Professional Documents
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SC
SC
Chemistry
Project
Session : 2020-2021
A Project Report On
“ digestion of starch by salivary amylase
and effect of temperature
and pH on it”
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Signature of Principal
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Declaration I hereby declare that the project work entitled “ To study the
digestion of starch by salivary amylase and effect of temperature
and pH on it ” , submitted to Department of chemistry,
Kendriya Vidyalaya Khagaria is prepared by me.
SAMUEL KUMAR
Class: XII
th
(Science)5
Acknowledgement
I would like to express a deep sense of thanks and gratitude
to my project guide Mr. S.P. Gupta sir for guiding me immensely
through the course of the project. He always envinced keen
intrest in my project. His constructive advice & constant
motivation have been responsible for the successful completion
of his project.
Last but not the least, I would like to thank all those who had
helped directly or indirectly towards the
completion of this project.
SAMUEL KUMAR
Class- XII
th
(Science)6
AIM “ To study the digestion of Starch b
y salivary amylase
and
effect of temperature
and pH on it. ”7
Objective of the Project Report
The main objective of this chemistry project report is “ To study the digestion
of starch by salivary amylase and effect of temperature and pH on it” .
1. To study the digestion of starch by saliva. 2. To study the effect of
temperature on the digestion of
starch by saliva. 3. To study the effect of pH on the salivary digestion of
starch.8
Introduction Every health book insists on the chewing of food. The act of
chewing stimulates the excretion of saliva. Saliva mixes up
with the food and helps its digestion. That is, the enzyme
ptyalin or amylase present in human saliva hydrolyzes the
big molecules of food into many molecules. For example, starch into mono-
saccnaricies maltose and
glucose; proteins into amino acids and fats into fatty acids
and glycerol. Thus saliva not only helps in digestion of food but also
convert into energy generating substances. Further, enzyme
and their activity are very sensitive to temperature and pH. Even a slight
variation in these two factors, can disturb the
action of enzymes. In other words, digestion of food by
salivary amylase is also effected by temperature and pH and
can be verified experimentally. For example, hydrolysis of starch can be verified
by testing it
with iodine solution. Starch forms blue coloured complex
with iodine. If no starch is present in a system it will not give
blue colour with iodine.9
Apparatus Required
Experiment 1
Aim:- To study the digestion of starch by saliva
Conclusion
Experiment 2
Aim:- To study the effect of temperature on the digestion of
starch by saliva.
Observation
Time passed after
mixing saliva
solution with starch
solution (in min) Intensity of blue colour Test tube 1 at
room
temperature
Test tube 2 at 50°𝑪
Test tube 3 in
boiling water 5 Light blue Blue Deep blue 6 No blue colour Light blue Blue
7 - Faint blue Blue 8 - No blue colour Blue 9 - - Blue 10 - - Blue
Conclusion
Experiment 3
Aim:- To study the effect of pH on the salivary digestion of
starch.
Procedure for Chemistry Experiment 1 is: 1. Take three test tubes and label these
1,2, and 3. 2. Add 5ml of the starch solution, 2ml of the saliva solution
in each test tube. 3. Now as 2ml of water in test tube number 1 2ml of Dilute
HCl in test tube number 2 and 2ml of Dilute NaOH
solution in test tube number 3 and shake carefully. 4. Keep the three test tubes
in water at room temperature
for about 10 minutes. 5. Add 2 drops of the solution of each test tube with 1%
iodine solution and observe the colour change. Observation
Test tube
Solution taken Colour produced (in test
tube) with 1% iodine
solution 1 Starch
+ saliva + water No blue colour 2 Starch
+ saliva +dil. HCl Blue colour 3 Starch
+ saliva + dil. NaOH Blue colour Conclusion
Hydrolysis of starch by the enzyme does not take place in
the acidic or alkaline medium.15
Precaution
1. All apparatus should be clean and washed properly. 2. Add equal drops of
iodine in all the samples while
testing for starch. 3. Temperature variation affects enzyme activity, so take
the readings at a constant temperature for all the
samples. 4. Delay in sampling or the reaction time may affect the
result. So, iodine drops must be added at fixed intervals
in all samples.16
Bibliography
1. Wikipedia-the free encyclopedia 2. http://www.google.com/ 3.
http://en.wikipedia.org 4. www.icbse.com 5. Comprehensive Chemistry 6. Chemistry
NCERT Class XII
th
7. Dinesh chemistry
Thank you!!
-Samuel kumar