Coumarin & Dude ligu chifa coumarin Myc tiéu hoc tip Dinh nghia v8 coumarin Phén loai, cu tric ce 4 foal coumarin chin 3. Céc tinh chat 17 héa, sinh hoc oda coumarin 4. Chiét xuat, phd Igp coumarin ti dug lieu 5. Dinh tinh, dinh tucng coumarin trong dude iéu 6, Tée dung, céng dung cia cde coumarin 7, Cc dude ligu tidu bidu €6 coumarin: (Bach chi, Tn hi, Miu, Sai dat) Prin mot ‘al cudng vé coumarin 1. Kai nig chung 5. Chit xudt & Phin Kip 2. Phin bai 6. Cac ph, php phn tich 3. Bie ciém v8 cBu trie ——_7._Phn BS trong ty nhién 4. Tinh chat 8. Tac dung ~ Céng dung 1, Kat im chung v coumarin CCoumarin thuéc lép phenylpropanold da ving (n > 2) + Phenylpropanoid dan gin (n = 1 véng) + 2cid cinnamic v8 hydroxy-cinnamic - acid coumaric (0, m, p) - acid caffeic, ferulc va dln cht + Phenylpropaneid da ving (n> 1 vang) ~ benzo-a-pyron (coumarin, isocoumarin) ~ benze-y-pyron (flavonoid) ~ benzo--pyron)Vi tri cia nhém coumarin M@t sd phenyl-propanoid dan gian wicnnat eae on eee [ox] -e~ Soe etter com OO OC) fronNgun gc cla coumarin = Ty nhién: Phin lp ti hat Coumaruna odorata (Vogel, 1820) + Téng hop: a. Phan Cng Perkin (1868) 6. stele om ‘ounetin b. Phan Ging Pechmann (1884) hat Bu Tonka ‘BN dog (Coumarouna odorata) (Walotus ofa Pall) an) oD a= (Tel > esnapgengng a Uch sit nghién ctu coumarin Lihat B§u Tonka = vogel (1820) (T2846, Gerare) Coumarouna odorata phn pr Un Sisal Pen (2868) rin ‘AcONA v8.Ac;O cud thé ky 19) tir niu ph, phép coumarin thé 2 Anschutz (1908) 1 4-0H-coumarn Dicoumaral* +1916, Simonis > cua “Chemisty of coumarin” + 1940, KP Link + tin gy must huyét ca Dlcoumarol + 1945: ting hgp Warfarin’, due ding... it crust +1955, Warfarin duge dng tr ching nbd méu etm. Uch si nghién cttu dicoumarol Melos ofcrae a Pauline Read Ans Fabacese (Go01 3578) (aas2- 1937), oka cbk4b ibn ni etc (epegiis.) (1903-1538) (rommara) [ons ams | a sic vi at nyt Warfarin = Coumadint‘Bénh s6 trén khung coumarin ‘Bnh s6 trén khung coumarin (theo 3, 8, Harborne, 1989: s6 phu dira) (theo cfc ti Key Khée: $8 phu di vio) CON, LOO, ON. #A 2. Phin loal céc coumarin* 2. Phin loal céc coumarin Tumananune 2 exyeoumarn_cunbeiferon grémeoumarn... [| prion er D. aleeonyoumarin spre) > |umerreron | enbeteen 1. consiin vio [Unborn CED 3 6,7-ron0 (near porte ron vir jn | salen ergot en) 2 Rran-coumamn |—= | Se-tree onal onsen) Za Feone ing _ Gone [Pe Pantone! (An) ‘sama ma 5 2b. Fano anulr (ea) [angen [age horn Tay wan an sansa 3a. Pyreno lng _@ vb) Prantwitn—_rartet,santonrein 2 Prroneswamarin |-—* | 9. ziepyrane Cenauar: ses) 3, Pyrano ngulr_3 vena | Sessn san 72, wieoumarin Deoumret —_[aleoonarl woman = becoumann ——_(enoraral) “.Phenyreounerin—__[Coueston_[wedelicon - 6. phenyLeoumarin (3/4phony) “4 Hjohencourare _[Coumarel__ [vara aceecsurarl Poumarn pron he 5 tutemegrouer cr ae fe mh coumerin ke 46, Coe coor We‘Coumarin dn gin Cin gol la nhém umbeliferan (Ry H; dc bide chi!) Trong ty nhién, thuting o6 thém nhém OH & 6, 8 2.1, Coumnarin dan gién Aalwcon Re RR Giycosid (Coumarin® on oH - Skimmin Neohyérangin = Uj =U7-0-9% Umbelifeont =H OHH Sage . Escuetie® = oon | Ell + Cac nhém OH nay €6 thé tao O-elycosid vai 1 eng (ale!) cicrrin + Méx sB aglycon coumarin hung o&p: Daprnetin HOH OH | Daphnia ~ coumarin esculetin® ~ scopoletin® poli” OMe Seopa S709 Scopoleti® OMe OHH Fabien $7-29ley1 ~umbeliferon® = heriarin® = fraxiln Fraxatn ‘ole oH OH |Fraxin F804 2.2. nhém Furano-coumarin 2.2. nhém Furano-coumarin + hd 6,7furenocoumarin + nhém 78-urenocoumarn = nhém psoralen (near) = nbém angen (angular) 2.2.1. Phan nhém psoralen (linear) psoralen* bergaptol bergapten en otto O ote OO, ° chy cfu tric oa rano- mae Seno cacto Gay ast hy be, cis (1930s)aphnoretin = (sopeeti-3 -0—7-amblleron) (ts Edgeworth ctrsanth) ee dgeworosid C 55 biescles (c eagonorti cysantta) (Gu vi yedoonis) 244. Becounarin atiecounan — G) ee Eagewerosi 8 (ts Edgeworth orsart) 2.4.2, Phin nhém phenyl-coumarin Sphenybcoumrin <—» resvertal ‘+ phenyrcoumarin wedeloeton Si ait neofavonoid <> calophyod/ miu ‘+ Cac 3:pheny! coumarin ‘Déng chi y nh 1b cdc coumestan nu wedelolacton / S8i dt. Ching c6 thé dude coi nhu Ia c&c dn xuSt tit iso-flavonoié ho ty resveratrol, By Hg “Os, savenois resveravel \Wedeloacton (Si a+ Cc 4-phenyl coumarin ‘Bing chi nt 18 céc calophyllold & inophyllold / hat MO v. Chang c6 thé dutge coi nhu a cfc dn aust ti neo-lavonoid, netavonold ‘aloo nope 2.4.3. Phin nhém alkyl phenyl coumarin + C3 hop chit nly 6Bu c5 hd 4-0H ty do va C1" 1B Ct +6 dng phn R, Svs Nn hp racemic + Warfarin thutzng mala hén hop racemic (dang mu6i Nat). + Dang (S)-warfarn:(1-8-pheny!) céhoat tinh manh go 5 ~ 8 fin 0 vl dang (R}-arfarn: R (Y'-a-pheny!) 2.4.4, Phin nhém aflatoxin BeOS 1D cnens, 1 acne Sine Bine 2 14 chBt (aflatoxin BL, 82, Gl, G2, MI, M2, Q.), do cc nim ‘Aspergilus flaws, A parascus) kj sinh én ng ce sin a “+ Chi: Nguy hiém nh aflatoxin Bx: ay ung thy gan! 2.4.5, Phin nhém acid ellagle cid atoge a gate 6 teote ‘Aid elagc Kh ma 1 hay 2 ving s& xu hin 1 ay 2 nhém COOH ‘Céc nm “COOH nay 58 nl ester vil céc nm -OH cla polyol (ae. Va to thanh rt nhiéu dang ellag-tenin2.4.5, Phin nhém acid ellagic id llag koa COOH) {cd luteke (1 nhém COOH) - an i eer vin “OF | acose (ce elagisannin) HHOP (2 nhén COOH) (eva hyerory aprenc 20) eryerossocourarn hycranganel + Sioh phat nguyén keéng c6ién quan gi vat coumarin, + N6i chung, Rp trong Wy nhign. Mbt ca eyo (+), + Vid hydrangenol, phyloduicn, cic thuberginol A», + 8c cia colon ~168 ppm (8c ea esten / coumarin ~161 ppm) 2.4.7. Céc phan nhém coumarin khée oo gh 8. Coumermyen gun vi rh vat 3, Bic lém cfu tric cla coumarin “+ Trong dude liu, coumarin thutng & dang zglycon hen, 61 kh cing & dang O-alycosid, chua gp dang C-alycosid + hi dang aglycon, hu hat chiing du cb Oxy C7. ‘+ Cac furana- va pyrano-coumarin ludn cb Oxy & C-7 v8 1 ndi di tgp ch vi néi di ca vong benzo ‘+ Kid dang O-aycosid, mach duting: + thuding chi la duting don (da sé la BO-alucose). + itkhila duding khéc (al-rhamnose; BD-apiose) ~ hiém khi la duding di nhur (gle—ole),(Gle—xy).4, Tinh chat ly héa cia coumarin inh cla coumarin | [ 4.2. Héa tinh cle coumarin 4.4. Cim quan 2.1, Ph ing a -OH phenol 4.1.2. Tioh thing hoa 4.2.2. Tinh thing hoa 4.43. Pro UV 2.3 Ph ing dng ma wong lacton 4.264 Phd IR 4.2.4, Ph. ing ting huh quang / UV 4.15, Phd NMR, 2.5. Ph ing vl thus ti azo 4.1.6, Pho MS. 4.2. Ph. ig cing hap val Brom 4.1.1. Tinh chat cdm quan + Thuding k&t tinh khing mau, hoc mau ving nhat + Mui tham ngot, nhung vi thug Iai ding + Pht quang / UV 365 nm cho mau vang dn xenh séng * + Kém bn trong mi truting kiém (do mé vng lacton) ** + Mot sé: kém bn / UV 365, hy (bién tinh / bin méng!) + 6 phn eye: Néi chung rt kém dn kém phn cy ** + Dang aglycon (déng ving): dB tan / cdc d.méi km ph. eye (nhung khi mé ving s® tao muéi phenolat: d8 tan / uc) + Dang glycosid: tan duge / nude nding, cn 4.1.2. Tinh thang hoa “Tung ty cc anthraquinan aglycon dan gin, cc coumarin ddgn gién & dang aglycon th cé thé thing hoa dude. + Céch thy hign: xem ti igu Thue tp duge eu. biog inh wn dd am ip hone > 200-0) 4.1.3. Phé UV cila céc coumarIN (Agayy NM) ‘coumarin don gn [274 30 W708 240, 253, 325 25,7 60H 263 329 167 d-0H 256,262,299 348, 2°87 d-0H 258 335 '5-0Me, 7-08 [247,257 330, 2°6.0Me,7-0H | 252, 259,297 344 1'7-0Me, 60H __|252, 260,298 346 257 d-0Me (245,254 325 2°67 4-0Me — |251, 258,294 342 werarome —_|250 318PHO UV (aye nm / EtOH abs.) cia coumarin Furano-coumarin (psoralen & angelicin): vng ving 350 nm. _stpsoralen (linear) | 205-235 240-255 | 260-270 | 290-316 tangelcn (ara.) 249-255 297-310 Pyrano-coumarin (xanthyltin &sesetin): ving ving 250 rm. _sbxanthyltin (lin) 261-265 347-351 eseselin (ang. | 233-237 283-287 | 343-350 TREO 4.1.4, Phé IR cia coumarin (env) vg lacton propen fién hep vbng thom rind OH 1715-1750 (C=O carbonyl) 1130-1160 _(C-0 carb!) 1625 - 1640 3000 em 3300 ~ 3400 em Cac phd IR sau day du lay tir trang SOBS (Spectral Data Base trén internet, SVP) PhS IR (KBr) cila coumarinsanthotoxn4.1. Ly tinh ciia coumarin 4.1.5, Phd NMR cia coumarin (tham khéo DL I, tr. 51) ‘+ Phd ki NMR (Nuclear Magnetic Resonance Spectroscopy) 1a 1 Ky thuat ove ky hit hu 68 xée dinh eu tric héa hoe ‘ka coumarin cing nhu' cla céc hap chét hit ca. + Ph NMR cia coumarin thi dn gin, 8 tp cn, a 5 coumarin thdng thudng: 5.2 ~ (160-161 ppm) nh coumarin ©) 7-04 (unbelfeor) 6-08 (escuetiny 78-d-08 (saphnein) 164 -70¥e (herria) 2607 -0.fDgIe(ckimmin) 1614 70H, 60Me (copoetn) 161.1 7-00-alescopoen 10,3 1609 16055 + Phd 2H- v8 C-NMR cho théng tn trv we ~ ki khung coumarin (simple, furano, pyrane-coumari..) Gan meee a + cdc mach nn (-, OH, -OMe, OAC, prenl, 0s..) Orson 1200 | ose roy + Vai sy tr cia phd Khdl (MS, Mass Spectroscopy), oMepsolen 104 | mares 1605 Vie xéc dinh eB tréc Bc coumarin str nn rt dng. ste pssalen 1603 | sanhoxeen 1510 . sacoversonkn 1605 | sein 1604 Ph 13C-NMR ca coumarin aryerobergaptenPhé 13C-NMR ciia coumarin PO, > =, Phé 13C-NMR cla coumarin seopatetn “Oo, Poraivoss | cosy Phé 13C-NMR cla hemiarin Ph6 1H-NMR cia esculetinPhé H-NMR ala hemiarin Gié tri J cla 2 H ortho / cdc vong tke is % id nay 181 tng 56 quan trong trong vie gti cB tric ha hoe, Gi tri cia 2 H ortho / cic ving (€ la Khodng cich gia 2 peak, tnh bang He JL tieano phenyl eprron rane Gna) Ge ashe o=35H) on ioH 4.1. Ly tinh ola coumarin 4.4.6. Phé khéi (MS) cla coumarin (nn xem qua) “+ Ph8 kid (MS, Mass Spectroscopy) [8 1 kj thu8t phé nghiém Gt ich trong vige kndo sét cfu thie cfc hop chat hu oa (Tham Kho Duge Iu hoc I, 2011, trang 46-50). “+ Su chénh léch v8 ti s8 m/z (Aye) cho ta kha niém v8 sy c6 m&t cla céc ném chit tr8n khung edu tric. “+ Voi cde cu trite coumarin dan gin, cfc tn higu m/ién quan nM] va [M — 28] ti rt man, dy 8 1 chi du quan trong cela nhém >C=0 (28 4vC) trong ving «pyran,Ph6 kh6I (MS) cha coumarin don olan mom) aries estes) “Tn phé 6 2 tin hiéu menh, chénh nau 28 dan vi CL Phé khél (MS) cla coumarin Phd kht (MS) ca umbelliferon Phé khdl cia 4-OH-coumarinPhé khél ciia esculetin Phé khdl cia 6,7-dimethoxy coumarin ae 60 ~ ve 0: (CHuO, = 206), 7 sen =, 018 miu xanh lemme HoH Cour o5 OH phenol + dd Fe MEM a xan oF4.2.2. Phan Ging tng mau (€éng / mé ving Lacton) anes U U U Co chi dng — mé ving Lacton cla Coumarin kém tan f nue acl tan nue km (ao) (trong) NNguyén tic tinh ché coumarin bing phudng phép acid - base 423. Thitnghiém vi thing hoa (xem myc 4.1.2, slide 48) tens —— ayaa Vs pee . a i Wi . J =o i * we ding) 4+ Bm > king 8 ae) |) + Tod (nu tim) } 4 phn ting diazo (+) 4.2.4, Thif nghiém hujnh quang (trén gly loc) _[ sani e aimin cents aegis segs edacednal epturtas Lapetenaaa eb ns ce DoaneCo ché cia thi? nghigm hujnh quang cumar courainat (5) nh quang Ug nujon quer re ewang mah Ban 4.25. Phan tng vél thud thi azo (Ar-N=N-Cl) Higu ding dinh huéng cla nhém -OH para > ortho >>> meta. » Phin ing thé diazo sB uu tién HG > HB >>> Hes, (0) (00) (mm) Pha dng nay xay ra trong mai trating kiém (pH 9-10), (phai dng @ thuéc thi ciazo, vi thude thir ny c6 tinh acid) PHAN UNG CUA PHENOL & THUGC THY DIAZONIUM a) — “OX os] @> ~ {eee Ph. ing azo ca ee pheno xby ra mal trug kim (pH 9-10) ‘vb uu tién thé tal vit! H para > H ortho >> H meta / OH 4.2.6, Phan Ging véi dung ¢ich nuiée Brom (Cée coumarin thuéing c6 nhém thé & 06 va C7 Khi vi tr C3 v8 C4 khéng c6 nhém thé, A tal ddy d dang cho phan Ging cBng hp véi Brom: Cac fureno- va pyrano-coumerin cling 6 thé cho phn ting cng tung ty tal ni 6 khGng mang ahém th wn cB l Vang ny5. Chit xuft & Ph&n Ip coumarin NN6i chung : d& chidt (do coumarin kém phn cyC). C6 thé chiét coumarin a ksi duige ligu bing céch: 5.1. Ding day dung méi phan eve dn > coumarin s8 ra 6 eS phn doan du. (661 Ki: LBc, thu dich Et,0 > tinh thé 5.2. Ding kiém long ich chigt kiém, cB dc, acd hda > coumarin 5. Chit xuSt & Phan Ip coumarin C6 thé lat tap bang dung dich chi acetat (eri khi coumarin of o-d-OH & Cy, C) + C5 thé phan Iga, tinh ché bng SKC / si-gel. ~ B6i kh: vi thing hoa (néu coumarin bn nhiét) ~ C6 thé theo di coumarin trén si-gel / UV 365 nm (phat huynh quang xenh ducing / xanh lye séng) Phan ip coumarin trong Bach chi a ™ ESSE] Han “To |_ ol women.) COAG | : CE : 6, Binh tinh & Binh lugng coumarin 66.1. Binh tinh vat by coumarin + Phat hujnh quang dé UV 365 am M&i coumarin phat quang -> cdc mau kde nhau, ‘+ Thir nghiém vi thing hoa (ctla aglycon) “Thue té fe kh lam (vi c6 nhigu kj thuét khéc OK hon) + Quang phé (dé phan bet, so sinh, xée dinh efi tic...) = Phd UV = Ph IR = Phd MS = Phé NMR.6.2. S8c ky Ip méng coumarin Thuting dling lép méng silica gel Fas, ‘Dung méi kha trién : kém phan eve EP ~ EtOAc (97:3) ) heran-E0AC (3:1) benzen EtOAc (9:1) CH,-Et0AC (1:1) benzen-aciton (9:1) |) Phat hide ‘Chi yu bing UV 365 nm, quan st mau. ft ki phun thuse ths (nhu ¢iazo, VS.) (6i ki, + acid) 6.2. Séc ky 16p méng coumarin cour m8 | gy ene |S ag a co ham om ® ® © 70H os roweon lessen | @ = 70%,608 fescuin | 28a row eove femin | o¢ == oe, 7-08, feelin [2S rove6or _|bosewoetn | - 20 Tove sone fecwwon | 70M renin | 55st Suen ~ HCODEE~ aca fomie (54 1) 1d, ~ AcOH ~H,0 (4:1 1; lop a) Suen ~ acaton (95 5; kal ih 2 in) oy 6,2, S&c ky Ip méng coumarin scram [seo [PGE a cm fo foe SS cmoer [foeJo a? SSeS 2 (A = tluen~ ety sett (5:1) ~ CHO = MeOH (4:03) benzen ~ ey acetat (7:3) x cur Juen ~€,0 ~ ACOH 1056 (1:2) 6.2, Sic kj I6p méng coumarin ee omonamone | MPR | pana) Fone a ome stone “ some some rinsatin | os (0) = ttven~ E40 ~ AcOH 1056 (L159)6.3. Binh tinh héa hoc coumarin + C6 thé khal thé mol théng tint cic phan Ging héa hoe (em myc 4.2. Héa tinh aia coumarin, slide 85...) Luu y ring: + ting phan ding riéng Ié thi khdng dc higu cho coumarin (mang tinh phi din hon 18 xc nh). ‘+ mau véi dd, FeCl, thi rt nhat (khéc xa flavonoid, tannin + phan (tng 06 ¥ nghia Ging ké: md / ding vong lacton (nha 18 khi Kt hap vdi test hujnh quang ois / trans...) + Nén k&t hop vai cde dinh tinh khée (mau phat quang dui UV 365, SKLM, HPLC, va nhat I cc ky thuat phd nghiém). ‘+ Nén tham khéo muc Binh tinh trong céc chuyén lug cu thé (vid chuyén lun Bach chi, Tién Hd trong DBVN...) 6, Binh tinh & Binh luwgng coumarin 6.4. Binh lugng ce coumarin 164.1. Phuong phap oxy héa ~ knit (kém chinh xc) Coumarin + d.dich KMInO, / H,SO, > mat mau. rm@t giot thiva dung cich KMnO, > tim Héng. 6.4.2. Phuong php do phd UV (cin méu tinh Knit) ‘Bo // vai mu chu ta Apaw Qh hn Abs. So sénh véi chun -> [coumarin] / mu 64.3, Phung phép HPLC (théng dung) Vidy, tham kro HPLC dich chiét Tlén Wé Peucedanum decursivum, CCbt: RP-Spherigel ODS C18 (250 x 4.6 mm, 5 um); Pha dng: AcCN ~ H,0 (gradient mode): (0-24 min: 50% AcCN. 24-40 min: 65% AcCN, Tc d dong: 1.5 ml phit, Phit hign tai 254 nm. Ref: Journal of Chromatography A, 1076, (2005), pp. 127-132. (xem slide k8) HPLC dich chigt Tn Wi Peucedanum decursivum7. Sif phn b6 cia coumarin trong ty nhién ‘88h nim 2013, * coumarin ty nin: > 1300 chat, chit yBu ti # 150 ho thy vat. + coumarin (bén) tng hap: rat nhiéu, 1 DL thuting chi chi 1-2 loa / nhém coumarin (furan, pyrano-) ‘Vi nhiBu cht e6 eu tr tung ty, ‘alles ‘Apiaceae Rutales Rutaceae Asterales, Asteraceae Fabales Fabaceae Oleales Oleaceae Urticales Moraceze Trymelacales Thymelaeaceae M@t s thy vat gidu coumarin Nun i Counarn (%) ‘nh dBc Qué (Cnnamomum casa) ~ 11% ‘inh dB Que. zeylanicur) 146 ti Baw Qu (C 2etanicum) Tat DBs Tonka (Coumrouna odorata) ANNio hug (Mellons ofcinals) 0325-056 cy Lavandr (Lavandula spp.) 5-8% ih bu iy Fol (Lalum perenne) % gon Wer (Calum ederatu) rit oau hat miu (Clap nop) ‘nh a Bac ha (Mentha arvensis) 20 ppm 1a 18 xan (Camella chines) 2 tppm‘Tée dyng & Céng dyng cia coumarin ‘+ Lim thu chéng dng méu (tr ching huyét khéi) ~ Aceno-coumaral (Sintrom*) - Phenprocourmon (Marcoumar*) + Dicoumarol; Warfarin (Coumadin*, Coumnarol*). thuting phdi hgp vét Aspirin 81 mg, Heparin ‘Ha st (lim din mach ngoai bién): Bach chi ‘+ Dan mach vanh, chéng co that: Visnacin ‘+ Chita chiing bach bién, vay nén: Bsoralen, Angelicn (+ UVA) + Khang khusn, Khang viém: Wedelolacton / Sai dt (Chit: Nhi8u coumarin lai cé téc hal (iém da, t8n thusng gan sinh ung thu). o ‘Warfarin = Coumadin = Jantoven = Marevan = Uniwarfin siz S6666 = S608 (Co chi chéng dng méu cla Warfarin Vi. K dg epond (ana heat ang) Protein S&C ‘ave mu dog Phenprocoumon = Marcournar® ‘Bugc ting hap ti 4-OH-coumarin v8 phenyl alkyl alcol PhenprecourenPhenprocoumon = Marcoumar® oxo + La thudc king dng méu, dang using téc dung kéo dé. + Gx chi khing dng tuong ty nu nhém warfarin: 46 khng vi. K theo kiu de ché 1 enzym o5 ténI8 vit, K epoxid reductase, ‘qua 46 ng chén sy hoat héa (v dang kin) ca vit. K epoxid + Kak qua ld cc yu t5 oy Ong méu (Il, VI, DX, Xen) kN 20% (ngdm lanh véi EtOH 50%; tham khio thém trang 912) ‘Téc dung & Cong dung “Tang tiét ich auitng hé hp Ding chita ching khé thd, ho, viém hi hap CChéng sy’ ngung két tidu cu (nodakenin, nodakenetin, decurosid II, decurosid 1V) Ding lm thud gin mach vanh,cay Mau. Calophyllum inophyilum Clusiaceae x cay Miu Calophyilum inophyllum Clusiaceae cay Mau. Calophyllum inophyllum Clusiaceae Cay MauTHANH PHAN HOA HQC CUA HAT MU - U 1. Phin khéng tan / cBn: Du béo (quan trong!) 2. Ph8n tan trong cBn: + tinh du, nha + céc xanthonoid (calophylin 8, jacareubin...) + cdc dn chat 4phenyl coumarin (rét nhiu chét) ‘+ Cle ¢phenyl coumarin / hat Miu Céc calophylolid & inophyllolid / hat Mi u o5 thé duge coi nhu la céc dan xuat tif neo-flavonoid, - calophylolid ~ inophyillid nee Aaveolé alphas sophia ~ acid calophyllic - acid calophynic.. TAC DUNG VA CONG DUNG HANH PHAN CHINH CUA DAU HAT MU U Ham lugng (%) cdc acid béo / Bu Miu Palmitic 147 Oleic. «49.4 -—Linolenic 0.30 Stearic 12.7 Linolele 21.3 Eleasenoie 1.70 (DAU HAT MU U) ~ khang viém, chéng loét (Phu khoa, Tiéu duéng}...) ~ chifa phéng (Ita, din, nutéc séi, acid ...) = chifa vet thuoing hd, chifa nuéc an chén ~ lam mau in da non, mau [anh seo, lam seo més = sif dung trong diéu tri bénh Hansen (phy tro) Ché phim : Calino, Balsino, Mecalin (+ Tram)Wedelia chinensis Asteraceae ‘Thanh phn héa hoc cia S8I ast £86 phn ding: ‘Thanh phan trén mat d3t, Cé chia nhiéu hgp chat: ‘iedecumarn | 2 cbetavnad | 3.e/elaadd qine [4c aime (crim coven) |>apgenn ae. 3-deateoy @ |= sexquterpenod swedebicon—|+tzsoln [ac 25-dcateoy Q |+aterpenaa s omvedebiacon [steerer |eac.ASdcaen Q | teens + ns nore |= quretn + asroaln a oan ems vo co Cte: Us: onadeblacton wedeolacon eto * spgenin + Asotin + oquerdin tryperin cd nornedeltic B® a) oH Owe OHO oma of Ove of on ‘TAC DUNG ~ CONG DUNG GUA SAI DAT = khang vim, khéng khudn, ~ tri cde vét thuiong nhiém khusn = phng va tri myn nhot, mén naif, rm say. ~ lai dc gan, bao vé té bao gan ‘Sai dat khéng dc (cé thé dling nhu rau) HET BAT COUMARIN & DUGC LIEU CHUA COUMARIN