paladino
CO NTENTS
11.1 IN T R O D U C T IO N
The biology of sea turtles is intimately tied to the thermal constraints of their
environment. From the time a hatchling enters the water, a sea turtle lives in an
environment that is thermally stable in any given place, but that can vary in tem
perature through time, with depth, and with geographic location. In general, it is
only the female sea turtle that comes onto land and only when she is nesting.
Nevertheless, temperature plays an important role in the biology of the nesting
process as well. Sea turtles are unusual among reptiles because their large body size
allows adults to use insulation and blood flow to alter or control body temperature
(Tb). Therefore, there is a large difference in the ability of hatchlings, juveniles, and
adults to thermoregulate actively.
The environmental constraints on the thermal energetics of sea turtles were
reviewed by Spotila and Standora,‘ and recent advances were reviewed by Spotila.^
Considerable progress has been made in the last 10 years in determining the ther
moregulatory mechanisms of sea turtles. This has been accomplished by laboratory
studies and by the application of sophisticated physiological techniques to field
conditions. In this chapter we will review the basic biophysical constraints on the
thermal biology of sea turtles, the themioregulation of sea turtles on land and in the
water, and the role of body size and metabolism in thermoregulation. For a review
of early research on the thermal biology of sea turtles see Mrosovsky.^
0-8493-8422-2/97/$0.00+$ 50
© 1997 by CRC Press, Inc, 297
298 The Biology of Sea Turtles
Conduction-convection
FIGURE 11.1 Heat exchange between sea turtles and their environment. On land, heat
transfer occurs via radiation, convection (wind), evaporation, and conduction. In water, heat
transfer occurs by conduction-convection. (From Spotila, J. R. and Standora, E. A.,Copeia,
1985, 694, 1985. With permission.)
Q abs + M - R + C + E + G ( 11 . 1)
Thermal Biology 299
where:
= radiation absorbed by the surface of the animal from the sun (solar radiation)
and the surroundings (thermal radiation) (W m“^)
M = metabolic heat production (W m“^)
R = thermal radiation emitted by the surface of the animal (W m“^)
C = heat energy lost by convection (W m'^)
E = heat energy lost by evaporation of water or heat energy gained by condensation
of water (W m~^)
G = heat energy lost or gained by conduction through direct physical contact of
the animal with soil, water, or substrate (W
Using this equation we can predict the of a plant or animal if we know its heat
transfer properties and the thermal energy environment to which it is exposed. Details
of the mechanisms of heat transfer in animals are presented by Spotila et al.^ and
do not need to be repeated here.
Porter and Gates'^ apply this approach to animals to produce climate space
diagrams. These diagrams give thermal limits for birds, mammals, and reptiles.
While useful for small animals, climate space diagrams are less useful for large
reptiles because large crocodilians and sea turtles have high internal heat storage
capacities and therefore large thermal inertia.^’^^Therefore, climate space diagrams
indicate long-term or average limits on the thermal biology of these large animals.
Of more interest for sea turtles are transient energy budgets. The reason for this is
that we need to predict body temperature of a sea turtle through time in order to
understand its thermal biology.
Equilibrium and transient energy budget equations make different assumptions,
are solved by different methods, and provide answers to different questions. Equi
librium equations provide information about the thermal environment of the animal.
The Tb computed from Equation 11.1 is the T^ that a sea turtle would come to if
the turtle stayed in one place for a long time and neither the environment nor the
turtle changed. Since most sea turtles have large thermal inertia and, thus, seldom
equilibrate with environmental conditions, their T^, tends to average out the changing
environmental temperature over several hours or days. As the thermal environment
changes, the turtle T^ is also changing (Figure 11.2). It approaches the environmental
temperature with a lag due to its large heat capacity. Environmental temperature
reaches a peak and starts to drop while the body core of the sea turtle is still warming
up. Body temperature reaches a peak while environmental temperature is dropping,
and then follows it down. In essence, the T^ of the turtle is chasing the effective
temperature of the environment. Even so, the turtle should respond behaviorally to
a changing thermal environment because it senses changes in skin temperature. The
turtle has to anticipate changes in environmental heat load before they occur to avoid
overheating due to its own internal heat transfer lag. A good example of this problem
is reported by Colbert et al.,^* who found that when they removed an alligator from
the sun as its T^ approached the lethal value, T^ continued to rise for some time,
and the alligator died of heat stress despite being in the shade for hours. Thus, we
300 The Biology of Sea Turtles
Time
FIGURE 11.2 An idealized relationship of body temperature (T^,) change to environmental
temperature change in a variable environment. Environmental temperature is the operative
environmental temperature (see text). In a large animal such as a sea turtle, the core
never comes to equilibrium with the % if that temperature fluctuates rapidly. The is
moderated or dampened and changes more slowly and less dramatically than the It appears
as if the chases, but never catches the T^. Thus, a large sea turtle has a more constant
temperature than its environment because of its thermal inertia. (From Spotila, J. R., O’Con
nor, M. R, Dodson, R, and Raladino, F. W.,Mod. GeoL, 16, 203, 1991. With permission.)
need to have a means to study the instantaneous response of sea turtles to their
thermal environment and we need to have a means to study transient heat transfer,
because this determines at any given time.
We can study the equilibrium and instantaneous energy exchange of a sea turtle
by making use of the concept of operative environmental temperature (TJ as defined
by Bakken and Gates,Bakken,*^ and Bakken et al.^"^ The Tg is a temperature that
indicates the heat load that the environment places on the surface of an animal. It
averages the effect of solar radiation, heat radiation from the suiToundings, and
convection into a single temperature value. This is the temperature of a darkened
environmental chamber with black walls that would provide the same thermal envi
ronment (heat load) as that experienced by the animal under the conditions in which
the Tg is being measured. The Tg can be computed from Equation 11.1 or can be
measured with an operative temperature model that has the same size, shape, and
radiative properties as the real animal in question. Thus, Standora et al.^^ used a
large hollow copper model of a sea turtle to measure the Tg of green turtles. Cheionia
mydas, on the beach at Tortuguero, Costa Rica. The use of such a model would
allow a detailed study of phenomena such as the basking of green turtles at French
Frigate Shoals in the North Pacific. Caution must be applied with this technique,
however, because it is not a “cure all” for all biophysical studies of animals. Many
poorly conceived and executed studies have produced unclear data because the
investigators did not take the time to understand the intricacies of the Tg concept.
Thermal Biology 301
Bakken^^ provided a summary and critical review of the uses and measurements of
Tg. We do not repeat that analysis here. Potential users of this technique would be
well advised to consult this and other papers by Bakken to develop clearly their
question and methodology before carrying out a study of sea turtles.
Most importantly, before using a model one must understand that such models
(particularly for large animals) have several limitations. (1) Models must have the
same heat exchange properties (radiation, convection) as the real animal. It is not
sufficient to take a piece of copper pipe or a large sphere and paint it some color
that appears to look like a sea turtle, and then assume that the model represents the
turtle. The visual color may have little relevance to the absorptivity of the surface
to visible and near-infrared solar radiation, because the near infrared is a large
component of the total absorbed radiation, and our eyes cannot see it. (2) The T^
models do not account for metabolism and do not model evaporation effects very
well, unless you cover the model with a wet surface, but that would change the
radiative properties as well. Of course, sea turtles do not have wet skins, so that is
less of a problem than for amphibians. (3) Models must be assessed in the same
thermal environment as that experienced by the animal. For example, a sea turtle
model needs to be in the same position as the sea turtle on the beach. If it is a green
turtle model for a nesting turtle, then it needs to be in a body pit. (4) A model of a
large animal such as a sea turtle may have problems with gradients among its parts.
That is, the top surface (carapace) will be hotter than the true T^ of the animal, while
one of the limbs will be another temperature, etc. The gradients in Tg may be real
or may be an artifact of the model. It is possible that the Tg of the carapace is different
than the real Tg of the plastron or front limb because those parts of the turtle
experience different heat loads, or those differences may be due to a problem of air
circulation in the model. That would have to be determined by more complicated
modeling and computational efforts. In using a Tg model of an animal, we are looking
for the average Tg of the whole animal. Therefore, the true Tg will be best estimated
by the internal air temperature of the model. If the regional differences in Tg are
very troublesome, it may be necessary to put a small fan into the model to assure
good air circulation. (5) By design, a Tg model is not supposed to measure T^. It is
designed to measure the heat load on the surface of the animal. That is why we do
not fill it with water or some other substance. We do not want it to respond with
the same time constant as the real animal. We are measuring the heat exchange at
the surface, not the T^ of the animal.
Transient energy budget equations predict how the T^ of an animal will change
with time. If the animal is already at its equilibrium T^ and the environment is stable,
no change in T^ is predicted. Thus, the equilibrium energy budget equation is a
special case of the transient energy budget equation with the heat storage term = 0.
The transient model is more generalized. While the equilibrium equation can be
solved as an algebraic equation, the transient model involves the solution of a
differential equation. There are standard solutions for transient energy budget equa
tions,^’ and transient models have proven useful for consideration of heat transfer
in large reptiles, including sea turtles^^^ and d i n o s a u r s . T h e basic conclusion of
these studies is that as body size of an animal increases, fluctuations in core T^, are
reduced on hourly to daily time scales. For example, calculations by Spotila et al.^^
302 The Biology of Sea Turtles
indicate that the of the dinosaur Compsognathus (1.96 kg) would vary with
fluctuations in Tg of a few hours, like a large green iguana, while Tj, of Deinonychus
(75 kg) would vary with fluctuations on the order of less than a day, like an olive
ridley turtle (Lepidochelys oliváceo) on land, and Ti, of Tenontosaurus (624 kg)
would vary with % changes on the order of a week, like a leatherback turtle
{Dermochelys coriáceo) on land (Figure 11.3). Of course, sea turtles in water would
be exposed to more rapid heat transfer at the surface of the skin (see below). Thus,
differences in body size alone, both within and between species, result in different
thermoregulatory problems for sea turtles. Sea turtles can only thermoregulate within
the constraints placed upon them by the interaction of their physiological, behavioral,
and physical characteristics and the physical characteristics of their environment.
Within those constraints biological interactions, for example, predation by sharks,
will further limit the ability of sea turtles to thermoregulate.
FIGURE 11.3 Predicted responses of dinosaur body temperatures (T^) to variations in the
thermal environment. Fractional amplitudes predicted for various-sized dinosaurs when
exposed to fluctuations in the thermal environment with periods ranging from 1 min to l(f
min (approximately two years). Fractional amplitudes are changes in \ expressed as a fraction
of the total difference in between one equilibrium and another. Thus, a fractional
amplitude of 0.5 indicates that T^ would change half the way from one equilibrium temperature
to another. Different symbols and lines represent different masses. Multiple symbols for a
particular mass and period represent the effect of varying cardiac output from 20% of the
predicted resting flow rate to 10 times the resting rate. Values are presented for 0.2, 0.5, 1.0,
2.0, 5.0, and 10.0 times resting flow rates. Lines go through values for 1.0 times resting flow
rate. In all cases, lower cardiac outputs result in lower predicted fractional amplitudes,
indicating that core T^ would change less in response to changing environmental temperature
with lower rates of blood flow. Increases in body size and decreases in blood flow isolate
core Tb from environmental temperature. (From Spotila, J. R., O’Connor, M. R, Dodson, R,
and Paladino, F. V., Mod. GeoL, 16, 203, 1991. With permission.)
Thermal Biology 303
TABLE 11.1
Time Constants for Cooling Experiments in Green
Turtles in an Environmental Chamber Aboard the Alpha
Helix at Cayos Miskito, Nicaragua in August, 1978
Temperatures (°C)
Turtle mass (kg) Ta Te Tb AT X (min)
The differential effects of body size and wind speed on environmental heat load are
apparent in the for different sized turtles. A 50-kg turtle with little wind would
have a of 58.3°C, while an increase of wind to 5 m s~* would lower to 30.0°C.
At the same wind speeds a 200-kg turtle would be exposed to of 58.7 and 36.0°C.
For an explanation of the relationship between body size, wind speed, and convection
coefficient in sea turtles see Spotila and Standora.^
11.4 BASKING
Sea turtles bask on land and in the water. Terrestrial basking by green turtles occurs
in the northwestern Hawaiian I s l a n d s , i n Australia,^^ and historically in the Gal
apagos Islands and Mexico.^^ Whittow and Balazs^^ completed the most comprehen
sive study of this phenomenon at French Frigate Shoals in the northwestern Hawaiian
Islands. Here turtles come ashore and bask on the side of islands facing the outer
reef and the prevailing northeast trade winds. Sand temperatures and black globe
temperatures (an approximation of were lower on beaches used for basking
than on beaches on the opposite side of the island that were not used by basking
turtles (Figure 11.4). If we use black globe temperature as a measure of Tg, then it
is apparent that beaches used for basking are cooler than nonbasking beaches.
Perhaps turtles would overheat if forced to stay out on the hotter beaches.
Our calculations of heat stress in green turtles (above) suggest that T^ of 40°C
or higher would result in heat stress for this turtle. Carapace temperatures were as
high as 40.0 to 42.8°C in basking turtles. By flipping sand onto their flippers and
carapace, turtles lowered surface temperatures by as much as 10°C. This would
Thermal Biology 305
Time (h)
FIGURE 11.4 Environmental conditions experienced by green turtles basking on French
Frigate Shoals in the Pacific Ocean.^^ Upper lines (triangles) indicate temperatures measured
with a black globe thermometer and lower lines (circles) indicate temperatures of the sand.
Temperatures on the basking beach (solid symbols) are lower than temperatures of the non
basking beach (open symbols). The black globe temperature, which approximates T^, is much
higher than sand temperature on both beaches. (From Spotila, J. R. and Standora, E. A.,
Copeia, 1985, 694, 1985. With permission.)
reduce heat load on the peripheral tissues and allow the turtle to warm up deep
without overheating the peripheral tissues. Basking on land was most prevalent
among female turtles and appeared to be fostered by cool ocean temperatures
(26.3°C) and a combination of white sand beaches with steady wind and moderate
solar intensity. It is possible that females may be able to accelerate development of
eggs by being at a higher T^. Some authors have also suggested that these turtles
are not thermoregulating because they do not use the hottest beach. Instead they
may be avoiding predators such as sharks and avoiding mating encounters with male
turtles. Data available to date are consistent with a themioregulatory interpretation
of this basking behavior. Additional studies are needed to clarify the driving force
behind this behavior. These should include detailed studies of basking behavior of
individual turtles and accurate measurements of the T^ to which these turtles are
being exposed.
306 The Biology of Sea Turtles
FIGURE 11.5 Temperatures of a 110-kg adult female green turtle swimming in the Car
ibbean Sea at Tortuguero, Costa Rica. A multichannel sonic transmitter with remotely posi
tioned thermistors transmitted temperatures from the deep body (triangles), inside the plastron
(squares), carapace surface (open circles), and water (crosses). This female was released from
land at 1100 h, remained a few hundred meters off shore and was relatively inactive.^^ (From
Spotila, J. R. and Standora, E. A., Copeia, 1985, 694, 1985. With pennission.)
0.9°C above and that this difference increased with increasing body mass to a
maximum of 1.7°C (Figure 11.7). These turtles did not so much regulate at a constant
T^, as maintain a relatively constant temperature difference between T^ and T^. They
spent most of their time within 30 m of the surface, and the light intensity data
suggested that they were not basking. Body temperatures were higher than T^ both
on cloudy days and at night. It appeared that the relative constancy of in these
turtles was due in part to thermal inertia as would be expected from biophysical
considerations. A 70-kg loggerhead would have a time constant of perhaps an hour
in water (see above and References 1, 20, 22, and 23) and a resultant overall thermal
lag of a few hours. Thus, variation in T^ would be dampened out and it would remain
somewhat above T^ with less variability as the turtle swam from a water mass at
one temperature into a water mass at another temperature. Thus, as cautioned by
Neill and Stevens,w hile these data indicate that loggerheads enjoy considerable
thermal inertia and have some endothermic capabilities, they do not conclusively
demonstrate that these turtles regulate T^, by physiological means.
Despite the thermoregulatory adaptations of green turtles and loggerheads, nei
ther species functions well at lower T^. This makes them susceptible to cold stunning,
a winter phenomenon in temperate and subtropical waters in which the T^ drops
308 The Biology of Sea Turtles
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quickly (in a few days) and turtles become in cap acitated .K em p ’s ridley, L. kempi,
turtles are similarly affected. Cold stunning occurs when T^ drops below 8°C before
sea turtles can swim away from an area during the sudden onset of a cold front. The
turtles lose their ability to swim and dive, lose control of buoyancy, and float to the
surface. If not rescued and resuscitated by humans, most of these turtles die. The
physiological basis for this response is unknown, but probably involves disruption
of metabolic pathways and disruption of ion gradients across membranes
There remains a need for studies to determine the low-temperature tolerance of
sea turtles, their high-temperature tolerance, and their preferred temperature. These
studies can be most easily done on hatchlings, but are also needed for juveniles,
subadults, and adults. It may be impractical to study large individuals and difficult
to obtain permits to study the thermal tolerances of a sea turtle like the Kemp’s
ridley, which is highly endangered. However, carefully designed and nonlethal
Thermal Biology 309
FIGURE 11.7 The relationship of mean difference between body temperature and water
temperature to body mass of loggerhead turtles swimming in the Pacific Ocean off Japan
during their intemesting periods. Mean values are presented for each turtle. The linear
regression is significant at p = 0.05. (Redrawn from Sato, K., Sakamato, W., Matsuzawa, Y.,
Tanaka, H., Minamikawa, S., and Naito, Y, Mar. Biol., 123, 197, 1995. With permission.)
studies of critical thermal maximum and critical thermal minimum (CTM^^ and
will elucidate thermal limits, and preferred-temperature studies will clarify
the role of temperature in behavior and physiology of these species. These studies
can be done without harming individual turtles and should be done so that we can
understand the thermal biology of these species and avoid loss of sea turtles to
thermal events such as cold stunning in the future.
analyzers (Figure 11.8).^^ Metabolic rates for leatherbacks at rest were above those
predicted by allometric relationships for green turtles and other reptiles scaled to
leatherback size (0.387 W kg“^ 1.15 ml O2 kg~‘ min ^^ but half the values predicted
for mammals of this size. Lutcavage et al."^^ reported similar metabolic rates (1.1 ml
O2 kg”*min”*) for leatherbacks using another method (difference in gas concentration
during rebreathing). It is interesting to note that hatchling leatherbacks also have a
metabolic rate (0.286 1 kg”*h”*= 4.77 ml kg”*min *) three times that of green turtle
and loggerhead hatchlings."*^ Leatherback hatchlings swam continuously in the
respirometer and maintained a high level of activity for hours. They may have a
higher routine (noimal) metabolic rate than the other species, as do the adults.
2.0
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200 250 300 350 400 450
Mass (kg)
FIGURE 11.8 Mass specific metabolic rates for nesting leatherback turtles at Tortuguero,
Costa Rica. We measured metabolic rate for turtles at rest (crosses), while they were covering
their nests (solid squares), and while they were walking on the beach (open circles). We
collected expired air in large Douglas bags and measured the Oj and CO2 content of that air
with a Beckman C-2 gas analyzer and Scholander 0.5-ml gas analyzer. The lowest values of
active metabolism were for turtles when they were covering their nests with their hind flippers
after laying their eggs. The highest values were for turtles vigorously throwing sand with
hind and foreflippers, crawling, and rotating the body from side to side while covering the
nest with sand. (From Paladino, F. V., O’Connor, M. R, and Spotila, J. 344, 858,
1990. With permission.)
temperatures in the North Atlantic and avoid overheating in warni tropical waters.
When leatherbacks are nesting it is typical to observe changes in color of their soft
skin. When they crawl out on the beach their skin is pale. After nesting their skin
is bright pink. We measured blood flow in the skin (unpublished data) and found
that blood flow when the skin appears pink is more than 10 times higher than when
the skin is pale. This increased blood flow helps to cool the turtles by bypassing the
insulation and bringing heat to the surface where it can be transferred to the cool
night air. This strategy would be ineffective during the day when T^ is much higher
(see above). Thus, while the leatherback has a large enough body size to dampen
the effects of solar heating if it nested during the day, the high T^ at its surface would
make thermoregulatory strategy of the leatherback of changing blood flow to the
surface to regulate T^ ineffective. Thus, while it could avoid overheating from
external heat load due to its themial inertia, it could not get rid of the excess heat
generated within its body during the nesting process. Here again, biophysical con
straints would combine with characteristics of the anatomy and physiology of the
sea turtle to detemiine its behavior.
11.7 CONCLUSIONS
The thermal biology of sea turtles is determined by the interaction of the biophysical
constraints imposed by the environment with the anatomical, physiological, and
behavioral characteristics of the animals. We can obtain an understanding of the
thermal constraints on these turtles by considering the limitations imposed by heat
energy exchange with the environment. Judicious use of biophysical models can
help to pose useful questions and to answer them. On land, sea turtles are affected
by rapid heat gain during the day, and leatherbacks are also affected by the inability
to dump metabolic heat during nesting activity. Heat gain and the inability to lose
heat prevent large species of sea turtles from nesting during the heat of the day. In
water, heat gain and loss are very rapid and T^ tend to remain close to T^. Larger
sea turtles such as loggerheads and green turtles can maintain a small (1 to 2°C)
temperature difference between the body core and the water. Green turtles when
swimming vigorously can be as much as 7°C above T^. This endothermic capacity
is even greater in leatherback turtles that can be active in cold northern waters that
have temperatures between 0 and 15°C. They use a combination of large body size,
thick insulation, an elevated metabolism, and changes in blood flow to remain
wanner than the water in cold oceans and to avoid overheating in tropical waters
and on the nesting beach at night. Their strategy of changing blood flow to dump
heat on the nesting beach is ineffective during the heat of the day, because the T^
of their skin is too high to allow heat loss by convection. As we stated in 1985,
additional experiments are still needed to elucidate the thermal biology of sea turtles.
These include such simple measurements as determination of the preferred T^ and
CTMs of these animals, as well as more sophisticated measurements of the thermal
biology of sea turtles free ranging in the ocean. With the increased availability of
sophisticated sensors, recorders, and telemetry, such measurements can now be a
reality.
312 The Biology of Sea Turtles
R EFEREN CES
23. Bakken, G. S., An improved method for determining thermal conductance and equi
librium body temperature with cooling curve experiments,/. Therm. Biol., 1, 169,
1976.
24. Balazs, G. H., Green turtle migrations in the Hawaiian archipelago,^//)/. Conserv.,
9, 125, 1976.
25. Balazs, G. H., Synopsis of biological data on the green turtle in the Hawaiian Islands,
NOAA Tech. Memo. NMFS, NOAA-TM-NMFS-SWFC-7, Honolulu, Hawaii, 1980.
26. Balazs, G. H. and Ross, E., Observations on the basking habit in the captive juvenile
Pacific green turtle, Copeia, 1974, 542, 1974.
27. Bustard, R., Sea Turtles, Natural History and Conservation, Taplinger Publishing,
New York, 1973.
28. Fritts, T. H., Marine turtles of the Galapagos Islands and adjacent areas of the eastern
Pacific on the basis of observations made by J. R. Selvin 1905-1906,/. HerpetoL,
15, 293, 1981.
29. Paladino, F. V., Spotila, J. R., O’Connor, M. R, and Gatten, R. E., Jr., Respiratory
physiology of leatherback turtles {Dermochelys coriacea) while resting, laying eggs,
and active on land, Chelonian Conservation and Biology, in press.
30. Prange, H. D., Energetics of swimming of a sea turtle,/. Exp. Biol., 64, 1, 1976.
31. Prange, H. D. and Jackson, D. C., Ventilation, gas exchange and metabolic scaling
of a sea turtle, Respir. Physiol., 27, 369, 1976.
32. Jackson, D. C. and Prange, H. D., Ventilation and gas exchange during rest and
exercise in adult green sea turtles,/. Comp. Physiol., 134, 315, 1979.
33. Naito, Y., Sakamoto, W., Uchida, L, Kureha, K., and Ebisawa, T., Estimation of
migration route of the loggerhead turtle Caretta caretta around the nesting ground,
Nippon Suisan Gakkaishi, 56, 255, 1990.
34. Sakamoto, W., Uchida, I., Naito, Y, Kureha, K., Tujimura, M., and Sato, K., Deep
diving behavior of the loggerhead turtle near the frontal zone, Nippon Suisan Gak
kaishi, 56, 1435, 1990.
35. Sakamoto, W., Naito, Y, and Uchida, I., Measurement of the migration route of the
loggerhead turtle by Argos and a biorecorder Biotelemetry, XI, 112, 1990.
36. Sakamoto, W. and Yuzuriha, H., Tracking free-swimming marine animals, S£?a Tech-
noL, 34, 25, 1993.
37. Sato, K., Sakamoto, W., Matsuzawa, Y, Tanaka, H., Minamikawa, S., and Naito, Y,
Body temperature independence of solar radiation in free-ranging loggerhead turtles,
Caretta caretta, during intemesting periods. Mar. B iol, 123, 197, 1995.
38 Sapsford, C. W. and van der Riet, M., Uptake of solar radiation by the sea turtle,
Caretta caretta, during voluntary surface basking. Comp. Biochem. Physiol., 63A,
471, 1979.
39. Neill, W. H. and Stevens, E. D., Thennal inertia versus thermoregulation in “warm”
turtles and tunas, Science, 184, 1008, 1974.
40 Witherington, B. E. and Ehrhart, L. M., Hypothermic stunning and mortality of marine
turtles in the Indian River Lagoon system, Florida, Copeia, 1989, 696, 1989.
41. Morreale, S. J., Meylan, A. B., Sadove, S. S., and Standora, E. A., Annual occurrence
and winter mortality of marine turtles in New York waters,/. HerpetoL, 26, 301,1992.
42, Hochachka, P. W. and Dunn, J. F, Protecting cells and tissues against hypoxia and
hypothermia, in Hypoxia and Cold, Sutton, J. R., Houston, C. S., and Coates, G.,
Eds., Praeger, New York, 1987, 57.
43 . Hutchison, V. H. and Dupre, Thermoregulation, in Environmental Physiology o f the
Amphibians, Feder, M. E. and Burggren, W. W., Eds., University of Chicago Press,
Chicago, 1992, 206.
314 The Biology of Sea Turtles
44. Goff, G. P. and Lien, J., Atlantic leatherback iuxiX^^.Dermochelys coriacea, in cold
water off Newfoundland and Labrador, Can. Field Nat., 102, 1, 1988.
45. Frair, W., Ackman, R. G., and Mrosovsky, N., Body temperature of Derrnochelys
coriacea: warm turtle from cold water, Science, 177, 791, 1972.
46. Lutcavage, M. E., Bushnell, P. G., and Jones, D. R., Oxygen stores and aerobic
metabolism in the leatherback sea turtle, Can. J. Zool., 70, 348, 1992.
47. Lutcavage, M. E. and Lutz, P. L., Metabolic rate and food energy requirements of
the leatherback sea turtle, Derrnochelys coriacea, Copeia, 1986, 796, 1986.
1 2 Hormones in the Life
History of Sea Turtles
David W. Owens
CO NTENTS
12.1 IN T R O D U C T IO N
The presence of a chapter on hormones in a sea turtle book may surprise some
readers. As it turns out, the initiation of sea turtle farming on Grand Cayman Island
around 1969 was the initial stimulus for a long series of endocrine studies on sea
0-8493-8422-2/97/$0.00+$.50
© 1997 by CRC Press, Inc. 315
316 The Biology of Sea Turtles
turtles. Paul Licht of the University of California at Berkeley and Harold Papkoff
at the University of California at San Francisco were looking for a reptilian model
to be used in studies of the vertebrate pituitary gland. The project utilized hundreds
of fresh pituitaries from green sea turtles {Chelonia mydas) for purification of their
peptide hormones, which were then compared with many other species for structural
and biological properties.Subsequently, in an effort to improve captive breeding
potential, the Cayman turtle farm also supported many on-site endocrinological and
behavioral studies of green turtles^'^ and Kemp’s ridleys (Lepidochelys kempi)d'^
Because of this extensive record of endocrine study on sea turtles (see reviews by
Owens and Morris^ and Licht^^) they are generally the best known model for the
reptilian endocrine system. Indeed, because of the development and use of radioim
munoassays derived from sea turtle pituitary hormones,^^ we have also learned a
great deal about many other chelonian species.
While the focus of earlier reviews"^-^^ has been on the research done in the
laboratory and with captive populations, this summary will concentrate on the work
of the past decade which has been directed toward studies of natural populations as
well as species other than the green sea turtle. Sea turtles have been rather easy to
study during the brief period when the females emerge to nest or while the hatchlings
are developing and hatching. On the other hand, the remaining, completely marine
life stages constitute more than 99% of a sea turtle’s very long life. At these times
sea turtles are usually difficult to observe for any extended period. In addition, when
they are captured from the sea, it is often problematical to determine their sex, age,
and behavior/reproductive condition. For these reasons we have previously espoused
the use of endocrine evaluations, laparoscopy,^^ and more recently, ultrasound
imaging‘s to diagnose the specific life stage of the individual turtle. For example,
this type of diagnosis has proven very useful for choosing animals at specific times
in their reproductive cycle for use in satellite transmitter tracking studies.
to estrogens. Using leatherback eggs, these researchers found higher levels of aro
matase at 30.5°C than at 27°C and only observed estrogen production at the higher
temperature. Their model basically states that if the higher female-producing tem
peratures do not occur in an individual, there will be no aromatase, no estrogens,
and the embryo will remain a male.
Additional support for the aromatase-estrogen theory for female differentiation
in sea turtles is from the work of Crain et al."° and Gross et al.,^^ who measured
steroids in the chorioallantoic/amniotic fluid of hatchling loggerheads. They found
the ratio of estrogen to testosterone is higher in hatchlings raised at warmer tem
peratures. They also proposed the use of this ratio as a noninvasive sexing technique.
In the search for the primary genetic mechanism for TSD, heat shock proteins have
also been suggested for the transduction of environmental temperature.
12.3 JU V E N ILE S A N D S U B A D U L T S
In the transitions which occur from hatchling to adult life, sea turtles grow in size
at up to four orders of magnitude from their hatching weight and spend an estimated
10 to 50 years in the process, depending on the species and the quality of the foraging
habitat. Indeed, sea turtles change so much in size that they require different habitats
and have different niche characteristics, depending on their size. Sea turtle scientists
have come to describe these different situations as developmental habitats.
318 The Biology of Sea Turtles
FIGURE 12.1 Corticosterone in loggerhead hatchlings shows two pronounced peaks. The
first occurs at hatching and the second when the turtle first enters the water at four to five d
of age. It is at the low points that imprinting may be occurring. Different letters above bars
represent significant differences in means for a time using repeated measures ANOVA. (From
Schwantes, N., M.S. thesis, Texas A&M University, College Station, 1986. With permission.)
Although the supporting data are circumstantial at this time, we propose that dis
tinctive endocrine changes accompany and potentially regulate some of these devel
opmental habitat changes. Initially, as discussed above, B surges during the transi
tions from egg to hatchling as well as subsequently as the hatchling emerges from
the nest and migrates down to the water (Figure 12.1). During the pelagic phase we
know nothing about sea turtle homiones; however, post-pelagic immatures show
measurable testosterone with males having a seasonality and apparently temperature
dependence correlated to their testosterone levels (Figure 12.2). Is it possible that
this spring and summer surge in testosterone could be inducing turtles to move to
better or different foraging grounds? Indeed, seasonally speaking, when do sea turtles
make habitat changes? One certain time is in the cooling months when many tracking
studies have shown that turtles will leave for warmer areas.
According to Limpus,^^ sea turtles have a rather lengthy subadult period. In this
discussion we are equating subadult with the period of pubertal changes. It is during
this time that we first see the transition away from a temperature-related (controlled?)
androgen cycle to what we believe is a photoperiod-related (controlled?) cycle.
Figure 12.3 shows the dramatic early season peak in testosterone well prior to a
modest peak shown in immatures (Figure 12.2). We further propose that the initiation
of this photoperiod-related testosterone surge in subadults is also the time for the
Hormones in the Life History of Sea Turtles 319
I
FIGURE 12.2 Circulating testosterone levels in immature male Kemp’s ridleys at Sea
Arama, Galveston showing clear temperature influence at this age. (From Morris, Y., M.S.
thesis, Texas A&M University, College Station, 1982. With permission.)
transition to the final developmental foraging habitat where they will remain and
from which they will initiate their reproductive migrations. Sea turtles do have a
well-developed photosensitive pineal gland which produces the hormone melatonin,
which has been shown to regulate reproductive cycles in many vertebrate species.
We have previously reviewed this literature.
The sex of immature sea turtles is difficult to determine with external evaluations.
For several years we have been using the higher testosterone levels seen in immature
males as a sex-distinguishing technique for generating sex ratios. Because of TSD
in sea turtles, it has been important to generate sex ratios for use in population
modeling as well as to attempt to explain in evolutionary terms the biological
importance of TSD systems generally. Table 12.1 summarizes some of the published
320 The Biology of Sea Turtles
Time of year
FIGURE 12.3 Circulating testosterone from 80 adult loggerheads netted from Cape Canav
eral Channel between January and August. Note apparent separation into reproductively active
and inactive subsets. (From Wibbels, T., Owens, D. W., Morris, Y. A., and Amoss, M., in
Ecology o f East Elorida Sea Turtles, Witzell, Ed., U.S. Department of Commerce, NOAA
Tech. Rep., NMFS-53, Miami, FL, 1987. With permission.)
observations to date where testosterone has been used successfully to sex sea turtles.
It has been our practice when possible to use laparoscopic examination to indepen
dently verify the hormone technique. In most cases to date the laparoscopy has
proven the effectiveness of the testosterone-based system (Table 12.1). In two exper
iments, one by Schroeder and Owens^^ and a second by Coyne and Landry,
however, the testosterone technique produced ambiguous results. In the first case,
cold stunned greens, rescued from Mosquito Lagoon, FI, were transported to Orlando
where they were maintained for six weeks in tanks at 25 to 26°C. The testosterone
levels derived from blood samples taken after the six weeks of recovery did not
show the normal bimodal set of peaks, with females having a low peak at 0 to 20
pg/ml and males a higher peak above 30 pg/m\P Because the authors were able to
determine the sex of 66 other turtles which had died in the same cold-stunning event,
a good approximation of the expected sex ratio was available (1.77F:1.00M). Since
the hormone levels did not conform to this ratio, these authors felt the winter warming
of the surviving turtles may have caused an unusual increase in testosterone secretion
which smoothed out the expected bimodal curve, rendering the sexing technique
useless. Captive stress may also have been involved, since Coyne and Landry’s
unpublished work^"^ on Kemp’s ridleys indicates that the initial sample did predict
sex well, but that subsequent samples, taken over the next several hours, showed
increased variability in testosterone levels. Since captive stress is well known to
cause alterations in testosterone and since testosterone is temperature sensitive^“^ in
sea turtles, it is strongly recommended that all blood samples be taken as soon as
possible (within 15 min) after capture of the turtles. Since other blood-transported
Hormones in the Life History of Sea Turtles 321
TABLE 12.1
Sex Ratios of Immature Sea Turtle Populations Based on Testosterone Levels
Species #/Sex T pg/ml Ratio F:M laparoscopy Research site
factors may also be impacted by stress, this recommendation is relavent to all types
of physiological work on turtles.
As mentioned above, juvenile and subadult males begin to secrete more and more
testosterone as they age. Morris^^ observed that there is a positive correlation with
ambient temperature and testosterone in these young animals. Early testosterone
injection studies at the Cayman Island Turtle Farm showed that both the tail and
enclosed penis elongate predictably when this hormone or gonadotropin is
322 The Biology of Sea Turtles
12.4 E N D O C R IN E PATTERN S D U R IN G
R E P R O D U C T IO N
Ecological modelers of sea turtle populations require accurate numbers on sex ratios,
percent of animals reproductively active each year, and age to and duration of
reproductive life history. For several years we have been attempting to use hormone
levels as an indirect way to determine the reproductive and behavioral status of the
individual sea turtle. There are many reasons why this would be a useful
capability in these species. In the first place, female and possibly some male sea
turtles are not always reproductively active each year. While we have found that the
ridley species often reproduce in sequential years, they do also regularly skip years.
The larger sea turtle species rarely show repeat nesting in the next season, with the
exception of captive maintained turtles.Thus, when we see a turtle in the water it
is not usually clear what its reproductive state may be. Specifically, time of year or
the location where the turtle is captured does not always predict reproductive con
dition as it might in many species. Particularly in areas where both feeding and
reproductive populations coexist in the same habitat (Heron Island, Australia being
a good example), reproductively mixed populations can be found. On the other hand,
all of the turtles at a place like Ascension Island or Tortuguero, Costa Rica are
thought to be reproductively active since there are limited foraging grounds near
these nesting beaches.
Hormones in the Life History of Sea Turtles 323
FIGURE 12.4 (a, b, c) Photos of a 99-cm cci subadult loggerhead male: (a) shows the front
flipper claw is starting to curve; (b) the tail is clearly elongating, but will get much longer;
(c) the midline of the plastron is softening and has no keratin (a black line marks the soft
area), (d) An adult Kemp’s ridley is shown with fully dekeratinized plastron and a fully
developed tail.
324 The Biology of Sea Turtles
Another reason why the hormone indicators can prove useful in adults is that
there are no clear anatomical features which relate directly to reproductive condition.
As far as we know there are no seasonal or reproductive color changes, or modifi
cations in secondary sex characteristics. There is, however, one possible exception
which needs to be examined more closely. This is the softening of the plastron of
adult males, as discussed above."^^
An additional reason for using hormone indicators of reproductive status is the
confusion that commonly occurs between very large subadults and adults. As
Limpus^^ has noted, one can locate some very large individuals which are still not
reproductively active, and indeed they may be several years from adulthood. In some
cases it is even possible to misidentify a large subadult male as a female due to his
lack of secondary sexual characteristics. Limpus^^ was able to describe the occur
rence of this situation by employing laparoscopic surgery.
A final consideration is that laparoscopic surgery, followed by biopsies where
appropriate (in males), is certainly an effective way to determine reproductive con
dition of both males and females. However, this is an involved technique which
requires specialized training, considerable field equipment, and some risk to the
turtle. Eventually it is hoped that a blood sample, which is quickly and easily
obtained for hormone analysis, will suffice for a full determination of the individuaTs
reproductive condition.
12.4.1 Females
The endocrinology of reproductive adult females is better studied than other sea
turtle life stages because it was the emphasis of the early work at the Cayman turtle
Farm as well as subsequent field projects with olive ridleys, greens, and loggerheads.
Crawling, digging, and nesting females are also very obvious, accessible, and easily
bled for hormone analyses.
At Heron Island, Wibbels et al."^^ used the rodeo technique to collect from 4 to 13
reproductively inactive adult females per month between July and November. Several
of these turtles were known to have nested 1 or 2 years previously, they all had
atrophic or early growth ovaries upon laparoscopic examination, and their hormone
levels showed no significant seasonal changes. On the other hand, the ovaries of the
10 to 20% of the captured females which were reproductively active at this time
showed dramatic follicular growth with hundreds of approximately 1.5-cm-diameter
follicles and significant increases in circulating hormones.
reproductive subset did not cause them to depart immediately on their migration.
Thus, it appears that the general increase in circulating steroids we observed in the
spring is not the stimulus for migration. On the other hand, just prior to the departure
of the reproductively active loggerheads from the Heron Island feeding ground, we
did observe a dramatic and consistent drop in circulating estrogen as testosterone
went to its highest level up to that time in the year (Figures 12.5 and 12.6). At the
Cayman turtle farm during the mating season (they were obviously not able to
migrate) this same dip in estrogen and surge in testosterone was observed 8 years
earlier in green turtles.^ A particular challenge with green turtles is that estrogen
seems to circulate at lower levels than it does in other sea turtles.Regardless of
this potential species-specific difference, it is clear the circulating testosterone surges
while estrogen drops off at the time of migration in the reproductively active female.
o
02
W
H
cn
o
H
(n
FIGURE 12.5 Circulating hormone levels for a single turtle indicate a sudden increase in
estradiol followed by a shaip drop just as testosterone makes a pronounced surge. As the
testosterone peaked the turtle migrated away from the feeding ground. Also note the relatively
low estradiol compared to testosterone levels. (From Wibbels, T. et al., Owens, D. W., Limpus,
C., Reed, P., and Amoss, M., Gen. Comp. Endocrinol, 79, 154, 1990. With permission.)
At the Cayman turtle fami, greens and Kemp’s ridleys followed the chronology
of mating as depicted in Figure 12.6 with a discrete receptivity period about 28 d
prior to the first nest and 14 d prior to the first ovulation. This same pattern has been
observed for greens and loggerheads in Australia.^^ '^^ Female black turtles (C. agaS'
sizi) may not conform to this chronology based on observations by Alvarado and
Figueroa,"^^ Owens et al.,'^'^ and Rostal et al.“^^ Similarly, the chronological sequence
of Migration — Mating — Ovulation — Nesting may not be a constant for olive
ridleys either, since we have seen several individuals at Nancite, Costa Rica which
did not appear to conform to this pattern. For some time we have been testing the
hypothesis that testosterone levels in females receptive to mating will be at their
season high. While this idea seemed to work at the Cayman turtle farm, and was
326 The Biology of Sea Turtles
1 2 3 4
REPRODUCTIVE SEASON (MONTHS)
FIGURE 12.6 A general model for endocrine control and correlations with reproductive
behavior in male (top) and female (bottom) sea turtles. The dashed boxes indicate behavioral
components which are poorly understood. In this example the female ovulated and nested
three times. The hormone curves shown have been placed on a common scale for relative
comparisons of the timing of changes. The maximum typical level in picograms or nanograms
of circulating homione is indicated for each curve.
generally supported by the loggerhead data from Heron Island and the olive ridley
study at Nancite (Figure 12.6), more work needs to be done on this interesting
question. The primary difficulty has been in confirming true mating events as
opposed to mounted pairs which were not actually copulating. This later situation
appears to be much more common than we anticipated (personal observations). We
suggest that if the hypothesized testosterone peak for mating receptivity holds up,
it could prove useful in understanding much about sea turtle mating systems. For
example, the group of mating C. agassizi observed by Rostal et al."^^ in Michoacan
did not have significantly elevated testosterone compared to nesters. Were they truly
mating? It is possible that the females were not in their receptive period, but were
simply caught off guard by the very aggressive males in this species.
12.4.1.3 Nesting
The physiological and behavioral events of nesting in sea turtles are quite dynamic
and surprisingly fast considering the size and reptilian ancestry of the animal. In
Figure 12.6 we have summarized several studies^’^^'^^'^^^’'^^ to track many of the
endocrine aspects of the prenesting and nesting process. Licht and associates^' first
Hormones in the Life History of Sea Turtles 327
FIGURE 12.7 Circulating levels of neurophysin and arginine vasotocin during stages of
nesting in the olive ridley sea turtle. Letters above means indicate significant differences as
explained in Figure 12.1. (From Figler, R. A., Mackenzie, D. S., Owens, D. W., Licht, R, and
Amoss, M. S., Gen. and Comp. Endocrinol, 73, 223, 1989. With permission.)
predict nest number quite precisely. Rostal et al.^^ were even able to use testosterone
levels to accurately predict which clutch and the total number of clutches laid by a
given nesting female.
Hormones in the Life History of Sea Turtles 329
Caret fa caretta
FIGURE 12.8 Circulating levels of several hormones during nesting stages in the logger-
head sea turtle. Nesting stages are E, emerging; BP, body pit construction; N, nest chamber
construction; EO, early oviposition; LO, late oviposition; C, covering body pit; R, returning
to the ocean. *Data taken from Reference 55. (From Guillette L. J., Jr., Bjorndal, K. A.,
Bolten, A., Gross, T., Palmer, B., Witherington, B., and Matter, J., Gen. Comp. Endocrinol,
82, 121, 1991. With permission.)
12.4.2 M ales
The male sea turtles are less well studied than females because they do not come
ashore. However, since their energy investment in gamete production is also less
complicated than females, we suspect that they have a simpler hormone-control
system.
Wibbels et al.^^ were able to sample 80 adult male loggerheads at the Cape Canaveral
Ship Channel between January and August (Figure 12.3). This unique man-made
330 The Biology of Sea Turtles
From the work at Heron Island"^^ and our experiences at Cape Canaveral as discussed
above , t he impression we have is that loggerhead males begin their reproductive
migrations before the females. Supporting this idea are the testosterone data for both
of these populations which show a seasonal peak for males that is earlier than in
the females. We also noticed that some males left the Heron Island foraging area
well before the females departed on their reproductive migrations, although some
males did not seem to leave at all. Several years ago we administered testosterone
to immature greens (30 to 50 kg), which resulted in courtship and mating attempts
in animals which had not yet even initiated spermiation.'’ At this time, however, the
Hormones in the Life History of Sea Turtles 331
TABLE 12.2
Spermatogenic Stages Observed in Loggerhead Sea Turtles Captured on Heron
Island Reef
Month and number of turtles observed
stage Seminiferous tubules j F M
M A M J J A S O N D
Adapted from Wibbels, T., Owens, D. W., Limpus, C., Reed, R, and Amoss, M., G e n . C o m p . E n d o c r in o l,
79, 154, 1990. With permission.
12.5 P IT U IT A R Y H O R M O N E S T U D IE S
As mentioned above, the pituitary hormones of the green sea turtle have been under
study for nearly 25 years. This aspect of sea turtle endocrinology has been of greatest
interest to the comparative/evolutionary zoologist. For this reason we will only
highlight some of the recent advances in this area.
The structure and function of the pituitary protein hormones have been of special
interest to the comparative endocrine community due to the sea turtle’s (any turtles)
clear link to the stem reptiles. Although perhaps an overly simplistic comparison,
they are seen as living relatives of the oldest amniotic vertebrates. Among turtles in
general, sea turtles happened to be chosen because large numbers of their large
pituitaries were available for study. Licht and Papkoff^ ^ reviewed their physiology
and structure studies of the three distinct glycoprotein hormones FSH, LH, and TSH,
showing LH to be the smallest, followed by TSH then FSH.^^ This is of interest
332 The Biology of Sea Turtles
because, for a long time, we thought that FSH and LH might be a single molecule
with a somehow more “primitive” function. In testing different hormone preparations
in different vertebrate species, they concluded that “The relative importance of FSH
and LH may vary among turtles, and the sea turtle may be more like that of mammals
and birds’’.^^ Thus sea turtles may not even conform to a standard turtle model.
Sea turtle prolactin (PRL) and growth hormone (GH) have also been of particular
interest to the endocrinologist. As far as we can tell they were the first large proteins
ever completely sequenced from a r e p t i l e . P R L is slightly larger at 198 amino
acid residues,^^ while GH weighs in at 190.^^ The predicted somatotropic role for
GH has been strongly supported, along with a potential role in final gamete matu
ration.^ Evolutionarily speaking, the structure of the sea turtle GH molecule is
reassuring since it maps out closer to the birds than anything else (Figure 12.9).^^
PRL has proven particularly enigmatic in turtles. On one hand we know the
detailed structure of the molecule, but on the other hand we do not know what it
does in turtles.^ This is particularly surprising since PRL is well known to have roles
in migration, osmoregulation, and reproduction in vertebrates such as birds.
ó
FIGURE 12.9 Molecular phylogenetic tree of GHs on the basis of sequence comparisons.
The “Unit Evolutionary Period” figures for the tetrapod and teleost trunks are estimated to
be 9.6 and 6.3. The numbers of observed amino acid changes per 100 links are shown on the
tree. (From Yasuda, A., Yamaguchi, K., Papkoff, H., Yokoo, Y, and Kawauchi, H.,Gen. Comp.
EndocrinoL, 73, 242, 1989. With permission.)
Moon^"^ detected T3 only in the captive animals. He also noted that captive sea turtles
typically have higher levels of T4 (10 ng/ml in greens) than wild turtles (2 to 3 ng/ml
in both greens and loggerheads) and hypothesized that this may be due to the captive
conditions of constant temperature and high protein diet. We have noted over the
years that many captive sea turtles are overfed and obese.
In captive greens at the Cayman turtle farm, Licht et al.^^ showed no variation
in adult green turtle levels of T4 over three annual cycles. At a Galveston, TX
aquarium, however. Moon et al.^"^ found a significant annual cycle for female Kemp’s
ridleys, with a peak in the winter and spring correlating with follicular development.
Rostal^ later duplicated this result with female Kemp’s ridleys at the Cayman farm.
In the only attempt at this sort of annual thyroid study in wild turtles, Wibbels et
al.^^ thought he also detected an annual cycle in females at Heron Island. Adult
males and immatures in all situations except the one described below (hibernation?)
showed no annual cycle for T4 .
Winter dormancy (hibernation?) has been described in populations of black
turtles (C. agassizi) in the Gulf of California^^ and loggerheads (Caretta carettd) in
Cape Canaveral Channel, FL.^^ Since the thyroid axis is often involved in hibernation
and metabolic adaptations in other animals, we hypothesized an involvement of T4
in these sea turtles. Haynes and MacKenzie^^ found a temperature dependency of
thyroid hormone binding in plasma using both in vitro and in vivo techniques in
greens, loggerheads, and Kemp’s ridleys. Similarly, when Moon^^ subjected captive
immature Kemp’s ridleys and greens to gradual cooling in order to simulate hiber
nation conditions, the turtles showed greatly reduced circulating T3 and T4 levels
compared to controls. At the same time, the turtles being cooled showed reduced
feeding and activity levels and appeared to be exhibiting the dormancy conditions
as described for wild turtles. The thyroid axis clearly changed under these conditions;
however, no direct cause/effect relationship with hibernation was verified by these
protocols.
In 20-d-old hatchling loggerheads, Moiris^^ first showed that the hormone corticos
terone could be significantly elevated when the animals were under stress. She
exposed the turtles to 15 min of repeated dropping onto a sand substrate from a
height of only 3 cm. Hatchlings which were three d old showed a similar, but
nonsignificant response to the same stress, possibly because their background levels
of corticosterone were naturally three times higher than the older hatchlings. In a
study of captive juvenile loggerheads, Schwantes^^ found a distinct diurnal pattern
of corticosterone secretion, with highest levels occurring in the early morning. This
peak probably correlates with the highest activity period of the day for the species.
Juvenile loggerheads stressed by neF^ or trawl capture^^’^^ also had significantly
elevated corticosterone. Studying adult olive ridleys, Schwantes^^ found that mating
males which had been hand captured in the ocean and males awaiting slaughter in
a severely overcrowded holding tank showed significantly higher corticosterone than
females under the same conditions. It was suspected that this sex difference was
related to the reproductive condition of these turtles, with females somehow less
334 The Biology of Sea Turtles
12.7 C O N C L U S IO N A N D SU M M A RY
Sea turtles have proven very useful in hormone studies for two important reasons.
First, they are relatively large animals who can therefore give blood easily and
repeatedly without suffering physiologically.^^ At Heron Island, for example, we
hand captured (turtle rodeo) and bled (15 ml) some females up to nine times between
July and December. Recaptures of these individuals indicated that all of these females
made normal migrations, had typically high fertility in their clutches, and produced
normal numbers of clutches that season. A second reason for their value in endo
crinology has been discussed above in that their commercial use provided pituitaries
for biochemical purification of protein hormones.
In addition to being useful models of adaptation, a solid reason for choosing
sea turtles for these types of studies is that they deserve and need the attention. Since
they fall into the category which one of my colleagues calls the ‘‘Charismatic mega
vertebrates”, they are inherently interesting. The good news is that we can take
advantage of this popularity at the same time as we look for practical ways to solve
conservation problems which man has created.
Unfortunately, because of the amazing diversity of solutions to problems that
animals have arrived at through time, it is definitely not easy to speculate on how
Hormones ¡n the Life History of Sea Turtles 335
or with what particular molecule a given species may have taken advantage of (in
an evolutionary sense) to solve adaptation problems. Sea turtles, for example, can
be said to be secondarily marine, in that their suspected ancestors were terrestrial
and aquatic. As a consequence, they appear to have secondarily arrived at or evolved
solutions to problems encountered by such behaviors as living in salt water, initiating
long migrations, open-sea navigation, using prenuptial gametogenesis, and so on.
As a consequence of the above behaviors, many of these adaptations in sea turtles
may be unique to their marine lineage. This possibility further argues for continued
basic research on the physiology and behavior of these unique and often endangered
species.
1. Yearling and older immature male sea turtles of all species tested maintain
higher levels of circulating testosterone than females. This difference is
useful in detennining sex ratios for these species since secondary sexual
characteristics do not appear for several years and since sea turtles have
TSD.35
2. At hatching, the chorioallantoic/amniotic fluid contains steroids from the
embryo. The ratio of estrogen to testosterone is greater in the fluids from
hatching females than from males. These data have been used in a non-
invasive technique to generate a sex ratio from freshly hatched
clutches.^^’^^
3. Where direct observation is limited, estimating the number of clutches
produced by a female in a season has been difficult. The level of test
osterone predicts clutch number in some species; thus it may prove useful
in quantifying clutch numbers.
4. In satellite tracking studies, testosterone levels in the female and the male
have proven useful in predicting migratory, nesting, and mating behavior.
5. Corticosterone correlates to level of stress at several stages in the lives of
sea turtles. Using corticosterone in wild, injured, or captive sea turtles
may eventually prove useful in evaluating survival prospects and general
health.^^’^^^
6 . A sudden peak in progesterone predicts the time of ovulation in sea
turtles.^’^
7. AVT is important in oviducal contraction at the time of n e s tin g .^ In
clinical situations it is often possible to force oviposition by injections of
AVT or oxytocin.
8 . By using a composite of circulating estrogen, testosterone, and progest
1. Birds appear to be a very useful model for marine turtle adaptation. Levels
of corticosterone in hatchlings show peaks and dips at times appropriate
for imprinting style learning to occur.
2 . Steroids play a pivotal role in sex determination in embryos. It appears,
however, that an extrinsic factor may be the initial switch to one sex or
the o t h e r . M u c h more work needs to be done in this area.
3. Environmental temperature plays a role in modifying the circulating levels
of steroids in immature sea turtles. For example, testosterone levels are
significantly higher in warmer water.^^
4. Environmental temperature does not correlate with circulating levels of
steroids in adults. On the contrary and hypothetically, photoperiod may
provide the primary seasonal cues for reproduction in these prenuptial
species.
Capture stress can alter circulating testosterone levels which can in turn
be correlated with higher corticosterone levels, and, in the case of nesting
females, altered progesterone levels.
6 . Secondary sexual characteristics are induced by elevated levels of test
osterone.^ In the case of the softening of the plastron, this has not been
verified."^^
7. There are clearly two gonadotropins in sea turtles (FSH and LH). Circu
lating LH dynamics are consistent with a role in ovulation and progest
erone production. FSH also peaks at the time of ovulation, but has not
been well studied and no distinct role is evident.
8 . Sea turtles produce PRL in their pituitary. At this time we have no idea
what PRL does in sea turtles.^
Thyroid honnones (T3 , T4 ) remain fairly constant in immature sea turtles
under “normal” temperature fluctuations. In the lab, turtles exposed to
very cold conditions (down to IHC) demonstrated a significant drop in
T3 and T4 and appeared to approximate a state of hibernation.^’^^
10. The timing of migration correlates closely in both sexes with the peak
testosterone level during the reproductive season.
11. Even though testosterone is elevated during mating receptivity in the
female, it is still not clear if testosterone defines and controls the mating
receptivity in sea turtles.'^^
12. Estrogen circulates at unusually low levels in greens and at low levels in
all sea turtles. A full appreciation for the seasonal dynamics and gona
dotropin controls over estrogen is needed.
A CK N O W LED G M EN TS
A special thanks to my former and present students whose sweat and even blood
have gone into the gathering of these data. Dr. Janice Grumbles and Rhonda Patterson
also put a huge amount of work into many assays which are reviewed here. Drs.
Hormones in the Life History of Sea Turtles 337
Duncan MacKenzie and Roldan Valverde generously critiqued portions of the manu
script. The research from our lab has been supported by grants from the National
Science Foundation (BNS-8819940 and IBN-9124014) and the Texas A&M Uni
versity Sea Grant College Program, supported by the NOAA office of Sea Grant,
Department of Commerce, under grant number NA16RG0457-01.
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Hormones in the Life History of Sea Turtles 341
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'j Q Salt, Water, and pH
Balance in the Sea Turtle
Peter L. Lutz
CO N TEN TS
13.1 INTRODUCTION
Sea turtles live in a medium that is almost three times more concentrated than their
body fluids, some ions having even greater plasma/sea water gradients (i.e., 23-fold
for Mg^+, Table 13.1). Like most marine vertebrates, therefore, sea turtles must
continuously combat the problems of water loss and salt gain. How they maintain
internal osmotic and ionic homeostasis and how they regulate the related acid-base
balance is the subject of this chapter.
0-8493-8422-2/97/$0.00+$ 50
© 1997 by CRC Press, Inc. 343
344 The Biology of Sea Turtles
TABLE 13.1
Salt Concentrations (mm H ) of Sea Turtle Plasma and Seawater, and the
Ratios of Average Plasma Concentrations to Those of Seawater
Osmotic
Na K Ca Mg Br Cl pressure Ref.
For sea turtles the major potential routes for water loss are the respiratory tract,
the kidney, the salt gland, the gut (feces), and the integument. The major routes for
salt gain are the integument and gut (ingestion). In essence, these pathways are
combated through: (1) structural devices that act to mechanically reduce water and
salt flux and (2) physiological mechanisms that work to actively extrude gained ions,
obtain free water, and regulate the internal ionic millieu.
Although sea turtles appear to maintain tight control over the osmotic and ionic
composition of their plasma while they are at sea,^’^ it should be pointed out that
under special circumstances they can withstand substantial internal changes. Holmes
and McBean^ found that juvenile green sea turtles {Chelonia mydas) tolerated a fall
in plasma sodium from 157.8 mmol to 130.2 mmol 1~^ during 2 months in fresh
water, and Staub"^ reported a fall in plasma osmolality from 270 to 207 mOsm in
green sea turtles after about 14 weeks in fresh/brackish water (l.l ppt). A similar
robustness with regards to changes in internal salt/water balance is also suggested
from the old observations that captured green sea turtles can survive up to two
months held on ship deck, provided they were kept in the shade and occasionally
wetted with seawater:^ an ability that made them an invaluable source of freshmeat
for the early European mariners in the Caribbean.
While respiration can be a major source of water loss for terrestrial air-breathing
vertebrates,^ this route is probably minimal for sea turtles since the relative humidity
of the ocean surface air that they inspire will be about 100% and the expiratory gas
will have a similar water content.^ On those occasions when the turtle has an elevated
body temperature (see Chapter 11) some respiratory water loss will occur, but this
will be mitigated by the high efficiency of the sea turtle lung in extracting oxygen,^
since the greater the amount of oxygen uptake per breath, the less the ratio of
respiratory water loss/oxygen consumption.
Salt, Water, and pH Balance in the Sea Turtle 345
For an aquatic animal the rate of water loss across the integument will be a
function of its permeabilty and the transcutaneous osmotic gradient. The reptilian
epidermis differs from that of the mammal in having two types of keratinous protein,
a-keratin (hair-like) and (i-keratin (feather-like). In sea turtles the neck and leg have
a-type keratin while the shell scutes have p-type.^ Although the thickness of keratin
will be a factor in impeding cutaneous water loss by increasing the diffusion path
length, the primary barrier to water diffusion is provided by the lipid embedded in
the keratinous framework.^ In these respects the structure of the marine turtle skin
and shell indicates a high resistance to water diffusion.^’^^
Water loss from the passage of feces or urine has not been quantified, but the
estimates of very low rates for cloacal salt loss (see below) indicate that these routes
are likewise minor. By contrast, the salt gland provides a net gain of water (see
below).
FIGURE 13.1 Esophageal papillae in leatherback sea turtle with entrapped jellyfish.
underneath and have a thick cornified layer on the s p i n e . B u t they probably play
a role in reducing seawater intake. X-ray studies on green sea turtles using barium
solutions indicate that ingested food and seawater are initially prevented from passing
from the esophagus into the stomach by the closing of the sphincter. The contraction
of the esophagus then expels seawater out of the mouth or nares while the papillae
act to trap the food.^^ After this action the sphincter relaxes, allowing the passage
of the food bolus to the stomach. Significantly, hawksbills, loggerheads, and green
sea turtles have all been observed to forcefully eject seawater out of their nostrils
while feeding.
Although minimizing the osmotic burden from “incidental drinking” may be
important for adult sea turtles, there is evidence that hatchlings require some degree
of seawater intake. Bennett et al.*^ found that hatchling loggerhead turtles (Caretta
caretta), who can lose up to 12% of their body weight during emergence from the
nest, drink about 4 ml seawater 100 g'^ day"^ when placed in seawater and regain
their initial weight within about 10 to 15 d even without feeding. However, if not
allowed to drink they rapidly lose weight through dehydration. Marshall and
Cooper^® also found that hatchling Chelonia mydas in seawater gain weight before
they start to feed and calculate a similar drinking rate (2.4 to 8.5 ml seawater 100
g-i day~0- Drinking has also been recorded in juvenile green sea turtles. Using phenol
red as a marker. Holmes and McBean^ found that unfed juvenile green sea turtles
ingested 1.33 ml seawater 100 day^k However, it has been argued that sea turtles
normally avoid drinking seawater.
Salt, Water, and pH Balance in the Sea Turtle 347
With the apparent exception of the marine mammals, the kidneys of marine verte
brates are insufficient to handle their salt influx and all have extrarenal mechanisms
for excreting salt. As a group, reptiles are unique in the variety of organs used as
salt glands which include modified salivary glands (sea snakes), lingual glands
(crocodiles), and nasal glands (lizards).In sea turtles, salt excretion is performed
by highly modified lachrymal glands, whose function was first discovered and
investigated by Schmidt-Nielsen and Fänge.^"^
13.3.1.1 Size
The sea turtle salt gland is one of the more conspicious organs in the skull (Figure
13.2) whose importance perhaps can be gauged from the fact that in the leatherback
the salt gland mass is almost twice that of the brain^^ (alternatively, of course, this
may indicate the relative unimportance of the brain for these animals). Interestingly,
hatchlings have proportionately much larger salt glands than adults — i.e., for
hatchling green sea turtles (53 g) the salt gland is 0.3% body weight and for 100-
to 200-g leatherbacks the salt gland is about 0.4% of body w eight,w hereas for
large Caretta carreta (130 kg), the salt gland is only 0.046% body w eig h t and for
40-kg green sea turtles the salt gland is 0.045% body weight.^ In the smaller ridley’s
turtle (Lepidochelys oliváceo) weighing 9.1 kg, the salt gland was 0.067% body
weight.^ This large difference in relative salt gland weights between hatchlings and
adults may be related to the necessity for hatchlings to drink seawater when they
first enter the sea (see above). By comparison the relative salt gland weight for a
wide range of sizes of sea bird species is about 0.08% body weight.^^
13.3.1.2 Structure
The gross, histological, and ultrastructural morphology of the sea turtle salt gland
has been well studied (e.g., Schmidt-Nielsen and F ä n g e ,Ellis and Abel,^^ Abel and
E llis,an d Marshall and Saddlier).^^
Briefly, sea turtle salt glands are modified lachrymal glands, each gland consist
ing of about one hundred lobules separated by blood vessels and connective tissue.^"^
The lobules contain many blind-ending secretory tubules which drain into a central
canal within each lobule, and the central canals combine to form secondary ducts
and a sac-like main duct which opens into the posterior canthus.^^’^^ The central
canal and secretory tubules have a rich blood supply^^ and there is extensive vascu
larization of the duct.^^ The central canals are surrounded by broad sheaths of
connective tissue with a rich network of cholinesterase staining nerve fibers around
each tubule; a nerve network reactive to monoamine oxidase is also found in the
perilobular connective tissu e.T h e tubule is composed of three types of cell: small
basophilic cells in the tubule terminal segments, principle secretory cells separated
348 The Biology of Sea Turtles
FIGURE 13.2 MRI of head of leatherback sea turtle showing salt gland ducts converging
at the upper posterior region of the eye socket (acknowledgements W. Coles and A. Schulman).
by broad intercellular spaces, and small oval basal cells in the body of the tubule.
The principle secretory cells are characterized by having large quantities of mito
chondria and have convoluted lateral membranes that interlock with similar processes
in adjacent c e l l s . T h e surface area of the principle cells that is exposed to the
extracellular fluid is, in consequence, extensive.The intercellular channels of the
principle cells of sea turtles are filled with a mucopolysaccaride, a feature not seen
in bird salt glands,^”^and the main duct of the turtle salt gland is lined with mucocytes
which secrete a thick luminal layer of mucus lining the duct lumen.^^ Marshall and
Saddlier^^ suggest that the general organization of the sea turtle salt gland duct is
structurally more similar to that of the main duct of the elasmobranch rectal gland
than the ducts of the avian nasal gland, possibly indicating a functional similarity
between the elasmobranch rectal gland and the sea turtle salt gland.
When the salt gland is not active the loggerhead sea turtle produces a scanty secretion
that is isosmotic with plasma, i.e., 300 to 400 mosmol kg"^ (Figure 13.3). However,
when the gland is stimulated, tear concentrations rise rapidly to a plateau of about
1680 to 2000 mosmol kg“^ average 1900 mosmol kg~* (Figure 13.3), showing that
the sea turtle salt gland discharges a fluid with a concentration more than six times
that of the blood and twice that of seawater (Table 13.2). This all or nothing plateau
Salt, Water, and pH Balance in the Sea Turtle 349
o
£
FIGURE 13.3 Changes in flow rate and concentration of salt gland fluid in the green sea
turtle following salt gland activation (time zero). (From Nicholson, S. E. and Lutz, P. L .,/.
Exp. Biol., 144, 171-184, 1989. With permission.)
is maintained for the duration of the response period, after which it rapidly declines.
The concentrating power of the sea turtle salt gland is greater than most marine
birds achieve^^ and twice that of the shark rectal gland.^^ In practical ternis the result
is that the osmotic load incurred by a sea turtle ingesting 1 1 of seawater would be
unburdened by the secretion of about 500 ml tears, leaving a net gain of 500 ml free
water.
Chloride and sodium are the predominant ionic components of sea turtle tears,
but, unlike the salt gland fluid of the bird, which is almost pure NaCl,^^ green turtle
tears have substantial amounts of magnesium (36 to 54 mmol 1~0» calcium (20 to
36 mmol LO» potassium (12.5 to 30 mmol LO? bicarbonate (5.5 mmol ), and even
bromine (2.2 to 6.24 mmol LO (Table 13.2).^^'^^ Avian salt gland fluid has, by
contrast, less than 1 mmol magnesium and calcium combined.Turtle salt gland
fluid also contains appreciable amounts of urea (8.9 mmol LO at concentrations
similar to those found in blood. But interestingly, the urea levels remain constant as
osmotic pressure increases, suggesting that this osmolyte is in passive equilibrium
across the salt gland duct and is not being actively added to or removed from the
salt gland fluid.^^ Glucose concentrations are much less than blood (0.5 mmol
salt gland fluid, plasma 8.0 mmol 10. indicating that glucose is either not entering
the salt gland fluid or is actively removed from it. Protein is virtually absent (less
than 0.01 mg mkO-^^
Comparing the ionic composition of sea turtle salt gland fluid with that of plasma,
the greatest gradient is shown by Mg^+ (about x 18), greatly exceeding the sixfold
difference in osmotic pressure. To some degree, this may reflect the ionic challenge
presented by seawater imbibation, e.g., Mg^+ has the highest seawater/plasma gra
dient of all of the common ions (x 24, Table 13.1). Interestingly, the relative
concentrations of the major ions, Na^and CL, in sea turtle salt gland fluid are much
closer to those for seawater (NaVCL = 0.89, green sea turtle tears; Na+/CL = 0.86
350 The Biology of Sea Turtles
TABLE 13.2
Salt Gland Fluid and Urine Composition (mmol H ) of Various Sea Turtles
Osmotic
pressure
Na K Ca Mg Br Cl NH 3 Urea (mosm b^) Ref.
seawater) than plasma (NaVCh = 1.30, plasma green sea turtle).It would appear
that the ionic composition of the sea turtle salt gland fluid reflects more the ionic
burden from ingested seawater rather than the composition of the fluid from which
it is derived (ecf/plasma). This indicates that not only is the salt gland important in
water economy, but it also is concerned in internal ionic regulation.
Nicholson and Lutz^^ found that the composition of the salt gland fluid was
fairly constant throughout the secretory period, and stimulating the salt gland by
loading the turtles with hypertonic NaCl solution or hypertonic sucrose solution also
had little effect on the ionic composition of the tears.This suggests that once
activated, the sea turtle salt gland produces a fluid of fixed composition. Some lizards,
on the other hand, can vary the composition of their salt gland secretions; in
particular, considerable changes in the NaVK+ ratios can be produced by altering
the size and nature of the salt load.^^ By contrast. Holmes and McBean"* found that
the Na+/K^ ratio of the salt gland fluid for green sea turtles did not change over time.
This inflexibility in the composition of sea turtle salt gland fluid may reflect that the
marine life of sea turtles presents them with more uniform salt “insult” compared
to the terrestrial lizards.
also been reported in birds. For example, McFarland^^ observed that in salt-loaded
sea gulls the salt gland secretion rate rapidly peaked and then dropped to a lower
level where it leveled off and eventually ceased. On the other hand, leatherback sea
turtles (Dermochelys coriacea) appear to cry continuously,^^ and hatchling green
sea turtles maintain high secretion rates throughout the secretory period.^^ It is most
likely that flow patterns relate to the nature of the salt loads naturally encountered,
the hatchlings having particular problems in maintaining water balance (see above)
and the soft-skinned leatherbacks, which feed on jellyfish, having a higher salt influx
than other turtle species. In the loggerhead the duration of salt gland activity was
affected by the salt load.^^ With salt loads of 2.5 to 10 mmol ion kg body mass”^
the salt gland was active for 3 to 8 h; salt loads of 20 mmol ion kg body mass“^
produced responses lasting 15 to 18 h.
Several authors report similar cephalic/salt gland Na“^ efflux rates for the green
turtle (119 p.mol 100 g^^ h“^ Holmes and McBean;^ 118 pmol 100 g~^ h~^ Kooistra
and Evans;^ *137.8 jiimol 100 g"^ h \ Nicholson and Lutz).^^ These values are in the
same range as other reptiles with salt glands 1 to 255 pmol Na+ 100 g'^ h"k^'^
However, on a short-term basis, very much higher maximal rates (10,700 p.mol 100
g 1 h'l) were obtained by small (28-g) hatchling greens.^*^ This extraordinary high
secretion capacity in the hatchling may be related to its need to drink seawater when
it first enters the sea.*^
Interestingly, the left and right eyes of individual turtles often show marked
differences in tear flow and concentration. This has been reported for subadult green
turtles,hatchling green turtles,^^ and leatherback turtles.^^ Unilateral differences
in flow from the nasal salt glands have also been reported in the desert lizard
Uwmastyx acanthinurus,'^^ so the phenomenon is not limited to chelonians. No
comparisons of the salt gland secretions from the two eyes of any avian species have
been reported, presumably because avian nasal glands empty into the nostrils where
the tears mix across an orifice in the nasal septum.^^
Interestingly, the fluid in the lumina of the sea turtle secretory tubules is isosmotic
with b l o o d , a situation similar to that found in birds where the initial fluid at the
blind end of the salt gland tubules of birds is also isotonic with blood.'^^ Marshall
et al.^^ '^^have suggested that a standing osmotic gradient across the duct wall causes
the primary fluid to be concentrated by water absorption as it flows along the duct,
and that further ionic modifications in the fluid composition are brought about by
ion pumps. Osmotic extraction of water across a standing gradient would explain
the independence of concentration and flow rate seen in the sea turtle salt gland,
since once the luminal fluid came into equilibrium with the standing gradient no
further water would be taken up, and increasing the flow would merely displace the
position along the duct where equilibrium is achieved.^^
An interesting new hypothesis concerning the production of high osmotic con
centrations has recently been put forward by Schmidt-Nielsen.'^'^ The proposed mech
anism, which compasses the working of insect malphigian tubules to the mammalian
kidney, involves the production of hydrostatic pressures in the salt secreting tissues
through the action of peristaltic contractions in the muscle surrounding the organ.
The raised hydrostatic pressure acts to concentrate the luminal fluid by forcing water
across membrane pores that are selectively permeable to water. Further studies on
the highly effective sea turtle salt glands are clearly called for.
On the assumption that concentration of the expressed salt gland fluid is mainly
the result of an osmotic transfer of water across the duct wall, Nicholson and Lutz^^
calculate a maximum secretory rate 9.41 ml kg~* h~^ into the secretory tubules and
that 83% of the water from this initial isosmotic fluid is absorbed as the fluid passes
down the ducts. On a mass salt gland basis, this translates into a maximal initial
secretory rate of 0.32 ml g salt gland~^ mim^ comparable to the glomerular filtration
rate for humans (0.5 ml g kidney“^ min O-"^^
While little is known of the mechanisms that control sea turtle salt gland activity,
there appear to be some similarities with the bird salt gland. In birds the primary
control for activation is neuronal acting via the cholinergic nerves, with hormonal
influence secondary.In sea turtles, cholinergic nerves also appear to be involved
in salt gland activation.^'^ The intracellular activation of salt gland secretion in
Malachlemys (the only turtle species to date investigated) is also similar to that of
birds in that it is mediated by changes in cyclic AMP and involves an increase in
intracellular calcium.'^^ Although there is no information on intracellular mechanisms
in sea turtle salt glands, it would be surprising if they were very different. The recent
finding that a melanocyte stimulating hormone in birds is targeted to directly activate
their salt glands'^^ is certainly worth following up in turtles.
13.3.2 K idney
The primary function of the kidney is to eliminate waste products and regulate the
electrolyte composition of the blood. However, in reptiles the cloaca, colon, and
Salt, Water, and pH Balance in the Sea Turtle 353
urinary bladder are also important organs which modify the urine produced by the
kidney.
A detailed treatment of the morphology of the sea turtle kidney is given by
Solomon.'^^ essence, sea turtle kidneys (Chelonia mydas) are flattened lobed bodies
closely applied to the posterior wall of the pleuroperitoneal cavity. The nephron can
be divided into several distinct segments: a glomerulus, similar in structure to that
of the mammal, a proximal tubule, an intermediate segment, distal convoluted tubule,
and collecting tubules. This complex nephron would appear to be fully equipped to
perform the functions of filtration, absoiption, and secretion. Unlike snakes or
crocodiles, turtles have urinary bladders.^ We have no information on the operation
of the bladder in sea turtles, but in fresh water turtles the bladder is involved in the
active transport of both Na+ and CU, the secretion of H+, and the reabsorption of
HC0 3 ~.2^’48 jg probable that the sea turtle bladder possesses similar ion transport
mechanisms.
The composition of urine from several sea turtle species is shown in Table 13.2.
Na^ and CU are the major ionic constituents, but are present at much lower concen
trations than the blood (Table 13.1). Only urine K+ values are substantially higher
than the blood. The greatest variations are seen in the ammonia and urea measure
ments. BJorndal"^^ found little ammonia and a substantial amount of urea in the urine
of C. mydas. By contrast, Prange and Greenwald^^ found substantial ammonia, but
little urea, and KhaliP® only found urea in the urine of one out of four C. mydas
tested (8 . 8 mmol/1). It is probable that the urinary concentrations of these two
constituents vary widely according the nutritional and water balance status of indi
viduals. In the fresh water Trachemys scripta, for example, ammonia can account
for from 4 to 44% of total urinary nitrogen and urea from 45 to 95% of total urinary
nitrogen.^ ^
Under normal circumstances the osmotic concentration of urine is isosmotic to
that of the body fluids. For example, osmolal urine/plasma ratios of 0 .9 5 ^ 2 ^nd 1.0^^
have been reported for C. mydas and 0.99 for the leatherback.*^ However, the urine
may become more concentrated than plasma after prolonged dehydration (for C.
mydas 1 0 to 2 0 d out of water the average osmolal urine/plasma ratio = 1.15).29
Interestingly, the sum of the measured constituents is very much less than the osmotic
concentration (less than one third),^^ indicating that there are substantial amounts
of dissolved constituents yet to be discovered. The glomerular filtration rate (GRF)
of the C. mydas kidney is 14.3 ml kg' *h~*,^2 y^j-y much higher than the banded sea
snake (Laticauda colubrina, GFR = 0.5 ml kg *h *)^2 ^nd three times that for T.
scripta in fresh water (GFR = 4.73 ml kg * h~*).^2 Qu a daily basis the green sea
turtle kidney filters about 340 ml fluid per kilogram which, compared to 2 to 6 ml
kg *day *urine production (calculated from Prange and Greenwald),^^ indicates that
greater than 98% of the filtrate is reabsorbed prior to voiding.
13.4 A C ID -B A S E B A LA N C E
In biological solutions [HH and [HCO3“] can be considered as dependent variables
whose concentrations are determined by the independent variables: the difference
354 The Biology of Sea Turtles
in charge between the sum of the strong ion electrolytes, the PCO2 , and the total
weak acid present.'’^ This means that the ionic composition and acid-base balance
of the blood are functionaly linked, and according to Stewart,changes in [H+] are
only brought about by alterations in one or more of these independent variables.
More importantly, solutions separated by biological membranes can interact in acid-
base terms only by processes that affect the values of the independent variables. The
pH of blood plasma is essentially determined by a C0 2 ~HC0 3 ~ buffer system that
can be described by the Henderson-Hasselbach equation:
where aC 0 9 is the solubilty coefficient relating [CO2 ] and PCO2 , and pKa is the
apparent pK of this reaction.
Since changes in pH have such wide metabolic effects, disturbances in acid-
base balance are generally resisted.
Temperature (®C)
FIGURE 13.4 The effect of temperature on blood pH in loggerhead sea turtles (a^^ and
on the air convection requirement in the green turtle (b)7*
13.4.3 Regulation of p H
In many reptiles, and particularly turtles, the temperature-related blood pH changes
are achieved through altering the efficiency of lung gas exchange, whereby the
increase in oxygen consumption (VO2 ) caused by a rise in body temperature is not
matched by an equivalent increase in lung ventilation (VE).^"^ This results in an
increase in blood PCO2 with body temperature and a fall in the / VO2 ratio
(called the air convection requirement, ACR). If the blood CO2 content and therefore
blood [HCO3 ], remain constant, blood pH is controlled by the change in PC0 2 .^^
Indeed, increasing body temperature results in an increase in blood PCO2 and a
decrease in ACR (Figure 13.4b) in both the green^^ and loggerhead.^^ But the
situation with respect to temperature-related changes in [HCO3 ] is somewhat con
fused by the fact that different authors have used different functions in calculating
the value of the apparent pK for the Henderson-Hasselbach equation (see above).
One of the most widely used set of values^^ only accounts for the effect of temper
ature on apparent pK and makes no correction for the effect of pH. The more accurate
Salt, Water, and pH Balance in the Sea Turtle 357
functions given by Severinghaus^^ which take into account both pH and temperature,
have been derived for the mammalian situation. Heisler^^ has developed complex
equations for deriving pKa values in ectotherms that have been successfully applied
to blood plasma of the Kemp’s ridley sea turtle.^^ Applying the Severinghaus^^
functions to the data for the green turtle of Kraus and Jackson^’ shows a decline in
bicarbonate with temperature, and a similar result has been calculated for the log-
gerhead.^2 indicates that in sea turtles the temperature-related adjustments of
blood pH are not simply a matter of respiratory adjustments of blood PCO2 , but are
also managed at the tissue levels. More direct evidence of the involvement of
nonrespiratory mechanisms in temperature-related pH regulation is seen in the log
gerhead, where the fall in blood pH produced by increasing temperature is associated
with an increase in plasma potassium.^^ An identical association between plasma K+
and body temperature has been found in a field study of a large number of “wild”
loggerheads.^ A similar relationship between blood pH and K+ concentration is found
in mammals and is thought to be the result of intracellular buffering of blood pH
whereby extracellular H+ ions are taken up by the cell in exchange for K+ ions."^^
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360 The Biology of Sea Turtles
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Salt, Water, and pH Balance in the Sea Turtle 361
CO N TEN TS
0-8493-8422-2/97/$0.00+$ 50
© 1997 by CRC Press, Inc. 363
364 The Biology of Sea Turtles
14.1 IN T R O D U C T IO N
Sea turtles are affected by a variety of health problems. Some of these problems are
naturally occurring processes, such as parasitism, and are observed in both wild and
captive animals. Other conditions, such as certain nutritional deficiencies, are essen
tially a result of prolonged captivity. The term captive animal will be used throughout
this chapter to indicate animals held in confinement, including those in oceanariums,
commercial farms, research institutions, and head-start programs.
The environment sea turtles occupy predisposes them to some of the disease
processes with which they must contend. For example, how a turtle deals with
exposure to an infectious organism and its response to physical trauma is greatly
affected by environmental stress. While little is known about the sea turtle immune
system, it can be assumed that, like other animals, they suffer a reduction in ability
to fight disease when stressed. In stressed sea turtles, the adrenal glands release
corticosterone^’2 which may reduce the humoral and/or cell-mediated defense mech
anisms of the turtle, thus inhibiting the ability of the immune system to respond to
infectious agents. Stresses can be environmental (salinity, pollution, temperature,
etc.), nutritional, or physical (trauma). Low ambient temperature has been shown to
reduce immunoglobulin production^ and the competence of the immune response in
reptiles.
This chapter describes some of the health and disease problems commonly
observed in both wild and captive sea turtles. Only those problems with high
morbidity and mortality rates or those syndromes which are commonly noted are
included. The health problems discussed here are grouped according to etiology. In
cases where the cause of a disease process is unproven or there are multiple factors
involved in the pathogenesis, the disease is described in the section concerning the
primary or most probable agent.
Health Problems and Diseases of Sea Turtles 365
14,2 N U T R IT IO N A L PR O BLEM S A N D R EQ U IR EM EN TS
Captive sea turtles have been fed a variety of diets and held under a variety of
conditions. They have been successfully raised, maintained, and propagated on
commercial pelleted turtle food, modified pelleted trout ration, and balanced gelatin
diets. Most prepared diets range from 25 to 35% protein, but diets up to 45% protein
have been used."^ Kemp’s ridleys^ (Lepidochelys kempii) in government-sponsored
head-start programs have been maintained by feeding them a commercial diet at the
rate of 5% body weight daily (BWD) to hatchlings and 1.5% BWD to yearlings. At
the Cayman Turtle Farm^ Grand Cayman, B.W.I., yearling green turtles (Chelonia
mydas) have been successfully fed a similar diet at the rate of 1.2% BWD, and 44-
month-old animals were fed 0.8% BWD.
14.2.1 M alnutrition
Wild sea turtles that are able to successfully fulfill their nutritional needs do not
appear to exhibit signs of nutrient deficiencies. Free-ranging animals found in a
weak and emaciated condition generally have a physical or medical problem that
inhibits their ability to procure food. Such problems can include damage to flippers,
mouth parts, or the eyes. Sea turtles suffering from a variety of chronic diseases
and/or carrying a heavy parasite burden may cease to feed for long periods of time
and waste away. Cachectic myopathy^ is the general wasting exhibited by nonfeeding
and/or heavily parasitized animals. These individuals are characterized by concave
plastrons, sunken eyes, muscular atrophy, and reduced weight. Analysis of blood
samples will often show anemia, hypoproteinemia, and hypoglycemia. Necropsy
reveals reduced muscle mass and little fat. In extreme cases, serous atrophy of fat
can be observed.
Cachectic myopathy can be observed in captive turtles as well as wild ones. Any
disease process causing cessation of feeding will eventually produce a malnourished
sea turtle. For example, hatchlings fed peneid shrimp^ and larger turtles fed inap
propriate vegetation^ have been noted to develop gastrointestinal (GI) obstructions.
Obstructed turtles will stop feeding, waste away, and die unless the blockage is
relieved.
Juvenile captive turtles regularly fed diets consisting solely of squid and fish have
developed life-threatening anemias.^ Kemp’s ridleys and loggerheads have been
presented for care because they were found listless, floating, and hyperventilating
due to poor oxygen-carrying capacity. On examination, packed cell volumes (PCVs)
varyied from 4 to 10%. This contrasts with normal PCVs for juvenile turtles in the
Chesapeake Bay, which range from 24 to 34%. All of the anemic turtles examined
were emaciated and showed signs of malnutrition. The animals responded well to
dietary adjustment and iron supplementation, after which they exhibited sequential
rises in their PCVs, weight gains, and increased activity.
turtles in close proximity to healthy turtles can “seed” the water with bacteria,
therefore increasing the likelihood of spread of disease. Any infectious agent, be it
bacterial, viral, fungal, or parasitic, can be easily transmitted in a closed system.
A variety of bacteria have been cultured from diseased sea turtles. The most
commonly cultured pathogens are Aeromonas hydrophila, Vibrio alginolyticus,
Escherichia coli, Citrobacter sp., Enterobacter sp., Proteus sp.. Pseudomonas ^p..
Salmonella sp., Mycobacterium sp., Edwardsiella sp., Arizona sp., and Elavobacte-
rium sp. Quite often, several species have been cultured from a single lesion. In
such cases it has not been clear whether only one type of bacteria was responsible
for the disease process or if the various bacteria functioned synergistically. Some of
the organisms cultured could have been contaminants and not related to the infection.
To further confuse the issue, some cultured lesions yielded fungal organisms in
addition to the bacteria.
There are a few reports of mycobacterial infections occurring in captive turtles.^ '^’^^
In an outbreak of tuberculosis, acid-fast rods were found in histological preparations
of the lungs, livers, spleens, and kidneys from 6 of 120 hatchling green turtles
collected from the wild. Signs of infection appeared in the six animals over a two-
month period, and all of the infected animals died. M. avium was cultured from all
of these turtles, and the investigators considered the possibility of airborne trans
mission. In a separate case, M. marinum was cultured from the lungs of an infected
368 The Biology of Sea Turtles
Bacterial infections of the dermis are both common and highly visible in captive
sea turtles, and a variety of bacterial dermatitis syndromes are described in the
literature.^’^’^’^^'^^ Names such as focal errosive dermatitis (FED), septicemia ulcer
ative cutaneous disease (SCUD), and papillary dermatitis (PD) not only identify a
type of bacterial infection, but describe the predominant lesions produced by such
an infection. The most frequently cultured organisms are Aeromonas sp., V. algi-
nolyticus, Pseudamonas sp., Proteus sp., and Citrobacter sp. The same genus or
suite of microorganisms may be cultured from more than one of these diseases; it
is not understood why a single bacterial species, such as A. hydrophila, may cause
a variety of different disease syndromes.
Commonly described lesions involve ulceration and discoloration of the dermis
(FED, SCUD). The ulceration may be superficial or deep. Dermal infections with
deeply ulcerative lesions (SCUD) may provide a route for bacteria to enter the
bloodstream and lead to lethal septicemias. Other bacterial syndromes, such as PD,
are more superficial and proliferative in nature, and are characterized by the devel-
opement of papilla-like projections.
Response of dermal infeetions to antibiotic treatment varies greatly. The best
results have been obtained with a combination of topical and systemic antibiotics.
Efficacy of antibiotic treatment is greatly enhanced by an improvement in water
quality. In addition, the spread of disease is reduced by isolation of sick individuals
and frequent water changes.
the fluke eggs played an active part in the development of a bacterial GME or were
a serendipitous finding.
Infections due to Clamydia psittaci are common in mammals and birds, but are rare
in reptiles. Only one occurrence of chlamydiosis in captive sea turtles has been
documented.^^ An epizootic disease outbreak at the Cayman Turtle Farm resulted in
the death of several hundred juvenile green turtles. Sick turtles were lethargic, weak,
and often found floating on the surface.
Lesions in necropsied animals were primarily confined to the heart and liver.
Several 1- to 10-mm gray patchy lesions were noted on the hearts. The livers were
enlarged and friable. Microscopic examination of the necropsy specimens revealed
necrosis of the myocardial and hepatic tissues. Chlamydial antigens were detected
in tissue samples from dead turtles, visualized with electron microscopy and cultured
in vitro.
When fungal organisms colonize the dermal tissues of sea turtles, the infection can
manifest itself in varying ways. Aspergillus sp. have been found in lesions on the
flippers of hatchling loggerheads.^ These animals had focal, black, dry, gangrenous
areas on the leading edges and tips of the front and rear flippers. The areas underwent
auto-amputation and resolved when treated with topical iodine. Leong et al.^
described a variety of necrotic lesions on the head, neck, and shell of head-started
Kemp’s ridleys. The lesions appeared to be due to a number of different fungal
pathogens, and varied from mild to severe. Water quality appeared to be an important
factor in the incidence of disease in both the Aspergillus outbreak and the fungal
infections in captive ridleys. In both cases, improvement in water quality, brought
about by changing from static to free-flowing water systems, greatly reduced the
incidence of infection. The improvement in water quality also increased the efficacy
of treatment provided for infected turtles.
370 The Biology of Sea Turtles
Systemic mycotic infections of wild and captive turtles occur primarily in the lungs.
Yet while essentially a pulmonary disease, mycotic granulomas also can be found
in the liver and throughout the coelomic cavity. Infected animals are listless and
often float or swim with one side lower than the other. Leong et al.^ described various
systemic fungal disease processes in head-started Kemp’s ridleys, including pulmo
nary mycoses, mycotic osteomyelitis, and yolk sac mycoses. The mortality rate for
affected turtles was very high.
In a group of juvenile captive green turtles with systemic mycotic infections,
Jacobson et al.^^ found a number of animals had a consolidated left lung and an
emphysematous right lung, explaining why these sick animals floated in a nonhor
izontal position. On necropsy the lungs were found to contain multiple firm, round,
white nodules of varying diameters. Histological examination of the lesions revealed
caseous necrotic material and branching septate hyphae, surrounded by a granulo
matous inflammatory reaction.
Paecilomyces sp., Sporotrichium sp., Scolecobasidium sp. and Cladosporium
sp. organisms have been isolated from infected t u r t l e s . T h e s e fungi have been
incriminated as the agents responsible for a variety of systemic mycotic infections.
Reduced body temperature and subsequent impairment of the immune system
of a turtle may predispose the animal to a mycotic infection. The cases of fungal
pneumonia reported in the literature often involve animals exposed to cold temper
atures. Lewbart and Medway^^ reported a turtle with mycotic lung disease found
floating off the coast of Delaware in October (no water temperature available). In
the outbreak of mycotic pneumonia described by Jacobson et al.,^^ mortality peaked
during the month with the lowest water temperatures.
isolated from the respiratory tract lesions, a herpesvirus was thought to play a major
role in the disease process.
14.6 FIBROPAPILLOMATOSIS
Cutaneous fibropapillomatosis is a major epizootic disease affecting a variety of
species of sea turtles. The predominant lesions associated with this disease are
fibromas, cutaneous papillomas, and fibropapillomas. Because it has been reported
primarily in green turtles, this disease syndrome has been designated green turtle
fibropapillomatosis (GTFP). Even so, GTFP has also been documented in loggerhead
turtles and there are reports of it occurring in olive ridley turtles (L. olivácea),
hawksbills (Eretmochelys imbracata), and flatback turtles (Natator depressus)?^
14.6.1 H istory
Lesions consistent with GTFP were first described in green turtles from Florida
waters in 1938,^^ and in 1958 GTFP lesions were reported in Hawaiian green turtles.
Since then, GTFP disease has been identified around the world, in captive turtles as
well as wild animals.
372 The Biology of Sea Turtles
14.6.2 Epidemiology
The incidence of turtles with GTFP has increased rapidly since the 1980s, but varies
between years and locations. In some populations no animals are affected, while
elsewhere up to 92% of individuals may exhibit GTFP lesions. Turtles frequenting
near-shore waters, areas adjacent to large human populations, and areas with low
water turnover, such as lagoons, have a higher incidence of GTFP than individuals
in deeper, more remote waters.
The lesions associated with GTFP seem to increase in size more rapidly in
summer,^^ but current statistics show that more diseased turtles strand in winter than
in summer. As the lesions grow and/or increase in number, they may cause the turtle
to become debilitated. External growths may impede the ability of the animal to
feed or navigate; internal growths can lead to pneumonia, liver disease, intestinal
obstruction, or kidney disease. While the disease slowly progresses in most turtles,
there are reports of spontaneous lesion regression occurring in some affected animals.
Body size and age class are associated with GTFP infection. Juvenile turtles
appear to be most affected: individuals in the 40- to 90-cm carapace length and 10-
to 30-kg weight class^° have the highest incidence of disease and the most extensive
lesions. Conversely, lesions in nesting adults are rare.
14.6.3 Pathology
As previously mentioned, the three primary lesions associated with GTFP are fibro
mas, cutaneous papillomas, and fibropapillomas. The number of lesions may vary
from single to many. They may be smooth or ulcerated, sessile or pedunculated
(Figure 14.1), small (0.1 cm) or large (30 cm), and cutaneous or systemic. Lesions
are commonly found in the conjunctiva, chin, neck (Figure 14.2), flippers, base of
the tail, and axillary and inguinal areas. The lesions are pigmented similarly to the
tissues from which they arise.
When present, visceral lesions occur in the lungs, liver, GI tract, and kidneys of
GTFP-affected turtles. This distribution of visceral lesions suggests a hematogenous
spread of the disease. Visceral growths are usually firm white nodules embedded in
the parenchyma of the affected organ. While the majority of lesions are well demar
cated, some growths may be characterized by an infiltrative margin.
14.6.4 H istology
Typically, the microscopic appearance of lesions associated with GTFP is one of
papillary epidermal hyperplasia and/or dermal hyperplasia.^The histological find
ings vary with the three gross lesion types. Papillomas are made up of hyperplastic
epidermis, fibromas consist of hyperplastic dermal tissues, and fibropapillomas are
characterized by a proliferation of both epidermal and dermal tissues. According to
Jacobson et al.,^^ the earliest detectable lesions are a ballooning degeneration of the
stratum basale and epidermal hyperplasia. Both the dermal cells and fibroblasts
present in these tissues are well differentiated and benign in appearance. The non-
malignant status of GTFP tumors is further supported by flow cytometric DNA
Health Problems and Diseases of Sea Turtles 373
data.^"^ These data indicate cells from GTFP lesions have a normal cell division cycle
and are benign in nature.
The presence of trematode eggs, perivascular lymphocytic cuffing, and multi-
nucleate giant cells are also common histologic features of GTFP. These features
are often present in other conditions, and the significance of these findings in GTFD
is open to speculation.
14.6.5 Etiology
14.6.6 D etection
Early detection of GTFP through the use of serological tests may help in defining
and monitoring the level of disease in sea turtles. Currently, monoclonal antibody
tests are being developed that may lead to a method for identifying turtles with GTFP
prior to development of visible lesions.
14.6.7 T reatment
There is no effective treatment for turtles suffering from GTFP. Once the étiologie
agent is isolated and purified, specific and effective treatments as well as preventive
measures may be developed. Currently, treatment is aimed at reducing the tumor
burden which affects the ability of the turtle to function normally. Tumors may be
surgically extirpated from the eyes and mouth to allow the animal to see and feed.
Also, fibropapillomas have been removed using cryosurgical techniques instead of
excisional surgery. Cryosurgery may stimulate the immune system of the turtle and
cause regression of tumors besides those frozen.
Health Problems and Diseases of Sea Turtles 375
14.7.1 Ectoparasites
14.7.1.1 Leeches
Leeches can occur in small or large numbers. They are most often located on the
soft tissue surfaces of the axilla and inguinal areas, but can attach around the eyes,
mouth, and cloaca. Aggregations of yellow egg cases may be found on the carapace,
plastron, and flippers.
Two significant species of leeches are known to parasitize sea turtles. Ozobran-
chus branchiatus is found only on green turtles inhabiting tropical waters. O. margoi
occurs on most species of sea turtles, is found on turtles inhabiting cooler waters,
and has a more worldwide distribution than O. branchiatus.
Leeches use their oral sucker to obtain a blood meal from the host. Animals
carrying large numbers of leeches become anemic and have extensive areas of
macerated dermal tissue. In addition to their debilitating effects, leeches can also
act as vectors for various disease-producing organisms. There may be a relationship
between turtles with large leech infestations and the occurrence of the GTFP.
Since leeches can complete their life cyle on the host turtle, captive animals can
rapidly develop severe infestations. Currently, the most effective way to remove
leeches from an infested turtle is a 90-min fresh-water bath.^^
14.7.1.2 Barnacles
Several varieties of barnacle are found on sea turtles,and large numbers of these
epizoans can contribute to the overall stress on a sea turtle. Some varieties only
negatively affect the animal by increasing surface drag, while other varieties can
damage the underlying shell and skin and allow bacterial or fungal pathogens to enter.
14.7.2 Endoparasites
A variety of endoparasites can occur in sea turtles. While the adult organisms may
have a deleterious effect on host animals, the majority of damage is done by immature
stages.
14.7.2.1 Protozoans
Pathological conditions due to amebic and coccidian parasites has been described
in sea turtles. Entameba invadens, which is ubiquitous in freshwater turtles and
376 The Biology of Sea Turtles
snakes, has been implicated in the death of both green and loggerhead turtles.^^ E,
invadens cannot live in salt water and has only been reported in captive turtles. The
amebae cause an enterohepatitis after being introduced to a turtle, most likely in
contaminated food. Animals with amebic enterohepatitis have high white blood cell
counts, high liver enzyme levels (SGOT/AST), and amebae in stool samples. If
detected early, the infection may be treated with metronidazole.
Outbreaks of coccidiosis among wild"^° and captive turtles have been ascribed
to Caryospora cheloniae. In 1991, an epizootic infection occurred among large
subadult green turtles in Moreton Bay, Australia. On necropsy, the turtles exhibited
severe ulcerative enteritis. Microscopic examination of intestinal scrapings revealed
large numbers of enlongated C. cheloniae oocysts. Both the intestinal lesions and
oocysts were similar to those occurring in a group of sick captive green turtles
described by Leibovitz et al."^^ Treatment with antibiotics and sulfonamides was of
no benefit to either wild or captive animals. Eimeria caretta has been described in
two loggerhead turtles.T here was no sign of intestinal disease in these animals,
but oocysts were found in routine stool samples.
14.7.2.2 Helminths
Sea turtles play host to a variety of helminths. While many genera of tremátodos
and nematodes are recorded in the literature, no cestodes are reported.
Sea turtles are hosts to a large variety of tremátodos. With only one exception,
these tremátodos are digenean. Sea turtles acquire such flukes by ingesting unknown
cercariae-rich intermediate hosts. The majority of adult flukes reside in the GI tract
and cause minor irritation and damage. Heavy infestations can produce clinical
disease and severely debilitate the host.
Spirorchid tremátodos are the most damaging parasites with which sea turtles
contend. Adult spirorchid flukes reside in the vascular system, whereby their eggs
are disseminated throughout the body. Spirorchidiasis is a chronic debilitating dis
ease of older juvenile loggerheads, and affects up to 30% of the Atlantic loggerhead
population."^^ Spirorchid flukes also occur in green turtles and hawksbill turtles, and
are found worldwide in both wild and captive individuals."^'^'^^
Adult flukes reside in the heart and major blood vessels, causing an epithelial
thickening of the vessel walls and vasculitis. The eggs become disseminated through
out the vascular system and result in a vasculitis and granulomatous reaction wher
ever they lodge. Common sites for lesions to occur are the brain, lungs, liver, kidney,
and intestines. Intestinal lesions are raised, linear, and discolored. These greenish-
black areas occur primarily in the hindgut, and when incised release a yellow-brown
gritty material made up of masses of eggs. Microscopically the lesions contain
multinucleated giant cells and exhibit perivascular lymphocytic cuffing.
The four most prominent genera of spirorchids inhabiting sea turtles are Hap-
lotrema, Learedius, Carettacola, and Neospirorchis. Their eggs are elongated and
often have hooked terminal processes. The negatively bouyant eggs may be found
in feces of infected turtles. These blood flukes may serve as disease vectors, and
their role in such diseases as GTFP is being investigated. To aid such investigations.
Health Problems and Diseases of Sea Turtles 377
as well as to study the epidemiology of blood fluke infections, ELISA tests have
been developed"^^ to detect antibodies to spirorchid flukes.
There are several nematodes known to inhabit the intestinal tracts of sea turtles.
The majority of these wonns are ascarid-like and reside in the pyloroduodenal area
of the GI tract. Nematodes such as Sulcascaris sulcata^^ are cosmopolitan in distri
bution and can be found in several species of sea turtle. Nematodes cause a mild
hemorrhagic enteritis and, unless they occur in large numbers, are of little clinical
significance. The negatively bouyant eggs of S. sulcata and other nematodes are
passed in the feces of the turtle. The eggs may then be ingested by molluscs which
serve as intermediate hosts.
14.8 E N V IR O N M E N T A L H E A LT H PR O B LEM S
A variety of health problems occur in sea turtles which may be directly or indirectly
brought about by local environmental factors. Some of these detrimental factors
occur naturally, such as rapid water temperature changes. Other factors involve
human activities, such as littering the oceans with anthropogenic materials.
Hypothermic or cold stunning occurs when a turtle is exposed to cold water for a
period of time. As the core body temperature of the turtle drops, it becomes unable
to function normally. When water temperatures drop to 10°C, turtles bob to the
surface and float ineffectually. If temperatures drop to 5 to 6 °C death may occur.
Cold-stunning events have occurred from the Gulf of Mexico to New England, as
well as in Western Europe. In-depth reviews of such cold-stunning events have been
reported by a number of authors.'^^ '^^
Inshore populations of turtles are most susceptible to cold stunning because the
water temperature can change rapidly in shallow waters. Turtles inhabiting shallow
lagoon systems are particularly at risk when the weather cools rapidly. Not only will
a lagoon system sustain a rapid temperature drop, but turtles may be unable to easily
escape the lagoon and flee the cooling waters. Smaller animals cold stun sooner
than larger ones. Recorded cold-stun events have included loggerheads, greens, and
Kemp’s ridleys.
The main goal in treating a hypothermic turtle is to gradually raise the core body
temperature of the animal by immersion in warm water. A variety of medications
378 The Biology of Sea Turtles
have been suggested to enhance body functions and aggressively treat severely
stunned animals.
Salt glands normally provide a way for sea turtles to remove excess sodium,
potassium, chlorine, calcium, magnesium, and phosphorous from their systems.
Cold-stunned animals often have elevated values for these ions.^"^^’^^ Turtles that
have survived cold stunning often have decreased salt gland function for a short
period of time. Such animals may be maintained in fresh water until salt gland
function returns and plasma electrolytes approach normal levels.
Sea turtles often become entangled in a variety of fishing gear, lines, cables, or
refuse. In the broadest sense, capture in a shrimp or flounder trawl can be considered
a form of entanglement. Either of two basic types of injuries may occur when a sea
turtle becomes trapped in a net or other man-made object.
First, a local injury may occur where the turtle is caught in the fishing gear. An
example would be a linear necrotic band around a flipper that is wrapped in a crabpot
line. The injury may be superficial or deep, and may even require the amputation
of the flipper.
The second type of injury occurs when a turtle is caught in a gill or trawl net
and is kept submerged for a prolonged period of time. It may or may not drown, as
some are able to hold their breath for lengthy periods. When rescued, some trapped
turtles may be in an anoxic state and comatose.^^ The casual observer may not notice
a very occasional heartbeat or even a shallow breath. An areflexic turtle removed
from a trawl net should be carefully checked for any signs of life. Even if no life
signs are noted, the animal should be placed in the shade and monitored for several
hours. With proper supportive care, some of these animals may be revived. The brain
has biochemical adaptations that allow sea turtles to withstand lengthy periods of
anoxia^^ during prolonged dives or hibernation. This normal response to anoxia may
therefore provide some level of protection to forcible submergence.
Large numbers of stranded sea turtles are found to have man-made debris in their
digestive tracts. Turtles tend to ingest debris frequently, but in small amounts. Debris
of any size may be found throughout the GI tract. While all species of turtles have
been found to ingest foreign matter, Kemp’s ridleys seem to be less likely to do so
than other species.^^
Intestinal foreign bodies can harm the health of the turtle both directly and
indirectly. Plastics and other materials may block the intestinal tract, or cause local
necrosis and ulceration. Indirectly, ingested waste material may degrade the condi
tion of the turtle by interfering with lipid metabolism,increasing intestinal transit
time, or contributing to the accumulation of intestinal gas and uncontrollable floating.
Tar and crude oil may be ingested and act as a gastric foreign body, interfere
with intestinal function, and possibly cause serious toxicity problems (discussed
Health Problems and Diseases of Sea Turtles 379
further in the following section). Petroleum products can also cause external fouling
and act as a fonn of entanglement. According to stranding records, green turtles and
hawksbills are more apt to be impacted by tar and oil than other species.
14.9.2 A nesthesia
Sea turtles can be anesthetized with injectable or inhalation anesthetics. Ketamine
hydrochloride ( 1 0 ml at 1 0 0 mg/ml) mixed with acepromazine maleate ( 1 ml at 1 0
mg/ml) can be used as a stock solution (ket/ace) and administered intravenously
(IV) at a rate of 25 mg/kg of body weight. This dosage was developed for 10-kg
turtles and should be allometrically scaled^^ for use in larger turtles. Isoflurane alone
or in combination with nitrous oxide can be used to induce and maintain general
anesthesia. The turtles can be masked down with the inhalant anesthetics and then
intubated, or they can be administered ket/ace IV and intubated.
Depth of anesthesia can be monitored by assessing muscle tone and ocular blink
reflexes. In addition, if instrumentation is available, the heart rate can be followed
with an ultrasonic doppler or an electrocardiograph.
14.9.3 Resuscitation
Turtles that have become comatose from anoxic submergence, cold stunning, or an
adverse reaction to anesthesia can be successfully resuscitated.^^ Comatose turtles
Health Problems and Diseases of Sea Turtles 381
TABLE 14.1
Hematology and Blood Chemistry Values for Healthy, Wild, Juvenile Sea
Turtles
Lepidocheiys
Species Caretta caretta^ kempii^^ Cheionia mydas^^
Location Chesapeake Bay New York Bight Bahamas
Sample Plasma Serum Plasma
Number tested n = 50 tab n = 60 n = 100
Parameter Mean SD Mean SD Mean SD
* N = 106.
2 Aspartate aminotransferase.
^ Alkaline phosphatase.
Lactic dehydrogenase.
maintained at their optimum temperature (25 to 30°C) will revive faster than turtles
with lower body temperatures.
The first step should be to establish an open airway. After placing a bite block
into the animal’s mouth, a cuffed endotracheal tube should be inserted through the
larynx and into the trachea. The lungs can then be inflated by using a positive
pressure resuscitation bag. Care should be taken not to overinflate the lungs. Rather,
they should be gradually inflated until the carapace starts to rise. The turtle should
be ventilated several times per minute until the animal shows signs of reviving.
As the turtle starts to revive its heart rate will increase and it may move its front
flippers. Shortly after voluntary movement begins, the turtle will generally breathe
on its own. at which time the endotracheal tube should be removed.
382 The Biology of Sea Turtles
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'j C Human Impacts on Sea
Turtle Survival
Molly E. Lutcavage, Pamela Plotkin, Blair
Witherington, and Peter L. Lutz
CO NTENTS
0-8493-8422-2/97/$0 00+$.50
© 1997 by CRC Press, Inc 387
388 The Biology of Sea Turtles
15.1 IN T R O D U C T IO N
As detailed in the introductory chapter by Pritchard, sea turtles have a long and
ancient history. Sea turtles arose in the early Mesozoic, and for the next 100 million
years, during the rise and reign of the dinosaurs, they shared the oceans with a rich
diversity of other air-breathing marine reptiles. By the end of the Cretaceous era,
the sea-going ichthyosaurs and plesiosaurs were extinct, but sea turtles tenaciously
survived and indeed flourished up until very recent times. But today their numbers
are drastically reduced to the point that all seven remaining sea turtle species are
considered either threatened or endangered on a worldwide basis. Undoubtedly,
human interference is the cause of this collapse. The challenges that sea turtles now
face from human activities impact every stage of their life cycle, from loss of nesting
beach and foraging habitats to mortalities on the high seas through intense pelagic
fishing practices. They are also harmed by the increasing loads of nonbiodegradable
waste and pollutants that the oceans and coastal zones now receive.
Clearly, a full consideration of all human activities that adversely impact sea
turtles and required conservation measures would be beyond the scope of a single
chapter. However, a review of the more compelling effects will give an indication
of the extent of the challenges sea turtles face and the efforts required to protect
them. A word of caution: by its very nature much of the data on this subject is only
found in the “grey literature” of government and conservation organization reports
and such like, and is therefore scientifically unreviewed. This is sometimes the case
in conservation biology, where threats to survival are first recognized and conser
vation remedies proposed by biologists familiar with the status of local populations.
15.2 H A B ITA T A LT E R A T IO N A N D LO SS
The translocation of nests into hatcheries is probably the most common sea turtle
conservation technique undertaken when beach operations threaten sea turtles. These
efforts are usually practiced where serious threats to embryos or hatchlings are
thought to exist. High levels of egg poaching, predation, erosion, artificial lighting,
beach nourishment projects, and vehicular and foot traffic have been cited as justi
fication for nest translocation. However, there are potential problems inherent in
moving eggs and incubating them under artificial conditions, including increased
egg mortality and reductions in genetic and realized fitness.
Translocated sea turtle nests can have lower hatching rates than undisturbed
natural nests,although under certain conditions translocated nests may do better.^
Additional problems with translocation arise from changes in the incubation envi
ronment. Differences in temperature, hydric environment, and gas exchange affect
hatching rates and possibly the vigor and survivorship of hatchlings."^ During the
initial years of the Kemp’s ridley conservation program, low egg incubation tem
peratures resulted in the production of hatchlings that were primarily males.^ This
example shows that merely counting the number of mostly male hatchlings produced
does not reveal the success of a translocation program. Important assessments for
Human Impacts on Sea Turtle Survival 389
conservation include both number of hatchlings and their fitness — i.e., likelihood
of surviving, returning to an appropriate beach, and leaving offspring that themselves
survive.
Beach armoring consists of hardened structures meant to protect dune property from
erosion. This includes vertical or inclined concrete sea walls, wooden walls, rock
revetments, and sandbag/sandtube structures.^ From the perspective of a nesting
turtle, armoring is detrimental if it is rigid enough to impede access to the nesting
habitat. Even “soft” structures such as sand fences may pose threats similar to harder,
more pemianent fortifications. Structures meant to control longshore sand movement
such as groins and jetties also may present similar barriers to nesting turtles.
The greatest threat that armoring poses for sea turtles is to eliminate nesting
habitat. An example of how this can occur can be found in the case of the barrier
island nesting beach at Jupiter Island, FL. This partially developed island suffers a
net erosion of sand largely due to the sand starvation effects of an inlet Jetty. Whereas
both developed and undeveloped stretches of Jupiter Island are losing sand from the
beach, only the developed stretches have been armored. On the natural beach, dune
sand has replaced eroded beach, but on armored stretches, waves wash up to the
foot of walls and revetments, leaving no dry beach for nesting.
The problems caused by beach armoring will be greatest where coastal devel
opment is most widespread. This is especially the case in the U.S. and Mediterranean
where coastal development is older and dune structures have had more time to
become threatened by erosion. Elsewhere, the installment of armoring and its effects
on sea turtle nesting will become an important conservation issue as sea level rises,
beaches erode, and buildings are defended from the sea.
Whether sea turtles kept from nesting at armored beaches may forgo nesting
altogether or move to adjacent suitable nesting beaches is unknown. Although a
concentration of nesting effort at suitable nesting sites would allow reproduction to
continue, an increase in density-dependent mortality of eggs and hatchlings would
ensue.
that differ from a natural beach include sorting, moisture content, reflection, and
conduction. These properties affect the architecture of the egg chamber, incubation
temperature, gas exchange, and water uptake for a developing clutch and result in
diminished egg and hatchling survivorship.
In Florida, exhaustion of local sand sources for beach renourishment has stim
ulated the use of aragonite sand from the Bahamas. Incubation temperatures of
clutches in the white aragonite sand are 1 to 2°C lower than for natural Florida
silicate sands, a factor which may have an important effect on the hatchling sex
ratios (see chapter by Ackerman).^
There are economic incentives to nourish beaches during the summer nesting
season when costs are lower due to calmer seas. Renourishment projects during the
nesting season are already taking place in Florida, with an environmental cost to
sea turtles. Although large-scale translocations of eggs to hatcheries precede these
projects, an estimated 8% of the nests are missed by field workers and probably
destroyed during beach nourishment activities. Translocation itself can cause egg
mortality, and the process of nourishment involving lighting, sand spreading, and
pumping activities could disturb nesting females, and produce disorientation and
mortality in hatchlings.
In sand mining operations, large quantities of sand are dug from beaches to be
used as fill, in production of concrete, and for other construction activities. Sand
mining destroys the beach, eliminates nesting, and is recognized as a threat to some
sea turtle populations, primarily in the Mediterranean.^-^^^
Although the effects of human foot traffic on sea turtle nests are largely unstudied,
these perceived impacts are commonly used as a rationale for translocating nests.
As with vehicular traffic, foot traffic has the potential to damage buried eggs and
harm pre-emergent hatchlings. Human visitation at night can cause turtles to abort
nesting attempts, although where visitation is controlled within guided “turtle watch”
groups, this disturbance is m i n i m a l . “Turtle watch” outings have an important
Human Impacts on Sea Turtle Survival 391
conservation and education potential and are becoming a common forni of eco-
tourism. However, even well-intentioned eco-tourism programs can lead to unex
pected conflicts. For example, in Costa Rica, former subsistence turtle hunters and
“hueveros”, or egg collectors, have been trained as turtle guides, and now derive
income by leading nesting beach tours. There is some indication that the strong
economic incentive to attract a large number of tourists to some important nesting
beaches may be at odds with the need to minimize disturbance to nesting females
and emergent hatchlings. Fortunately, these problems can be addressed through
educational programs and goal setting by the conservation and residential commu
nities alike.
Sea turtle stranding data for the U.S. Gulf of Mexico and Atlantic coasts, Puerto
Rico, and the U.S. Virgin Islands show that between 1986 and 1993, about 9% of
living and dead stranded sea turtles had propeller or other boat strike injuries (n =
16,102).^^’^^ In Florida where coastal boating is popular, frequency of boat injuries
between 1991 and 1993 was 18% of 2,156 strandings. Countless numbers of log
gerheads and 5 to 50 Kemp’s ridley turtles are estimated to be killed by vessel traffic
per year in the U.S.^^ Although some of these strikes may be post-mortem, the data
show that vessel traffic is an important cause of sea turtle mortality.
Dredge and fill activities occur in many of the nearshore seasonal habitats of sea
turtles worldwide. Operations range in scope from incidental propeller dredging by
recreational boats to large-scale dredging of channels and fill for land reclamation.
Sea turtles have been killed by hopper dredging in Florida and Georgia, but the
extent of mortality in other geographic regions is unknown.^^
Sea turtles associate with offshore oil and gas platforms, and can be hamied by
underwater explosions detonated to remove platforms no longer in service.^^ These
explosions can cause capillary damage, disorientation, and loss of motor control in
sea turtles. Although sea turtles far from explosion sites may suffer only disorien
tation, those near detonation sites will most likely sustain fatal injuries.^® Estimates
of sea turtles killed from explosive platform removal in the Gulf of Mexico range
from 50 to 500 per year.^^ Petroleum seismographic canons and military maneuvers
involving explosives also have the potential to harm sea turtles, but specific infor
mation on their impacts is lacking.
Companion animals and pests attracted to trash and food remains left by humans
can have a devastating impact on the success rates of sea turtle nesting beaches.
Nesting depredation by feral hogs, pigs, foxes, coyotes, coatis, mongooses, and dogs
can destroy up to 100% of nests.Municipalities have taken steps to eliminate or
reduce this disturbance with predator removal programs and the use of tamper-proof
trash receptacles.
15.3 O IL P O L L U T IO N
Sea turtle populations around the world coexist with oil and gas exploration, trans
portation, and development. The risk of this association is demonstrated in the
growing record of turtles harmed by oil in the Gulf of Mexico,^ ^ the Atlantic,^^ the
Caribbean Sea,^^ the Gulf of Iraq,^^ the M editerranean,and the Red Sea.-^^’ Indeed,
Human Impacts on Sea Turtle Survival 393
the extent of oil and tar pollution in ocean surface waters is enormous. The Sargasso
Sea alone entraps an estimated 70,000 metric tons of tar.^^
Sea turtles are exposed to the hamiful effects of oil pollution in many ways
(Figure 15.1). By continuously resurfacing through an oil slick to breathe, adults
would experience prolonged physical contact with the floating oil. Ocean convergent
zones and drift lines, where pelagic juveniles are found, are also regions where
floating tar accumulates. The sea turtle’s mode of respiration, a rapid inspiration of
surface layer air before diving, would introduce petroleum vapor into the lungs.
Eating contaminated food or tar balls brings petroleum materials into the intestine.
On nesting beaches, oil deposits could interfere with normal development of the
embryos in the egg clutch as well as present a lethal hazard to newly emerged
hatchlings. It is clear that all sea turtle species and life stages are vulnerable to the
harmful effects of oil, through direct contact or by fouling of their habitats.
ROUTE RESULT
Carcinogenesis — DEATH
SKIN
Parasitism, Disease ► Viability — PREDATION
Decreased INCREASES
^Aerobic scope
LUNG decreased Foraging time
"reduced
Dive time
decreased GROWTH
REDUCED
^Assimilation
GUT decreased
, Organ ---- PERFORMANCE
Internal effects - Dysfunction DEPRESSED
Hormone ----------- ^ REPRODUCTION
Balance disturbed DECREASED
SENSE - Interference BEHAVIOR
ORGANS PERTURBED
EGGS - Abnormal
Development DEATH
FIGURE 15.1 Conceptual model of potential effects of prolonged oil exposure on sea
turtles. The primary impacts on organ systems, drawn from documented effects in laboratory
studies, may work synergistically to produce behavioral changes, interference with sensory
systems, reduced fitness, and eventual death. (From Lutz et al.^^)
There is direct evidence that sea turtles have been seriously harmed by oil spills.
A recent fuel oil spill oiled loggerhead nests and jeopardized hatchlings and adult
females in Tampa Bay, FL. In 1979, the Mexican Ixtoc blowout oil breached Rancho
Nuevo, the only known major nesting beach of the Kemp’s ridley, and oiled sea
turtles were subsequently seen in Texas coastal wa tersThere is evidence that in
1983, 180 hawksbill turtles were killed by oil spilled off the islands of Jana and
Karan, near Saudi Arabia."^^ Although the documentation of the numbers of sea turtles
394 The Biology of Sea Turtles
killed by oil spilled during the Gulf of Iraq conflict is scanty, estimates range from
tens to hundreds.'^^
On a yearly basis about 1% of strandings identified by the U.S. sea turtle
stranding network are associated with oil, with higher rates in South Florida (3%
of strandings) and Texas beaches (3 to 6.3% of s t r a n d i n g s ) . F r o m 1980 to 1984,
37.5% of Dade County, FL sea turtle strandings were petroleum related, probably
due to the heavy surface and beach tar occurrences reported in South Florida."^^ Rates
may also be high in transport routes through the Caribbean and other areas. Oil
removed from stranded sea turtles in Florida and Texas has been identified primarily
as tanker washings from diverse sources."^^ Pelagic size-class loggerheads, hawks-
bills, and green turtles are known to strand, having been mired in oil.^^
In the western Atlantic and Gulf of Mexico offshore, sea turtle distribution has
been linked to zones of convergence.^^-^'^ Convergence fronts and Langmuir cells
concentrate zooplankton and other prey items, serving as dependable feeding zones
for sea turtles, marine birds, and other pelagic foragers. Unfortunately, marine debris
and oil also collect in these areas, which increases the probability of prolonged
contact with oil, tar, and harmful d e b r i s . I n convergence fronts east of Florida,
tar was found in the mouth, esophagus, or stomach of 65 out of 103 post-hatchling
loggerheads."^^ A loggerhead sea turtle sighted during a Gulf of Mexico aerial survey
surfaced repeatedly within a surface oil slick for over an hour."^^
Dramatic as it is, direct evidence of the effects of oil on sea turtles must grossly
underrepresent the problem. Oiling on the high seas and inaccessible beaches are
not likely to be documented. Secondly, only a small percentage of the animals killed
by oil would presumably strand, and of these, even fewer are likely to be found and
recorded.
Although lethal effects of oil pollution on sea turtles have been demonstrated, e.g.,
hatchlings apparently starved to death as a result of their beaks and esophagus being
blocked with tar balls, sublethal encounters are likely to be much more common,
and may have a greater deleterious impact on sea turtle populations. Laboratory
studies indicated that sea turtles will pursue and swallow tar balls indiscriminately
and that they appear to be unable to detect and avoid oil slicks."^^
Despite the high tolerance of sea turtles to physical damage, they are surprisingly
sensitive to oil. In laboratory studies on juvenile loggerhead turtles, almost all of
the major physiological systems were adversely affected by short exposures to
weathered South Louisiana crude oil.^^’^^ Significant changes in respiration, diving
patterns, energy metabolism, and blood chemistry occurred following oiling. A three-
to sixfold increase in white blood cell count suggested that a rapid immune response
was initiated in oiled turtles. Hematocrit and hemoglobin concentration decreased
slightly during oiling. These parameters are critical components of the oxygen trans
port system of the blood. Changes in metabolic indicators showed temporary inter
ference with digestion. The salt glands, paired organs located in the eye orbits (see
Chapter 13), failed to produce fluid several days after oil exposure, although function
returned within two weeks.^^’^^ Mechanical blockage of tear ducts or an inflammatory
Human Impacts on Sea Turtle Survival 395
response to the presence of oil may have inhibited salt gland function. Prolonged
salt gland failure could have serious if not fatal consequences, since it would interfere
with water balance and ion regulation. Turtles also experienced acute contact der
matitis, or disruption of the architecture of the skin, mucous membranes, and sensory
o r g a n s . A break in the skin barrier could act as a portal of entry for pathogenic
organisms, leading to infection and debilitation.
Petroleum hydrocarbons were not detected in skin or blood samples from log
gerhead sea turtles that were exposed to a weathered oil slick for no more than 96
However, there is some evidence of bioaccumulation in sea turtles exposed for
longer periods. After the Gulf of Iraq war, a stranded green sea turtle had 4050 and
310 mg of oil per kilogram of tissue in its liver and stomach, respectively."^^ Externally
the turtle did not appear to have been oiled, and until necropsy had been assumed
to have died of natural causes.
Kemp’s ridley sea turtle embryos from eggs incubated in sand contaminated in
situ by the Ixtoc blowout had survival rates similar to eggs incubated in unoiled
sand."^^ However, Ixtoc oil was highly weathered, and would have lacked most of its
volatile components. In contrast, the number of viable eggs and hatchlings surviving
to be released from nests incubated in the laboratory were significantly lower in
nests contaminated by unweathered crude oil than that of controls.^® It should be
emphasized that the laboratory studies only concerned the effects of weathered oil
— prolonged contact with freshly spilled oil would undoubtedly be considerably
more harmful. It stands to reason that the cumulative harmful effects of an oil spill
can be catastrophic.
Oil-spill response activities have great potential to harm sea turtles if earned out
without regard to sea turtle behavior. Beach cleaning operations involve artificial
lighting and heavy machinery which may disturb the beach profile and disorient
nesting sea turtles and hatchlings. Because of the timing of critical events during
embryogenesis, sea turtle eggs can only be moved during specific periods of incu
bation, which limits relocation as a mitigation option.^®’^^
Booms used to contain oil may impede access, cause direct fouling or entangle
ment, and increase predation risks to hatchlings. Increased vehicular and vessel
traffic in shallow areas such as seagrass beds can adversely affect foraging habitat
and cause displacement from preferred areas. Oil spill contingency plans for areas
where sea turtles are found should have specific response procedures in place to
protect sea turtles.
15.4 O T H E R P O L L U T IO N S O U R C E S A N D
E N TA N G LE M EN T
Sea turtles can achieve life spans greater than 50 years, and have a potential to
bioaccumulate heavy metals and pesticides. In the coastal zone, chronic pollution
from industrial, agricultural wastes, and urban runoff is a threat to sea turtles.^^’^"^’^^’^^
Unfortunately, there is little information about this problem. In Chesapeake Bay,
396 The Biology of Sea Turtles
DDT and its metabolites DDE and DDD were found in gonad, fat, liver, and muscle
tissues of loggerheads and Kemp’s ridleys.^"^ In some marine mammals, chronic
pollution from industrial or agricultural sources has been linked with immune sup
pression, raising a concern for sea turtles. Marine turtle fibropapilloma disease is
currently associated with a viral infection (see Chapter 14); however, the expression
of the disease may be mediated by a compromised immune system (Figure 15.2).^^
FIGURE 15.2 A hawksbill turtle found entangled in plastic debris and an onion wrap.
Much of the marine debris that entangles sea turtles originates from boats, ships, fishing
vessels, and offshore oil and gas platforms. (Photo courtesy of Anthony Amos, University of
Texas; Marine Science Institute, Port Aransas, Texas.)
15.4.2 Ingestion
Reports of debris ingestion exist for almost all sea turtle species and life stages.
However, the dependence of pelagic juveniles upon convergence zones, where
Human Impacts on Sea Turtle Survival 397
floating debris concentrates, and the indiscriminate nature of their feeding strategy
leave pelagic turtles most susceptible to debris ingestion.^^’^"^ Adult and subadult
leatherback and loggerhead turtles utilize convergence fronts for travel routes.
Unfortunately, they too are at increased risk of ingesting and becoming entangled
in debris.
Marine debris is also prevalent in nearshore habitats, and there are numerous
reports documenting the occurrence of marine debris in the digestive tracts of post
hatchling and juvenile loggerheads,^^ green tuitles,^^ l ea th erb ac ks, K em p’s rid-
leys,"^"^ and hawksbill turtles.
Debris ingestion by sea turtles appears to be high in some regions and low in
others. For example, 32% of post-hatchling loggerheads captured off the east coast
of Florida had ingested plastics and synthetic fibers,and high frequencies of debris
ingestion has been reported for juvenile sea turtles in the northwestern Gulf of
Mexico^^’^^ and on the Gulf and Atlantic coasts of Florida.^"^ In contrast, low fre
quencies of debris ingestion were reported for Kemp’s ridleys, loggerheads, and
green turtles in the New York Bight.^^ Loggerhead and leatherback turtles apparently
ingest more debris than other species.The benthic foraging strategy of loggerheads
in nearshore habitats, where debris accumulation may be quite high, lends itself to
increased chance of encounter with debris. Leatherbacks are pelagic foragers that
prey on cnidarians and other gelatinous invertebrates, and it has been suggested that
they may not distinguish between their gelatinous prey and transparent plastic, the
type of debris most often present in their digestive tracts.
The list of materials found in turtle digestive tracks is extraordinary. Plastic bags,
sheeting, beads, pellets, line, rope, strapping, pieces from bottles, and hard pieces
of unknown origin are common items ingested by sea turtles.^^ '^'^ Sea turtles have
also ingested latex balloons, aluminum, paper, cardboard, styrofoam, rubber, string,
cigarette filters, wax, cellophane, fish hooks, charcoal, and g l a s s . I n d e e d , it is
possible that hungry sea turtles, particularly loggerheads, will swallow almost any
material of a suitable size and consistency and will continue to do so until satiated.
Debris ingestion is not often directly implicated in turtle mortality, namely because
only small quantities of debris well mixed with natural food items are generally
seen in the digestive tract."^"^’^"^’^^ Debris in the digestive tract is commonly seen during
necropsies^^ and is defecated by live captured sea t u r t l e s . I s the ingestion of
debris, in particular plastic and latex, by sea turtles therefore any cause for concern?
Clearly, if sufficient material is swallowed to cause a complete stoppage of the gut,
death will result from starvation. This appears to be a relatively rare event in
comparison with the frequency with which smaller amounts are ingested.However,
the situation is much more serious if sea turtles are harmed by ingestion of plastics
and latex at quantities much smaller than those necessary for gut strangulation.
Controlled studies where turtles were presented with small amounts of debris
demonstrated that when hungry, sea turtles will actively consume plastic and latex
material.^®’^^ The sojourn of the ingested latex material in the gut ranged from a few
days to four months, indicating that some of the latex pieces were being held up in
398 The Biology of Sea Turtles
the intestine. Although little change in blood chemistry was noted following inges
tion in these experiments, except for a fall in blood glucose levels, some turtles
became positively buoyant.^® '^^ In the wild, positive buoyancy would render afflicted
individuals more susceptible to collisions with boats, increase the chance of inci
dental capture in some commercial fisheries, and increase predation risk.^^ Clearly,
information is needed on the physiological effects of ingestion of larger quantities
of plastics by sea turtles and the levels at which adverse impacts occur.^^ It has also
been suggested that ingested plastic could result in PCB accumulation.^"^
1 5 . 4 .4 Entanglement
Entanglement in derelict fishing gear is a serious problem for sea turtles. Entangle
ment may occur when sea turtles accidentally swim into the derelict fishing gear or
when they are attracted to the accumulated epibionts encrusting old floating debris.
Leatherbacks have been reported entangled in active crab and lobster traps and even
whelk lines,^^’^^ and there are records of juvenile hawksbills trapped in plastic-woven
sacks.Entanglement can result in reduced mobility, making the turtle unable to
feed or flee from predators. If forced to remain at the surface they may become more
susceptible to collision with boats or incidental capture. Trailing debris may trap
sea turtles between rocks or ledges, resulting in death from drowning, and constric
tion of the neck and flippers can amputate limbs and consequently lead to death
from infection.
Much of the marine debris that entangles sea turtles originates from boats and ships
and from offshore oil, gas, and chemical platforms. Some marine debris comes from
land, entering the oceans via rivers and beaches. Plastics are the most abundant type
of anthropogenic debris found on beaches and in the oceans.^^ Plastics are also the
most common type of debris sea turtles become entangled in and ingest.^^ As the
general use of plastics for industrial and commercial purposes increased over the
last 40 years, so have the amounts and types of plastic appearing in the oceans.
Fishing gear, and in particular monofilament line, is one of the most commonly
encountered anthropogenic debris that endangers sea turtles, and may account for
68% of all entanglement cases.^^’^^ In addition to material commonly perceived as
gear, plastic-fiber bags, sheeting, strapping, cloth, burlap bags, plastic 6-pack yokes,
steel cable, aluminum beach lounge chairs, and polypropylene rope are also impli
cated.
15.5 F IS H IN G A N D IN C ID E N T A L C A P T U R E
In a comprehensive review of sources of mortality in sea turtles conducted by the
National Academy of Science, incidental capture in shrimp trawls was said to account
for more deaths than all other sources of human activities combined.^^ Before the
implementation of protective measures, direct mortality of an estimated 5,000 to
50,000 loggerheads and 500 to 5,000 Kemp’s ridleys was believed to occur yearly
Human Impacts on Sea Turtle Survival 399
in U.S. waters as a result of drowning in shrimp trawls.Sea turtles have also been
killed when incidentally captured in purse seine, gill nets, and various types of
untended fishing gear. Lobster and crab pots and hook and line fishing also cause
entanglement, mutilation, and debilitation. The mortalities associated with these
fishing categories are estimated to be about 10% of that attributed to shrimp trawl-
ing.2’
Because sea turtles are exceptional breath-hold divers, there was initial skepti
cism about the estimated numbers of turtles caught and killed by inadvertent capture
in trawls. This was shortly cast aside by a series of field and laboratory studies
examining the impacts of enforced submergence. A study examining the relationship
of tow time and sea turtle mortality showed that mortality was strongly dependent
on trawling duration, with the proportion of dead or comatose sea turtles rising from
0% for the first 50 min of capture to 70% after 90 min capture.^"^ Loggerhead turtles
captured in shrimp trawls with tow times of less than 30 min showed severe acidosis,
and acid-base recovery times took as much as 20 h.^"^ In laboratory and field studies,
forcibly submerged loggerhead, green, and Kemp’s ridley sea turtles suffered sub
stantial acidosis within 30 min, and also required many hours to restore acid-base
b a l a n c e . T h e physiological disruptions associated with enforced submergence
(see Chapter 10) would be sufficient to cause a comatose state leading eventually
to death.^^ Sea turtles forcibly submerged in any type of restrictive fishing gear would
eventually suffer fatal consequences from prolonged anoxia and/or seawater infil
tration of the lung.
In 1978, the U.S. National Marine Fisheries Service and Sea Grant Program under
took development of trawl modifications (turtle excluder device, or TED) that would
allow captured sea turtles to escape the trawl through an escape hatch (Figures 15.3
and 15,4). By 1987, regulations required seasonal TED use in offshore shrimp
trawlers from North Carolina to Texas, although these were not finalized and fully
enforced until September 1989. Following years of unsuccessful voluntary compli
ance programs, legislation was passed in 1994 requiring year-round use of certified
TEDs by U.S. shrimpers from North Carolina to Texas. The use of TEDs in South
Carolina waters is believed to have reduced annual loggerhead strandings by 44%.^^
Repeated capture by trawlers in intensely fished areas may cause some sea turtle
mortality (despite the use of TEDs) in cases where sea turtles are unable to com
pensate for repeated metabolic stress.
Because of the high product value, shrimping intensity is high in coastal regions
worldwide.^'^ Given the highly migratory nature of sea turtles, protective measures
on the part of one country would shortly be undone by unrestricted fishing practices
of adjacent countries. In order to conserve the sea turtles, mitigation of incidental
catch must be addressed by all shrimp-fishing nations. In 1993, Mexico required
that offshore trawlers use TEDs in Atlantic and Caribbean fishing zones. The concept
and application of the TED has expanded, and these devices are now known as
“bycatch excluder devices” in recognition of their value in reducing undesired
bycatch of finfish by as much as 50 to 70%.^^ The U.S. National Marine Fisheries
400 The Biology of Sea Turtles
FIGURE 15.3 A loggerhead sea turtle shown escaping from a trawl net via the turtle
excluder device (TED). (Photo courtesy of NOAA, National Marine Fisheries Service, Mis
sissippi Laboratories, Pascagoula Facility, MS.)
Service is currently providing bycatch and TED advisory services to fishing nations
of the Pacific Rim and Indian Ocean.
Sea turtles are also vulnerable to capture in pelagic longline, paired trawl, and gill
net fisheries, a source of increasing concern for both fisheries managers and the
industry. Incidental sea turtle take on swordfish and tuna fishing grounds had been
noted since the 1950s, yet more recent information suggests that interactions may
be higher than acknowledged. The pelagic swordfish fishery has shifted focus since
the late 1980s, encompassing wider fishing areas in the Atlantic and Caribbean, and
new gear types such as paired trawls. Estimated total take of sea turtles in the
Northwest Atlantic for 1989 to 1992 was 1218 turtles.From 1991 to 1993, NMFS
observers on 54 pelagic longline trips targeting swordfish, tuna, sharks, and mixed
species recorded 56 leatherbacks and 29 hardshelled or unidentified sea turtles.In
the Hawaiian longline tuna fishery,^^ leatherback captures were documented from
January to December 1992.^^
This pattern of high incidental catches may have been common in U.S. waters
since the opening of the fisheries, and appears to be selective for certain species.
WitzelE^ estimated that 79.6% of the observed take (57 sea turtles) of the Japanese
tuna longline fleet in the Gulf of Mexico and Atlantic (1978 to 1981) were leather
backs. Lethal encounters appear to be higher for chelonid sea turtles, usually found
impaled on the hook.^^’^"^’^^ Leatherbacks are usually found alive, but fouled in
longline gangions (drop lines) or buoy lines.^^’^'^Longlines are soaked after sunset,
Human Impacts on Sea Turtle Survival 401
FIGURE 15.4 A schematic showing an example of a recent turtle excluder device. A variety
of approved TED designs are available to maximize exclusion of sea turtles and bycatch under
different fishing conditions. (Schematic courtesy of NOAA, National Marine Fisheries Ser
vice, Mississippi Laboratories, Pascagoula Facility, MS.)
and chemical lightsticks are commonly used to lure target species to the bait.
Leatherbacks may be attracted to the lightsticks, or medusae fouled on longline
sections, whereas chelonid species such as loggerheads, greens, and Kemp’s and
Pacific ridleys appear to seek baited hooks. Even if successfully freed from longline
gear, sea turtles may suffer subsequent mortality from hook wounds, internal bleed
ing, or entanglement.
In recent years the documentation of sea turtles in coastal regions and their incidental
take by inshore fisheries has received considerable attention. Collectively, unattended
nets set in shallow waters and fisheries other than shrimping are the second largest
source of mortality to sea turtles.Coastal fishing practices including pompano gill
nets and trammel nets for shark and bluefish (Atlantic Florida), summer flounder
trawling (southern Virginia to North Carolina), shark and sturgeon nets (Georgia,
South Carolina), and pound nets and weirs (Atlantic coast) also have incidental takes
of sea turtles.^^’^^’^^ Sea turtle mortality associated with these fisheries varies in
response to the seasonal abundance of turtles and to the intensity and timing of
fishing effort. The short pulses of high mortalities associated with some of these
fisheries may disproportionately impact highly depleted species such as the Kemp’s
ridleys. Gear modification and short-term closures are being evaluated as mitigating
measures for some fisheries.
402 The Biology of Sea Turtles
If we are to protect sea turtles from further population decline we clearly need
to encourage the development of fishing techniques that minimize or eliminate
incidental take. This task is difficult to achieve until we understand the nature of
sea turtle migration and foraging routes, particularly in offshore areas where migra
tion routes are poorly known. Incidental take records and anecdotal observations
from the fisheries document a notable abundance of sea turtles on shelf break (200-
m depth contour) or at edges of oceanic gyre systems such as the Gulf Stream
western boundary and Gulf of Mexico Loop Current.^'^’^'^’^^’^'^’^^’^^ New technologies
such as satellite tracking, remote sensing of the environment, and other techniques
may help quantify the types and degrees of interaction of sea turtles with human
activities on the high seas. Observer programs and better recordkeeping by fishermen
may also help quantify the impacts of fishing and offer solutions for mitigation
efforts.
The worldwide decline of nearly all sea turtle populations can be attributed in part
to the direct take of turtles and their eggs by commercial and subsistence sea turtle
fisheries. Particularly in developing countries, the need for protein and income
generated by sea turtles and their by-products greatly exceeds any desire for their
preservation. Although all sea turtle life stages have been exploited, perhaps with
the exception of hatchlings, breeding adults and their eggs have been most vulner
able. Nesting females and the eggs they leave buried in the sand have provided easy
access to an otherwise difficult to reach resource. Mating areas and nearshore feeding
grounds, particularly reefs frequented by hawksbills, also provide relatively easy
access to large numbers of sea turtles.
In many cultures, sea turtle eggs are eaten not only for nutrition, but also because
of puiported aphrodisiac qualities. Green turtles are sought after primarily for their
meat and cartilage (calipee), as well as their shells and bodies, which are sometimes
stuffed and sold as curios for the tourist trade. Even bones are used to make
houseware items, decorative artwork, ornaments, jewelry, and fertilizer.Pacific
ridley skin is used to fashion boots, shoes, handbags, and other accessories, and oil
rendered from the carcass is used for everything from skin lotion additives to boat
caulking agents. Hawksbills are highly desired for their beautiful shells, which are
polished and hung on the wall as a decorative ornament, or the scutes from their
shells (“bekko”) used to craft jewelry, combs, and eyeglasses.
Since prohibited by the Endangered Species Act of 1973, commercial sea turtle
harvests no longer exist within continental U.S. waters, and were recently banned
in Mexico.^^ Sea turtle fisheries, nonetheless, continue elsewhere. Since the mid-
1970s, the primary importers of sea turtle shell and skin have been Japan, Hong
Kong, China, Singapore, Taiwan, and some European countries.^^^^^^’^^ The major
exporters have been Panama, Honduras, Jamaica, Haiti, Cuba, Cayman Islands,
Maldives, Fiji, Solomon Islands, Indonesia, Philippines, Thailand, Kenya, and Tan
zania.^®These countries generally harvest only turtles from their beaches and
territorial waters, and in many instances feel their activities are justified because
they exploit their own resources. But in reality, sea turtles are highly migratory
Human Impacts on Sea Turtle Survival 403
species that, on a geographic scale, are a regional resource to be managed and shared
among many countries.
Commercial exploitation has resulted in overharvesting and in declines or local
extinctions of the populations harvested. Unfortunately, the effects of a commercial
harvest are not immediately apparent and may give the false impression that con
tinued exploitation will not be detrimental to the population being harvested. This
is best illustrated by the Pacific sea turtle fishery in Mexico which harvested hundreds
of thousands of olive ridleys annually over several decades. This lead to the demise
of at least three local nesting populations and a precipitous decline of the species
in the eastern Pacific Ocean.
Legal and illegal subsistence sea turtle hunts and egg harvests occur worldwide,
but in contrast to commercial sea turtle fisheries, they supply local markets or tribes,
and in some instances the international tourist trade.^^ The level of subsistence take
and the corresponding threat to a particular population is difficult to determine
because the number of sea turtles and eggs taken generally is not documented or
reported. In some regions subsistence hunts are controlled by annual quotas and
appear to pose little threat to the resource.In other areas where quotas are nonex
istent, indigenous peoples have long ago exceeded the sustainable level of take and
have extirpated sea turtle populations.
15.5 C O N C L U S IO N S
There is ample evidence that human activity is seriously eroding once abundant sea
turtle populations. Much of the impact is unintentional, a consequence of the
increased exploitation of marine and coastal waters — the more intense fishing effort
results in an increase in the incidental catch of sea turtles and a higher risk of
entanglement in discarded gear. Debris and pollution in coastal areas take their toll
year after year. The inexorable spread of beach development eats away at natural
sea turtle nesting habitats and deters nesting females. Even well-intentioned eco-
tourism programs, which seek to educate visitors to turtle nesting beaches and offer
alternative employment for subsistence turtle hunters, have the potential for harm.
Poorly regulated foot traffic, noise, and lights create disturbances that may deter
nesting females and threaten hatchlings. There are also deliberate threats to sea turtle
survival, a consequence of both local economic pressures and international commer
cial interests which drive continued (even if illegal) fishing for juveniles and adults
and a massive taking of eggs.
In order to be biologically effective, conservation programs must be firmly based
on knowledge of how and to what extent humans are jeopardizing turtle survival.
In order to be accepted both politically and practically, conservation programs must
also recognize the economic forces behind the disturbing influences. These will be
different in different countries and even in different regions, so that implementable
solutions are/will be complex. Nevertheless, action is urgently need to halt the
decline and turn the situation around. Otherwise, the extinction of local populations
and even some species is inevitable.
404 The Biology of Sea Turtles
R EFER EN C ES
1. Pritchard, P. C. H., Why nest relocation programs may be harmful to sea turtles, in
P ro c. 5 th A n n u a l N a t. C o n f. o n B e a c h P r e s e r v a tio n T e c h n o lo g y , Tait, L. S., Ed.,
Florida Shore and Beach Preservation Association, Tallahassee, FL, 408, 1992.
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Index
Amnion, 66
Amniotic sac, 89-90
A a p to s , 205 A m u s iu m ja p o n ic u m b a llo ti, 212
Absolute growth rates, 241, 254, 256, 303 Amydae, 6
Absorptivity, 301 A n a d a r a tr a p e z ia , 204
Abundance, 261, 262 Anaerobic adaptations and migratory swimming,
A c a n th u r u s , 213 192-193, 288-290
Acepromazine maleate, 380 Anaerobic metabolism, 192, 288-290
Acid-base balance, 288, 353-357 Anapsid, 2
ACR (air convection requirement), 283, 356 A n c o r in a , 205
Adults, 139-140 Androgen, 316
homiones, 317-319 Anemia, 366, 375
migratory swimming, 186-188, 190-192 A n e n o m ia s u lc a ta , 205
reproduction, 52-65 Anesthesia, 380
A e q u o r e a , 209 Annuli, time intervals for deposit, 238-239
Aerial surveys, 152 Anoxic tolerance, 289-290, 378
Aerobic metabolism, 190, 284—288 Anthropogenic effects. S e e a ls o Pollution; Shrimp
Aerobic scope, 189, 281 trawls
A e r o m o n a s , 368 effects of fishing gear, 398, 399-401
A e r o m o n a s h y d r o p h ila , 367, 368 entanglement, 378, 396, 398
Affinities, of recent species, 13-16 on foraging ecology and nutrition, 218-221
Age ingestion of marine debris, 218-219, 378-379,
estimated, 248-249 396-398
known, 247-248, 250-251, 253 loss of habitat, 99-103, 388-392
reproduction and, 58 Antiperistalsis, 218
Age-based growth models, 241-242, 243-245, Antitropical distribution, 20
247-251 A p o le m ia u v a r ia , 209
Age-specific reproduction axioms, 256 Aquatic locomotion, 171-181, 183-184. S e e a ls o
Age-structured models Swimming
dynamic, 263-264 aerobic adaptations, 189-192, 284-288
static, 262-263 anaerobic adaptations, 192-193, 288-290
Air circulation, 301 Aragonite, 66
Air convection requirement (ACR), 283, 356 A rc h e lo n , 8, 10
Air temperature, 304 Archie Carr Center for Sea Turtle Research, 44
Alabama, L e p id o c h e ly s k e m p i, 149 A r e n a e u s c r ib r a r iu s , 207
Albumin, 381 A r g illo c h e ly s s u b s c r ip ta ta , 11
Algae. S e e a ls o S a r g a ss iim Arginine vasotocin (AVT), 327, 328, 335
as food resource, 140, 144, 145, 200-209, 210 A r g o p e c tin ir r a d ia n s , 207
v¿’. seagrass diet, 210-211 A r iz o n a , 367
Allantois, 66 Aromatase, 316
A llo p le u r o n , 7, 8 Arom gene, 316
A llo p le u r o n h o jfm a n n i, 11 Arrhenius equation, 85-86
304
A lp h a H e lix , Arribada beaches, 22, 59
Altered beaches, 99-103, 388-392 Artificial lighting, 116-117, 388, 391
Alternating gait, 59 Ascension Island, U.K.
Amebiasis, 375-376 C h e lo n ia a g a s s iz i, 15
Ammonia, 353 C h e lo n ia m y d a s , 17, 18, 38, 39, 126, 129
411
412 The Biology of Sea Turtles
immunosupression due to stress, 364, 370,374, stress and predisposition to disease, 364, 370,
396 374, 396
from ingestion of marine debris, 219, 220, temperature and the, 364, 370
378-379 Immunological data, 3 1
iron deficiency, 366 Imprinting
malnutrition, 365 chemical imprinting hypothesis, 126-128
metabolic bone disease, 365-366 corticosterone and, 317, 318, 336
mycotic diseases, 369-370 In a c h u s , 205
parasites, 364, 368-369, 374, 375-377 Incidental capture. S e e Fisheries; Shrimp
viral diseases, 237, 253, 289, 370-371 trawls
Heat energy exchange, 298, 301 Incidental drinking, 345-346
Heat gain, 311 Inclination compass, 119
Heat lag, 303, 307 Inconel, 237, 254
Heat load, 300, 301, 305 Incubation, 83, 84-85
Heat sink, 303 temperature during, 85-89
Heat storage, 298, 299 water storage in, 89
Heat stress, 299, 304 India, 22, 201
Heat transfer, 298, 299, 306 Indian Ocean, 20
Helminths, 376-377 Infections, 366-369
Hematocrit, 286 Infertility, 66
Hematology, 379-380 Ingestion
Hemoglobin, 287-288 of food, 213-214, 345
H e p a tiis e p h e litic u s , 207 of marine debris, 218-219, 378-379, 396-398
Heron Island, Australia. S e e a ls o Great Barrier Instantaneous energy exchange, 300
Reef Insulation, 311
nesting locations, 38, 200, 267 Intake, 213-214
thyroid study, 333 Interhabitat dispersal rates, 236
turtle diet, 210 Internesting period, 63
Herpesvirus, 370-371 Intracaidiac shunting, 282
Hibernation, 289-290, 333, 336, 355 Ionic regulation, 347-353
H ip p o c a m p u s e re c tu s , 204, 207 Iron deficiency, 366, 375
H o lo th w ia c u h a n a , 205 Islands and archipelagos, 154
Home range, 146, 148, 151 Isoclinics, 129
Homing. S e e Natal beach homing Isodynamics, 129
Hopper dredging, 392 Israel, 95
Hormones, 56, 289, 315-337, 334, 335 Iteroparous life cycle, 52, 259
Human impacts. S e e Anthropogenic effects lUCN (World Conservation Union), 16, 18
Humidity, 85
H y a la e a triclen ta ta , 203
Hybrids, 36-37, 45 j
Hydric conditions, 67-68 J a n th in a , 201, 202, 203, 208
H y p e r ia g a lb a , 209 Japan, 20
H y p e r ia m e d u s a ru m , 202, 203, 209 C a r e tta c a r e tta , 42, 43, 126, 142
Hyperthermic stunning. S e e Cold tortoiseshell imports to, 21
stunning turtle diet, 201
H y p n e a c e r v ic o r n is , 201 Jellyfish. S e e a ls o Food resources, jellyfish
nutritive value of, 214
salt load and, 351, 357
I Jurassic Period, 2
Ichthyosaurs, 3 Juveniles, 139
203
I d o te a m e ta llic a , early nursery habitat, 140-146
Iguanas, marine, 3 hormones, 317-329
Immigration rate, 261 later development habitat, 144, 146-153
Immune system orientation of, 124
effects of oil exposure, 394 thermal biology, 308
422 The Biology of Sea Turtles
Secondary sex characteristics, 321-322, 324, 335 heat transfer at the surface, 302
Secretory tubules, 347-348, 352 temperature, 299
Selvagens Islands, 203 water loss through, 345
S e m e le , 202 Slope, 110
Sensitivity analysis (elasticity), 264, 265, 266, Social facilitation, 38, 68
267 Sodium
Sensory deprivation experiments, 110 in plasma, 344, 381
Septicemia ulcerative cutaneous disease (SCUD), in salt gland fluid, 345, 349, 350, 351
368 in seawater, 344
Sequential population analysis (SPA), 262-263, in urine, 350
266 Soft-shelled turtles, 3, 6
Sexing technique, 319-321 Solar radiation, 298, 303, 306, 311
Sex ratios, 319-321, 335 S o le n g r a n á is , 204
Sex-specific growth functions, 242, 256 Somatic growth, 238, 241, 255
Sexual characteristics, secondaiy, 321-322, 324, South Africa, 20, 24, 142, 202, 205
335 South Atlantic Current, 124
Sexual differentiation, temperature-dependent, South Carolina
67, 70, 86-88, 102, C a r e tta c a r e tta , 40, 41, 42, 146, 147
316-317,319 D e r m o c h e ly s c o r iá c e a , 214
Sexual maturity, age of, 263 magnetic inclination angle, 125
Seychelles Islands, 201, 205 Southern Equatorial Cunent, 127
Shape parameter, 260-261 South Pacific, turtle diet, 201
Shape vision, 115 South Yemen, 201
Shark nets, 401 S p a r is o m a , 213
Sharks, 153, 154, 302, 305, 349 SPA (sequential population analysis), 262-263,
Shear resistance, 100-101 266
Shrimp trawls Spatial dispersion pattern, 235
incidental capture in, 20, 23, 219, 354-355, Specialization, pelagic, 11
378,398-400 Species diversity, evolutionary trends and, 4
turtle excluder devices on, 399-400 Spectral sensitivity, 111
Shunting, intracardiac, 282 Sperm
Simultaneous gait, 59 formation of, 330, 331
Site fidelity retention of, by females, 58
foraging sites, 146, 148, 151 Sperm duct, 57
nest sites, 55, 70 Sphargidae, 6
Size Spirorchidaisis, 289, 376
blood flow and, 302 S p o r o tr ic h iu m , 370
breeding and, 54, 139 Sri Lanka, L e p id o c h e ly s o liv á c e a , 208
habitat shifting and, 139, 142, 145, 148, Stage-structured models, dynamic, 264-267
152-153 Static age-stmctured models, 262-263
thermoregulation and, 301, 304, 309-311 Status, of survival, 16-24, 153
Size-at-age functions, 242, 246, 249, 251, 254 Steady-state equations, 298
Size-based growth model, 251-256 Steering, 180
Size-specific growth rate, 242, 243-245, 254-255 Stellar’s Sea Cow, 16
Skeletochronology, 238-241, 248-249 Steroochrotes, 5
Skeleton, 171, 172, 173 S tic h o d a c ty la h a d d o n i, 204
metabolic bone disease, 365-366 Stochastic bet-hedging theory, 256
resorption of, 239-240 Stochastic fluctuations
tagging of, 237 environmental, 257, 267
Skin, 259 population abundance, 234, 267
color, 311 simulation models, 267-268
dermal bacterial infections, 368 Stomach, temperature of, 306
dermal mycotic diseases, 369 Stomach lavage, 200
effects of oil exposure, 393, 395 S to m o lo p h u s m e le a g r is , 209, 214
gray-patch disease, 371 Strandings, 394
430 The Biology of Sea Turtles
W
Wagler, 6 Zakynthos, Greece, 20
Warm-bloodedness, 309 201
Z o s te r a c a p r ic o n i,
Water Z o s te r a m a r in a , 202, 207